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  1. Neo EX, Hasikin K, Lai KW, Mokhtar MI, Azizan MM, Hizaddin HF, et al.
    PeerJ Comput Sci, 2023;9:e1306.
    PMID: 37346549 DOI: 10.7717/peerj-cs.1306
    BACKGROUND: The environment has been significantly impacted by rapid urbanization, leading to a need for changes in climate change and pollution indicators. The 4IR offers a potential solution to efficiently manage these impacts. Smart city ecosystems can provide well-designed, sustainable, and safe cities that enable holistic climate change and global warming solutions through various community-centred initiatives. These include smart planning techniques, smart environment monitoring, and smart governance. An air quality intelligence platform, which operates as a complete measurement site for monitoring and governing air quality, has shown promising results in providing actionable insights. This article aims to highlight the potential of machine learning models in predicting air quality, providing data-driven strategic and sustainable solutions for smart cities.

    METHODS: This study proposed an end-to-end air quality predictive model for smart city applications, utilizing four machine learning techniques and two deep learning techniques. These include Ada Boost, SVR, RF, KNN, MLP regressor and LSTM. The study was conducted in four different urban cities in Selangor, Malaysia, including Petaling Jaya, Banting, Klang, and Shah Alam. The model considered the air quality data of various pollution markers such as PM2.5, PM10, O3, and CO. Additionally, meteorological data including wind speed and wind direction were also considered, and their interactions with the pollutant markers were quantified. The study aimed to determine the correlation variance of the dependent variable in predicting air pollution and proposed a feature optimization process to reduce dimensionality and remove irrelevant features to enhance the prediction of PM2.5, improving the existing LSTM model. The study estimates the concentration of pollutants in the air based on training and highlights the contribution of feature optimization in air quality predictions through feature dimension reductions.

    RESULTS: In this section, the results of predicting the concentration of pollutants (PM2.5, PM10, O3, and CO) in the air are presented in R2 and RMSE. In predicting the PM10 and PM2.5concentration, LSTM performed the best overall high R2values in the four study areas with the R2 values of 0.998, 0.995, 0.918, and 0.993 in Banting, Petaling, Klang and Shah Alam stations, respectively. The study indicated that among the studied pollution markers, PM2.5,PM10, NO2, wind speed and humidity are the most important elements to monitor. By reducing the number of features used in the model the proposed feature optimization process can make the model more interpretable and provide insights into the most critical factor affecting air quality. Findings from this study can aid policymakers in understanding the underlying causes of air pollution and develop more effective smart strategies for reducing pollution levels.

  2. Ahmad MI, Ab Rahim MH, Nordin R, Mohamed F, Abu-Samah A, Abdullah NF
    Sensors (Basel), 2021 Nov 17;21(22).
    PMID: 34833705 DOI: 10.3390/s21227629
    As nuclear technology evolves, and continues to be used in various fields since its discovery less than a century ago, radiation safety has become a major concern to humans and the environment. Radiation monitoring plays a significant role in preventive radiological nuclear detection in nuclear facilities, hospitals, or in any activities associated with radioactive materials by acting as a tool to measure the risk of being exposed to radiation while reaping its benefit. Apart from in occupational settings, radiation monitoring is required in emergency responses to radiation incidents as well as outdoor radiation zones. Several radiation sensors have been developed, ranging from as simple as a Geiger-Muller counter to bulkier radiation systems such as the High Purity Germanium detector, with different functionality for use in different settings, but the inability to provide real-time data makes radiation monitoring activities less effective. The deployment of manned vehicles equipped with these radiation sensors reduces the scope of radiation monitoring operations significantly, but the safety of radiation monitoring operators is still compromised. Recently, the Internet of Things (IoT) technology has been introduced to the world and offered solutions to these limitations. This review elucidates a systematic understanding of the fundamental usage of the Internet of Drones for radiation monitoring purposes. The extension of essential functional blocks in IoT can be expanded across radiation monitoring industries, presenting several emerging research opportunities and challenges. This article offers a comprehensive review of the evolutionary application of IoT technology in nuclear and radiation monitoring. Finally, the security of the nuclear industry is discussed.
  3. Saad HKM, Taib WRW, Ismail I, Johan MF, Al-Wajeeh AS, Al-Jamal HAN
    Exp Ther Med, 2021 Dec;22(6):1402.
