Displaying publications 3061 - 3080 of 8501 in total

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  1. Lee WY, Tan JTA, Kok JK
    Psychol Stud (Mysore), 2022;67(3):385-400.
    PMID: 35529704 DOI: 10.1007/s12646-022-00658-7
    The sense of belonging plays an important role in a person's well-being. It also acts as a protective factor against mental distress. Deaf people struggle to fit into the dominant hearing society due to communication barriers. The multi-languages used in Malaysian families add to this communication challenge. Communication breakdown leaves the deaf person socially excluded while a poor sense of belonging also increases their vulnerability to mental health issues. Hence, this study explored the deaf person's sense of belonging and well-being through their social experiences in Malaysia. Baumeister's need-to-belong theory served as the theoretical framework of this study. An Ethnographic approach with in-depth interviews and participant observation was used to explore the social experiences of six Chinese deaf adults, aged 20-37, residing in Ipoh. Their hearing parents were interviewed as well to obtain parents' perspective of the child's well-being in the earlier years. Participants were selected using the purposive and snowballing sampling method. Upon data saturation, thematic analysis was used to identify themes, patterns, and interpret its meanings. The main themes that emerged from the data was the "struggle to fit in", "emotional effect", and "quality social connections". Findings of this study can be used in the formation of policies and strategies to ensure that deaf individuals are not excluded from their communities. Cultivating an inclusive community and developing the deaf community offers a better sense of belonging, which would help increase well-being of deaf people. Future research may focus on deaf identity and well-being of deaf youth in Malaysia.
  2. Tan HL, Yakkaphan P, Beke A, Renton T
    J Oral Facial Pain Headache, 2024 Mar;38(1):93-105.
    PMID: 39788579 DOI: 10.22514/jofph.2024.009
    This case series aimed to assess the treatment outcomes of onabotulinum toxin A (BTX-A) in patients with refractory posttraumatic trigeminal neuropathic pain (PTNP) and to conduct a narrative review of the evidence for BTX-A in PTNP. Thirteen patients were treated with BTX-A infiltrations. Patient demographic and pain characteristics, BTX-A administration, and treatment outcomes were retrospectively analyzed. Papers retrieved after a literature search of articles on PTNP treatment using BTX-A were reviewed. Six patients reported an improvement in pain 3 months after the initial BTX-A injection, with 4 patients reporting a 50% reduction. Two patients achieved an 80% reduction in pain score over 3 years of BTX-A therapy. Three patients reported temporary ipsilateral facial muscle weakness. The literature review revealed five case reports on the use of BTX-A in PTNP patients that reported similar effectiveness to the present cohort study. BTX-A may be a potential treatment modality for refractory PTNP, thus reducing the need for polypharmacy. Multiple intraoral BTX-A injections administered over the painful sites are well tolerated, safe and easily practiced. High-quality studies are required to evaluate the long-term therapeutic efficacy and side effects of BTX-A therapy.
  3. Tan J, Feng L, Ragavan ND, Chai Theam O, Li X
    Biochem Biophys Res Commun, 2024 Dec 31;741:151013.
    PMID: 39591906 DOI: 10.1016/j.bbrc.2024.151013
    This study investigates the role of Caspase-11 in Chronic Kidney Disease (CKD) and examines the therapeutic potential of inhibiting Caspase-11 using exosome-mediated siRNA. We established a CKD rat model and analyzed the expression of Caspase-11 through immunohistochemistry. The study involved overexpressing Caspase-11 using an adeno-associated virus (AAV) and constructing exosomes loaded with siRNA targeting Caspase-11 (exo-si-Caspase-11). Renal tissue damage and fibrosis were assessed using H&E staining, Masson's trichrome, TUNEL assay, and Sirius Red staining. Additionally, urinary protein and blood urea nitrogen (BUN) levels were measured, alongside analyses of serum calcium and phosphorus levels. H&E staining was performed to evaluate the effects of exo-si-Caspase-11 on damage to the heart, liver, spleen, and lungs. The results showed that the CKD model group experienced significant weight loss, increased blood pressure, and elevated Caspase-11 expression. AAV-mediated Caspase-11 overexpression led to substantial renal fibrosis, increased apoptosis, and elevated urinary protein and BUN levels. Additionally, the group with Caspase-11 overexpression exhibited elevated serum calcium and phosphorus levels. Conversely, treatment with exo-si-Caspase-11 reduced these pathological changes in renal tissue without causing damage to other major organs. These findings suggest that exosome-mediated siRNA delivery targeting Caspase-11 is an effective therapeutic strategy for CKD.
