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  1. A genome-wide characterization of copy number variations in native populations of Peninsular Malaysia
    Fu R, Mokhtar SS, Phipps ME, Hoh BP, Xu S
    Eur J Hum Genet, 2018 06;26(6):886-897.
    PMID: 29476164 DOI: 10.1038/s41431-018-0120-8
    Copy number variations (CNVs) are genomic structural variations that result from the deletion or duplication of large genomic segments. The characterization of CNVs is largely underrepresented, particularly those of indigenous populations, such as the Orang Asli in Peninsular Malaysia. In the present study, we first characterized the genome-wide CNVs of four major native populations from Peninsular Malaysia, including the Malays and three Orang Asli populations; namely, Proto-Malay, Senoi, and Negrito (collectively called PM). We subsequently assessed the distribution of CNVs across the four populations. The resulting global CNV map revealed 3102 CNVs, with an average of more than 100 CNVs per individual. We identified genes harboring CNVs that are highly differentiated between PM and global populations, indicating that these genes are predominantly enriched in immune responses and defense functions, including APOBEC3A_B, beta-defensin genes, and CCL3L1, followed by other biological functions, such as drug and toxin metabolism and responses to radiation, suggesting some attributions between CNV variations and adaptations of the PM groups to the local environmental conditions of tropical rainforests.
    Matched MeSH terms: DNA Copy Number Variations
  2. Fulltext Morphological Characteristics and Habitats of Red Seaweed Gracilaria spp. (Gracilariaceae, Rhodophyta) in Santubong and Asajaya, Sarawak, Malaysia
    Othman MNA, Hassan R, Harith MN, Sah ASRM
    Trop Life Sci Res, 2018 Mar;29(1):87-101.
    PMID: 29644017 MyJurnal DOI: 10.21315/tlsr2018.29.1.6
    Red seaweed Gracilaria, one of the largest genus in Division Rhodophyta inhabits Sarawak coastal water. This study was designed to identify the species of Gracilaria using morphological approach and to assess selected water quality parameters in Gracilaria habitats. Three field samplings were carried out in Santubong and Asajaya, Sarawak from November 2013 to December 2014. Overall, three species were identified namely Gracilaria changii, G. blodgettii and G. coronopifolia, attached to net of cage culture in Santubong and root of mangrove trees in Asajaya. In addition, three different taxa of aquatic macroinvertebrates (polychaete, small crab, bivalve) and single species of red seaweed (Acanthophora sp.) were observed in Gracilaria assemblages. An estimate of 37% to 40% of the upper part of the cage net in Santubong was covered by seaweeds and only 16% to 20% in Asajaya's mangrove. The study had provided better information on identification of Gracilaria and their habitat in Sarawak. Future work involving DNA barcoding of each species is in progress.
    Matched MeSH terms: DNA Barcoding, Taxonomic
  3. Fulltext Prediction of clinical symptoms of Schizophrenia based on COMT Methylation marker
    Nour El Huda Abd Rahim, Mohd Nabil Fikri Rahim, Norsidah Ku Zaifah, Hanisah Mohd Noor, Kartini Abdullah, Norlelawati A. Talib
    International Medical Journal Malaysia, 2017;16(11):19-19.
    MyJurnal
    The dopamine hypothesis of schizophrenia is based on the fact that hyperdopaminergic
    state is involved in causing psychosis and antipsychotic drugs block the
    dopamine receptor. COMT regulates the homeostatic levels of neurotransmitter
    dopamine in the synapses and plays a role in the neurocognitive function. The
    dysregulation of dopamine in the prefrontal cortex influences the cognitive function and
    the severity of the psychotic symptoms in schizophrenia. During epigenetic event,
    methylated COMT gene may cause reduction in its expression and contribute to the
    clinical presentation of schizophrenia. Therefore, the aim of this study was to assess the
    feasibility of using COMT DNA methylation for the prediction of specific psychotic
    presentation of schizophrenia. (Copied from article).
    Matched MeSH terms: DNA Methylation
  4. Fulltext Silicon nanowire interface circuit for DNA detection
    Kamilu Iman Usman, Mohd Nizar Hamidon, Siti Fatimah Abd Rahman, Guan Ling Sim
