Displaying publications 1221 - 1240 of 3311 in total

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  1. Fulltext Prevention of brain hypoperfusion-induced neurodegeneration in rat’s hippocampus by black cumin fixed oil treatment
    Marwan Saad Azzubaidi, Al-Ani, Imad Matloub, Anil Kumar Saxena, Ghasak Ghazi Faisal
    International Medical Journal Malaysia, 2018;17(1):103-112.
    MyJurnal
    Introduction: The oil extract of black cumin seeds Nigella sativa (NSO) demonstrated considerable
    preservation of spatial cognitive functions in rats subjected to chronic brain hypoperfusion (CBH). The hippocampal CA1 region pyramidal cells are the earliest neurons suffering neurodegeneration following CBH. Objective: The current study was devoted to assess the protective effects of Nigella sativa (NSO) treatment on CA1 hippocampal pyramidal cells of rats subjected to chronic brain hypoperfusion (CBH) that was achieved through permanent two vessel occlusion (2VO) procedure. Methods: Twenty four rats were equally divided into three groups; sham control, untreated 2VO and NSO treated group (2VO with daily oral NSO treatment. After the 10th postoperative week coronal sections of the hippocampus were collected for histopathological and electron microscopical examinations. Results: The number of viable pyramidal cells within CA1 hippocampal region in sham control and NSO treated groups was significantly higher than that of untreated 2VO group, while the difference was not significant when comparing the viable pyramidal cells number of sham control with NSO treated groups. Furthermore, 2VO group showed marked intracellular ultrastructural distortions that were less pronounced in NSO treated group. Conclusion: NSO displayed a robust potential to protect hippocampal pyramidal cells from CBH induced neurodegeneration putting forward its prospective neuroprotective activity against age related cognitive decline of Alzheimer’s disease and vascular dementia.
    Matched MeSH terms: Pyramidal Cells
  2. spa diversity of methicillin-resistant and -susceptible Staphylococcus aureus in clinical strains from Malaysia: a high prevalence of invasive European spa-type t032
    Jones SU, Chua KH, Chew CH, Yeo CC, Abdullah FH, Othman N, et al.
    PeerJ, 2021;9:e11195.
    PMID: 33889447 DOI: 10.7717/peerj.11195
    Background: Staphylococcus aureus is one of the important pathogens causing nosocomial infection. spa typing allows identification of S. aureus clones in hospital isolates and is useful for epidemiological studies and nosocomial infection control. This study aims to investigate the spa types in Malaysian S. aureus isolates obtained from various clinical specimens.

    Method: A total of 89 methicillin-resistant S. aureus (MRSA) [pus (n = 55), blood (n = 27), respiratory (n = 5), eye (n = 2)] isolates and 109 methicillin-susceptible S. aureus (MSSA) [pus (n = 79), blood (n = 24), respiratory (n = 3), eye (n = 2) and urine (n = 1)] isolates were subjected to spa typing with sequences analysed using BioNumerics version 7.

    Results: The spa sequence was successfully amplified from 77.8% of the strains (154/198) and 47 known spa types were detected. The distribution of known spa types in MRSA (36.2%, 17/47) was less diverse than in MSSA (70.2%, 33/47). The most predominant spa types were t032 (50%) in MRSA, and t127 (19%) and t091 (16.7%) in MSSA, respectively. spa type t091 in MSSA was significantly associated with skin and soft tissue infections (p = 0.0199).

    Conclusion: The previously uncommon spa type t032 was detected in the Malaysian MRSA strains, which also corresponded to the most common spa type in Europe and Australia, and has replaced the dominant spa type t037 which was reported in Malaysia in 2010.

    Matched MeSH terms: Clone Cells
  3. Cytotoxicity and nitric oxide inhibitory activities of Xanthones isolated from Calophyllum hosei Ridl
    Daud S, Karunakaran T, Santhanam R, Nagaratnam SR, Jong VYM, Ee GCL
    Nat Prod Res, 2020 Sep 09.
