Browse publications by year: 2024

  1. Muhd Yazid NF, Che Ros MIA, Setia SA
    Cureus, 2024 Jun;16(6):e62426.
    PMID: 39011202 DOI: 10.7759/cureus.62426
    Primary cerebral lymphoma in immunocompromised patients is rare and challenging to diagnose. Its presentation can have similarities with other opportunistic diseases like cerebral toxoplasmosis and tuberculoma, to name a few, which may affect the subsequent management. Here, we report a case of a gentleman with human immunodeficiency virus (HIV) who presented with clinical features of fever, confusion, and generalized lethargy. His imaging features mimicked those of central nervous system (CNS) tuberculosis (TB), and he was treated for one. Unfortunately, the patient failed to respond to the anti-tuberculosis treatment and continued to deteriorate eventually succumbing to his illness. Brain histopathology biopsy confirmed the diagnosis of diffuse large B-cell lymphoma. We aim to illustrate the importance of a high index of suspicion with timely action taken whenever the anticipated finding or response to treatment is not observed. Multiple imaging modalities coupled with biochemistry and histopathological investigations should be considered in discriminating competing diagnoses.
  2. Zahidin MSI, Singh J, Mohammad Azmi S, Azhar A, Md Hashim MN, Zakaria AD
    Cureus, 2024 Jun;16(6):e62409.
    PMID: 39011216 DOI: 10.7759/cureus.62409
    Gastrointestinal stromal tumor (GIST) represents a rare neoplasm affecting the gastrointestinal (GI) tract and is classified as a common nonepithelial tumor within the GI tract. It originates from the interstitial cells of Cajal, and GIST typically manifests with symptoms such as abdominal pain, weight loss, and gastrointestinal bleeding. This case involves a 33-year-old male who presented with GI bleeding symptoms after eight months of treatment for anemia. Oesophagogastroduodenoscopy (OGDS) revealed a singular ulcerated mass measuring 4x4cm while a computed tomography (CT) scan identified a large fundal exophytic component extending from the gastroesophageal junction to the stomach. Subsequently, the patient underwent a laparotomy and proximal gastrectomy with Roux-en-Y reconstruction, which revealed a 12x10 cm tumor located at the fundus of the stomach. This report aims to underscore the potential for misdiagnosis in the initial presentation of GIST, emphasizing the importance of raising clinical awareness in such cases.
  3. Farhana AH, Afendi NR, Che Hashim NAH, Ismail AA, Zon EM, Rahimah AR
    PMID: 39011293 DOI: 10.51866/oa.533
    INTRODUCTION: This prospective cross-sectional study, conducted from 1 April 2022 to 31 October 2022, aimed to assess the knowledge, acceptance and concerns regarding COVID-19 vaccination among pregnant women visiting the Obstetrics and Gynaecology Department of Hospital Universiti Sains Malaysia.

    METHODS: The study included all pregnant women aged >18 years. Sociodemographic data, information related to COVID-19 and vaccination and information on the knowledge, acceptance and concerns regarding COVID-19 vaccination were collected using a validated questionnaire.

    RESULTS: Out of 420 eligible pregnant women, 412 participated in the study, yielding a response rate of 98.1%. Of the respondents, 97.1% had received a COVID-19 vaccine, while 2.9% had not. Approximately 85.2% demonstrated a good understanding of COVID-19 vaccination. Among those vaccinated, 76.8% based their decision on recommendations from healthcare providers or the Ministry of Health. Among those unvaccinated, 91.7% believed that COVID-19 vaccines could harm their pregnancy and baby. Although 51% of the respondents expressed concerns about vaccine safety, 202 still chose to be vaccinated, indicating a willingness to prioritise their health despite apprehensions.

    CONCLUSION: The study found no significant link between acceptance and good knowledge of COVID-19 vaccination. However, income and prior COVID-19 booster vaccination were strongly associated with acceptance. Despite safety concerns, 97.1% of the respondents had received a COVID-19 vaccine. This emphasises the importance of providing comprehensive information and addressing concerns to support informed decision-making among pregnant women. Healthcare providers play a vital role in guiding them through this crucial decision-making process.

