Displaying publications 21 - 40 of 67 in total

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  1. Fulltext Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611
    Dinarvand M, Rezaee M, Masomian M, Jazayeri SD, Zareian M, Abbasi S, et al.
    Biomed Res Int, 2013;2013:508968.
    PMID: 24151605 DOI: 10.1155/2013/508968
    The study is to identify the extraction of intracellular inulinase (exo- and endoinulinase) and invertase as well as optimization medium composition for maximum productions of intra- and extracellular enzymes from Aspergillus niger ATCC 20611. From two different methods for extraction of intracellular enzymes, ultrasonic method was found more effective. Response surface methodology (RSM) with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. The effect of five main reaction parameters including sucrose, yeast extract, NaNO₃, Zn⁺², and Triton X-100 on the production of enzymes was analyzed. A modified quadratic model was fitted to the data with a coefficient of determination (R²) more than 0.90 for all responses. The intra-extracellular inulinase and invertase productions increased in the range from 16 to 8.4 times in the optimized medium (10% (w/v) sucrose, 2.5% (w/v) yeast extract, 2% (w/v) NaNO₃, 1.5 mM (v/v) Zn⁺², and 1% (v/v) Triton X-100) by RSM and from around 1.2 to 1.3 times greater than in the medium optimized by one-factor-at-a-time, respectively. The results of bioprocesses optimization can be useful in the scale-up fermentation and food industry.
    Matched MeSH terms: Aspergillus niger/enzymology*
  2. Fulltext Optimizing culture conditions for production of intra and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 by response surface methodology (RSM)
    Dinarvand M, Rezaee M, Foroughi M
    Braz J Microbiol, 2017 Jul-Sep;48(3):427-441.
    PMID: 28359854 DOI: 10.1016/j.bjm.2016.10.026
    The aim of this study was obtain a model that maximizes growth and production of inulinase and invertase by Aspergillus niger ATCC 20611, employing response surface methodology (RSM). The RSM with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R2) more than 0.90 for all responses. This model adequately explained the data variation and represented the actual relationships between the parameters and responses. The pH and temperature value of the cultivation medium were the most significant variables and the effects of inoculum size and agitation speed were slightly lower. The intra-extracellular inulinase, invertase production and biomass content increased 10-32 fold in the optimized medium condition (pH 6.5, temperature 30°C, 6% (v/v), inoculum size and 150rpm agitation speed) by RSM compared with medium optimized through the one-factor-at-a-time method. The process development and intensification for simultaneous production of intra-extracellular inulinase (exo and endo inulinase) and invertase from A. niger could be used for industrial applications.
    Matched MeSH terms: Aspergillus niger/enzymology; Aspergillus niger/genetics; Aspergillus niger/growth & development; Aspergillus niger/metabolism*
  3. Fulltext Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system
    El Enshasy HA, Elsayed EA, Suhaimi N, Malek RA, Esawy M
    BMC Biotechnol, 2018 11 09;18(1):71.
    PMID: 30413198 DOI: 10.1186/s12896-018-0481-7
    BACKGROUND: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4 billion by 2020.

    RESULTS: The production medium was first optimized using a statistical optimization approach to increase pectinase production. A maximal enzyme concentration of 76.35 U/mL (a 2.8-fold increase compared with the initial medium) was produced in a medium composed of (g/L): pectin, 32.22; (NH4)2SO4, 4.33; K2HPO4, 1.36; MgSO4.5H2O, 0.05; KCl, 0.05; and FeSO4.5H2O, 0.10. The cultivations were then carried out in a 16-L stirred tank bioreactor in both batch and fed-batch modes to improve enzyme production, which is an important step for bioprocess industrialization. Controlling the pH at 5.5 during cultivation yielded a pectinase production of 109.63 U/mL, which was about 10% higher than the uncontrolled pH culture. Furthermore, fed-batch cultivation using sucrose as a feeding substrate with a rate of 2 g/L/h increased the enzyme production up to 450 U/mL after 126 h.

    CONCLUSIONS: Statistical medium optimization improved volumetric pectinase productivity by about 2.8 folds. Scaling-up the production process in 16-L semi-industrial stirred tank bioreactor under controlled pH further enhanced pectinase production by about 4-folds. Finally, bioreactor fed-batch cultivation using constant carbon source feeding increased maximal volumetric enzyme production by about 16.5-folds from the initial starting conditions.

