Browse publications by year: 2021

  1. Yingchoncharoen T, Wu TC, Choi DJ, Ong TK, Liew HB, Cho MC
    Korean Circ J, 2021 Aug;51(8):681-693.
    PMID: 34227265 DOI: 10.4070/kcj.2021.0029
    BACKGROUND AND OBJECTIVES: Heart failure (HF) poses substantial economic burden, primarily driven by high hospitalization and mortality rates. This study aimed to understand the economic burden of HF in 4 Asian countries under varying healthcare systems.

    METHODS: This was a non-interventional, retrospective study conducted in South Korea, Taiwan, Thailand and Malaysia through medical chart review. Eligible patients included those who had either ≥1 hospitalization or ≥2 outpatient visits from January 1st to December 31st, 2014, and at least one year of follow-up. Resource use and direct healthcare costs (adjusted to 2015 USD) of HF were assessed. HF costs for subgroups stratified by age and sex were assessed.

    RESULTS: A total of 568 patients were recruited from South Korea (n=200), Taiwan (n=200), Thailand (n=100) and Malaysia (n=68). The proportion of patients hospitalized ranged from 20.0% to 93.5% (South Korea 20.0%, Thailand 49.0%, Malaysia 70.6%, and Taiwan 93.5%). The overall annual HF cost per patient was $2,357, $4,513, $3,513 and $1,443 in South Korea, Taiwan, Thailand, and Malaysia, respectively; hospitalized HF care costs were $10,714, $4,790, $7,181 and $1,776, respectively. The length of stay was more than 12.2 days except in Malaysia. No specific trend was observed in subgroup analysis.

    CONCLUSIONS: In Asia, HF poses significant economic burden and hospitalization has emerged as the major cost driver among healthcare costs. A streamlined treatment strategy reducing hospitalization rate can minimize the economic burden.

  2. Oka Y, Doi M, Taniguchi M, Tiong SYX, Akiyama H, Yamamoto T, et al.
    Cereb Cortex, 2021 10 01;31(11):5225-5238.
    PMID: 34228058 DOI: 10.1093/cercor/bhab153
    Association projections from cortical pyramidal neurons connect disparate intrahemispheric cortical areas, which are implicated in higher cortical functions. The underlying developmental processes of these association projections, especially the initial phase before reaching the target areas, remain unknown. To visualize developing axons of individual neurons with association projections in the mouse neocortex, we devised a sparse labeling method that combined in utero electroporation and confocal imaging of flattened and optically cleared cortices. Using the promoter of an established callosal neuron marker gene that was expressed in over 80% of L2/3 neurons in the primary somatosensory cortex (S1) that project to the primary motor cortex (M1), we found that an association projection of a single neuron was the longest among the interstitial collaterals that branched out in L5 from the earlier-extended callosal projection. Collaterals to M1 elongated primarily within the cortical gray matter with little branching before reaching the target. Our results suggest that dual-projection neurons in S1 make a significant fraction of the association projections to M1, supporting the directed guidance mechanism in long-range corticocortical circuit formation over random projections followed by specific pruning.
  3. Angelakopoulos N, Galić I, Balla SB, Kiş HC, Gómez Jiménez L, Zolotenkova G, et al.
    Int J Legal Med, 2021 Nov;135(6):2423-2436.
    PMID: 34228192 DOI: 10.1007/s00414-021-02656-2
    The diagnostic accuracy of the I3M to assess the legal age of 18 years has already been tested in several specific-population samples. The left lower third molar has been extensively used for discriminating between minors and adults. This research aimed to compare the usefulness of lower third molar maturity indexes, from both left and right side (I3ML and I3MR), in samples originating from four distinct continents in order to examine possible differences in their accuracy values. For this purpose, a sample of 10,181 orthopantomograms (OPGs), from Europe, Africa, Asia and America, was analysed and previously scored in other studies. The samples included healthy subjects with no systemic disorders with both third molars and clear depicted root apices. Wilcoxon Signed Rank test for left and right asymmetry did not show any significant differences. Data about sensitivity, specificity, predictive values, likelihood ratio and accuracy were pooled together and showed similar results for I3ML and I3MR, respectively. In addition, all these quantities were high when only the I3MR was considered to discriminate between adults and minors. The present referable database was the first to pool third molar measurements using panoramic radiographs of subjects coming from different continents. The results highlighted that both I3ML and I3MR are reliable indicators for assessing the legal age of 18 years old in those jurisdictions where this legal threshold has been set as the age of majority.
    MeSH terms: Age Determination by Teeth/methods*; Ethnic Groups; Female; Humans; Male; Mandible/growth & development; Molar, Third/growth & development*; Radiography, Panoramic; Sensitivity and Specificity; Continental Population Groups*
  4. Inoue M, Isa ILM, Orita S, Suzuki-Narita M, Inage K, Shiga Y, et al.
    Spine (Phila Pa 1976), 2021 Aug 01;46(15):E810-E816.
    PMID: 34228691 DOI: 10.1097/BRS.0000000000003921
    STUDY DESIGN: An in vivo model to study the effect of an injectable hyaluronic acid (HA) hydrogel following puncture-induced lumbar disc injury in rabbits.