  4. Rachmawati E, Riskiyah, Novindra QA, Syarifah NA, Aisy NR
    PMID: 39011294 DOI: 10.6084/m9.figshare.24878427.v1
    INTRODUCTION: A healthy lifestyle influences hypertension control. However, studies investigating the effects of lifestyle on hypertension remain elusive. This study aimed to analyse the association between lifestyle factors and hypertension control among patients with hypertension.

    METHODS: This cross-sectional exploratory study was conducted from June to December 2022 among 265 patients with hypertension from the Pusat Kesehatan Masyarakat. The status of hypertension control was assessed by checking the serial blood pressure. The physical activity (PA) level, sleep quality, stress level and eating pattern were measured using the Global Physical Activity Questionnaire; Pittsburgh Sleep Quality Index; Depression Anxiety Stress Scale-21; and 24-Hour Food Recall Questionnaire, Adolescent Food Habits Checklist and Emotional Eating Scale, respectively. Stepwise binary logistic regression and a generalised linear model were used for the statistical analysis.

    RESULTS: Approximately 72.2% of the participants had uncontrolled hypertension. The majority showed a low PA level (46%), normal stress level (94.7%), good sleep quality (80%), low caloric intake (95.5%), neutral food habit (55.5%) and low emotional eating (93.2%). Sex (P=0.030), age (P=0.018), PA level (P=0.011), sleep quality (P=0.032) and stress level (P=0.030) significantly influenced hypertension control. Specifically, moderate (odds ratio [OR]=5.868, 95% confidence interval [CI]=3.024-11.798, P=0.000) and vigorous PA levels (OR=2.188, 95% CI=1.026-4.678, P=0.042) were significantly associated with hypertension control.

    CONCLUSION: Moderate and vigorous PA levels are lifestyle factors that may play a role in controlling hypertension.

  5. Azhan KNWK, Jalil AAA, Putera SSPAS
    PMID: 39011295 DOI: 10.51866/oa.582
    INTRODUCTION: Overweight and obesity pose significant health risks, affecting social and economic well-being and potentially hindering mental health and learning outcomes. This study investigated the knowledge, attitudes, and prevalence of overweight and obesity among undergraduate students at Universiti Kuala Lumpur Royal College of Medicine (UniKL RCMP), Malaysia.

    METHODS: A total of 351 UniKL RCMP undergraduate students participated in this study. Descriptive analysis was used to evaluate the sociodemographic and educational profiles of respondents. The chi-square test was conducted to identify the relationship between the sociodemographic and educational profiles and the level of knowledge and attitudes towards overweight and obesity.

    RESULTS: The prevalence of overweight and obesity was 25.4%. The majority of the respondents possessed a good level of knowledge regarding overweight and obesity (n=316, 90%). The respondents' programme was significantly associated with their level of knowledge (P=0.001).

    CONCLUSION: This study provides important information to university authorities, students, lecturers, parents and other stakeholders in education and health sectors about the impacts of overweight and obesity on the physical, social and academic welfare of university students.

  6. Raja-Ismail RI, Badlishah-Sham SF, Nik-Nasir NM, Isa MR
    PMID: 39011296 DOI: 10.51866/oa.480
    INTRODUCTION: Self-care practices among patients with hypertension have been shown to improve blood pressure control. Video-based interventions (VBIs) are helpful in enhancing patients' selfcare practices. However, validated VBIs in the Malay language for patients in primary care settings are scarce. This study aimed to develop and validate a VBI series in the Malay language to educate patients with hypertension on self-care practices in primary care settings.

