Displaying publications 161 - 180 of 619 in total

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  1. Wan Razarinah W, Zalina MN, Noorlidah Abdullah
    Sains Malaysiana, 2015;44:309-316.
    The study monitored the characteristics of the leachate collected from ten different landfills and presented the experimental work for the treatment of leachate by immobilized Trametes menziesii. Variation in biological oxygen demand (BOD), chemical oxygen demand (COD) and ammoniacal nitrogen (NH3-N) showed that the age of the leachate has a significant effect on its characteristics and composition. The BOD5/COD ratio tends to decrease as the age of leachate increases, varying from 0.71 for a relatively 'fresh' leachate to 0.62 for an older (more stabilized) one. Variations in the characteristics of the leachate suggested that these leachates are difficult to treat. The principal pollutants in the leachate samples were organic and ammonia loads. Treatment of leachate using immobilized Trametes menziesii achieved 89.14 and 2.11% removals for leachate BOD5 and COD, respectively. These findings suggested that using immobilized Trametes menziesii can remove promising percentage of BOD and COD leachate.
    Matched MeSH terms: Nitrogen
  2. Wan Mohd Razi Idris, Sahibin Abdul Rahim, Mohd Talib Latif, Zulfahmi Ali Rahman, Tukimat Lihan, Low YC, et al.
    Kajian yang dijalankan ini bertujuan untuk menilai indeks kualiti air di sekitar kawasan lombong di Sungai Pelepah Kanan, Kota Tinggi, Johor. Sebanyak enam stesen pensampelan telah dipilih dari bahagian hulu ke hilir sungai ini untuk menilai indeks kualiti air di sepanjang sungai tersebut. Tiga replikasi sampel telah diambil daripada setiap stesen pensampelan. Pengambilan sampel dilakukan pada dua musim yang berbeza iaitu musim kering (Julai) dan musim hujan (Disember) 2007. Parameter in-situ yang ditentukan dalam kajian ini ialah suhu, pH, oksigen terlarut (DO) dan konduktiviti. Parameter ex-situ yang dianalisis dalam makmal ialah turbiditi air, jumlah pepejal terampai (TSS), keperluan oksigen biokimia (BOD), keperluan oksigen kimia (COD) dan ammonia nitrogen (NH3-N). Berdasarkan Piawaian Interim Kualiti Air Kebangsaan Malaysia (INWQS) hasil kajian yang diperolehi menunjukkan semua stesen pensampelan di Sungai Pelepah Kanan pada bulan Julai berada dalam kelas I kecuali oksigen terlarut dan pH berada dalam kelas II. Selain itu, hasil kajian pada bulan Disember juga menunjukkan semua parameter fiziko-kimia berada dalam kelas I-II kecuali pH berada dalam kelas III. Ujian korelasi menunjukkan terdapat hubungan bererti antara parameter-parameter fiziko-kimia yang di tertentukan. Analisis Indeks Kualiti Air (IKA) menunjukkan nilai purata IKA pada bulan Julai adalah 96.88 (kelas I) manakala pada bulan Disember telah merosot ke 84.03 (kelas II). Berdasarkan kepada nilai IKA dan perbandingan dengan INWQS, indeks kualiti air Sungai Pelepah Kanan adalah berada pada tahap yang bersih dan kurang mengalami pencemaran yang serius daripada aktiviti antropogenik mahupun pencemaran secara semula jadi.
    Matched MeSH terms: Nitrogen
  3. Khairiah Haji Badri, Muhammad Syukri Ngah
    Sains Malaysiana, 2015;44:861-867.
    An investigation on a batch production of palm kernel oil polyol (PKO-p) was conducted via esterification and condensation.
    The process design was thoroughly studied as a preliminary step for future upscaling. The process variables included
    necessity of vacuum pump, controlling of heating rate, recording the production time, nitrogen gas flow and agitator
    speed. About 250 mL PKO-p was successfully synthesized within 3 h. Vacuum pressure was applied to haul out moisture
    from the system. The control of heating rate and production time are vital to avoid sudden oxidation.
