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Fulltext Perception of hypertension management by patients and doctors in Asia: potential to improve blood pressure control

Abdul Rahman AR, Wang JG, Kwong GM, Morales DD, Sritara P, Sukmawan R, et al.

Asia Pac Fam Med, 2015;14(1):2.
PMID: 25729324 DOI: 10.1186/s12930-015-0018-3

BACKGROUND: Hypertension is one of the world's most common health conditions and is a leading risk factor for mortality. Although blood pressure can be modified, there is a large proportion of patients whose blood pressure remains uncontrolled. The aim of this study, termed Edvantage 360°, was to gain a deeper understanding of hypertension management in Asia from the perspective of patients and doctors, and to propose strategies to improve blood pressure control.
METHODS: Conducted in Hong Kong, Indonesia, Malaysia, the Philippines, South Korea, Taiwan, and Thailand, Edvantage 360° was a mixed-methods observational study that used both qualitative and quantitative elements: qualitative interviews and focus groups with patients (N = 110), quantitative interviews with patients (N = 709), and qualitative interviews with doctors (N = 85).
RESULTS: This study found that, although there is good understanding of the causes and consequences of hypertension among Asian patients, there is a lack of urgency to control blood pressure. Doctors and patients have different expectations of each other and a divergent view on what constitutes successful hypertension management. We also identified a fundamental gap between the beliefs of doctors and patients as to who should be most responsible for the patients' hypertension management. In addition, because patients find it difficult to comply with lifestyle modifications (often because of a decreased understanding of the changes required), adherence to medication regimens may be less of a limiting factor than doctors believe.
CONCLUSIONS: Doctors may provide better care by aligning with their patients on a common understanding of successful hypertension management. Doctors may also find it helpful to provide a more personalized explanation of any needed lifestyle modifications. The willingness of the doctor to adjust their patient interaction style to form a 'doctor-patient team' is important. In addition, we recommend that doctors should not attribute ineffectiveness of the treatment plan to patient non-adherence to medications, but rather adjust the medication regimen as needed.
KEYWORDS: Attitude to health; Hong Kong; Hypertension; Indonesia; Malaysia; Philippines; Qualitative research; South Korea; Taiwan; Thailand
Fulltext Thermoluminescence dating of a Lembah Bujang structure

Rosli H. Mahat, Nur Maisarah Abdul Rashid, Mohd Jalaluddin Jasman, Zulkifli Jaafar

Journal of Nuclear and Related Technologies, 2010;2010(2):66-68.
MyJurnal

The Lembah Bujang archeological complex near Sungai Petani, Kedah consists of various structures constructed at different times and spread over a wide area. This paper reports on the thermoluminescence (TL) dating of one of these structures. The structure was found to be 350 ± 90 yrs old. This is very young as compared with other structures that are from the 4th to the 16th centuries. This structure is suspected to be remnant of a Muslim Mosque whereas the other structures were Hindu and Buddist temples.
Diabetic wound healing of aloe vera major phytoconstituents through TGF-β1 suppression via in-silico docking, molecular dynamic simulation and pharmacokinetic studies

Roney M, Issahaku AR, Govinden U, Gazali AM, Aluwi MFFM, Zamri NB

J Biomol Struct Dyn, 2023 Nov 09.
PMID: 37942697 DOI: 10.1080/07391102.2023.2279280

To restore the integrity of the skin and subcutaneous tissue, the wound healing process involves a complex series of well-orchestrated biochemical and cellular events. Due to the existence of various active components, accessibility and few side effects, some plant extracts and their phytoconstituents are recognised as viable options for wound healing agents. To find possible inhibitors of diabetic wound healing, four main constituents of aloe vera were identified from the literature. TGF-β1 and the compounds were studied using molecular docking to see how they interacted with the active site of target protein (PDB ID: 6B8Y). The pharmacokinetics investigation of the aloe emodin with the highest dock score complied with all the Lipinski's rule of five and pharmacokinetics criteria. Conformational change in the docked complex of Aloe emodin was investigated with the Amber simulation software, via a molecular dynamic (MD) simulation. The MD simulations of aloe emodin bound to TGF-β1 showed the significant structural rotations and twists occurring from 0 to 200 ns. The estimate of the aloe emodin-TGF-β1 complex's binding free energy has also been done using MM-PBSA/GBSA techniques. Additionally, aloe emodin has a wide range of enzymatic activities since their probability active (Pa) values is >0.700. 'Aloe emodin', an active extract of aloe vera, has been identified as the key chemical in the current investigation that can inhibit diabetic wound healing. Both in-vitro and in-vivo experiments will be used in a wet lab to confirm the current computational findings.Communicated by Ramaswamy H. Sarma.
Fulltext Clinical and bacteriological profile of diabetic foot infections in a tertiary care

