Staphylococcus kloosii, an orange pigment-producing bacterium, was isolated from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) from Teluk Nipah, Pangkor Island, Perak, Malaysia. This report is the first documentation of this Gram-positive strain, referred to as Strain 68 in Malaysia. A partial 16S ribosomal RNA gene sequence of the mesophilic strain has been registered with GenBank (National Center for Biotechnology Information, US National Library of Medicine) with accession number JX102547. Phylogenetic analysis using the neighbour-joining method further supported the identification of Strain 68 as S. kloosii. The circular strain produced orange pigments on tryptone glucose yeast extract agar (TGYEA) and in nutrient broth (NB) at approximately pH 7. The visible spectra of ethanolic and methanolic pigment extracts of the bacterial strain were considered identical with λmax at 426, 447 and 475 nm and λmax at 426, 445 and 473 nm, respectively. Both visible spectra resemble the visible spectra of lutein, which is a commercial carotenoid; however, further analyses are required to confirm the identity of this pigment. The methanolic extracts of the intracellular pigments comprised at least three pigment compounds: an orange pigment compound (major compound), a yellow pigment compound (the least polar) and a pink pigment compound (the most polar). These findings are the first documentation of the pigment composition of S. kloosii as no such record could be found to date.
Maritime transportation is very important for coastal state country like Malaysia. However, as having the busiest straits in the world, Malaysia is continuously exposed to the risk of marine spill. The nation had experienced around 30 marine spill incidents since 1976 to the present. The main contributor of marine spill is ship’s accident and in term of category of accident, collision had contributed the most. In term of type of substance that mostly spilled by ships is highly persistence crude oil. The management of marine spill response of Malaysia is applying the three tiers system which is base on the area and scale of the spill. The basis of establishing the marine response service is in accordance with OPRC, which incorporated into the national environmental law. With the three tiers of response system, Malaysia is equipped with arsenal of marine spill response and control equipments. However, with the present magnitude of threat, the current capacities of equipments are insufficient. Nevertheless, the possibility of full magnitude of marine spill is unlikely due to the safety features incorporated into the design of the ships and FSO/FPSO. In term of claim and compensation for marine spill incident, Malaysia is applying the two layers of compensation under the CLC 1992 Protocol and FUND 1992 Protocol. Therefore, Malaysia is ready in various aspects of marine spill response and control.
This study on anthropometrics of Primary School children from grade 1 to 5 in Peninsular Malaysia involves 2310 students aged seven to eleven years old. The objectives were to analyze the differentiation of anthropometrics between children of grades 1 to 5 and grouping them to suitable levels in which they are appropriate to propose chair dimensions. A multi-stage sampling method was used, and rural and urban areas were also included in providing anthropometric database that represents the whole Peninsular Malaysia population. There were six dimensions measured in this study, which are sitting shoulder, sitting subscapular height, sitting elbow height, hip width, buttock-popliteal length and popliteal height. All the measurements were chosen to represent dimensions needed to construct ergonomic school chair. From the results, ANOVA showed p-values of
Objective: The study aims to determine pattern of verbal memory and learning impairment and its associated factors among patients with bipolar I disorder in a psychiatric clinic of a university hospital. Methods: A case control study comparing verbal memory test
performance in 40 patients with bipolar I disorder to that of 40 healthy normal subjects using Rey Auditory Verbal Learning Test (RAVLT). The association between demographic, clinical
characteristics and poor verbal memory performance were examined. Results: Up to 92% of patients with bipolar I disorder have impaired short term working memory in this hospital-based study. They also recalled fewer words in all the RAVLT trials and had difficulties
learning the word list in comparison to that of normal healthy individuals. Verbal memory and learning impairment are observed in bipolar illness in the absence of active mood symptoms while duration and severity of illness are not found to have any effect on verbal memory and learning. Conclusion: There is consistent verbal memory and learning problems in individuals with bipolar I disorder and their presence in the absence of mania, depression and mixed symptoms during the course of the illness suggests a trait related deficit.