    Matched MeSH terms: Aspergillus niger/genetics; Aspergillus niger/growth & development; Aspergillus niger/metabolism*
  4. Domestic wastewater biosolids accumulation by liquid state bioconversion process for rapid composting
    Fakhru'l-Razi A, Alam MZ, Idris A, Abd-Aziz S, Molla AH
    J Environ Sci Health A Tox Hazard Subst Environ Eng, 2002 Sep;37(8):1533-43.
    PMID: 12369644
    Bioconversion of higher strength of domestic wastewater biosolids (sludge) (4% w/w of TSS) by mixed fungal culture of Aspergillus niger and Penicillium corylophilum was studied in a laboratory. The effect of potential mixed fungi on domestic wastewater sludge accelerated the liquid state bioconversion (LSB) process. The highest production of dry sludge cake (biosolids) was enriched with fungal biomass to about 85.66 g/kg containing 25.23 g/kg of protein after 8 days of treatment. The results presented in this study revealed that the reduction of chemical oxygen demand (COD), total suspended solid (TSS), and specific resistance to filtration (SRF) of treated sludge were highly influenced by the fungal culture as compared to control (uninnoculated). The maximum removal rates in treated sludge (biosolids) supernatant recorded were 92% of COD and 98.8% of TSS. Lower SRF (1.08 x 10(12) m/kg) was perceived in microbially treated sludge after 6 days of fermentation. The observed parameters were highly influenced after 8 days of treatment. The influence of pH was also studied and presented in the paper.
    Matched MeSH terms: Aspergillus niger/growth & development*; Aspergillus niger/isolation & purification
  5. Fulltext Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit
    Hendra R, Ahmad S, Sukari A, Shukor MY, Oskoueian E
    Int J Mol Sci, 2011;12(6):3422-31.
    PMID: 21747685 DOI: 10.3390/ijms12063422
    Phaleria macrocarpa (Scheff.) Boerl (Thymelaceae) is commonly known as 'Crown of God', 'Mahkota Dewa', and 'Pau'. It originates from Papua Island, Indonesia and it grows in tropical areas. Empirically, it is potent in treating the hypertensive, diabetic, cancer and diuretic patients. It has a long history of ethnopharmacological usage, and the lack of information about its biological activities led us to investigate the possible biological activities by characterisation of flavonoids and antimicrobial activity of various part of P. macrocarpa against pathogenic bacteria and fungi. The results showed that kaempferol, myricetin, naringin, and rutin were the major flavonoids present in the pericarp while naringin and quercetin were found in the mesocarp and seed. Furthermore, the antibacterial activity of different parts of P. macrocarpa fruit showed a weak ability to moderate antibacterial activity against pathogenic tested bacteria (inhibition range: 0.93-2.17 cm) at concentration of 0.3 mg/disc. The anti fungi activity was only found in seed extract against Aspergillus niger (1.87 cm) at concentration of 0.3 mg/well. From the results obtained, P. macrocarpa fruit could be considered as a natural antimicrobial source due to the presence of flavonoid compounds.
    Matched MeSH terms: Aspergillus niger/drug effects
  6. Fulltext Hydroxypropylcellulose as a novel green reservoir for the synthesis, stabilization, and storage of silver nanoparticles
    Hussain MA, Shah A, Jantan I, Shah MR, Tahir MN, Ahmad R, et al.
    Int J Nanomedicine, 2015;10:2079-88.
