Displaying publications 121 - 140 of 366 in total

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  1. Antibiofilm activity and mode of action of DMSO alone and its combination with afatinib against Gram-negative pathogens
    Yahya MFZR, Alias Z, Karsani SA
    Folia Microbiol (Praha), 2018 Jan;63(1):23-30.
    PMID: 28540585 DOI: 10.1007/s12223-017-0532-9
    Biofilms are complex microbial communities that tend to attach to either biotic or abiotic surface. Enclosed in a self-produced extracellular polymeric substance (EPS) matrix, the biofilms often cause persistent infections. The objective of this study was to investigate the antibiofilm activity of dimethyl sulfoxide (DMSO) and afatinib against Gram-negative pathogens. Test microorganisms used in this study were Escherichia coli ATCC 1299, Pseudomonas aeruginosa ATCC 10145, and Salmonella typhimurium ATCC 14028. Biofilms were developed in 96-well microplate at 37°C for 24 h. Following removal of non-adherent cells, analysis of biofilm viability, biofilm biomass, and extracellular polymeric substances (EPS) matrix were performed using resazurin assay, crystal violet assay, and attenuated total reflectance fourier transform infrared (ATR-FTIR) spectroscopy, respectively. Bradford protein assay was conducted to determine the total amount of EPS proteins. The results demonstrated that both 32% DMSO alone and its combination with 3.2 μg/mL afatinib were effective in killing biofilm cells and reducing biofilm biomass. IR spectral variations of EPS matrix of biofilms in the range between 1700 and 900 cm-1 were also observed. Reduction in EPS proteins verified the chemical modifications of EPS matrix. In conclusion, 32% DMSO alone and its combination with 3.2 μg/mL afatinib showed remarkable antibiofilm activities against Gram-negative pathogens. It was suggested that the biofilm inhibition was mediated by the chemical modification of EPS matrix.
    Matched MeSH terms: Pseudomonas aeruginosa/drug effects*; Pseudomonas aeruginosa/physiology
  2. Fulltext Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition
    Yip CH, Mahalingam S, Wan KL, Nathan S
    PLoS One, 2021;16(6):e0253445.
    PMID: 34161391 DOI: 10.1371/journal.pone.0253445
    Prodigiosin, a red linear tripyrrole pigment, has long been recognised for its antimicrobial property. However, the physiological contribution of prodigiosin to the survival of its producing hosts still remains undefined. Hence, the aim of this study was to investigate the biological role of prodigiosin from Serratia marcescens, particularly in microbial competition through its antimicrobial activity, towards the growth and secreted virulence factors of four clinical pathogenic bacteria (methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecalis, Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa) as well as Staphylococcus aureus and Escherichia coli. Prodigiosin was first extracted from S. marcescens and its purity confirmed by absorption spectrum, high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrophotometry (LC-MS/MS). The extracted prodigiosin was antagonistic towards all the tested bacteria. A disc-diffusion assay showed that prodigiosin is more selective towards Gram-positive bacteria and inhibited the growth of MRSA, S. aureus and E. faecalis and Gram-negative E. coli. A minimum inhibitory concentration of 10 μg/μL of prodigiosin was required to inhibit the growth of S. aureus, E. coli and E. faecalis whereas > 10 μg/μL was required to inhibit MRSA growth. We further assessed the effect of prodigiosin towards bacterial virulence factors such as haemolysin and production of protease as well as on biofilm formation. Prodigiosin did not inhibit haemolysis activity of clinically associated bacteria but was able to reduce protease activity for MRSA, E. coli and E. faecalis as well as decrease E. faecalis, Salmonella Typhimurium and E. coli biofilm formation. Results of this study show that in addition to its role in inhibiting bacterial growth, prodigiosin also inhibits the bacterial virulence factor protease production and biofilm formation, two strategies employed by bacteria in response to microbial competition. As clinical pathogens were more resistant to prodigiosin, we propose that prodigiosin is physiologically important for S. marcescens to compete against other bacteria in its natural soil and surface water environments.
