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CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries

Luthfi M 1 , Oki AS 1 , Indrawati R 1 , Rifai M 2 , Dachlan YP 3 , Razak FA 4

Affiliations 

  • 1 Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
  • 2 Department of Physiology, Cell Culture and Animal Development, Faculty of Sciences, Brawijaya University, Malang, Indonesia
  • 3 Department of Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
  • 4 Department of Oral and Craniofacial Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia
Eur J Dent, 2020 Jul;14(3):386-392.
PMID: 32645730 DOI: 10.1055/s-0040-1713704

Abstract

OBJECTIVES:  To analyze CD35/CD89 expression ratio on the surface of neutrophils as an early detection marker for S-ECC.

MATERIALS AND METHODS:  Saliva was collected from 4- to 6-year-old kindergarten students. Salivary neutrophils were obtained by instructing the subjects to rinse their mouth with 1 mL of sterile 1.5% NaCl for 30 seconds before expectorating it into a sterile glass. The expression of CFSE+CD35+ and CFSE+CD89+was measured and analyzed using flow cytometry.

RESULTS: The expression of CFSE+CD89+ in the caries-free group (2.46 ± 0.39) was significantly lower than that in the S-ECC group (3.41 ± 1.11), with a p-value of 0.0001, while the expression of CFSE+CD35+ in the caries-free group was (2.35 ± 0.56) compared with (1.54 ± 0.35) (p = 0.0001) in the S-ECC group.

CONCLUSIONS:  The expression ratio of CFSE+CD89+ and CFSE+CD35+constitutes a marker for S-ECC.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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