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Fulltext Real-time PCR evaluation of seven DNA extraction methods for the purpose of GMO analysis

Jasbeer, K., Son, R., Mohamad Ghazali, F., Cheah, Y.K.

International Food Research Journal, 2009;16(3):329-341.
MyJurnal

Successful DNA amplification is vital for the detection of specific DNA targets in feeds, and this in return depends on the ability of DNA extraction methods to produce good quality DNA. In this study, seven methods were compared for DNA extraction from feeds using quantitative polymerase chain reaction (PCR) of single copy maize (Zea mays) endogenous hmg (high mobility group) gene. Relative levels of hmg were used to evaluate the DNA quality. Spectrophotometer determination of DNA was also carried out to assess DNA yield and DNA purity, while electrophoretic analysis of genomic DNA extracts was carried out to investigate DNA integrity. The findings illustrate that the DNA extraction methods have a significant effect on DNA quality. Statistically, the Epicentre method extracted the highest DNA yield while the Wizard method had the lowest DNA yield with high DNA purity and integrity. However, the Wizard method recovered the most amplifiable DNA per reaction, indicating that template quality and integrity had greater influence over hmg amplification than DNA yield.

Matched MeSH terms: DNA
Fulltext Halal market surveillance of soft and hard gel capsules in pharmaceutical products using PCR and southern-hybridization on the biochip analysis

Sahilah, A.M., Mohd Fadly, L., Norrakiah, A.S., Aminah, A., Wan Aida, W.M., Ma'aruf, A.G., et al.

International Food Research Journal, 2012;19(1):371-375.
MyJurnal

The study was conducted to detect the porcine DNA in pharmaceutical products in local market using polymerase chain reaction (PCR) and southern-hybridization on the biochip. A total of 113 (n=113) of hard (82 samples) and soft gel (31 samples) capsules from pharmaceutical products were purchased and tested for the presence of porcine DNA for Halal authentication. All capsules were gelatin-based purchased from local over the counter (OTC) markets. Of all samples tested, 37.2% (42/113) contained porcine DNA. While, none porcine DNA band was detected for 62.8% (71/113) of capsules tested. All samples which were positive toward porcine DNA were imported pharmaceutical products with none Halal logo. Results in the presence study demonstrated that the PCR techniques and southern-hybridization on the biochip is suitable tool for monitoring the Haram component in highly processed product of soft and hard capsule.

Matched MeSH terms: DNA
Fulltext Development of Tat-Conjugated Dendrimer for Transdermal DNA Vaccine Delivery

Bahadoran A, Moeini H, Bejo MH, Hussein MZ, Omar AR

J Pharm Pharm Sci, 2016 Jul-Sep;19(3):325-338.
PMID: 27806247 DOI: 10.18433/J3G31Q

PURPOSE: In order to enhance cellular uptake and to facilitate transdermal delivery of DNA vaccine, polyamidoamine (PAMAM) dendrimers conjugated with HIV transactivator of transcription (TAT) was developed.

METHODS: First, the plasmid DNA (pIRES-H5/GFP) nanoparticle was formulated using PAMAM dendrimer and TAT peptide and then characterized for surface charge, particle size, DNA encapsulation and protection of the pIRES-H5/GFP DNA plasmid to enzymatic digestion. Subsequently, the potency of the TAT-conjugated dendrimer for gene delivery was evaluated through in vitro transfection into Vero cells followed by gene expression analysis including western blotting, fluorescent microscopy and PCR. The effect of the TAT peptide on cellular uptake of DNA vaccine was studied by qRT-PCR and flow cytometry. Finally, the ability of TAT-conjugated PAMAM dendrimer for transdermal delivery of the DNA plasmid was assessed through artificial membranes followed by qRT-PCR and flow cytometry.

