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  1. Shahbazi P, Md Yusoff Musa, Tan AGY, Farhat Ahmadi Avin, Teo AWF, Sabaratnam V
    Sains Malaysiana, 2014;43:697-705.
    The isolation of 66 streptomycetes from rhizosphere soil of chili plants was done for their inhibitory activities against three different dominant species of Colletotrichum namely C. acutatum, C. gloeosporioides and C. capsici. Twenty one streptomycetes strains were active against at least one of the Colletotrichum species. In addition, ten strains that inhibited the in vitro growth of Colletotrichum species showed chitinase activity. Strain P42, which displayed the highest inhibitory activity against all three anthracnose fungi species and high chitinase activity was tested as biological control agent in a greenhouse study. The strain successfully controlled chili anthracnose disease by significantly reducing the disease severity. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain P42 belongs to the Streptomyces rochei clade. The results of the current study showed that rhizosphere-derived soil of chili plants is an important source of bioactive streptomycetes which are antagonistic against Colletotrichum.
  2. Ambayya, Angeli, Sasmita, Andrew Octavian, Zainina Seman, Chang, Kian Meng, Sathar, Jameela, Yegappan, Subramanian, et al.
    MyJurnal
    Insights into molecular karyotyping using comparative genomic hybridization (CGH) and single nucleotide polymorphism (SNP) arrays enable the identification of copy number variations (CNVs) at a higher resolution and facilitate the detection of copy neutral loss of heterozygosity (CN-LOH) otherwise undetectable by conventional cytogenetics. The applicability of a customised CGH+SNP 180K DNA microarray in the diagnostic evaluation of Acute Myeloid Leukaemia (AML) in comparison with conventional karyotyping was assessed in this study. Methods: Paired tumour and germline post induction (remission sample obtained from the same patient after induction) DNA were used to delineate germline variants in 41 AML samples and compared with the karyotype findings. Results: After comparing the tumour versus germline DNA, a total of 55 imbalances (n 5-10 MB = 21, n 10-20 MB = 8 and n >20 MB = 26) were identified. Gains were most common in chromosome 4 (26.7%) whereas losses were most frequent in chromosome 7 (28.6%) and X (25.0%). CN-LOH was mostly seen in chromosome 4 (75.0%). Comparison between array CGH+SNP and karyotyping revealed 20 cases were in excellent agreement and 13 cases did not concord whereas in 15 cases finding could not be confirmed as no karyotypes available. Conclusion: The use of a combined array CGH+SNP in this study enabled the detection of somatic and germline CNVs and CN-LOHs in AML. Array CGH+SNP accurately determined chromosomal breakpoints compared to conventional cytogenetics in relation to presence of CNVs and CN-LOHs.
  3. Mohamad Hsnul Bolhassan, Noorlidah Abdullah, Vikineswary Sabartnam, Hattori Tsutomu, Sumaiyah Abdullah, Noraswati Mohd Noor Rashid, et al.
    Sains Malaysiana, 2012;41:155-161.
    Macrofungi of the order Polyporales are among the most important wood decomposers and caused economic losses by decaying the wood in standing trees, logs and in sawn timber. Diversity and distribution of Polyporales in Peninsular Malaysia was investigated by collecting basidiocarps from trunks, branches, exposed roots and soil from six states (Johor, Kedah, Kelantan, Negeri Sembilan, Pahang and Selangor) in Peninsular Malaysia and Federal Territory Kuala Lumpur. This study showed that the diversity of Polyporales were less diverse than previously reported. The study identified 60 species from five families; Fomitopsidaceae, Ganodermataceae, Meruliaceae, Meripilaceae, and Polyporaceae. The common species of Polyporales collected were Fomitopsis feei, Amauroderma subrugosum, Ganoderma australe, Earliella scabrosa, Lentinus squarrosulus, Microporus xanthopus, Pycnoporus sanguineus and Trametes menziesii.