    OBJECTIVES: The aim of this study was to determine the efficacy of an injectable HA hydrogel to maintain disc height and tissue hydration, promote structural repair, and attenuate inflammation and innervation in the lumbar discs.

    SUMMARY OF BACKGROUND DATA: Previously, we have demonstrated that HA hydrogel alleviated inflammation, innervation, and pain to promote disc repair. Nevertheless, the effect of an injectable HA hydrogel in the lumbar disc in a weight-bearing animal model was not performed.

    METHODS: We have adopted a surgically puncture-induced disc injury at lumbar levels in a rabbit model. The discs were grouped into sham, puncture with water injection, and puncture with HA hydrogel injection. Postoperatively, we measured changes in disc height using x-ray. We used magnetic resonance imaging to assess disc degeneration on tissue hydration after euthanasia. Post-mortem, we determined histological changes, innervation (PGP9.5) and inflammation (interleukin [IL]-6, IL-1β, and tumor necrosis factor [TNF]-α) in the discs.

    RESULTS: We have demonstrated a significant reduction of disc height and T2/T1ρ mapping with histological evidence of degenerative discs, increase of innervation and inflammation in puncture-induced disc injury over time. In the HA hydrogel group, disc height was increased at weeks four and eight. A slight increase of T2 mapping, but significantly in T1ρ mapping, was observed in the HA hydrogel group at week 8. We observed homogenous NP distribution and organised AF lamellae at week eight and a slight reduced innervation score in the treatment group. HA hydrogel significantly downregulated IL-6 expression at day 1. This, however, was only slightly reduced for IL-1β and TNF-α.

    CONCLUSION: An injectable HA hydrogel had the protective effects in suppressing the loss of disc height, promoting tissue hydration for structural repair, and attenuating inflammation and innervation to prevent further disc degeneration.Level of Evidence: N/A.

    MeSH terms: Animals; Disease Models, Animal; Magnetic Resonance Imaging; Rabbits
  5. WHO Rapid Evidence Appraisal for COVID-19 Therapies (REACT) Working Group, Shankar-Hari M, Vale CL, Godolphin PJ, Fisher D, Higgins JPT, et al.
    JAMA, 2021 Aug 10;326(6):499-518.
    PMID: 34228774 DOI: 10.1001/jama.2021.11330
    IMPORTANCE: Clinical trials assessing the efficacy of IL-6 antagonists in patients hospitalized for COVID-19 have variously reported benefit, no effect, and harm.

    OBJECTIVE: To estimate the association between administration of IL-6 antagonists compared with usual care or placebo and 28-day all-cause mortality and other outcomes.

    DATA SOURCES: Trials were identified through systematic searches of electronic databases between October 2020 and January 2021. Searches were not restricted by trial status or language. Additional trials were identified through contact with experts.

    STUDY SELECTION: Eligible trials randomly assigned patients hospitalized for COVID-19 to a group in whom IL-6 antagonists were administered and to a group in whom neither IL-6 antagonists nor any other immunomodulators except corticosteroids were administered. Among 72 potentially eligible trials, 27 (37.5%) met study selection criteria.

    DATA EXTRACTION AND SYNTHESIS: In this prospective meta-analysis, risk of bias was assessed using the Cochrane Risk of Bias Assessment Tool. Inconsistency among trial results was assessed using the I2 statistic. The primary analysis was an inverse variance-weighted fixed-effects meta-analysis of odds ratios (ORs) for 28-day all-cause mortality.

    MAIN OUTCOMES AND MEASURES: The primary outcome measure was all-cause mortality at 28 days after randomization. There were 9 secondary outcomes including progression to invasive mechanical ventilation or death and risk of secondary infection by 28 days.

