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Fulltext Outcome of Skeletal Reconstructive Surgery for Metastatic Bone Tumours in the Femur

Mohamed-Haflah NH, Kassim Y, Zuchri I, Zulmi W

Malays Orthop J, 2017 Mar;11(1):28-34.
PMID: 28435571 MyJurnal DOI: 10.5704/MOJ.1703.013

INTRODUCTION: The role of surgery in skeletal metastasis is to reduce morbidity and improve the quality of life in terminally ill patients. We report our experience with patients who underwent skeletal reconstructive surgery for metastatic bone tumour of the femur.
MATERIALS AND METHODS: Twenty nine operations for skeletal metastasis of the femur performed in our centre between 2009 and 2015 were included in this study. We evaluated the choice of implant, complications, survival rate and functional outcome. Fourteen patients were still alive at the time of this report for assessment of functional outcome using Musculoskeletal Tumour Society (MSTS) form.
RESULTS: Plating osteosynthesis with augmented-bone cement was the most common surgical procedure (17 patients) performed followed by arthroplasty (10 patients) and intramedullary nailing (2 patients) There were a total of five complications which were implant failures (2 patients), surgical site infection (2 patients), and site infection mortality (1 patient). The median survival rate was eight months. For the functional outcome, the mean MSTS score was 66%.
CONCLUSION: Patients with skeletal metastasis may have prolonged survival and should undergo skeletal reconstruction to reduce morbidity and improve quality of life. The surgical construct should be stable and outlast the patient to avoid further surgery.
Combating chlorhexidine allergy in perioperative setting

Shamsher S, Suhaimi NHI, Noor Ali R, Zulkifli K

Med J Malaysia, 2023 Jan;78(1):126-127.
PMID: 36715203

Chlorhexidine is labelled as hidden allergen as the health care professionals (HCPs) are unaware of the wide range of products containing chlorhexidine. Adverse events from chlorhexidine allergy can be reduced by appropriate perioperative management especially heeding on positive history during preoperative assessment, awareness regarding this hidden allergen, and educating HCPs on possible chlorhexidine-containing products. The regulatory agencies all over the world have issued recommendations regarding safety and risk of hypersensitivity reactions with chlorhexidine-containing products. The onus lies on HCPs to disseminate this knowledge to the stakeholders. We present a brief update to combat chlorhexidine allergy in perioperative setting.
Fulltext Fixatives increase the efficacy of gel formulations containing piper aduncum linnaeus (piperales: piperaceae) essential oil as repellenty

Mamood, S. N. H., Budin, S. B., Ahmad Rohi, G., Hidayatulfathi, O., Kalaivany, M., Zulfakar, M. H.

Jurnal Sains Kesihatan Malaysia, 2019;17(1):9-15.
MyJurnal

Piper aduncum essential oil exhibit repellency activity and has a potential to be use as an alternative for synthetic repellent such as N,N-diethyl-3- methylbenzamide, (DEET). However, the volatility properties of the essential oil decrease their persistence as a topical repellent. Study has shown that formulation of the essential oil with some fixatives may increase their effectiveness. Therefore, this study was conduct to evaluate the effectiveness of gel formulation containing P. aduncum essential oil with two fixative; vanillin and paraffin oil. Gel formulations containing P. aduncum essential oil with 5% and 10% vanillin and 6% paraffin oil was prepared and tested against Aedes aegypti in laboratory using Standards and Industrial Research Institute of Malaysia (SIRIM) bioassay method. After 240 minute post-application, formulation containing 5% and 10% vanillin was able to provide >70% repellency percentage against mosquito while formulation containing 6% paraffin oil gives
Fulltext Application of seaweed (Kappaphycus alvarezii) in Malaysian food products

Mohammad, S. M., Mohd Razali, S. F., Mohamad Rozaiman, N. H. N., Laizani, A. N., Zawawi, N.

