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Fulltext Advances in pulmonary drug delivery targeting microbial biofilms in respiratory diseases

Sharma A, Kumar D, Dahiya K, Hawthorne S, Jha SK, Jha NK, et al.

Nanomedicine (Lond), 2021 09;16(21):1905-1923.
PMID: 34348474 DOI: 10.2217/nnm-2021-0057

The increasing burden of respiratory diseases caused by microbial infections poses an immense threat to global health. This review focuses on the various types of biofilms that affect the respiratory system and cause pulmonary infections, specifically bacterial biofilms. The article also sheds light on the current strategies employed for the treatment of such pulmonary infection-causing biofilms. The potential of nanocarriers as an effective treatment modality for pulmonary infections is discussed, along with the challenges faced during treatment and the measures that may be implemented to overcome these. Understanding the primary approaches of treatment against biofilm infection and applications of drug-delivery systems that employ nanoparticle-based approaches in the disruption of biofilms are of utmost interest which may guide scientists to explore the vistas of biofilm research while determining suitable treatment modalities for pulmonary respiratory infections.

Matched MeSH terms: Biofilms
Fulltext Biofilm formation following chitosan-based varnish or chlorhexidine-fluoride varnish application in patients undergoing fixed orthodontic treatment: a double blinded randomised controlled trial

Poornima P, Krithikadatta J, Ponraj RR, Velmurugan N, Kishen A

BMC Oral Health, 2021 09 23;21(1):465.
PMID: 34556107 DOI: 10.1186/s12903-021-01805-8

BACKGROUND: Orthodontic treatment poses an increased risk of plaque accumulation and demineralisation of enamel leading to white spot lesion around the brackets. This parallel arm trial aims to assess the degree of bacterial plaque formation adjacent to orthodontic brackets, following the application of a chitosan-based varnish or chlorhexidene-fluoride varnish.
METHODS: A total of 200 teeth from 20 patients undergoing fixed orthodontic therapy were assessed and biofilm formation around the brackets were recorded using the Bonded Bracket Index (Plaque index) at baseline and weekly for 6 weeks. The bacterial count and plaque pH at corresponding weekly intervals were also recorded. Following bracket bonding, the patients were cluster randomised to receive chitosan-based varnish-CHS (UNO Gel Bioschell, Germiphene corp., Brantford, Canada) or chlorhexidine-fluoride varnish-CFV (Cervitec F, Ivoclar Vivadent, Schaan, Liechtenstein) every week on the representative teeth respectively. BBI proportions were compared between groups at all time intervals using Chi square test. Mean plaque bacterial count and plaque pH were compared using Mann Whitney U test and Tukey's HSD test respectively.
RESULTS: Baseline characteristics were similar between the groups: Mean age was CHS = 23 and CFV = 21; male to female ratio was CHS = 5/5, CFV = 7/3. At the end of 6 weeks, chitosan-based varnish performed equal to chlorhexidine-fluoride varnish (P > 0.05) with 98% and 95% of teeth with acceptable scores respectively. The plaque bacterial count significantly reduced at 6 weeks for both varnish compared to the baseline; The value for CHS was 0.43 ± 0.4 × 104 and CFV was 0.77 ± 0.64 × 104 CFU (P

Matched MeSH terms: Biofilms
Fulltext Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces

Tang, P.L., Pui, C.F., Wong, W.C., Noorlis, A., Son, R.

International Food Research Journal, 2012;19(1):71-76.
MyJurnal

This study aimed to determine the biofilm formation ability by Salmonella Typhi on cucumber, mango and guava surface, as well as to determine the relationship between time contact and biofilm formation. Crystal violet assay was performed to quantify the biofilm formation based on the value of optical density at 570 nm of the destaining crystal violet at the specific interval time. The result showed that the attachment of the bacterial cells on the fresh produce surface increased with the contact time. The readings of OD570at time 12 h for cucumber, mango and guava surfaces were 0.824, 0.683 and 0.598, respectively, indicating that the biofilm formation by Salmonella Typhi on different fresh produce surface varied with time. Since the result showed that Salmonella Typhi formed biofilm on fresh produce surfaces, hygienic practice from farm to fork including handling, processing, distribution and storage of the fresh produce should be of concern.

Matched MeSH terms: Biofilms
Microbiome involved in microbial electrochemical systems (MESs): A review

Saratale RG, Saratale GD, Pugazhendhi A, Zhen G, Kumar G, Kadier A, et al.

