Displaying publications 21 - 40 of 888 in total

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  1. Investigating the Cytoprotective Effect of Syzygium aromaticum and Elettaria cardamomum in Arsenic-Induced Toxicity in the HCT-116 Cell Model: A Luminesce-Based Study
    Nelson VK, Krishnan SKR, Dera AA, Ali SP, Begum MY, Burle GSR, et al.
    Luminescence, 2025 Mar;40(3):e70119.
    PMID: 40045745 DOI: 10.1002/bio.70119
    Arsenic is a highly menacing environmental pollutant that substantially affects the quality of life, even in traces. It develops various diseases through increasing intracellular ROS that interacts with vital organs. Chelation therapy is currently recommended for arsenic toxicity. However, these treatment options are not specific and only target symptoms. Besides, they also show toxic effects and irreversibly damage crucial organs. Hence, there is an urgent need for a potential and safer therapeutic strategy that can prevent arsenic toxicity. Therefore, in this study, we have investigated SA and EC individually and in combination against arsenic toxicity in HCT-116 cells. The results obtained from various experiments revealed that both plant materials comparably inhibited arsenic-induced cellular damage by increasing the antioxidant function. Conversely, combining both plants showed even better cytoprotection against arsenic toxicity. The FT-IR and HPLC analysis of the active fractions revealed the presence of polyphenol compounds (ferulic acid and gallic acid) responsible for the pharmacological effect. This finding is unique because no cytoprotective impact has been reported yet on this combination.
    Matched MeSH terms: Cell Survival/drug effects
  2. Fulltext CD44-Receptors-Mediated Multiprong Targeting Strategy Against Breast Cancer and Tumor-Associated Macrophages: Design, Optimization, Characterization, and Cytologic Evaluation
    Hussain Z, Abdulrahim Abdul Moti L, Jagal J, Thu HE, Khan S, Kazi M
    Int J Nanomedicine, 2025;20:991-1020.
    PMID: 39881963 DOI: 10.2147/IJN.S480553
    INTRODUCTION: Owing to its high prevalence, colossal potential of chemoresistance, metastasis, and relapse, breast cancer (BC) is the second leading cause of cancer-related fatalities in women. Several treatments (eg, chemotherapy, surgery, radiations, hormonal therapy, etc.) are conventionally prescribed for the treatment of BC; however, these are associated with serious systemic aftermaths. In this research, we aimed to design a multiprong targeting strategy for concurrent action against different phenotypes of BC (MCF-7 and SK-BR-3) and tumor-associated macrophages (TAMs) for relapse-free treatment of BC.

    METHODS: Paclitaxel (PTX) and tamoxifen (TMX) co-loaded chitosan (CS) nanoparticles (NPs) were prepared using the ionic-gelation method and optimized using the Design Expert® software by controlling different material attributes. For selective targeting through CD44-receptors that are heavily expressed on the BC cells and TAMs, the fabricated NPs (PTX-TMX-CS-NPs) were functionalized with hyaluronic acid (HA) as a targeting ligand.

    RESULTS: The optimized HA-PTX-TMX-CS-NPs exhibited desired physicochemical properties (PS ~230 nm, PDI 0.30, zeta potential ~21.5 mV), smooth spherical morphology, high encapsulation efficiency (PTX ~72% and TMX ~97%), good colloidal stability, and biphasic release kinetics. Moreover, the lowest cell viability depicted in MCF-7 (~25%), SK-BR-3 (~20%), and RAW 264.7 cells (~20%), induction of apoptosis, cell cycle arrest, enhanced cell internalization, and alleviation of MCF-7 and SK-BR-3 migration proved the superior anticancer potential of HA-PTX-TMX-CS-NPs compared to unfunctionalized NPs and other control medicines.

    CONCLUSION: HA-functionalization of NPs is a promising multiprong strategy for CD44-receptors-mediated targeting of BC cells and TAMs to mitigate the progression, metastasis, and relapse in the BC.

    Matched MeSH terms: Cell Survival/drug effects
  3. Resveratrol-Induced Modulation of Key Genes and DNA Fragmentation in Chronic Myeloid Leukemia Cells
    Alhawamdeh L, Almajali B, Atoom AM, Saad HKM, Madi R, Al-Jamal HAN
    Asian Pac J Cancer Prev, 2025 Mar 01;26(3):905-911.