    PMID: 32901512 DOI: 10.1080/14786419.2020.1819273
    Previous studies on Calophyllum species have shown the existence of a wide variety of bioactive xanthones and coumarins. Phytochemical investigations carried out on the plant, Calophyllum hosei led to the isolation of eleven known xanthones, ananixanthone (1), 9-hydroxycalabaxanthone (2), dombakinaxanthone (3), thwaitesixanthone (4), caloxanthone B (5), trapezifolixanthone (6), β-mangostin (7), osajaxanthone (8), caloxanthone A (9), calozeyloxanthone (10) and rubraxanthone (11). The structures of these compounds were identified and elucidated using spectroscopic techniques such as NMR and MS. The cytotoxicity and nitric oxide production inhibitory activities of selected xanthones as well as the extracts were tested against HL-60 cells and RAW 264.7 murine macrophages, respectively. Among all tested compounds, β-mangostin exhibited appreciable cytotoxicity against HL-60 cells with the IC50 value of 7.16 ± 0.70 µg/mL and rubraxanthone exhibited significant nitric oxide inhibitory activity against LPS induced RAW 264.7 murine macrophages with the IC50 value of 6.45 ± 0.15 µg/mL.
    Matched MeSH terms: HL-60 Cells
  4. Fulltext Reflux of Endoplasmic Reticulum proteins to the cytosol inactivates tumor suppressors
    Sicari D, Centonze FG, Pineau R, Le Reste PJ, Negroni L, Chat S, et al.
    EMBO Rep, 2021 May 05;22(5):e51412.
    PMID: 33710763 DOI: 10.15252/embr.202051412
    In the past decades, many studies reported the presence of endoplasmic reticulum (ER)-resident proteins in the cytosol. However, the mechanisms by which these proteins relocate and whether they exert cytosolic functions remain unknown. We find that a subset of ER luminal proteins accumulates in the cytosol of glioblastoma cells isolated from mouse and human tumors. In cultured cells, ER protein reflux to the cytosol occurs upon ER proteostasis perturbation. Using the ER luminal protein anterior gradient 2 (AGR2) as a proof of concept, we tested whether the refluxed proteins gain new functions in the cytosol. We find that refluxed, cytosolic AGR2 binds and inhibits the tumor suppressor p53. These data suggest that ER reflux constitutes an ER surveillance mechanism to relieve the ER from its contents upon stress, providing a selective advantage to tumor cells through gain-of-cytosolic functions-a phenomenon we name ER to Cytosol Signaling (ERCYS).
    Matched MeSH terms: Cells, Cultured
  5. Fulltext Growth kinetics of citrus suhuiensis cell suspension
    Noor Illi Mohamad Puad, Muhammad Alif Sarji, Nur Alia M. Fathil, Muhammad Yusuf Abduh
    Biological and Natural Resources Engineering Journal, 2018;1(1):1-13.
    MyJurnal
    Citrus is one of the major commodities in many countries including Malaysia.
    However, production of citrus including Citrus suhuiensis (C. suhuiensis) is declining due to
    diseases and inability to withstand low temperatures. Plant cultures such as cell suspension have the
    potential in propagating disease-free and healthy Citrus fruits with value-added characteristics.
    However, studies related to C. suhuiensis is still scarce. Therefore, the growth kinetics of C.
    suhuiensis cell suspension culture was studied. Friable callus of C. suhuiensis which was induced
    from seeds was inoculated into MS medium with 30 g/L sucrose, 0.5 g/L malt extract and 2.0 mg/L
    2, 4-D for the cell suspension initiation. Several batch experiments using a few types of sugars
    (sucrose, glucose and fructose) were carried out. The cell dry weight (CDW) of C. suhuiensis was
    recorded for 30 days of culture period and residual sugars in the medium were analyzed using
    HPLC. Cells grown in 30 g/L sucrose achieved the highest CDW (9.559 g/L) with µmax equals to
    0.00512/h, compared to glucose and fructose. In addition, sucrose is the preferred carbon source
    with the highest uptake rate (0.213 g/L·h). Cells completely hydrolyzed sucrose into glucose and
    fructose after 5 days of inoculation. All sugars were completely utilized by C. suhuiensis cells after
    25 days. The kinetic growth parameters determined from batch experiments were then used for
    model simulation and verification in MATHCAD 15. After adjustments and refinement to the
    selected kinetic parameters, the model has fairly described and predicted the growth and sugars
    profile of C. suhuiensis cells. The proposed model can be used to predict sucrose hydrolysis, glucose
    and fructose formation from sucrose and their consumption by plant cells and also for larger scale
    of growth.