    Matched MeSH terms: Pseudomonas aeruginosa/drug effects; Pseudomonas aeruginosa/growth & development
  3. Fulltext Phenotypic detection of metallo-β-lactamase in imipenem-resistant Pseudomonas aeruginosa
    Khosravi Y, Loke MF, Chua EG, Tay ST, Vadivelu J
    ScientificWorldJournal, 2012;2012:654939.
    PMID: 22792048 DOI: 10.1100/2012/654939
    Carbapenems are the primary choice of treatment for severe Pseudomonas aeruginosa infection. However, the emergence of carbapenem resistance due to the production of metallo-β-lactamases (MBLs) is of global concern. In this study, 90 imipenem- (IPM- or IP-) resistant P. aeruginosa (IRPA) isolates, including 32 previously tested positive and genotyped for MBL genes by PCR, were subjected to double-disk synergy test (DDST), combined disk test (CDT), and imipenem/imipenem-inhibitor (IP/IPI) E-test to evaluate their MBLs detection capability. All three methods were shown to have a sensitivity of 100%. However, DDST was the most specific of the three (96.6%), followed by IP/IPI E-test interpreted based on the single criteria of IP/IPI ≥8 as positive (62.1%), and CDT was the least specific (43.1%). Based on the data from this evaluation, we propose that only IRPA with IP MIC >16 μg/mL and IP/IPI ≥8 by IP/IPI E-test should be taken as positive for MBL activity. With the new dual interpretation criteria, the MBL IP/IPI E-test was shown to achieve 100% sensitivity as well as specificity for the IRPA in this study. Therefore, the IP/IPI E-test is a viable alternative phenotypic assay to detect MBL production in IRPA in our population in circumstances where PCR detection is not a feasible option.
    Matched MeSH terms: Pseudomonas aeruginosa/drug effects*; Pseudomonas aeruginosa/enzymology*
  4. Microbial biodiversity in a Malaysian oil field and a systematic comparison with oil reservoirs worldwide
    Li D, Midgley DJ, Ross JP, Oytam Y, Abell GC, Volk H, et al.
    Arch Microbiol, 2012 Jun;194(6):513-23.
    PMID: 22245906 DOI: 10.1007/s00203-012-0788-z
    Microbial diversity within formation water and oil from two compartments in Bokor oil reservoir from a Malaysian petroleum oil field was examined. A total of 1,056 16S rRNA gene clones were screened from each location by amplified ribosomal DNA restriction analysis. All samples were dominated by clones affiliated with Marinobacter, some novel Deferribacteraceae genera and various clones allied to the Methanococci. In addition, either Marinobacterium- or Pseudomonas-like operational taxonomic units were detected from either compartment. A systematic comparison with the existing pertinent studies was undertaken by analysing the microbial amplicons detected and the PCR primers used. The analyses demonstrated that bacterial communities were site specific, while Archaea co-occurred more frequently. Amplicons related to Marinobacter, Marinobacterium and Pseudomonas were detected in a number of the studies examined, suggesting they may be ubiquitous members in oil reservoirs. Further analysis of primers used in those studies suggested that most primer pairs had fairly broad but low matches across the bacterial and archaeal domains, while a minority had selective matches to certain taxa or low matches to all the microbial taxa tested. Thus, it indicated that primers may play an important role in determining which taxa would be detected.
    Matched MeSH terms: Pseudomonas/classification*; Pseudomonas/genetics
  5. Antibacterial activity and morphological changes of Pseudomonas aeruginosa cells after exposure to Vernonia cinerea extract
    Latha LY, Darah I, Kassim MJ, Sasidharan S
    Ultrastruct Pathol, 2010 Aug;34(4):219-25.
