Displaying publications 101 - 120 of 243 in total

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  1. Rohani A, Khadri MS, Lee HL
    Trop Biomed, 2006 Dec;23(2):164-71.
    PMID: 17322818 MyJurnal
    A study was carried out to investigate correlation between presence of specific microalgal species and Leptoconops biting midge larvae in its breeding habitats. Sand samples containing microalgae were collected from the beach where the midges were most commonly biting and from sand beaches which are potential as breeding habitats of Leptoconops. The survey covered sand beaches from 12 seperate islands. At all sites, the Bacillariophyta constituted the largest representatives of the microalgae community with the majority from the Naviculaceae family. A total of 24 microalgal species were identified from the sand samples collected from the study sites. Sand samples from Kentot Kecil Island had the highest number of algal species (11.0) and the highest algae species diversity ( Shanon-Weiner Diversity Index, H' = 0.884). Besar Island (Johor) had the lowest number of algal species (2.0) whereas Tengol A Island had the lowest algae species diversity (H'=0.234). Highest similarity index was recorded between sand samples collected from Tengol A Island and Tengol B Island (75.0%) followed by Besar Island (Melaka) and Tengol B Island (62.0 %). The variation between other islands were relatively high. Virtually many kinds of algae were found where Leptoconops were breeding but Fragilaria intermedia, Mastigloia minuta and Navicula advena were particularly common.
    Matched MeSH terms: Breeding
  2. Komatsu N, Lio H, Ooi HK
    Trop Biomed, 2021 Jun 01;38(2):48-52.
    PMID: 33973572 DOI: 10.47665/tb.38.2.036
    We described a new species of cockroach, Periplaneta gajajimana sp. nov., which was collected in Gajajima, Kagoshima-gun Toshimamura, Kagoshima Prefecture, Japan, on November 2012. The new species is characterized by its reddish brown to blackish brown body, smooth surface pronotum, well developed compound eyes, dark brown head apex, dark reddish brown front face and small white ocelli connected to the antennal sockets. In male, the tegmen tip reach the abdomen end or are slightly shorter, while in the female, it does not reach the abdominal end and exposes the abdomen beyond the 7th abdominal plate. We confirmed the validity of this new species by breeding the specimens in our laboratory to demonstrate that the features of the progeny were maintained for several generations. For comparison and easy identification of this new species, the key to species identification of the genus Periplaneta that had been reported in Japan to date are also presented.
    Matched MeSH terms: Breeding
  3. Fatimah, A.B., Lai, C.Y., Saari, N., Zaman, M.Z.
    MyJurnal
    Raw goat milk is recognized as one kind of nutritious food owed to its originality and
    medicinal values. This study aimed to evaluate the physico-chemical and microbiological
    qualities of locally produced raw goat milk prior any processing steps during storage. Milk
    samples passed organoleptic test and C.O.B. test were mostly (88.89 %) failed in alcohol test.
    AOAC Official method of oven drying method, Kjeldahl method and Soxhlet method were
    performed in physico-chemical analysis where results obtained were partially in lined with
    reported literature due to subjective factors of breeds, geographical areas and feeds. The locally
    produced raw goat milk’s compositions are high in water content and low in fat percentage.
    Initial total plate count, coliform count and proteolytic count tested were 3.44 log cfu/ml, 1.87
    log cfu/ml and 1.97 log cfu/ml, respectively. Storage time showed significant effect on the
    bacterial counts (p>0.05) of milk samples. Shelf-life of milk samples were kept up to 12 hours
    under ambient temperature (3.95 log cfu/ml) had not exceeded the standard limit. The shelflife
    of the milk samples were extended up to 16 days storage under refrigerated temperature
    of 4°C. The microbiological quality of the milk samples showed a significant bacteriological
    growth upon prolonged storage and high initial coliform count indicates possible poor hygienic
    practices at farm level.
    Matched MeSH terms: Breeding
  4. Wan Rozita, W.M., Yap, B.W., Veronica, S., Muhammad, A.K., Lim, K.H., Sumarni, M.G.
    MyJurnal