  4. Razak Wahab, Izyan Khalid, Tamer A. Tabet, Aminuddin Mohamed, Othman Sulaiman, Rafid MD. Salim, et al.
    Sains Malaysiana, 2012;41:163-169.
    The effectiveness of the hot oil treatment process on 15 year old cultivated Acacia hybrid was studied. Accelerated laboratory durability studies were conducted on the hot oil treated Acacia hybrid inoculated with fungi Coriolus versicolors, Gloeophyllum trabeum and Pycnoporus sanguineus. The logs of Acacia hybrid were harvested, segregated into bottom, middle and top portions, and later were oil-heat treated in an organic palm oil at temperatures of 180, 200 and 220°C for the duration of 30, 60 and 90 min. The wood samples that were dried and ground into sawdust was air-dried again before undergoing accelerated laboratory durability tests. Untreated samples were used as control. The durability of the wood increases with an increase in temperature and duration of the treatment. The hot oil treated samples could reduce the attack of G. trabeum from 20.89%, 20.94% and 21.29% in the control samples to 0.88-4.07%, 1.22-4.84% and 1.28-4.22% at bottom, middle and top portions, respectively. The attack of C. versicolors were reduced from 26.59%, 30.28% and 34.79% in the control samples to 2.89-9.41%, 3.88-16.84 and 4.27-17.34% at bottom, middle and top portions. However, the attacked of P. sanguineus were least effective with 31.42%, 36.33% and 36.55% in control samples to 3.26-12.55%, 4.67-15.36% and 4.69-19.22% at bottom, middle and top portions. Massive colonization of mycelia occurs in vessels of the untreated Acacia hybrid wood in comparison to the hot oil treated wood when observed through scanning electron microscope.
  5. Abdul Fattah Abu Bakar, Siti Nathasa Md Barkawi, Marlia Mohd. Hanafiah, Lee KE, Azhar Abdul Halim
    Sains Malaysiana, 2016;45:1509-1516.
    Keberkesanan rawatan air sisa industri automotif ditentukan dengan menggunakan kaedah penjerapan turus. Peratusan penyingkiran ammonia dan logam berat terpilih telah dikaji menggunakan pasir biasa dan pasir terubah suai secara kimia sebagai bahan penjerap. Dua model matematik iaitu Model Thomas serta Model Yoon-Nelson telah digunakan untuk menentukan kapasiti penjerapan maksimum ammonia. Peratusan penyingkiran ammonia menunjukkan pasir terubah suai secara kimia mencatatkan julat peratusan penyingkiran yang lebih tinggi iaitu 43.68% hingga 96.55% berbanding pasir biasa yang mencatatkan julat 0% hingga 89.66%. Logam berat zink, mangan, kromium, kuprum, arsenik, nikel, kobalt dan ferum mencatatkan peratusan penyingkiran antara 93% hingga 100% apabila menggunakan pasir terubah suai secara kimia manakala pasir biasa mencatatkan julat peratusan penyingkiran daripada 0.8% hingga 100%. Keputusan analisis menggunakan Model Thomas menunjukkan kapasiti penjerapan maksimum, qo ammonia menggunakan pasir terubah suai secara kimia (8.80 mg/g) adalah empat kali lebih tinggi daripada pasir biasa (2.57 mg/g) manakala masa bolos, t0.5 bahan penjerap yang ditentukan menggunakan Model Yoon dan Nelson mencatatkan masa tertinggi bagi pasir terubah suai secara kimia iaitu 30.18 min berbanding 9.57 min bagi pasir biasa. Kajian ini menunjukkan peratusan penyingkiran dan kapasiti penjerapan ammonia dan logam berat terpilih iaitu zink, mangan, kuprum, arsenik, nikel, kobalt dan ferum lebih tinggi bagi turus pasir terubah suai secara kimia berbanding pasir biasa.
  6. Najihan Abdul Samat Muttaqillah, Salasawati Hussin, Ainihayati Noordin, Chuan Hun Ding, Asrul Abdul Wahab, Md Mostafizur Rahman, et al.