    Matched MeSH terms: Plant Cells
  6. A profile of TNFR2+ regulatory T cells and CD103+ dendritic cells in the peripheral blood of patients with asthma
    Azid NA, Ahmad S, Boer JC, Al-Hatamleh MAI, Mohammad N, Mohd Ashari NS, et al.
    Hum Immunol, 2020 08 06;81(10-11):634-643.
    PMID: 32771274 DOI: 10.1016/j.humimm.2020.07.006
    The interaction of tolerogenic CD103+ dendritic cells (DCs) with regulatory T (Tregs) cells modulates immune responses by inducing immune tolerance. Hence, we determined the proportion of these cells in the peripheral blood mononuclear cells (PBMC) of asthmatic patients. We observed lower trends of CD11b-CD103+ DCs and CD86 within CD11b-CD103+ DCs, while increased levels of Foxp3 expressing CD25+/-TNFR2+ cells in asthmatics. There was a positive correlation in the expression of Foxp3 within CD3+CD4+CD25+TNFR2+ Tregs and CD11b-CD103+ as well as the expression of CD86 within HLA-DR+CD11c+CD11b-CD103+ DCs. In conclusion, we suggest that the increased levels of Tregs in blood could continuously suppress the T helper 2 (Th2) cells activation in the circulation which is also supported by the increase of anti-inflammatory cytokines IL-10 and TNF. Overall, functional immunoregulation of the regulatory cells, particularly Tregs, exhibit immune suppression and induce immune tolerance linked with the immune activation by the antigen presenting cells (APC).
    Matched MeSH terms: Dendritic Cells
  7. Large-Scale Expansion of Human Mesenchymal Stem Cells
    Hassan MNFB, Yazid MD, Yunus MHM, Chowdhury SR, Lokanathan Y, Idrus RBH, et al.
    Stem Cells Int, 2020;2020:9529465.
    PMID: 32733574 DOI: 10.1155/2020/9529465
    Mesenchymal stem cells (MSCs) are multipotent stem cells with strong immunosuppressive property that renders them an attractive source of cells for cell therapy. MSCs have been studied in multiple clinical trials to treat liver diseases, peripheral nerve damage, graft-versus-host disease, autoimmune diseases, diabetes mellitus, and cardiovascular damage. Millions to hundred millions of MSCs are required per patient depending on the disease, route of administration, frequency of administration, and patient body weight. Multiple large-scale cell expansion strategies have been described in the literature to fetch the cell quantity required for the therapy. In this review, bioprocessing strategies for large-scale expansion of MSCs were systematically reviewed and discussed. The literature search in Medline and Scopus databases identified 26 articles that met the inclusion criteria and were included in this review. These articles described the large-scale expansion of 7 different sources of MSCs using 4 different bioprocessing strategies, i.e., bioreactor, spinner flask, roller bottle, and multilayered flask. The bioreactor, spinner flask, and multilayered flask were more commonly used to upscale the MSCs compared to the roller bottle. Generally, a higher expansion ratio was achieved with the bioreactor and multilayered flask. Importantly, regardless of the bioprocessing strategies, the expanded MSCs were able to maintain its phenotype and potency. In summary, the bioreactor, spinner flask, roller bottle, and multilayered flask can be used for large-scale expansion of MSCs without compromising the cell quality.
    Matched MeSH terms: Multipotent Stem Cells
  8. Hydralazine administration activates sympathetic preganglionic neurons whose activity mobilizes glucose and increases cardiovascular function
    Parker LM, Damanhuri HA, Fletcher SP, Goodchild AK
    Brain Res, 2015 Apr 16;1604:25-34.