RESULTS: TAT-conjugated PAMAM dendrimer showed the ability to form a compact and nanometre-sized polyplexes with the plasmid DNA, having the size range of 105 to 115 nm and a positive charge of +42 to +45 mV over the N/P ratio of 6:1(+/-). In vitro transfection analysis into Vero cells confirms the high potency of TAT-conjugated PAMAM dendrimer to enhance the cellular uptake of DNA vaccine. The permeability value assay through artificial membranes reveals that TAT-conjugated PAMAM has more capacity for transdermal delivery of the DNA compared to unmodified PAMAM dendrimer (P<0.05).

CONCLUSIONS: The findings of this study suggest that TAT-conjugated PAMAM dendrimer is a promising non-viral vector for transdermal use.This article is open to POST-PUBLICATION REVIEW. Registered readers (see "For Readers") may comment by clicking on ABSTRACT on the issue's contents page.

Matched MeSH terms: Vaccines, DNA/genetics; Vaccines, DNA/pharmacokinetics*; Vaccines, DNA/chemistry*
Fulltext Resurrection of Bronchocela burmana Blanford, 1878 for the Green Crested Lizard (Squamata, Agamidae) of southern Myanmar

Zug GR, Mulcahy DG, Vindum JV

Zookeys, 2017.
PMID: 28331413 DOI: 10.3897/zookeys.657.11600

Recent fieldwork in southern Tanintharyi revealed the presence of a small Green Crested Lizard in the wet evergreen forest. We generated mtDNA sequence data (ND2) that demonstrates that this population's nearest relative is Bronchocela rayaensis Grismer et al., 2015 of Pulau Langkawi, northwestern Peninsular Malaysia and Phuket Island. Morphologically the Burmese Bronchocela shares many features with Bronchocela rayaensis, which potentially would make this recently described Thai-Malay species a synonym of Bronchocela burmana Blanford, 1878; however, we interpret the genetic and morphological differences to reflect evolutionary divergence and recommend the recognition of both species.

Matched MeSH terms: DNA, Mitochondrial
Fulltext Molecular conservation strategies for Malaysian endemic species

Rodrigues, K. F.

Buletin Persatuan Genetik Malaysia, 2008;14(1):4-8.
MyJurnal

Molecular techniques involving the application of DNA based molecular markers for the conservation and management of endemic and endangered species have assumed significance as
genome sequencing projects have generated an extensive database which can be mined for informative genomic regions. Scientific approaches towards conservation involve several stages, which encompass determination of appropriate genomic regions for characterization, design and testing of specific molecular markers, screening of multiple populations and statistical treatment and
interpretation of data. Population data can be utilized to develop controlled breeding and relocation programs aimed at ensuring that genetic diversity within populations of endangered species is
sustained within the context of an overall conservation program. The information derived as a result of this approach can be applied to establish a scientific and legal framework for the conservation of endemic species. Species specific genomic markers can be applied to enforce the implementation of CITES within the guidelines of a national biodiversity conservation policy.

Matched MeSH terms: DNA
Fulltext Effects of ion beam irradiation on Oncidium lanceanum orchids

Zaiton Ahmad, Affrida Abu Hassan, Mohd Nazir Basiran, Nurul Aliaa Idris, Tanaka, A., Shikazono, N., et al.