  4. Wong BC, Ling FY, Ayub Q, Tan HS
    Biochem Biophys Res Commun, 2025 Mar 25;754:151546.
    PMID: 40023989 DOI: 10.1016/j.bbrc.2025.151546
    Identifying essential genes in bacterial pathogens during infection can enhance knowledge and provide novel targets for antimicrobial agents' development. Currently, only Shigella flexneri essential genes during in vitro growth have been experimentally identified. This study used transposon insertion sequencing (TIS) to identify Shigella sonnei essential genes during Caenorhabditis elegans infection. 498 genes were predicted to be essential in S. sonnei during growth on nutrient-rich media. Some genes previously predicted to be essential in Shigella were found non-essential in S. sonnei, such as acetyl metabolism genes (aceEF, lpdA) and sulphate transport genes (cysA, cyst, cysW). Finally, 217 genes were predicted as S. sonnei virulence genes during infection, including acid resistance and biofilm formation genes which was not linked to S. sonnei virulence previously.
  5. Wong WY, Ismail SM, Phan CW, Tan YS
    Int J Med Mushrooms, 2024;26(9):17-31.
    PMID: 39093399 DOI: 10.1615/IntJMedMushrooms.2024054164
    Cellular damage resulting from elevated levels of free radicals can lead to persistent health issues. Pleurotus floridanus, an edible white oyster mushroom, is rich in β-glucans with potent antioxidant and anti-inflammatory properties. In this research, we examined the β-glucan content, total phenolic content, as well as antioxidant and anti-inflammatory potential of hot water extracts with varying particle sizes (< 75, 75-154, 154-300, and 300-600 μm) of both whole and sliced fruiting bodies of P. floridanus. The findings revealed that the в-glucan content increased as the particle size increased, although no significant differences were observed. Conversely, smaller particle sizes (< 75 μm) of whole and sliced fruiting bodies of P. floridanus exhibited higher phenolic content, 2,2-diphenyl-1-picryl-hy-drazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging activity, and reducing ability compared with larger particle size (> 75 μm). Of the four samples (AW2, AW3, AS1, and AS2) with the highest antioxidant activity selected for anti-inflammatory assays, all demonstrated the ability to reduce nitric oxide and tumor necrosis factor-alpha levels, but did not enhance interleukin-10 expression in lipopolysaccharide-stimulated RAW264.7 cells. Interestingly, particle size < 75 to 300 μm did not appear to influence the anti-inflammatory activity, because no significant differences were observed among the particle sizes. Therefore, a particle size < 300 μm in a P. floridanus hot water extract could serve as a valuable source of antioxidant and anti-inflammatory compounds to counteract the harmful effects of free radicals.
  6. Sathiaseelan A, Song KP, Tan HS, Choo WS
    FEMS Microbiol Lett, 2025 Jan 10;372.
    PMID: 40128011 DOI: 10.1093/femsle/fnaf035
    This study investigated the antibiofilm effects of Clitoria ternatea flowers anthocyanin fraction (AF) on Streptococcus mutans, Actinomyces viscosus, and Aggregatibacter actinomycetemcomitans. AF was obtained using column chromatography, and liquid chromatography-mass spectrometry was employed for its characterization and identification. The crystal violet assay and scanning electron microscopy analysis revealed significant inhibition of early biofilm formation and destruction of preformed biofilms after AF treatment (0.94-15 mg ml-1). Antiadhesion assay on acrylic teeth demonstrated that AF effectively hampered sucrose dependent and independent attachment. Importantly, growth curve and pH drop assays showed that AF inhibited pH reduction for all bacteria tested without hindering bacterial growth. Furthermore, the tetrazolium-based cytotoxicity assay indicated no toxicity towards normal human gingival fibroblasts at 0.78-12.5 mg ml-1. These findings suggest C. ternatea anthocyanins are promising antibiofilm agents for oral biofilm control, acting during both initial formation and on mature biofilms.