    A cross sectional survey was conducted in Kampung Datuk Kerarnat, an urbanized residential area in Kuala Lumpur from August to November ZOO5 to assess knowledge, attitude and preventive practices on dengue among its residents. A two stage sampling strategy was used to select survey household. An adult aged at least 21 years old was identified from each selected household and interviewed using a standardized questionnaire. Data was collected on demographics, dengue related KAP and house-types. Knowledge, attitude and practice were assessed using a scoring system. Each appropriate answer was given a point and the points for each section of the questionnaire were totalled. Knowledge was assessed as ‘good' or ‘moderate to poor’ based on arbitrary cut-off point. Out of 133 respondents interviewed, 78.2% were able to identify at least one clinical sign of dengue, 73% knew that dengue fever was caused by the bite of dengue infected Aedes mosquitoes, 71 % correctly identified breeding sites, 69.2% correctly identified the Aedes mosquito and 42% correctly reported biting times ofthe mosquito vector. Overall, knowledge
    was categorized as poor, attitude was good and dengue control and prevention practices was moderately poor. There was a weak but significant positive correlation between knowledge and practice (r=0.271, p=0.002) but there was no significant correlation between attitude with practice and between attitude with knowledge (p>0.05) . The results suggested that inspite of good attitude l
    on dengue fever, it did not correlate with good practices and good knowledge in dengue prevention. We conclude that there is a need to increase health promotion activities to increase knowledge which forms the basis for preventive practices as part of the strategy to control dengue. Preventive strategies are the only means of controlling the disease.
    Matched MeSH terms: Breeding
  5. Shakinah Salleh, Affrida Abu Hassan, Shuhaimi Shamsudin, Yahya Awang, Ab. Kahar Sandrang, Abdullah, Thohirah Lee
    MyJurnal
    Chrysanthemum morfolium is an important temperate cut flower and potted plant for Malaysian local market and exporter. Considering chrysanthemum as a popular vegetatively propagated ornamental plant, induce mutations for breeding purposes are more beneficial. Several of physical mutagens have been used in mutation breeding including x-rays, gamma rays and ion beams. Gamma rays and ion beams are from two different linear energy transfer (LET) which are low and high, respectively. The objective of this study was to compare the effectiveness of acute gamma and ion beam irradiation in generating flower colour mutations on nodal explants of Chrysanthemum morifblium cv. Reagan Red'. The nodal explants were irradiated with acute gamma (0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110 and 120 Gy) and ion beam (0, 0.5, 1.0, 2.0, 3.0, 5.0, 8.0, 10, 15, 20 and 30 Gy). The optimal dose for in vitro shoot regeneration using acute gamma was in the range of 10 to .15.0Gy and for ion beam was between 3.5 to 4.OGv. Relative biological effectiveness for ion beam was found 3.75 higher than the acute gamma. The regenerated plantlets were planted in the greenhouse at MARDI, Cameron Highland for morphological screening. The highest frequency of flower colour mutation for acute gamma was 77.8% whilst for ion beam were between 42.3 to 58.3%.
    Matched MeSH terms: Breeding
  6. Aida Nazlyn Nazari, Azhar Mohamad, Shuhaimi Shamsudin
    MyJurnal
    Assessing performance and genetic diversity of the wild material of oil palm is important for
    under- standing genetic structure of natural oil palm populations towards improvement of the
    crops. This in-formation is important for oil palm breeding programs, and also for continued exsitu
    conservation of the germplasm and breeding program in Malaysia. Mutation induction is one
    of the approaches in creating variants for selection in the breeding program. In this study, the
    effect of irradiated pollen towards pollen viability, bunches formation and number of
    parthenocarpic fruits were evaluated. Elaies guineensis Jacq. pollens were exposed to series of
    acute gamma radiation at dose 0, 10, 20, 40, 50, 100, 200, 300, 500, 100 and 2000 Gy . Pollen
    viability and pollen tube formation were disrupted in which unable the pollen to reach the ovule.
    At this stage, embryo was aborted towards formation of parthenocarpic fruits and rotten bunches.
    The study suggested that at low levels of irradiation i.e. < 200 Gy, generative nucleus partially
    damage and it is still maintaining capacity of fertilizing the egg cells for hybridization. It is
    important for breeders in understanding this finding towards novel variants of oil palm via
    mutation induction
    Matched MeSH terms: Breeding
  7. Rozhan, S., Jamsiah, M., Rahimah, A., Ang, K.T.
    MyJurnal
