Browse publications by year: 2024

  1. Wan Yusoff WSY, Abdul Zuki FA, Abu MN, Md Saad WM
    J Cytol, 2024;41(2):75-83.
    PMID: 38779605 DOI: 10.4103/joc.joc_110_23
    BACKGROUND AND OBJECTIVE: Trichomonas vaginalis is a causative agent of trichomoniasis, a sexually transmitted disease. In the cytology course context, students are taught to identify the cytomorphological characteristics of this organism. However, the limited learning period hinders their ability to recognize this infection effectively. This study intended to evaluate the efficacy of gamification by utilizing a web-based online game to enhance students' capacity to recognize Trichomonas vaginalis infection in cytology.

    MATERIALS AND METHODS: The study involved 50 Medical Laboratory Technology students who were randomly assigned to three groups. Group 1 (G1) participants received an interactive web-based online game called CytoUniverse, which comprised three components: a story-based game, a cytomorphology game, and a quiz focusing on Trichomonas vaginalis infection in cytology. Group 2 (G2) participants received the same information from a video lecture. Group 3 (G3) received both the web-based online game and the video lecture. The participants were assessed before the intervention (T1) and after the intervention (T2) to measure the effectiveness of the respective learning methods. IBM SPSS version 28 and GraphPad Prism version 9.0 were used to collect, tabulate, and analyze the data. By using descriptive analysis, the normality of the data was checked. Knowledge score and age were described as mean and standard deviation (SD) for numerical data. On the contrary, the categorical data, such as gender and group categories, were reported as frequencies and percentages. Fisher's exact test, paired t-test, and one-way ANOVA test were used in this study to determine the significance between groups.

    RESULTS: The study's results indicated a statistically significant improvement (P < 0.05) in knowledge scores at T2 compared to T1 for both G1 and G2 when compared to G3. However, there were no significant differences in knowledge scores between all groups for T1 or T2.

    CONCLUSIONS: In conclusion, gamification through a web-based online game may improve understanding of Trichomonas vaginalis infection. It looks to be a promising strategy for boosting students' knowledge and awareness to recognize Trichomonas vaginalis infection in cytology.

  2. Khan FM, Abbasi MA, Rehman AU, Siddiqui SZ, Sadiq Butt AR, Raza H, et al.
    RSC Adv, 2024 May 15;14(23):16546-16559.
    PMID: 38774615 DOI: 10.1039/d4ra01063a
    By using a convergent methodology, a unique series of N-arylated 4-yl-benzamides containing a bi-heterocyclic thiazole-triazole core was synthesized and the structures of these hybrid molecules, 9a-k, were corroborated through spectral analyses. The in vitro studies of these multi-functional molecules demonstrated their potent mushroom tyrosinase inhibition relative to the standard used. The kinetics mechanism was exposed by lineweaver-burk plots which revealed that, 9c, inhibited mushroom tyrosinase non-competitively by forming an enzyme-inhibitor complex. The inhibition constant Ki calculated from Dixon plots for this compound was 0.016 μM. The computational study was also consistent with the experimental results and these molecules disclosed good results of all scoring functions and interactions, which suggested a good binding to mushroom tyrosinase. So, it was predicted from the inferred results that these molecules might be considered as promising medicinal scaffolds for the diseases associated with the over-expression of this enzyme.
  3. Musa MA, Afandi AN, Zahedi FD
    Cureus, 2024 Apr;16(4):e58625.
    PMID: 38770504 DOI: 10.7759/cureus.58625
    The recurrence of nasopharyngeal carcinoma (NPC) is either at the local primary site or at regional or distant metastases. However, an axillary metastasis is a rare entity in NPC. We highlighted a case of recurrent NPC that presented with axillary swelling as the main initial complaint. Clinical examinations showed enlarged left axillary lymph nodes and left cervical lymph nodes. Histopathological examination of the axillary lymph node biopsy confirmed the recurrence of NPC. The patient underwent palliative chemotherapy in view of the advanced stage of recurrent disease. A thorough clinical history and examination during surveillance are crucial for early diagnosis and better survival outcomes.
  4. Rahim FAM, Salleh WMNHW, Ab Ghani N, Salihu AS, Arzmi MH
    Nat Prod Res, 2024 May 20.
    PMID: 38766974 DOI: 10.1080/14786419.2024.2353914
    This study was designed to investigate the chemical composition of the essential oil of Syzygium variolosum (King) Chantar. & J.Parn. and their cytotoxicity, acetylcholinesterase, antityrosinase, and anti-inflammatory activities. In total, 32 chemical components were identified in the essential oil, which made up 98.9%. The essential oil is mainly composed of β-elemene (20.2%), bicyclogermacrene (13.5%), viridiflorol (11.1%), globulol (8.6%), and selin-11-en-4α-ol (5.3%). Acetylcholinesterase, antityrosinase, and anti-inflammatory activities were evaluated with the Ellman method, mushroom tyrosinase, and lipoxygenase enzymes, respectively, while cytotoxicity was assessed using an MTT assay. The results showed that essential oil gave significant percentage inhibition (I%: acetylcholinesterase 35.2%, antityrosinase 42.5%, lipoxygenase 48.6%). Furthermore, the essential oil exhibited cytotoxicity against three cancer cell lines, HepG2, MCF7, and A549, with IC50 values ranging from 90.2 to 95.2 μg/mL. The current study highlights the potential of the use of essential oils as an alternative to the development of pharmaceutical antichemopreventives or cosmetics.
  5. Sharif-Nia H, She L, Allen KA, Marôco J, Kaur H, Arslan G, et al.
    BMC Public Health, 2024 May 18;24(1):1348.
    PMID: 38762744 DOI: 10.1186/s12889-024-18806-1
    AIM: Understanding vaccine hesitancy, as a critical concern for public health, cannot occur without the use of validated measures applicable and relevant to the samples they are assessing. The current study aimed to validate the Vaccine Hesitancy Scale (VHS) and to investigate the predictors of children's vaccine hesitancy among parents from Australia, China, Iran, and Turkey. To ensure the high quality of the present observational study the STROBE checklist was utilized.