  7. Boon WJ, Tan GC, Hamizan AKW
    Indian J Otolaryngol Head Neck Surg, 2025 Mar;77(3):1603-1606.
    PMID: 40093428 DOI: 10.1007/s12070-024-05303-5
    Angioleiomyoma is a rare benign smooth muscle tumor that is uncommonly encountered within the nasal cavity and presents with non-specific symptoms. Biopsy with histopathology examination is required to establish the diagnosis. The primary therapy for nasal angioleiomyoma is total surgical excision and follow up is recommended to identify local recurrence. Here, we present a case of angioleiomyoma found in the inferior turbinate and provide an overview of the relevant literature.
  8. Tan EW, Low LH, Atanasov AG, Goh BH
    Pharmacol Res Perspect, 2025 Feb;13(1):e70074.
    PMID: 39929745 DOI: 10.1002/prp2.70074
    of the key disciplines that equip next-generation researchers engaged in ethnopharmacology research with the necessary knowledge and skills to navigate the transition from traditional ethnopharmacology to modern drug discovery.
  9. Cheah YK, Lim KK, Kee CC, Tan LK
    PMID: 39579044 DOI: 10.1177/17579139241296476
    AIMS: Exposure to passive smoking among adolescents is a public health concern. This study aims to explore demographic and lifestyle factors associated with perception of passive smoking risk and the mediating effects of exposure to anti-smoking advertisements and education about the dangers of smoking on the relationship between grade levels and the perception.

    METHOD: Data from the Tobacco and E-Cigarette Survey among Malaysian Adolescents (n = 13,117) were analysed. Ordered probit models were used to assess factors associated with passive smoking risk perception (i.e. not harmful, probably not harmful, probably harmful, harmful).

    RESULTS: About one-third of adolescents did not perceive that passive smoking was harmful (30.4%). Adolescents with the perception that passive smoking was not harmful were more likely to be students at low grade levels, be males, be non-Malays and be low-pocket-money recipients and those who used e-cigarettes and who smoked. Being aware of anti-smoking advertisements (direct effect = 0.174; indirect effect = 0.012) and taught on the dangers of smoking (direct effect = 0.179; indirect effect = 0.003) partially mediated the positive relationship between grade levels and perceived risk of passive smoking.

    CONCLUSION: Policies implemented to reduce exposure to passive smoking should highlight the important role of demographic factors, lifestyle profiles, anti-smoking advertisements and education about the dangers of smoking in improving passive smoking risk perception among adolescents.

  10. Liu Z, Lee YY, Tan CP, Wang Y, Qiu C
    Food Chem, 2025 Feb 01;464(Pt 1):141553.
    PMID: 39406140 DOI: 10.1016/j.foodchem.2024.141553
    Cyclolinopeptides (CLs) have anti-inflammatory, anti-osteoporosis, and anti-tumor effects, however, low water and oil solubility greatly limit their application. Herein, CLs-loaded β-cyclodextrin (β-CD) emulsions were prepared with different oil phases. The in vitro digestibility, cellular absorption, and anti-inflammatory effects were evaluated. Camellia oil diacylglycerol (CO DAG) showed enhanced dissolving ability for CLs due to high polarity. β-CD formed inclusion complexes with DAG through hydrogen bond and the emulsions showed smaller size and higher physical stability with 50 % (w/w) oil. The in vitro digestibility of the DAG emulsion was increased and the CLs' bioavailability was 13.6-fold higher than CLs in oil. The CLs-loaded Pickering emulsion digesta exhibited a higher nitric oxides (NO) inhibition rate (58.62 %) and Caco-2 cell penetration (3.09 × 10-6 cm/s). Therefore, emulsion formulated with β-cyclodextrin and DAG can effectively improve the solubility and bioavailability of CLs, which has significant potential for application in functional foods and pharmaceutical industry.