    PMID: 20594042 DOI: 10.3109/01913121003651513
    The antibacterial activity of Vernonia cinerea (L.) extract was investigated using the broth dilution method. The extract showed a favorable antimicrobial activity against Pseudomonas aeruginosa with a minimum inhibition concentration (MIC) value of 3.13 mg/mL. V. cinerea extract at (1/2), 1, or 2 times the MIC significantly inhibited bacterial growth with a noticeable drop in optical density (OD) of the bacterial culture, thus confirming the antibacterial activity of the extract on P. aeruginosa. Imaging using scanning (SEM) and transmission (TEM) electron microscopy was done to determine the major alterations in the microstructure of the extract-treated P. aeruginosa. The main abnormalities noted via SEM and TEM studies were the alteration in morphology of the bacterial cells. The main reason for this destruction was the severe alterations of the cell wall with the formation of holes, invaginations, and morphological disorganization caused by the extract. The authors conclude that the extract may be used as a candidate for the development of antimicrobial agents.
    Matched MeSH terms: Pseudomonas aeruginosa/drug effects*; Pseudomonas aeruginosa/ultrastructure
  6. Inhibitory effect of oxalic acid on bacterial spoilage of raw chilled chicken
    Anang DM, Rusul G, Radu S, Bakar J, Beuchat LR
    J Food Prot, 2006 Aug;69(8):1913-9.
    PMID: 16924917
    Oxalic acid was evaluated as a treatment for reducing populations of naturally occurring microorganisms on raw chicken. Raw chicken breasts were dipped in solutions of oxalic acid (0, 0.5, 1.0, 1.5, and 2.0%, wt/vol) for 10, 20, and 30 min, individually packed in oxygen-permeable polyethylene bags, and stored at 4 degrees C. Total plate counts of aerobic bacteria and populations of Pseudomonas spp. and Enterobacteriaceae on breasts were determined before treatment and after storage for 1, 3, 7, 10, and 14 days. The pH and Hunter L, a, and b values of the breast surface were measured. Total plate counts were ca. 1.5 and 4.0 log CFU/g higher on untreated chicken breasts after storage for 7 and 14 days, respectively, than on breasts treated with 0.5% oxalic acid, regardless of dip time. Differences in counts on chicken breasts treated with water and 1.0 to 2.0% of oxalic acid were greater. Populations of Pseudomonas spp. on chicken breasts treated with 0.5 to 2.0% oxalic acid and stored at 4 degrees C for 1 day were less than 2 log CFU/g (detection limit), compared with 5.14 log CFU/g on untreated breasts. Pseudomonas grew on chicken breasts treated with 0.5% oxalic acid to reach counts not exceeding 3.88 log CFU/g after storage for 14 days. Counts on untreated chicken exceeded 8.83 log CFU/g at 14 days. Treatment with oxalic acid caused similar reductions in Enterobacteriaceae counts. Kocuria rhizophila was the predominant bacterium isolated from treated chicken. Other common bacteria included Escherichia coli and Empedobacter brevis. Treatment with oxalic acid caused a slight darkening in color (decreased Hunter L value), retention of redness (increased Hunter a value), and increase in yellowness (increased Hunter b value). Oxalic acid has potential for use as a sanitizer to reduce populations of spoilage microorganisms naturally occurring on raw chicken, thereby extending chicken shelf life.
    Matched MeSH terms: Pseudomonas/drug effects*; Pseudomonas/growth & development
  7. Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K
    Rahman RN, Geok LP, Basri M, Salleh AB
    Bioresour Technol, 2005 Mar;96(4):429-36.
    PMID: 15491823
    The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 degrees C with 37 degrees C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0-10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media.
    Matched MeSH terms: Pseudomonas aeruginosa/classification*; Pseudomonas aeruginosa/enzymology*
  8. Fulltext Antibacterial activity of selected Malaysian honey
    Zainol MI, Mohd Yusoff K, Mohd Yusof MY
    BMC Complement Altern Med, 2013;13:129.
    PMID: 23758747 DOI: 10.1186/1472-6882-13-129
    Antibacterial activity of honey is mainly dependent on a combination of its peroxide activity and non-peroxide components. This study aims to investigate antibacterial activity of five varieties of Malaysian honey (three monofloral; acacia, gelam and pineapple, and two polyfloral; kelulut and tualang) against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa.