    PMID: 25662772 DOI: 10.1016/j.brainres.2015.01.049
    Hypotensive drugs have been used to identify central neurons that mediate compensatory baroreceptor reflex responses. Such drugs also increase blood glucose. Our aim was to identify the neurochemical phenotypes of sympathetic preganglionic neurons (SPN) and adrenal chromaffin cells activated following hydralazine (HDZ; 10mg/kg) administration in rats, and utilize this and SPN target organ destination to ascribe their function as cardiovascular or glucose regulating. Blood glucose was measured and adrenal chromaffin cell activation was assessed using c-Fos immunoreactivity (-ir) and phosphorylation of tyrosine hydroxylase, respectively. The activation and neurochemical phenotype of SPN innervating the adrenal glands and celiac ganglia were determined using the retrograde tracer cholera toxin B subunit, in combination with in situ hybridization and immunohistochemistry. Blood glucose was elevated at multiple time points following HDZ administration but little evidence of chromaffin cell activation was seen suggesting non-adrenal mechanisms contribute to the sustained hyperglycemia. 16±0.1% of T4-T11 SPN contained c-Fos and of these: 24.3±1.4% projected to adrenal glands and 29±5.5% projected to celiac ganglia with the rest innervating other targets. 62.8±1.4% of SPN innervating adrenal glands were activated and 29.9±3.3% expressed PPE mRNA whereas 53.2±8.6% of SPN innervating celiac ganglia were activated and 31.2±8.8% expressed PPE mRNA. CART-ir SPN innervating each target were also activated and did not co-express PPE mRNA. Neurochemical coding reveals that HDZ administration activates both PPE+SPN, whose activity increase glucose mobilization causing hyperglycemia, as well as CART+SPN whose activity drive vasomotor responses mediated by baroreceptor unloading to raise vascular tone and heart rate.
    Matched MeSH terms: Chromaffin Cells/drug effects; Chromaffin Cells/enzymology; Chromaffin Cells/metabolism
  9. Fulltext CD80 Insights as Therapeutic Target in the Current and Future Treatment Options of Frequent-Relapse Minimal Change Disease
    Teh YM, Lim SK, Jusoh N, Osman K, Mualif SA
    Biomed Res Int, 2021;2021:6671552.
    PMID: 33506028 DOI: 10.1155/2021/6671552
    Minimal change disease (MCD) is the most common cause of idiopathic nephrotic syndrome in children, and it is well known for its multifactorial causes which are the manifestation of the disease. Proteinuria is an early consequence of podocyte injury and a typical sign of kidney disease. Steroid-sensitive patients react well with glucocorticoids, but there is a high chance of multiple relapses. CD80, also known as B7-1, is generally expressed on antigen-presenting cells (APCs) in steroid-sensitive MCD patients. Various glomerular disease models associated with proteinuria demonstrated that the detection of CD80 with the increase of urinary CD80 was strongly associated closely with frequent-relapse MCD patients. The role of CD80 in MCD became controversial because one contradicts finding. This review covers the treatment alternatives for MCD with the insight of CD80 as a potential therapeutic target. The promising effectiveness of CD20 (rituximab) antibody and CD80 inhibitor (abatacept) encourages further investigation of CD80 as a therapeutic target in frequent-relapse MCD patients. Therapeutic-based antibody towards CD80 (galiximab) had never been investigated in MCD or any kidney-related disease; hence, the role of CD80 is still undetermined. A new therapeutic approach towards MCD is essential to provide broader effective treatment options besides the general immunosuppressive agents with gruesome adverse effects.
    Matched MeSH terms: Antigen-Presenting Cells
  10. Asperginols A and B, Diterpene Pyrones, from an Aspergillus sp. and the Structure Revision of Previously Reported Analogues
    Al-Khdhairawi AAQ, Low YY, Manshoor N, Arya A, Jelecki M, Alshawsh MA, et al.
    J Nat Prod, 2020 12 24;83(12):3564-3570.
    PMID: 33305943 DOI: 10.1021/acs.jnatprod.0c00618
    Two new diterpene pyrones, asperginols A (1) and B (2), and four known analogues (3-6) were isolated from the endophytic fungus Aspergillus sp. HAB10R12. The structures and absolute configurations of these compounds were elucidated based on the analysis of their NMR, MS, and X-ray diffraction data. The revision of the absolute configurations at C-10, C-11, and C-14 of the known diterpene pyrones (3-6) and the determination of the configuration at the polyene side chain for compounds (4-6) were made using chemical methods and vibrational circular dichroism analysis. This group of diterpene pyrone compounds showed unique structural features including a 7/6/6 tricyclic diterpene moiety with an unusual trans-syn-trans stereochemical arrangement. Compound 6 showed moderate activity against the HT-29 colon cancer cell line.
    Matched MeSH terms: HT29 Cells
  11. Fulltext Computational approaches: discovery of GTPase HRas as prospective drug target for 1,3-diazine scaffolds
    Kumar S, Sharma D, Narasimhan B, Ramasamy K, Shah SAA, Lim SM, et al.