Chemosphere, 2017 Mar 09;177:176-188.
PMID: 28288426 DOI: 10.1016/j.chemosphere.2017.02.143

Microbial electrochemical systems (MESs) are an attracting technology for the disposal of wastewater treatment and simultaneous energy production. In MESs, at the anode microorganisms through the catalytic activity generates electrons that can be converted into electricity or other valuable chemical compounds. Microorganisms those having ability to donate and accept electrons to and from anode and cathode electrodes, respectively are recognized as 'exoelectrogens'. In the MESs, it renders an important function for its performance. In the present mini-review, we have discussed the role of microbiome including pure culture, enriched culture and mixed culture in different BESs application. The effects of operational and biological factors on microbiome development have been discussed. Further discussion about the molecular techniques for the evaluation of microbial community analysis is addressed. In addition different electrochemical techniques for extracellular electron transfer (EET) mechanism of electroactive biofilms have been discussed. This review highlights the importance of microbiome in the development of MESs, effective operational factors for exo-electrogens activities as well their key challenges and future technological aspects are also briefly discussed.

Matched MeSH terms: Biofilms
Fulltext Antimicrorobial evaluation of sodium hypochlorite and ozonated water on E. faecalis biofilm

Ibrahim, N.Z., Abdullah, M.

Ann Dent, 2008;15(1):20-26.
MyJurnal

This study aim to evaluate antimicrobial efficacy of sodium hypochlorite (NaOCl) and ozonated water against Enterococci faecalis biofilm. The bacterial biofilm was exposed to 2.62%, 1.31% NaOCl and 0.1 ppm ozonated water over a range of time periods. The presence of viable cells was determined by enumeration of colony forming units (CFU). All experiments were repeated four times (n=4). The effectiveness of the agents was compared using nonparametric Kruskal- Wallis test. The result revealed that 2.62% of NaOCl can completely kill E. faecalis biofilm in 15 minutes whereas 1.31 % NaOCl required a longer time to produce such effect. 0.1 ppm ozonated, however, did not exhibit any antimicrobial effect within the period of time tested. From this study, it can be concluded that 0.1 ppm ozonated water was not comparable with 2.62% and 1.31% NaOCl in antimicrobial efficacy against E. faecalis biofilm.

Matched MeSH terms: Biofilms
Fulltext The evaluation of Streptococcus mutans content of saliva in patients with recurrent oral ulceration

Al-Ahmad, Basma Ezzat Mustafa, Muhannad Ali Kashmoola, Nur Aini Zakaria

International Medical Journal Malaysia, 2017;16(21):14-14.
MyJurnal

Recurrent oral ulcer is a chronic inflammatory condition characterized by mucosa
ulceration, which was believed to have bacterial etiology. Streptococcus mutans is one of the
bacteria which have been implicated in this case; it is gram-positive bacteria that reside in the
biofilms on the tooth surfaces. The aim of this study was to evaluate Streptococcus mutans content
in saliva of recurrent oral ulcer patients and its role as a prognostic factor. (Copied from article).

Matched MeSH terms: Biofilms
Fulltext Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of Orthosiphon Stamineus and Andrographis Paniculata leaves and stems

Farah Adiba Yang Abd Talib, Hairul Aini Hamzah, Mohammed Imad Al-Deen Mustafa Mahmoud

International Medical Journal Malaysia, 2017;16(11):32-32.
MyJurnal

Quorum sensing controls a wide spectrum of processes and phenotypic
behaviours including biofilm formation that helps in the survival and virulence of
bacteria in hosts. Thus, anti-quorum sensing is suggested to combat bacterial infections.
This study aimed to evaluate the anti-biofilm forming and anti-quorum sensing activities
which may contribute to the anti-bacterial effect of ethanol and aqueous extracts of
Orthosiphon stamineus and Andrographis paniculata leaves and stems. (Copied from article).

Matched MeSH terms: Biofilms
Fulltext Natural Therapeutic Options in Endodontics - A Review

Venkateshbabu N, Anand S, Abarajithan M, Sheriff SO, Jacob PS, Sonia N

Open Dent J, 2016;10:214-26.
PMID: 27386007 DOI: 10.2174/1874210601610010214

Complete eradication of microbial biofilms and elimination of the smear layer are the key factors during endodontic treatment. Various chemical irrigants have been proposed in the literature for the same. The major setback with these chemical irrigants is that they are not bio-friendly to the dental and peri-radicular tissues. In the recent years, research to use natural products for root canal disinfection has gained importance. The aim of this article is to compile various herbal products that have been used as an irrigants and intracanal medicaments in the field of Endodontics to eradicate the biofilm and remove smear layer.