    Matched MeSH terms: Nitrogen
  4. Zhang MW, Yeoh FY, Du Y, Lin KA
    Sci Total Environ, 2019 Aug 15;678:466-475.
    PMID: 31077925 DOI: 10.1016/j.scitotenv.2019.04.295
    As methyltheobromine (MTB) has been increasingly detected in wastewater, it would be necessary to develop more intensive and effective approaches to remove MTB. As Co species immobilized on carbonaceous materials appears as a promising catalyst, doping carbon with nitrogen has been also validated to significantly enhance catalytic activities for Oxone activation. Therefore, it is desired to develop a composite of immobilizing Co species on N-doped carbonaceous supports for activating Oxone to degrade MTB. Unfortunately, very few studies have demonstrated such composites for activating Oxone to degrade MTB as this type of composites are conventionally prepared via complex procedures. Alternatively, this study aims to develop such a composite conveniently by using a cobaltic coordination polymer (CP) as a precursor. Specifically Co2+ and 4,4-bipyridine (BIPY) are selected for formulating a special one-dimensional CP, which is then carbonized to convert Co to Co nanoparticles (NPs) and transform BIPY to carbon nitride (CN) matrices. Because of 1-D coordinated structure of CoBIPY, the resulting magnetic Co NPs are well-distributed and protected within CN to form a magnetic Co-embedded carbon nitride composite (MCoCN). In comparison to pristine CN and Co3O4, MCoCN exhibits much higher catalytic activities to activate Oxone for degrading MTB completely within 7 min. MCoCN also shows a much lower activation energy of 24.6 kJ/mol than other reported catalysts for activating Oxone to degrade MTB. The findings of this study validate that the 1-D coordination polymer of CoBIPY is a useful precursor to prepare MCoCN for effectively activating Oxone to degrade MTB.
    Matched MeSH terms: Nitrogen
  5. Mian S, Ishak SD, Noordin NM, Kader MA, Abduh YM, Khatoon H, et al.
    Data Brief, 2020 Apr;29:105287.
    PMID: 32123713 DOI: 10.1016/j.dib.2020.105287
    The data collection was initiated to evaluate the effects of supplementary phospholipid to non-fishmeal based diet in order to make functional diets for the Malaysian Mahseer, Tor tambroides. Four iso-nitrogenous and iso-lipidic diets were formulated to consist 100% fishmeal (FM100), 0% fishmeal or full fishmeal replacement (FM0), and 0% fishmeal supplemented with 4% phospholipids (FM0+4%PL), 6% phospholipids (FM0+6%PL). A 60-day feeding trial was conducted and data collection was carried out for the following parameters; growth indices, somatic parameters, whole body nutrient composition, muscle fatty acid composition, haematocrit value and serum lysozyme activity. Fish fed FM0 diets showed significantly poor performance (P 
    Matched MeSH terms: Nitrogen
  6. Zainudin MH, Mustapha NA, Maeda T, Ramli N, Sakai K, Hassan M
    Waste Manag, 2020 Apr 01;106:240-249.
    PMID: 32240940 DOI: 10.1016/j.wasman.2020.03.029
    Biochar has proven to be a feasible additive for mitigating nitrogen loss during the composting process. This study aims to evaluate the influence of biochar addition on bacterial community and physicochemical properties changes, including ammonium (NH4+), nitrite (NO2-) and nitrate (NO3-) contents during the composting of poultry manure. The composting was carried out by adding 20% (w/w) of biochar into the mixture of poultry manure and rice straw with a ratio of 2:1, and the same treatment without biochar was prepared as a control. The finished product of control compost recorded the high contents of NO2- and NO3- (366 mg/kg and 600 mg/kg) with reduced the total NH4+ content to 10 mg/kg. Meanwhile, biochar compost recorded a higher amount of total NH4+ content (110 mg/kg) with low NO2- and NO3- (161 mg/kg and 137 mg/kg) content in the final composting material. The principal component analysis showed that the dynamics of dominant genera related to Halomonas, Pusillimonas, and Pseudofulvimonas, all of which were known as nitrifying and denitrifying bacteria, was significantly correlated with the dynamic of NO2- and NO3- content throughout the composting process. The genera related to Pusillimonas, and Pseudofulvimonas appeared as the dominant communities as the NO2- and NO3- increased. In contrast, as the NO2- and NO3- concentration decreased, the Halomonas genus were notably enriched in biochar compost. This study revealed the bacterial community shifts corresponded with the change of physicochemical properties, which provides essential information for a better understanding of monitoring and improving the composting process.