International Food Research Journal, 2019;26(6):1677-1687.
MyJurnal

Kappaphycus alvarezii is a species of red algae, and one of the most important carrageenan
sources for food, cosmetic, and pharmaceutical industries. It is commercially cultivated in the
eastern part of Malaysia. Although K. alvarezii is rich in nutrients, it is limited in its integration
into Malaysian food products. Therefore, the present work was conducted to investigate the
quality characteristics, sensorial attributes, and antioxidant activity of K. alvarezii in Malaysian
food products. Seaweed puree (SP) from K. alvarezii at 10%, 20% and 30% concentrations were
prepared in the formulations of fish sausages, flat rice noodles and yellow alkaline noodles.
Proximate analysis, physicochemical analysis, microbial count, total phenolic content (TPC),
sensory evaluation, and consumer acceptance survey of the formulated food were conducted.
The incorporation of K. alvarezii significantly increased the fibre, moisture, and ash content in
formulated foods. In addition, the TPC content of K. alvarezii food also significantly increased
up to 42 mg GAE/100 g. The presence of SP in food at higher concentration decreased the
microbial counts. Sensory analysis confirmed that only fish sausages added with SP was
overall acceptable as compared to control. Based on customer survey, functional foods that are
“research proven” were the most preferred. In conclusion, K. alvarezii has the potential to be
incorporated in Malaysian food products and developed as functional food.
Fulltext Semiquantitative screening test for G6PD deficiency detects severe deficiency but misses a substantial proportion of partially-deficient females

Ainoon O, Alawiyah A, Yu YH, Cheong SK, Hamidah NH, Boo NY, et al.

Southeast Asian J. Trop. Med. Public Health, 2003 Jun;34(2):405-14.
PMID: 12971572

Neonatal screening for G6PD deficiency has long been established in many countries. The aim of the study was to determine whether the routine semiquantitative fluorescent spot test could detect all cases of G6PD deficiency, including those cases with partial deficiency (residual red cell G6PD activity between 20-60% of normal). We compared the results of G6PD screening by the semiquantitative fluorescent spot test and quantitative G6PD activity assay on a group of 976 neonates and 67 known female heterozygotes. The values for mean G6PD activity of G6PD-normal neonates and 293 healthy adult females were determined. There was no significant difference in the mean normal G6PD activity between the two racial groups in the neonates (669 Malays, 307 Chinese) and in the 293 healthy adult females (150 Malays, 143 Chinese) group. The values for the upper limits of total deficiency (20% of normal residual activity) for neonates and adult females were 2.92 U/gHb and 1.54 U/gHb, respectively. The upper limits of partial deficiency (60% of normal residual activity) were 8.7 U/gHb and 4.6 U/gHb respectively. The prevalence of G6PD deficiency among the male neonates was 5.1% (26) by both the fluorescent spot test and the enzyme assay method. The G6PD activity levels of all 26 cases of G6PD-deficient male neonates were < 20% normal (severe enzyme deficiency). In the female neonate group, the frequency of G6PD deficiency was 1.3% (6 of 472) by the fluorescent spot test and 9.35% (44 of 472) by enzyme assay. The 6 cases diagnosed as deficient by the fluorescent spot test showed severe enzyme deficiency (< 2.92 U/gHb). The remaining 38 female neonates had partial enzyme deficiency and all were misdiagnosed as normal by the fluorescent spot test. In the female heterozygote group, G6PD deficiency was diagnosed in 53% (35 of 67) by enzyme assay and in 7.5% (4 of 67) of cases by the fluorescent spot test. The 4 cases detected by fluorescent spot test had severe enzyme deficiency (<1.6 U/gHb). The remaining 31 (46.3%) cases, diagnosed as normal by fluorescent spot test, showed partial G6PD deficiency. In conclusion, we found that the semiquantitative fluorescent spot test could only diagnose cases of total G6PD deficiency and misclassified the partially-deficient cases as normal. In this study, the overall prevalence of G6PD deficiency was 3.28% by the semiquantitative fluorescent spot test and 7.17% by enzyme assay. This means that 3.9% of G6PD-deficient neonates were missed by the routine fluorescent spot test and they were found to be exclusively females. This study demonstrates a need to use a method that can correctly classify female heterozygotes with partial G6PD deficiency. The clinical implication is that these individuals may be at risk of the hemolytic complication of G6PD deficiency.
Atrovirinone inhibits proinflammatory mediator synthesis through disruption of NF-kappaB nuclear translocation and MAPK phosphorylation in the murine monocytic macrophage RAW 264.7

Israf DA, Tham CL, Syahida A, Lajis NH, Sulaiman MR, Mohamad AS, et al.