Goh TC, Bajuri MY, C Nadarajah S, Abdul Rashid AH, Baharuddin S, Zamri KS

J Foot Ankle Res, 2020 Jun 16;13(1):36.
PMID: 32546270 DOI: 10.1186/s13047-020-00406-y

BACKGROUND: Diabetic foot infection is a worldwide health problem is commonly encountered in daily practice. This study was conducted to identify the microbiological profile and antibiotic sensitivity patterns of causative agents identified from diabetic foot infections (DFIs). In addition, the assessment included probable risk factors contributing to infection of ulcers that harbour multidrug-resistant organisms (MDROs) and their outcomes.
METHODS: We carried out a prospective analysis based on the DFI samples collected from 2016 till 2018. Specimens were cultured with optimal techniques in addition to antibiotic susceptibility based on recommendations from The Clinical and Laboratory Standards Institute (CLSI). A total of 1040 pathogens were isolated with an average of 1.9 pathogens per lesion in 550 patients who were identified with having DFIs during this interval.
RESULTS: A higher percentage of Gram-negative pathogens (54%) were identified as compared with Gram-positive pathogens (33%) or anaerobes (12%). A total of 85% of the patients were found to have polymicrobial infections. Pseudomonas aeruginosa (19%), Staphylococcus aureus (11%) and Bacteroides species (8%) appeared to be the predominant organisms isolated. In the management of Gram-positive bacteria, the most efficacious treatment was seen with the use of Vancomycin, while Imipenem and Amikacin proved to be effective in the treatment of Gram-negative bacteria.
CONCLUSION: DFI's are common among Malaysians with diabetes, with a majority of cases displaying polymicrobial aetiology with multi-drug resistant isolates. The data obtained from this study will be valuable in aiding future empirical treatment guidelines in the treatment of DFIs. This study investigated the microbiology of DFIs and their resistance to antibiotics in patients with DFIs that were managed at a Tertiary Care Centre in Malaysia.
Development of Low-Cost Microcontroller-Based Interface for Data Acquisition and Control of Microbioreactor Operation

Husain AR, Hadad Y, Zainal Alam MN

J Lab Autom, 2016 Oct;21(5):660-70.
PMID: 26185253 DOI: 10.1177/2211068215594770

This article presents the development of a low-cost microcontroller-based interface for a microbioreactor operation. An Arduino MEGA 2560 board with 54 digital input/outputs, including 15 pulse-width-modulation outputs, has been chosen to perform the acquisition and control of the microbioreactor. The microbioreactor (volume = 800 µL) was made of poly(dimethylsiloxane) and poly(methylmethacrylate) polymers. The reactor was built to be equipped with sensors and actuators for the control of reactor temperature and the mixing speed. The article discusses the circuit of the microcontroller-based platform, describes the signal conditioning steps, and evaluates the capacity of the proposed low-cost microcontroller-based interface in terms of control accuracy and system responses. It is demonstrated that the proposed microcontroller-based platform is able to operate parallel microbioreactor operation with satisfactory performances. Control accuracy at a deviation less than 5% of the set-point values and responses in the range of few seconds have been recorded.
Combination of Multiple Ligation-Dependent Probe Amplification and Illumina MiSeq Amplicon Sequencing for TSC1/TSC2 Gene Analyses in Patients with Tuberous Sclerosis Complex

Ismail NF, Rani AQ, Nik Abdul Malik NM, Boon Hock C, Mohd Azlan SN, Abdul Razak S, et al.