    Pertanika Journal of Science & Technology, 2017;25(102):251-258.
    MyJurnal
    Detection and quantification of DNA is critical to many areas of life sciences and health care, from
    disease diagnosis to drug screening. The transduction of DNA through electrochemical methods have a fast response rate and with a conductometric device like the silicon nanowire which can be fabricated to have a similar diameter of the DNA molecule being targeted, detection is real-time. Critical to this is the interfacing of a current-source and an amplifier capable of achieving a maximum of 10 pico ampere input bias. In this project, we fabricated a silicon nanowire using the top down approach and built a circuit that can mimic the output signal as low as 12 nA and achieved a gain of 1 million to be interfaced with the nanowire for real-time DNA detection.
    Matched MeSH terms: DNA
  5. Fulltext Molecular characterisation of grouper iridovirus isolates from Peninsular Malaysia
    Hassan, M.D., Hazeri, M., Omar, A.R., Abba, Y., Allaudin, Z.N., Soltani, M., et al.
    Jurnal Veterinar Malaysia, 2017;29(1):1-6.
    MyJurnal
    Grouper Iriovirus (GIV) is one of the most devastating viral diseases of marine and cultured groupers worldwide. In the current study, 5 presumptive Malaysian GIV isolates were characterised through PCR amplification of the major capsid protein (MCP) gene and phylogenetic analysis of the sequences. The sequences from the five GIV isolates showed 100% homology with each other and a close relationship with grouper iridovirus isolate (GIV_Tn_352), which was clustered in group 1 together with King grouper iridovirus isolate (KGIV_Cy_346), Singapore grouper iridovirus (SGIV), and Crimson snapper iridovirus isolate (CSIV). The phylogenetic tree also showed different degree of relatedness with other Ranavirus strains which were obtained from the blast of GIV MCP gene in the NCBI database. This study confirmed the GIV isolates from Malaysia are related to other isolates that were reported previously.
    Matched MeSH terms: DNA, Viral
  6. IRF5-mediated immune responses and its implications in immunological disorders
    Kaur A, Lee LH, Chow SC, Fang CM
    Int Rev Immunol, 2018;37(5):229-248.
    PMID: 29985675 DOI: 10.1080/08830185.2018.1469629
    Transcription factors are gene regulators that activate or repress target genes. One family of the transcription factors that have been extensively studied for their crucial role in regulating gene network in the immune system is the interferon regulatory factors (IRFs). IRFs possess a novel turn-helix turn motif that recognizes a specific DNA consensus found in the promoters of many genes that are involved in immune responses. IRF5, a member of IRFs has recently gained much attention for its role in regulating inflammatory responses and autoimmune diseases. Here, we discuss the role of IRF5 in regulating immune cells functions and how the dysregulation of IRF5 contributes to the pathogenesis of immune disorders. We also review the latest findings of potential IRF5 inhibitors that modulate IRF5 activity in the effort of developing therapeutic approaches for treating inflammatory disorders.
    Matched MeSH terms: DNA
  7. New scenario for speciation in the benthic dinoflagellate genus Coolia (Dinophyceae)
    Leaw CP, Tan TH, Lim HC, Teng ST, Yong HL, Smith KF, et al.
    Harmful Algae, 2016 05;55:137-149.
    PMID: 28073527 DOI: 10.1016/j.hal.2016.02.010
    In this study, inter- and intraspecific genetic diversity within the marine harmful dinoflagellate genus Coolia Meunier was evaluated using isolates obtained from the tropics to subtropics in both Pacific and Atlantic Ocean basins. The aim was to assess the phylogeographic history of the genus and to clarify the validity of established species including Coolia malayensis. Phylogenetic analysis of the D1-D2 LSU rDNA sequences identified six major lineages (L1-L6) corresponding to the morphospecies Coolia malayensis (L1), C. monotis (L2), C. santacroce (L3), C. palmyrensis (L4), C. tropicalis (L5), and C. canariensis (L6). A median joining network (MJN) of C. malayensis ITS2 rDNA sequences revealed a total of 16 haplotypes; however, no spatial genetic differentiation among populations was observed. These MJN results in conjunction with CBC analysis, rDNA phylogenies and geographical distribution analyses confirm C. malayensis as a distinct species which is globally distributed in the tropical to warm-temperate regions. A molecular clock analysis using ITS2 rDNA revealed the evolutionary history of Coolia dated back to the Mesozoic, and supports the hypothesis that historical vicariant events in the early Cenozoic drove the allopatric differentiation of C. malayensis and C. monotis.