    DESIGN: A cross-sectional study.

    METHOD: In total, 6,073 parent participants completed the web-based survey between 8 August 2021 and 1 October 2021. The content and construct validity of the Vaccine Hesitancy Scale was assessed. Cronbach's alpha and McDonald's omega were used to assess the scale's internal consistency, composite reliability (C.R.) and maximal reliability (MaxR) were used to assess the construct reliability. Multiple linear regression was used to predict parental vaccine hesitancy from gender, social media activity, and perceived financial well-being.

    RESULTS: The results found that the VHS had a two-factor structure (i.e., lack of confidence and risk) and a total of 9 items. The measure showed metric invariance across four very different countries/cultures, showed evidence of good reliability, and showed evidence of validity. As expected, analyses indicated that parental vaccine hesitancy was higher in people who identify as female, more affluent, and more active on social media.

    CONCLUSIONS: The present research marks one of the first studies to evaluate vaccine hesitancy in multiple countries that demonstrated VHS validity and reliability. Findings from this study have implications for future research examining vaccine hesitancy and vaccine-preventable diseases and community health nurses.

    MeSH terms: Adolescent; Adult; Australia; Child; China; Cross-Sectional Studies; Female; Humans; Iran; Male; Middle Aged; Psychometrics*; Surveys and Questionnaires; Turkey; Vaccination/psychology; Vaccination/statistics & numerical data; Reproducibility of Results
  6. Yean LE, Hashim SAB
    J Cardiothorac Surg, 2024 May 18;19(1):293.
    PMID: 38760859 DOI: 10.1186/s13019-024-02647-5
    BACKGROUND: Simulated self-practice using simulation models could improve fine motor skills and self confidence in surgical trainees.

    AIMS: The purpose of this study is to evaluate on self-reported confidence level in cardiothoracic surgical trainees by using surgical simulation models.

    METHODS: We conducted a cross-sectional study on all surgeons (n=10) involved in MIS simulation training. All surgeons are required to perform on three minimally invasive surgery (MIS) procedures (Mitral Valve Repair, Mitral Valve Replacement and Aortic Valve Replacement). A questionnaire was designed based on two existing scales related to self-confidence, the surgical self-efficacy scale [SSES] and the perceived competency scale [PCS]. We assessed their self-confidence (before and after training) in the use of simulation in MIS procedures using rating scales 1-5. The mean score was calculated for each domain and used as the predictor variable. We also developed six questions (PCS) using Objective Structured Assessment of Technical Skills (OSAT) related to each domain and asked participants how confident they were after performing each MICS procedure.