    METHODS: This study was conducted in three phases: (1) pre-production, (2) production and (3) post-production. The pre-production phase involved designing the storyboard and scripts, which underwent content validation by content experts and subsequently by patients with hypertension. Once the storyboards and scripts achieved acceptable consensus, the videos were recorded (production phase). The post-production phase included video editing and face validation among patients with hypertension. Statistical analysis included the calculation of the item-level content validation index (I-CVI) and item-level face validation index (I-FVI) during content and face validation, respectively.

    RESULTS: The storyboards and scripts for five videos were developed. The I-CVI of all videos was 1.0 after two rounds of content validation among six content experts. The I-CVI of all videos was 1.0 among five patients with hypertension. Five videos were recorded and edited, achieving an I-FVI of 1.0 during face validation among 10 patients.

    CONCLUSION: A VBI series consisting of five videos was developed and validated for use among patients with hypertension in primary care settings to improve their knowledge of self-care practices.

  7. Xuan W, Phongsatha T, Hao L, Tian K
    Front Public Health, 2024;12:1382687.
    PMID: 39011330 DOI: 10.3389/fpubh.2024.1382687
    OBJECTIVE: To enhance individuals' sustained intention to use health science popularization videos, this study investigated the path relationships and influencing mechanisms of health science popularization video factors on users' perceived value, expectancy confirmation, enjoyment, satisfaction, trust, and continuous usage intention based on the cognitive-affective-conative and expectation-confirmation model theoretical framework.

    METHODS: This study adopted a cross-sectional design and collected data using self-administered questionnaires. The hypotheses were analyzed using the smart partial least squares (Smart-PLS) structural equation modeling method with a dataset containing 503 valid responses. Subsequently, comprehensive data analysis was conducted.

    RESULTS: Blogger and video quality factors present in health science popularization videos substantially influenced users' perceived value (p 

    MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Male; Middle Aged; Models, Theoretical; Surveys and Questionnaires; Video Recording; Intention*; Trust; Young Adult
  8. Mat Zainal MK, Liew AKC, Abdullah D, Soo E, Abdul Hamid B, Ramlee RAM
    Dent Traumatol, 2024 Aug;40(4):460-469.
    PMID: 38459669 DOI: 10.1111/edt.12939
    BACKGROUND/AIM: Mouthguards are crucial for protecting athletes against orofacial injuries, yet concerns persist regarding their potential impact on oral functions. This study aimed to investigate the effects of sports mouthguards on oral functions and speech over time.

    MATERIAL AND METHODS: Thirty national rugby players received custom-fitted mouthguards. Questionnaire responses and speech recordings were collected before mouthguard use and at various intervals after using mouthguards: immediately, 1 week, 2 month, and 6 months. Spectrographic analysis was performed to measure voice onset time (VOT) for /p, b, t, d/ phonemes. Questionnaire responses were assessed with Friedman's test, while VOT changes were examined using one-way repeated measure analysis of variance.

    RESULTS: Compliance with mouthguard use improved during training and competitions, with consistent wear reported during matches. Over time, speaking difficulties and lisping decreased significantly (p 