Study site: Psychiatric Clinic, Pusat Perubatan Universiti Kebangsaan Malaysia (PPUKM)
Urografi intravena (IVU) dan tomografi berkomputer helikal tanpa kontras (UHCT) urografi adalah dua prosedur utama yang akan dijalankan semasa penyiasatan radiologi bagi pengesanan urolitiasis (batu karang) pada sistem genitourinari. Dedahan terhadap sinaran radiasi merupakan faktor kebimbangan utama dalam kedua-dua prosedur. Oleh itu, satu kajian perbandingan dos sinaran telah dijalankan antara prosedur IVU dan UHCT urografi di samping menentukan faktor dedahan optimum bagi kedua-dua prosedur tersebut. Kajian ini telah dijalankan ke atas fantom antropomorfik seluruh tubuh mengikut protokol sebenar bagi prosedur UHCT urografi dan penghasilan radiografi bersiri berserta dengan pemberian media berkontras bagi prosedur IVU. Sebanyak tiga parameter dedahan voltan tiub digunakan iaitu 75 kVp, 80 kVp dan 85 kVp bagi prosedur IVU dan 100 kVp, 120 kVp dan 140 kVp bagi prosedur UHCT urografi . Hasil dos sinaran bagi prosedur IVU yang diperolehi adalah 1.40 mSv, 2.10 mSv dan 2.79 mSv bagi 75 kVp, 80 kVp dan 85 kVp. Manakala bagi prosedur UHCT urografi , sebanyak 0.76 mSv, 1.32 mSv dan 1.82 mSv dos sinaran direkodkan bagi 100 kVp, 120 kVp dan 140 kVp. Hasil kualiti imej optimum adalah menggunakan dedahan sebanyak 85 kVp bagi prosedur IVU dan 120 kVp bagi prosedur UHCT urografi . Kesimpulannya, walaupun tidak terdapat perbezaan signifi kan, dos sinaran yang terhasil daripada prosedur IVU adalah kekal lebih tinggi daripada prosedur UHCT urografi .
The objective of this study is to determine the prevalence and factors associated with psychosis in Parkinson’s disease (PD) patients.
Method: This is a cross-sectional study of 108 PD patients from neurological clinic UKM Medical Centre and Kuala Lumpur Hospital. The patients were recruited from August to December 2004. Psychosis was determined using SCID, the severity of psychosis was rated using BPRS. The cognitive functions were evaluated with MMSE and the severity of depression was assessed with HAM-D.
Results: The result shows that the prevalence of psychosis in Parkinson's disease patients in this sample was 13%. The psychosis was found to be significantly associated with advancing age, duration of illness, severity of depression and cognitive impairment. Multivariate analysis demonstrates that severity of depression (OR = 1.08, 95% C.I. = 1.01 - 1.16) and advancing age (OR = 4.72, 95% C.I. = 1.37 - 16.29) increased risk of psychosis in PD patients.
Conclusion: We found that advancing age and severity of depression increase risk to develop psychosis in patients with PD.
Study site: neurological clinic UKM Medical Centre and Kuala Lumpur Hospita
In this study, functional magnetic resonance imaging (fMRI) is used to investigate func-tional specialisation in human auditory cortices during listening. A silent fMRI paradigm was used to reduce the scanner sound artefacts on functional images. The subject was instructed to pay attention to the white noise stimulus binaurally given at an inten-sity level of 70 dB higher than the hearing level for normal people. Functional speciali-sation was studied using the Matlab-based Statistical Parametric Mapping (SPM5) software by means of fixed effects (FFX), random effects (RFX) and conjunction analyses. Individual analyses on all subjects indicated asymmetrical bilateral activation of the left and right hemispheres in Brodmann areas (BA) 22, 41 and 42, involving the primary and secondary auditory cortices. The percentage of signal change is larger in the BA22, 41 and 42 on the right as compared to the ones on the left (p>0.05). The average number of activated voxels in all the respective Brodmann areas are higher in the right hemisphere than in the left (p>0.05). FFX results showed that the point of maximum intensity was in the right BA41 whereby 599±1 activated voxels were ob-served in the right temporal lobe as compared to 485±1 in the left temporal lobe. The RFX results were consistent with that of FFX. The analysis of conjunction which fol-lowed, showed that the right BA41 and left BA22 as the common activated areas in all subjects. The results confirmed the specialisation of the right auditory cortices in pro-cessing non verbal stimuli.
The Indian ethnic group remains underrepresented despite the continued advancement in the field of speech and language
therapy (SLT) in Malaysia. This study aims to investigate the perception of Malaysian Indian Pre-university students towards
a career in the health sciences and specifically for the SLT profession. This was a cross-sectional study that employed
convinience sampling method to recruit participants. A total of 110 Indian pre-university students from urban cities of
Kuala Lumpur and Johor were recruited. The findings showed majority of the respondents (>90%) placed importance
on a career that provided “high salary,” “job stability,” and “career’s social standing in the community.” Almost all
respondents recognize the medical, pharmacy and nursing profesion compared to only
Biocompatibility and growth of osteoblast on bone scaffolds play an important role towards their therapeutic application.