    RESULTS: A total of 10 930 patients (median age, 61 years [range of medians, 52-68 years]; 3560 [33%] were women) participating in 27 trials were included. By 28 days, there were 1407 deaths among 6449 patients randomized to IL-6 antagonists and 1158 deaths among 4481 patients randomized to usual care or placebo (summary OR, 0.86 [95% CI, 0.79-0.95]; P = .003 based on a fixed-effects meta-analysis). This corresponds to an absolute mortality risk of 22% for IL-6 antagonists compared with an assumed mortality risk of 25% for usual care or placebo. The corresponding summary ORs were 0.83 (95% CI, 0.74-0.92; P 

    MeSH terms: Aged; Cause of Death; Drug Therapy, Combination; Female; Glucocorticoids/therapeutic use; Hospitalization; Humans; Male; Middle Aged; Prospective Studies; Respiration, Artificial; Interleukin-6/antagonists & inhibitors*; Randomized Controlled Trials as Topic; Disease Progression; Coinfection; Antibodies, Monoclonal, Humanized/therapeutic use*
  6. Younossi ZM, Ong JP, Takahashi H, Yilmaz Y, Eguchi Y, El Kassas M, et al.
    PMID: 34229038 DOI: 10.1016/j.cgh.2021.06.048
    BACKGROUND & AIMS: Despite rapidly increasing NAFLD prevalence, providers' knowledge may be limited. We assessed NAFLD knowledge and associated factors among physicians of different specialties globally.

    APPROACH & RESULTS: NAFLD knowledge surveys containing 54 and 59 questions covering three domains (Epidemiology/Pathogenesis, Diagnostics, and Treatment) were completed electronically by hepatologists, gastroenterologists (GEs), endocrinologists (ENDOs) and primary care physicians (PCPs) from 40 countries comprising 5 Global Burden of Disease (GBD) super-regions. Over 24 months, 2202 surveys were completed (488 hepatologists, 758 GEs, 148 ENDOs, and 808 PCPs; 50% High-Income GBD super-region, 27% from North Africa and Middle East, 12% Southeast Asia, and 5% South Asian and Latin America). Hepatologists saw the greatest number of NAFLD patients annually: median (IQR) 150 (60-300) vs. 100 (35-200) for GEs, 100 (30-200) for ENDOs, and 10 (4-50) for PCPs (all p<0.0001). The primary sources of NAFLD knowledge acquisition for hepatologists were international conferences (33% vs. 8-26%) and practice guidelines for others (39-44%). Internet was the second most common source of NAFLD knowledge for PCPs (28%). NAFLD knowledge scores were higher for hepatologists than GEs: Epidemiology 62% vs. 53%, Diagnostics 80% vs. 73%, Treatment 61% vs. 58% (p<0.0001) and ENDOs scores were higher than PCPs: Epidemiology 70% vs. 60%, Diagnostics 71% vs. 64%, Treatment 79% vs. 68% (p<0.0001). Being a hepatologist or ENDO was associated with higher knowledge scores than GE or PCP, respectively (p<0.05). Higher NAFLD knowledge scores were independently associated with greater number of NAFLD patients seen (p<0.05).

    CONCLUSION: Despite the growing burden of NAFLD, significant knowledge gap remains for identification, diagnosis and management of NAFLD.