  11. Lee JX, Tan YJ, Ismail NAS
    Int J Mol Sci, 2024 Nov 15;25(22).
    PMID: 39596340 DOI: 10.3390/ijms252212275
    NPHS1 and NPHS2 are kidney gene components that encode for nephrin and podocin, respectively. They play a role in the progression of congenital (CNS) and steroid-resistant (SRNS) nephrotic syndrome. Hence, this study aimed to determine the prevalence and renal outcomes of NPHS mutations among pediatric patients with CNS and SRNS. We also aimed to identify potential predictors of NPHS mutations in this patient cohort. Overall, this study included 33 studies involving 2123 patients screened for NPHS1, whereas 2889 patients from 40 studies were screened for NPHS2 mutations. The patients' mean age was 4.9 ± 1 years (ranging from birth to 18 years), and 56% of patients were male (n = 1281). Using the random-effects model, the pooled proportion of NPHS1 mutations among pediatric patients with CNS and SRNS was 0.15 (95% CI 0.09; 0.24, p < 0.001, I2 = 92.0%). The pooled proportion of NPHS2 mutations was slightly lower, at 0.11 (95% CI 0.08; 0.14, p < 0.001, I2 = 73.8%). Among the 18 studies that reported ESRF, the pooled proportion was 0.47 (95% CI 0.34; 0.61, p < 0.001, I2 = 75.4%). Our study showed that the NPHS1 (β = 1.16, p = 0.35) and NPHS2 (β = 5.49, p = 0.08) mutations did not predict ESRF in CNS and SRNS pediatric patients. Nevertheless, patients from the European continent who had the NPHS2 mutation had a significantly higher risk of developing ESRF (p < 0.05, β = 1.3, OR = 7.97, 95% CI 0.30; 2.30) compared to those who had the NPHS1 mutation. We recommend NPHS mutation screening for earlier diagnosis and to avoid unnecessary steroid treatments. More data are needed to better understand the impact of NPHS mutations among pediatric patients with CNS and SRNS.
  12. Yong TL, Zaman R, Rehman N, Tan CK
    Exp Dermatol, 2025 Feb;34(2):e70042.
    PMID: 39912256 DOI: 10.1111/exd.70042
    Ceramide has transitioned from an incidental discovery to a vital element in skincare, becoming a thoroughly studied compound in the quest to treat skin conditions. Creating a moisture barrier, preserving hydration, regulating pH, controlling inflammation, and enhancing skin functions and appearance are among its established benefits. It is often used medically to repair skin barrier defects, as observed in inflammatory skin conditions like atopic dermatitis (AD) and dry skin types. Furthermore, ceramide and its metabolites are commonly used as predictors before disease manifestation and for prognostication processes, thus can be used as biomarker for clinical diagnosis as well. In the last couple of decades, momentum was also seen in the pre-clinical studies involving anti-cancer and nanotechnology field, whereby ceramide was also used as a drug, a carrier, or even adjunct formulation to increase efficacy of treatment such as chemotherapy. Approaches to increase ceramide levels include directly replenishing lost ceramides with natural extracts, synthetic pseudo-ceramides, or ceramide-like analogues, as well as using supplements that stimulate the body's natural ceramide production. Although ceramide is a well-known treatment in skincare and for common skin conditions like AD and psoriasis, its development and related pharmacology for severe skin conditions, such as skin cancer, remain in pre-clinical stages. Hence, the purpose of this research is to explore the role of ceramide in skin health and its application in common skin diseases.
  13. Tan FY, Mohd Nor NAB, Ahmad SF, Hariri F
    PMID: 39910968 DOI: 10.1177/10556656251319642
    BACKGROUND: Conventionally, vector guidance for internal distractor placement during cleft maxillary distraction osteogenesis (DO) is done using vector guidance splint. Vector guidance splint cannot be fabricated if occlusion is not decompensated for model surgery.

    SOLUTION: Using mandibular occlusal plane as anatomical reference, vector guidance oral prosthesis is fabricated to guide distractor placement and vector determination, in cases where model surgery is not possible.