    PMID: 34675995 DOI: 10.3892/etm.2021.10838
    Iron homeostasis is regulated by hepcidin (HEPC) that controls the dietary iron absorption and iron recycling. HEPC deficiency contributes to iron overload in β-thalassemia patients. The present study aimed to investigate the correlation between HEPC concentration and serum iron status among hemoglobin E (HbE)/β-thalassemia patients and their parents (HbE trait and β-thalassemia trait) compared with healthy controls. This study is a comparative cross-sectional study in which iron profile and HEPC level were examined in 65 HbE/β-thalassemia patients (pretransfusion) and 65 parents at the Hospital Sultanah Nur Zahirah and in 130 students as healthy controls from Univesiti Sultan Zainal Abidin, Terengganu, Malaysia. Furthermore, six samples from each group (HbE/β-thalassemia patients, parents and healthy controls) were randomly selected for gene expression analysis of HEPC and ferroportin1 (FPN1) using reverse transcription quantitative PCR. The results demonstrated that serum HEPC level were significantly decreased in HbE/β-thalassemia patients and their parents (P<0.001) compared with healthy controls. In addition, the gene expression analysis showed a dramatically downregulated HEPC in HbE/β-thalassemia patients and their parents (P=0.001) compared with healthy controls. However, there was a marked upregulation of FPN1 in HbE/β-thalassemia patients and their parents (P=0.001) compared with healthy controls. Iron profiling results revealed a significantly increased serum ferritin in HbE/β-thalassemia patients and their parents compared with healthy controls (P<0.001). In summary, the present study demonstrated that HEPC expression level and serum level were significantly decreased in HbE/β-thalassemia patients and their parents, which was combined with a marked increased FPN1 expression level and serum ferritin level compared with healthy volunteers. These findings supported the hypothesis that downregulated HEPC could lose its function as a negative regulator of FPN1, resulting in iron overload in HbE/β-thalassemia patients. Subsequently, assessing HEPC and FPN1 gene expression may be a useful tool to determine the risk of iron toxicity in patients with HbE/β-thalassemia and their parents, and could therefore be considered as a therapeutic target in the management of iron burden in these patients.
  4. Said N, Lau WJ, Ho YC, Lim SK, Zainol Abidin MN, Ismail AF
    Membranes (Basel), 2021 Oct 07;11(10).
    PMID: 34677533 DOI: 10.3390/membranes11100767
    Dialyzers have been commercially used for hemodialysis application since the 1950s, but progress in improving their efficiencies has never stopped over the decades. This article aims to provide an up-to-date review on the commercial developments and recent laboratory research of dialyzers for hemodialysis application and to discuss the technical aspects of dialyzer development, including hollow fiber membrane materials, dialyzer design, sterilization processes and flow simulation. The technical challenges of dialyzers are also highlighted in this review, which discusses the research areas that need to be prioritized to further improve the properties of dialyzers, such as flux, biocompatibility, flow distribution and urea clearance rate. We hope this review article can provide insights to researchers in developing/designing an ideal dialyzer that can bring the best hemodialysis treatment outcomes to kidney disease patients.
  5. Xueyun Z, Al Mamun A, Masukujjaman M, Rahman MK, Gao J, Yang Q
    Sci Rep, 2023 Sep 18;13(1):15438.
    PMID: 37723179 DOI: 10.1038/s41598-023-42591-3
    The phenomenon of "quiet quitting" has gained significant attention globally through various platforms, raising concerns about the impact of workplace stress on individuals' personal lives and sparking social movements and investigations. As the number of Generation Z individuals is projected to surpass millennials by 2050, understanding and addressing the quiet quitting behaviour of this generation becomes crucial, considering their negative experiences during the COVID-19 pandemic and their preference for a work-life balance, which has led to a rejection of intense competition and a desire for a more relaxed lifestyle. Thus, this study investigated the factors (work conditions, job security, perceived career development opportunities, affective organizational commitment, and perceived organizational support on job burnout and employee well-being) determining the quiet quitting intention among Chinese Gen Z employees. It used an online survey to obtain cross-sectional data from 683 respondents, which were then tested using partial least squares structural equation modelling. The results showed that work conditions, job security, perceived career progression opportunities, affective organizational commitment, and perceived organizational support had a significant positive effect on employee well-being and that job burnout had a major negative effect. Furthermore, employee well-being had a significant negative impact on China's Gen Z employees' quit quiting decision and job burnout had a significant positive influence on China's Gen Z employees' quit quiting decision. The findings provide valuable insights for organizations and practitioners, enabling them to address these factors and effectively reduce quiet quitting intentions. Moreover, this study aligns with the Social Exchange Theory (SET), which explains how the interactions between employees and their organizations influence expectations and outcomes. By considering the SET framework, organizations can understand the motivations behind employees' behaviours and make informed decisions to foster a positive work environment and enhance employee well-being.