    RESULTS: The mean score was 4.7 for all assessed domains, except "knowledge" (3.8). Surgeons who had performed one or more MIS procedures had higher scores (P<0.05). There was no correlation between the number of MIS procedures performed and self-confidence scores.

    CONCLUSIONS: The results indicate that the cardiac surgery training based on MIS simulation improves trainees and consultants in terms of the level of self-confidence. Although surgeons generally have high levels of self-confidence after simulation training in MIS cardiac procedures, there is still room for improvement with respect to technical skills related to the procedure itself and its results.

  7. Ngadimon IW, Mohan D, Shaikh MF, Khoo CS, Tan HJ, Chamhuri NS, et al.
    Epilepsia, 2024 Jul;65(7):1962-1974.
    PMID: 38752783 DOI: 10.1111/epi.18007
    OBJECTIVE: Posttraumatic epilepsy (PTE) significantly impacts morbidity and mortality, yet local PTE data remain scarce. In addition, there is a lack of evidence on cognitive comorbidity in individuals with PTE in the literature. We sought to identify potential PTE predictors and evaluate cognitive comorbidity in patients with PTE.

    METHODS: A 2-year retrospective cohort study was employed, in which adults with a history of admission for traumatic brain injury (TBI) in 2019 and 2020 were contacted. Three hundred one individuals agreed to participate, with a median follow-up time of 30.75 months. The development of epilepsy was ascertained using a validated tool and confirmed by our neurologists during visits. Clinical psychologists assessed the patients' cognitive performance.

    RESULTS: The 2-year cumulative incidence of PTE was 9.3% (95% confidence interval [CI] 5.9-12.7). The significant predictors of PTE were identified as a previous history of brain injury [hazard ratio [HR] 4.025, p = .021], and intraparenchymal hemorrhage (HR: 2.291, p = .036), after adjusting for other confounders. TBI patients with PTE performed significantly worse on the total ACE-III cognitive test (73.5 vs 87.0, p = .018), CTMT (27.5 vs 33.0, p = .044), and PSI (74.0 vs 86.0, p = .006) than TBI patients without PTE. A significantly higher percentage of individuals in the PTE group had cognitive impairment, compared to the non-PTE group based on ACE-III (53.6% vs 46.4%, p = .001) and PSI (70% vs 31.7%, p = .005) scores at 2 years post-TBI follow-up.

    SIGNIFICANCE: This study emphasizes the link between TBI and PTE and the chance of developing cognitive impairment in the future. Clinicians can target interventions to prevent PTE by identifying specific predictors, which helps them make care decisions and develop therapies to improve patients' quality of life.

    MeSH terms: Adult; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Retrospective Studies; Risk Factors; Cohort Studies; Incidence; Young Adult
  8. Titisari N, Fauzi A, Abdul Razak IS, Mohd Noor MH, Samsulrizal N, Ahmad H
    Pharm Biol, 2024 Dec;62(1):447-455.
    PMID: 38753370 DOI: 10.1080/13880209.2024.2351933
    CONTEXT: Menhaden fish oil (FO) is widely recognized for inhibiting neuroinflammatory responses and preserving brain function. Nevertheless, the mechanisms of FO influencing brain cognitive function in diabetic states remain unclear.

    OBJECTIVE: This study examines the potential role of FO in suppressing LPS-induced neuroinflammation and cognitive impairment in diabetic animals (DA).

    MATERIALS AND METHODS: Thirty male Wistar rats were divided into 5 groups: i) DA received LPS induction (DA-LPS); ii) DA received LPS induction and 1 g/kg FO (DA-LPS-1FO); iii) DA received LPS induction and 3 g/kg FO (DA-LPS-3FO); iv) animals received normal saline and 3 g/kg FO (NS-3FO) and v) control animals received normal saline (CTRL). Y-maze test was used to measure cognitive performance, while brain samples were collected for inflammatory markers and morphological analysis.