J Mol Diagn, 2017 03;19(2):265-276.
PMID: 28087349 DOI: 10.1016/j.jmoldx.2016.10.009

Tuberous sclerosis complex (TSC) is an autosomal dominant neurocutaneous disorder characterized by tumor growth in multiple organs and caused by mutations in either TSC1 or TSC2 genes. Because of their relatively large genomic sizes, absence of hotspots, and common type of mutations, mutation detection in TSC1 and TSC2 genes has been challenging. We devised a combination of multiple ligation-dependent probe amplification (MLPA) and amplicon sequencing (AS) to simplify the detection strategy, yet we come up with reasonably high detection rate. Thirty-four Malaysian patients diagnosed with TSC were referred to Human Genome Center, Universiti Sains Malaysia. We used a combination of MLPA to detect large copy number changes and AS to detect smaller mutations. TSC1 pathogenic or likely pathogenic mutations were found in 6 patients (18%) and TSC2 in 21 patients (62%), whereas 6 patients (18%) show no mutations and 1 patient (2%) showed only TSC2 missense variant with uncertain significance. Six of the mutations are novel. Our detection strategy costs 81% less and require 1 working week less than the conventional strategy. Confirmatory sequencing using Sanger method on a few representative mutations showed agreement with results of the AS. Combination of MLPA and Illumina MiSeq AS provides a simplified strategy and reasonably high detection rate for TSC1/TSC2 mutation, which suggested application of the strategies into clinical molecular diagnostics.
Fulltext Systemic and coronary levels of CRP, MPO, sCD40L and PlGF in patients with coronary artery disease

Fong SW, Few LL, See Too WC, Khoo BY, Nik Ibrahim NN, Yahaya SA, et al.

BMC Res Notes, 2015;8:679.
PMID: 26576922 DOI: 10.1186/s13104-015-1677-8

Biomarkers play a pivotal role in the diagnosis and management of patients with acute coronary syndrome. This study aimed to investigate the differences in level of several biomarkers, i.e. C-reactive protein, myeloperoxidase, soluble CD40 ligand and placental growth factor, between acute coronary syndrome and chronic stable angina patients. The relationship between these biomarkers in the coronary circulation and systemic circulation was also investigated.
Mitigation of NOx emission using aromatic and phenolic antioxidant-treated biodiesel blends in a multi-cylinder diesel engine

Adam IK, Heikal M, Aziz ARA, Yusup S

Environ Sci Pollut Res Int, 2018 Oct;25(28):28500-28516.
PMID: 30088249 DOI: 10.1007/s11356-018-2863-8

The present work analyzes the effect of antioxidants on engine combustion performance of a multi-cylinder diesel engine fueled with PB30 and PB50 (30 and 50 vol.% palm biodiesel (PB)). Four antioxidants namely N,N'-diphenyl-1,4-phenylenediamine (DPPD), N-phenyl-1,4-phenylenediamine (NPPD), 2(3)-tert-Butyl-4-methoxyphenol (BHA), and 2-tert-butylbenzene-1,4-diol (TBHQ) were added at concentrations of 1000 and 2000 ppm to PB30 and PB50. TBHQ showed the highest activity in increasing oxidation stability in both PB30 and PB50 followed by BHA, DPPD, and NPPD respectively, without any negative effect on physical properties. Compared to diesel fuel, PB blends showed 4.61-6.45% lower brake power (BP), 5.90-8.69% higher brake specific fuel consumption (BSFC), 9.64-11.43% higher maximum in cylinder pressure, and 7.76-12.51% higher NO emissions. Carbon monoxide (CO), hydrocarbon (HC), and smoke opacity were reduced by 36.78-43.56%, 44.12-58.21%, and 42.59-63.94%, respectively, than diesel fuel. The start of combustion angles (SOC) of PB blends was - 13.2 to - 15.6 °CA BTDC, but the combustion delays were 5.4-7.8 °CA short compared to diesel fuel which were - 10 °CA BTDC and 11°CA respectively. Antioxidant fuels of PB showed higher BP (1.81-5.32%), CO (8.41-24.60%), and HC (13.51-37.35%) with lower BSFC (1.67-7.68%), NO (4.32-11.53%), maximum in cylinder pressure (2.33-4.91%) and peak heat release rates (HRR) (3.25-11.41%) than baseline fuel of PB. Similar SOC of - 13 to - 14 °CA BTDC was observed for PB blended fuels and antioxidants. It can be concluded that antioxidants' addition is effective in increasing the oxidation stability and in controlling the NOx emissions of palm biodiesel fuelled diesel engine.
Fulltext Evaluation of a training programme for non-health professionals as oral health educators