Phytomedicine, 2010 Aug;17(10):732-9.
PMID: 20378317 DOI: 10.1016/j.phymed.2010.02.006

In a previous communication we showed that atrovirinone, a 1,4-benzoquinone isolated from the roots of Garcinia atroviridis, was able to inhibit several major proinflammatory mediators of inflammation. In this report we show that atrovirinone inhibits NO and PGE(2) synthesis through inhibition of iNOS and COX-2 expression. We also show that atrovirinone inhibits the secretion of IL-1beta and IL-6 in a dose dependent fashion whereas the secretion of IL-10, the anti-inflammatory cytokine, was enhanced. Subsequently we determined that the inhibition of proinflammatory cytokine synthesis and inducible enzyme expression was due to a dose-dependent inhibition of phosphorylation of p38 and ERK1/2. We also showed that atrovirinone prevented phosphorylation of I-kappaBalpha, which resulted in a reduction of p65NF-kappaB nuclear translocation as demonstrated by expression analysis. We conclude that atrovirinone is a potential anti-inflammatory drug lead that targets both the MAPK and NF-kappaB pathway.
A prospective study on the pattern of medication use for schizophrenia in the outpatient pharmacy department, Hospital Tengku Ampuan Rahimah, Selangor, Malaysia

Ponto T, Ismail NI, Abdul Majeed AB, Marmaya NH, Zakaria ZA

Methods Find Exp Clin Pharmacol, 2010 Jul-Aug;32(6):427-32.
PMID: 20852752 DOI: 10.1358/mf.2010.32.6.1477907

Schizophrenia is a chronic psychiatric disorder and pharmacotherapy plays a major role in its management. The 1950s and early 1960s saw milestones in the introduction of psychotropic drugs in clinical practice. A review of drug prescriptions in different settings provides an insight into the pattern of drug use, identifies drug-related problems and may be used to compare recommended guidelines with actual practice. This effort led to the evaluation of the drug prescribing pattern of antipsychotics in patients attending the psychiatric clinic at a government hospital. The data from 371 antipsychotic medication prescriptions that included 200 prescriptions for schizophrenia were collected during one month (1rst-31rst August 2008) at the outpatient pharmacy department. The mean age of patients was 35.0 years (SD = 1.131), with a male to female ratio of 2:1. The most widely used oral antipsychotic was haloperidol (16.3%) while the most common depot preparation prescribed was zuclopenthixol decanoate (8.8%). The daily dose of the average antipsychotic prescribed in this clinic was 342.06 mg equivalent of chlorpromazine. There was no relation between the doses received and ethnicity of the patient (Malay, Chinese or Indian). However, there was a significant relationship between the prescribed dose and patient age (P < 0.042). Nearly 32% of the schizophrenia patients were prescribed with atypical antipsychotics such as olanzapine (10.8%), risperidone (10.0%), quetiapine (7.6%) and clozapine (3.2%). Monotherapy was given to 73.0% of the schizophrenia patients. The majority of patients also received antidepressants. To conclude, this study gave evidence that physicians had a strong preference for monotherapy with conventional antipsychotic drugs while the use of atypical drugs was less prevalent.
Fulltext Lightning performance analysis of a rooftop grid-connected solar photovoltaic without external lightning protection system