    PMID: 40156407 DOI: 10.31557/APJCP.2025.26.3.905
    OBJECTIVE: Chronic myeloid leukemia (CML) is a hematologic malignancy characterized by the BCR-ABL1 fusion gene, which drives the uncontrolled proliferation of myeloid cells. Despite advancements in treatment, resistance to conventional therapies remains a significant challenge. Resveratrol, a natural polyphenol, has garnered attention for its potential therapeutic properties, including its ability to modulate key genes and induce apoptosis in cancer cells. This study investigated the effects of resveratrol on apoptosis, cell cycle regulation, and DNA fragmentation in CML cells.

    METHODS: K562 CML cells were treated with resveratrol, and their effects were analyzed through CCK-8 assay for cell viability, TUNEL assay for DNA fragmentation, and real-time PCR for gene expression. Key apoptotic genes (BCL-2, AIF, BAX) were assessed alongside survival-related genes (CASP3, PGC1α, Cyclin-D1, p53) to evaluate resveratrol's anti-proliferative and pro-apoptotic potential.

    RESULT: Resveratrol exhibited a time-dependent reduction in K562 cell viability, with IC₅₀ values decreasing from 282.2 µM at 24 hours to 107.1 µM and 102.4 µM at 48 and 72 hours, respectively. Apoptotic activity, assessed via the TUNEL assay, revealed significant DNA fragmentation in 55 ± 5% of treated cells, while control cells showed no fragmentation. Gene expression analysis demonstrated upregulation of pro-apoptotic genes, including BCL-2, AIF (p < 0.05), BAX (p < 0.01), and VDAC1 (4.5-fold, p < 0.001). Conversely, genes linked to cell survival and metabolism, such as CASP3, PGC1α, NDUFA9, Cyclin-D1, and p53, were slightly downregulated (p < 0.05), highlighting resveratrol's dual role in promoting apoptosis and inhibiting cell survival.

    CONCLUSION: These findings suggest that resveratrol exerts anti-proliferative and pro-apoptotic effects in CML cells by modulating key genes and induction of DNA fragmentation, highlighting its potential as a therapeutic agent for CML treatment.

    Matched MeSH terms: Cell Survival/drug effects
  4. Dual anthelmintic and hepatoprotective potential of Populus tremula bark polysaccharide complex in vitro
    Perina EA, Buyko EE, Krivoshchekov SV, Efimova LV, Diksas EB, Kaidash OA, et al.
    Trop Biomed, 2026 Sep 01;43(3):330-341.
    PMID: 42960653 DOI: 10.47665/tb.43.3.013
    Opisthorchiasis, caused by the liver fluke Opisthorchis felineus, remains a significant public health problem in Western Siberia and other parts of Eurasia. The present study investigated the anthelmintic and hepatoprotective properties of a polysaccharide complex (PC) extracted from Populus tremula bark. Anthelmintic activity was assessed in vitro against O. felineus worms using motility scores, MTS viability assay, and propidium iodide (PI) staining. Hepatoprotective potential was evaluated in HepG2 cells using lipopolysaccharide/interferon-g (LPS/IFN-g) induced inflammation and free fatty acid (FFA) induced lipotoxicity models; reactive oxygen species (ROS), reduced glutathione (GSH), and intracellular lipid content were measured fluorimetrically. Antioxidant activity was further characterised by DPPH radical scavenging and ferrous ion chelating assays. PC significantly reduced worm motility and viability (calculated motility index decreased by approximately 45%, from 3.80 to 2.10), and induced focal tegumental PI accumulation, consistent with surface damage. In the LPS/IFN-g model, PC at 125 µg/mL decreased ROS by 14% (p=0.001) and partially restored GSH content by 25% (p=0.016). In FFA treated cells, the same concentration attenuated ROS by 15.5% (p=0.001) and reduced lipid accumulation by 22.8% (p=0.008). PC did not affect HepG2 viability. The complex demonstrated modest DPPH radicalscavenging activity (33.6% at 200 µg/mL) and a pronounced Fe2+ chelating capacity (24.70±2.63 mg EDTA equivalents/g). These first initial in vitro findings identify P. tremula bark PC as a candidate dualaction agent for treating of opisthorchiasis, combining direct anthelmintic activity with multi-targeted hepatoprotective effects that warrant confirmation in further mechanistic and in vivo studies.