    Matched MeSH terms: DNA, Ribosomal
  8. Fulltext Advances & challenges in leptospiral vaccine development
    Bashiru G, Bahaman AR
    Indian J Med Res, 2018 Jan;147(1):15-22.
    PMID: 29749356 DOI: 10.4103/ijmr.IJMR_1022_16
    Considerable progress has been made in the field of leptospiral vaccines development since its first use as a killed vaccine in guinea pigs. Despite the fact that the immunity conferred is restricted to serovars with closely related lipopolysaccharide antigen, certain vaccines have remained useful, especially in endemic regions, for the protection of high-risk individuals. Other conventional vaccines such as the live-attenuated vaccine and lipopolysaccharide (LPS) vaccine have not gained popularity due to the reactive response that follows their administration and the lack of understanding of the pathogenesis of leptospirosis. With the recent breakthrough and availability of complete genome sequences of Leptospira, development of novel vaccine including recombinant protein vaccine using reverse vaccinology approaches has yielded encouraging results. However, factors hindering the development of effective leptospiral vaccines include variation in serovar distribution from region to region, establishment of renal carrier status following vaccination and determination of the dose and endpoint titres acceptable as definitive indicators of protective immunity. In this review, advancements and progress made in LPS-based vaccines, killed- and live-attenuated vaccines, recombinant peptide vaccines and DNA vaccines against leptospirosis are highlighted.
    Matched MeSH terms: Vaccines, DNA
  9. Fulltext Expression and DNA methylation of SERK, BBM, LEC2 and WUS genes in in vitro cultures of Boesenbergia rotunda (L.) Mansf
    Karim R, Tan YS, Singh P, Khalid N, Harikrishna JA
    Physiol Mol Biol Plants, 2018 Sep;24(5):741-751.
    PMID: 30150851 DOI: 10.1007/s12298-018-0566-8
    The process of somatic embryogenesis and plant regeneration involve changes in gene expression and have been associated with changes in DNA methylation. Here, we report the expression and DNA methylation patterns of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK), BABY BOOM (BBM), LEAFY COTYLEDON 2 (LEC2) and WUSCHEL (WUS) in meristematic block of newly emerged shoots from rhizome, embryogenic and non-embryogenic calli, prolonged cell suspension culture, ex vitro leaf, and in vitro leaf of regenerated plants of Boesenbergia rotunda. Among all seven samples, based on qRT-PCR, the highest level of expression of SERK, BBM and LEC2 was in embryogenic callus, while WUS was most highly expressed in meristematic block tissue followed by embryogenic callus. Relatively lower expression was observed in cell suspension culture and watery callus for SERK, LEC2 and WUS and in in vitro leaf for BBM. For gene specific methylation determined by bisulfite sequencing data, embryogenic callus samples had the lowest levels of DNA methylation at CG, CHG and CHH contexts of SERK, LEC2 and WUS. We observed negative correlation between DNA methylation at the CG and CHG contexts and the expression levels of SERK, BBM, LEC2 and WUS. Based on our results, we suggest that relatively higher expression and lower level of DNA methylation of SERK, BBM, LEC2 and WUS are associated with somatic embryogenesis and plant regeneration in B. rotunda.
    Matched MeSH terms: DNA Methylation
  10. Molecular Detection of Porphyromonas gingivalis in Chronic Periodontitis Patients
    Kulkarni PG, Gosavi S, Haricharan PB, Malgikar S, Mudrakola DP, Turagam N, et al.
    J Contemp Dent Pract, 2018 Aug 01;19(8):992-996.
    PMID: 30150503
    AIM: In the current study, Porphyromonas gingivalis was identified in chronic periodontitis patients and healthy subjects by polymerase chain reaction (PCR) and its presence correlated with the severity of clinical periodontal parameters.