Nasir MSM, Ab-Kadir MZA, Radzi MAM, Izadi M, Ahmad NI, Zaini NH

PLoS One, 2019;14(7):e0219326.
PMID: 31295278 DOI: 10.1371/journal.pone.0219326

The Sustainable Energy Development Authority of Malaysia (SEDA) regularly receives complaints about damaged components and distribution boards of PV systems due to lightning strikes. Permanent and momentary interruptions of distribution circuits may also occur from the disturbance. In this paper, a solar PV Rooftop system (3.91 kWp) provided by SEDA was modelled in the PSCAD/EMTDC. The Heidler function was used as a lightning current waveform model to analyse the transient current and voltage at two different points susceptible to the influence of lightning events such as different lightning current wave shape, standard lightning current and non-standard lightning current. This study examines the effect on the system components when lightning directly strikes at two different points of the installation. The two points lie between the inverter and the solar PV array and between inverter and grid. Exceptionally high current and voltage due to the direct lightning strike on a certain point of a PV Rooftop system was also studied. The result of this case study is observed with and without the inclusion of surge protective devices (SPDs). The parameters used were 31 kA of peak current, 10 metres cable length and lightning impulse current wave shape of 8/20μs. The high current and voltage at P1 striking point were 31 kA and 2397 kV, respectively. As for the AC part, the current and voltage values were found to be 5.97 kA and 5392 kV, respectively.Therefore, SPDs with suitable rating provided by SEDA were deployed. Results showed that high transient current voltage is expected to clamp sharply at the values of 1.915 kV and 0 A at the P1 striking point. As for the AC part, the current and voltage values were found to be 0 kA and 0.751 V, respectively. Varying lightning impulse current wave shapes at striking point P2 showed that the highest voltage was obtained at waveshape 10/350 μs at 11277 kV followed by wave shapes of 2/70 μs, 8/20 μs and 0.7/6 μs. The high value of transient voltage was clamped at a lower level of 2.029 kV. Different lightning amplitudes were also applied, ranging from 2-200 kA selected based on the CIGRE distribution. It showed that the current and voltage at P1 and P2 were directly proportional. Therefore, the SPD will be designed at an acceptable rating and proper position of SPD installation at solar PV Rooftop will be proposed. The results obtained in this study can then be utilised to appropriately assign a SPD to protect the PV systems that are connected to the grid. Installing SPDs without considering the needs of lightning protection zones would expose the expensive equipment to potential damage even though the proper energy coordination of SPDs is in place. As such, the simulation results provide a basis for controlling the impacts of direct lightning strikes on electrical equipment and power grids and thus justify SPD coordination to ensure the reliability of the system.
Influence of various Cu/Fe ratios on the surface properties of green synthesized Cu-Fe-BTC and it`s relation to methylene blue adsorption

Siew WY, Abu Bakar NHH, Abu Bakar M, Zainal Abidin A

J Hazard Mater, 2021 Aug 15;416:125846.
PMID: 34492799 DOI: 10.1016/j.jhazmat.2021.125846

The incorporation of different percentages of Fe2+ into copper benzene-1,3,5-tricarboxylate (CuBTC) was successfully carried out at room-temperature synthesis with water as the only solvent. The XRD and XPS analysis shows that the Fe2+ were substituted into the paddlewheel structure. The incorporation of 18% Fe2+ into CuBTC can increase the surface area and porosity of the framework. The BET surface area of Cu82Fe18BTC (1240 m2/g) was significantly higher than CuBTC (708 m2/g). Further increase in the Fe2+ percentage will reduce the surface area of the compound. The presence of Fe2+ in the framework successfully disturbs the pore formation and widens the pore size on the surface of these compounds. This as well as the pHpzc, which is related to the surface acidity of the resulting bimetallic organic framework (BMOF), play an important role in the adsorption process. Cu53Fe47BTC with an adsorption capacity of 94.42 mg/g shows approximately 6 times greater adsorption capacity against MB compared to CuBTC. This shows that by utilizing a different ratio of Cu and a second metal, it is possible to effectively design the surface morphology of BMOF for specific applications.
Relationship between reproductive exposures and age-related cataract in women