Seman K, Yaacob H, Hamid AM, Ismail AR, Yusoff A

Malays J Med Sci, 2008 Apr;15(2):33-8.
PMID: 22589623

Involvement of oral health educators among non-health professionals in oral health promotion is important in the prevention of oral diseases. This study was carried out to compare the level of oral health knowledge among pre-school teachers before and after oral health seminar. Pre-test data was collected by distributing questionnaire to pre-school teachers in Pasir Mas, who attended the seminar on "Oral Health" (n=33) and they were required to fill anonymously before the seminar started. The questions consisted of information on general background, perceived oral health status, oral health knowledge and the environment where they work. After two weeks, post-test data was collected using the same structured questionnaire and identification code was used to match the pre and post data. SPSS 11.5 was use for statistical analysis. Two out of 33 eligible preschool teachers were considered non-respondents due to absenteeism during the post-test data collection. The response rate was 94.0% (n = 31). The study shows a significant improvement in oral health knowledge among pre-school teachers in Pasir Mas, after seminar (p < 0.001) as compared to controls. Thus, we can conclude that the oral health programme (seminar) appeared effective at influencing oral health educator's knowledge towards oral health.
Fulltext Case report: Incomplete penetrance of autosomal dominant myotonia congenita caused by a rare CLCN1 variant c.1667T>A (p.I556N) in a Malaysian family

Musa NH, Thilakavathy K, Mohamad NA, Kennerson ML, Inche Mat LN, Loh WC, et al.

Front Genet, 2022;13:972007.
PMID: 36659963 DOI: 10.3389/fgene.2022.972007

Myotonia congenita (MC) is a rare neuromuscular disease caused by mutations within the CLCN1 gene encoding skeletal muscle chloride channels. MC is characterized by delayed muscle relaxation during contraction, resulting in muscle stiffness. There is a lack of MC case reports and data on the prevalence among Malaysians. We report a clinical case of a 50-year-old woman presents with muscle stiffness and cramp episodes that started in early childhood. She had difficulty initiating muscle movement and presented with transient muscle weakness after rest, which usually improved after repeated contraction (warm-up phenomenon). She was diagnosed with MC after myotonic discharge on electromyography (EMG). Her brother had similar symptoms; however, no additional family members showed MC symptoms. Serum creatine kinase levels were elevated in both the proband and her brother with 447 U/L and 228 U/L recorded, respectively. Genetic analysis by whole-exome sequencing (WES) revealed a previously reported pathogenic CLCN1 gene variant c.1667T>A (p.I556N). Genetic screening of all family members revealed that the same variant was observed in the children of both the proband and her brother; however, the children did not present with either clinical or electrophysiological MC symptoms. The multiplex ligation-dependent probe amplification (MLPA) analysis conducted identified neither exon deletion nor duplication in CLCN1. In conclusion, this report describes the first case of MC in Malaysia in which incomplete penetrance observed in this family is caused by a known pathogenic CLCN1 variant.
Fulltext Systematical study of multi-walled carbon nanotube nanofluids based disposed transformer oil

Suhaimi NS, Md Din MF, Ishak MT, Abdul Rahman AR, Mohd Ariffin M, Hashim N', et al.

Sci Rep, 2020 Dec 02;10(1):20984.
PMID: 33268816 DOI: 10.1038/s41598-020-77810-8

In this paper, the electrical, dielectric, Raman and small angle X-ray scattering (SAXS) structure behavior of disposed transformer oil in the presence of multi-walled carbon nanotube (MWCNT) were systematically tested to verify their versatility for preparing better alternative transformer oil in future. MWCNT nanofluids are prepared using a two-step method with concentrations ranging from 0.00 to 0.02 g/L. The test results reveal that 0.005 g/L concentration possesses the most optimum performance based on the electrical (AC breakdown and lightning impulse) and dielectric (permittivity, dissipation factor and resistivity) behavior. According to the trend of AC breakdown strength and lightning impulse pattern, there were 212.58% and 40.01% enhancement indicated for 0.005 g/L concentration compared to the disposed transformer oil. The presence of MWCNT also yielding to the decrement of dissipation factor, increased on permittivity and resistivity behavior of disposed transformer oil which reflected to the performance of electrical properties. Furthermore, it is found that these features correlated to the structural properties as systematically verify by Raman and SAXS analysis study.
Fulltext Perceptions of Quality of Life during the Pandemic: A Case Study on B40 Single Mothers

Zakaria SM, Abdullah N, Md Akhir N, Amin AS, Mohd Shukry ANA, Abdul Rashid MR, et al.

