Affiliations 

  • 1 School of Pharmacy, Monash University, Jalan Lagoon Selatan, 47500, Bandar Sunway, Selangor Darul Ehsan, Malaysia; Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, Pakistan. Electronic address: hammad.saleem@monash.edu
  • 2 Department of Biology, Faculty of Science, Selcuk University, Campus/Konya, Turkey. Electronic address: gokhanzengin@selcuk.edu.tr
  • 3 Department of Pharmacy, University 'G. d'Annunzio" of Chieti-Pescara, 66100, Chieti, Italy
  • 4 Department of Pharmacy, The Islamia University of Bahawalpur, Pakistan
  • 5 Department of Health Sciences, Faculty of Science, University of Mauritius, Mauritius
  • 6 University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, Pakistan
  • 7 Department of Bioresources and Food Science, Konkuk University, Seoul, 05029, South Korea
  • 8 Liquid Chromatography Mass Spectrometery (LCMS) Platform, Monash University, Jalan Lagoon Selatan, 47500, Bandar Sunway, Selangor, Darul Ehsan, Malaysia
  • 9 School of Pharmacy, Monash University, Jalan Lagoon Selatan, 47500, Bandar Sunway, Selangor Darul Ehsan, Malaysia; Tropical Medicine and Biology Multidisciplinary Platform, Monash University Malaysia, Jalan Lagoon Selatan, 47500, Bandar Sunway, Selangor, Darul Ehsan, Malaysia. Electronic address: nafees.ahemad@monash.edu
Food Chem. Toxicol., 2019 Sep;131:110535.
PMID: 31154083 DOI: 10.1016/j.fct.2019.05.043

Abstract

This study endeavours to investigate the phytochemical composition, biological properties and in vivo toxicity of methanol and dichloromethane extracts of Zaleya pentandra (L.) Jeffrey. Total bioactive contents, antioxidant (phosphomolybdenum and metal chelating, DPPH, ABTS, FRAP and CUPRAC) and enzyme inhibition (cholinesterases, tyrosinase α-amylase, and α-glucosidase) potential were assessed utilizing in vitro bioassays. UHPLC-MS phytochemical profiling was carried out to identify the essential compounds. The methanol extract was found to contain highest phenolic (22.60 mg GAE/g) and flavonoid (31.49 mg QE/g) contents which correlate with its most significant radical scavenging, reducing potential and tyrosinase inhibition. The dichloromethane extract was most potent for phosphomolybdenum, ferrous chelation, α-amylase, α-glucosidase, and cholinesterase inhibition assays. UHPLC-MS analysis of methanol extract unveiled to identify 11 secondary metabolites belonging to five sub-groups, i.e., phenolic, alkaloid, carbohydrate, terpenoid, and fatty acid derivatives. Additionally, in vivo toxicity was conducted for 21 days and the methanol extract at different doses (150, 200, 250 and 300 mg/kg) was administered in experimental chicks divided into five groups each containing five individuals. Different physical, haematological and biochemical parameters along with the absolute and relative weight of visceral body organs were studied. Overall, no toxic effect was noted for the extract at tested doses.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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