    BMC Chem, 2019 Dec;13(1):96.
    PMID: 31355369 DOI: 10.1186/s13065-019-0613-8
    Heterocyclic 1,3-diazine nucleus is a valuable pharmacophore in the field of medicinal chemistry and exhibit a wide spectrum of biological activities. PharmMapper, a robust online tool used for establishing the target proteins based on reverse pharmacophore mapping. PharmMapper study is carried out to explore the pharmacological activity of 1,3-diazine derivatives using reverse docking program. PharmMapper, an open web server was used to recognize for all the feasible target proteins for the developed compounds through reverse pharmacophore mapping. The results were analyzed via molecular docking with maestro v11.5 (Schrodinger 2018-1) using GTPase HRas as possible target. The molecular docking studies displayed the binding behavior of 1,3-diazine within GTP binding pocket. From the docking study compounds s3 and s14 showed better docked score with anticancer potency against cancer cell line (HCT116). Hence, the GTPase HRas may be the possible target of 1,3-diazine derivatives for their anticancer activity where the retrieved information may be quite useful for developing rational drug designing. Furthermore the selected 1,3-diazine compounds were evaluated for their in vitro anticancer activity against murine macrophages cell line. 1,3-Diazine compounds exhibited good selectivity of the compounds towards the human colorectal carcinoma cell line instead of the murine macrophages. The toxicity study of the most active compounds was also performed on non cancerous HEK-293 cell line.
    Matched MeSH terms: HEK293 Cells
  12. Fulltext Agarwood branch ethanolic extract: optimal extraction process conditions and cytotoxic effects
    Phirdaous Abbas, Yumi Zuhanis Has-Yun Hashim, Hamzah Mohd Salleh, Saripah Salbiah Syed Abdul Azzizz
    Biological and Natural Resources Engineering Journal, 2020;3(2):7-26.
    MyJurnal
    Uninfected agarwood branch is readily available as raw material in agarwood plantation as new practices of agarwood plantation scheme were opted as substitute to the endangered wild type agarwood. The uninfected branch can be easily obtained during pruning process (one of plantation’s common maintenance procedure), throughout the years before inoculation stage. This current study aimed to investigate the optimal extraction process conditions of agarwood branch using ethanol as solvent system for maximal yield, and assess its cytotoxic effects towards MCF-7 breast cancer cells. Uninfected branch of Aquilaria subintegra was subjected to One Factor at a Time (OFAT) and Response Surface Methodology (RSM)-guided ethanolic extraction to achieve maximal yield. The extract was then subjected to cytotoxicity, cell attachment and cell viability assays, respectively. Optimization Run 2 (temperature 45 °C, solid-liquid ratio of 1:30, 16 hours maceration) gave the highest agarwood branch ethanolic extract (ABEE) yield of 44.70 ± 18.9 mg/g dried material (DM). Meanwhile Run 7 (temperature 45 °C, solid-liquid ratio of 1:10, 16 hours of maceration) gave the lowest yield (19.34 ± 14.1 mg/g DM). However, while maintaining the 16 hour-maceration, the model predicted a slightly lower yield of 30.232 ± 0.266 mg/g DM of ABEE with process conditions of 45 °C and solid-liquid ratio of 1:19 when the desirable parameters were factored in namely using (ⅰ) the most suitable temperature (that does not risk the bioactivities of the extract), and (ⅱ) an economical volume of solvent. Crude ABEE obtained from the optimal process conditions resulted in cytotoxicity effects on MCF-7 breast cancer cells with IC50 estimate of 3.645 ± 0.099 μg/mL. The extract also affected MCF-7 cell attachment and viability with altered morphology. More work to elucidate the mechanism of actions of the extract are warranted; which could further lead to development of breast cancer natural product-based therapeutics.
    Matched MeSH terms: MCF-7 Cells
  13. Gene expression changes in human bronchial epithelial cells (BEAS-2B) and human pulmonary alveolar epithelial cells (HPAEpiC) after interaction with Cladosporium sphaerospermum
    Lo SG, Wong SF, Mak JW, Choo KK, Ng KP
    Med Mycol, 2020 Apr 01;58(3):333-340.