Journal of Nuclear and Related Technologies, 2006;2006(1):1-7.
MyJurnal

Protocorm-like bodies (PLBs) of an orchid (Oncidium lanceanum) were irradiated using 220 MeV 12 C 5+ ions, accelerated by AVF cyclotron at JAEA, Japan in 2005. Five different doses were applied to the PLBs; 0, 1.0, 2.0, 6.0 and 12.0 Gy. Following irradiation, these PLBs were maintained in cultures for germination and multiplication. Irradiation effects on growth and seedling regeneration patterns as well as molecular characteristics of the in vitro cultures were monitored and recorded. In general, average fresh weights of the irradiated PLBs increased progressively by irradiating the explants at 1.0, 2.0 and reached the maximum at 6.0 Gy. The figure however dropped when the explants were irradiated at 12 Gy. Surprisingly, although the highest average fresh weight was recorded on PLBs irradiated at 6.0 Gy, most of these PLBs were not able to regenerate into complete shoots. On average, after 4 months of irradiation, only 21 seedlings were successfully regenerated from each gram of these PLBs. The highest shoot regeneration was recorded on cultures irradiated at 2.0 Gy in which 102 seedlings were obtained from one gram of the PLBs. Some morphological changes were seen on in vitro plantlets derived from PLBs irradiated at doses of 1.0 and 2.0 Gy. Most of the regenerated seedlings have been transferred to glasshouse for further morphological selection. Molecular analysis showed the presence of DNA polymorphisms among the seedlings from different doses of irradiation.

Matched MeSH terms: DNA
Fulltext Pengaktifan otak akibat gerakan jari bagi subjek dominan tangan kanan dan kiri

Ahmad Nazlim Yusoff, Mohd Harith Hashim, Mohd Mahadir Ayob, Iskandar Kassim

Jurnal Sains Kesihatan Malaysia, 2006;4(2):63-83.
MyJurnal

Kajian garis pangkal pengimejan resonans magnet kefungsian (fMRI) telah dijalankan ke atas 2 orang subjek lelaki sihat dominan tangan kanan dan kiri. Kajian ini menggunakan gerakan jari tangan kanan dan kiri untuk merangsang aktiviti neuron di dalam korteks serebrum. Subjek diarahkan supaya menekan jari-jari pada ibu jari secara bergilir-gilir semasa imbasan fMRI dilakukan. Paradigma 5 kitar aktif-rehat digunakan dengan setiap kitar mengandungi satu blok aktif dan satu blok rehat dengan 10 siri pengukuran untuk setiap blok. Seratus isipadu imej fMRI bagi setiap subjek dianalisis menggunakan pekej perisian MatLab dan SPM2. Model linear am (GLM) digunakan untuk menganggar secara statistik parameter yang mencirikan model rangsangan hemodinamik bagi gerakan jari. Kesimpulan mengenai pengaktifan otak yang diperhatikan dijana secara statistik berasaskan teori medan rawak (RFT) Gaussian. Keputusan menunjukkan bahawa rantau otak yang aktif akibat gerakan jari adalah pada girus presentral merangkumi kawasan motor primer. Pengaktifan otak adalah secara kontralateral terhadap gerakan jari tangan kanan dan kiri. Keamatan isyarat keadaan aktif didapati lebih tinggi secara bererti (p < 0.001) daripada keamatan isyarat keadaan rehat. Bilangan voksel yang aktif didapati lebih tinggi pada hemisfera otak yang mengawal gerakan jari bagi tangan yang tidak dominan untuk kedua-dua subjek. Keputusan ini menyokong fakta bahawa kawasan pengaktifan motor pada hemisfera otak semasa gerakan jari tangan yang tidak dominan mengalami rangsangan hemodinamik yang lebih tinggi dan kawasan pengaktifan yang lebih luas berbanding dengan kawasan pengaktifan pada hemisfera otak yang mengawal gerakan jari bagi tangan yang dominan.

Matched MeSH terms: DNA Primers
Fulltext Human variome project and launching of it’s Malaysian node; towards a new horizon of genetics in Malaysia

Atif A. B., Halim-Fikri A H, Zilfalil BA

Buletin Persatuan Genetik Malaysia, 2010;17(1):9-11.
MyJurnal

In the human genome, point variations are most common (Nachman & Crowell, 2000) and well understood. These variations, when existing in more than 1% of the population, is referred to as
Single Nucleotide Polymorphism (SNP) and can fall in the coding region of a gene, non coding region or intergenic regions.