    Matched MeSH terms: Pseudomonas aeruginosa/drug effects; Pseudomonas aeruginosa/growth & development
  9. Fulltext The influence of agitation on oil substrate dispersion and oxygen transfer in Pseudomonas aeruginosa USM-AR2 fermentation producing rhamnolipid in a stirred tank bioreactor
    Nur Asshifa MN, Zambry NS, Salwa MS, Yahya ARM
    3 Biotech, 2017 Jul;7(3):189.
    PMID: 28664380 DOI: 10.1007/s13205-017-0828-0
    Water-immiscible substrate, diesel, was supplied as the main substrate in the fermentation of Pseudomonas aeruginosa USM-AR2 producing rhamnolipid biosurfactant, in a stirred tank bioreactor. In addition to the typical gas-aqueous system, this system includes gas-hydrocarbon-aqueous phases and the presence of surfactant (rhamnolipid) in the fermentation broth. The effect of diesel dispersion on volumetric oxygen transfer coefficient, k L a, and thus oxygen transfer, was evaluated at different agitations of 400, 500 and 600 rpm. The oxygen transfer in this oil-water-surfactant system was shown to be affected by different oil dispersion at those agitation rates. The highest diesel dispersion was obtained at 500 rpm or impeller tip speed of 1.31 m/s, compared to 400 and 600 rpm, which led to the highest k L a, growth and rhamnolipid production by P. aeruginosa USM-AR2. This showed the highest substrate mixing and homogenization at this agitation speed that led to the efficient substrate utilization by the cells. The oxygen uptake rate of P. aeruginosa USM-AR2 was 5.55 mmol/L/h, which showed that even the lowest k L a (48.21 h-1) and hence OTR (57.71 mmol/L/h) obtained at 400 rpm was sufficient to fulfill the oxygen demand of the cells. The effect of rhamnolipid concentration on k L a showed that k L a increased as rhamnolipid concentration increased to 0.6 g/L before reaching a plateau. This trend was similar for all agitation rates of 400, 500 and 600 rpm, which might be due to the increase in the resistance to oxygen transfer (k L decrease) and the increase in the specific interfacial area (a).
    Matched MeSH terms: Pseudomonas aeruginosa
  10. Fulltext Molecular detection of bacterial endosymbionts in acanthamoeba spp.: a preliminary study
    Faizah, M. H., Anisah, N., Yusof, S., Noraina, A. R., Adibah, M. R.
    Medicine & Health, 2017;12(2):286-292.
    MyJurnal
    Acanthamoeba spp. merupakan ameba hidup bebas yang biasa ditemui
    di persekitaran. Ia merupakan agen penyebab keratitis Acanthamoeba (AK)
    dan ensefalitis ameba bergranuloma (GAE). Ameba ini juga mampu menjadi
    perumah kepada pelbagai bakteria termasuklah yang bersifat patogenik seperti
    Mycobacterium, Legionella dan Staphylococcus aureus rintang metisilin (MRSA).
    Berdasarkan maklumat ini, satu kajian dijalankan untuk mengesan kehadiran tiga
    bakteria endosimbion berkepentingan perubatan di dalam Acanthamoeba spp. yang
    telah dipencilkan dari bolong penghawa dingin yang terdapat di wad and dewan
    bedah di Pusat Perubatan Universiti Kebangsaan Malaysia. Kehadiran bakteria
    endosimbion ini disaring menggunakan pasangan primer khusus bagi setiap genus
    menggunakan reaksi rantai polimerase (PCR) konvensional dan disahkan dengan
    analisis penjujukan. Dua puluh sembilan (80.56%) pencilan Acanthamoeba spp.
    didapati mengandungi bakteria endosimbion patogenik yang disasarkan dengan
    sekurang-kurangnya satu genus bakteria bagi setiap pencilan. Mycobacterium
    (82.76 %) adalah bakteria yang paling banyak dikesan, diikuti dengan Legionella sp.
    (65.52 %) dan Pseudomonas spp. (62.07 %). Tiada bakteria MRSA dikesan daripada
    mana-mana pencilan dalam kajian ini. Dua endosimbion Mycobacterium yang
    dikenalpasti telah dikelompokkan ke dalam strain Mycobacterium tuberculosis.