  7. Yu Ke Xin, Suwandini Ambalavanan, Ainor Farahin Binti Aziz, Pravina Deligannu
    MyJurnal
    Breakfast consumption is correlated with a range of positive outcomes, namely good academic performance, appropriate nutrient intake and body mass index (BMI). However, many university students have the habits of skipping breakfast and consume insufficient amount of nutrients. The aim of this study was to determine the relationship between the behaviour of skipping breakfast and dietary intake with BMI among students of a private university in Shah Alam, Malaysia. A cross sectional study was performed among 200 students, using a self-administered questionnaire. The questionnaire consisted of socio-demographics, dietary habits and 24-hour recall food intake. The data was analysed using SPSS and Nutritionist pro software. A total of 58% students (60% male and 56% female) had the habit of breakfast skipping. The common reason of student skipping breakfast is do not have time (n = 146; 73%). Most of the respondents taking more than 2500kcal daily were overweight. The results showed no significant association (p = 0.831) between breakfast skipping and BMI. There was no significant association (p > 0.05) between covariates (socio-demography, anthropometry, intakes of protein, carbohydrate, sodium, iron and vitamin C) with BMI of students. There was significant association (p = 0.001) between dietary intake (calorie) and BMI. In conclusion, it is evidenced that the dietary intake of these students influences their BMI status, instead of the behaviour of skipping breakfast. The importance of having breakfast should be emphasised among the university students, the students should prioritise their daily nutrient intake by taking regular breakfast.
  8. Nataraja Moorthy T,, Hairunnisa MAK., Collin Babat
    MyJurnal
    Background and aim: Impression evidence found in crime scenes are used for identification. Like finger impression, foot and toe impressions are currently considered as valuable clue used for gender identity. The aim of this study is to investigate the relationship between gender and toe print ridge density in Bidayuh population in Malaysian Borneo, so that the findings may be applied in real crime scenes.
    Materials and method: The sample collection was conducted at Sarawak state, Malaysian Borneo and recruited consented 100 males and 100 females of Bidayuh population, considering the inclusion and exclusion criteria. The toe impressions were collected by inking technique, following the standard procedure and five areas in five left toes and five right toes (great toe, second toe, third toe, fourth toe and little toe) were designated for analysis from each foot of the participant. The samples were analysed and calculated the toe impression ridge density using a standard magnifier. The findings were presented in the form of tables and graphs.
    Results: The result shows that the ridge density of females were found to be comparatively higher than males in all five toes on both sides. The ridge density on left toes is the not the mirror image of right toes in both genders Thus right densities show bilateral asymmetry. The statistical analysis shows that maximum sex differences observed in third toe on right (t value 7.77) and left (t value 9.473) side of this study population.
    Conclusion: The study concluded that ridge density in toe impression on Bidayuh population provided useful information on gender determination. Even the presence of a single toe impression is sufficient for crime scene application.
  9. Shaila Kabir, A B M Tofazzal Hossain, Liew, Constance Sat Lin, M. Tanveer Hossain Parash, Chandrika Murugaiah, A. H. M. Delwar, et al.
    MyJurnal
    Besides the common respiratory symptoms or viral pneumonia, COVID-19 is also presented with different neurological symptoms. Olfactory dysfunction (OD) or impairment of the sense of smell is one of the common neurological symptoms being reported in infection caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The severity varies from anosmia to microsmia or hyposmia. OD could have a potential early screening and diagnostic value besides other neurological and common respiratory symptoms. In our descriptive concise review, we aimed to elicit the manifestation of olfactory dysfunction as an early predictor of SARS-CoV-2 infection. We are also aiming to establish, OD as a quick and reliable assessment tool of COVID-19 risk, among the healthcare workers and contact tracers, which can justify self-quarantine of the person as well as recommendation for testing on a priority basis. We have also focused on, any effect to produce this symptom by the drug used for treatment in COVID-19, and if the old age of the patient showing any extra influence to develop OD in COVID-19.
  10. Maru A, Ahmed OH, Primus WC, Jeffary AV
    Sci Rep, 2021 06 15;11(1):12545.
    PMID: 34131184 DOI: 10.1038/s41598-021-91426-6
    Unbalanced utilization of nitrogen (N) rice not economically viable neither is this practice environmental friendly. Co-application of biochar and urea could reduce the unbalanced use of this N fertilizer in rice cultivation. Thus, a field study was carried out to: (i) determine the effects of chicken litter biochar and urea fertilization on N concentration in soil solution of a cultivated rice (MR219) using dielectric measurement at a low frequency and (ii) correlate soil dielectric conductivity with rice grain yield at maturity. Dielectric response of the soil samples at 20, 40, 55, and 75 days after transplanting were determined using an inductance-capacitance-resistance meter HIOKI 3522-50 LCR HiTESTER. Selected soil chemical properties and yield were determined using standard procedures. The dielectric conductivity and permittivity of the soil samples measured before transplanting the rice seedlings were higher than those for the soil samples after transplanting. This was due to the inherent nitrogen of the chicken litter biochar and the low nitrogen uptake at the transplanting stage. The soil N response increased with increasing measurement frequency and N concentration. The permittivity of the soil samples was inversely proportional to frequency but directly proportional to N concentration in the soil solution. The estimated contents of N in the soil using the dielectric conductivity approach at 1000 Hz decreased with increasing days of fertilization and the results were similar to those of soil NH4+ determined using chemical analysis. The conductivity measured within 1000 Hz and 100,000 Hz correlated positively with the rice grain yield suggesting that nitrogen concentration of the soil can be used to estimate grain yield of the cultivated rice plants.
    MeSH terms: Agriculture; Animals; Edible Grain/metabolism; Edible Grain/chemistry; Charcoal/pharmacology; Charcoal/chemistry; Chickens; Fertilizers*; Nitrogen/metabolism; Nitrogen/chemistry*; Oryza/metabolism*; Soil/chemistry*; Urea/pharmacology; Urea/chemistry
  11. Chong HY, Leow CY, Leow CH
    Int J Biol Macromol, 2021 Aug 31;185:485-493.
    PMID: 34174313 DOI: 10.1016/j.ijbiomac.2021.06.146
    Co-existence of Japanese Encephalitis virus (JEV) with highly homologous antigenic epitopes results in antibody-based serodiagnosis being inaccurate at detecting and distinguishing JEV from other flaviviruses. This often causes misdiagnosis and inefficient treatments of flavivirus infection. Generation of JEV NS1 protein remains a challenge as it is notably expressed in the form of inactive aggregates known as inclusion bodies using bacterial expression systems. This study evaluated two trxB and gor E. coli strains in producing soluble JEV NS1 via a cold-shock expression system. High yield of JEV NS1 inclusion bodies was produced using cold-shocked expression system. Subsequently, a simplified yet successful approach in generating soluble, active JEV NS1 protein through solubilization, purification and in vitro refolding of JEV NS1 protein from inclusion bodies was developed. A step-wise dialysis refolding approach was used to facilitate JEV NS1 refolding. The authenticity of the refolded JEV NS1 was confirmed by specific antibody binding on indirect ELISA commercial anti-NS1 antibodies which showed that the refolded JEV NS1 was highly immunoreactive. This presented approach is cost-effective, and negates the need for mammalian or insect cell expression systems in order to synthesize this JEV NS1 protein of important diagnostic and therapeutic relevance in Japanese Encephalitis disease.
    MeSH terms: Antibodies, Viral/metabolism*; Epitopes/immunology; Disulfides/chemistry; Encephalitis Virus, Japanese/immunology; Encephalitis Virus, Japanese/isolation & purification*; Escherichia coli/classification; Escherichia coli/genetics; Escherichia coli/growth & development*; Inclusion Bodies, Viral/immunology; Inclusion Bodies, Viral/metabolism; Solubility; Transformation, Bacterial; Protein Engineering; Viral Nonstructural Proteins/genetics*; Viral Nonstructural Proteins/immunology; Viral Nonstructural Proteins/metabolism; Viral Nonstructural Proteins/chemistry; Protein Refolding
  12. Hamidi N, Tan YR, Jawahir S, Tan EH
    BMC Health Serv Res, 2021 Jul 04;21(1):649.
    PMID: 34217293 DOI: 10.1186/s12913-021-06656-1
    BACKGROUND: Community pharmacies provide alternatives for medication procurement and other basic and minor health-related services in addition to mainstream hospitals and primary healthcare services. This study aimed to determine the characteristics of community pharmacy users and associated factors for community pharmacy utilisation in Malaysia.