Matched MeSH terms: DNA, Intergenic
Fulltext DNA horizontal polyacrylmide gel electrophoresis

Elsie Yee, Y. S., Zainal Zahari, AHMAD ISMAIL, YAP, C.K., TAN, S. G

Buletin Persatuan Genetik Malaysia, 2010;16(1):19-22.
MyJurnal

Electrophoresis is a crucial step for the studies of proteins, allozymes, DNAs and RNAs. Two commonly used electrophoresis systems are agarose gel and polyacrylmide gel. Agarose gel is frequently used for DNAs and RNAs studies whereas polyacrylmide gel is widely used for the studies of other macromolecules such as proteins, allozymes (isozymes), DNAs and RNAs. The banding patterns of the gels, rather than the numbers of bands appearing on the gels are important for scoring in fingerprinting, footprinting and in population genetic studies.

Matched MeSH terms: DNA
Cloning and sequencing of its region of two isolates of Phellinus spp obtained from diseased roots of setang (Azadirachta excelsa [Jack] Jacobs)

Mohd Rosli Haron, Mohd Farid Ahmad, Lee, Su See, Norwati Muhammad

Buletin Persatuan Genetik Malaysia, 2005;11(1):0-0.
MyJurnal

Two isolates of brown root disease fungi were obtained from diseased roots of sentang (Azadirachta excelsa). Morphological characters from macroscopic and microscopic studies suggested that both isolates were from the same genus namely Phellinus noxius and Phellinus sp. Cloning and sequencing of ITS region were conducted to investigate further the variation between the two species at
molecular level. PCR-amplified ITS regions were cloned in pCR2.1 and sequenced. DNA sequences sized 723bp and 710bp were obtained for Phellinus noxius and Phellinus sp respectively. Comparison between the two sequences showed 98% similarity where three nucleotide substitutions and three insertion/deletion regions were found sized 8bp, 2bp and 3bp respectively.

Matched MeSH terms: DNA
Cell Proliferation and DNA Repair Ability of Ganoderma neo-japonicum (Agaricomycetes): An Indigenous Medicinal Mushroom from Malaysia

Tan WC, Kuppusamy UR, Phan CW, Sabaratnam V

Int J Med Mushrooms, 2018;20(2):155-163.
PMID: 29773007 DOI: 10.1615/IntJMedMushrooms.2018025445

Ganoderma neo-japonicum is an annual polypore that grows on decaying bamboo in the forests of Malaysia. The indigenous Temuan tribe uses this species as a medicinal mushroom to cure fever and epilepsy and to improve body strength. The potential use of G. neo-japonicum in genoprotection and DNA repair was established using a single-cell gel electrophoresis (comet) assay. The effects of the ethanol and hot aqueous extracts from wild and cultivated basidiocarps, solid substrate-fermented (SSF) wheat grains, and mycelia via submerged culture on H2O2-damaged murine RAW264.7 macrophages were investigated. An ethanol extract from wild basidiocarps showed the most significant protective effect on murine RAW264.7 macrophages, followed by ethanol and hot water extracts of cultivated basidiocarps, and this effect was dose dependent. However, only the ethanol extracts from SSF and submerged culture showed significant protective effects compared with the control. As for DNA repair ability, only the ethanol extract from wild and cultivated basidiocarps showed significant results when compared with the negative control. The findings suggest the potential therapeutic use of G. neo-japonicum in genome protection and as a DNA repair stimulator.

Matched MeSH terms: DNA Damage/drug effects; DNA Repair/drug effects*
Fulltext Mutations inside rifampicin-resistance determining region of rpoB gene associated with rifampicin-resistance in Mycobacterium tuberculosis

Zaw MT, Emran NA, Lin Z

J Infect Public Health, 2018 04 26;11(5):605-610.
PMID: 29706316 DOI: 10.1016/j.jiph.2018.04.005