    Matched MeSH terms: Nitrogen
  7. Ling Ty, Lee Nyanti, Theresa Muan, Jongkar Grinang, Siong-fong Sim, Aazani Mujahid
    Sains Malaysiana, 2016;45:157-166.
    Determining the water quality of Bakun Reservoir 13 months after it operates at full supply level is crucial for better understanding of changes in the physicochemical parameters, which may enable the prediction of its effects on the survival of aquatic life in the reservoir. This study determined 13 physicochemical parameters at six stations within the reservoir at fixed depths. The results showed that the minimum 5 mg/L of dissolved oxygen (DO) required for sensitive aquatic organisms was recorded at 6 m depth. However, DO was not detectable at depths exceeding 7 m. The water was acidic at depths of more than 10 m. Turbidity and total suspended solids increased corresponding with depth. Inorganic nitrogen were predominantly in the form of ammonia-nitrogen, creating an unhealthy environment for aquatic life. Concentration of Chl-a was significantly higher at the subsurface water than 30 m depth in four out of six stations. The present study shows changes in water quality as compared to the pre-impounded period and 15 months after the filling phase, in particular, stratification of dissolved oxygen, thermocline conditions and alkalinity. The changes varied according to the distance from the dam and may have been influenced by existing land developments within the area such as the construction of the Murum Hydroelectric Dam, oil palm plantations and timber concessionares. Though the water quality might have deteriorated, further study is needed to determine if this condition will prolong.
    Matched MeSH terms: Nitrogen
  8. Izyan Munirah M. Zaideen, Suhaimi Suratman, Norhayati Mohd Tahir
    Sains Malaysiana, 2017;46:1513-1520.
    This study investigates the spatial variation of water quality parameters in Sungai Setiu Basin at ten different locations from March 2010 to February 2011. The water quality was assessed using the Water Quality Index by Malaysian Department of Environment (DOE-WQI) and classified according to the Malaysia Interim National Water Quality Standard (INWQS). Six water quality parameters embedded in the DOE-WQI were dissolved oxygen (DO), biochemical oxygen demand (BOD), chemical oxygen demand (COD), pH, ammoniacal nitrogen (AN) and total suspended solid (TSS). In addition, this study also examined the changes in water quality over the past 10 years by comparing the present water quality to the previous works. The overall mean WQI value obtained was 84.0 which indicate that the Sungai Setiu basin is in clean condition and all measured water quality parameters gave value within the permissible limits of the INWQS classification except for pH which fall in Class III. It can be concluded that water quality in Sungai Setiu does not varies greatly over a decade. Hence continuous monitoring is needed to improve the water quality and minimize water pollution.
    Matched MeSH terms: Nitrogen
  9. Bharati J, Jha V
    Semin Dial, 2020 11;33(6):490-498.