    METHODS: Secondary data analysis was performed using data from the National Health and Morbidity Survey 2019, a nationwide cross-sectional household survey that used a two-stage stratified random sampling design. Adults aged 18 years and over were included in the analysis. Respondents who reported visiting the community pharmacy for health purposes two weeks prior to the study were considered as users. Complex sample descriptive statistics were used to describe the respondents' characteristics. Logistic regression analyses were employed to determine factors associated with community pharmacy utilisation.

    RESULTS: Of the 11,155 respondents interviewed, 10.3 % reported community pharmacy utilisation for health purposes. Females (OR = 1.41, 95 % CI = 1.14, 1.73), those with tertiary education (OR = 2.03, 95 % CI = 1.26, 3.29), urban dwellers (OR = 1.42, 95 % CI = 1.13, 1.79), and those with self-reported health problems (OR = 7.62, 95 % CI = 6.05, 9.59) were more likely to utilise the community pharmacy.

    CONCLUSIONS: Demographic and socioeconomic factors were important determinants of community pharmacy utilisation in Malaysia with sex, age, education level, locality, and self-reported health problems as the associated factors. These findings serve as evidence for policy interventions, crucial for improvements in accessibility to healthcare services.

    MeSH terms: Community Pharmacy Services*; Cross-Sectional Studies; Female; Humans; Malaysia; Morbidity; Pharmacies*; Surveys and Questionnaires
  13. Naing C, Wong ST, Aung HH
    Malar J, 2021 Jul 03;20(1):302.
    PMID: 34217314 DOI: 10.1186/s12936-021-03836-6
    BACKGROUND: Malaria is still a major public health problem in sub-Saharan Africa and South-east Asia. The clinical presentations of malaria infection vary from a mild febrile illness to life-threatening severe malaria. Toll like receptors (TLRs) are postulated to be involved in the innate immune responses to malaria. Individual studies showed inconclusive findings. This study aimed to assess the role of TLR4 (D299G, T399I) and TLR9 (T1237C, T1486C) in severity or susceptibility of malaria by meta-analysis of data from eligible studies.

    METHODS: Relevant case-control studies that assessed the association between TLR 4/9 and malaria either in susceptibility or progression were searched in health-related electronic databases. Quality of included studies was evaluated with Newcastle-Ottawa scale. Pooled analyses for specific genetic polymorphisms were done under five genetic models. Stratified analysis was done by age and geographical region (Asian countries vs non-Asian countries).