    PMID: 31309220 DOI: 10.1093/mmy/myz061
    Cladosporium is one of the most abundant spore. Fungi of this genus can cause respiratory allergy and intrabronchial lesion. We studied the differential expression of host genes after the interaction of Cladosporium sphaerospermum conidia with Human Bronchial Epithelial Cells (BEAS-2B) and Human Pulmonary Alveolar Epithelial Cells (HPAEpiC). C. sphaerospermum conidia were harvested and co-cultured with BEAS-2B cells or HPAEpiC cells for 48 hours respectively. This culture duration was chosen as it was associated with high germination rate. RNA was extracted from two biological replicates per treatment. RNA of BEAS-2B cells was used to assess changes in gene expression using AffymetrixGeneChip® Human Transcriptome Array 2.0. After co-culture with Cladosporium spores, 68 individual genes were found differentially expressed (P ≤ 0.05) and up-regulated ≥ 1.5 folds while 75 genes were found differentially expressed at ≤ -1.5 folds compared with controls. Reverse transcription and qPCR were performed on the RNA collected from both BEAS-2B cells and HPAEpiC cells to validate the microarray results with 7 genes. Based on the findings, infected pulmonary epithelial cells exhibited an increase in cell death-related genes and genes associated with innate immunity.
    Matched MeSH terms: Epithelial Cells
  14. A highly sensitive fluorescent viscosity sensor
    Yusop RM, Unciti-Broceta A, Bradley M
    Bioorg Med Chem Lett, 2012 Sep 15;22(18):5780-3.
    PMID: 22901897 DOI: 10.1016/j.bmcl.2012.07.101
    Variation at the 3' position of fluorescein via Suzuki-Miyaura cross-coupling with aryl and heteroaryl moieties gave a family of anthofluoresceins whose spectroscopic properties were studied. The 1-methylindole derivative gave the highest quantum yield and was observed to behave as a molecular rotor, displaying marked variations in fluorescent intensities with viscosity and offering possible application in cellular sensing and fluorescent polarisation assays.
    Matched MeSH terms: HeLa Cells
  15. Phytochemistry, cytotoxicity and antiviral activity of Catharanthus roseus
    Wahab NZA, Azizul A, Ibrahim N
    Iran J Microbiol, 2020 Oct;12(5):460-465.
    PMID: 33604002 DOI: 10.18502/ijm.v12i5.4608
    Background and Objectives: Catharanthus roseus is generally used to treat many diseases in folklore remedies. The present study is aimed at determining phytochemical constituents, cytotoxicity and antiviral activities for crude extract of the plant.

    Materials and Methods: The whole plant of C. roseus was extracted using methanol extraction method. Phytochemical qualitative screening was carried out for C. roseus extract according to standard procedures used to test for the presence of alkaloid, saponin, terpenoid and steroid. Cytotoxicity was assessed using 3-(4,5-dimethylthiazol-2,5-diphenyltetrazolium bromide (MTT) assay. Plaque reduction assays were carried out to evaluate the antiviral activity of C. roseus extract against herpes simplex virus type 1 (HSV-1). These include post-treatment, pre-treatment and virucidal assays.

    Results: C. roseus extract contain secondary metabolites such as alkaloid, saponin and terpenoid but does not contain steroid. Cytotoxicity screening against Vero cells using MTT assay showed that the CC50 values for crude extract of C. roseus was 0.5 mg/mL. The extract prepared from C. roseus possesses phytochemical compound that was non-cytotoxic to the cell with potential antiviral activity. Plaque reduction assays against herpes simplex virus type 1 (HSV-1) showed that the selective indices (SI = CC50 / EC50) of C. roseus extract in post-treatment, pre-treatment and virucidal assays were 36, 20 and 4.7 respectively. The results revealed that the extract prepared from C. roseus possesses phytochemical compound that was non-cytotoxic to the cell with potential antiviral activity.

    Conclusion: This study showed that C. roseus extract has promising potential to be explored as anti-HSV-1 agent regardless of the mode of treatment.

    Matched MeSH terms: Vero Cells
  16. Comparative proteomics of Mesembryanthemum crystallinum guard cells and mesophyll cells in transition from C3 to CAM
    Guan Q, Kong W, Zhu D, Zhu W, Dufresne C, Tian J, et al.