    PMID: 33030777 DOI: 10.1111/sdi.12924
    Dialysis adequacy is conventionally quantified as net urea clearance. Single pool (sp) Kt/Vurea remains the best studied measure of dialysis adequacy globally. Other measures such as fluid status control, anemia correction, and mineral metabolism are monitored variably. Increasing use of hemodiafiltration across Europe and many parts of Japan and Australia is predicated on studies showing better patient survival with middle molecule clearance. Apart from local clinical practice guidelines, the income level and public health policy of a country determine quality of dialysis services. Among developed nations, small solute clearance adequacy targets are achieved with high frequency. In the United States, dialysis adequacy target is met by focussing on high blood flow rates and large dialyzer size, sometimes at the cost of session time. In Japan, Australia, and Germany, session length is given importance. Dialysis adequacy reporting is restricted and inconsistent in developing nations. The Gulf Cooperation Council countries, Russia and Malaysia, respectively, are close to achieving dialysis adequacy target (spKt/Vurea ≥1.2) universally in their dialysis populations. Patient-reported outcomes are typically measured only in developed countries. Patient survival on dialysis, partly linked to dialysis adequacy, varies greatly around the world, with Japan having the best survival rates. Until the development of better markers of dialysis adequacy, universal consistency in reporting of conventional parameters with a focus on patient-reported measures should be endeavored.
    Matched MeSH terms: Blood Urea Nitrogen
  10. Sanjaya EH, Chen Y, Guo Y, Wu J, Chen H, Din MFM, et al.
    Bioresour Technol, 2022 Feb;346:126622.
    PMID: 34958906 DOI: 10.1016/j.biortech.2021.126622
    The simultaneous partial nitritation, anammox, denitrification, and COD oxidation (SNADCO) method was successfully carried out in an air-lift moving bed biofilm reactor (AL-MBBR) with cylinders carriers for the treatment of digested fish processing wastewater (FPW). Synthetic wastewater was used as substrate at stage 1. It changed into the digested FPW with dilution variation in order to increase the nitrogen and COD loading rates. With influent concentration of NH4+-N of 909 ± 101 mg-N/L and COD of 731 ± 26 mg/L, the nitrogen removal efficiency was 86.8% (nitrogen loading rate of 1.21 g-TN/L/d) and the COD removal efficiency was 50.5% (COD loading rate at 0.98 g-COD/L/d). This study showed that the process has the advantages in treating the real high ammonia concentration of digested wastewater containing organic compounds. The nitritation and anammox route was predominant in nitrogen removal, while COD oxidation and microbe proliferation played the main role in COD removal.
    Matched MeSH terms: Nitrogen
  11. Detho A, Daud Z, Rosli MA, Awang H
    J Air Waste Manag Assoc, 2022 01;72(1):69-75.
    PMID: 33689591 DOI: 10.1080/10962247.2021.1894267
    Landfill leachate is a liquid generated due to rainwater percolation through the waste in a landfill or dumping site that may contain high levels of organic matter, both biodegradable and non-biodegradable, which are the major sources of water pollution. Chemical oxygen demand (COD) and Ammoniacal Nitrogen (NH3-N) contents have been relevant indicators of severity and pollution potential of landfill leachate. The reductions of COD and NH3-N were investigated in this study using different combinations of media ratios of green mussel (GM) and zeolite (ZEO). Generally, ZEO is considered as a renowned adsorbent but with a relatively high in cost. In Malaysia, mussel shell is abundantly available as a by-product from the seafood industry, is regarded as waste, and is mostly left at the dumpsite to naturally deteriorate. Its quality and availability make GMs a cost-effective material. In this research study, leachate samples were characterized and found to contain high concentrations of COD and NH3-N. The adsorption process was conducted to find out the best combination media ratio between GM and ZEO. The removing efficiency was determined at different amounts of composite media ratios. The optimal adsorbent mixture ratios between (GM: ZEO) of 1.0:3.0 and 1.5:2.5 were considered as a more efficient technique in removing COD and NH3-N compared to exploiting these adsorbents individually. The optimal extenuation removal reduction was found at an approximately 65% of COD and 78% of NH3-N. The adsorption Isotherm Langmuir model exhibited a better fit with high regression coefficient for COD (R2 = 0.9998) and NH3-N (R2 = 0.9875), respectively. This means that the combination of GM: ZEO adsorption of landfill leachate in this analysis is homogeneous with the monolayer. The mixture of GMs and ZEO was observed to provide an alternative medium for the reduction of COD and NH3-N with comparatively lower cost.Implications: The concentration of organic constituents (COD) and ammoniacal nitrogen in stabilized landfill leachate have significantly strong influences of human health and the environment. The combination of mixing media green mussel and zeolite adsorbent enhancing organic constituents (COD) and ammoniacal nitrogen reduction efficiency from leachate. This would be greatly applicable in future research as well as conventionally minimizing high cost materials like zeolite, thereby lowering the operating cost of leachate treatment.