Int J Environ Res Public Health, 2022 Sep 27;19(19).
PMID: 36231521 DOI: 10.3390/ijerph191912219

During the pandemic, the lives of B40 single mothers were severely affected, especially in terms of social, economic, and psychological factors. The reduction of income caused by the crisis has forced single mothers and their children to live frugally and without luxury. They also had to perform more than one job at a time during the pandemic to meet their children's needs. A qualitative study has been conducted to explore the perceptions of quality of life during the pandemic among B40 single mothers. Respondents were selected based on demographic characteristics established before the study. A focus group discussion has been conducted on ten (10) B40 single mothers in Balik Pulau, Penang. Single mothers were selected based on purposive sampling. They had to meet the inclusive criteria required to participate in the study, specifically: aged between 20 and 60 years old, belonged to the B40 income category, had children under 18 years old living together, and became single mothers due to divorce or death of husbands. The focused group discussion explored issues related to qualities of life during the pandemic. B40 single mothers expressed their concern about access to health facilities, security in residence, children's education, and economic stability. These areas of life quality have been significantly affected especially during Movement Control Order (MCO). In short, the quality of life of these single mothers has been significantly affected by the pandemic. Their vulnerability towards stress, anxiety, and depression have worsened due to financial issues. Besides the need for emotional and social support, this study found that these single mothers entail financial support.
Fulltext Response surface methodology for optimization of nitrocellulose preparation from nata de coco bacterial cellulose for propellant formulation

Jori Roslan N, Jamal SH, Abdul Rashid JI, Norrrahim MNF, Ong KK, Wan Yunus WMZ

Heliyon, 2024 Feb 29;10(4):e25993.
PMID: 38380021 DOI: 10.1016/j.heliyon.2024.e25993

Nitrocellulose (NC) has garnered significant interest among researchers due to its versatile applications, contingent upon the degree of nitration that modifies the cellulose structure. For instance, NC with a high nitrogen content, exceeding 12.5%, finds utility as a key ingredient in propellant formulations, while variants with lower nitrogen content prove suitable for a range of other applications, including the formulation of printing inks, varnishes, and coatings. This communication aims to present the outcomes of our efforts to optimize the nitration reaction of bacterial cellulose to produce high-nitrogen-content NC, employing the response surface methodology (RSM). Our investigation delves into the influence of the mole ratio of sulfuric and nitric acids, reaction temperature, and nitration duration on the nitrogen content of the resultant products. Utilizing a central composite design (CCD), we identified the optimal conditions for NC synthesis. Analysis of variance (ANOVA) underscored the substantial impact of these reaction conditions on the percentage of nitrogen content (%N) yield. By implementing the predicted optimal conditions-namely, a H2SO4:HNO3 mole ratio of 3:1, a reaction temperature of 35 °C, and a reaction period of 22 min-we successfully produced NC with a nitrogen content of 12.64%. Characterization of these products encompassed elemental analysis, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and field emission scanning electron microscopy (FESEM).
Fulltext High-frequency repetitive transcranial magnetic stimulation at dorsolateral prefrontal cortex for migraine prevention: A protocol for a systematic review of controlled trials

Mohamad Safiai NI, Mohamad NA, Basri H, Inche Mat LN, Hoo FK, Abdul Rashid AM, et al.

PLoS One, 2021;16(6):e0251528.
PMID: 34138860 DOI: 10.1371/journal.pone.0251528

BACKGROUND: Migraine may lead to a negative impact on the patients' quality of life with a subsequent substantial burden to society. Therapy options for treatment and prevention of migraine have progressed over the years and repetitive transcranial magnetic stimulation (rTMS) is one of the promising non-pharmacological options. It induces and alters electric current in the brain via repetitive non-invasive brain stimulation in high frequency. In migraine patients, two common stimulation sites are the M1 cortex and dorsolateral prefrontal cortex (DLPFC). The mechanism on how rTMS exerts therapeutic effects on migraine is not fully established, but the main postulation is that the neuromodulation via high-frequency rTMS (hf-rTMS) might inhibit pain perception. However, evidence from studies has been conflicting, thus the usefulness of hf-rTMS as migraine preventive treatment is still uncertain at this moment.
METHODS: This is a systematic review protocol describing essential reporting items based on the PRISMA for systematic review protocols (PRISMA-P) (Registration number: CRD42020220636). We aim to review the effectiveness, tolerability, and safety of hf-rTMS at DLPFC in randomised controlled trials (RCTs) as migraine prophylactic treatment. We will search Scopus, Cumulative Index to Nursing and Allied Health Literature Plus, PubMed, Cochrane Central Register of Controlled Trials and Biomed Central for relevant articles from randomised controlled clinical trials that used hf-rTMS applied at DLPFC for the treatment of migraine. The risk of bias will be assessed using the version 2 "Risk of bias" tool from Cochrane Handbook for Systematic Reviews of Interventions Version 6.1. We will investigate the evidence on efficacy, tolerability and safety and we will compare the outcomes between the hf-rTMS intervention and sham groups.
DISCUSSION: This systematic review will further determine the efficacy, safety, and tolerability of hf-rTMS applied at DLPFC for migraine prophylaxis. It will provide additional data for health practitioners and policymakers about the usefulness of hf-rTMS for migraine preventive treatment.
Fulltext Analysis of turnover intention among manufacturing technicians in electronic industry