Matched MeSH terms: Biofilms
Fulltext Cloning and Characterization of the Autoinducer Synthase Gene from Lipid-Degrading Bacterium Cedecea neteri

Tan KH, How KY, Tan JY, Yin WF, Chan KG

Front Microbiol, 2017;8:72.
PMID: 28197135 DOI: 10.3389/fmicb.2017.00072

The process of intercellular communication among bacteria, termed quorum sensing (QS), is mediated by small diffusible molecules known as the autoinducers. QS allows the population to react to the change of cell density in unison, in processes such as biofilm formation, plasmid conjugation, virulence, motility and root nodulation. In Gram-negative proteobacteria, N-acyl homoserine lactone (AHL) is the common "language" to coordinate gene expression. This signaling molecule is usually synthesized by LuxI-type proteins. We have previously discovered that a rare bacterium, Cedecea neteri, exhibits AHL-type QS activity. With information generated from genome sequencing, we have identified the luxIR gene pair responsible for AHL-type QS and named it cneIR. In this study, we have cloned and expressed the 636 bp luxI homolog in an Escherichia coli host for further characterization. Our findings show that E. coli harboring cneI produced the same AHL profile as the wild type C. neteri, with the synthesis of AHL known as N-butyryl-homoserine lactone. This 25 kDa LuxI homolog shares high similarity with other AHL synthases from closely related species. This work is the first documentation of molecular cloning and characterization of luxI homolog from C. neteri.

Matched MeSH terms: Biofilms
Fulltext Transcriptomic and Genomic Approaches for Unravelling Candida albicans Biofilm Formation and Drug Resistance-An Update

Chong PP, Chin VK, Wong WF, Madhavan P, Yong VC, Looi CY

Genes (Basel), 2018 Nov 07;9(11).
PMID: 30405082 DOI: 10.3390/genes9110540

Candida albicans is an opportunistic fungal pathogen, which causes a plethora of superficial, as well as invasive, infections in humans. The ability of this fungus in switching from commensalism to active infection is attributed to its many virulence traits. Biofilm formation is a key process, which allows the fungus to adhere to and proliferate on medically implanted devices as well as host tissue and cause serious life-threatening infections. Biofilms are complex communities of filamentous and yeast cells surrounded by an extracellular matrix that confers an enhanced degree of resistance to antifungal drugs. Moreover, the extensive plasticity of the C. albicans genome has given this versatile fungus the added advantage of microevolution and adaptation to thrive within the unique environmental niches within the host. To combat these challenges in dealing with C. albicans infections, it is imperative that we target specifically the molecular pathways involved in biofilm formation as well as drug resistance. With the advent of the -omics era and whole genome sequencing platforms, novel pathways and genes involved in the pathogenesis of the fungus have been unraveled. Researchers have used a myriad of strategies including transcriptome analysis for C. albicans cells grown in different environments, whole genome sequencing of different strains, functional genomics approaches to identify critical regulatory genes, as well as comparative genomics analysis between C. albicans and its closely related, much less virulent relative, C. dubliniensis, in the quest to increase our understanding of the mechanisms underlying the success of C. albicans as a major fungal pathogen. This review attempts to summarize the most recent advancements in the field of biofilm and antifungal resistance research and offers suggestions for future directions in therapeutics development.

Matched MeSH terms: Biofilms
Fulltext Mechanical and physical properties of gellan gum (GG) biofilm: effect of glycerol

Razali, M.H., Ismail, N.A., Osman, U.M., Amin, K.A.M.

ASM Science Journal, 2018;11(101):158-165.
MyJurnal

The aim of this work was to investigate the effect of glycerol concentration on mechanical
and physical properties of gellan gum (GG) biofilm. The biofilm was prepared using solvent
casting method and the effective glycerol concentration was found to be within 30-50%
w/w (based on GG weight). At 60 and 70 w/w% of glycerol, the films started to distort
because the films was flexible and brittle. As glycerol concentration was increased the tensile
strength (TS) and Youngs modulus (E) of films decreased. Somehow, elongation at break
(EAB), water vapor transmission rate (WVTR) and swelling of films was increased. Glycerol
plasticized GG biofilm was thermally stable and flexible, proposed its can be exploited as
film-forming material and with optimized glycerol concentration it has good mechanical and
physical properties for edible biofilm.