Noran NH, Salleh N, Zahari M

Asia Pac J Public Health, 2007;19(2):23-8.
PMID: 18050560

The objective of this study is to evaluate the relationship between reproductive exposures and age-related cataract among women. This was a hospital based case-control study. The study population included female patients, aged 50 years and above who attended the Eye clinic at the University of Malaya Medical Centre. The outcome measurement was based on ophthalmologic examination by an ophthalmologist. The data on exposure was obtained from face to face interview using a structured questionnaire. In order to reduce the recall bias, patients' medical records were used to substantiate the exposure status. Multiple logistic regression was used to assess the association of age-related cataract with exogenous estrogen usage (HRT and OCP) and duration of menses. Important confounders such as age, history of diabetes, cigarette smoking and steroids usage were controlled for in the analysis. Females with 29 years or less of endogenous estrogen exposure of, have almost three times the risk of developing age related cataract (adjusted OR 3.42: 95% CI: 1.28, 9.16), similarly among those with exposure of 30-32 years (adjusted OR 3.64: 95% CI: 1.08, 12.26). Hormone Replacement Therapy used for more than three years was found to be a protective factor of age-related cataract. There is evidence that reproductive exposure may play a role in reducing the occurrence of age-related cataract among Malaysian women.
Study site: Eye clinic, University Malaya Medical Centre (UMMC), Kuala Lumpur, Malaysia
Fulltext Exposure Level to Caregivers Resulting From the Scattered Radiation During a Radiographic Procedure

Sayed, I. S., Yusri, N. H.

Physics and Technology in Medicine, 2020;1(1):22-26.
MyJurnal

In radiography, radiation workers are responsible to protect patients and their caregivers from adverse effects of X-rays during diagnostic procedures. The X-ray examination rooms are designated as controlled areas where only authorised persons are allowed to enter. However, sometimes radiographers allow next in-line patients’ and caregivers in X-ray examination room and ask them to stand behind the mobile lead shielding when exposure is on. The objectives of this study were to determine the amount of scatter radiation dose at different heights with respect to the floor in the X-ray examination room and to educate and increase the awareness of radiation workers about the scattered radiation in minimizing the unnecessary radiation dose to patient’s caregivers. Siemens Multix Top X-ray system was used. Kyoto Kagaku PBU-50 whole body phantom was scanned. The phantom (torso) was positioned for anteroposterior (AP) lumbar projection on the examination table. The nanoDot OSLDs were fixed behind the lead shielding at different heights (120, 130, 140, 150, 160 and 170 cm) with respect to the floor 2.5 meters away from the central ray of X-ray beam. The phantom was exposed using different tube voltages 68 kVp, 79 kVp and 90 kVp at a constant tube current of 32 mAs fixing a 100 cm source to image distance (SID). Scatter radiation doses measured at different heights were different for each exposure. The highest scattered radiation dose measured was 6.4 mGy at 130 cm height for 79 kVp exposure. In conclusion the measured scattered radiation doses were within the acceptable annual dose limits as recommended by NCRP 116 and ICRP 103 for patient caregiver. However, a smallest amount of radiation dose may increase the risk of cancer. Thus, the negligence must not be overlooked because it exposes the caregiver to unnecessary radiation.
Fulltext Measurement and Mapping of Wi-Fi Radiation Level at Hamdan Tahir Library, Universiti Sains Malaysia Health Campus

Supardi, N. F., Mohd Taib, N. H., Abu Amat, N. H., Yusoff, M. N. S.

Physics and Technology in Medicine, 2020;1(1):52-57.
MyJurnal

Wi-Fi is a wireless communication technology that uses specific electromagnetic frequencies. The increasing use of Wi-Fi has raised public concerns about the impact of electromagnetic radiation on the environment and human health. Since the exposure level of the electromagnetic field (EMF) radiation differs between different locations, it is important to measure the strength of the EMF at various locations under observation. This study aimed to obtain specific values related to the radiofrequency and microwave EMF which is described by four specific parameters, that are 1) the frequency of the wave, 2) the electric field strength E, 3) the magnetic field strength H, and 4) the power density S. This study was carried out at the first floor area of Hamdan Tahir Library, Universiti Sains Malaysia Health Campus. Mapping of Wi-Fi signal and measurement of Wi-Fi radiation level was performed at four specific locations, that are Laptop zone 1, Laptop zone 2, Computer lab, and Cozy corner. The average radiation level was compared with the ICNIRP standard limit for public user. It was observed that the Wi-Fi signal was highest in Laptop zone 2 followed by Laptop zone 1 which displayed a moderate signal strength. Whereas moderate but lower signal level was detected in Computer lab zone and Cozy corner. The electric and magnetic fields as well as power density were found highest in Laptop zone 1, followed by Laptop zone 2, Cozy corner, and Computer lab. Comparison with standard ICNIRP limit showed that the radiation level is still far below the ICNIRP limit, which is only 2% of exposure level. To conclude, Laptop zone 2 exhibited the strongest Wi-Fi signal whereas Laptop zone 1 displayed the highest radiation level. However, the strength of the electric and magnetic fields as well as power density is still far below the ICNIRP limit.
Fulltext Measurement and Mapping of Wi-Fi Radiation Level at Students Hostel in Universiti Sains Malaysia Health Campus