    PMID: 25844038 DOI: 10.2147/IJN.S75874
    Polysaccharides are attracting the vigil eye of researchers in order to design the green synthesis of silver nanoparticles (Ag NPs) of diverse size, shape, and application. We report an environmentally friendly method to synthesize Ag NPs where no physical reaction conditions were employed. Hydroxypropylcellulose (HPC) was used as a template nanoreactor, stabilizer, and capping agent to obtain Ag NPs. Different concentrations of AgNO3 solutions (50 mmol, 75 mmol, and 100 mmol) were mixed with a concentrated aqueous solution of HPC and the progress of the reaction was monitored by noting color changes of the reaction mixture at different reaction times for up to 24 hours. Characteristic ultraviolet-visible spectroscopy (UV/Vis) absorption bands of Ag NPs were observed in the range of 388-452 nm. The morphology of the Ag NPs was studied by scanning electron microscopy, transmission electron microscopy (TEM), and atomic force microscopy. The TEM images confirmed that the size of the Ag NPs was in the range of 25-55 nm. Powder X-ray diffraction studies showed that the crystal phase of the Ag NPs was face-centered cubic. The as-prepared Ag NPs were found to be stable, and no changes in size and morphology were observed after storage in HPC thin films over 1 year, as indicated by UV/Vis spectra. So, the present work furnishes a green and economical strategy for the synthesis and storage of stable Ag NPs. As-synthesized Ag NPs showed significant antimicrobial activity against different bacterial (Escherichia coli, Staphylococcus epidermidis, S. aureus, Bacillus subtilis, Pseudomonas aeruginosa) and fungal strains (Actinomycetes and Aspergillus niger).
    Matched MeSH terms: Aspergillus niger/drug effects
  7. Fungal solid state culture of palm kernel cake
    Iluyemi FB, Hanafi MM, Radziah O, Kamarudin MS
    Bioresour Technol, 2006 Feb;97(3):477-82.
    PMID: 16216731
    Palm kernel cake (PKC), an agro-industrial by-product used extensively in the animal feed industry, has limited use in fish feeds due to its high fiber and low protein contents. In this study, PKC was processed under solid state culture conditions with five fungal strains and the effect of this fungal culturing on the amino acid, fatty acid, cellulose and hemicellulose fractions was evaluated. Fungal strains used were Sclerotium rolfsii, Trichoderma harzianum, Trichoderma longiobrachiatum, Trichoderma koninggi and Aspergillus niger. Fungal growth was carried out at 50% moisture level and 1% inoculum level for 7 days. A significant increase in protein content from 18.76% to 32.79% was obtained by growing T. longibrachiatum on PKC. Cellulose level decreased significantly from 28.31% to 12.11% for PKC cultured with T. longibrachiatum, and hemicellulose from 37.03% to 19.01% for PKC cultured with A. niger. Fungal culturing of PKC brought about an increase in the level of unsaturated- and a decrease in the level of the saturated-fatty acids.
    Matched MeSH terms: Aspergillus niger/genetics; Aspergillus niger/growth & development; Aspergillus niger/metabolism
  8. Fulltext Partial Purification and Characterisation of Pectinase Produced by Aspergillus niger LFP-1 Grown on Pomelo Peels as a Substrate
    Jalil MTM, Ibrahim D
    Trop Life Sci Res, 2021 Mar;32(1):1-22.
    PMID: 33936548 DOI: 10.21315/tlsr2021.32.1.1
    In the present study, pectinase was produced by local fungal isolate, Aspergillus niger LFP-1 grown on pomelo peels as a sole carbon source under solid-state fermentation (SSF). The purification process begins with the concentration of crude enzyme using ammonium sulfate precipitation and followed by purification using anion-exchange column chromatography (DEAE-Sephadex) and subsequently using gel filtration column chromatography (Sephadex G-100). On the other hand, the molecular weight of the purified enzyme was determined through SDS-PAGE. The findings revealed the crude enzyme was purified up to 75.89 folds with a specific activity of 61.54 U/mg and the final yield obtained was 0.01%. The molecular mass of the purified pectinase was 48 kDa. The optimum pH and temperature were 3.5 and 50°C, respectively. This enzyme was stable at a range of pH 3.5 to 4.5 and a relatively high temperature (40°C-50°C) for 100 min. The Km and Vmax were found to be 3.89 mg/mL and 1701 U/mg, respectively. Meanwhile, pectin from citrus fruit and the metal ion (Co2+) were the best substrate and inducer to enhance pectinase yield, respectively.
    Matched MeSH terms: Aspergillus niger
  9. Effects of Clitoria ternatea leaf extract on growth and morphogenesis of Aspergillus niger
    Kamilla L, Mansor SM, Ramanathan S, Sasidharan S
    Microsc Microanal, 2009 Aug;15(4):366-72.