  6. Shamsir MS, Krauss SE, Ismail IA, Ab Jalil H, Johar MA, Abdul Rahman I
    High Educ Policy, 2022;35(2):439-478.
    PMID: 34594092 DOI: 10.1057/s41307-020-00221-x
    Managing education and research during pandemics has increased in importance since the onset of epidemics such as avian flu, SARS and now CoViD-19. Successful management in times of crisis ensures business continuity and institutional survival, making preparedness preceding an impending pandemic essential. Institutions of higher education (IHEs) must maintain balance between academic continuity and preventing morbidity during a pandemic crisis. To date, however, no general pandemic preparedness frameworks exist for IHEs. The aim of this paper is to report on the development of a Haddon matrix framework for IHE pandemic preparedness based on a scoping literature review of past IHE responses including pre-, during and post-pandemic phases. First, a review of previous global responses by IHEs during past pandemics was carried out. The review findings were then collated into a new IHE-centric Haddon matrix for pandemic preparedness. The content of the matrix is then illustrated through the documented responses of Malaysian universities during the early stages of the COVID-19 pandemic. The resulting IHE Haddon matrix can be used by universities as a general guide to identify preparedness gaps and intervention opportunities for business continuity during pandemics.
  7. Ayipo YO, Bakare AA, Badeggi UM, Jimoh AA, Lawal A, Mordi MN
    Curr Res Chem Biol, 2022;2:100021.
    PMID: 35815068 DOI: 10.1016/j.crchbi.2022.100021
    Viral diseases are prominent among the widely spread infections threatening human well-being. Real-life clinical successes of the few available therapeutics are challenged by pathogenic resistance and suboptimal delivery to target sites. Nanotechnology has aided the design of functionalised and non-functionalised Au and Ag nanobiomaterials through physical, chemical and biological (green synthesis) methods with improved antiviral efficacy and delivery. In this review, innovative designs as well as interesting antiviral activities of the nanotechnology-inclined biomaterials of Au and Ag, reported in the last 5 years were critically overviewed against several viral diseases affecting man. These include influenza, respiratory syncytial, adenovirus, severe acute respiratory syndromes (SARS), rotavirus, norovirus, measles, chikungunya, HIV, herpes simplex virus, dengue, polio, enterovirus and rift valley fever virus. Notably identified among the nanotechnologically designed promising antiviral agents include AuNP-M2e peptide vaccine, AgNP of cinnamon bark extract and AgNP of oseltamivir for influenza, PVP coated AgNP for RSV, PVP-AgNPs for SARS-CoV-2, AuNRs of a peptide pregnancy-induce d hypertension and AuNP nanocarriers of antigen for MERS-CoV and SARS-CoV respectively. Others are AgNPs of collagen and Bacillus subtilis for rotavirus, AgNPs labelled Ag30-SiO 2 for murine norovirus in water, AuNPs of Allium sativum and AgNPs of ribavirin for measles, AgNPs of Citrus limetta and Andrographis Paniculata for Chikungunya, AuNPs of efavirenz and stavudine, and AgNPs-curcumin for HIV, NPAuG3-S8 for HSV, AgNPs of Moringa oleifera and Bruguiera cylindrica for dengue while AgNPs of polyethyleneimine and siRNA analogues displayed potency against enterovirus. The highlighted candidates are recommended for further translational studies towards antiviral therapeutic designs.
  8. Syed Yaacob SFF, Kamboh MA, Wan Ibrahim WA, Mohamad S
    R Soc Open Sci, 2018 Jul;5(7):171311.