    Matched MeSH terms: Nitrogen
  12. Hui KC, Ang WL, Yahya WZN, Sambudi NS
    Chemosphere, 2022 Mar;290:133377.
    PMID: 34952025 DOI: 10.1016/j.chemosphere.2021.133377
    The present work demonstrates the coupling of titanium dioxide, TiO2 nanoparticles (TNP) with N-doped, Bi-doped, and N-Bi co-doped rice husk-derived carbon dots (CDs) via a facile dispersion method, forming respective photocatalyst composites of CDs/TNP, N-CDs/TNP, Bi-CDs/TNP and N-Bi-CDs/TNP. Characterization analyzes verified the successful incorporation of respective CDs samples into TNP, forming photocatalyst composite with narrowed band gap and quenched photoluminescence intensity. Photocatalytic activity of TNP and the respective composites was investigated for photodegradation of diclofenac (DCF) under both simulated sunlight and natural sunlight irradiation. The as-prepared N-Bi-CDs/TNP composite showed the best photocatalytic performance among all composites, able to completely degrade 5 ppm of DCF within 60 min and 180 min under both types of visible light irradiation, respectively. The N-Bi-CDs/TNP composite also showed a TOC removal efficiency up to 87.63%. N-Bi-CDs, worked as photosensitizer and electron reservoir, contributed to the outstanding photocatalytic activity of N-Bi-CDs/TNP, whereby the recombination was prolonged and light absorption was shifted towards the visible light region. Furthermore, the composite of N-Bi-CDs/TNP also demonstrated good stability and reusability over repeated degradation cycles. The photodegradation of DCF resulted into several intermediates, which were identified from LC-MS analysis. The present work could provide an insight on the application of heteroatoms doped and co-doped carbon dots in semiconductor oxide as high performance photocatalysts.
    Matched MeSH terms: Nitrogen
  13. Yavari S, Malakahmad A, Sapari NB, Yavari S, Khan E
    J Environ Manage, 2017 Nov 01;202(Pt 1):225-231.
    PMID: 28735207 DOI: 10.1016/j.jenvman.2017.07.030
    Application of urea manufacturing wastewater to teak (Tectona grandis) trees, a fast growing tropical timber plants, is an environmentally-friendly and cost-effective alternative for treatment of nitrogen-rich wastewater. However, the plant growth is strongly limited by lack of phosphorus (P) and potassium (K) elements when the plants are irrigated with wastewater containing high concentration of nitrogen (N). A greenhouse experiment was conducted to optimize the efficiency of teak-based remediation systems in terms of nutrient balance. Twelve test solutions consisted of 4 levels of P (95, 190, 570, 1140 mgL-1) and 3 levels of K (95, 190, 570 mgL-1) with a constant level of N (190 mgL-1) were applied to teak seedlings every four days during the study period. Evapotranspiration rate, nutrient removal percentage, leaf surface area, dry weight and nutrient contents of experimental plants were determined and compared with those grown in control solution containing only N (N:P:K = 1:0:0). Teak seedlings grown in units with 1:0.5:1 N:P:K ratio were highly effective at nutrient removal upto 47%, 48% and 49% for N, P and K, respectively. Removal efficiency of teak plants grown in other experimental units decreased with increasing P and K concentrations in test solutions. The lowest nutrient removal and plant growth were recorded in units with 1:6:0.5 N:P:K ratio which received the highest ratio of P to K. The findings indicated that teak seedlings functioned effectively as phytoremediation plants for N-rich wastewater treatment when they were being supplied with proper concentrations of P and K.