    PMID: 19575837 DOI: 10.1017/S1431927609090783
    Clitoria ternatea is known for its antimicrobial activity but the antifungal effects of leaf extract on growth and morphogenesis of Aspergillus niger have not been observed. The extract showed a favorable antifungal activity against A. niger with a minimum inhibition concentration 0.8 mg/mL and minimum fungicidal concentration 1.6 mg/mL, respectively. The leaf extract exhibited considerable antifungal activity against filamentous fungi in a dose-dependent manner with 0.4 mg/mL IC50 value on hyphal growth of A. niger. The main changes observed under scanning electron microscopy after C. ternatea extract treatment were loss of cytoplasm in fungal hyphae and the hyphal wall and its diameter became markedly thinner, distorted, and resulted in cell wall disruption. In addition, conidiophore alterations were also observed when A. niger was treated with C. ternatea leaf extract.
    Matched MeSH terms: Aspergillus niger/cytology; Aspergillus niger/drug effects*; Aspergillus niger/growth & development
  10. Fulltext Fungistatic effect of hydroxychloroquine, lessons from a case
    Keshavarzi F
    Med Mycol Case Rep, 2016 Sep;13:17-18.
    PMID: 27709021
    A 50-year-old male was prescribed with hydroxychloroquine (HCQ) after osteoarthritis was diagnosed. He had an old nail infection of Aspergillus niger. A remarkable improvement of the symptoms of fungal nail infection was seen after about four weeks of treatment with HCQ. It was very hard to detect the symptoms in the end of the second month of the treatment, both in the finger and toe nails. The symptoms were clearly recurred after HCQ was discontinued.
    Matched MeSH terms: Aspergillus niger
  11. Bioconversion of oil palm frond by Aspergillus niger to enhances it's fermentable sugar production
    Lim SH, Ibrahim D
    Pak J Biol Sci, 2013 Sep 15;16(18):920-6.
    PMID: 24502148
    The aim of this study was to develop an economical bioprocess to produce the fermentable sugars at laboratory scales Using Oil Palm Frond (OPF) as substrate in Solid State Fermentation (SSF). OPF waste generated by oil palm plantations is a major problem in terms of waste management. However, this lignocellulosic waste material is a cheap source of cellulose. We used OPF as substrate to produce fermentable sugars. The high content of cellulose in OPF promises the high fermentable sugars production in SSF. Saccharification of OPF waste by A. niger USMAI1 generates fermentable sugars and was evaluated through a solid state fermentation. Physical parameters, e.g., inoculum size, initial substrate moisture, initial pH, incubation temperature and the size of substrate were optimized to obtain the maximum fermentable sugars from oil palm fronds. Up to 77 mg of fermentable sugars per gram substrate was produced under the optimal physical parameter conditions. Lower productivity of fermentable sugars, 32 mg fermentable sugars per gram substrate was obtained under non optimized conditions. The results indicated that about 140.6% increase in fermentable sugar production after optimization of the physical parameters. Glucose was the major end component amongst the fermentable sugars obtained. This study indicated that under optimum physical parameter conditions, the OPF waste can be utilized to produce fermentable sugars which then convert into other products such as alcohol.
    Matched MeSH terms: Aspergillus niger/growth & development; Aspergillus niger/metabolism*
  12. Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential
    Ling Onn M, Teen Lim P, Aazani Mujahid, Proksch P, Müller M
    Sains Malaysiana, 2016;45:1063-1071.
    Endophytic fungi provide protection to their host plant and the fungi often produce antimicrobial compounds to aid the host
    fighting off pathogens. These bioactive compounds were secondary metabolites which were often produced as waste- or
    by-products. In the present study, endophytic fungi isolated from mangrove plants and soils were characterized and their
    antimicrobial production and bioremediation potential of heavy metals copper (Cu) and zinc (Zn) were assessed. Twelve
    (12) isolated and identified endophytic fungi belonged to seven species; Penicillium, Curvularia, Diaporthe, Aspergillus,
    Guignardia, Neusartorya and Eupenicillium. Antimicrobial activities of these 12 fungal endophytes were tested against
    Gram negative bacteria; Bacillus subtilis, Staphylococcus aureus, Gram positive bacteria; Escherichia coli and fungi;
    Candida albicans and Aspergillus niger among others. Two isolates (related to Guignardia sp. and Neusartoya sp.) showed
    strong antimicrobial (and antifungal) activity whereas the rest showed no activity. Compounds were isolated from both
    isolates and screened using HPLC. Both isolates displayed chemically very interesting chromatograms as they possessed a
    high diversity of basic chemical structures and peaks over a wide range of polarities, with structures similar to Trimeric
    catechin and Helenalin among others. For bioremediation assessment, the results showed maximum biosorption capacity
    for two isolates related to Curvularia sp. and Neusartorya sp., with the former removing 25 mg Cu/g biomass and the
    latter removing 24 mg Zn/g biomass. Our results indicated the potential of mangrove endophytic fungi in producing
    bioactive compounds and also highlighted their potential for the treatment of heavy metal-contaminated wastewater.