Abdul Rashid Ismail, Wan Normeza Wan Zakaria

Journal of Advanced Research Design, 2017;37(1):1-8.
MyJurnal

This study aims to examine the influences of the plan to further study, career growth
and discriminatory treatment on turnover intention among technicians in electronic
industry in Malaysia. The objectives are: (i) To identify the relationship between the
plan to further study and turnover intention among factory technicians, (ii) To identify
the relationship between career growth and turnover intention among factory
technicians, and (iii)To identify the relationship between discriminatory treatment
factors and turnover intention among factory technicians. The population involved in
this study were the manufacturing technicians at an electronic factory. Survey
questionnaires were used to collect data. A total of 110 questionnaires were analyzed.
Pearson correlation coefficient and regression analysis were used to measure the
degree of relationship between variables. The findings showed that all independent
variables; plan to further study, career growth and discriminatory treatment, were
positive moderately correlated with turnover intention.
Evaluation of tumour cells damage following radiotherapy by TC-99M pertechnetate

Muhammad Afiq Khairil Anuar, Siti Zanariah Ab Aziz, Raizulnasuha Abdul Rashid, Safri Zainal Abidin, Norhayati Dollah, Wan Nordiana A. Abd Rahman

Journal of Nuclear and Related Technologies, 2015;12(2):28-37.
MyJurnal

Radiotherapy has become the most important modality in treating cancer with approximately 50% of
cancer patient undergo the treatment. However, more improvement to the radiotherapy treatment
efficacy is required to deprive cancer. Assessment of tumor progress during treatment is important, to
accommodate the changes that occur during the fractionation course. The objective of this study is to
assess tumor cell damage after external beam radiotherapy by using technetium-99m
pertechnetate (99mTcOf) as a tracer. In this study, HeLa cells were irradiated with 6 MVphoton beam
with different radiation dose ranging from 0.5 Gy to 10 Gy. The irradiated cells were recultured in 6-
well plates and incubated for 10 days. After that, 2 mCi of 99mTcOf were prescribed to each cell
colonies. The viable cells were separated from the rest, and measured for 99mTcOf uptake using singlehead
gamma camera with LEHR collimation. As results, the cells survival, fractions clearly indicate
diminishing effect, to the cells at, higher dose of irradiation. Good correlation were observed between
mmTcGi uptake and survival, fraction for cells irradiated at, lower dose and less significant, correlation
were indicated at higher dose. In conclusion, there is potential for the efficacy of external beam
radiotherapy in treating cancer to be assessed by using radioisotope as a non-invasive tracer. In this
case, technetium-99m, pertechnetate (99mTcOjt) could be attached to the specific antibody so that, better
correlation, between, the cells uptake and possible cell damages could be observed.
Intercellular uptake of technetium-99M pertechnetate by different types of cell lines

Safri Zainal Abidin, Raizulnasuha Abdul Rashid, Muhammad Afiq Khairil Anuar, Wan Nordiana A. Abd Rahman