    Sains Malaysiana, 2015;44:1315-1323.
    Multilocus sequence typing (MLST) has been used to characterise methicillin-resistant Staphylococcus aureus (MRSA) isolates into sequence types (STs) and together with SCCmec typing, form the clonal nomenclature for MRSA. MLST was conducted as per the standard protocol on ten out of 236 isolates collected previously from January to December 2009 representing four different SCCmec types. Relationship analysis was performed with eBURST via the MLST website. Four unlinked ‘singleton’ STs were detected: ST30, ST239, ST772 and ST1178. Together with SCCmec typing, five MRSA clones were identified: ST30-IV, ST239-II, ST239-III, ST772-V and ST1178-IV. Clones ST239-III and ST30-IV are already established in Malaysian hospitals and in the local community, respectively. ST772-V is an emerging clone reported previously to have a propensity to displace pre-existing predominant clones. A clone involving the predominant ST in Malaysia (ST239) with SCCmec type II is the first of its kind to be identified. MRSA clones in our centre are very diverse and clone surveillance with large sample sizes should be undertaken as collaborative efforts between local institutions to maximise detection coverage.
  7. Mohammad RK, Nadiah SM, Siti NF, Othman A, Khaidzir Hj I, Geshina Ayu MS
    Jurnal Psikologi Malaysia, 2014;olume 28:36-50.
    An individual’s personality traits seem to be a factor in developing and shaping violent criminal behaviour. Due to this realization, the present study seeks to explore the relationship of personality traits with low self-control, aggression, and self-serving cognitive distortion. The present study was an observational cross-sectional study using a guided self-administered questionnaire: M-PsychoQ. The source population was the male murderers incarcerated in 11 prisons in Peninsular Malaysia. 71 participants were selected based on a predetermined selection criteria using purposive sampling method. After undergoing the validation processes, the emerged M-PsychoQ consisted of four psychometric instruments: Malay versions of the Zuckerman Kuhlman Personality Questionnaire-40-Cross-Culture (ZKPQ-M-40-CC), Self-control Scale (SCS-M), Aggression Questionnaire (AQ-M-12), and “How I Think” Questionnaire (HIT-M). Regression analyses viz. simple and multiple linear regression approaches were conducted in order to predict whether personality traits would emerge as significant predictors. The findings evidenced certain personality traits as significant predictors of low self-control, high levels of aggression and self-serving cognitive distortion. This study imparts statistical evidence on the role of personality traits as an important predictor of low self-control, high aggression and self-serving cognitive distortion. The results were discussed in relation to the theory and context of murder.
  8. Noraidah Sahari @ Ashaari, Hairulliza Mohd Judi, Abdul Azim Abdul Ghani, Mohd Hasan Selamat, Aida Suraya Md Yunus
    Tujuan utama kajian ini adalah untuk menghasilkan bukti kesahan dan kebolehpercayaan metrik pengukuran kebergunaan perisian kursus matematik secara psikometrik dan seterusnya membentuk rumus skor kebergunaan. Kertas ini membincangkan pembentukan rumus kebergunaan. Berdasarkan pandangan secara teori, satu model hipotesis kebergunaan dengan tiga faktor iaitu kebolehgunaan, kefungsian dan kecekapan dan 85 metrik dicadangkan. Melalui pengesahan pakar dan ujian kebolehpercayaan metrik kebergunaan dikurangkan kepada 64. Instrumen dengan 64 metrik ditadbir ke atas 620 guru matematik di lima zon di Malaysia. Mereka diminta melayari perisian kursus matematik (PKM) sambil melengkapkan instrumen. Analisis faktor penjelajahan dilakukan untuk mengesahkan konstruk kebergunaan manakala analisis faktor pengesahan dijalankan untuk membentuk model berstruktur dengan memadankan 34 metrik dengan model hipotesis. Ujian-t dan ujian ANOVA mendapati tiga pembolehubah iaitu, bidang pengkhususan guru, pengalaman menggunakan komputer dan ralat penilaian menyumbang kepada skor kebergunaan. Dapatan ini digunakan untuk membentuk rumus kebergunaan PKM.