BACKGROUND: Rifampicin (RIF) plays a pivotal role in the treatment of tuberculosis due to its bactericidal effects. Because the action of RIF is on rpoB gene encoding RNA polymerase β subunit, 95% of RIF resistant mutations are present in rpoB gene. The majority of the mutations in rpoB gene are found within an 81bp RIF-resistance determining region (RRDR).
METHODOLOGY: Literatures on RIF resistant mutations published between 2010 and 2016 were thoroughly reviewed.
RESULTS: The most commonly mutated codons in RRDR of rpoB gene are 531, 526 and 516. The possibilities of absence of mutation in RRDR of rpoB gene in MDR-TB isolates in few studies was due to existence of other rare rpoB mutations outside RRDR or different mechanism of rifampicin resistance.
CONCLUSION: Molecular methods which can identify extensive mutations associated with multiple anti-tuberculous drugs are in urgent need so that the research on drug resistant mutations should be extended.

Matched MeSH terms: DNA-Directed RNA Polymerases
Fulltext Optical DNA Biosensor Based on Square-Planar Ethyl Piperidine Substituted Nickel(II) Salphen Complex for Dengue Virus Detection

Ariffin EY, Tan LL, Abd Karim NH, Yook Heng L

Sensors (Basel), 2018 Apr 12;18(4).
PMID: 29649118 DOI: 10.3390/s18041173

A sensitive and selective optical DNA biosensor was developed for dengue virus detection based on novel square-planar piperidine side chain-functionalized N,N'-bis-4-(hydroxysalicylidene)-phenylenediamine-nickel(II), which was able to intercalate via nucleobase stacking within DNA and be functionalized as an optical DNA hybridization marker. 3-Aminopropyltriethoxysilane (APTS)-modified porous silica nanospheres (PSiNs), was synthesized with a facile mini-emulsion method to act as a high capacity DNA carrier matrix. The Schiff base salphen complexes-labelled probe to target nucleic acid on the PSiNs renders a colour change of the DNA biosensor to a yellow background colour, which could be quantified via a reflectance transduction method. The reflectometric DNA biosensor demonstrated a wide linear response range to target DNA over the concentration range of 1.0 × 10-16-1.0 × 10-10 M (R² = 0.9879) with an ultralow limit of detection (LOD) at 0.2 aM. The optical DNA biosensor response was stable and maintainable at 92.8% of its initial response for up to seven days of storage duration with a response time of 90 min. The reflectance DNA biosensor obtained promising recovery values of close to 100% for the detection of spiked synthetic dengue virus serotypes 2 (DENV-2) DNA concentration in non-invasive human samples, indicating the high accuracy of the proposed DNA analytical method for early diagnosis of all potential infectious diseases or pathological genotypes.

Matched MeSH terms: DNA
Fulltext Molecular identification of a rare haemoglobin variant: Hb G coushatta in Malaysia

Alifah Nadia Abu Hassan, Ezalia Esa, Nur Aisyah Aziz, Faidatul Syazlin Abd Hamid, Zubaidah Zakaria, Siti Aisyah Lazim

Journal of Biomedical and Clinical Sciences, 2017;2(202):1-2.
MyJurnal

Thalassaemia screening programme was conducted to reduce the burden of the disease [1]. Here, we describe one unexpected discovery in a 33-year-old gentleman and also the importance of DNA analysis in detecting the globin gene mutation.

Matched MeSH terms: DNA
Fulltext Antibiotic susceptibility and genotyping by RAPD of Campylobacter jejuni isolated from retailed ready-to-eat sushi

Tan, Y.F., Haresh, K.K., Chai, L.C., Son R.

International Food Research Journal, 2009;16(1):31-38.
MyJurnal

A study to determine the antibiotic sensitivity pattern and genotyping using RAPD-PCR was performed on 50 C. jejuni isolated from sushi retailed in different supermarkets. With less than half of the isolates susceptible to the antibiotics tested, resistant to two or more antibiotics were observed in most of the isolates. The banding patterns obtained from RAPD-PCR revealed that no predominant clone exists and the bacterial population is rather diverse. Hence, the resistance of the C. jejuni to different classes of antibiotic as well as their diverse genotypes suggests that these C. jejuni isolates were generated from different sources in the contaminated supermarkets where sushi were retailed. Our data showed that C. jejuni can be an important reservoir for resistance genes and that study with comprehensive collections of samples are urgently required to establish better measures to reduce or eliminate the risk from antibiotic resistant and pathogenic bacteria originating from minimally processed ready-to-eat food.