    Matched MeSH terms: Cell Survival/drug effects
  5. The cytotoxicity of breast cancer mcf-7 cell line treated with different wavelength of low-level laser
    Ibrahim HA, Suardi N, Khaniabadi PM, Zulbaharin SFM, Taggo A
    Lasers Med Sci, 2024 Sep 23;39(1):238.
    PMID: 39307856 DOI: 10.1007/s10103-024-04187-9
    Breast cancer remains a significant global health challenge, spurring ongoing investigations into innovative treatment approaches. Low-level laser therapy (LLLT) has emerged as a promising non-invasive therapeutic avenue of interest. This research delves into the impact of LLLT on the cytotoxicity of the MCF-7 breast cancer cell line, employing lasers emitting various wavelengths. The objective is to assess whether diverse LLLT wavelengths elicit disparate cytotoxic responses, shedding light on LLLT's potential as a targeted breast cancer treatment. MCF-7 cell cultures were subjected to lasers of varying wavelengths, including blue (473 nm), red (660 nm), and near-infrared (780 nm). Each wavelength was delivered at four different power levels: 10, 25, 45, and 65 mW, with exposure durations of 60, 300, 600, and 900 s. Cellular responses, encompassing factors such as cell viability, and cytotoxicity were assessed using WST-1 assays technique. Statistical analysis was performed to discern the wavelength-specific impacts of low-level laser therapy (LLLT) on MCF-7 cells. The study revealed that the blue laser had the least noticeable adverse impact on MCF-7 breast cancer cell lines, leading to the highest cell survival rate of 107.62% after 24 h. The most severe toxicity occurred when the laser was used at 45 mW for 900 s, resulting in cell viability ranging from 81.85% to 107.62%. As for cell viability after exposure to the red laser, the mildest harmful effect was observed at 45 mW power for 60 s, resulting in a cell survival rate of 147.62%. Conversely, the most significant toxic response occurred at 10 mW power for 60 s, resulting in a cell viability of 91.56%. In contrast, when employing infrared laser irradiation, the least substantial cytotoxic effect on MCF-7 cells was observed at 10 mW power for 600 s, resulting in the highest cell viability of 109.37% after 24 h. The most pronounced cytotoxic effect was observed by infrared laser (780 nm) at 25 mW power for 900 s, leading to the lowest viability of 32.53%.
    Matched MeSH terms: Cell Survival/radiation effects
  6. Structural insights and cytotoxicity evaluation of benz[e]indole pyrazolyl-substituted amides
    Ramle AQ, Chan NNMY, Ng MP, Tan CH, Sim KS, Tiekink ERT, et al.
    Mol Divers, 2024 Jun;28(3):1363-1376.
    PMID: 37278911 DOI: 10.1007/s11030-023-10662-2
    Five new compounds of benz[e]indole pyrazolyl-substituted amides (2a-e) were synthesised in low to good yields via the direct amide-coupling reaction between a pyrazolyl derivative containing a carboxylic acid and several amine substrates. The molecular structures were determined by various spectroscopic methods, such as NMR (1H, 13C and 19F), FT-IR and high-resolution mass spectrometry (HRMS). X-ray crystallographic analysis on the 4-fluorobenzyl derivative (2d) reveals the amide-O atom to reside to the opposite side of the molecule to the pyrazolyl-N and pyrrolyl-N atoms; in the molecular packing, helical chains feature amide-N‒H⋯N(pyrrolyl) hydrogen bonds. Density-functional theory (DFT) at the geometry-optimisation B3LYP/6-31G(d) level on the full series shows general agreement with the experimental structures. While the LUMO in each case is spread over the benz[e]indole pyrazolyl moiety, the HOMO spreads over the halogenated benzo-substituted amide moieties or is localised near the benz[e]indole pyrazolyl moieties. The MTT assay showed that 2e, exhibited the highest toxicity against a human colorectal carcinoma (HCT 116 cell line) without appreciable toxicity towards the normal human colon fibroblast (CCD-18Co cell line). Based on molecular docking calculations, the probable cytotoxic mechanism of 2e is through the DNA minor groove binding.