    RESULTS: Eleven studies (2716 cases and 2376 controls) from nine endemic countries were identified. Five studies (45.4%) obtained high score in quality assessment. Overall, a significant association between TLR9 (T1486C) and severity of malaria is observed in allele model (OR: 1.26, 95% CI: 1.08-1.48, I2 = 0%) or homozygous model (OR: 1.55, 95% CI: 1.08-2.28, I2 = 0%). For TLR9 (T1237C), a significant association with severity of malaria is observed in in heterozygous model (OR:1.89, 95% CI: 1.11-3.22, I2 = 75%). On stratifications, TLR9 (T1486C) is only significantly associated with a subgroup of children of non-Asian countries under allele model (OR: 1.25, 95% CI: 1.02-1.38), while 1237 is with a subgroup of adults from Asian countries under heterozygous model (OR: 2.0, 95% CI: 1.09-3.64, I2 = 39%). Regarding the susceptibility to malaria, TLR9 (T1237C) is significantly associated only with the children group under recessive model (OR: 2.21, 95% CI: 1.06-4.57, I2=85%) and homozygous model (OR: 1.49, 95% CI: 1.09-2.0, I2 = 0%). For TLR4 (D299G, T399I), none is significantly associated with either severity of malaria or susceptibility to malaria under any genetic models.

    CONCLUSIONS: The findings suggest that TLR 9 (T1486C and T1237C) seems to influence the progression of malaria, under certain genetic models and in specific age group of people from specific geographical region. TLR 9 (T1237C) also plays a role in susceptibility to malaria under certain genetic models and only with children of non-Asian countries. To substantiate these, future well designed studies with larger samples across endemic countries are needed.

  14. Priyanka S, Sakthivel R, Mohanapriya S, Kong F, Saat S
    ISA Trans, 2021 Jun 22.
    PMID: 34217497 DOI: 10.1016/j.isatra.2021.06.022
    This paper investigates the issue of fault-tolerant and anti-disturbance attenuation for a two-dimensional modified repetitive control system (2D MRCS) which is described by switched fuzzy systems with multiple disturbances. In particular, the multiple disturbances contain an exogenous disturbance and standard Wiener noise. Specifically, a generalized extended state observer (GESO) is incorporated with the 2D MRCS to estimate both fault and exogenous multiple disturbances so that the disturbances and faults can be attenuated in the control input. Further, the improved 2D MRCS relaxes the stability condition and provides an enhanced tracking performance. Based on the Lyapunov function approach, pole placement technique and average dwell time approach, the stability criteria for the considered system is developed in terms of linear matrix inequality (LMI). Then an algorithm for designing a GESO-based 2D MRC design is developed based on the obtained LMIs. Further, the results developed are validated in the simulation section through three numerical examples.
  15. Ait Abderrahim L, Taibi K, Boussaid M, Al-Shara B, Ait Abderrahim N, Ait Abderrahim S
    Toxicon, 2021 Sep;200:30-37.
    PMID: 34217748 DOI: 10.1016/j.toxicon.2021.06.018
    Microcystins (MCs) are hepatotoxic cyanotoxins implicated in several incidents of human and animal toxicity. Microcystin-(Lysine, Arginine) or MC-LR is the most toxic and encountered variant of MCs where oxidative stress plays a key role in its toxicity. This study investigated the oxidative damages induced in the liver and heart of Balb/C mice by an intraperitoneal injected acute dose of MC-LR. Thereafter, the potential protective effect of garlic (Allium sativum) extract supplementation against such damages was assessed through the evaluation of oxidative stress and cytotoxicity markers. Lipid peroxidation (LPO), carbonyl content (CC), glutathione content (GSH), alkaline phosphatase activity (ALP), lactate dehydrogenase (LDH) and sorbitol dehydrogenase (SDH) activities were measured. Results showed important oxidative damages in hepatic and cardiac cells of mice injected with the toxin. However, these damages have been significantly reduced in mice supplemented with garlic extract. Thus, this study demonstrated for the first time the effective use of garlic as an antioxidant agent against oxidative damages induced by MC-LR. As well, this study supports the use of garlic as a potential remedy against pathologies related to toxic agents.
    MeSH terms: Animals; Antioxidants/metabolism; Garlic*; Liver/metabolism; Marine Toxins; Oxidative Stress; Mice
  16. Tan FHP, Ting ACJ, Leow BG, Najimudin N, Watanabe N, Azzam G
    J Ethnopharmacol, 2021 Oct 28;279:114389.
    PMID: 34217797 DOI: 10.1016/j.jep.2021.114389
    ETHNOPHARMACOLOGICAL RELEVANCE: Danshen water extract (DWE), obtained from the Salvia miltiorrhiza Bunge (Family Lamiaceae) root, is usually employed in Chinese traditional medicine as treatment to cardiovascular ailments and cerebrovascular diseases. Intriguingly, the extract was also found to contain vast beneficial properties in Alzheimer's disease (AD) treatment.