    Kami membuat kesimpulan bahawa, kebanyakan Acanthamoeba berpotensi untuk
    menjadi perumah bagi pelbagai bakteria patogenik, namun implikasi interaksi ini
    terhadap patogenisiti kedua-dua organisma masih kurang jelas dan memerlukan
    penyelidikan yang lebih lanjut.
    Matched MeSH terms: Pseudomonas
  11. Biofilm inhibiting activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms
    Yong YY, Dykes G, Lee SM, Choo WS
    J Appl Microbiol, 2019 Jan;126(1):68-78.
    PMID: 30153380 DOI: 10.1111/jam.14091
    AIMS: To investigate the biofilm inhibitory activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms.

    METHODS AND RESULTS: The pulp of red pitahaya and the leaves of red spinach were extracted using methanol followed by subfractionation to obtain betacyanin fraction. The anti-biofilm activity was examined using broth microdilution assay on polystyrene surfaces and expressed as minimum biofilm inhibitory concentration (MBIC). The betacyanin fraction from red spinach showed better anti-biofilm activity (MBIC: 0·313-1·25 mg ml-1 ) against five Staph. aureus strains while the betacyanin fraction from red pitahaya showed better anti-biofilm activity (MBIC: 0·313-0·625 mg ml-1 ) against four P. aeruginosa strains. Both betacyanin fraction significantly reduced hydrophobicity of Staph. aureus and P. aeruginosa strains. Numbers of Staph. aureus and P. aeruginosa attached to polystyrene were also reduced without affecting their cell viability.

    CONCLUSION: Betacyanins can act as anti-biofilm agents against the initial step of biofilm formation, particularly on a hydrophobic surface like polystyrene.

    SIGNIFICANCE AND IMPACT OF THE STUDY: This study is the first to investigate the use of betacyanin as a biofilm inhibitory agent. Betacyanin could potentially be used to reduce the risk of biofilm-associated infections.

    Matched MeSH terms: Pseudomonas aeruginosa/drug effects*; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/physiology
  12. Profiles of bacterial assemblages from microplastics of tropical coastal environments
    Curren E, Leong SCY
    Sci Total Environ, 2019 Mar 10;655:313-320.
    PMID: 30471599 DOI: 10.1016/j.scitotenv.2018.11.250
    Plastic waste is a global issue of an increasing concern in aquatic ecosystems. Microplastics form a large proportion of plastic pollution in marine environments. Although microplastics are prevalent, their distribution along the coasts of tropical regions is not well studied. Microplastic pieces (1-5 mm) were collected from two distinct regions along the coastlines of Singapore, from the northern coast in the Johor Strait and the southern coast in the Singapore Strait. Microplastics were present in concentrations ranging from 9.20-59.9 particles per kg of dry sand sediment. The majority of microplastics identified were foam particles (55%) and fragments (35%). Microplastics were significantly more abundant on heavily populated beaches compared to pristine beaches. High throughput sequencing was used to profile the communities of bacteria on the surfaces of microplastic particles. The structure of the microbial communities was primarily characterised by Proteobacteria and Bacteroidetes and were distinct across sites. Hydrocarbon-degrading genera such as Erythrobacter were dominant in areas with heavy shipping and pollution. Potential pathogenic genera such as Vibrio and Pseudomonas were also identified. This study highlights the diverse bacterial assemblages present on marine microplastic surfaces and the importance of understanding the bacterial plastisphere.
    Matched MeSH terms: Pseudomonas
  13. Fulltext The epidermal mucus extract of clarias batrachus inhibits gram negative bacteria growth
    Ahmad Zorin Sahalan, Engku Sharmila, Nazahiyah Sulaiman
    Jurnal Sains Kesihatan Malaysia, 2018;16(101):83-87.
    MyJurnal
    Recently, epidermal mucus of fish has been identified as a rich biological source of various bioactive substances, particularly antibacterial peptides. This study was conducted to identify the potential of epidermal mucosa of Clarias batrachus as a source of antibacterial material. Mucosal samples were collected from C. batrachus epidermis and extracted using an aqueous extraction method. Antibacterial activity of the crude epidermal mucus extract was determined by using spectrophotometric method. As a result there was antibacterial activity by the mucus extract against Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). The results of this study supports the role of mucus as a part of body immune system with antibacterial peptide as a component of innate body immune system. Hence, these findings can be used as one of the first steps towards the development of new class antibiotics.