Abu Amat, N. H., Mohd Taib, N. H., Supardi, N. F., Yusoff, M. N. S.

Physics and Technology in Medicine, 2020;1(1):38-44.
MyJurnal

The increasing use of wireless communication devices, particularly Wi-Fi has raised public concerns on the exposure to electromagnetic field (EMF) and its possible effect on human health. As the exposure level of the EMF radiation varies between different locations, measurement of the EMF strength at various locations is vital. In this study, we aimed to measure the EMF exposure which is described by four specific parameters, specifically 1) the frequency of the wave, 2) the electric field strength E, 3) the magnetic field strength H, and 4) the power density S. This study was performed at the second floor in Nurani hostel block in Desasiswa Murni Nurani, Universiti Sains Malaysia Health Campus. Mapping of Wi-Fi signal and measurement of Wi-Fi radiation level was performed at four specific locations, that are in a student room, television room, prayer room, and ironing room. The average radiation level was compared with the standard limit set by International Commission on Non-Ionizing Radiation Protection (ICNIRP). It was observed that the strength of Wi-Fi signal was highest in students’ room followed by television room. Both of these rooms exhibited high signal strength. While moderate but lower signal level was observed in prayer room followed by ironing room. The electromagnetic field and power density were found highest in students’ room, followed by television room, prayer room, and ironing room. Comparison with standard ICNIRP limit showed that the radiation level is still far below the acceptable limit, which is only 2% of the exposure level. To conclude, students’ room exhibited the strongest Wi-Fi signal and the highest radiation level. However, the radiation level especially power density is still far below the ICNIRP limit.
Fulltext Anti-cancer potential of pseuduvaria macrophylla in human cancer cell lines

Taha, H., Mohebali, N., Chung, Y. L., Ismail, N. H., Aziz , A., Bakar, M. Z. A., et al.

Journal of Advanced Research in Applied Sciences and Engineering Technology, 2016;4(1):1-11.
MyJurnal

his is a short report on the recent investigation on the anticancer and antioxidant potential
of Pseuduvaria macrophylla stem bark and leaf extract on human breast cancer cell (MCF-7), human
prostate cancer cell (PC-3) and human colon cancer cell (HT29). LCMS experiment was done to
identify the chemical composition in the methanolic extracts while GCMS analysis was done to
determine the chemical composition in the hexanolic extracts. For antioxidant activity, the most active
extract was leaf methanolic with 69.22 % of DPPH inhibition. Additionally, the leaf methanolic extract
inhibited the better proliferation especially on human breast cancer cell (MCF-7) compared to hexane
crude extracts due to more than 90% of cancer cell death and lowest IC50 value at range of 80.70
µg/mL ± 0.08 – 106.70 µg/mL ± 0.07. The major detected compounds were α-Cadinol, neophytadiene,
palmitic acid, linoleic acid, oleic acid and isopolycerasoidal and isopolycerasoidal methyl ester. The
findings of this study support the anti-cancer claims of Pseuduvaria macrophylla leaf. Copyright© 2016
Penerbit Akademia Baru- All rights reserved.
Evaluation and application of a simple and rapid method for the analysis of aflatoxins in commercial foods from Malaysia and the Philippines

