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Fabrication and Characterization of an Electrospun PHA/Graphene Silver Nanocomposite Scaffold for Antibacterial Applications

Mukheem A 1 , Muthoosamy K 2 , Manickam S 3 , Sudesh K 4 , Shahabuddin S 5 , Saidur R 6 Show all authors , Akbar N 7 , Sridewi N 8

Affiliations 

  • 1 Department of Maritime Science and Technology Faculty of Science and Defence Technology, National Defence University of Malaysia, Kuala Lumpur 57000, Malaysia. mukheembio@gmail.com
  • 2 Department of Chemical and Nano pharmaceutical Process Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Semenyih 43500, Malaysia. Kasturi.Muthoosamy@nottingham.edu.my
  • 3 Department of Chemical and Nano pharmaceutical Process Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Semenyih 43500, Malaysia. sivakumar.manickam@nottingham.edu.my
  • 4 Applied Microbiology and Ecobiomaterial Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia. ksudesh@usm.my
  • 5 Research Centre for Nano-Materials and Energy Technology (RCNMET), School of Science and Technology, Sunway University, Subang Jaya 47500, Malaysia. syedshahab.hyd@gmail.com
  • 6 Research Centre for Nano-Materials and Energy Technology (RCNMET), School of Science and Technology, Sunway University, Subang Jaya 47500, Malaysia. saidur@sunway.edu.my
  • 7 Department of Biological Sciences, School of Science and Technology, Sunway University, Subang Jaya 47500, Malaysia. noormicrobiologist555@gmail.com
  • 8 Department of Maritime Science and Technology Faculty of Science and Defence Technology, National Defence University of Malaysia, Kuala Lumpur 57000, Malaysia. nanthini@upnm.edu.my
Materials (Basel), 2018 Sep 10;11(9).
PMID: 30201852 DOI: 10.3390/ma11091673

Abstract

Many wounds are unresponsive to currently available treatment techniques and therefore there is an immense need to explore suitable materials, including biomaterials, which could be considered as the crucial factor to accelerate the healing cascade. In this study, we fabricated polyhydroxyalkanoate-based antibacterial mats via an electrospinning technique. One-pot green synthesized graphene-decorated silver nanoparticles (GAg) were incorporated into the fibres of poly-3 hydroxybutarate-co-12 mol.% hydroxyhexanoate (P3HB-co-12 mol.% HHx), a co-polymer of the polyhydroxyalkanoate (PHA) family which is highly biocompatible, biodegradable, and flexible in nature. The synthesized PHA/GAg biomaterial has been characterized by field emission scanning electron microscopy (FESEM), elemental mapping, thermogravimetric analysis (TGA), UV-visible spectroscopy (UV-vis), and Fourier transform infrared spectroscopy (FTIR). An in vitro antibacterial analysis was performed to investigate the efficacy of PHA/GAg against gram-positive Staphylococcus aureus (S. aureus) strain 12,600 ATCC and gram-negative Escherichia coli (E. coli) strain 8739 ATCC. The results indicated that the PHA/GAg demonstrated significant reduction of S. aureus and E. coli as compared to bare PHA or PHA- reduced graphene oxide (rGO) in 2 h of time. The p value (p < 0.05) was obtained by using a two-sample t-test distribution.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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