    Background: The COMBI concept is a novel approach by the WHO to control communicable diseases which are influenced by community behaviour. The Ministry of Health is currently evaluating its use against dengue in selected areas throughout Malaysia. COMBI doctrine differs from previous dengue campaigns. It acknowledges that the factors contributing to dengue proliferation may differ between areas. Factors for a given area are analysed, then a single precise behavioural goal to overcome those problems is formulated. To inculcate this behavioural change, the target community is subjected to an intensive campaign using Integrated Marketing Communication techniques adapted from the advertisingindustry, particularly involving volunteers from the community itself.
    Methodology: In Selangor the pilot project was implemented in Section 3 and Section 4 of Bandar Baru Bangi, in the district of Hulu Langat. Here, Aedes breeding was found to occur mainly in water containers of semi permanent nature (eg. ‘kolah’, aquatic plant jars, flower pot bases etc). A total of 172 volunteers were recruited to disperse the message of “Suluh – Suluh, Basuh - Basuh” whilst distributing leaflets and flashlights to 2666 homes. Residents were instructed to illuminate such water containers twice weekly and scrub any containers found to contain larvae. The program commenced on 23/5/2004 and lasted 16 weeks.
    Results : During this period, the initial Aedes Index of 5 was reduced to 0.96 while combined cases of Dengue Fever / Dengue Haemorraghic Fever in Sections 3 and 4 reported to the Hulu Langat District Health Office also dropped to 1 (unconfirmed).
    Conclusion : The COMBI approach in Hulu Langat successfully demonstrated that correct problem identification synergized with community engagement can potentially reduce Aedes proliferationand dengue morbidity.
    Matched MeSH terms: Breeding
  8. W. Wilonita, R. Nurliyana, D.D. Asma, M. Noorazizah, M.Y. Hirzun
    ASM Science Journal, 2013;7(2):105-112.
    MyJurnal
    Molecular markers have been intensively used in assisting breeding to reduce the time taken by conventional breeding as well as helping introgression of specific traits. Baseline analysis of known markers is crucial in developing a genetic database on disease and pest resistance for local rice germplasm which does not yet
    exist. In this study seven local rice varieties, including the popular MR219 and MRQ 74 and MRQ 76 (newly developed aromatic rice varieties), together with a foreign variety, Intani-2, were screened for genetic markers related to pest and disease resistance. One hundred and twenty-two type-related markers (SSR, STS, InDel and Allele-specific) for genes resistant to bacterial leaf blight, blast and brown planthopper were screened using PCR amplification and validated by sequencing. It was found that each variety had its own pattern of resistance. Using allele-specific markers namely pBPH9, pTA248 and Pisbdom were found to be the most efficient way to screen for the targeted genes. Of the seven varieties, MR219 and MR232 were found to have the highest distribution of markers for resistance genes against pest and diseases studied.
    Matched MeSH terms: Breeding
  9. Johari J.A., Jasmi Y.
    MyJurnal
    Population growth and rise in per capital income have influenced the demand for convenience foods and a meat protein-rich diet. The per capital consumption of beef which had increased from 3.89 kg in 1980 to 5.49 kg in 2006 (DVS, 2006/2007) had resulted in an increased in demand for beef at the rate of 6.2% annually. The local beef production although recorded a 2.5% increased over the last 10 years, is still unable to meat the increasing national demand for beef. In 2008, local production of beef is about 38,250 mt. could only meet about 26.7% of the national demand (DVS, 2008). The gap between supply and demand for beef is expected to widen in the next decades unless efforts is been made to accelerate local production. The lack of number of quality breeding stocks and unorganized breeding system had been the major factors that contribute to the slow growth of the local beef industry.
    Matched MeSH terms: Breeding
  10. Nazli MH, Halim RA, Abdullah AM, Hussin G, Samsudin AA
    Asian-Australas J Anim Sci, 2019 Feb;32(2):224-232.
    PMID: 29879832 DOI: 10.5713/ajas.18.0175
    OBJECTIVE: Apart from various climatic differences, corn harvest stage and varieties are two major factors that can influence the yield and quality of corn silage in the tropics. A study was conducted to determine the optimum harvest stage of four corn varieties for silage production in Malaysia.