    Matched MeSH terms: Cell Survival/drug effects
  7. Antioxidant and anticancer activities of hesperetin and its novel formulations in KB cells
    Chauhan A, Salwa, Shedgaonkar GG, Kumar L, Karmakar A, Khajuria S, et al.
    Naunyn Schmiedebergs Arch Pharmacol, 2025 May;398(5):5213-5236.
    PMID: 39531045 DOI: 10.1007/s00210-024-03581-y
    This study aimed to formulate the hesperetin nanostructured lipid carriers (NLCs) containing oro-mucosal gel for its activity assessment on the KB cell line. NLCs were prepared with glyceryl monostearate, oleic acid, and lecithin using a modified constant-temperature emulsification technique. The particle size analysis, in vitro drug release studies, etc., of prepared NLCs were evaluated. The formulated gels were analyzed with respect to spreadability, extrudability, swelling index, texture analysis, etc. The particle size, polydispersity index, zeta potential, and drug entrapment of nanocarriers were recorded to be 221.733 ± 61.536 nm, 0.381 ± 0.091, - 51.433 ± 4.143 mV, and 89.29%, respectively. The optimized NLCs in 24 h released 87.14 ± 6.62% of the drug. The round shape of NLCs was noticed with scanning electron microscopy. The pH, spreadability, extrudability, swelling index, content uniformity, and drug release studies of hesperetin NLCs-containing gel (HNG) were found to be 6.81 ± 0.04, 2.49 ± 0.04 cm.mg/s, 539.04 ± 32.88 g/cm2, 4.27 ± 0.47, 107.98 ± 1.93%, and 90.17 ± 6.67% (in 48 h), respectively. The developed formulations showed promising in vitro anticancer and antioxidant activities. HNP results authorize that the formulation may be beneficial for the treatment of oral cancer.
    Matched MeSH terms: Cell Survival/drug effects
  8. Fulltext Cytotoxic effect of commonly used food dyes on human Hepatoma cell line, HepG2
    Lye, H.M., Chiew, J.C., Siddique, M.M.
    International Food Research Journal, 2018;25(4):1457-1463.
    MyJurnal
    The increasing and widespread use of synthetic food dyes raises health concerns and earlier reports suggest that certain food dyes might be harmful for human health. In this study, we have investigated the effect of three commonly used food dyes on human liver cell line, HepG2. Our findings suggest that these experimental food dyes significantly affect cell viability and this effect can be worsen in hyperglycemic condition. Accumulation of cellular fat was significantly higher in presence of these dyes. Expression pattern of the gene involved in regulating apoptosis suggests that that the observed cell death could be attributed to the activation of apoptotic pathway. These findings suggest that these experimental dyes might exert synergistic toxicity in hyperglycemia that need to be confirmed using suitable in vivo models.
    Matched MeSH terms: Cell Survival
  9. Fulltext In Vitro and In Vivo Biological Assessments of 3D-Bioprinted Scaffolds for Dental Applications
    Mohd N, Razali M, Fauzi MB, Abu Kasim NH
    Int J Mol Sci, 2023 Aug 17;24(16).
    PMID: 37629064 DOI: 10.3390/ijms241612881
    Three-dimensional (3D) bioprinting is a unique combination of technological advances in 3D printing and tissue engineering. It has emerged as a promising approach to address the dilemma in current dental treatments faced by clinicians in order to repair or replace injured and diseased tissues. The exploration of 3D bioprinting technology provides high reproducibility and precise control of the bioink containing the desired cells and biomaterial over the architectural and dimensional features of the scaffolds in fabricating functional tissue constructs that are specific to the patient treatment need. In recent years, the dental applications of different 3D bioprinting techniques, types of novel bioinks, and the types of cells used have been extensively explored. Most of the findings noted significant challenges compared to the non-biological 3D printing approach in constructing the bioscaffolds that mimic native tissues. Hence, this review focuses solely on the implementation of 3D bioprinting techniques and strategies based on cell-laden bioinks. It discusses the in vitro applications of 3D-bioprinted scaffolds on cell viabilities, cell functionalities, differentiation ability, and expression of the markers as well as the in vivo evaluations of the implanted bioscaffolds on the animal models for bone, periodontal, dentin, and pulp tissue regeneration. Finally, it outlines some perspectives for future developments in dental applications.
