Affiliations 

  • 1 Center for Neuroscience Research, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Jalan Hospital, Sungai Buloh, Selangor Darul Ehsan, Selangor, Malaysia; Universiti Teknologi MARA, RIG "Molecular Pharmacology and Advanced Therapeutics," Pharmaceutical & Life Sciences (PLS) Communities of Research (CoRe), Shah Alam, Selangor Darul Ehsan, Malaysia; Volgograd State Medical University, Research Institute of Pharmacology, 1 Pavshikh Bortsov sq., Volgograd, Russian Federation
  • 2 Center for Neuroscience Research, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Jalan Hospital, Sungai Buloh, Selangor Darul Ehsan, Selangor, Malaysia; Universiti Teknologi MARA, RIG "Molecular Pharmacology and Advanced Therapeutics," Pharmaceutical & Life Sciences (PLS) Communities of Research (CoRe), Shah Alam, Selangor Darul Ehsan, Malaysia
  • 3 Center for Neuroscience Research, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Jalan Hospital, Sungai Buloh, Selangor Darul Ehsan, Selangor, Malaysia
  • 4 International Medical University, IMU Clinical School, Department of Ophthalmology, Jalan Rasah, Seremban, Malaysia
  • 5 Volgograd State Medical University, Research Institute of Pharmacology, 1 Pavshikh Bortsov sq., Volgograd, Russian Federation
  • 6 Scientific Centre for Expertise of Medical Application Products, Ministry of Health, Russian Federation
Mol. Vis., 2016;22:734-47.
PMID: 27440992

Abstract

PURPOSE: Increased lenticular oxidative stress and altered calcium/magnesium (Ca/Mg) homeostasis underlie cataractogenesis. We developed a liposomal formulation of magnesium taurate (MgT) and studied its effects on Ca/Mg homeostasis and lenticular oxidative and nitrosative stress in galactose-fed rats.

METHODS: The galactose-fed rats were topically treated with liposomal MgT (LMgT), liposomal taurine (LTau), or corresponding vehicles twice daily for 28 days with weekly anterior segment imaging. At the end of the experimental period, the lenses were removed and subjected to analysis for oxidative and nitrosative stress, Ca and Mg levels, ATP content, Ca(2+)-ATPase, Na(+),K(+)-ATPase, and calpain II activities.

RESULTS: The LTau and LMgT groups showed significantly lower opacity index values at all time points compared to the corresponding vehicle groups (p<0.001). However, the opacity index in the LMgT group was lower than that in the LTau group (p<0.05). Significantly reduced oxidative and nitrosative stress was observed in the LTau and LMgT groups. The lens Ca/Mg ratio in LMgT group was decreased by 1.15 times compared to that in the LVh group. Calpain II activity in the LMgT group was decreased by 13% compared to the LVh group. The ATP level and Na(+),K(+)-ATPase and Ca(2+)-ATPase activities were significantly increased in the LMgT group compared to the LVh group (p<0.05).

CONCLUSIONS: Topical liposomal MgT delays cataractogenesis in galactose-fed rats by maintaining the lens mineral homeostasis and reducing lenticular oxidative and nitrosative stress.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.