Matched MeSH terms: Biofilms
Nicotine enhances the thickness of biofilm and adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019

Gunasegar S, Himratul-Aznita WH

FEMS Yeast Res., 2019 Mar 01;19(2).
PMID: 30476044 DOI: 10.1093/femsyr/foy123

Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 hyphal-wall protein 1 (HWP1) are involved in hyphae formation and pathogenesis. The transcriptional agglutinin-like sequence 3 (ALS3) genes in both species are responsible for the development of biofilm and colonization on tooth surfaces. Therefore, we investigated the expression of HWP1 and ALS3 quantitatively in C. albicans and C. parapsilosis and examined the biofilm structure upon exposure to various nicotine concentrations. In vitro, biofilms of Candida species were developed directly on slides using the Lab-Tek Chamber Slide System and visualized by confocal laser scanning microscopy. Quantitative real-time polymerase chain reaction was used to measure HWP1 and ALS3 expression in C. albicans ATCC 14053 and C. parapsilosis ATCC 22019. The results indicated that nicotine multiplied the number of yeast cells and increased the extracellular polysaccharides of Candida species. We also found that 1-2 mg/mL nicotine could enhance the formation of biofilm. The findings also revealed that the expression of HWP1 and ALS3 in Candida species were increased as the nicotine concentration increased. Therefore, nicotine influences the biofilm development of oral-associated C. albicans ATCC 14053 and C. parapsilosis ATCC 22019.

Matched MeSH terms: Biofilms
Effect of aquaculture salinity on nitrification and microbial community in moving bed bioreactors with immobilized microbial granules

Gao Y, Wang X, Li J, Lee CT, Ong PY, Zhang Z, et al.

Bioresour Technol, 2020 Feb;297:122427.
PMID: 31784249 DOI: 10.1016/j.biortech.2019.122427

The novel immobilized microbial granules (IMG) shows a significant effect of nitrification for freshwater aquaculture. However, there is lack of evaluation study on the performance of nitrification at high salinity due to the concentration of recycled water or seawater utilization. A laboratory scale moving bed bioreactor (MBBR) with IMG was tested on recycled synthetic aquaculture wastewater for the nitrification at 2.5 mg/L NH3-N daily. The results indicated that IMG showed a high salinity tolerance and effectively converted ammonia to nitrate up to 92% at high salinity of 35.0 g/L NaCl. As salinity increased from near zero to 35.0 g/L, the microbial activity of nitrite oxidation bacteria (NOB) in the IMG decreased by 86.32%. The microbial community analysis indicated that salinity significantly influenced the community structure. It was found that Nitrosomonas sp. and Nitrospira sp. were the dominant genera for ammonia oxidation bacteria (AOB) and NOB respectively at different salinity levels.

Matched MeSH terms: Biofilms
Effects of Salvadora persicaExtract on the bacterial population in single-species biofilm

Nordin Fatin-Majdina, Haji Abd Rahim Zubaidah, Mohd Bakri Marina, Abd Razak Monica@Munirah

Sains Malaysiana, 2014;43:1889-1893.

The effect of S. persica extract on the population of bacteria in single-species biofilm was investigated. An artificial mouth (NAM) system was used for the development of biofilm. In one experiment, aqueous extract of S. persica at sub-MIC concentration (5 mg/mL) was first pumped into the NAM system to treat the experimental pellicle on glass beads before inoculating it with the bacteria (Strep. mitis, Strep. mutans and Strep. sanguinis). This would resemble using the aqueous extract of S. persica before meals. In another experiment, the aqueous extract of S. persica was pumped into the NAM system after the bacterial inoculation and this would resemble using it after meals. In both experiments, 24 h biofilms were allowed to form. The bacterial population of the biofilms was determined and expressed as colony forming unit per mL (cfu mL-1). For negative control, sterilized distilled water was used whereas for positive control, commercial Listerine. It was found that the population of Strep. mitis, Strep. mutans and Strep. sanguinis in the respective biofilms for both experiments involving treatment with S. persica extract or Listerine was significantly reduced by more than 70% (p<0.05) when compared with the negative control. Comparing the effect of S. persica with Listerine on the bacterial population of the biofilms when used either before or after meal showed that S. persica is slightly less effective towards S. sanguinis before meal and towards S. mutans after meal (p<0.05). This study suggests that the efficiency of the antibacterial effect of S. persica is species-related and by its way of usage.