    PMID: 30109037 DOI: 10.1098/rsos.171311
    A magnetic solid-phase extraction (MSPE) procedure on the newly synthesized magnetic β-cyclodextrin functionalized with toluene diisocyanate (TDI) as a linker and further modified with bio-polymeric spores of sporopollenin (MSp-TDI-βCD), was developed for the extraction of nonsteroidal anti-inflammatory drugs (NSAIDs), namely, indoprofen (INP), ketoprofen (KTP), ibuprofen (IBP) and fenoprofen (FNP) from water samples prior to their HPLC-DAD determination. The newly synthesized MSp-TDI-βCD was comprehensibly characterized using FT-IR, XRD, SEM-EDX, BET and VSM analyses. The separation of selected NSAIDs on MSp-TDI-βCD from aqueous solution was simply achieved by applying an external magnetic field via a permanent magnet. The MSPE parameters affecting extraction performance, i.e. sorbent dosage, sample volume, extraction and desorption time, type of organic eluent and volume and solution pH were investigated and optimized. The proposed method showed linear range between 0.5 and 500 ng ml-1, low limit of detection at S/N = 3 (0.16-0.37 ng ml-1) and limit of quantification at S/N = 10 (0.53-1.22 ng ml-1). The inter-day (n = 15) and intra-day (n = 5) precision for the proposed methods given by relative standard deviation (RSD%) was in the range of 2.5-4.0 and 2.1-5.5, respectively. The extraction recoveries of NSAIDs from environmental samples (tap, drinking and river water) ranged from 92.5% to 123.6%, with satisfactory precision (RSD% less than 12.4%).
  9. Malik H, Khan HW, Hassan Shah MU, Ahmad MI, Khan I, Al-Kahtani AA, et al.
    Chemosphere, 2023 Jan;311(Pt 2):136901.
    PMID: 36288769 DOI: 10.1016/j.chemosphere.2022.136901
    Ionic liquids (ILs) have been demonstrated as promising alternatives to conventional entrainers in separation of azeotropic mixtures mostly investigating phase equilibrium and process design scenarios. However, proper selection of ILs for a specific task always remains challenging. Hence a simulation tool, i.e. conductor like screening model for real solvents (COSMO-RS) was applied to address this challenge. Furthermore, screened ILs were simulated as entrainers for ethanol water separation by extractive distillation. The current study also aims to demonstrate a systematic approach to retrofit existing processes, by employing ILs as green entrainers. Screening of twenty-five (25) ILs was carried out using COSMO-RS to select suitable ILs as green entrainers based on activity coefficient, capacity and selectivity. Results illustrated that tetramethylammonium chloride ([TMAm][Cl]) due to its strong hydrogen bonding ability was found to be the best ILs entrainer. Moreover, in order to reduce the operating costs without compromising desired product purity (ethanol purity ≥99.5% in top product), the selected ILs (8 kg/h) in a mixture with ethylene glycol (72 kg/h) were simulated using Aspen plus v.11. The simulation results revealed that by combining tetramethylammonium chloride (2 kg/h) with ethylene glycol (78 kg/h) reduced 7.26 tons of CO2 emissions/year through heat integration by saving 1.49*108 kJ/year energy besides minimizing operating costs. In conclusion, the systematic selection of ILs as green entrainers in combination with ethylene glycol and then the appropriate simulation of the whole system will ultimately reduce the cost of the separation process and reduce the emission of greenhouse gases as well utilization of toxic conventional entrainers.
  10. Zahir SADM, Jamlos MF, Omar AF, Jamlos MA, Mamat R, Muncan J, et al.
    PMID: 37666099 DOI: 10.1016/j.saa.2023.123273
    Experiments demonstrated that visible and near-infrared (Vis-NIR) spectroscopy is a highly reliable tool for determining the nutritional status of plants. Although numerous studies on various kinds of plants have been conducted, there are only a few summaries of the research findings regarding the absorbance bands in the visible and near-infrared region and how they relate to the nutritional status of plants. This article will discuss the application of Vis-NIR spectroscopy for monitoring the nutrient conditions of plants, with a particular emphasis on three major components required by plants, namely nitrogen (N), phosphorus (P), and potassium (K), or NPK. Each section discussed different topics, for instance, the essential nutrients needed by plants, the application of Vis-NIR spectroscopy in nutrient status analysis, chemometrics tools, and absorbance bands related to the nutrient status, respectively. Deduction made concluded that factors affecting the plant's structure are contributed by several circumstances like the age of leaves, concentration of pigments, and water content. These factors are intertwined, strongly correlated, and can be observed in the visible and near-infrared regions. While the visible region is commonly utilised for nutritional analysis in plants, the literature review performed in this paper shows that the near-infrared region as well contains valuable information about the plant's nutritional status. A few wavelengths related to the direct estimation of nutrients in this review explained that information on nutrients can be linked with chlorophyll and water absorption bands such that N and P are the components of chlorophyll and protein; on the other hand, K exists in the form of cationic carbohydrates which are sensitive to water region.