    Matched MeSH terms: Cell Survival
  10. Fulltext The radiobiological effects of He, C and Ne ions as a function of LET on various glioblastoma cell lines
    Chew MT, Bradley DA, Suzuki M, Matsufuji N, Murakami T, Jones B, et al.
    J Radiat Res, 2019 Mar 01;60(2):178-188.
    PMID: 30624699 DOI: 10.1093/jrr/rry099
    The effects of the charged ion species 4He, 12C and 20Ne on glioblastoma multiforme (GBM) T98G, U87 and LN18 cell lines were compared with the effects of 200 kVp X-rays (1.7 keV/μm). These cell lines have different genetic profiles. Individual GBM relative biological effectiveness (RBE) was estimated in two ways: the RBE10 at 10% survival fraction and the RBE2Gy after 2 Gy doses. The linear quadratic model radiosensitivity parameters α and β and the α/β ratio of each ion type were determined as a function of LET. Mono-energetic 4He, 12C and 20Ne ions were generated by the Heavy Ion Medical Accelerator at the National Institute of Radiological Sciences in Chiba, Japan. Colony-formation assays were used to evaluate the survival fractions. The LET of the various ions used ranged from 2.3 to 100 keV/μm (covering the depth-dose plateau region to clinically relevant LET at the Bragg peak). For U87 and LN18, the RBE10 increased with LET and peaked at 85 keV/μm, whereas T98G peaked at 100 keV/μm. All three GBM α parameters peaked at 100 keV/μm. There is a statistically significant difference between the three GBM RBE10 values, except at 100 keV/μm (P < 0.01), and a statistically significant difference between the α values of the GBM cell lines, except at 85 and 100 keV/μm. The biological response varied depending on the GBM cell lines and on the ions used.
    Matched MeSH terms: Cell Survival/drug effects; Cell Survival/radiation effects
  11. Neolamarckines A and B, new indole alkaloids from Neolamarckia cadamba
    Qureshi AK, Mukhtar MR, Hirasawa Y, Hosoya T, Nugroho AE, Morita H, et al.
    Chem Pharm Bull (Tokyo), 2011;59(2):291-3.
    PMID: 21297315
    Two new indole alkaloids, neolamarckines A and B (1, 2) were isolated from the leaves of Neolamarckia cadamba (Rubiaceae). Structural elucidation of 1 and 2 was performed by combination of 2D-NMR and circular dichroism (CD) spectra, and chemical correlations. Neolamarckine A (1) showed inhibition of inducible nitric oxide synthase (iNOS) dose dependently.
    Matched MeSH terms: Cell Survival/drug effects; Cell Survival/physiology
  12. Size-dependent effect of cystine/citric acid-capped confeito-like gold nanoparticles on cellular uptake and photothermal cancer therapy
    Saw WS, Ujihara M, Chong WY, Voon SH, Imae T, Kiew LV, et al.
    Colloids Surf B Biointerfaces, 2018 Jan 01;161:365-374.
    PMID: 29101882 DOI: 10.1016/j.colsurfb.2017.10.064
    Physiochemical changes, including size, are known to affect gold nanoparticle cellular internalization and treatment efficacy. Here, we report the effect of four sizes of cystine/citric acid-coated confeito-like gold nanoparticles (confeito-AuNPs) (30, 60, 80 and 100nm) on cellular uptake, intracellular localization and photothermal anticancer treatment efficiency in MDA-MB231 breast cancer cells. Cellular uptake is size dependent with the smallest size of confeito-AuNPs (30nm) having the highest cellular internalization via clathrin- and caveolae-mediated endocytosis. However, the other three sizes (60, 80 and 100nm) utilize clathrin-mediated endocytosis for cellular uptake. The intracellular localization of confeito-AuNPs is related to their endocytosis mechanism, where all sizes of confeito-AuNPs were localized highly in the lysosome and mitochondria, while confeito-AuNPs (30nm) gave the highest localization in the endoplasmic reticulum. Similarly, a size-dependent trend was also observed in in vitro photothermal treatment experiments, with the smallest confeito-AuNPs (30nm) giving the highest cell killing rate, whereas the largest size of confeito-AuNPs (100nm) displayed the lowest photothermal efficacy. Its desirable physicochemical characteristics, biocompatible nature and better photothermal efficacy will form the basis for further development of multifunctional confeito-AuNP-based nanotherapeutic applications.