Matched MeSH terms: Random Amplified Polymorphic DNA Technique
Fulltext Combating transnational organized crime by linking multiple large ivory seizures to the same dealer

Wasser SK, Torkelson A, Winters M, Horeaux Y, Tucker S, Otiende MY, et al.

Sci Adv, 2018 09;4(9):eaat0625.
PMID: 30255141 DOI: 10.1126/sciadv.aat0625

Rapid growth in world trade has enabled transnational criminal networks to conceal their contraband among the 1 billion containers shipped worldwide annually. Forensic methods are needed to identify the major cartels moving the contraband into transit. We combine DNA-based sample matching and geographic assignment of tusks to show that the two tusks from the same elephant are often shipped by the same trafficker in separate large consignments of ivory. The paired shipments occur close in time from the same initial place of export and have high overlap in the geographic origins of their tusks. Collectively, these paired shipments form a linked chain that reflects the sizes, interconnectedness, and places of operation of Africa's largest ivory smuggling cartels.

Matched MeSH terms: DNA
Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia

Senapin S, Dong HT, Meemetta W, Gangnonngiw W, Sangsuriya P, Vanichviriyakit R, et al.

J Fish Dis, 2019 Jan;42(1):119-127.
PMID: 30397913 DOI: 10.1111/jfd.12915

In Southeast Asia, a new disease called scale drop disease (SDD) caused by a novel Megalocytivirus (SDDV) has emerged in farmed Asian sea bass (Lates calcarifer) in Singapore, Malaysia and Indonesia. We received samples from an Eastern Thai province that also showed gross signs of SDD (loss of scales). Clinical samples of 0.2-1.1 kg L. calcarifer collected between 2016 and 2018 were examined for evidence of SDDV infection. Histopathology was similar to that in the first report of SDDV from Singapore including necrosis, inflammation and nuclear pyknosis and karyorrhexis in the multiple organs. Intracytoplasmic inclusion bodies were also observed in the muscle tissue. In a density-gradient fraction from muscle extracts, TEM revealed enveloped, hexagonal megalocytiviral-like particles (~100-180 nm). By PCR using primers derived from the Singaporean SDDV genome sequence, four different genes were amplified and sequenced from the Thai isolate revealing 98.7%-99.9% identity between the two isolates. Since viral inclusions were rarely observed, clinical signs and histopathology could not be used to easily distinguish between SDD caused by bacteria or SDDV. We therefore recommend that PCR screening be used to monitor broodstock, fry and grow-out fish to estimate the current impact of SDDV in Southeast Asia and to prevent its spread.

Matched MeSH terms: DNA Virus Infections/mortality; DNA Virus Infections/pathology; DNA Virus Infections/veterinary*
Fulltext An Electrochemical DNA Biosensor for Carcinogenicity of Anticancer Compounds Based on Competition between Methylene Blue and Oligonucleotides

Md Sani ND, Ariffin EY, Sheryn W, Shamsuddin MA, Heng LY, Latip J, et al.