    Matched MeSH terms: Aspergillus niger
  13. Use of fungi to improve bioconversion of activated sludge
    Mannan S, Fakhru'l-Razi A, Alam MZ
    Water Res, 2005 Aug;39(13):2935-43.
    PMID: 16000208
    The present study was designed to evaluate the potential of microbial adaptation and its affinity to biodegradation as well as bioconversion of soluble/insoluble (organic) substances of domestic wastewater treatment plant (DWTP) sludge (activated domestic sludge) under natural/non-sterilized conditions. The two filamentous fungi, Penicillium corylophilum (WWZP1003) and Aspergillus niger (SCahmA103) were used to achieve the objectives. It was observed that P. corylophilum (WWZP1003) was the better strain compared to A. niger (SCahmA103) for the bioconversion of domestic activated sludge through adaptation. The visual observation in plate culture showed that about 95-98% of cultured microbes (P. corylophilum and A. niger) dominated in treated sludge after 2 days of treatment. In this study, it was also found that the P. corylophilum was capable of removing 94.40% of COD and 98.95% of turbidity of filtrate with minimum dose of inoculum of 10% v/v in DWTP sludge (1% w/w). The pH level was lower (acidic condition) in the fungal treatment and maximum reduction of COD and turbidity was observed (at lower pH). The results for specific resistance to filtration (SRF) showed that the fungi played a great role in enhancing the dewaterability and filterability. In particular, the strain Penicillium had a more significant capability (than A. niger) of reducing 93.20% of SRF compared to the uninoculated sample. Effective results were observed by using fungal inoculum after 2 days of treatment. The developed LSB process is a new biotechnological approach for sludge management strategy.
    Matched MeSH terms: Aspergillus niger/metabolism*
  14. Synthesis, Characterization, Antimicrobial and Anticancer Studies of Metal Complexes of 2-methoxy-4-((3-methylpyridin-2-ylimino)methyl)phenol
    Mishra A, Tahlan S, Ramasamy K, Lim SM, Shah SAA, Narasimhan B
    Mini Rev Med Chem, 2020;20(13):1311-1317.
    PMID: 32368977 DOI: 10.2174/1389557520666200505124125
    BACKGROUND: Being derived from primary amine and aromatic aldehyde, Schiff base and their complexes have an imperative role in the improvement of inorganic chemistry, which are broadly studied as coordination compounds and are gradually becoming more important in biochemical and analytical applications.

    METHODS: They have also been used for antibacterial, antifungal, anticancer, antitubercular activities. Novel synthesised Schiff's base 2-methoxy-4-((3-methylpyridin-2-ylimino)methyl)phenol (SB) and its metal complexes (Zn[II], Cu[II], Co[II] and Ni[II]) were characterised by UV, IR and NMR spectroscopy. Formation of the Schiff base and the metal (Zn[II], Cu[II], Co[II] and Ni[II]) chelates was supported by spectral and analytical data. The ligand and metal complexes have been screened for their antibacterial activity against Staphylococcus aureus, Salmonella typhi, Escherichia coli, Klebsiella pneumoniae and antifungal activity against the fungi Candida albicans and Aspergillus niger. Further, the synthesised compounds were also screened for antiproliferative activity against the human colorectal carcinoma (HCT116) cell line using the Sulforhodamine B assay.

    RESULT: Metal complexes formed were found to enhance the potency of the Schiff base due to coordination with a copper complex, showing better activity than others.

    CONCLUSION: Copper complex was observed to be more potent than other complexes against all the pathogenic microbes and cancer cell line (HCT116).

    Matched MeSH terms: Aspergillus niger
  15. Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization
    Mohamad SN, Ramanan RN, Mohamad R, Ariff AB
    N Biotechnol, 2011 Feb 28;28(2):146-52.