    WHAT WE DO THAT IS NEW: Vector guidance oral prosthesis is a new prototype produced to guide maxillary DO without the need of model surgery.

  14. Tan LL, Mohamad NS, Hassan NI, Goh CT
    PLoS One, 2025;20(2):e0315974.
    PMID: 39919092 DOI: 10.1371/journal.pone.0315974
    Zinc has been demonstrated to boost immune response during SAR-CoV-2 infection, where it prevents coronavirus multiplication. Clinical investigations have testified to its beneficial effects on respiratory health and its deficiency may reduce immune function. A highly sensitive detection of Zn(II) ion via differential pulse voltammetry (DPV) utilizing an environmentally friendly modified screen-printed carbon electrode (SPCE) of electrochemically reduced graphene oxide (ErGO) embedded with carboxylated-8-carboxamidoquinoline (CACQ) as Zn(II) chelating ligand. The green CACQ/ErGO-modified SPCE was characterized by spectroscopy techniques, such as Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, and field-emission scanning electron microscopy with energy dispersive X-ray (FESEM-EDX). The modified electrode-solution interface was studied by electrochemical cyclic voltammetry (CV) and DPV methods. The CACQ-modified wrinkled ErGO electrode conferred a large surface-to-volume ratio with multiple binding sites resulting in greater opportunity for multiple dative covalent binding events with Zn(II) via coordination chemistry, and considerably accelerated the electron transfer rate at the electrode surface. The green Zn(II) sensor demonstrated a quick response time (60 s), broad linear range [1 pM-1 μM Zn(II) ion], a limit of detection (LOD) of 0.53 pM, 35 days of storage period (≥80% of its initial response retained), good reproducibility [relative standard deviation (RSD) = 3.4%], and repeatability (RSD = 4.4%). The developed electrode was applied to determine Zn(II) ion concentration in dietary supplement samples, and the results were in good agreement with those obtained from inductively coupled plasma-mass spectrometry (ICP-MS).
  15. Tan WF, Robinson S, Tang MM, Malaysian Psoriasis Registry Working Group
    Clin Dermatol, 2024;42(6):616-624.
    PMID: 39278515 DOI: 10.1016/j.clindermatol.2024.09.017
    Nail psoriasis affects 20% to 30% of psoriasis patients and is an early predictor of psoriatic arthritis (PsA). We evaluated the prevalence, clinical characteristics, and impact on quality of life of patients with nail psoriasis. We conducted a multicenter retrospective cohort study of patients registered with The Malaysian Psoriasis Registry from January 1, 2007 to December 31, 2020. Of the 24,147 patients, 13,081 (54.2%) had nail psoriasis. Patients with nail psoriasis had later onset of psoriasis (34.0 ± 16.6 years vs 32.9 ± 17.6 years, P < .001) and longer disease duration (11.4 ± 10.5 years vs 8.5 ± 9.4 years, P < .01), with a man-to-woman ratio of 1.2:1. They were more likely to have a family history of psoriasis, cardiometabolic diseases, smoking history, higher body mass index, severe disease, PsA, face and scalp involvement, and higher mean Dermatology Life Quality Index scores (9.36 ± 6.84 vs 8.87 ± 6.60). Systemic treatment and biologics were more commonly prescribed in this cohort (25.0% vs 13.2%, P < .001). Overall, 54.2% of the Malaysian Psoriasis Registry patients had nail involvement. Nail psoriasis was associated with longer duration of psoriasis, older age of onset, male sex, and a family history of psoriasis. It proved to be an important predictor for PsA, severe psoriasis, face and scalp involvement, increased cardiometabolic risk, and a greater impairment of quality of life.
  16. Wong KT, Hooi YT, Tan SH, Ong KC
    Pathology, 2025 Mar;57(2):230-240.