    MeSH terms: Adult; Equipment Design*; Humans; Male; Mouth Protectors*; Surveys and Questionnaires; Speech*; Sports Equipment
  9. Irshad MA, Abdullah, Latif M, Nasim I, Nawaz R, Zahoor AF, et al.
    Ecotoxicol Environ Saf, 2024 Aug;281:116616.
    PMID: 38917589 DOI: 10.1016/j.ecoenv.2024.116616
    The urgent need to address the severe environmental risk posed by chromium-contaminated industrial wastewater necessitates the development of eco-friendly cleanup methodologies. Utilizing the Ficus benghalensis plant extracts, the present study aims to develop green zinc oxide nanoparticles for the removal of Cr metal ions from wastewater. The leaves of Ficus benghalensis, often known as the banyan tree, were used to extract a solution for synthesizing ZnO NPs. These nanoparticles were developed with the goal of efficiently eliminating chromium (Cr) from industrial effluents. Batch studies were carried out to assess the efficiency of these synthesized ZnO NPs in treating leather industrial effluent, with aiming for optimal chromium removal. This involved measuring the nanoparticles' capacity to adsorb Cr ions from wastewater samples by comparing chromium levels before and after treatment. Removal efficiency for Cr was estimated through the batches such as optimization of pH, contact time, initial Cr concentration and sorbent dose of ZnO NPs were of the batches. These synthesized ZnO NPs were found to be successful in lowering chromium levels in wastewater to meet permissible limit. The nanoparticles exhibited their highest absorption capacity, reaching 94 % (46 mg/g) at pH 4, with a contact time of 7 hours with the optimum sorbent dose of 0.6 g/L. Hence, the excellent adsorption capabilities of these nanoparticles, together with their environmentally benign manufacturing technique, provide a long-term and efficient solution for chromium-contaminated wastewater treatment. Its novel nature has the potential to significantly improve the safety and cleanliness of water ecosystems, protecting the both i.e. human health and the environment.
    MeSH terms: Adsorption; Industrial Waste/analysis; Waste Disposal, Fluid/methods; Water Purification/methods; Plant Leaves/chemistry; Nanoparticles/chemistry; Metal Nanoparticles/chemistry
  10. Wong WC, Goh YK, Wong CK, Goh YK, Marzuki NF, Choo CHY, et al.
    Plant Dis, 2024 Jul;108(7):1982-1986.
    PMID: 38937876 DOI: 10.1094/PDIS-07-23-1426-SC
    Ganoderma boninense is a basidiomycete pathogen of African oil palm (Elaeis guineensis) and the causal agent of basal stem rot (BSR) disease, which is the most destructive fungal disease of oil palm in Southeast Asia. The disease is fatal for infected palms and can result in 50 to 80% losses in oil yields because of a reduction in productive life span and a yield decline of infected oil palms. In this study, G. boninense isolates collected from different locations and planting blocks with different palm ages were molecularly characterized using microsatellite genotyping. Results showed high pathogen genetic diversity (He = 0.67 to 0.74) among planting blocks and between oil palm estates. Two nearby planting blocks with similar planting ages (i.e., 1999 and 2001) had a similar percentage of BSR incidence (>20%) but showed distinct Ganoderma genetic structure as detected using STRUCTURE. Similar results were obtained from another trial site where planting blocks differing in planting age but located only less than 1 km apart showed a diverse genetic background. The pathogen genetic admixture of the oldest planting (>30% BSR incidence) differed significantly from the younger planting (1.8 to 2.8% BSR incidence, breeding trial block), suggesting that the host-pathogen genotype interaction may impact the Ganoderma genetic variation over time. The genetic structure of G. boninense, as revealed in this study, implies positive selection resulting from the pathogen genetic variation, host-pathogen interaction, and possible introductions of novel genetic variants (through spores) from adjacent plantings. These findings offer new insights into the genetic changes of G. boninense over time. The information is essential to design disease management strategies and breeding for BSR resistance in oil palm.