    PMID: 20970530 DOI: 10.1016/j.nbt.2010.10.008
    The effect of different carbon and nitrogen sources on the production of mannan-degrading enzymes, focussing on β-mannanase, by Aspergillus niger was investigated using shake flask culture. The β-mannanase activity obtained during growth of A. niger on guar gum (GG, 1495 nkat mL(-1)) was much higher than those observed on other carbon substrates, locust bean gum (1148 nkat mL(-1)), α-cellulose (10.7 nkat mL(-1)), glucose (8.8 nkat mL(-1)) and carboxymethylcellulose (4.6 nkat mL(-1)). For fermentation using GG as a carbon source, bacteriological peptone gave the highest β-mannanase activity (1744 nkat mL(-1)) followed by peptone from meat (1168 nkat mL(-1)), yeast extract (817 nkat mL(-1)), ammonium sulphate (241 nkat mL(-1)), ammonium nitrate (113 nkat mL(-1)) and ammonium chloride (99 nkat mL(-1)) when used as a nitrogen source. The composition of bacteriological peptone and initial pH of the medium were further optimized using response surface methodology (RSM). Medium consisted of 21.3 g L(-1) GG and 57 g L(-1) peptone with initial culture pH of 5.5 was optimum for β-mannanase production (2063 nkat mL(-1)) by A. niger. The β-mannanase production obtained in this study using A. niger was significantly higher than those reported in the literature.
    Matched MeSH terms: Aspergillus niger/enzymology*
  16. Subtopic: Advances in water and wastewater treatment harvesting of Chlorella sp. microalgae using Aspergillus niger as bio-flocculant for aquaculture wastewater treatment
    Mohd Nasir N, Mohd Yunos FH, Wan Jusoh HH, Mohammad A, Lam SS, Jusoh A
    J Environ Manage, 2019 Nov 01;249:109373.
    PMID: 31415924 DOI: 10.1016/j.jenvman.2019.109373
    Microalgae have been increasingly used to generate biofuel, thus a sustainable technique should be implemented to harvest the biomass to ensure its existence in the environment. Aspergillus niger was used as bio-flocculant to harvest microalgae from aquaculture wastewater via flocculation technique over a range of pH and mixing rate. The bio-flocculant showed ability to adapt at a wide range of pH from 3.0 to 9.0 and at a mixing rate of 100-150 rpm, producing a harvesting efficiency of higher than 90%. The treated water possessed low concentration of chlorophyll-a (0.3-0.6 mg L-1) and cell density (2 × 106-3 × 106 cell mL-1). These indicate that Aspergillus niger is a promising bio-flocculant to be used in harvesting microalgae, thus promoting the use of flocculation as a green technology in aquaculture wastewater treatment.
    Matched MeSH terms: Aspergillus niger
  17. Novel Antifungal Peptides Produced by Leuconostoc mesenteroides DU15 Effectively Inhibit Growth of Aspergillus niger
    Muhialdin BJ, Hassan Z, Abu Bakar F, Algboory HL, Saari N
    J Food Sci, 2015 May;80(5):M1026-30.
    PMID: 25847317 DOI: 10.1111/1750-3841.12844
    The ability of Leuconostoc mesenteroides DU15 to produce antifungal peptides that inhibit growth of Aspergillus niger was evaluated under optimum growth conditions of 30 °C for 48 h. The cell-free supernatant showed inhibitory activity against A. niger. Five novel peptides were isolated with the sequences GPFPL, YVPLF, LLHGVPLP, GPFPLEMTLGPT, and TVYPFPGPL as identified by de novo sequencing using PEAKS 6 software. Peptide LLHGVPLP was the only positively charged (cationic peptides) and peptide GPFPLEMTLGPT negatively charged (anionic), whereas the rest are neutral. The identified peptides had high hydrophobicity ratio and low molecular weights with amino acids sequences ranging from 5 to 12 residues. The mode of action of these peptides is observed under the scanning electron microscope and is due to cell lysis of fungi. This work reveals the potential of peptides from L. mesenteroides DU15 as natural antifungal preservatives in inhibiting the growth of A. niger that is implicated to the spoilage during storage.