Ali N, Hashim NH, Yoshizawa T

Food Addit Contam, 1999 Jul;16(7):273-80.
PMID: 10656052

For application to the analysis of aflatoxins (AF) in commercial peanut and corn products, the ISOLUTE multimode column (IMC, solid phase multifunctional column) method was validated by comparing with the modified Florisil column (MFC) method. Twenty-two peanut and eight corn products from Malaysia and the Philippines were analysed for AFB1, AFB2, AFG1 and AFG2 firstly by the MFC method and then by the IMC method. For peanut products, 14 out of 22 samples were positive by the two methods in the range of 1-378 micrograms/kg of AF, and correlation coefficients (r) for AFB1 and AFB2 were 0.987 and 0.997, respectively. For corn and corn products, all the samples were positive in the range of 1-130 micrograms/kg, and r values were 0.992 and 0.805 for AFB1 and AFB2 respectively. Thus, the results were significantly (p < 0.01) in close agreement, particularly for lower range of 1-50 micrograms/kg of AF concentrations in all the samples. For the occurrence of AF, 11 (65%) of peanut products from Malaysia were contaminated with AF at a mean level of 50 micrograms/kg (maximum 180 micrograms/kg) and two (40% products from the Philippines were contaminated with as high as 375 micrograms/kg and 177 micrograms/kg of AF, respectively. All the corn products from the Philippines were contaminated with AF at a mean level of 44 micrograms/kg (maximum 130 micrograms/kg). Contamination of commercial foods with high levels of AF is a very important issue to both the countries since these foods are very popular among children.
Evaluation of a method to determine the natural occurrence of aflatoxins in commercial traditional herbal medicines from Malaysia and Indonesia

Ali N, Hashim NH, Saad B, Safan K, Nakajima M, Yoshizawa T

Food Chem Toxicol, 2005 Dec;43(12):1763-72.
PMID: 16019122

Traditional herbal medicines, popularly known as 'jamu' and 'makjun' in Malaysia and Indonesia, are consumed regularly to promote health. In consideration of their frequent and prolonged consumption, the natural occurrence of aflatoxins (AF) in these products was determined using immunoaffinity column clean-up and high-performance liquid chromatography with pre-column derivatization. The evaluated method, which entails dilution of sample extracts with Tween 20-phosphate buffered saline (1:9, v/v) and a chromatographic system using isocratic mobile phase composed of water-methanol-acetonitrile (70:20:10, v/v/v), was effective in separating AFB1, AFG1 and AFG2 from interference at their retention times. Results were confirmed using post-column derivatization with photochemical reactor. For 23 commercial samples analyzed, mean levels (incidence) of AFB(1), AFB(2) and AFG1 in positive samples were 0.26 (70%), 0.07 (61%) and 0.10 (30%) microg/kg, respectively; one sample was positive for AFG2 at a level of 0.03 (4%) microg/kg. In contrast to the high levels of AF in crude herbal drugs and medicinal plants reported previously by other researchers, the low contamination levels reported in this study may be attributed to the higher selectivity to AF of the method applied. Based on the AFB1 levels and the daily consumption of positive samples, a mean probable daily intake of 0.022 ng/kg body weight was calculated.
The edema inducing activity of phospholipase A2 enzymes

Tan NH, Saifuddin MN, Yong WY

Biochem. Int., 1991 Jan;23(1):175-81.
PMID: 1863271

The edema inducing activity of phospholipase A2 (PLA2) enzymes from snake venoms and porcine pancreas was investigated using mouse paw as experimental model. All ten PLA2 enzymes exhibited potent edema inducing activity. PLA2, however, is generally not the major edema inducing component of snake venom. Chemical modification studies indicated that enzymatic activity of PLA2 was required for its edema inducing activity. All PLA2 enzymes examined displayed a rapid onset edema which was suppressed by pretreatment of the mice with antihistamine. Dexamethasone pretreatment also inhibited edemas elicited by some PLA2 enzymes.
Fulltext Functional Outcome of Surgical Stabilisation of Acetabular Fractures

Fakru NH, Faisham WI, Hadizie D, Yahaya S

Malays Orthop J, 2021 Jul;15(2):129-135.
PMID: 34429833 DOI: 10.5704/MOJ.2107.019