    MeSH terms: Genotype; Malaysia; Genetic Variation*; Microsatellite Repeats/genetics
  11. Khatoon H, Mohd Faudzi SM
    Eur J Med Chem, 2024 Jul 13;276:116675.
    PMID: 39004020 DOI: 10.1016/j.ejmech.2024.116675
    Antimicrobial resistance (AMR) has emerged as a long-standing global issue ever since the introduction of penicillin, the first antibiotic. Scientists are constantly working to develop innovative antibiotics that are more effective and superior. Unfortunately, the misuse of antibiotics has resulted in their declining effectiveness over the years. By 2050, it is projected that approximately 10 million lives could be lost annually due to antibiotic resistance. Gaining insight into the mechanisms behind the development and transmission of AMR in well-known bacteria including Escherichia coli, Bacillus pumilus, Enterobacter aerogenes, Salmonella typhimurium, and the gut microbiota is crucial for researchers. Environmental contamination in third world and developing countries also plays a significant role in the increase of AMR. Despite the availability of numerous recognized antibiotics to combat bacterial infections, their effectiveness is diminishing due to the growing problem of AMR. The overuse of antibiotics has led to an increase in resistance rates and negative impacts on global health. This highlights the importance of implementing strong antimicrobial stewardship and improving global monitoring, as emphasized by the World Health Organization (WHO) and other organizations. In the face of these obstacles, quinoxaline derivatives have emerged as promising candidates. They are characterized by their remarkable efficacy against a broad spectrum of harmful bacteria, including strains that are resistant to multiple drugs. These compounds are known for their strong structural stability and adaptability, making them a promising and creative solution to the AMR crisis. This review aims to assess the effectiveness of quinoxaline derivatives in treating drug-resistant infections, with the goal of making a meaningful contribution to the global fight against AMR.
  12. Rasel MA, Abdul Kareem S, Kwan Z, Faheem NAA, Han WH, Choong RKJ, et al.
    Comput Biol Med, 2024 Jul 13;179:108851.
    PMID: 39004048 DOI: 10.1016/j.compbiomed.2024.108851
    In dermoscopic images, which allow visualization of surface skin structures not visible to the naked eye, lesion shape offers vital insights into skin diseases. In clinically practiced methods, asymmetric lesion shape is one of the criteria for diagnosing Melanoma. Initially, we labeled data for a non-annotated dataset with symmetrical information based on clinical assessments. Subsequently, we propose a supporting technique-a supervised learning image processing algorithm-to analyze the geometrical pattern of lesion shape, aiding non-experts in understanding the criteria of an asymmetric lesion. We then utilize a pre-trained convolutional neural network (CNN) to extract shape, color, and texture features from dermoscopic images for training a multiclass support vector machine (SVM) classifier, outperforming state-of-the-art methods from the literature. In the geometry-based experiment, we achieved a 99.00 % detection rate for dermatological asymmetric lesions. In the CNN-based experiment, the best performance is found 94 % Kappa Score, 95 % Macro F1-score, and 97 % weighted F1-score for classifying lesion shapes (Asymmetric, Half-Symmetric, and Symmetric).
  13. Whba R, Su'ait MS, Whba F, Sahinbay S, Altin S, Ahmad A
    Int J Biol Macromol, 2024 Sep;276(Pt 1):133796.
    PMID: 39004255 DOI: 10.1016/j.ijbiomac.2024.133796