    MATERIALS AND METHODS: Subgingival plaque samples were collected with sterile curette and subjected to deoxyribonucleic acid (DNA) extraction and subsequent PCR for detection of P. gingivalis.

    RESULTS: Porphyromonas gingivalis was detected in 60% of patients of group II (pocket depth up to 5 mm), and in 93.33% of patients of group III (pocket depth more than 5 mm). One periodontally healthy subject in group I (probing depth < 3 mm) showed the presence of P. gingivalis.

    CONCLUSION: Detection frequency of bacterium increased significantly with increase in probing pocket depth (PPD), loss of attachment (LOA), and gingival index (GI).

    CLINICAL SIGNIFICANCE: Porphyromonas gingivalis is strongly associated with chronic periodontitis and its detection frequency positively correlates with the severity of periodontal destruction.

    Matched MeSH terms: DNA
  11. Fulltext Platelet Transcriptome-based approaches in the fight against dengue and other diseases
    Suppiah, J., Sakinah, S., Chan, S.Y., Wong, Y.P., Subbiah, S.K., Chee, H.Y., et al.
    Pertanika Journal of Science & Technology, 2018;26(3):933-946.
    MyJurnal
    Human platelets are anucleate cells that lack in deoxyribonucleic acid (DNA), thus hampering genomic study on them. However, the presence of their own messenger ribonucleic acid (mRNA) transcript allows functional study via the transcriptome approach. Transcriptome not only allows profiling of platelet but also aids in studying gene regulation in virus infections and other diseases that have an impact on platelets. Some viruses are known to affect the platelet either by causing a reduction or destruction. Dengue virus is one of the most postulated virus having such effect and frequently linked to platelet reduction. The transcriptome approach has a pivotal role in providing a deeper insight to link certain diseases and their effect on platelets. This review critically discusses role of platelet in dengue and other viral diseases of public health relevance, with a specific focus on the methods currently used in platelet transcriptome profiling.
    Matched MeSH terms: DNA
  12. Fulltext Data on degradome sequencing and analysis from mock-inoculated and Fusarium oxysporum treated leaves samples in Persicaria minor
    Samad AFA, Sajad M, Jani J, Murad AMA, Ismail I
    Data Brief, 2018 Oct;20:555-557.
    PMID: 30197911 DOI: 10.1016/j.dib.2018.08.034
    Degradome sequencing referred as parallel analysis of RNA ends (PARE) by modifying 5'-rapid amplification of cDNA ends (RACE) with deep sequencing method. Deep sequencing of 5' products allow the determination of cleavage sites through the mapping of degradome fragments against small RNAs (miRNA or siRNA) on a large scale. Here, we carried out degradome sequencing in medicinal plant, Persicaria minor, to identify cleavage sites in small RNA libraries in control (mock-inoculated) and Fusarium oxysporum treated plants. The degradome library consisted of both control and treated samples which were pooled together during library preparation and named as D4. The D4 dataset have been deposited at GenBank under accession number SRX3921398, https://www.ncbi.nlm.nih.gov/sra/SRX3921398.
    Matched MeSH terms: DNA, Complementary
  13. Fulltext Performance of 2 Polymerization Protocols Targeting Cloned Toxoplasma Parasites
    Hanafy NA, Badr MS, Nasr GM
    Open Access Maced J Med Sci, 2018 Sep 25;6(9):1577-1580.
    PMID: 30337968 DOI: 10.3889/oamjms.2018.400
    BACKGROUND: Toxoplasma gondii is a common parasitic infection of humans. Infection is usually mild. Serious complications can occur in pregnant and immunocompromised patients.

    AIM: The present study aims to investigate the performance of 2 different PCR protocols; real-time quantitative molecular assays (qPCR) and conventional molecular assays (cPCR), using 2 different sets of primers and by using cloned purified Toxoplasma genomic substances to be evaluated as reference samples.

    METHODS: The target DNA was provided in 8 different quantities.

    RESULTS: Amplification failure was reported only with the cPCR in samples of low concentrations using both primer sets. Quantitative PCR detected the 8 different dilutions of the purified Toxoplasma gondii using the 2 sets of primers while cPCR was sensitive to detect only 6 different dilutions.

    CONCLUSION: Generally real-time quantitative molecular assays, is easy to use method compared to conventional PCR assay and produces more reliable results within only one hour time but still the possible application of qPCRs in routine diagnosis necessitates analysis of a large number of clinical samples in further studies to make the proper choice.