    AIM OF THE STUDY: Alzheimer's disease is the most significant type of neurodegenerative disorder plaguing societies globally. Its pathogenesis encompasses the hallmark aggregation of amyloid-beta (Aβ). Of all the Aβ oligomers formed in the brain, Aβ42 is the most toxic and aggressive. Despite this, the mechanism behind this disease remains elusive. In this study, DWE, and its major components, Salvianolic acid A (SalA) and Salvianolic acid B (SalB) were tested for their abilities to attenuate Aβ42's toxic effects.

    METHODS: The composition of DWE was determined via Ultra-Performance Liquid Chromatography (UPLC). DWE, SalA and SalB were first verified for their capability to diminish Aβ42 fibrillation using an in vitro activity assay. Since Aβ42 aggregation results in neuronal degeneration, the potential Aβ42 inhibitors were next evaluated on Aβ42-exposed PC12 neuronal cells. The Drosophila melanogaster AD model was then employed to determine the effects of DWE, SalA and SalB.

    RESULTS: DWE, SalA and SalB were shown to be able to reduce fibrillation of Aβ42. When tested on PC12 neuronal cells, DWE, SalA and SalB ameliorated cells from cell death associated with Aβ42 exposure. Next, DWE and its components were tested on the Drosophila melanogaster AD model and their rescue effects were further characterized. The UPLC analysis showed that SalA and SalB were present in the brains and bodies of Drosophila after DWE feeding. When human Aβ42 was expressed, the AD Drosophila exhibited degenerated eye structures known as the rough eye phenotype (REP), reduced lifespan and deteriorated locomotor ability. Administration of DWE, SalA and SalB partially reverted the REP, increased the age of AD Drosophila and improved most of the mobility of AD Drosophila.

    CONCLUSION: Collectively, DWE and its components may have therapeutic potential for AD patients and possibly other forms of brain diseases.

    MeSH terms: Alzheimer Disease/drug therapy*; Animals; Benzofurans/pharmacology*; Caffeic Acids/pharmacology*; Cell Survival/drug effects; Drosophila melanogaster; Female; Lactates/pharmacology*; Phytotherapy*; Neurons/drug effects*; Peptide Fragments/metabolism; Plant Extracts/therapeutic use; Plant Extracts/chemistry; Amyloid beta-Peptides/metabolism; PC12 Cells; Salvia miltiorrhiza/chemistry*; Rats
  17. Higgins S, Stoner L, Black K, Wong JE, Quigg R, Meredith-Jones K, et al.
    Sleep Med, 2021 08;84:294-302.
    PMID: 34217919 DOI: 10.1016/j.sleep.2021.06.014
    INTRODUCTION: Social jetlag has been reported to predict obesity-related indices, independent of sleep duration, with associations in female adolescents but not males. However, such sex-specific relationships have not been investigated in pre-adolescents.

    OBJECTIVES: To examine: (i) the relationships between sleep characteristics, including social jetlag, and obesity-related outcomes during childhood, and (ii) whether these relationships are moderated by sex.

    METHODS: This cross-sectional study included 381 children aged 9-11 years (49.6% female). Average sleep duration, social jetlag, and physical activity were assessed via wrist-worn accelerometry. Sleep disturbances were quantified from the Children's Sleep Habits Questionnaire. Obesity-related outcomes included age-specific body mass index Z-scores (zBMI) and waist-to-height ratio. Additionally % fat, total fat mass, and fat mass index were assessed via bioelectrical impedance analysis. Linear mixed models that nested children within schools were used to identify relationships among sleep characteristics and obesity-related outcomes.

    RESULTS: Positive associations between social jetlag with zBMI, % fat, and fat mass index were seen in univariable and unadjusted multivariable analyses. Following adjustments for known confounders, social jetlag remained significantly associated with zBMI (β = 0.12, p = 0.013). Simple slopes suggested a positive association in girls (β = 0.19, p = 0.006) but not in boys (β = 0.03, p = 0.703).

    CONCLUSIONS: Obesity prevention efforts, particularly in girls, may benefit from targeted approaches to improving the consistency of sleep timing in youth.