The presence of oxidative stress generated by bone scaffolds highly influences osteoblast growth and its functional
performance. In this study in-vitro interaction of developed Alginate/Cockle Shell powder nanobiocomposite bone scaffold
on osteoblast with regards to cytotoxicity and oxidative stress are evaluated. Cytotoxicity studies using MTT assays
revealed a significant increase in viability of cultured osteoblast in the presences of the scaffold extracts. The growth of
osteoblast on the scaffold were not deterred with the presence of any major oxidative stress factors as determined through
oxidative stress profile studies using SOD, GSH and ROS assays. The nanobiocomposite scaffold evaluated in this study
shows promising use in regards to facilitating osteoblast proliferation, growth and viability.
The mismatch in coefficient of thermal expansion (CTE) between the veneered
porcelain and the ceramic core has been primarily identified as the cause of core/veneer chipping
in all-ceramic restorations. This study aimed to evaluate the effect of sodalite infiltration on the
CTE behaviour and bond strength of different all-ceramic prostheses. Materials and Methods: The
experimental groups were synthesised sodalite-infiltrated alumina (IA-SOD) and synthesised
sodalite-infiltrated zirconia-toughened alumina (ZTA) (IZ-SOD), while the control groups were glassinfiltrated alumina (IA-glass) and glass-infiltrated ZTA (IZ-glass). (Copied from article).
The aims of this study are (1) to determine the scattered radiation dose levels in routine fluoroscopy procedures and (2) to compare them with the equivalent chest x-rays and also (3) to monitor common techniques and radiation safety measures taken by the medical officers. The study covered a sample of 105 fluoroscopic procedures performed by 18
medical officers. Each officer wore a personal pocket dosimeter inside the lead gown during each procedure. A digital dosimeter was placed near the detector of the fluoroscopy unit while a survey meter was positioned at the control panel area to record the dose levels. There were 14 types of examination included in this study. The total number of images
captured was found to be the highest in barium swallow examination with 115 images, almost five times higher compared to the common practices. The longest screening time was observed in barium enema examination which is 9.15 seconds. The median of the scattered dose level was the highest in barium meal examination (165.50 µSv) which is equivalent
to 8.28 times of average dose impart by chest x-ray examinations. The number of images and the length of screening time depend on the competency levels of the medical officers. They capture as many images as possible to avoid missing any abnormalities, therefore it will always be better if the fluoroscopist is consulted during each case. They should also
consistently practice essential protection by minimizing exposure time, maximizing distance from the source tube and utilizing the radiation shielding.
The aim of this study is to predict the next day PM10 concentration using Bayesian Regression with noninformative
prior and conjugate prior models. The descriptive analysis of PM10, temperature, relative humidity,
nitrogen dioxide (NO2), sulphur dioxide (SO2), carbon monoxide (CO) and ozone (O3) are also included. A case
study used two-years of air quality monitoring data at three (3) monitoring stations to predict the future PM10
concentration with seven parameters (PM10, temperature, relative humidity, NO2, SO2, CO, and O3). The descriptive
analysis showed that the highest mean PM10 concentration occurred at Klang station in 2011 (71.30 µg/m3
) followed
by 2012 (68.82 µg/m3
). The highest mean PM10 concentration was at Nilai in 2012 (68.86 µg/m3
) followed by 2011
(66.29µg/m3
) respectively. The results showed that the Bayesian regression model used a conjugate prior with a
normal-gamma prior which was a good model to predict the PM10 concentration for most study stations with (R2 =
0.67 at Jerantut station), (R2 = 0.61 at Nilai station) and (R2 = 0.66 at Klang station) respectively compared to a
non-informative prior.
Penghasilan CGTase daripada Bacillus sp. G1 berjaya ditingkatkan dengan menggunakan sistem kultur selanjar mengatasi penghasilan daripada kultur kelompok. Aktiviti CGTase tertinggi yang didapati dalam kultur kelompok ialah 28.1 U/ml. Kajian kultur selanjar difokuskan kepada masa kemasukan medium segar yang berbeza (12, 24 dan 48 jam ), kadar pencairan ditetapkan pada 0.03 per jam. Hasil menunjukkan masa memulakan pam medium segar tidak memberi perubahan yang signifikan terhadap aktiviti CGTase (25.7, 26.3 dan 26.1 U/ml masing-masing) dan produktiviti CGTase (0.77, 0.79 dan 0.78 U/ml/j masing-masing) pada keadaan mantap tetapi produktiviti CGTase (0.77 U/ml/j) akan lebih tinggi berbanding produktiviti kultur kelompok apabila masa di antara larian kultur kelompok diambil kira. Malah peningkatan berpotensi ditingkatkan lagi dengan memulakan pam medium segar lebih awal daripada 12 jam dan juga dengan meningkatkan kadar pencairan.