    Matched MeSH terms: Pseudomonas aeruginosa
  14. Contact lens related pseudomonas corneal ulcer: a case report
    Sivaraj R, Verghese I, Lai YK, Parasakthi N
    Family Physician, 1994;6:18-20.
    Contact lenses provide an alternative to spectacles for many people. However, the hazard of bacterial corneal ulcers exists. This article describes a soft contact lens wearer who developed a bacterial corneal ulcer. Prompt diagnosis and treatment is essential for eradication of the infection and prevention of loss of vision.
    Matched MeSH terms: Pseudomonas
  15. Synthesis, characterization and antibacterial activity of mono-, di- and tri-tosylate of glycerol
    Yusrabbil Amiyati Yusof, Azhar Ariffin
    Sains Malaysiana, 2016;45:621-625.
    Glycerol is a valuable co-product from oleochemical industry such as from fatty acid and biodiesel production. By having three hydroxyl groups in its molecule, glycerol can undergo chemical modifications that lead to many possible applications. This paper reports the tosylation process of glycerol with para-toluenesulfonyl chloride (p-TsCl). Reaction of glycerol with p-TsCl in the presence of a base produced mono-, di- and tri-tosylate of glycerol even though the reaction was carried out at the mole ratio of 1.2:1.0 of glycerol to p-TsCl. The compounds were successfully isolated and characterized. Mono-, di- and tri-tosylate of glycerol exhibited inhibitory activity against Staphylococcus aureus (gram positive bacteria) and Pseudomonas aeruginosa (gram negative bacteria).
    Matched MeSH terms: Pseudomonas aeruginosa
  16. Fulltext Development of plant-based emulsion formulations to control bacterial leaf blight and sheath brown rot of rice
    Syed-Ab-Rahman SF, Carvalhais LC, Omar D
    Heliyon, 2020 Jan;6(1):e03151.
    PMID: 32042948 DOI: 10.1016/j.heliyon.2019.e03151
    Bacterial leaf blight (BLB) and sheath brown rot (SBR), caused by Xanthomonas oryzae pv. oryzae (Xoo) and Pseudomonas fuscovaginae, respectively, are bacterial diseases that lead to substantial yield losses in rice. Natural plant-based products represent a sustainable alternative to combat bacterial diseases due to their biodegradability and overall safety. However efficient ways of delivering them are crucial to their success. In an attempt to maximize the antibacterial properties of botanical bactericides for the control of these pathogens, this study evaluated the efficiency of different emulsion formulations of Piper sarmentosum extracts. The emulsion formulations were demonstrated to be effective in controlling BLB and SBR of rice in in vitro plate assays and in planta under glasshouse conditions. The observed in vitro inhibition of the bacterial pathogens and significant disease suppression in planta indicate that these plant extract formulations represent promising alternatives to be adopted in management strategies for controlling rice diseases.
    Matched MeSH terms: Pseudomonas
  17. Fulltext Factorial analysis of growth parameters for mesophilic bacterium, Pseudomonas putida (ATCC 49128)
    Ahmad, Mani Malam, Abd. Aziz Mohd Azoddein, Jami, Mohammed Saed
    Biological and Natural Resources Engineering Journal, 2018;1(1):50-57.