  11. Basir A, Muhamad N, Sulong AB, Amin MBM, Jamadon NH, Radzuan NAM
    Materials (Basel), 2023 Sep 24;16(19).
    PMID: 37834512 DOI: 10.3390/ma16196375
    The micro-scale joining of two different materials using two-component micro-powder injection molding (2C-µPIM) is an intriguing technique. The formation of defects in bi-materials at different processing stages makes this technique challenging. This study presents the fabrication of defect-free bi-material micro-parts containing hydroxyapatite (HA) and 3 mol% yttria-stabilized zirconia (3YSZ) via 2C-µPIM. Critical powder volume concentrations (CPVCs) of 61.7 vol% and 47.1 vol% were obtained for the HA and 3YSZ powders, respectively. Based on the CPVCs, the optimal loadings for the HA and 3YSZ powders were selected as 60 vol% and 45 vol%, respectively. The HA and 3YSZ feedstocks were prepared by separately mixing the optimal powder contents with low-density polyethylene (LDPE) and palm stearin binders. The feedstocks displayed pseudoplastic behavior, and the lowest ranges of viscosity for the HA and 3YSZ at a temperature of 180 °C were 157.1-1392.5 Pa·s and 726.2-985.5 Pa·s, respectively. The feedstocks were injected to produce green HA/3YSZ micro-sized components. It was found that a solvent debinding temperature of 70 °C removed 60.6% of the palm stearin binder from the sample. In the thermal debinding stage, the open channels that formed in the bi-material sample's solvent debound at 70 °C and contributed to the removal of 93 to 95% of the binder system. When the debound bi-materials were sintered at 1300 °C, the highest relative density of 96.3% was obtained. The sintering operation revealed a linear shrinkage between 13 and 17% in the sintered HA/3YSZ micro-parts.
  12. Naomi R, Teoh SH, Embong H, Balan SS, Othman F, Mamat-Hamidi K, et al.
    Foods, 2023 Sep 26;12(19).
    PMID: 37835227 DOI: 10.3390/foods12193575
    Maternal obesity, characterized by an elevated body mass index (BMI) during pregnancy, is known to have adverse effects on the offspring. However, a recent study suggests that Elateriospermum tapos (E. tapos) yogurt may hold potential in mitigating excessive weight retention post-pregnancy. Thus, this study aims to employ network pharmacology to explore the pharmacological effects of the bioactive compounds present in E. tapos yogurt against maternal obesity. Initially, a screening process is conducted to identify the bioactive compounds in E. tapos yogurt, followed by the prediction of potential gene targets for these compounds using Swiss Target Prediction and the SuperPred databases. Maternal obesity-associated genes are sourced from the OMIM, DisGeNet, and GeneCards databases. The interaction between the identified compounds and maternal obesity genes is established via protein-protein interaction analysis, gene ontology examination, and KEGG pathway analysis. To validate the results, molecular docking studies are conducted using AutoDock Tools software. The findings reveal that out of the 64 compounds analyzed, three meet the screening criteria, resulting in a total of 380 potential gene targets. Among these targets, 240 are shared with maternal obesity-related genes. Further analysis demonstrates the favorable affinity of these active compounds with key targets, linking them to biological processes involving protein phosphorylation, inflammation, as well as the pathways related to lipid metabolism, atherosclerosis, and the other signaling pathways. In conclusion, this study provides valuable insights into the potential pharmacological effects of the bioactive compounds found in E. tapos yogurt against maternal obesity. These findings open avenues for further exploration and potential therapeutic interventions targeting maternal obesity.
  13. Sa'don SNH, Jamaluddin MH, Kamarudin MR, Ahmad F, Yamada Y, Kamardin K, et al.
    Sensors (Basel), 2019 Nov 06;19(22).