    RESULTS: DA received LPS induction, and 1 or 3 g/kg FO significantly inhibited hyperglycaemia and brain inflammation, as evidenced by lowered levels of pro-inflammatory mediators. Additionally, both DA-LPS-1FO and DA-LPS-3FO groups exhibited a notable reduction in neuronal damage and glial cell migration compared to the other groups. These results were correlated with the increasing number of entries and time spent in the novel arm of the Y-maze test.

    DISCUSSION AND CONCLUSION: This study indicates that supplementation of menhaden FO inhibits the LPS signaling pathway and protects against neuroinflammation, consequently maintaining cognitive performance in diabetic animals. Thus, the current study suggested that fish oil may be effective as a supporting therapy option for diabetes to avoid diabetes-cognitive impairment.

    MeSH terms: Animals; Dose-Response Relationship, Drug; Lipopolysaccharides*; Male; Rats, Wistar*; Maze Learning/drug effects; Dietary Supplements*; Rats
  9. Dandy J, Ng Tseung-Wong C, George AM, Zamboanga BL, Palacios V
    PMID: 38753390 DOI: 10.1037/cdp0000659
    OBJECTIVES: Our aim was to identify the influence of heritage cultural factors and mainstream Australian cultural norms on young culturally and linguistically diverse (CaLD) immigrants' alcohol and other drug (AOD) use attitudes, motives for use, and behaviors.

    METHOD: We conducted nine focus groups with 55 youth (aged 16-30; 22 female, 33 male). Participants were from diverse cultural backgrounds including India, Sri Lanka, Ethiopia, Eritrea, Sudan, Hazara-Afghanistan, Burma (Chin and Karen), Malaysia, Singapore, and China. They were first- (80%) and second-generation immigrants to Australia.

    RESULTS: We constructed three overarching themes: bicultural conflict, freedom versus constraints, and the intersection of gender and culture. Although there were diverse responses, there was widespread recognition of perceived Australian norms around AOD use, particularly drinking alcohol. Our participants reported conformity motives that included fitting in with mainstream Australian culture. Drinking alcohol was also seen as a means for social bonding between immigrant and mainstream youth. Acculturation challenges of reconciling their heritage cultural identity with Australian identity contributed to problematic AOD use.

    CONCLUSIONS: Further research into the prevalence and predictors of AOD-related harm among CaLD youth in Australia is needed to tailor interventions that draw upon immigrant communities' resilience and enhance positive adaptation outcomes. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