    Matched MeSH terms: Cell Survival/drug effects; Cell Survival/radiation effects
  13. Fulltext Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells
    Al-Fahdawi MQ, Al-Doghachi FAJ, Abdullah QK, Hammad RT, Rasedee A, Ibrahim WN, et al.
    Biomed Pharmacother, 2021 Jun;138:111483.
    PMID: 33744756 DOI: 10.1016/j.biopha.2021.111483
    The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinum-doped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30-50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anti-cancer compound.
    Matched MeSH terms: Cell Survival/drug effects; Cell Survival/physiology
  14. Fulltext Bacteriostatic antimicrobial combination: antagonistic interaction between epsilon-viniferin and vancomycin against methicillin-resistant Staphylococcus aureus
    Basri DF, Xian LW, Abdul Shukor NI, Latip J
    Biomed Res Int, 2014;2014:461756.
    PMID: 24783205 DOI: 10.1155/2014/461756
    Stilbenoids have been considered as an alternative phytotherapeutic treatment against methicillin-resistant Staphylococcus aureus (MRSA) infection. The combined effect of ε-viniferin and johorenol A with the standard antibiotics, vancomycin and linezolid, was assessed against MRSA ATCC 33591 and HUKM clinical isolate. The minimum inhibitory concentration (MIC) value of the individual tested compounds and the fractional inhibitory concentration index (FICI) value of the combined agents were, respectively, determined using microbroth dilution test and microdilution checkerboard (MDC) method. Only synergistic outcome from checkerboard test will be substantiated for its rate of bacterial killing using time-kill assay. The MIC value of ε -viniferin against ATCC 33591 and johorenol A against both strains was 0.05 mg/mL whereas HUKM strain was susceptible to 0.1 mg/mL of ε-viniferin. MDC study showed that only combination between ε-viniferin and vancomycin was synergistic against ATCC 33591 (FICI 0.25) and HUKM (FICI 0.19). All the other combinations (ε-viniferin-linezolid, johorenol A-vancomycin, and johorenol A-linezolid) were either indifferent or additive against both strains. However, despite the FICI value showing synergistic effect for ε-viniferin-vancomycin, TKA analysis displayed antagonistic interaction with bacteriostatic action against both strains. As conclusion, ε-viniferin can be considered as a bacteriostatic stilbenoid as it antagonized the bactericidal activity of vancomycin. These findings therefore disputed previous report that ε-viniferin acted in synergism with vancomycin but revealed that it targets similar site in close proximity to vancomycin's action, possibly at the bacterial membrane protein. Hence, this combination has a huge potential to be further studied and developed as an alternative treatment in combating MRSA in future.
    Matched MeSH terms: Cell Survival/drug effects; Cell Survival/physiology
  15. Fulltext Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED)
    Nur Sazwi Nordin, Lokman Mohammad Isa, Syed Zahir Idid, Widya Lestari, Basma Ezzat Mustafa, Solachuddin Jauhari Arief Ikhwan, et al.
    International Medical Journal Malaysia, 2017;16(21):48-48.
    MyJurnal
    Flaxseeds offer a wide range of pharmacological properties including antioxidant,
    antibacterial and anticancer. However its effect on mesenchymal stem cells has not been
    elucidated. Thus, this study aimed to determine the effects of flaxseed crude extract on stem cell
    from human exfoliated deciduous teeth (SHED) in terms of cell viability, morphology and
    proliferation activity. (Copied from article).