Matched MeSH terms: Biofilms
Inhibition of consortium sulfate reducing bacteria from crude oil for carbon steel protection

Nur Akma Mahat, Norinsan Kamil Othman, Mohd Nazri Idris, Fathul Karim Sahrani

Sains Malaysiana, 2015;44:1587-1591.

The efficiency of cetyltrimethylammonium bromide (CTAB) to reduce the activity of consortium bacteria consisting of
sulphate-reducing bacteria (C-SRB) has been investigated on variable concentration by weight loss test, potentiodynamic
polarization and diffusion disk methods. C-SRB was isolated from tropical crude oil of Malaysian offshore. Biofilm analysis
was also evaluated by variable pressure scanning electron microscopy (VPSEM). Weight loss and potentiodynamic
polarization analyses showed that CTAB is able to inhibit the biocorrosion process and their inhibition efficiency had
reached to 85 and 65% at 300 ppm CTAB, respectively. Increasing of CTAB efficiency as a function of concentration was
also supported by diffusion disk analysis. Biofilm analysis showed that less of C-SRB and their metabolic by-product had
been observed. It was concluded that CTAB was able to reduce the C-SRB activity and prevent biocorrosion process on
carbon steel surface.

Matched MeSH terms: Biofilms
An in vitro study of a novel quaternary ammonium silane endodontic irrigant

Daood U, Parolia A, Elkezza A, Yiu CK, Abbott P, Matinlinna JP, et al.

Dent Mater, 2019 09;35(9):1264-1278.
PMID: 31201019 DOI: 10.1016/j.dental.2019.05.020

OBJECTIVE: To analyze effect of NaOCl+2% quaternary ammonium silane (QAS)-containing novel irrigant against bacteria impregnated inside the root canal system, and to evaluate its antimicrobial and mechanical potential of dentine substrate.
METHODS: Root canal was prepared using stainless steel K-files™ and ProTaper™ and subjected to manual and ultrasonic irrigation using 6% NaOCl+2% CHX, 6% NaOCl+2% QAS and saline as control. For confocal-microscopy, Raman spectroscopy and SEM analysis before and after treatment, Enterococcus faecalis cultured for 7 days. Raman spectroscopy analysis was done across cut section of gutta percha/sealer-dentine to detect resin infiltration. Indentation of mechanical properties was evaluated using a Berkovich indenter. The contact angle of irrigants and surface free energy were evaluated. Mineralization nodules were detected through Alazarin red after 14 days.
RESULTS: Control biofilms showed dense green colonies. Majority of E. faecalis bacteria were present in biofilm fluoresced red in NaOCl+2% QAS group. There was reduction of 484cm-1 Raman band and its intensity reached lowest with NaOCl+2% QAS. There was an increase in 1350-1420cm-1 intensity in the NaOCl+2% CHX groups. Gradual decrease in 1639cm-1 and 1609cm-1 Raman signal ratios were seen in the resin-depth region of 17μm>, 14.1μm> and 13.2μm for NaOCl+2% QAS, NaOCl+2% CHX and control groups respectively. All obturated groups showed an intact sealer/dentine interface with a few notable differences. 0.771 and 83.5% creep indentation distance for NaOCl+2% QAS ultrasonic groups were observed. Highest proportion of polar component was significantly found in the NaOCl+2% QAS groups which was significantly higher as compared to other groups. Mineralized nodules were increased in NaOCl+2% QAS.
SIGNIFICANCE: Favorable antimicrobial and endodontic profile of the NaOCl+2% QAS solution might suggest clinical use for it for more predictable reduction of intracanal bacteria.

Matched MeSH terms: Biofilms
Recent progress in integrated fixed-film activated sludge process for wastewater treatment: A review

Waqas S, Bilad MR, Man Z, Wibisono Y, Jaafar J, Indra Mahlia TM, et al.

J Environ Manage, 2020 Aug 15;268:110718.
PMID: 32510449 DOI: 10.1016/j.jenvman.2020.110718

Integrated fixed-film activated sludge (IFAS) process is considered as one of the leading-edge processes that provides a sustainable solution for wastewater treatment. IFAS was introduced as an advancement of the moving bed biofilm reactor by integrating the attached and the suspended growth systems. IFAS offers advantages over the conventional activated sludge process such as reduced footprint, enhanced nutrient removal, complete nitrification, longer solids retention time and better removal of anthropogenic composites. IFAS has been recognized as an attractive option as stated from the results of many pilot and full scales studies. Generally, IFAS achieves >90% removals for combined chemical oxygen demand and ammonia, improves sludge settling properties and enhances operational stability. Recently developed IFAS reactors incorporate frameworks for either methane production, energy generation through algae, or microbial fuel cells. This review details the recent development in IFAS with the focus on the pilot and full-scale applications. The microbial community analyses of IFAS biofilm and floc are underlined along with the special emphasis on organics and nitrogen removals, as well as the future research perspectives.

