Assessment of genotoxic and molecular mechanisms of cancer risk in smoking and smokeless tobacco users

Chandirasekar R 1 , Kumar BL 2 , Sasikala K 3 , Jayakumar R 4 , Suresh K 3 , Venkatesan R 3 Show all authors , Jacob R 3 , Krishnapriya EK 3 , Kavitha H 2 , Ganesh GK 5

Affiliations 

  • 1 Unit of Human Genetics, Department of Zoology, Bharathiar University, Coimbatore 641 046, Tamilnadu, India. Electronic address: genechandru@gmail.com
  • 2 Departments of Biotechnology and Zoology, Kongunadu Arts and Science College, Coimbatore 641 029, India
  • 3 Unit of Human Genetics, Department of Zoology, Bharathiar University, Coimbatore 641 046, Tamilnadu, India
  • 4 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
  • 5 Department of Bioinformatics, Bharathiar University, Coimbatore 641 046, Tamilnadu, India
Mutat Res Genet Toxicol Environ Mutagen, 2014 Jun;767:21-7.
PMID: 24769293 DOI: 10.1016/j.mrgentox.2014.04.007

Abstract

Inexpensive forms of tobacco are widely used in developing countries such as India. We have evaluated genotoxicity endpoints (chromosome aberrations, micronucleus frequency, comet assay) and polymorphisms of the XRCC1 and p53 genes among smokers and smokeless tobacco (SLT) users in rural Tamilnadu, South India. Cytogenetic, DNA damage and SNP analyses were performed on peripheral blood samples; micronucleus frequency was measured in peripheral blood and buccal mucosa exfoliated cells. Both categories of tobacco users had elevated levels of genotoxic damage. SNP analysis of tobacco users revealed that 17% carry the XRCC1 gln399gln genotype and 19% carry the p53 pro72pro genotype. Both genotypes are associated with increased risk of cancer.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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