    Matched MeSH terms: DNA
  14. Antigenic potential of a recombinant polyvalent DNA vaccine against pathogenic leptospiral infection
    Garba B, Bahaman AR, Zakaria Z, Bejo SK, Mutalib AR, Bande F, et al.
    Microb Pathog, 2018 Nov;124:136-144.
    PMID: 30138761 DOI: 10.1016/j.micpath.2018.08.028
    Leptospirosis is a serious epidemic disease caused by pathogenic Leptospira species. The disease is endemic in most tropical and sub-tropical regions of the world. Currently, there is no effective polyvalent vaccine for prevention against most of the circulating serovars. Moreover, development of an efficient leptospiral vaccine capable of stimulating cross-protective immune responses against a wide range of serovars remains a daunting challenge. This, in part, is associated with the extensive diversity and variation of leptospiral serovars from region to region. In this study, a multi-epitope DNA vaccine encoding highly immunogenic epitopes from LipL32 and LipL41 was designed using in-silico approach. The DNA encoding antigenic epitopes was constructed from conserved pathogenic Leptospira genes (LipL32 and LipL41). Immunization of golden Syrian hamsters with the multi-epitope chimeric DNA vaccine resulted in the production of both agglutinating and neutralizing antibodies as evidence by MAT and in-vitro growth inhibition tests respectively. The antibodies produced reacted against eight different serovars and significantly reduced renal colonization following in vivo challenge. The vaccine was also able to significantly reduce renal colonization which is a very important factor responsible for persistence of leptospires among susceptible and reservoir animal hosts. In conclusion, the leptospiral multi-epitope chimeric DNA vaccine can serve as a potentially effective and safe vaccine against infection with different pathogenic leptospiral serovars.
    Matched MeSH terms: Vaccines, DNA/administration & dosage; Vaccines, DNA/genetics; Vaccines, DNA/immunology*
  15. Fulltext Bacteriophages and their applications
    Thung, T.Y., Lee, E., Tan, C.W., Malcolm, T.T.H., New, C.Y., Ramzi, O.S.B., et al.
    Food Research, 2018;2(5):404-414.
    MyJurnal
    Bacteriophages are ubiquitous in our world, mainly in the oceans, soil, the water and food
    we consume. They can be used efficiently in modern biotechnology, as well as alternatives
    to antibiotics for many antibiotic resistant bacterial strains. Phages can be used as vehicles
    for vaccines both DNA and protein, for the detection of pathogenic bacterial strain, as biocontrol
    agents in agriculture and food industry. This review outlines the properties as well
    as the influence of different external physical and chemical factors like temperature and
    acidity on phage persistence. A better understanding of the complex problem of phage
    sensitivity to external factors may be useful for other researchers working with phages.
    Furthermore, the applications of bacteriophages were described in this paper as well.
    Matched MeSH terms: DNA
  16. Fulltext Detection of Tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review
    Che Engku Noramalina Che-Engku-Chik, Siti Sarah Othman, Helmi Wasoh, Nor Azah Yusof, Jaafar Abdullah, Mohd Hazani Mat Zaid
    Journal of Biochemistry, Microbiology and Biotechnology, 2016;4(2):16-21.
    MyJurnal
    Despite the continued effort globally made to control the growing case of Tuberculosis (TB), it
    continues to be regarded as the second deadliest disease after the HIV. There are various
    methods developed to diagnose TB, most of which having the criteria of sensitive, selective,
    cheap and portable to be used in robust applications. Even with the advancement in medication,
    the important keys including early stage diagnosis is yet to be considered. In diagnosing TB, the
    only technique remained as the gold standard method is the culturing method, which is the Acid
    Fast Bacilli (AFB) staining. On the other hand, molecular technique utilising Polymerase Chain
    Reaction (PCR) assay is preferred as a non-culturing method. Additionally, as molecular
    techniques become advanced, real-time PCR or quantitative PCR (qPCR) using multiple probes
    in one shot has raised interest among researchers, because it can skip the process of gel
    electrophoresis. Recently, researchers have been working on electrochemical DNA sensors
    which are sensitive, selective, rapid, cheap and can meet with point of care (POC) testing
    requirements to diagnose TB.
    Matched MeSH terms: DNA
  17. Fulltext Conjunctiva granuloma, a rare presentation of ocular leptospirosis presenting as conjunctiva granuloma: case series
    Yeoh, S.Y., Anhar, H.S., Chua, S.M., Raja, N.R.O., Nor, F.A.J., Azida, J.W.A.K.
    Medicine & Health, 2018;13(1):272-278.
    MyJurnal
    Diagnosis of ocular leptospirosis is challenging and requires a high index of suspicion of previous leptospiral infection and good laboratory support. This case series focuses on two young females with unilateral conjunctiva granuloma. To the best of our knowledge, these are the first two cases of ocular leptospirosis with conjunctiva granuloma. The definitive diagnosis of ocular leptospirosis was based on laboratory studies in which conjunctival biopsies in these two cases showed positive leptospira DNA. Retrospectively, the history was suggestive as both patients had exposure to leptospira organism. In conclusion, a diagnosis of ocular leptospirosis requires a high clinical suspicion index supported by mandatory laboratory investigations.
    Matched MeSH terms: DNA
  18. A New Application Using a Chromogenic Assay in a Plant Pathogen DNA Macroarray Detection System
    Wong MY, Smart CD
    Plant Dis, 2012 Sep;96(9):1365-1371.
    PMID: 30727148 DOI: 10.1094/PDIS-07-11-0593-SR
    A DNA macroarray was previously developed to detect major fungal and oomycete pathogens of solanaceous crops. To provide a convenient alternative for researchers with no access to X-ray film-developing facilities, specific CCD cameras or Chemidoc XRS systems, a chromogenic detection method with sensitivity comparable with chemiluminescent detection, has been developed. A fungal (Stemphylium solani) and an oomycete (Phytophthora capsici) pathogen were used to develop the protocol using digoxigenin (DIG)-labeled targets. The internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (rDNA), including ITS1, 5.8S rDNA, and ITS2, was used as the target gene and polymerase chain reaction amplified as in the previous protocol. Various amounts of species-specific oligonucleotides on the array, quantities of DIG-labeled ITS amplicon, and hybridization temperatures were tested. The optimal conditions for hybridization were 55°C for 2 h using at least 10 pmol of each species-specific oligonucleotide and labeled target at 10 ng/ml of hybridization buffer. Incubation of the hybridized array with anti-DIG conjugated alkaline phosphatase substrates, NBT/BCIP, produced visible target signals between 1 and 3 h compared with 1 h in chemiluminescent detection. Samples from pure cultures, soil, and artificially inoculated plants were also used to compare the detection using chemiluminescent and chromogenic methods. Chromogenic detection was shown to yield similar results compared with chemiluminescent detection in regard to signal specificity, duration of hybridization between the array and targets, and cost, though it takes 1 to 2 h longer for the visualization process, thus providing a convenient alternative for researchers who lack darkroom facilities. To our knowledge, this is the first report of DNA macroarray detection of plant pathogens using a chromogenic method.
    Matched MeSH terms: DNA, Ribosomal
  19. Fulltext A new species of Clavicornaltica (Coleoptera: Chrysomelidae), discovered and described on a field course to Kuala Belalong, Brunei
    Schilthuizen M, Berenyi AEA, Limin A, Brahim A, Cicuzza D, Eales AJ, et al.
    Biodivers Data J, 2019.
    PMID: 30740026 DOI: 10.3897/BDJ.7.e32555
    Background: Clavicornaltica is a genus of very small flea beetles living in the leaf litter layer of Asian forests, easily sampled with Winkler extraction. The genus is presumably very rich in species, but their taxonomy is hampered by their small size and morphological uniformity.