Sensors (Basel), 2019 Nov 22;19(23).
PMID: 31766637 DOI: 10.3390/s19235111

A toxicity electrochemical DNA biosensor has been constructed for the detection of carcinogens using 24 base guanine DNA rich single stranded DNA, and methylene blue (MB) as the electroactive indicator. This amine terminated ssDNA was immobilized onto silica nanospheres and deposited on gold nanoparticle modified carbon-paste screen printed electrodes (SPEs). The modified SPE was initially exposed to a carcinogen, followed by immersion in methylene blue for an optimized duration. The biosensor response was measured using differential pulse voltammetry. The performance of the biosensor was identified on several anti-cancer compounds. The toxicity DNA biosensor demonstrated a linear response range to the cadmium chloride from 0.0005 ppm to 0.01 ppm (R2 = 0.928) with a limit of detection at 0.0004 ppm. The biosensor also exhibited its versatility to screen the carcinogenicity of potential anti-cancer compounds.

Matched MeSH terms: DNA/chemistry*; DNA, Single-Stranded/chemistry
Rapid detection of porcine DNA in processed food samples using a streamlined DNA extraction method combined with the SYBR Green real-time PCR assay

Tan LL, Ahmed SA, Ng SK, Citartan M, Raabe CA, Rozhdestvensky TS, et al.

Food Chem, 2020 Mar 30;309:125654.
PMID: 31678669 DOI: 10.1016/j.foodchem.2019.125654

A specialized DNA extraction method and a SYBR Green quantitative polymerase chain reaction (SyG-qPCR) assay were combined to generate a ready-to-use kit for rapid detection of porcine admixtures in processed meat products. Our qPCR assay utilized repetitive LINE-1 elements specific to the genome of Sus scrofa domesticus (pig) as a target and incorporated internal controls. We improved the genomic DNA extraction method, and reduced extraction times to the minimum. The method was validated for specificity, sensitivity (0.001% w/w) and robustness, and values were compared with those of a commercially available kit. We also tested our method using 121 processed food products and consistently detected amplification only in samples containing pork. Due to its efficiency and cost-effectiveness, our method represents a valuable new method for detecting food adulteration with pork that is superior to existing quality control approaches.

Matched MeSH terms: DNA/analysis*; DNA/isolation & purification; DNA/standards
Kesan psikostres terhadap kerosakan dna dan ketaknormalan titisan sitoplasma sperma manusia

Norhamizan Hashim, Khairul Osman, Siti Fatimah Ibrahim, Rosliah Harun, Rafeah Pakri Mohamed

Sains Malaysiana, 2016;45:1931-1938.

Ketidaksuburan idiopati dalam kalangan lelaki telah dikaitkan dengan kesan psikostres. Walaupun begitu, hubungan langsung antara psikostres dan ketaknormalan kualiti semen masih samar. Maka, kajian ini dijalankan untuk menentukan kesan psikostres terhadap kualiti semen terutama kesan berdasarkan residu sitoplasma dan kerosakan DNA sperma. Dalam kajian ini, responden lelaki berumur antara 25-45 tahun dipilih secara rawak dalam kalangan pesakit yang mendapatkan rawatan di Pusat Kesuburan Lembaga Penduduk dan Pembangunan Keluarga Negara (LPPKN). Seramai 331 responden akhirnya telah dipilih daripada 628 responden selepas mengambil kira faktor penolakan. Setiap responden perlu menjawab borang keizinan dan soal selidik GHQ-12 bagi penentuan tahap stres sebelum pengambilan sampel semen mengikut piawaian WHO (2010). Tahap stres diukur berdasarkan keadaan semasa responden dalam tempoh 3-4 minggu sebelum kajian. Analisis semen, pewarnaan papanicolau dan asai komet neutral digunakan untuk penentuan kualiti semen dan kerosakan DNA sperma. Keputusan menunjukkan tidak terdapat hubungan yang signifikan antara psikostres dan ketaknormalan residu sitoplasma (U=895.50, p=0.08). Namun begitu, psikostres memberi kesan kepada peratus morfologi normal (U=6317.50, p<0.05) dan kerosakan DNA sperma (U=1047.00, p<0.01). Kesimpulannya, psikostres kronik boleh menjejaskan kualiti semen dan kerosakan DNA sperma serta mempengaruhi kesuburan.

Matched MeSH terms: DNA

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