  9. Chakraborty S, Chakravorty R, Alam S, Kabir Y, Mahtab M, Islam MA, et al.
    Euroasian J Hepatogastroenterol, 2020 3 3;9(2):84-90.
    PMID: 32117696 DOI: 10.5005/jp-journals-10018-1303
    Aim: Attainment of sustainable development goal (SDG) targets requires reducing the rate of new hepatitis B virus (HBV)-induced infection and mortality rate to 90% and 65%, respectively, by 2030. Therefore, it is important to investigate the feasibility of reducing the required rates of HBV-induced infection and death incidents at the current rate of vaccination coverage in Bangladesh. Moreover, factors influencing vaccination coverage like negative bias toward girls during immunization can affect the current vaccination program and ultimately hinder the efforts to reduce HBV-induced infection and death rates. To investigate the possibility of reducing HBV-induced infection and death rates with current vaccination coverage, we adopted mathematical molding-based approach.

    Materials and methods: We developed a mathematical model based on the susceptible-infectious-recovered model to simulate the HBV-induced infection in children under the age of five at three different vaccination rates: 80, 90, and 95%. Additionally the impact of current vaccination coverage was assessed on HBV-induced death rates in the future. Moreover, we took advantage of the mathematical model to investigate the impact of negative bias toward girls in vaccination program on HBV-induced infection and death rates.

    Results: The model simulations revealed that 10% increase in the vaccination rate from 80 to 90% can potentially contribute to the significant lowering (around 40%) of HBV-induced infection rate among children. When increased by 5% of vaccination rate from 90 to 95%, the HBV-infection rate is likely to be decreased by another 22%. Likewise, 44% reduction in HBV-induced death rate in the future (2050 onward) can potentially be achieved by 10% increase in the current vaccination rate from 80 to 90%, whereas 5% increase in the current vaccination rate (90-95%) may lead to 24% further reduction of death rate. These results underscored the significant impact of vaccination in reducing HBV-induced infection among children and future death rates in adults. Moreover, at 90% vaccination coverage, the negative bias of vaccination toward girls contributes to an increase of 15 and 12% of HBV-induced infection and death rates, respectively, in female subjects compared to their male counterparts.

    Conclusion: The current vaccination coverage (80-90%) is further aggravated by untimely vaccination, dropouts from vaccination program, and negative bias toward girls in vaccination program. Therefore, if the current situation persists, it will not be possible to accomplish the required reduction in HBV-induced infection and death rates by 2030, according to the SDG guidelines. Moreover negative bias in the vaccination program may intensify the HBV-induced infection and death rates in the future.

    Clinical significance: In light of the mathematical model, we suggest that the vaccination coverage should be increased to 95% without any negative bias toward girls. To accomplish this, the concerning authorities must ensure timely and full completion of the HBV vaccine schedules, reducing dropouts from vaccination program, and lastly preventing negative bias toward girls to uplift vaccination coverage to more than 95% with gender equality. Without these strategies, the necessary reduction in the HBV-induced infection and death rates in Bangladesh may not be attained per SDG directives.

    How to cite this article: Chakraborty S, Chakravorty R, Alam S, et al. A Dynamic Mathematical Modeling Revelation about the Impact of Vaccination on Hepatitis B Virus-induced Infection and Death Rate in Bangladesh. Euroasian J Hepato-Gastroenterol 2019;9(2):84-90.