  10. Johnson E, Sunil Kumar Sharma R, Ruiz Cuenca P, Byrne I, Salgado-Lynn M, Suraya Shahar Z, et al.
    Elife, 2024 May 16;12.
    PMID: 38753426 DOI: 10.7554/eLife.88616
    Zoonotic disease dynamics in wildlife hosts are rarely quantified at macroecological scales due to the lack of systematic surveys. Non-human primates (NHPs) host Plasmodium knowlesi, a zoonotic malaria of public health concern and the main barrier to malaria elimination in Southeast Asia. Understanding of regional P. knowlesi infection dynamics in wildlife is limited. Here, we systematically assemble reports of NHP P. knowlesi and investigate geographic determinants of prevalence in reservoir species. Meta-analysis of 6322 NHPs from 148 sites reveals that prevalence is heterogeneous across Southeast Asia, with low overall prevalence and high estimates for Malaysian Borneo. We find that regions exhibiting higher prevalence in NHPs overlap with human infection hotspots. In wildlife and humans, parasite transmission is linked to land conversion and fragmentation. By assembling remote sensing data and fitting statistical models to prevalence at multiple spatial scales, we identify novel relationships between P. knowlesi in NHPs and forest fragmentation. This suggests that higher prevalence may be contingent on habitat complexity, which would begin to explain observed geographic variation in parasite burden. These findings address critical gaps in understanding regional P. knowlesi epidemiology and indicate that prevalence in simian reservoirs may be a key spatial driver of human spillover risk.
    MeSH terms: Animals; Asia, Southeastern/epidemiology; Humans; Prevalence; Plasmodium knowlesi*; Ecosystem; Primate Diseases/epidemiology; Primate Diseases/parasitology; Primate Diseases/transmission
  11. Tay EA, Vijayakumar V, Morales RF, Lee ES, Teo A
    PLoS Pathog, 2024 May;20(5):e1012203.
    PMID: 38753622 DOI: 10.1371/journal.ppat.1012203
    MeSH terms: Endothelium, Vascular/metabolism; Endothelium, Vascular/virology; Humans; Endothelial Cells/metabolism; Endothelial Cells/virology
  12. Mubashir Hayat A, Abbas M, Emadifar H, Alzaidi ASM, Nazir T, Aini Abdullah F
    PLoS One, 2024;19(5):e0296909.
    PMID: 38753667 DOI: 10.1371/journal.pone.0296909
    The time fractional Schrödinger equation contributes to our understanding of complex quantum systems, anomalous diffusion processes, and the application of fractional calculus in physics and cubic B-spline is a versatile tool in numerical analysis and computer graphics. This paper introduces a numerical method for solving the time fractional Schrödinger equation using B-spline functions and the Atangana-Baleanu fractional derivative. The proposed method employs a finite difference scheme to discretize the fractional derivative in time, while a θ-weighted scheme is used to discretize the space directions. The efficiency of the method is demonstrated through numerical results, and error norms are examined at various values of the non-integer parameter, temporal directions, and spatial directions.
    MeSH terms: Algorithms*; Computer Simulation; Models, Theoretical; Quantum Theory
  13. Sutan R, Alavi K, Sallahuddin SN, Abdul Manaf MR, Jaafar MH, Shahar S, et al.
    PLoS One, 2024;19(5):e0302220.
    PMID: 38753828 DOI: 10.1371/journal.pone.0302220
    BACKGROUND: Community volunteering is defined as voluntary participation in activities and services to benefit the local community. It has potential benefits to promote social, physical, and mental well-being, and it enhances productive, healthy, and active aging. The tendency to volunteer varies across individuals and communities. There is limited knowledge of contributing factors influencing volunteering among Malaysian adults over the age of 50.

    AIMS: The present study aims to assess the association of demographic, cultural, and social factors with volunteering among Malaysian adults over the age of 50.

    METHODS: A cross-sectional study was conducted in 2020 involving 3,034 Malaysians aged 50 years and above across Malaysia, selected using a multi-stratified random sampling technique based on National Census 2020 data. A validated survey questionnaire to determine the demographic factor (age, sex, education level, employment status, health status, physical disability, and location of residence), cultural factor (ethnicity and religion), and social factor (social support, marital status, living arrangement, mode of transportation) that influence voluntary participation was distributed and collected. The association between these factors and volunteer participation was analysed using logistic regression models to identify significant predictors of voluntary participation among Malaysian adults over the age of 50.

    RESULTS: A regression model indicates that living in rural areas (OR 2.03, 95% CI 1.63-2.53), having higher education level (Tertiary level: OR 2.77, 95% CI 1.86-4.13), being employed (OR 1.31, 95% CI 1.10-1.56), differences in ethnicity background (Chinese: OR 0.58, 95% CI 0.39-0.86) and ease of transportation (Driving private transport: OR 1.26, 95% CI 1.19-1.32; Public transport: OR 1.07, 95% CI 1.00-1.154) were significantly associated with volunteering with R2 Nagelkerke of 0.147.

    CONCLUSION: Recognising various factors towards community volunteering should be addressed by policymakers and volunteer organisations to increase volunteer participation from potential adults over the age of 50 in promoting healthy and active ageing.