The microstructure and phase formation of Au-In thin film deposited by e-beam evaporation technique has been studied. Single crystals of rocksalt were used as the substrates. The chamber pressure during deposition was about 2.5 × 10-5 torr and substrate temperature was 35°C. Three types of samples were prepared namely Au, In and Au-In thin films. Microstructure and chemical composition of these thin films were characterized by transmission electron microscopy (TEM) and X-ray photoelectron spectrometer (XPS) respectively. TEM micrograph reveals island structures for both Au and In thin film on the rocksalt substrate, with the In island size distribution is about 9-30 nm compared to Au island in the range of 3-10 nm. The growth of islands instead of smooth film indicates that Au and In thin films follow the Volmer-Weber growth mode. However, island structures were not present on Au-In thin films which most probably follow the Frank van de Merwe growth mode. XPS analysis indicates intermetallic compound was not present in the Au-In thin film suggesting that diffusion process in the interface of Au and In films is minimal.
Mekanisme pembentukan apatit pada permukaan β-wolastonit dikaji. β-wolastonit dihasilkan daripada teknik sol-gel menggunakan abu sekam dan batu kapur terkalsin sebagai bahan pemula dengan nisbah CaO:SiO2 adalah 55:45. Kebioaktifan sampel β-wolastonit dikaji dengan merendam sampel berbentuk silinder dalam larutan simulasi badan (SBF) untuk tempoh yang ditetapkan iaitu 1, 3, 7 dan 14 hari. Komposisi permukaan, morfologi dan perubahan struktur sampel sebelum dan selepas direndam dianalisis melalui pembelauan sinar-X (XRD) dan mikroskop elektron imbasan (FESEM) yang digabungkan dengan EDX. Keputusan XRD menunjukkan fasa β-wolastonit berjaya dihasilkan selepas dimasukkan ke dalam autoklaf untuk 8 jam pada suhu 135°C pada tekanan 0.24 MPa dan disinter 2 jam pada suhu 950°C. Apatit didapati tumbuh pada permukaan sampel β-wolastonit selepas 7 hari rendaman dalam larutan SBF. Semasa proses rendaman dalam larutan SBF, 2 jenis kumpulan kalsium fosfat dihasilkan iaitu amorfus kalsium fosfat (ACP) selepas 3 hari rendaman dengan julat nisbah Ca/P 1.2-2.02 dan pada hari ke-14 membentuk hidroskiapatit kurang kalsium (CDHA) dengan nisbah Ca/P 1.63. Perubahan fasa sampel β-wolastonit daripada keadaan hablur kepada amorfus jelas terbukti daripada keputusan XRD selepas direndam dalam SBF dengan penurunan puncak keamatan bagi sampel β-wolastonit pada sudut belauan 30°. Ini mengukuhkan mekanisme pembentukan lapisan apatit pada permukaan sampel β-wolastonit dan ianya bersifat bioaktif.
Organostannum(IV) complexes derived from dicarboxylic acids have been successfully synthesised from triphenylstannum(IV) hydroxide (Ph3SnOH). The complexes were obtained by refluxing Ph3SnOH and the respective dicarboxylic acids (malonic, succinic, glutaric and adipic acids) using a Dean and Stark apparatus in the appropriate solvent. The free ligands and complexes were characterised by C, Hand Sn elemental analysis and spectroscopic methods such as infrared and nuclear magnetic resonance (1H, 13C NMR). Structure determination of triphenylstannum(IV) succinate, 2, showed that each dicarboxylate anion was bonded monodentatically to two triphenylstannum(IV) moieties. The geometry surrounding each stannum atom is close to a tetrahedron. Spectroscopic data obtained for all complexes were found to be similar and thus it may be concluded that all complexes have the same structure as 2.
Microbial lipolytic enzymes have attracted considerable attention owing to their biotechnological potential. In this
study, thermophilic bacteria producing lipase were isolated from Bentong and Sungai Lembing hot springs, in Pahang,
Malaysia. Out of 25 colonies isolated, 14 samples showed to produce clear zones surrounding the growth on tributyrin
and trioelin agar plates. All 14 isolates showed Gram-negative bacteria with short rod morphology. PCR amplification
of 16S ribosomal DNA gene showed that these isolates were clustered with subclass β-proteobacteria consisting of
thermophilic bacteria that produce lipase. Phylogenetic analysis was carried out with the highly similar species and
4 isolates (SglA1, BtnC1, BtnC2 and BtnC3) are related to genus Caldimonas and 10 isolates (SglB1, SglB2, SglB3,
SglB4, BtnB1, BtnB2 BtnD1, BtnD2, BtnD3 and BtnD4) belonged to genus Tepidimonas. These results indicated that
novel lipase-producing thermophilic β-proteobacteria could be isolated from these hot springs.