  10. Azid N.A.,, Md Sani M, Zamry A.A, Ahmad, S, Mohd Ashari N.S., Tan H.T.T., et al.
    MyJurnal
    Total immunoglobulin E (IgE) has been an indicator for early diagnosis of allergic diseases due the important role of IgE in the sensitization towards allergens. Although total IgE is a common diagnostic test that screens for possible allergic diseases, reports are still lacking on the profile of total IgE levels of allergic patients from the north-east region of Peninsular Malaysia. Hence, this study aimed to investigate the association of total IgE levels with clinico-demographic parameters and whether increasing total IgE levels were associated with certain allergic manifestations in this study cohort. A local cohort of allergic patients (n=71) diagnosed in between 2009 and 2016 in Hospital Universiti Sains Malaysia (HUSM) were evaluated for the relevance of total IgE levels in allergy diagnosis. Our study did not demonstrate significant association between total IgE with age (p=0.75) or gender (p=0.65). However, we showed that elevated total IgE levels were associated with disease severity. Our data suggests that increasing total IgE levels beyond the conventional cutoff used to define abnormal IgE levels were more commonly associated with patients presenting allergic asthma and skin allergy manifestations. Total IgE level is a useful indicator for diagnosis of allergic diseases. Although total IgE level might not be sufficient to confirm the sensitization status of an individual, it may reflect the severity of the allergic disease suffered and it has a high correlation with positive sIgE values.
  11. Shah SSM, Luthfi AAI, Low KO, Harun S, Manaf SFA, Illias RM, et al.
    Sci Rep, 2019 03 11;9(1):4080.
    PMID: 30858467 DOI: 10.1038/s41598-019-40807-z
    Kenaf (Hibiscus cannabinus L.), a potential fibre crop with a desirably high growth rate, could serve as a sustainable feedstock in the production of xylitol. In this work, the extraction of soluble products of kenaf through dilute nitric-acid hydrolysis was elucidated with respect to three parameters, namely temperature, residence time, and acid concentration. The study will assist in evaluating the performance in terms of xylose recovery. The result point out that the maximum xylose yield of 30.7 g per 100 g of dry kenaf was attained from 2% (v/v) HNO3 at 130 °C for 60 min. The detoxified hydrolysate was incorporated as the primary carbon source for subsequent fermentation by recombinant Escherichia coli and the performance of strain on five different semi-synthetic media on xylitol production were evaluated herein. Among these media, batch cultivation in a basal salt medium (BSM) afforded the highest xylitol yield of 0.35 g/g based on xylose consumption, which corresponded to 92.8% substrate utilization after 38 h. Subsequently, fermentation by E. coli in the xylose-based kenaf hydrolysate supplemented with BSM resulting in 6.8 g/L xylitol which corresponding to xylitol yield of 0.38 g/g. These findings suggested that the use of kenaf as the fermentation feedstock could be advantageous for the development of sustainable xylitol production.
  12. Sulaiman Mahzan, Siti Fairuz Nurr Sadikan, Mohd Ab Malek Md Shah, Mohd Harun Shahudin, Shamsol Shafie, Mohamad Hafidz Rahmat
    Jurnal Inovasi Malaysia, 2018;2(1):1-16.
    MyJurnal
    Tajweed is one of most important elements in learning of al-Quran recitation accordingly. Long and short or even buzzing (dengung) in quran recitation would give a different meaning. Traditional learning method is still considered as the main option in learning the tajweed but a new requirement that necessarily suits to recent technology and game-based learning method is most preferred among youngsters nowadays. There are a lot of significant relationships between game-based learning with positive impact on learning capabilities. The development of game-based learning or edutainment shows a good potential but perception on tajweed game-based learning or edutainment must be discovered. The methodology of this study is divided into two main components; the development of application of edutainment called as ‘Teroka Tajwid’; and purview study on the perception towardsthe application which has become the main focus of this study. Model of Rapid Application Development (RAD) was chosen as the model of the application development and a quantitative survey was conducted to evaluate the users’ perception on design; audio, video and visual; and navigation and userfriendly. As a result, overall, the survey has shown that the performance of the application is suitable and attractive in learning basic tajweed.
  13. Yusuf A, Mamun ASMA, Kamruzzaman M, Saw A, Abo El-Fetoh NM, Lestrel PE, et al.
    BMC Pediatr, 2019 06 29;19(1):213.