    MeSH terms: Child; Circadian Rhythm*; Cross-Sectional Studies; Female; Humans; Male; Obesity/epidemiology; Sleep; Social Behavior*; Time Factors; Body Mass Index
  18. Geng HC, Zhu HT, Yang WN, Wang D, Yang CR, Zhang YJ
    Bioorg Chem, 2021 09;114:105125.
    PMID: 34217976 DOI: 10.1016/j.bioorg.2021.105125
    The young leaves of Phyllanthus acidus (Euphorbiaceae) are commonly used as edible vegetables in Indonesia, Thailand, and India, and their water infusions as dieting aids for people trying to remain slim. However, it is regarded as a poisonous plant in Malaya, and current researches are insufficient to provide a conclusion on its toxicity and safety under large doses. In this study, we firstly found that the refined nonpolar extracts of P. acidus leaves showed significant cytotoxic effect against BEAS-2B and L02 normal cell lines with IC50 values of 2.15 and 1.64 mg/mL, respectively. Further bioactivity-guided isolation produced four new rare dichapetalins (pacidusins A-D) from the most active fraction. Their structures including absolute configurations were elucidated by extensive spectroscopic data and X-ray diffraction analysis. All the isolated dichapetalins exhibited moderate cytotoxicity against, BEAS-2B and L02 normal cell lines with IC50 values ranging from 12.44 to 22.55 μM, as well as five human cancer cell lines with IC50 values ranging from 3.38 to 22.38 μM. Furthermore, the content of the main dichapetalins in the leaves were determined by analytical HPLC, which showed that the leaves contained a very high amount of the four isolated dichapetalins with a total yield of 0.488 mg/g of dry plant material. These toxic dichapetalins may lead to adverse health effects in higher doses. Our findings indicate that the dichapetalin containing leaves may not be suitable for consumption in large quantities as food, but demonstrate their potency as anti-cancer agents for new drug discovery.
  19. Ang SC, Marret MJ, Jayanath S, Khoo WV, Takwir MFM
    Child Abuse Negl, 2021 Oct;120:105187.
    PMID: 34218125 DOI: 10.1016/j.chiabu.2021.105187
    BACKGROUND: Abusive head trauma (AHT) is a severe manifestation of physical abuse in young children. This study examines the outcome of AHT in children under 2 years and features associated with mortality and disability.

    METHODS: Retrospective chart review of 72 children under 2 years diagnosed to have AHT between 2011 and 2018 at a tertiary teaching hospital in Malaysia. Demographic variables, clinical features, results of neuroimaging and their associations with outcome were explored.

    RESULTS: The median age at presentation was 4 months, 78% were six months or younger and 68% were male. Two-thirds became unwell in the care of an alternative caregiver. Subdural hemorrhage was present in 98%. Mortality was 10%. Forty-nine survivors returned with median interval of 16 months (IQR 5-44 months) between discharge and last follow-up. At least 35% of 65 survivors were disabled with 29% having multiple disabilities. Multiple disabilities (61.1% versus 25.8%) and cognitive impairment (61.1% versus 22.6%) were significantly higher in children 3 years or older at last follow-up (p 

  20. Chong XY, Vericat D, Batalla RJ, Teo FY, Lee KSP, Gibbins CN
    Sci Total Environ, 2021 Nov 10;794:148686.
    PMID: 34218154 DOI: 10.1016/j.scitotenv.2021.148686
    A major programme of dam building is underway in many of the world's tropical countries. This raises the question of whether existing research is sufficient to fully understand the impacts of dams on tropical river systems. This paper provides a systematic review of what is known about the impacts of dams on river flows, sediment dynamics and geomorphic processes in tropical rivers. The review was conducted using the SCOPUS® and Web of Science® databases, with papers analysed to look for temporal and geographic patterns in published work, assess the approaches used to help understand dam impacts, and assess the nature and magnitude of impacts on the flow regimes and geomorphology ('hydromorphology') of tropical rivers. As part of the review, a meta-analysis was used to compare key impacts across different climate regions. Although research on tropical rivers remains scarce, existing work is sufficient to allow us to draw some very broad, general conclusions about the nature of hydromorphic change: tropical dams have resulted in reductions in flow variability, lower flood peaks, reductions in sediment supply and loads, and complex geomorphic adjustments that include both channel incision and aggradation at different times and downstream distances. At this general level, impacts are consistent with those observed in other climate regions. However, studies are too few and variable in their focus to determine whether some of the more specific aspects of change observed in tropical rivers (e.g. time to reach a new, adjusted state, and downstream recovery distance) differ consistently from those in other regions. The review helps stress the need for research that incorporates before-after comparisons of flow and geomorphic conditions, and for the wider application of tools available now for assessing hydromorphic change. Very few studies have considered hydromorphic processes when designing flow operational policies for tropical dams.
    MeSH terms: Ecosystem*; Rivers*; Floods
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