    PMID: 31698830 DOI: 10.3390/s19224835
    The incoming 5G technology requires antennas with a greater capacity, wider wireless spectrum utilisation, high gain, and steer-ability. This is due to the cramped spectrum utilisation in the previous generation. As a matter of fact, conventional antennas are unable to serve the new frequency due to the limitations in fabrication and installation mainly for smaller sizes. The use of graphene material promises antennas with smaller sizes and thinner dimensions, yet capable of emitting higher frequencies. Hence, graphene antennas were studied at a frequency of 15 GHz in both single and array elements. The high-frequency antenna contributed to a large bandwidth and was excited by coplanar waveguide for easy fabrication on one surface via screen printing. The defected ground structure was applied in an array element to improve the radiation and increase the gain. The results showed that the printed, single element graphene antenna produced an impedance bandwidth, gain, and efficiency of 48.64%, 2.87 dBi, and 67.44%, respectively. Meanwhile, the array element produced slightly better efficiency (72.98%), approximately the same impedance bandwidth as the single element (48.98%), but higher gain (8.41 dBi). Moreover, it provided a beam width of 21.2° with scanning beam capability from 0° up to 39.05°. Thus, it was proved that graphene materials can be applied in 5G.
  14. Nawawi FA, Wan Md Adnan WAH, Ismail M, Jalaludin MY, Majid HA
    Kidney Int Rep, 2023 Oct;8(10):1965-1977.
    PMID: 37850001 DOI: 10.1016/j.ekir.2023.07.028
    INTRODUCTION: The health and wellbeing of adolescents are often neglected, including the knowledge of chronic kidney disease (CKD), especially in its early stages.

    METHODS: A total of 607 adolescents were recruited from the Malaysian Health and Adolescents Longitudinal Research Team (MyHeART) study, a prospective cohort study conducted from March 2012 to May 2016 that explored the noncommunicable diseases (NCDs) risk factors among 13 to 17 years old students in 3 states of Peninsular Malaysia. Students who participated in all 3 data collection periods in 2012, 2014, and 2016 with kidney function assessment across all 3-time points were included in the current study. The students' estimated glomerular filtration rate (eGFR) was calculated from isotope-dilution mass spectrometry-traceable Schwartz's equation and categorized based on Kidney Disease: Improving Global Outcomes (KDIGO) classification. Changes in kidney function were examined, and the longitudinal relationship between eGFR and multiple NCD risk factors was analyzed using the generalized estimating equation (GEE).

    RESULTS: The prevalence of decreased eGFR (60-89 ml/min per 1.73 m2) among the students increased from 6.1% (2012) to 30.0% (2014) and 40.2% (2016). Based on the GEE, the student's eGFR decreased over time, with a steeper decline during early to midadolescence. Males and rural students had lower eGFR compared to their counterparts. Students who are morbidly obese had lower eGFR than those with normal body mass index (BMI). Protein consumption also has a potential moderating effect on eGFR in adolescents.

    CONCLUSION: Kidney function changes can be detected as early as adolescence and are likely attributable to multiple NCD risk factors. Therefore, more comprehensive prevention efforts from various stakeholders are needed to identify health issues like CKD.

  15. Ishak NF, Wan Azhar WMA, Ahmad S, Khairuddin AU, Laboh R
    Plant Dis, 2023 Oct 19.
    PMID: 37858968 DOI: 10.1094/PDIS-06-23-1076-PDN
    In Malaysia, bell pepper (Capsicum annuum var. grossum), also known as sweet pepper or paprika, is one of the highly imported vegetable crops. In 2021 alone, Malaysia imported nearly 74 thousand metric tons of its chilies, including bell peppers, from other countries (DOSM, 2022). Often, farmers grow the bell peppers in moderate to cool conditions within highland regions for local commercial purposes. In June 2022, the Malaysian Agricultural Research and Development Institute (MARDI) in Serdang, Selangor, conducted a research study to grow lowland bell peppers under a glasshouse rain protection system. A disease inspection carried out found fruit rot on approximately 30% of mature bell pepper fruits in the greenhouse. Symptoms appeared as firm and sunken black lesions covered with white to light pink spore masses on the outer surface, which eventually fell off. Infected fruit parts were disinfected with 10% hypochlorite (NaOCl) for 2 min, followed by double washing with sterile distilled water, air-dried, and placed onto potato dextrose agar (PDA). After 3 days of incubation, the fungal colonies that grew from the symptomatic tissue pieces were transferred onto new PDA to obtain pure cultures. The pure fungal colony