    Matched MeSH terms: Nitrogen
  14. Phang SJ, Goh JM, Tan LL, Lee WPC, Ong WJ, Chai SP
    Environ Sci Pollut Res Int, 2021 Jan;28(4):4388-4403.
    PMID: 32940840 DOI: 10.1007/s11356-020-10814-z
    Graphitic carbon nitride (g-C3N4) has been regarded as a promising visible light-driven photocatalyst ascribable to its tailorable structures, thermal stability and chemical inertness. Enhanced photocatalytic activity is achievable by the construction of homojunction nanocomposites to reduce the undesired recombination of photogenerated charge carriers. In the present work, a novel g-C3N4/g-C3N4 metal-free homojunction photocatalyst was synthesized via hydrothermal polymerization. The g-C3N4/g-C3N4 derived from urea and thiourea demonstrated admirable photocatalytic activity towards rhodamine B (RhB) degradation upon irradiation of an 18 W LED light. The viability of the photoreaction with a low-powered excitation source highlighted the economic and environmental benefits of the process. The optimal g-C3N4/g-C3N4 homojunction photocatalyst exhibited a 2- and 1.8-fold increase in efficiency in relative to pristine g-C3N4 derived from urea and thiourea respectively. The enhanced photocatalytic performance is credited to the improved interfacial transfer and separation of electron-hole pairs across the homojunction interface. Furthermore, an excellent photochemical stability and durability is displayed by g-C3N4/g-C3N4 after three consecutive cycles. In addition, a plausible photocatalytic mechanism was proposed based on various scavenging tests. Overall, experimental results generated from this study is expected to intrigue novel research inspirations in developing metal-free homojunction photocatalysts to be feasible for large-scale wastewater treatment without compromising economically. Graphical abstract.
    Matched MeSH terms: Nitrogen Compounds
  15. Ishaq A, Said MIM, Azman SB, Abdulwahab MF, Jagun ZT
    Environ Sci Pollut Res Int, 2023 Aug;30(36):86498-86519.
    PMID: 37454007 DOI: 10.1007/s11356-023-28580-z
    Untreated landfill leachate can harm the environment and human health due to its organic debris, heavy metals, and nitrogen molecules like ammonia. Microbial fuel cells (MFCs) have emerged as a promising technology for treating landfill leachate and generating energy. However, high concentrations of total ammonia-nitrogen (TAN), which includes both ammonia and the ammonium ion, can impede MFC performance. Therefore, maintaining an adequate TAN concentration is crucial, as both excess and insufficient levels can reduce power generation. To evaluate the worldwide research on MFCs using landfill leachate as a substrate, bibliometric analysis was conducted to assess publication output, author-country co-authorship, and author keyword co-occurrence. Scopus and Web of Science retrieved 98 journal articles on this topic during 2011-2022; 18 were specifically evaluated and analysed for MFC ammonia inhibition. The results showed that research on MFC using landfill leachate as a substrate began in 2011, and the number of related papers has consistently increased every 2 years, totaling 4060 references. China, India, and the USA accounted for approximately 60% of all global publications, while the remaining 40% was contributed by 70 other countries/territories. Chongqing University emerged as one of the top contributors among this subject's ten most productive universities. Most studies found that maintaining TAN concentrations in the 400-800 mg L-1 in MFC operation produced good power density, pollution elimination, and microbial acclimatization. However, the database has few articles on MFC and landfill leachate; MFC ammonia inhibition remains the main factor impacting system performance. This bibliographic analysis provides excellent references and future research directions, highlighting the current limitations of MFC research in this area.
    Matched MeSH terms: Nitrogen
  16. Xiong J, Luo R, Jia Z, Ge S, Lam SS, Xie L, et al.
    Int J Biol Macromol, 2024 Jan;256(Pt 2):128399.