    PMID: 31255172 DOI: 10.1186/s12887-019-1581-9
    BACKGROUND: Anemia is not only a major public health problem among children in developing countries, it is also an important predictor for their future growth and development. The objective of this study was to identify possible factors associated with anemia among pre-school children in Bangladesh after removing a cluster effect of the population, and to determine the prevalence of this condition.

    METHODS: Data for this study was extracted from the 2011 Bangladesh Demographic and Health Survey (BDHS-2011). In this survey, data was collected using a two-stage stratified cluster sampling approach. The chi-square test and a two-level logistic regression model were used for further analysis.

    RESULTS: Data from 2231 children aged 6-59 months were included for analysis. The prevalence of child anemia was noted to be 52.10%. Among these anemic children, 48.40% where from urban environment and 53.90% were from rural areas. The prevalence of mild, moderate and severe anemia among children was 57.10, 41.40 and 1.50% respectively. The two-level logistic regression model revealed that the following factors were associated with childhood anemia: children of anemic mothers (p 

  14. Lai OT, Md. Selim Reza, Abdul Ghani Rafek, Ailie Sofyiana Serasa, Azimah Hussin, Ern LK
    Sains Malaysiana, 2016;45:1603-1607.
    The ultimate bearing capacity is an essential requirement in design quantification for shallow foundations especially
    for structures built on large rock masses. In many engineering projects, structures built on foundation of heavily jointed
    rock masses may face issues such as instability and sudden catastrophic rock slope failure. Determination of the ultimate
    bearing capacity (Qult) of foundations resting on rock mass has traditionally been determined by employing several
    strength criterions. One of the accepted and widely implemented methods is to use the Hoek-Brown failure criterion 2002,
    where the required parameters are determined from a rock mass classification system, Geological Strength Index (GSI).
    This paper defines an assessment for ultimate bearing capacity (Qult) based on the Hoek-Brown failure criterion 2002
    for a granitic rock slope beneath a 20 m diameter concrete water tank at Bandar Mahkota Cheras, Kajang, Selangor.
    Based on the Hoek-Brown failure criterion 2002, the ultimate bearing capacity (Qult) of rock mass was 7.91 MPa. The
    actual stress acting on the rock mass was 0.32 MPa. The assessment showed that the rock mass is safe since the ultimate
    bearing capacity (Qult) is 24.7 times higher than the actual stress acting on the rock mass.
  15. Abdulla MA, Khairul Anuar A, Khalifa S, Salmah I, Suzainur Kulop AR, Md Nazmul HM, et al.
    JUMMEC, 2002;7:118-121.
  16. Mohd Rohaizat Hassan, Mohd Fadhli Samsuri1, Shamsul Azhar Shah, Nazarudin Safian, Zulkifli Md Zainuddin, Hasanain Faisal Ghazi
    The purpose of this study was to determine the prevalence and factors associated with of Premature Ejaculation &Erectile Dysfunctionamong Malaysian men.A cross-sectional study was conducted in urban and rural areas using standardized and validated self-administeredquestionnaires.A total of 319 samples were recruited for PE screening. The overall prevalence of PE was 31.7% with 20.7% and 40.8% for urban and rural arearespectively. Depression, anxiety and frequent masturbation were significantly associated with PE. While For ED, infrequent sexual intercourse was the sole factor significantly associated with ED.PE and ED were highly prevalent in both urban and rural areas; with several significant preventable associated factors.