appeared dense, whitish aerial mycelium that slowly became cream to pinkish-orange after 7 days of incubation at room temperature (25±2 °C). To examine the morphology features, the pure cultures were subbed onto carnation leaf agar (CLA) and incubated at 25±2°C for 14 days. Macroconidia were abundant, slightly curved with tapered apical cells, 3- to 5-septate, and ranged between 21.8 and 34.0 x 3.0 and 5.1 μm. Microconidia were single-celled, often 1-septate, and ranged between 10.0 and 12.6 x 2.1 and 3.4 μm. Chlamydospores were globose and in chains. The fungus was identified as Fusarium sp. according to Fusarium key by Leslie and Summerell (2006). PCR amplification and DNA sequencing were performed using primers EF1F/EF2R and ITS1/ITS4 (O'Donnell et al., 1998; White et al., 1990) to amplify the partial elongation factor 1-alpha (TEF1-α) gene and internal transcribed spacer region (ITS), respectively. The TEF1-α and ITS sequences of this isolate were deposited in GenBank as OQ672911 and OR349657. BLAST analysis with TEF1-α gene sequences revealed 99.74% and 99.33% sequence identity with F. pernambucanum (accession no. ON330424) and Fusarium isolate NRRL 25134 (accession no. JF740755), respectively; both belonged to the Fusarium incarnatum-equiseti species complex (FIESC). BLAST search of the TEF1-α sequence in the database of the International Mycological Association (www.mycobank.org) showed 99.18% identity with FIESC (NRRL 36548). The ITS sequences were 100% identical to those of F. incarnatum (MT563420, MT563419, and MT563418). Pathogenicity test was conducted on three unwounded and three wounded mature red bell pepper fruits (SP299 Red Masta variety). Two healthy bell peppers were used as controls for each treatment. Prior to inoculation, the fruits were surface-sterilized by dipping in 70% ethanol and rinsed twice with sterile distilled water. Unwounded fruits were inoculated with fungal mycelium disks (5 mm diameter), whereas control fruits were inoculated with sterile PDA agar disks. For wound method, 6 µl of spore suspension (1x106 spores/ml) was obtained from 7-day-old cultures and injected (1 mm depth) into the fruit wall using a sterile syringe needle. Control fruits were inoculated with sterile distilled water only. Each fruit was inoculated with the inoculum at three distinct spots and kept in a humid chamber at a temperature of 25±2 °C. The pathogenicity test was done twice. Five days post-inoculation, the control fruits showed no symptoms, whereas all inoculated wounded and non-wounded fruits developed necrotic lesions with white mycelium growing on the inoculation points. The pathogen was successfully re-isolated from the infected fruits and morphologically identified as FIESC, fulfilling Kochs postulates. It has been reported previously that the members of FIESC are responsible for the fruit rot of bell peppers under greenhouse conditions (Ramdial et al., 2016). To the best of our knowledge, this is the first report of FIESC causing fruit rot on greenhouse bell peppers in Malaysia. This fruit rot disease may impose significant constraints on bell pepper production in Malaysia; hence, effective strategies to control the pathogen and prevent disease dispersal should be implemented.
  16. Abdul Samad FD, Pereira XV, Chong SK, Abdul Latif MHB
    Front Psychiatry, 2023;14:1218715.
    PMID: 37840803 DOI: 10.3389/fpsyt.2023.1218715
    Interpersonal psychotherapy (IPT) is a highly regarded evidence-based psychotherapy that aims to alleviate the suffering of clients and improve their interpersonal functioning. Research has demonstrated the effectiveness of IPT in depressive, bipolar and eating disorders. IPT also focuses on grief and loss as a problem area to help clients address and process their grief symptoms, leading them to reach a phase of finding meaning. However, traumatic grief which is characterized by someone who has both symptoms of trauma and grief can further complicate treatment. As for Posttraumatic Stress Disorder (PTSD), IPT can be a choice of treatment by addressing perceived isolation and emotional dysregulation through mobilizing adequate social support. This case study highlights the efficacy of IPT in treating complicated grief with traumatic experiences caused by the loss of a loved one during the COVID-19 pandemic, without undergoing exposure-based therapy. The treatment course consisted of 12 sessions scheduled twice weekly, and the client received antidepressant medication augmented with antipsychotic medication. After undergoing IPT, the client experienced an improvement in symptoms, gradual recovery of functional disability, and more meaningful interpersonal relationships. The case study presented provides evidence to suggest that IPT is a promising treatment approach for individuals struggling with trauma related to grief.