Matched MeSH terms: Biofilms
Fulltext Physical, mechanical, chemical and biological properties data of gellan gum incorporating titanium dioxide nanoparticles biofilm

Razali MH, Ismail NA, Amin KAM

Data Brief, 2020 Jun;30:105478.
PMID: 32346560 DOI: 10.1016/j.dib.2020.105478

Gellan gum incorporating titanium dioxide nanoparticles biofilm was synthesized and characterized using UV, FTIR and XRD to study their physical and chemical properties. The mechanical properties were measured using universal mechanical testing. Meanwhile, the biological properties were investigated towards for antibacterial and cell proliferation. This comprehensive data are relevant with the research article entitled "Gellan gum incorporating titanium dioxide nanoparticles biofilm as wound dressing: Physicochemical, mechanical, antibacterial properties and wound healing studies" [1].

Matched MeSH terms: Biofilms
Fulltext Characterisation of Bacterial Isolates from Infected Post-Operative Patients in a Malaysian Tertiary Heart Care Centre

Yong YK, Wen NCM, Yeo GEC, Chew ZX, Chan LL, Md Zain NZ, et al.

Int J Environ Res Public Health, 2021 09 17;18(18).
PMID: 34574752 DOI: 10.3390/ijerph18189828

Several bacterial species cause post-operative infections, which has been a critical health concern among hospital patients. Our study in this direction is a much-needed exploratory study that was carried out at the National Heart Institute (IJN) of Malaysia to examine the virulence properties of causative bacteria obtained from postoperative patients. The bacterial isolates and data were provided by the IJN. Antibiotic resistance gene patterns, and the ability to form biofilm were investigated for 127 isolates. Klebsiella pneumoniae (36.2%) was the most common isolate collected, which was followed by Pseudomonas aeruginosa (26%), Staphylococcus aureus (23.6%), Streptococcus spp. (8.7%) and Acinetobacter baumannii (5.5%). There were 49 isolates that showed the presence of multidrug resistance genes. The mecA gene was surprisingly found in methicillin-susceptible S. aureus (MSSA), which also carried the ermA gene from those erythromycin-susceptible strains. The phenotypic antibiotic resistance profiles varied greatly between isolates. Findings from the biofilm assay revealed that 44 of the 127 isolates demonstrated the ability to produce biofilms. Our findings provide insights into the possibility of some of these bacteria surviving under antibiotic stress, and some antibiotic resistance genes being silenced.

Matched MeSH terms: Biofilms
Molecular identification and biofilm-forming ability of Elizabethkingia species

Puah SM, Fong SP, Kee BP, Puthucheary SD, Chua KH

Microb Pathog, 2022 Jan;162:105345.
PMID: 34896547 DOI: 10.1016/j.micpath.2021.105345

Recently, Elizabethkingia species have gained attention as a cause of life-threatening infections. The identification via phenotypic methods of three important species- Elizabethkingia meningoseptica, E. anophelis and E. miricola is difficult. Our objectives were to re-assess 30 archived Flavobacterium meningosepticum isolates using 16S rRNA gene sequencing, ERIC-PCR, and biofilm formation assay. Twenty-four isolates were re-identified as E. anophelis and 6 as E. miricola. All of them had the ability to form biofilm as shown in microtiter plate assay based on crystal violet staining. Overall, E. anophelis had a higher specific biofilm formation index compared to E. miricola. A total of 42% (10 out of 24) of E. anophelis were classified as strong, 29% (7 out of 24) as moderate and 29% (7 out of 24) as weak biofilm producers. E. miricola, 17% (1 out of 6) isolates were strong biofilm producers, 50% (3 out of 6) moderate and 33% (2 out of 6) were weak producers. E. anophelis from tracheal secretions were significantly associated with (p = 0.0361) strong biofilm formation. In summary, this study showed that the isolates originally identified as F. meningosepticum were re-classified using the 16S rRNA gene as one of two Elizabethkingia species. The ability of E. anophelis to form strong biofilm in endotracheal tubes indicates their probable role in the pathogenesis of Elizabethkingia infections.

Matched MeSH terms: Biofilms

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