    Matched MeSH terms: Aspergillus niger/drug effects*; Aspergillus niger/growth & development*
  18. Fulltext LIPASE PRODUCTION FROM SOLID STATE FERMENTATION OF COPRA WASTE ASSOCIATED FUNGUS Aspergillus niger
    NURUL NAZIRA ZULKIFLI, NAZAITULSHILA RASIT
    UMT Journal of Undergraduate Research, 2020;2(2):33-40.
    MyJurnal
    Lipases are enzyme with versatile industrial applications can be produced by the solid-state fermentation (SSF) method and is an economical alternative for enzyme production assisted by fungus. In Malaysia, 5 million of copra waste were generated annually. Large amount of copra waste produced will cause an increasing amount of the waste dumped to the landfill. Copra waste is one of the potential substrates to produce lipase enzyme through SSF. Thus, the aim of this study is to optimize the lipase production by SSF associated by Aspergillus niger using the 23 full factorial design approach. In this study the factors affecting parameters that involved in the production of lipase enzyme such as temperature (25˚ and 35˚), substrates concentration (40% and 60%) and inoculum size of Aspergillus niger (1 and 9 petri dish) were determined. The maximum production of lipase was obtained after 120-hour incubation in SSF. The optimum condition for inoculum size of Aspergillus niger was 9 plates, 30°C of incubation temperature and 60 % moisture contents. The range of the concentration of lipase enzyme produced varied from 105 U/ml to 170 U/ml. When applied to the wastewater treatment, the reducing percentage of fat, oil and grease (FOG) in food processing wastewater is reduced from 219.4925mg/l to 169.467mg/l accounted to the amount of 34 % FOG removal. Lipase produced using copra waste as a substrate using SSF has the potential value to be developed in the future for various industry including wastewater treatment industry.
    Matched MeSH terms: Aspergillus niger
  19. Treatment of African catfish, Clarias gariepinus wastewater utilizing phytoremediation of microalgae, Chlorella sp. with Aspergillus niger bio-harvesting
    Nasir NM, Bakar NS, Lananan F, Abdul Hamid SH, Lam SS, Jusoh A
    Bioresour Technol, 2015 Aug;190:492-8.
    PMID: 25791330 DOI: 10.1016/j.biortech.2015.03.023
    This study focuses on the evaluation of the performance of Chlorella sp. in removing nutrient in aquaculture wastewater and its correlation with the kinetic growth of Chlorella sp. The treatment was applied with various Chlorella sp. inoculation dosage ranging from 0% to 60% (v/v) of wastewater. The optimum inoculation dosage was recorded at 30% (v/v) with effluent concentration of ammonia and orthophosphate recording at 0.012mgL(-1) and 0.647mgL(-1), respectively on Day 11. The optimum dosage for bio-flocculation process was obtained at 30mgL(-1) of Aspergillus niger with a harvesting efficiency of 97%. This type of development of phytoremediation with continuous bio-harvesting could promote the use of sustainable green technology for effective wastewater treatment.
    Matched MeSH terms: Aspergillus niger/metabolism*
  20. Onychomycosis in Malaysia
    Ng KP, Saw TL, Madasamy M, Soo Hoo T
    Mycopathologia, 1999;147(1):29-32.
    PMID: 10872513
    The common etiological agents of onychomycosis are dermatophytes, molds and yeasts. A mycological nail investigation of onychomycosis using direct microscopy and culture was conducted by the Mycology Unit, Department of Medical Microbiology, University of Malaya from March 1996 to November 1998. The study involved 878 nail clippings or subungal scrapings from subjects with onychomycosis. On direct microscopy examination, 50% of the specimens were negative for fungal elements. On culture, 373 specimens had no growth; bacteria were isolated from 15 nail specimens. Among the 490 specimens with positive fungal cultures, 177 (36.1%) were dermatophytes, 173 (35.5%) were molds and 130 (26.5%) were Candida. There were 2% (10/490) mixed infections of molds, yeasts and dermatophytes. Trichophyton rubrum (115/177) and Trichophyton mentagrophytes (59/177) were the main dermatophytes isolated. The molds isolated were predominantly Aspergillus niger (61/173), Aspergillus nidulans (30/173), Hendersonula toruloidea (26/173) and Fusarium species (16/173). 96.9% of the Candida species identified were Candida albicans.
    Matched MeSH terms: Aspergillus niger/growth & development; Aspergillus niger/pathogenicity
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