    PMID: 38007014 DOI: 10.1016/j.ijbiomac.2023.128399
    To develop a green and facile adsorbent for removing indoor polluted formaldehyde (HCHO) gas, the biomass porous nanofibrous membranes (BPNMs) derived from microcrystalline cellulose/chitosan were fabricated by electrospinning. The enhanced chemical adsorption sites with diverse oxygen (O) and nitrogen (N)-containing functional groups were introduced on the surface of BPNMs by non-thermal plasma modification under carbon dioxide (CO2) and nitrogen (N2) atmospheres. The average nanofiber diameters of nanofibrous membranes and their nanomechanical elastic modulus and hardness values decreased from 341 nm to 175-317 nm and from 2.00 GPa and 0.25 GPa to 1.70 GPa and 0.21 GPa, respectively, after plasma activation. The plasma-activated nanofibers showed superior hydrophilicity (WCA = 0°) and higher crystallinity than that of the control. The optimal HCHO adsorption capacity (134.16 mg g-1) of BPNMs was achieved under a N2 atmosphere at a plasma power of 30 W and for 3 min, which was 62.42 % higher compared with the control. Pyrrolic N, pyridinic N, CO and O-C=O were the most significant O and N-containing functional groups for the improved chemical adsorption of the BPNMs. The adsorption mechanism involved a synergistic combination of physical and chemical adsorption. This study provides a novel strategy that combines clean plasma activation with electrospinning to efficiently remove gaseous HCHO.
    Matched MeSH terms: Nitrogen
  17. van Velzen R, Holmer R, Bu F, Rutten L, van Zeijl A, Liu W, et al.
    Proc Natl Acad Sci U S A, 2018 May 15;115(20):E4700-E4709.
    PMID: 29717040 DOI: 10.1073/pnas.1721395115
    Nodules harboring nitrogen-fixing rhizobia are a well-known trait of legumes, but nodules also occur in other plant lineages, with rhizobia or the actinomycete Frankia as microsymbiont. It is generally assumed that nodulation evolved independently multiple times. However, molecular-genetic support for this hypothesis is lacking, as the genetic changes underlying nodule evolution remain elusive. We conducted genetic and comparative genomics studies by using Parasponia species (Cannabaceae), the only nonlegumes that can establish nitrogen-fixing nodules with rhizobium. Intergeneric crosses between Parasponia andersonii and its nonnodulating relative Trema tomentosa demonstrated that nodule organogenesis, but not intracellular infection, is a dominant genetic trait. Comparative transcriptomics of P. andersonii and the legume Medicago truncatula revealed utilization of at least 290 orthologous symbiosis genes in nodules. Among these are key genes that, in legumes, are essential for nodulation, including NODULE INCEPTION (NIN) and RHIZOBIUM-DIRECTED POLAR GROWTH (RPG). Comparative analysis of genomes from three Parasponia species and related nonnodulating plant species show evidence of parallel loss in nonnodulating species of putative orthologs of NIN, RPG, and NOD FACTOR PERCEPTION Parallel loss of these symbiosis genes indicates that these nonnodulating lineages lost the potential to nodulate. Taken together, our results challenge the view that nodulation evolved in parallel and raises the possibility that nodulation originated ∼100 Mya in a common ancestor of all nodulating plant species, but was subsequently lost in many descendant lineages. This will have profound implications for translational approaches aimed at engineering nitrogen-fixing nodules in crop plants.
    Matched MeSH terms: Nitrogen/metabolism; Nitrogen Fixation*
  18. Raza A, Ejaz S, Saleem MS, Hejnak V, Ahmad F, Ahmed MAA, et al.
    PLoS One, 2021;16(12):e0261468.