    MyJurnal
    : Studies on bacterial growth pattern from the conventional approach are defective due
    to their failure to explain the interactions or simply the complementary effects of the factors
    influencing the bacterial growth. In this study, the individual and collaborative effects of
    Pseudomonas putida growth variables were evaluated using a 2-level fractional factorial design of
    experiment (FFDOE). The growth of the organism was found to respond remarkably to different
    concentrations of nutrient media (carbon source) and the other independent variables. Factorial
    models were developed from the experimental design to study the individual and interactive effects
    of the studied parameters on the response. The studied parameters and their levels were as follows:
    nutrient concentration (4-16 g/L), acclimatization time (24-72 hrs), agitation (140-200 rpm), and
    temperature (30-40oC). These parameters were statistically validated using analysis of variance
    (ANOVA) and the results revealed that the model terms were statistically significant with an Fvalue of 415.17 at P temperature > nutrient concentration versus temperature >
    agitation > nutrient concentration versus agitation. Based on the R
    2
    and the adjusted R
    2
    values of
    >95%, the estimated variables showed a high degree of relationship between the observed and the
    predicted values; thus, the predictive ability of the models was suggested. It could, therefore, be
    concluded that nutrient concentration, temperature, and agitation can greatly influence the growth
    of P. putida within a specific range.
    Matched MeSH terms: Pseudomonas putida
  18. Draft Genome Sequence of Pseudomonas sp. LAB-08 Isolated from Trichloroethene-Contaminated Aquifer Soil
    Suzuki K, Aziz FA, Inuzuka Y, Tashiro Y, Futamata H
    Genome Announc, 2016;4(5).
    PMID: 27660772 DOI: 10.1128/genomeA.00948-16
    Pseudomonas sp. LAB-08 was isolated from a phenol-fed bioreactor constructed with contaminated aquifer soil as the inoculum. Strain LAB-08 utilized phenol as a sole carbon and energy source. Here, we report the genome sequence and annotation of Pseudomonas sp. LAB-08.
    Matched MeSH terms: Pseudomonas
  19. Fulltext Effect of Javanese turmeric (Curcuma xanthorrhiza Roxb.) extract on natural microflora of oyster mushroom (Pleurotus sajur-caju) and its sensory acceptability
    Lew, K.F., Goh, G.L, Son, R., Rukayadi, Y.
    International Food Research Journal, 2015;22(6):2446-2451.
    MyJurnal
    The effects of methanolic extract of Javanese turmeric (Curcuma xanthorrhiza Roxb.) at different level of concentrations on the inactivation of Bacillus cereus, Escherichia coli, Pseudomonas spp. and Staphylococcus aureus in oyster mushroom (Pleurotus sajor-caju) were investigated. This study was conducted principally for the achievement on the best combination between the
    susceptibility of C. xanthorrhiza extract on natural microflora and foodborne pathogenic bacteria with the sensory acceptability of the soaked oyster mushroom. Three different concentrations (g/ml), 0.05%, 0.50% and 5.00%, of C. xanthorrhiza extract prepared with dilution method were designed as sanitizing agent in treating the oyster mushroom at 5 minutes and 10 minutes.
    There was significance reduction in the survival of microbial load between the untreated fresh oyster mushroom and those soaked with 0.05%, 0.50% and 5.00% rhizome extract (P
    Matched MeSH terms: Pseudomonas
  20. Modulation of affinity of a marine pseudomonad for toluene and benzene by hydrocarbon exposure
    Law AT, Button DK
    Appl Environ Microbiol, 1986 Mar;51(3):469-76.
    PMID: 16347006
    Trace (microgram liter) quantities of either toluene or benzene injected into an amino-acid-limited continuous culture of Pseudomonas sp. strain T2 were utilized immediately with affinities of 2.6 and 6.8 liters g of cells h, respectively, and yielded large amounts of organic products, carbon dioxide, and cells. The immediate utilization of hydrocarbons by hydrocarbon-deprived organisms helps to establish the nutritional value of nonpolar substrates in the environment. The observation of small Michaelis constants for toluene transport led to tests of metabolic competition between hydrocarbons; however, competitive inhibition of toluene metabolism was not found for benzene, naphthalene, xylene, dodecane, or amino acids. Benzene and terpenes were inhibitory at milligram liter concentrations. Toluene was metabolized by a strongly inducible system when compared with benzene. The capacity of toluene to effect larger affinity values increased with exposure time and concentration. The kinetics of induction suggested saturation phenomena, resulting in an induction constant, K(ind), of 96 mug of toluene liter. Maximal induction of amino-acid-grown cells required about 80 h, with the affinity reaching 317 liters g of cells h.
    Matched MeSH terms: Pseudomonas
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