    MeSH terms: Aged; Cross-Sectional Studies; Female; Humans; Malaysia; Male; Middle Aged; Surveys and Questionnaires; Social Support
  14. Durrani N, Raziq A, Mahmood T, Khan MR
    PLoS One, 2024;19(5):e0298580.
    PMID: 38753854 DOI: 10.1371/journal.pone.0298580
    This study examines the antecedents of environmental sustainability in small and medium enterprises (SMEs) of a developing country and explores the specific internal and external factors for environmental sustainability. The study focused on SMEs in Balochistan, Pakistan, utilizing convenience and purposive sampling techniques to select a sample size of 30 SMEs. In-depth qualitative interviews were conducted using a semi-structured questionnaire. The results of the study revealed that lack of finance and education are major barriers to recognizing and addressing environmental sustainability issues, along with the lack of government support and regulations to ensure compliance with environmental safety laws, hence leading to low concern for sustainability practices among SMEs. Awareness and attitude of SME owners/managers, along with customer demand and government policies, influence the adoption of environmental sustainability practices. Overcoming financial constraints and promoting cooperation among stakeholders are key to fostering sustainable practices in SMEs. This research makes an important contribution to the sustainable management literature by providing new and in-depth insights into the barriers that impede environmental sustainability in SMEs of developing countries.
    MeSH terms: Developing Countries; Humans; Pakistan; Surveys and Questionnaires; Qualitative Research*
  15. Ankunda A, Ali Y, Mohanty M
    Accid Anal Prev, 2024 May 15;203:107633.
    PMID: 38754318 DOI: 10.1016/j.aap.2024.107633
    Facilitating proactive pedestrian safety management, the application of extreme value theory (EVT) models has gained popularity due to its extrapolation capabilities of estimating crashes from their precursors (i.e., conflicts). However, past studies either applied EVT models for crash risk analysis of autonomous vehicle-pedestrian interactions or human-driven vehicle-pedestrian interactions at signalised intersections. However, our understanding of human-driven vehicle-pedestrian interactions remains elusive because of scant evidence of (i) EVT models' application for heterogeneous traffic conditions, (ii) appropriate set of determinants, (iii) which EVT approach to be used, and (iv) which conflict measure is appropriate. Addressing these issues, the objective of this study is to investigate pedestrian crash risk analysis in heterogeneous and disordered traffic conditions, where drivers do not follow lane disciplines. Eleven-hour video recording was collected from a busy pedestrian crossing at a midblock location in India and processed using artificial intelligence techniques. Vehicle-pedestrian interactions are characterised by two conflict measures (i.e., post encroachment time and gap time) and modelled using block maxima and peak over threshold approaches. To handle the non-stationarity of pedestrian conflict extremes, several explanatory variables are included in the models, which are estimated using the maximum likelihood estimation procedure. Modelling results indicate that the EVT models provide reasonable estimates of historical crash records at the study location. From the EVT models, a few key insights related to vehicle-pedestrian interactions are as follows. Firstly, a comparison of EVT models shows that the peak over threshold model outperforms the block maxima model. Secondly, post encroachment time conflict measure is found to be appropriate for modelling vehicle-pedestrian interactions compared to gap time. Thirdly, pedestrian crash risk significantly increases when they interact with two-wheelers in contrast with interactions involving buses where the crash risk decreases. Fourthly, pedestrian crash risk decreases when they cross in groups compared to crossing individually. Finally, pedestrian crash risk is positively related to average vehicle speed, pedestrian speed, and five-minute post encroachment time counts less than 1.5 s. Further, different block sizes are tested for the block maxima model, and the five-minute block size yields the most accurate and precise pedestrian crash estimates. These findings demonstrate the applicability of extreme value analysis for heterogeneous and disordered traffic conditions, thereby facilitating proactive safety management in disordered and undisciplined lane conditions.
  16. Ramli NA, Adam F, Ries ME, Ibrahim SF
    Int J Biol Macromol, 2024 May 14.
    PMID: 38754668 DOI: 10.1016/j.ijbiomac.2024.132385
    CNCs are intensively studied to reinforce biocomposites. However, it remains a challenge to homogeneously disperse the CNC in biocomposites for a smooth film surface. Mechanochemical treatment via ultrasonication in deep eutectic solvent (DES) generated a stable dispersion of CNC before incorporation into carrageenan biocomposite. Shifted peaks of choline chloride (ChCl) methylene groups to 3.95-3.98 ppm in 1H NMR indicated a formation of eutectic mixture between the hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD) at the functional group of CH3···OH. The swelling of CNC in the DES was proven by the formation of intermolecular H-bond at a length of 2.46 Å. The use of DES contributed to a good dispersion of CNC in the solution which increased zeta potential by 43.2 % compared to CNC in deionized water. The ultrasonication amplitude and feed concentration were varied for the best parameters of a stable dispersion of CNC. The crystallinity of 1 wt% of CNC at 20 % sonication amplitude improved from 76 to 81 %. The high crystallinity of CNCDES resulted in an increase in film tensile and capsule loop strength of Carra-CNCDES by 20.7 and 19.4 %, respectively. Improved dispersion of CNCDES reduced the surface roughness of the biocomposite by 21.8 %. H-bond network in CNCDES improved the biocomposite properties for an ingenious reinforcement material.
  17. Kumar P, Abubakar AA, Ahmed MA, Hayat MN, Halim FA, Rahman MM, et al.
    Anim Biosci, 2024 May 07.
    PMID: 38754849 DOI: 10.5713/ab.24.0050
    OBJECTIVE: The livestock handler attitude and their handling of animals is crucial for improving animal welfare standards, minimizing stress, improving productivity and meat quality. The present study was undertaken to assess the effect of training livestock handlers on behavioral, physiological, and hormonal responses during preslaughter handling in goats.