  17. Nurul Balqis Md Dzali, Mohd Nizam Zahary, Nor Hidayah Abu Bakar, Hasnan Jaafar, Wan Rohani Wan Taib
    Brain tumour occurrence in Malaysia demonstrates an increasing trend from year to year among adults and the second most common cancer among children. Thus, the expansion of numerous research for novel therapy and treatment are necessary. The distribution of brain tumour in a specific population is important to provide substantial information about the current trends for developing new diagnostic technique and research. Consequently, this study is opted to provide descriptive data of brain tumour in Hospital Universiti Sains Malaysia (USM). 217 brain tumour cases were collected from the hospital record between 2011 and 2014. The brain tumour cases were confirmed by pathologists according to WHO classification and grading. Descriptive analysis was evaluated by using Microsoft Excel and IBM SPSS version 22. Gender preponderance in this study shows very little difference. The most common adult primary brain tumour in this study was meningioma (32.7%) followed by glioblastoma (7.8%), a type of diffuse astrocytic tumour. According to age factor, brain tumour distribution pattern shows an increasing trend as the age increases and meningioma is the most common among the elder patients. Secondary tumour takes more than 10% from overall percentage of brain tumour cases. In conclusion, the descriptive data presentation in this study is very helpful to provide baseline information on the current brain tumour occurrence in this region.
  18. Nurulhuda Md Amin, Nurul Hafizah Mohd Norizan, Nor Fadzillah Abdul Jalil, Raja Norliza Raja Omar, Mushawiahti Mustapha
    MyJurnal
    Posterior capsular rupture (PCR) is a common and serious complication of cataract surgery. It complicates the process of inserting posterior chamber intraocular lens and affects the visual outcome. This study is a review on visual acuity of patients that had PCR during cataract surgery in Hospital Melaka, risk factors of PCR, and factors that cause poor visual outcome. The record of all patients that had PCR during cataract surgery from 1 January 2014 till 31 March 2017 was traced using National Eye Database (NED) and the patients’ folders were reviewed retrospectively. Data collected was demographic data, type of cataract surgery, status of surgeon, underlying ocular diseases, risks factors for PCR, postoperative visual acuity, and factors that cause poor visual outcome. There were 238 eyes that had PCR during cataract surgery, from 126 (53%) female and 112 (47%) male patients. The mean age was 66.62 years old, ranging from 9 to 87 years old. Major risk factors identified were hard cataract, polar cataract, uncooperative patients, extended continuous capsulorhexis, subluxated lens, myopia, vitrectomised eyes, and poor pupil dilatation. From 153 cases that had no pre-existing ocular diseases, 119 (78%) cases had normal vision (best corrected visual acuity – BCVA) two months after surgery, 10 (7%) cases had moderate visual impairment, and four (2%) cases had severe visual impairment. Causes of BCVA poorer than 6/18 were high astigmatism, CMO, and prolonged inflammation. Cataract surgery, even complicated by PCR, is compatible with good visual outcome if the complications are managed promptly.
  19. Nur Izzati Md Saleh, (Wan Azlina Wan Ab Karim Ghani, Mohd Razif Harun, Siti Mazlina Mustapa Kamal
    MyJurnal
    In the present research, microalgae protein hydrolysate of Nannochloropsis gaditana (MPH) was extracted via enzymatic hydrolysis using alcalase enzyme. Hydrolysis conditions like (pH, temperature, enzyme concentration and substrate concentration) were optimized by Response Surface Methodology (RSM) using Central Composite Design (CCD). Four range of independent variables namely; pH (7-9), temperature (45-55°C), substrate concentration (2-6 g/L) and enzyme concentration (0.2-0.4 g/L) were used to study the influence of these parameters on the degree of hydrolysis. The CCD consisted of twenty-four experimental points and six replicates of central points with the optimum conditions obtained from this experiment were at pH 8.14, a temperature 51.4°C, a substrate concentration 5.48 g/L and an enzyme concentration 0.26 g/L with maximum degree hydrolysis of 55.76%. All experiments were fixed at 24 hours reaction time. The degree of hydrolysis of MPH was analysed using O-phthaldehyde (OPA) method to quantify the cleavage of peptide bond. The optimized sample was evaluated for its antioxidant activity using 1,1-Diphenyl-2-picrylhydrazyl (DPPH) assay with 52.19% and 2, 2'- azino-bis (ethylbenzthiazoline-6-sulfonic acid (ABTS) assay with 14.13%. The bioactive peptides contained in Nannochloropsis gaditana have the ability to scavenge free radicals and act as good antioxidants.
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