    Matched MeSH terms: Cell Survival
  16. Fulltext Dose enhancement effects by different size of gold nanoparticles under irradiation of megavoltage photon beam
    Wan Nordiana W Abd Rahman, Raizulnasuha Ab Rashid, Mahfuzah Muhammad, Khairunisak Abdul Razak, Norhayati Dollah, Moshi Geso
    Jurnal Sains Nuklear Malaysia, 2018;30(2):23-29.
    MyJurnal
    Gold nanoparticles (AuNPs) have been extensively investigated as dose enhancement agent to increase the lethal dose to the tumours while minimizing dose to the normal tissue. Their intriguing properties and characteristics such as small size and shape provide favorable option in increasing radiotherapy therapeutic efficiency. In this study, the effects of AuNPs size on the dose enhancement effects irradiated under megavoltage photon beams were investigated. The study was conducted in-vitro on HeLa cells using AuNPs of 5 nm and 15 nm sizes. The cells samples were incubated with AuNPs and irradiated with photon beam of energy 6 MV and 10 MV at 100 cm SSD and 10 cm x 10 cm field size. Clonogenic assay were performed to observe the dose enhancement effects on cell survival. Dose enhancement factor (DEF) were extrapolated and evaluated from the cell survival curves. The results show that both sizes of AuNPs produce dose enhancement with the larger size AuNPs of 15 nm produce more dose enhancement compare to 5 nm AuNPs for 6 MV photon beam. Dose enhancements were observed for 10 MV photon beams but DEF for both sizes AuNPs shows no differences. In conclusion, larger size AuNPs produce higher dose enhancement compare to small size of AuNPs which conclude that nanoparticles size is important factor that need to be taken into account for AuNPs to be applied in radiotherapy.
    Matched MeSH terms: Cell Survival
  17. Fulltext Influence of out-of-field photon beam radiotherapy to the cancer cell survival
    Raizulnasuha Ab Rashid, Nurhikmah Azam, Norhayati Dollah, Wan Nordiana W Abd Rahman
    Jurnal Sains Nuklear Malaysia, 2018;30(2):47-56.
    MyJurnal
    The purpose of the study was to determine the effect of out-of-field photon beams radiotherapy to the cancer cell survival. In this study, HeLa and T24 cancer cells were irradiated with 6 MV and 10 MV photon beams in two different conditions, one with intercellular communication with the in-field cell and one without the communication. Cells survival was determined by clonogenic assay. In the presence of intercellular communication, the cell death was increased which indicate the presence of radiation induced bystander effects (RIBE). The effects were also dependent on the cell types and photon energy where the HeLa cells exhibit less survival compares to T24 cells and the effects were prominent at higher photon energy. This study demonstrates that the out-of-field cells in conjunction with RIBE plays important roles in the cells response towards megavoltage photon beam radiation therapy.
    Matched MeSH terms: Cell Survival
  18. Fulltext Cell viability in three ex vivo rat models of spinal cord injury
    Patar A, Dockery P, Howard L, McMahon SS
    J Anat, 2019 02;234(2):244-251.
    PMID: 30417349 DOI: 10.1111/joa.12909
    Spinal cord injury (SCI) is a devastating disorder that has a poor prognosis of recovery. Animal models of SCI are useful to understand the pathophysiology of SCI and the potential use of therapeutic strategies for human SCI. Ex vivo models of central nervous system (CNS) trauma, particularly mechanical trauma, have become important tools to complement in vivo models of injury in order to reproduce the sequelae of human CNS injury. Ex vivo organotypic slice cultures (OSCs) provide a reliable model platform for the study of cell dynamics and therapeutic intervention following SCI. In addition, these ex vivo models support the 3R concept of animal use in SCI research - replacement, reduction and refinement. Ex vivo models cannot be used to monitor functional recovery, nor do they have the intact blood supply of the in vivo model systems. However, the ex vivo models appear to reproduce many of the post traumatic events including acute and secondary injury mechanisms. Several well-established OSC models have been developed over the past few years for experimental spinal injuries ex vivo in order to understand the biological response to injury. In this study, we investigated cell viability in three ex vivo OSC models of SCI: stab injury, transection injury and contusion injury. Injury was inflicted in postnatal day 4 rat spinal cord slices. Stab injury was performed using a needle on transverse slices of spinal cord. Transection injury was performed on longitudinal slices of spinal cord using a double blade technique. Contusion injury was performed on longitudinal slices of spinal cord using an Infinite Horizon impactor device. At days 3 and 10 post-injury, viability was measured using dual staining for propidium iodide and fluorescein diacetate. In all ex vivo SCI models, the slices showed more live cells than dead cells over 10 days in culture, with higher cell viability in control slices compared with injured slices. Although no change in cell viability was observed between time-points in stab- and contusion-injured OSCs, a reduction in cell viability was observed over time in transection-injured OSCs. Taken together, ex vivo SCI models are a useful and reliable research tool that reduces the cost and time involved in carrying out animal studies. The use of OSC models provides a simple way to study the cellular consequences following SCI, and they can also be used to investigate potential therapeutics regimes for the treatment of SCI.