    New information: On a 'taxon expedition'-style field course at Kuala Belalong Field Studies Centre in Brunei Darussalam (Borneo), a new species, Clavicornaltica belalongensis n. sp., was discovered and taxonomically treated by the course participants. We also present the first DNA barcodes for the genus.

    Matched MeSH terms: DNA Barcoding, Taxonomic
  20. Fulltext Antioxidant, anticancer, apoptosis properties and chemical composition of black truffle Terfezia claveryi
    Dahham SS, Al-Rawi SS, Ibrahim AH, Abdul Majid AS, Abdul Majid AMS
    Saudi J Biol Sci, 2018 Dec;25(8):1524-1534.
    PMID: 30591773 DOI: 10.1016/j.sjbs.2016.01.031
    Desert truffles are seasonal and important edible fungi that grow wild in many countries around the world. Truffles are natural food sources that have significant compositions. In this work, the antioxidant, chemical composition, anticancer, and antiangiogenesis properties of the Terfezia claveryi truffle were investigated. Solvent extractions of the T. claveryi were evaluated for antioxidant activities using (DPPH, FRAP and ABTS methods). The extracts cytotoxicity on the cancer cell lines (HT29, MCF-7, PC3 and U-87 MG) was determined by MTT assay, while the anti-angiogenic efficacy was tested using ex-vivo assay. All extracts showed moderate anticancer activities against all cancer cells (p DNA fragmentation p 
    Matched MeSH terms: DNA Fragmentation
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