Introduction: Approach to the management of displaced acetabular fractures has evolved from conservative to operative management after the work of Judet and Letournel. Various surgical methods have been explored and described by authors to address this type of fracture, leading to improved clinical outcome. This study aimed to evaluate functional outcome of surgically treated displaced acetabular fractures in the Malaysian context.
Materials and methods: We analysed 43 patients with isolated acetabular fractures who were treated operatively with a minimum of three years follow-up. Anthropometric data, Judet-Letournel fracture pattern, surgical approach and complications were recorded. Post-operative Matta radiological outcome were evaluated for joint congruency and hip functional outcome was evaluated using Merle d'Aubgine-Postel and Harris Hip Score (HHS). All statistical analyses were analysed using SPSS version 24.0.
Results: The most frequent elementary fracture type was posterior wall (30.2%) while associated type was both columns (23.3%). Mean functional outcome of Merle d'Aubigné-Postel was 15.77 and HHS was 86.6. Thirty-three (76.7%) patients achieved satisfactory functional outcome, 19 (44.1%) patients achieved anatomic reduction (<2 mm step-off) based on Matta classification while 24 (55.8%) did not achieve the desired outcome. Fracture pattern exhibited strong association with post-operative Matta radiological outcome (p-value 0.001). However both fracture pattern and Matta radiological outcome did not exhibit association with the functional outcome group. The mean time for surgical interventions was 10.8 days and there was no significant association with final functional outcome score.
Conclusion: Fracture pattern is a strong contributing factor towards post-operative Matta radiological outcome. However, achieving the perfect anatomical reduction is not of utmost important factor to predict the good functional outcome.
Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA

Rusul G, Yaacob NH

Int J Food Microbiol, 1995 Apr;25(2):131-9.
PMID: 7547144

Enterotoxigenic Bacillus cereus was detected in cooked foods (17), rice noodles (3), wet wheat noodles (2), dry wheat noodles (10), spices (8), grains (4), legumes (11) and legume products (3). One hundred ninety-four (42.3%), 70 (15.3%) and 23 (5.2%) of the 459 presumptive B. cereus colonies isolated from PEMBA agar were identified as B. cereus, Bacillus thuringiensis and B. mycoides, respectively. B. cereus isolates were examined for growth temperature, pH profile and enterotoxin production using both TECRA-VIA and BCET-RPLA kits. One hundred seventy-eight (91.8%) and 164 (84%) of the strains were enterotoxigenic as determined using TECRA-VIA and BCET-RPLA, respectively. Eighty-two (50%) of the enterotoxigenic strains were capable of growing at 5 degrees C, and 142 (86.6%) grew at 7 degrees C within 7 days of incubation. The enterotoxigenic strains did not grow at pH 4.0 but 69 (42.0%) of the strains were able to grow at pH 4.5 within 7 days at 37 degrees C. The isolates were resistant to ampicillin (98.8%), cloxallin (100%) and tetracycline (61.0%), and susceptible to chloroamphenicol (87%), erythromycin (77.4%), gentamycin (100%) and streptomycin (98.7%).
Electrophoretic profiles and biological activities: intraspecific variation in the venom of the Malayan pit viper (Calloselasma rhodostoma)

Daltry JC, Ponnudurai G, Shin CK, Tan NH, Thorpe RS, Wüster W

Toxicon, 1996 Jan;34(1):67-79.
PMID: 8835335

The Malayan pit viper (Calloselasma rhodostoma) is of major clinical significance both as a leading cause of snakebite and as the source of ancrod (Arvin). Although its venom has been extensively studied, the degree to which venom composition varies between individuals is poorly known. We individually analysed the venoms of over 100 C. rhodostoma using isoelectric focusing. In all populations, females produced an intense band that was absent from all males, and significant ontogenetic variation was detected. Principal components analysis of the banding profiles also revealed strong geographic variation, which was significantly congruent with variation in the biological activities of the venom (phosphodiesterase, alkalinephosphoesterase, L-amino acid oxidase, arginine ester hydrolase, 5'-nucleotidase, thrombin-like enzyme, haemorrhagic activity). Studies of captive-bred snakes indicate that the intraspecific variation in venom is genetically inherited rather than environmentally induced. The intraspecific variation in venom composition and biological activity could be of applied importance to snakebite therapy, both in correct diagnosis of the source of envenomation and in the development of a more effective antivenom. Greater attention should be given to the source of C. rhodostoma venom used in research to ensure reproducibility of results.

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