  17. Rehman A, Abbas S, Khan MA, Ghazal TM, Adnan KM, Mosavi A
    Comput Biol Med, 2022 Nov;150:106019.
    PMID: 36162198 DOI: 10.1016/j.compbiomed.2022.106019
    In recent years, the global Internet of Medical Things (IoMT) industry has evolved at a tremendous speed. Security and privacy are key concerns on the IoMT, owing to the huge scale and deployment of IoMT networks. Machine learning (ML) and blockchain (BC) technologies have significantly enhanced the capabilities and facilities of healthcare 5.0, spawning a new area known as "Smart Healthcare." By identifying concerns early, a smart healthcare system can help avoid long-term damage. This will enhance the quality of life for patients while reducing their stress and healthcare costs. The IoMT enables a range of functionalities in the field of information technology, one of which is smart and interactive health care. However, combining medical data into a single storage location to train a powerful machine learning model raises concerns about privacy, ownership, and compliance with greater concentration. Federated learning (FL) overcomes the preceding difficulties by utilizing a centralized aggregate server to disseminate a global learning model. Simultaneously, the local participant keeps control of patient information, assuring data confidentiality and security. This article conducts a comprehensive analysis of the findings on blockchain technology entangled with federated learning in healthcare. 5.0. The purpose of this study is to construct a secure health monitoring system in healthcare 5.0 by utilizing a blockchain technology and Intrusion Detection System (IDS) to detect any malicious activity in a healthcare network and enables physicians to monitor patients through medical sensors and take necessary measures periodically by predicting diseases. The proposed system demonstrates that the approach is optimized effectively for healthcare monitoring. In contrast, the proposed healthcare 5.0 system entangled with FL Approach achieves 93.22% accuracy for disease prediction, and the proposed RTS-DELM-based secure healthcare 5.0 system achieves 96.18% accuracy for the estimation of intrusion detection.
  18. Alrosan M, Tan TC, Koh WY, Easa AM, Gammoh S, Alu'datt MH
    Crit Rev Food Sci Nutr, 2023;63(25):7677-7691.
    PMID: 35266840 DOI: 10.1080/10408398.2022.2049200
    Demands for high nutritional value-added food products and plant-based proteins have increased over the last decade, in line with the growth of the human population and consumer health awareness. The quality of the plant-based proteins depends on their digestibility, amino acid content, and residues of non-nutritive compounds, such as phenolic compounds, anti-nutritional compounds, antioxidants, and saponins. The presence of these non-nutritive compounds could have detrimental effects on the quality of the proteins. One of the solutions to address these shortcomings of plant-based proteins is fermentation, whereby enzymes that present naturally in microorganisms used during fermentation are responsible for the cleavage of the bonds between proteins and non-nutritive compounds. This mechanism has pronounced effects on the non-nutritive compounds, resulting in the enhancement of protein digestibility and functional properties of plant-based proteins. We assert that the types of plant-based proteins and microorganisms used during fermentation must be carefully addressed to truly enhance the quality, functional properties, and health functionalities of plant-based proteins.Supplemental data for this article is available online at here. show.
  19. Qazi A, Raj RG, Tahir M, Cambria E, Syed KB
    ScientificWorldJournal, 2014;2014:879323.
    PMID: 25054188 DOI: 10.1155/2014/879323
    Appropriate identification and classification of online reviews to satisfy the needs of current and potential users pose a critical challenge for the business environment. This paper focuses on a specific kind of reviews: the suggestive type. Suggestions have a significant influence on both consumers' choices and designers' understanding and, hence, they are key for tasks such as brand positioning and social media marketing. The proposed approach consists of three main steps: (1) classify comparative and suggestive sentences; (2) categorize suggestive sentences into different types, either explicit or implicit locutions; (3) perform sentiment analysis on the classified reviews. A range of supervised machine learning approaches and feature sets are evaluated to tackle the problem of suggestive opinion mining. Experimental results for all three tasks are obtained on a dataset of mobile phone reviews and demonstrate that extending a bag-of-words representation with suggestive and comparative patterns is ideal for distinguishing suggestive sentences. In particular, it is observed that classifying suggestive sentences into implicit and explicit locutions works best when using a mixed sequential rule feature representation. Sentiment analysis achieves maximum performance when employing additional preprocessing in the form of negation handling and target masking, combined with sentiment lexicons.
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