    Natural rubber (NR) and its derivatives play indispensable roles in various industries due to their unique properties and versatile applications. However, the widespread utilization of NR faces intrinsic challenges such as limited mechanical strength, poor resistance to heat and organic solvent, poor electrical conductivity, and low compatibility with other materials, prompting researchers to explore enhancing its performance. Modified NRs (MNRs) like cyclization, deproteinization, chlorination, epoxidation, or grafting NR demonstrated a few enhanced merits compared to NR. However, various strategies, such as blending, vulcanization, crosslinking, grafting, plasticization, reinforcement, and nanostructuring, overcame most drawbacks. This review comprehensively examines these challenges and delves into the modification strategies employed to enhance the properties and expand the applications of NR and its derivatives. Furthermore, the review explores future visions for the NR industry, emphasizing integrating advanced modification techniques, adopting sustainable practices, and promoting circular economy principles. By elucidating the inherent challenges, outlining effective modification strategies, and envisioning future trajectories, this review provides valuable insights for stakeholders seeking to navigate and contribute to the sustainable development of the NR sector.
  14. Veronica N, Lee ESM, Heng PWS, Liew CV
    Int J Pharm, 2024 Aug 15;661:124467.
    PMID: 39004293 DOI: 10.1016/j.ijpharm.2024.124467
    Tablet disintegration is crucial for drug release and subsequent systemic absorption. Although factors affecting the disintegrant's functionality have been extensively studied, the impact of wet granulation on the performance of disintegrants in a poorly water-soluble matrix has received much less attention. In this study, the disintegrants, crospovidone (XPVP), croscarmellose sodium (CCS) and sodium starch glycolate (SSG), were wet-granulated with dibasic calcium phosphate dihydrate as the poorly water-soluble matrix and polyvinylpyrrolidone as the binder. The effect of wet granulation was studied by evaluating tablet tensile strength and disintegratability. Comparison between tablets with granulated or ungranulated disintegrants as well those without disintegrants were also made. Different formulations showed different degrees of sensitivity to changes in tablet tensile strength and disintegratability post-wet granulation. Tablet tensile strength decreased for tablets with granulated disintegrant XPVP or CCS, but to a smaller extent for SSG. While tablets with granulated XPVP or CCS had increased disintegration time, the increment was lesser than for SSG, suggesting that wet granulation impacted a swelling disintegrant more. The findings showed that tablets with wet-granulated disintegrant had altered the disintegrant's functionality. These findings could provide better insights into changes in the disintegrant's functionality after wet granulation.
    MeSH terms: Chemistry, Pharmaceutical/methods; Drug Compounding/methods; Solubility*; Tablets*; Tensile Strength*; Drug Liberation
  15. Adisuri DS, Madhavan P, Chong PP, Nathan S
    J Pharm Sci, 2024 Jul 14.
    PMID: 39004416 DOI: 10.1016/j.xphs.2024.06.024
    Candidemia leaves a trail of approximately 750,000 cases yearly, with a morbidity rate of up to 30%. While Candida albicans still ranks as the most predominantly isolated Candida species, C. glabrata comes in second, with a death rate of 40-50%. Although infections by Candida spp are commonly treated with azoles, the side effects and rise in resistance against it has significantly limited its clinical usage. The current study aims to address the insolubility of piperine and provide an alternative treatment to Candida infection by formulating a stable piperine-loaded O/W nanoemulsion, comprised of Cremophor RH40, Transcutol HP and Capryol 90 as surfactant, co-surfactant, and oil, respectively. Characterization with zetasizer showed the droplet size, polydispersity (PDI) and zetapotential value of the nanoemulsion to be 24.37 nm, 0.453 and -21.10 mV, respectively, with no observable physical changes such as phase separation from thermostability tests. FTIR peaks confirms presence of piperine within the nanoemulsion and TEM imaging visualized the droplet shape and further confirms the droplet size range of 20-24 nm. The MIC90 value of the piperine-loaded nanoemulsion determined with in vitro broth microdilution assay was approximately 20-50% lower than that of the pure piperine in DMSO, at a range of 0.8-2.0 mg/mL across all Candida spp. tested. Overall, the study showed that piperine can be formulated into a stable nanoemulsion, which significantly enhances its antifungal activity compared to piperine in DMSO.
