METHODS: Sixty-five cylindrical block of Fuji IX Fast were prepared using split moulds. The demineralizing solution was an acetate buffered demineralizing solution at pH 403. The remineralizing solution was a buffered solution containing 1.5 mM Ca, 0.9 mM P and 10 ppm F at pH 7. The blocks of Fuji IX Fast were subjected either to two-day alternating cycles of remineralization and demineralization for up to 24 days (test); 6 two-day cycles of demineralizing or remineralizing solution separately, or deionized distilled water alone (controls) or were left untreated (base line control). Mineral profiles of Ca, P, Sr and F within 100 microm of the material surface were assessed following 8, 16 and 24 days of treatment (test); 4, 8 or 12 days (controls) or for baseline control samples, using electron probe microanalysis (EPMA).
RESULTS: There were significant changes in mineral profile in the test specimens in terms of Sr and Ca concentrations. A molecule for molecule exchange of these elements resulted between GIC and eluant solutions. Fluoride loss from the GIC occurredto the level comparable with uptake levels recorded in eluant solutions from previous studies. The ionic exchanges appeared to be the result of dissolution followed by an equilibrium-driven diffusion. These exchanges were superficial though substantial.
CONCLUSIONS: Simulated exposure of Fuji IX to the oral environment resulted in an exchange of Ca from the bathing solutions into Fuji IX to replace any Sr which was lost to the GIC. Fluorine loss from the GIC followed previously described patterns. The possible clinical significance of this exchange was discussed.
Materials and Methods: Cell viability and cytotoxicity of gelatin (Gel; 50 µg/µl), chitosan (Chi; 20 µg/µl), hydroxyapatite (HA; 50 µg/µl), nanohydroxyapatite (nHA; 10 µg/µl), three-calcium phosphate (TCP; 50 µg/µl) and strontium carbonate (Sr; 10 µg/µl) were evaluated on hADSCs via MTT assay. In vivo femoral drill-bone hole model was produced in rats that were either left untreated or treated with autograft, Gel, Chi, HA, nHA, TCP and Sr, respectively. The animals were euthanized after 30 days. Their bone holes were evaluated by gross-pathology, histopathology, SEM and radiography. Also, their dry matter, bone ash and mineral density were measured.
Results: Both the Gel and Chi showed cytotoxicity, while nHA had no role on cytotoxicity and cell-viability. All the HA, TCP and Sr significantly improved cell viability when compared to controls (P<0.05). Both the Gel and Chi had no role on osteoconduction and osteoinduction. Compared to HA, nHA showed superior role in increasing new bone formation, mineral density and ash (P<0.05). In contrast to HA and nHA, both the TCP and Sr showed superior morphological, radiographical and biochemical properties on bone healing (P<0.05). TCP and Sr showed the most effective osteoconduction and osteoinduction, respectively. In the Sr group, the most mature type of osteons formed.
Conclusion: Various biomaterials have different in vivo efficacy during bone regeneration. TCP was found to be the best material for osteoconduction and Sr for osteoinduction.