Journal of Nuclear and Related Technologies, 2015;12(2):33-37.
MyJurnal

The purpose of this study is to determine the technetium-99m pertechnetate (99mTcO4) intercellular uptake by different types of cell lines. HeLa, human fetal
osteoblast (hFOB), glial and glioma cell lines grown in 6-wells culture plates were incubated with 99mTcO4 of activity of 200, 400, 600. 800 and 1000
pCi for 30 minutes at 3T°C and 5% ( '< - humidified atmosphere. After incubation, the cells were washed 3 times with phosphate buffer saline to remove the
extracellular traces of 99mTcO4. Measurements of the intercellular Q9mTcOjt radioactivity were performed using single head gamma
camera and the percentage uptake of the yUwTcGpinto the cells was calculated. The intercellular uptake
0fyUmTcO_( was found to be inversely correlate to the radioactivity HeLa cell shows the highest uptake
followed by hFOB, glial and glioma cell lines. Comparison of uptake between normal and cancer cells
present indistinguishable results. The findings of this study suggest that the intercellular uptake of
yymTcOjt is highly dependent on the type of cells despite no significant different of uptake was found
between normal and cancer cell lines. The level of radioactivity is also an important determinant
factor that influence the uptake ofyUmTcG) into the cells. This study will be the first precedent toward
understanding the cellular characteristic and pharmacokinetic of non-invasive imaging tracer for
future molecular imaging and therapy.
Fulltext Optimization of food waste utilization in the RDF recovery energy facility

Abdul Rashid, Z., Alias, A.B., Ku Hamid, K.H., Wan Abdul, K. G.

International Food Research Journal, 2016;23(101):37-43.
MyJurnal

It is well known that there is a significant increased in the quantity of Municipal Solid Waste
(MSW) generated every year. Therefore, the management of MSW must be handled carefully
by experts. Malaysia as one of the developing country with population nearly 30 millions
also facing problems related to MSW management and this issue is quite critical. Even though
various programmes were established and few model technologies were introduced by the
government to manage MSW effectively, but the impact remain unsatisfaction. For example,
the energy facility recovery project which use MSW gathered around Kajang as an alternative
fuel still not successful to provide energy according to the design capacity of that particular
facility. This failure was observed due to the impact composition of MSW collected which
consists huge amount of food waste (57%), 16% plastic, 15% diaper, 9% mixed papers and
the remaining 3% textile. The existing process technology at Refused Derived Fuel (RDF)
facility is no designed to create a new economic benefit from abundant incoming food waste
to the facility. Therefore in this paper, the analysis and suggestion of technology on existing
RDF recovery energy facility will be discussed especially on how to overcome the abundant
composition of food waste.
Fulltext Optical and Photoacoustic Properties of Laser-Ablated Silver Nanoparticles in a Carbon Dots Solution

Sadrolhosseini AR, Krishnan G, Shafie S, Abdul Rashid S, Wadi Harun S

Molecules, 2020 Dec 09;25(24).
PMID: 33316885 DOI: 10.3390/molecules25245798

This study used the carbon dots solution for the laser ablation technique to fabricate silver nanoparticles. The ablation time range was from 5 min to 20 min. Analytical methods, including Fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy, transmission electron microscopy, and Raman spectroscopy were used to categorize the prepared samples. The UV-visible and z-scan techniques provided optical parameters such as linear and nonlinear refractive indices in the range of 1.56759 to 1.81288 and 7.3769 × 10-10 cm2 W-1 to 9.5269 × 10-10 cm2 W-1 and the nonlinear susceptibility was measured in the range of 5.46 × 10-8 to 6.97 × 10-8 esu. The thermal effusivity of prepared samples, which were measured using the photoacoustic technique, were in the range of 0.0941 W s1/2 cm-2 K-1 to 0.8491 W s1/2 cm-2 K-1. The interaction of the prepared sample with fluoride was investigated using a Raman spectrometer. Consequently, the intensity of the Raman signal decreased with the increasing concentration of fluoride, and the detection limit is about 0.1 ppm.
Effect of the Novel Biodegradable N, O-Carboxymethylchitosan and Oligo-Chitosan on the Platelet Thrombogenicity Cascade in von Willebrand Disease

Periayah MH, Halim AS, Mat Saad AZ, Yaacob NS, Hussein AR, Abdul Karim F, et al.

Thromb Res, 2015 Sep;136(3):625-33.
PMID: 26254703 DOI: 10.1016/j.thromres.2015.07.027

Von Willebrand disease (vWD) is the second least common hemostatic disorder in Malaysia, and it has a low prevalence. This study examined the underlying platelet thrombogenicity cascades in the presence of different formulations of chitosan-derivatives in vWD patients. This paper aimed to determine the significant influence of chitosan biomaterial in stimulating the platelet thrombogenicity cascades that involve the von Willebrand factor, Factor 8, Thromboxane A2, P2Y12 and Glycoprotein IIb/IIIa in vWD.

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