  16. Kurniawan TA, Mohyuddin A, Othman MHD, Goh HH, Zhang D, Anouzla A, et al.
    Water Environ Res, 2024 Jul;96(7):e11070.
    PMID: 39005104 DOI: 10.1002/wer.11070
    Every year, the global production of plastic waste reaches a staggering 400 million metric tons (Mt), precipitating adverse consequences for the environment, food safety, and biodiversity as it degrades into microplastics (MPs). The multifaceted nature of MP pollution, coupled with its intricate physiological impacts, underscores the pressing need for comprehensive policies and legislative frameworks. Such measures, alongside advancements in technology, hold promise in averting ecological catastrophe in the oceans. Mandated legislation represents a pivotal step towards restoring oceanic health and securing the well-being of the planet. This work offers an overview of the policy hurdles, legislative initiatives, and prospective strategies for addressing global pollution due to MP. Additionally, this work explores innovative approaches that yield fresh insights into combating plastic pollution across various sectors. Emphasizing the importance of a global plastics treaty, the article underscores its potential to galvanize collaborative efforts in mitigating MP pollution's deleterious effects on marine ecosystems. Successful implementation of such a treaty could revolutionize the plastics economy, steering it towards a circular, less polluting model operating within planetary boundaries. Failure to act decisively risks exacerbating the scourge of MP pollution and its attendant repercussions on both humanity and the environment. Central to this endeavor are the formulation, content, and execution of the treaty itself, which demand careful consideration. While recognizing that a global plastics treaty is not a panacea, it serves as a mechanism for enhancing plastics governance and elevating global ambitions towards achieving zero plastic pollution by 2040. Adopting a life cycle approach to plastic management allows for a nuanced understanding of possible trade-offs between environmental impact and economic growth, guiding the selection of optimal solutions with socio-economic implications in mind. By embracing a comprehensive strategy that integrates legislative measures and technological innovations, we can substantially reduce the influx of marine plastic litter at its sources, safeguarding the oceans for future generations.
    MeSH terms: Environmental Monitoring; Oceans and Seas*; Plastics; Water Pollutants, Chemical/analysis
  17. Tee PYE, Chu SYC, Kok CCY, Foo M, Tan CZJ, Foo JB, et al.
    Curr Pharm Biotechnol, 2024 Jul 12.
    PMID: 39005116 DOI: 10.2174/0113892010316710240626042205
    Clustered Regions of Interspersed Palindromic Repeat (CRISPR)-based techniques have been utilized in various research areas, including agriculture, biotechnology, and medicine. With the use of a short sequence guide RNA and CRISPR-associated (Cas) protein, this technique allows for robust, site-specific manipulation of the genome, aiding researchers in making important biomedical discoveries and scientific advancements. In this review, we explored the applications of CRISPR/Cas systems in the field of parasitology for the identification and validation of novel functional genes, diagnosis of parasitic infections, reduction of parasite virulence, and the disruption of disease transmission. We also discussed how CRISPR can be used for the development of therapeutics, vaccines, and drug discovery. Furthermore, the challenges and future perspectives of this technology are also highlighted.
  18. Ng MG, Tan HY, Ng PY, Koh RY, Voon KGL, Chye SM
    Curr Pharm Biotechnol, 2024 Jul 12.
    PMID: 39005118 DOI: 10.2174/0113892010307146240626080746
    BACKGROUND: Cancer is a significant issue worldwide. Generally, commercially available treatments, such as surgery, radiotherapy, and chemotherapy, are associated with undesirable complications. Hence, immunotherapy serves as a crucial alternative to those treatment options.