    J Proteomics, 2021 01 16;231:104019.
    PMID: 33075550 DOI: 10.1016/j.jprot.2020.104019
    Salinity can induce Mesembryanthemum crystallinum to shift its photosynthesis from C3 to crassulacean acid metabolism (CAM), leading to enhanced plant water use efficiency. Studying how M. crystallinum changes its carbon fixation pathways is important for potential translation into crops and enhancing crop resilience. In this study, we examined proteomic changes in guard cells and mesophyll cells in the course of the C3 to CAM transition. We collected enriched guard cells and mesophyll cells during a short period of transition. A total of 1153 proteins were identified and quantified in the two cell-types. During the transition, proteins in the guard cells and mesophyll cells exhibited differential changes. For example, we observed nocturnal carbon fixation in mesophyll cells and proteins involved in cell growth in the two cell-types. Proteins involved in osmotic adjustment, ion transport, energy metabolism and light response may play important roles in the C3 to CAM transition. Real-time PCR experiments were conducted to determine potential correlations between transcript and protein levels. These results have highlighted potential molecular mechanisms underlying the C3 to CAM transition of guard cells and mesophyll cells of the important facultative CAM plant. BIOLOGICAL SIGNIFICANCE: Fresh water resource for agricultural food production is a global challenge. Nature has evolved crassulacean acid metabolism (CAM) plants with enhanced water use efficiency. Using single cell-type proteomics, this study revealed molecular changes taking place in guard cells and mesophyll cells during the shift of ice plant photosynthesis from C3 to CAM. The results have provided important insights into the CAM transition and may facilitate effort toward enhancing crop resilience for global food security.
    Matched MeSH terms: Mesophyll Cells
  17. Fulltext Capilliposide B blocks VEGF-induced angiogenesis in vitro in primary human retinal microvascular endothelial cells
    Han H, Yang Y, Wu Z, Liu B, Dong L, Deng H, et al.
    Biomed Pharmacother, 2021 Jan;133:110999.
    PMID: 33227710 DOI: 10.1016/j.biopha.2020.110999
    Abnormal angiogenesis is associated with intraocular diseases such as proliferative diabetic retinopathy and neovascular age-related macular degeneration, and current therapies for these eye diseases are not satisfactory. The purpose of this study was to determine whether capilliposide B (CPS-B), a novel oleanane triterpenoid saponin derived from Lysimachia capillipes Hemsl, can inhibit vascular endothelial growth factor (VEGF)-induced angiogenesis signaling events and cellular responses in primary human retinal microvascular endothelial cells (HRECs). Our study revealed that the capilliposide B IC50 for HRECs was 8.5 μM at 72 h and that 1 μM capilliposide B specifically inhibited VEGF-induced activation of VEGFR2 and its downstream signaling enzymes Akt and Erk. In addition, we discovered that this chemical effectively blocked VEGF-stimulated proliferation, migration and tube formation of the HRECs, suggesting that capilliposide B is a promising prophylactic for angiogenesis-associated diseases such as proliferative diabetic retinopathy.
    Matched MeSH terms: Cells, Cultured; Endothelial Cells/drug effects*; Endothelial Cells/metabolism
  18. Caspase-dependent and -independent mechanisms in apoptosis induced by hydroquinone and catechol metabolites of remoxipride in HL-60 cells
    Inayat-Hussain SH, McGuinness SM, Johansson R, Lundstrom J, Ross D
    Chem Biol Interact, 2000 Aug 15;128(1):51-63.
    PMID: 10996300
    The hydroquinone and catechol like metabolites, NCQ344 and NCQ436 respectively, of the antipsychotic remoxipride have recently been demonstrated to induce apoptosis in myeloperoxidase (MPO)-rich human bone marrow progenitor and HL-60 cells [S.M. McGuinness, R. Johansson, J. Lundstrom, D. Ross, Induction of apoptosis by remoxipride metabolites in HL-60 and CD34+/CD19- human bone marrow progenitor cells: potential relevance to remoxipride-induced aplastic anemia, Chem. Biol. Interact. 121 (1999) 253-265]. In the present study, we determined the molecular mechanisms of apoptosis induced by these remoxipride metabolites in HL-60 cells. Our results show that apoptosis was accompanied by phosphatidylserine (PS) exposure, activation of caspases-9, -3, -7 and DNA cleavage. In HL-60 cells treated with the hydroquinone NCQ344 and catechol NCQ436, the general caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp. fluoromethyl ketone (Z-VAD.FMK) blocked DNA cleavage and activation of caspases-9, -3/-7. In addition, PS exposure was significantly but not completely inhibited by Z-VAD.FMK. These results demonstrate that although Z-VAD.FMK inhibitable caspases are necessary for maximal apoptosis induced by NCQ344 and NCQ436, additional caspase-independent processes may orchestrate changes leading to PS exposure during apoptosis induced by the remoxipride polyphenolic metabolites.