    Matched MeSH terms: Cell Survival
  19. Effect of storage conditions on maintaining anammox cell viability during starvation and recovery
    Ganesan S, Vadivelu VM
    Bioresour Technol, 2020 Jan;296:122341.
    PMID: 31711905 DOI: 10.1016/j.biortech.2019.122341
    Anammox bacteria can easily undergo starvation due to fluctuations in feed flowrate and concentration in wastewater treatment plants. In this study, we analyzed the effects of different types of storage conditions (presence of ammonium (Ra), nitrite (Rn), hydrazine (Rh), and no substrate (Rc)) in aiding the viability of anammox bacteria during starvation and recovery. After starvation, the bacteria were subjected to a 15-week recovery period. Anammox bacteria showed better results during starvation and recovery in Rh as compared to other conditions. Decay rate values obtained after starvation in Ra, Rn, Rh, and Rc were 0.032/day, 0.042/day, 0.019/day, and 0.037/day, respectively. Meanwhile, µmax values obtained in Rh, Ra, Rn, and Rc on the 15th week of recovery were 0.092, 0.075, 0.011, and 0.067 d-1, respectively. This indicated that the availability of hydrazine helps to reduce the mortality rate of anammox bacteria during starvation and enhances the recovery of anammox process.
    Matched MeSH terms: Cell Survival
  20. Inhibitory Activity of Extract, Fractions, and Compounds from Zingiber montanum Rhizomes on the Migration of Breast Cancer Cells
    Al-Amin M, Eltayeb NM, Hossain CF, Khairuddean M, Fazalul Rahiman SS, Salhimi SM
    Planta Med, 2020 Apr;86(6):387-394.
    PMID: 32168546 DOI: 10.1055/a-1129-7026
    Zingiber montanum rhizomes are traditionally used for the treatment of numerous human ailments. The present study was carried out to investigate the inhibitory activity of the crude extract, chromatographic fractions, and purified compounds from Z. montanum rhizomes on the migration of MDA-MB-231 cells. The effect of the extract on cell migration was investigated by a scratch assay, which showed significant inhibition in a concentration-dependent manner. Vacuum liquid chromatography on silica gel afforded four fractions (Frs. 1 - 4), which were tested on cell migration in the scratch assay. Frs. 1 and 2 showed the most significant inhibition of MDA-MB-231 cell migration. The effect of the most potent fraction (Fr. 2) was further confirmed in a transwell migration assay. The study of Frs. 1 and 2 by gelatin zymography showed significant inhibition of MMP-9 enzyme activity. Chromatographic separation of Frs. 1 and 2 afforded buddledone A (1: ), zerumbone (2: ), (2E,9E)-6-methoxy-2,9-humuradien-8-one (3: ), zerumbone epoxide (4: ), stigmasterol (5: ), and daucosterol (6: ). In a cell viability assay, compounds 1:  - 4: inhibited the viability of MDA-MB-231 cells in a concentration-dependent manner. The study of buddledone A (1: ) and zerumbone epoxide (4: ) on cell migration revealed that 4: significantly inhibited the migration of MDA-MB-231 cells in both scratch and transwell migration assays. The results of the present study may lead to further molecular studies behind the inhibitory activity of zerumbone epoxide (4: ) on cell migration and support the traditional use of Z. montanum rhizomes for the treatment of cancer.
    Matched MeSH terms: Cell Survival
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