Palm kernel cake (PKC) proteins were hydrolysed in the presence of seven proteolytic enzymes.
The antioxidant and antihypertensive activities of each protein hydrolysate were evaluated.
Papain-generated protein hydrolysate showed the highest antioxidant effects measured by
radical scavenging activity (65%), iron (Fe)(II)-chelating activity (65%), and antihypertensive
activity (71%). The results revealed a strong correlation between the antioxidant activity of
the protein hydrolysates with angiotensin-converting enzyme (ACE) inhibitory activity (R2
= 0.69 - 0.98), except for trypsin-generated hydrolysate (R2 = 0.22). The most potent protein
hydrolysate was fractionated and profiled using reversed-phase high-performance liquid
chromatography and isoelectric focusing; peptides were subsequently isolated and identified
by tandem mass spectrometry. The individual peptides were evaluated for antihypertension
potential. A positive correlation was identified between radical scavenging activity and Fe(II)-
chelating activity together and ACE inhibitory activity with R2= 0.69 - 0.98. The findings indicate that there was a positive relationship between the antioxidant and antihypertensive activities of protein hydrolysates and bioactive peptides from PKC proteins.
Postmenopausal osteoporosis is one of the main health problems in aging women. It was due to several factors including oxidative stress, which can be controlled through intake of antioxidants from food sources. Virgin coconut oil (VCO) is one of the natural product rich in antioxidants and has been proven to protect osteoporotic bone. This study was conducted to gain in-depth understanding on virgin coconut oil’s activity on osteoporosis at molecular level. Thirty two female Sprague-Dawley rats were divided into four groups, namely Sham operated group, ovariectomized control group (Ovx+Ctrl), ovariectomized with VCO treatment (Ovx+VCO), and ovariectomized with estrogen treatment (Ovx+E). All treatments were administered orally for ten weeks. Bone samples were obtained to examine changes on expression of superoxide dismutase (SOD), glutathione peroxidase (GPX), osteocalcin and runt-related transcription factor 2 (Runx2) genes. The results indicated that rats receiving VCO treatment had experienced significant increments in SOD, GPX and osteocalcin gene expressions compared to the ovariectomized control group, besides the gene expressions of Runx2 which also showed an increment pattern. In conclusion, VCO helps to protect bone in osteoporotic rat model by increasing the expressions of antioxidant genes and genes which increase the osteoblast acitivities.
Keywords: Osteoporosis; ovariectomized rat model; postmenopausal; virgin coconut oil
Urografi intravena (IVU) dan tomografi berkomputer helikal tanpa kontras (UHCT) urografi adalah dua prosedur utama
yang akan dijalankan semasa kajian radiologi bagi pengesanan urolitiasis (batu karang) pada sistem genitourinari.
Dedahan terhadap sinaran radiasi merupakan faktor kebimbangan utama dalam kedua-dua prosedur. Oleh itu, satu
kajian perbandingan dos sinaran telah dijalankan antara prosedur IVU dan UHCT urografi di samping menentukan faktor
dedahan optimum bagi kedua-dua prosedur tersebut. Kajian ini telah dijalankan ke atas fantom antropomorfi seluruh
tubuh mengikut protokol sebenar bagi prosedur UHCT urografi dan penghasilan radiografi bersiri beserta dengan
pemberian media berkontras bagi prosedur IVU. Sebanyak tiga parameter dedahan voltan tiub digunakan iaitu 75, 80
dan 85 kVp bagi prosedur IVU dan 100, 120 dan 140 kVp bagi prosedur UHCT urografi. Hasil dos sinaran bagi prosedur
IVU yang diperoleh adalah 1.40, 2.10 dan 2.79 mSv bagi 75, 80 dan 85 kVp. Manakala bagi prosedur UHCT urografi,
sebanyak 0.76, 1.32 dan 1.82 mSv dos sinaran direkodkan bagi 100, 120 dan 140 kVp. Hasil kualiti imej optimum adalah
menggunakan dedahan sebanyak 85 kVp bagi prosedur IVU dan 120 kVp bagi prosedur UHCT urografi. Kesimpulannya,
walaupun tidak terdapat perbezaan signifikan, dos sinaran yang terhasil daripada prosedur IVU adalah tekal lebih tinggi
daripada prosedur UHCT urografi.