    Matched MeSH terms: HL-60 Cells/cytology; HL-60 Cells/drug effects; HL-60 Cells/enzymology
  19. Comparison of different methods for the isolation of mesenchymal stem cells from human umbilical cord Wharton's jelly
    Salehinejad P, Alitheen NB, Ali AM, Omar AR, Mohit M, Janzamin E, et al.
    In Vitro Cell Dev Biol Anim, 2012 Feb;48(2):75-83.
    PMID: 22274909 DOI: 10.1007/s11626-011-9480-x
    Several techniques have been devised for the dissociation of tissues for primary culture. These techniques can affect the quantity and quality of the isolated cells. The aim of our study was to develop the most appropriate method for the isolation of human umbilical cord-derived mesenchymal (hUCM) cells. In the present study, we compared four methods for the isolation of hUCM cells: three enzymatic methods; collagenase/hyaluronidase/trypsin (CHT), collagenase/trypsin (CT) and trypsin (Trp), and an explant culture (Exp) method. The trypan blue dye exclusion test, the water-soluble tetrazolium salt-1 (WST-1) assay, flow cytometry, alkaline phosphatase activity and histochemical staining were used to evaluate the results of the different methods. The hUCM cells were successfully isolated by all methods but the isolation method used profoundly altered the cell number and proliferation capacity of the isolated cells. The cells were successfully differentiated into adipogenic and osteogenic lineages and alkaline phosphatase activity was detected in the hUCM cell colonies of all groups. Flow cytometry analysis revealed that CD44, CD73, CD90 and CD105 were expressed in all groups, while CD34 and CD45 were not expressed. The expression of C-kit in the enzymatic groups was higher than in the explant group, while the expression of Oct-4 was higher in the CT group compared to the other groups. We concluded that the collagenase/trypsin method of cell isolation yields a higher cell density than the others. These cells expressed a higher rate of pluripotent cell markers such as C-kit and Oct-4, while the explant method of cell isolation resulted in a higher cell proliferation rate and activity compared to the other methods.
    Matched MeSH terms: Cells, Cultured; Mesenchymal Stromal Cells/cytology*; Mesenchymal Stromal Cells/metabolism
  20. Fulltext Antifungal efficacy of crude aqueous weed extracts against pathogen of cocoa black pod rot
    Nor Amerulah Nor Mohamad, Suhaida Salleh, Hamzah Abdul Aziz
    Borneo Akademika, 2019;3(2):12-22.
    MyJurnal
    Black pod rot is the most economically important disease of cocoa in Malaysia which is
    mainly caused by a highly polyphagous Phytophthora species, called Phytophthora palmivora.
    The fungus could attack all parts of the cocoa plant organs and caused various diseases at
    any growth stage from seedling until the mature stages, especially during raining season. The
    application of synthetic fungicides has been widely recommended to manage the disease but
    their repeated use had led to other problems such as environmental, human health and
    development of fungicide resistance issues. This study isolated and identified Phytopththora
    isolate from a cocoa pod sample based on micro-morphological characters. Besides, the
    present investigation was undertaken to screen for the antifungal potency of different weed
    extracts against the Phytophthora pathogen using poisoned food technique. The fungal isolate
    was successfully recovered from pod tissues of clone PBC123 on 20% tomato juice agar
    culture (20T). Only one out of ten weed extracts tested showed a significant in vitro inhibitory
    effect towards mycelial growth of Phytophthora isolate, which was aqueous crude leaf extract
    of Solanum torvum (42.68%). This study indicated that the potential of weed extracts in the
    management of Phytophthora diseases, and may offer more natural, effective and economical
    control methods.
    Matched MeSH terms: Clone Cells
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