    METHODS: A total of 6 handlers were divided into trained (trained in basic animal handling practices, animal behavior, and animal welfare), contact trained (not trained directly but interacted and saw the working of trained handlers), and untrained groups (no formal training). The handling experiment was conducted on 18 male goats by following a cross-over design. The goats were moved from lairage to slaughter point by trained, contact-trained, and untrained handlers. Various behavioral, physiological, and hormonal parameters were recorded at the lairage before handling and at the slaughter point after handling the goats.

    RESULTS: The training of livestock handlers had a significant effect on behavioral, physiological, and hormonal responses in goats. The goats handled by untrained and contact-trained handlers were recorded with intense vocalization, significant (p<0.05) increase in heart rate and blood glucose, and catecholamines (adrenaline and nor-adrenaline), thereby indicating stress and poor animal welfare. The trained handlers were observed to use visual interactions (waving of hands or objects, blocking, hand raising, etc), and lower stress responses were recorded in the goats handled by this group.

    CONCLUSION: The present study highlights the importance of training to livestock handlers in improving animal welfare and minimizing stress in goats during preslaughter stress.

  18. Balqis-Ali NZ, Fun WH
    BMJ Open, 2024 May 15;14(5):e077046.
    PMID: 38754882 DOI: 10.1136/bmjopen-2023-077046
    OBJECTIVE: This study aimed to examine the mediation role of perceived social support in the relationship between functional limitations, depressive symptoms and quality of life among older people in Malaysia.

    SETTING: The Malaysian National Health Morbidity Survey 2018: Elderly Health was a cross-sectional health community survey among adults aged 50 and above.

    PARTICIPANTS: 3977 community-dwelling older Malaysians aged 60 and above.

    OUTCOME MEASURES: Functional limitations were defined as personal activities of daily living (PADL) and instrumental activities of daily living (IADL), tested in separate paths in all analyses. PADL was measured using the Barthel Index, while IADL was measured using the Lawton and Brody scale. Perceived social support, depressive symptoms and quality of life were measured using the Duke Social Support Index, Geriatric Depression Scale-14 and Control, Autonomy, Self-Realisation and Pleasure-19 tools. We used mediation analysis through structural equation modelling to explore the role of perceived social support.

    RESULTS: Perceived social support mediated the relationship between PADL and IADL with depressive symptoms, with the indirect effects at -0.079 and -0.103, respectively (p<0.001). Similarly, perceived social support mediated the relationship between PADL and IADL with quality of life, with the indirect effects at 0.238 and 0.301, respectively (p<0.001). We performed serial multiple mediation analysis and found that perceived social support and depressive symptoms mediated the path between PADL and IADL with quality of life, with the indirect effects at 0.598 and 0.747, respectively (p<0.001). The relationship between functional limitations and all outcomes remained significant in all mediation analyses.

    CONCLUSION: The present study provides evidence that perceived social support relieves the influence of functional limitations on depressive symptoms and declining quality of life among older people. Therefore, it is imperative to establish a social support system to improve the overall well-being of older people.

    MeSH terms: Activities of Daily Living*; Aged; Aged, 80 and over; Cross-Sectional Studies; Female; Humans; Malaysia/epidemiology; Male; Mental Health*; Middle Aged; Quality of Life*; Social Support*; Geriatric Assessment/methods
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