    OBJECTIVE: This modality is aimed to boost the immune system through the application of engineered antibodies, which can be produced using recombinant DNA technology.

    RESULTS: The discussion of the technologies leads to an introduction of the single-chain variable fragment (scFv). Thereafter, the advantages, disadvantages, and challenges associated with different expression systems, such as mammalian cells, yeast cells, bacterial cells, plant cells, and phage display were discussed comprehensively.

    CONCLUSION: Furthermore, conventional approaches such as hybridoma and modern approaches such as cell-free protein synthesis (CFPS) and simple colony assays are included. In short, this article has compiled evidence relating to each display system and may serve as a reference for those who aim to explore antibody engineering using one of the methods listed in this article.

  19. Fairlie T, Shah A, Wong RK, Fang X, Ghoshal UC, Kashyap PC, et al.
    PMID: 39005136 DOI: 10.1111/nmo.14866
    BACKGROUND: Disorders of gut-brain interaction (DGBI) are characterized by debilitating symptoms not explained by structural or biochemical abnormalities. While functional conditions present with complex, likely heterogeneous pathophysiology, we aimed to investigate if proxy measures of sociocultural and environmental factors are associated with the prevalence of various DGBI in populations across the world.

    METHODS: We performed an ecological study utilizing peer-reviewed published datasets reporting for 26 countries prevalence rates of DGBI (Rome Foundation Global Epidemiology Study, RFGES), with six independent variables: Helicobacter pylori prevalence and household size as proxy measures for orofecal infections, gross domestic product per capita (GDP), and median age as a proxy measures for socioeconomic development, density of fast food outlets (FFO) per 100,000 population as proxy measure for processed food exposure, and suicide mortality rate per 100,000 people, and world happiness scores were used as a proxy for psychological stress. The data were retrieved from publicly accessible datasets (United Nations, CIA World Factbook, World Bank, World Happiness Report, commercial/financial reports of a global FFO chain). We used linear regression to assess variables in univariate and multivariate analysis and report standardized β coefficients with 95% confidence intervals (CI).

    KEY RESULTS: The regression model revealed that the overall prevalence of DGBI was inversely associated with both GDP per capita (β = -0.57, 95% CI: -0.92, -0.22, p = 0.002) and happiness scores (β = -0.433 95% CI: 0.821, -0.065, p = 0.023), while being positively associated with H. pylori prevalence (β = 0.40, 95% CI: 0.008, 0.81, p = 0.046). The prevalence of functional constipation (FC) was also inversely associated with GDP per capita (β = -0.50, 95% CI: -0.86, -0.13, p = 0.01) and happiness scores (β = -0.497, 95% CI: -0.863, -0.132, p = 0.01), while being positively associated with H. pylori prevalence (β = 0.53, 95% CI: 0.16, 0.91, p = 0.007). The Multivariate model analysis revealed that combining the factors of H. pylori prevalence, suicide rate, household size and happiness scores showed statistically significant association with FC (p = 0.039). Household size (β = -0.43, 95% CI: -0.82, 0.038, p = 0.033) and suicide rates (β = 0.55, 95% CI: 0.19, 0.90, p = 0.004) were statistically significantly associated with functional diarrhea. Irritable bowel syndrome (IBS) was associated with GDP per capita (β = -0.40, 95% CI: -0.79, -0.014, p = 0.043) and happiness scores (β = -0.390, 95% CI: -0.778, -0.003, p = 0.049).

    CONCLUSIONS & INFERENCES: Utilizing publicly available data, the prevalence of DGBI across diverse countries is linked to various socio-cultural and environmental factors. Collectively, the data suggests that the prevalence of DGBI is increased in less prosperous regions of the world.

  20. Jadidoleslami A, Moshaverinia A, Moghaddas E, Singham GV
    J Arthropod Borne Dis, 2024 Mar;18(1):28-36.
    PMID: 39005538 DOI: 10.18502/jad.v18i1.15669
    BACKGROUND: The common bed bugs, Cimex lectularius, and tropical bed bugs, Cimex hemipterus are the primary species of public health importance in the family Cimicidae. This study aimed to determine the morphometric criteria and prevalent species of bed bugs in eastern Iran.

    METHODS: Bed bugs were collected from March 2021 to June 2022 from infested residential buildings and apartments in eastern Iran, including North Khorasan, Khorasan Razavi, and Sistan and Baluchistan Provinces. The morphological and morphometrical characteristics were used to identify collected bed bugs at inter- and intra-specific levels.

    RESULTS: A total of 34 isolates comprising 127 adult bed bugs were collected from Bojnord, Mashhad, Neishabur, Taibad, Sabzevar, Kashmer, Zahedan, Saravan, Rask, Pishin and Chabahar. Of these, 33 isolates (n=124) were found to be tropical bed bugs, C. hemipterus, and one population (n=3) was identified as C. lectularius. The index pronotal width/length ratio was calculated from 2.72 to 2.94 and 1.98 to 2.47 for C. lectularius and C. hemipterus, respectively. The length/width ratio of the hind femur was 3.365 in C. hemipterus and 4.267 in C. lectularius. The ratio of length/width of the third femur (F3 l/w) between populations of C. hemipterus was different, and this difference was statistically significant (P< 0.05).

    CONCLUSION: The results of this study indicated that C. hemipterus was the dominant bed bug species in the east of Iran and provided more morphometric criteria of C. hemipterus for researchers to identify the species and determine the intraspecific variations in the present and future.

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