    PMID: 39799084 DOI: 10.1016/j.pathol.2024.11.003
    Viral infections of the central nervous system (CNS) have been emerging and re-emerging worldwide, and the Australasia region has not been spared. Enterovirus A71 and enterovirus D68, both human enteroviruses, are likely to replace the soon-to-be eradicated poliovirus to cause global outbreaks associated with neurological disease. Although prevalent elsewhere, the newly emergent orthoflavivirus, Japanese encephalitis virus (genotype IV), caused human infections in Australia in 2021, and almost certainly will continue to do so because of spillovers from the natural animal host-vector life cycle endemic in the country. Another orthoflavivirus, Murray Valley encephalitis virus, has re-emerged in Australia. The Hendra henipavirus together with Nipah henipavirus ​are listed as high-risk pathogens by the World Health Organization because both can cause lethal encephalitis. The former remains a health threat in Australasia because bats may still be able to spread the infection to unvaccinated Australian horses and other animals acting as intermediate hosts, and thence to humans. The global COVID-19 pandemic, caused by the emerging severe acute respiratory syndrome coronavirus-2, a virus transmitted from animals to humans that was first described and first arose in China, is associated with acute and long-lasting CNS pathology. Fortunately, the pathology and pathogenesis of these important neurotropic viruses are now better understood, leading to better management protocols and prevention strategies. Pathologists are in a unique position to contribute to the diagnosis and advancement in our knowledge of infectious diseases. This review summarises some of the current knowledge about a few important emerging and re-emerging CNS infections in Australasia and beyond.
  17. Chang WJ, Liew SY, Kurz T, Tan SP
    Beilstein J Org Chem, 2025;21:596-600.
    PMID: 40099301 DOI: 10.3762/bjoc.21.46
    The hydantoin scaffold is renowned for its wide-ranging biological activities, including antibacterial, antiviral, anticancer, anti-inflammatory, and anticonvulsant effects. In this study, we present an innovative, sustainable approach to synthesizing hydantoins (H2a-j) directly from amino acids. This method employs a column chromatography-free, two-step, one-pot microwave-assisted synthesis that delivers hydantoins in yields ranging from 34% to 89%. The protocol demonstrates exceptional functional group tolerance, accommodating phenyl, aliphatic, phenol, alcohol, heterocyclic, and sulfide groups. This scalable, rapid, and eco-friendly strategy offers a promising avenue for the efficient synthesis of hydantoins, aligning with green chemistry principles and expanding the accessibility of these bioactive compounds for pharmaceutical applications.
  18. Tan MK, Muhammad AA, Gorochov AV, Abdullah NA
    Zootaxa, 2024 Aug 29;5501(1):1-38.
    PMID: 39647127 DOI: 10.11646/zootaxa.5501.1.1
    Orthopteran surveys were recently conducted at the Panti Forest Reserve in Johor State of Peninsular Malaysia. Based on new material, three species new to science are described here: Agryllus sori Tan, Muhammad & Gorochov, sp. nov. (Gryllidae: Gryllinae), Malayzacla panti Tan, Muhammad & Gorochov, gen. et sp. nov. (Phalangopsidae: Phalangopsinae) and Parendacustes (Parendacustes) aduncus Tan, Muhammad & Gorochov, sp. nov. (Phalangopsidae: Phalangopsinae). The calling songs of five species are described here for the first time: Agryllus sori Tan, Muhammad & Gorochov, sp. nov., Duolandrevus (Bejorama) firmus tiomani Gorochov, 2016 (Gryllidae: Landrevinae), Malayzacla panti Tan, Muhammad & Gorochov, gen. et sp. nov., Parendacustes (Parendacustes) aduncus Tan, Muhammad & Gorochov, sp. nov. and Trellius (Protrellius) helverseni tioman Gorochov, 2011 (Phalangopsidae: Phaloriinae). New locality records in Panti Forest Reserve for five species are also presented here: Duolandrevus (Bejorama) firmus tiomani Gorochov, 2016, Duolandrevus (Bejorama) parvulus Gorochov, 2016, Brevimunda trilineata Tan, Japir, Chung & Robillard, 2022 (Oecanthidae: Podoscirtinae), Zamunda fuscirostris (Chopard, 1969) (Oecanthidae: Podoscirtinae) and Trellius (Protrellius) helverseni tioman Gorochov, 2011.
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