    PMID: 34919599 DOI: 10.1371/journal.pone.0261468
    Nitrogen (N) is a macronutrient desired by crop plants in large quantities. However, hiking fertilizer prices need alternative N sources for reducing its requirements through appropriate management practices. Plant growth promoting rhizobacteria (PGPR) are well-known for their role in lowering N requirements of crop plants. This study assessed the impact of PGPR inoculation on growth, allometry and biochemical traits of chili under different N doses. Two PGPR, i.e., Azospirillum 'Er-20' (nitrogen fixing) and Agrobacterium 'Ca-18' (phosphorous solubilizing) were used for inoculation, while control treatment had no PGPR inoculation. Six N doses, i.e., 100, 80, 75, 70, 60 and 50% of the N required by chili were included in the study. Data relating to growth traits, biochemical attributes and yield related traits were recorded. Interaction among N doses and PGPR inoculation significantly altered all growth traits, biochemical attributes and yield related traits. The highest values of the recorded traits were observed for 100% N with and without PGPR inoculation and 75% N with PGPR inoculation. The lowest values of the recorded traits were noted for 50% N without PGPR inoculation. The PGPR inoculation improved the measured traits compared to the traits recorded noted in same N dose without PGPR inoculation. Results revealed that PGPR had the potential to lower 25% N requirement for chili. Therefore, it is recommended that PGPR must be used in chili cultivation to lower N requirements.
    Matched MeSH terms: Nitrogen/analysis*; Nitrogen Fixation/physiology
  19. Jong VS, Tang FE
    Water Sci Technol, 2014;70(2):352-60.
    PMID: 25051484 DOI: 10.2166/wst.2014.237
    A two-staged engineered wetland-based system was designed and constructed to treat raw domestic septage. Hydraulic loading rates (HLRs) of 8.75 and 17.5 cm/d were studied with four and eight daily dosings at the second stage of the system to investigate the influence of the regimes on septage treatment. Removal of organic matter (OM) was found to be HLR dependent, where the results indicated that the increase of HLR from 8.75 to 17.5 cm/d impaired the overall level of treatment in the wetland units. Effluent of wetland fed at HLR 17.5 cm/d presented significantly lower oxygen reduction potential and dissolved oxygen values than wetland fed at 8.75 cm/d, indicative of the occurrence of less aerobic and reductive conditions in the bed. The reoxygenation capability of the wetland units was found to be heavily affected by the dosing frequency especially under high hydraulic load (17.5 cm/d). NH3-N degradation was found to decrease with statistical importance when the wetland was flushed two times more frequently with smaller batches of influent. The number of hydraulic load fractionings did not seem to affect the level of treatments of OM and ammonia for both the wetlands fed under the lower HLR of 8.75 cm/d. Prediction of hydraulic limits and management of the feeding strategies are important in the vertical type of engineered wetlands to guarantee the treatment performance and minimize the chances of filter clogging.
    Matched MeSH terms: Nitrogen/metabolism*; Nitrogen/chemistry*
  20. Herawan SG, Hadi MS, Ayob MR, Putra A
    ScientificWorldJournal, 2013;2013:624865.
    PMID: 23737721 DOI: 10.1155/2013/624865
    Activated carbons can be produced from different precursors, including coals of different ranks, and lignocellulosic materials, by physical or chemical activation processes. The objective of this paper is to characterize oil-palm shells, as a biomass byproduct from palm-oil mills which were converted into activated carbons by nitrogen pyrolysis followed by CO2 activation. The effects of no holding peak pyrolysis temperature on the physical characteristics of the activated carbons are studied. The BET surface area of the activated carbon is investigated using N2 adsorption at 77 K with selected temperatures of 500, 600, and 700°C. These pyrolysis conditions for preparing the activated carbons are found to yield higher BET surface area at a pyrolysis temperature of 700°C compared to selected commercial activated carbon. The activated carbons thus result in well-developed porosities and predominantly microporosities. By using this activation method, significant improvement can be obtained in the surface characteristics of the activated carbons. Thus this study shows that the preparation time can be shortened while better results of activated carbon can be produced.
    Matched MeSH terms: Nitrogen/isolation & purification*; Nitrogen/chemistry*
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