    METHODS: Corn was harvested at four growth stages; silking, milk, dough, and dent stages from four varieties; Sweet Corn hybrid 926, Suwan, breeding test line (BTL) 1 and BTL 2. Using a split plot design, the treatments were then analysed based on the plant growth performance, yield, nutritive and feeding values followed by a financial feasibility study for potential commercialization.

    RESULTS: Significant differences and interactions were detected across the parameters suggesting varying responses among the varieties towards the harvest stages. Sweet Corn was best harvested early in the dough stage due to high dry matter (DM) yield, digestible nutrient, and energy content with low fibre portion. Suwan was recommended to be harvested at the dent stage when it gave the highest DM yield with optimum digestible nutrient and energy content with low acid detergent fibre. BTL 1 and BTL 2 varieties can either be harvested at dough or dent stages as the crude protein, crude fibre, DM yield, DM content, digestible nutrient and energy were not significantly different at either stage. Further financial analysis showed that only Sweet Corn production was not financially feasible while Suwan had the best financial appraisal values among the grain varieties.

    CONCLUSION: In conclusion, only the grain varieties tested had the potential for silage making according to their optimum harvest stage but Suwan is highly recommended for commercialization as it was the most profitable.

    Matched MeSH terms: Breeding
  11. Ghani AAA, Shahudin MS, Zamri-Saad M, Zuki AB, Wahid H, Kasim A, et al.
    Vet World, 2017 Jun;10(6):630-635.
    PMID: 28717314 DOI: 10.14202/vetworld.2017.630-635
    AIM: The study was conducted at a smallholder goat farm located in Labu, Negeri Sembilan, Malaysia. The objective of this study was to evaluate the effect of proper feeding program on growth performances of replacement breeder goats.

    MATERIALS AND METHODS: A total of 30 healthy female boer cross goats at the age of 4 months old with average initial live body weight (BW) of 20.05±0.5 kg were used for on-farm feeding trial to evaluate the growth performance as preparation for breeding purposes. The experimental goats were divided into two groups of 15 animals each labeled as control and treatment groups, which were kept under intensive farming system. Goats in control group were fed with normal routine feeding protocol practiced by the farmer, while goats in the treatment group were fed with new feed formulation. Throughout the experimental period, on-farm monitoring and data collection were carried out. Initial BW and body condition score (BCS) were recorded before the start of the experiment while final BW and BCS were gained after 7 months of the experimental period. Average daily gain (ADG) was calculated after the experiment end. Data on BW, ADG, and BCS were recorded from both groups for every 2 weeks and reported monthly. The feed intake for the control group was 2.8 kg/animal/day which practiced by the farmer and 3.2 kg/animal/day as new feed formulation for the treatment group.

    RESULTS: After 7 months of the experimental period, final BW shows an improvement in treatment group (39.1±1.53 kg) compared with control group (32.3±1.23 kg). The ADG in treatment group also gives promising result when comparing with control group. Goats in treatment group significantly attained better ADG than control group which were 126.7 g/day and 83.3 g/day, respectively. For the BCS, goats in the treatment group had shown an improvement where 86.67% (13 out of 15) of the group had BCS ≥3 (1-5 scoring scale) and only 66.67% (10 out of 15) of the control group had BCS ≥3.

    CONCLUSION: Therefore, it was concluded that implementation of proper feeding program as shown in treatment group give promising result to improve the growth performance of replacement breeder goats which can be adopted by the farmers to improve farm productivity.

    Matched MeSH terms: Breeding
  12. Salah N, Yimer N
    Vet World, 2017 Jun;10(6):605-609.
    PMID: 28717310 DOI: 10.14202/vetworld.2017.605-609
    BACKGROUND: Endometritis, which is one of the most common diseases in dairy cows postpartum, causes severe economic losses, including increased open days, calving intervals, and numbers of services to achieve conception.

    AIM: This study aimed to evaluate the ultrasound method and its agreement with the endometrium cytology method, which is used to diagnose cytological endometritis in beef cows. Moreover, we determined which method has higher sensitivity and specificity at 4 and 5 weeks postpartum.

    MATERIALS AND METHODS: The study was conducted 20-35 days postpartum. A total of 53 clinically healthy beef cows (28 Brangus and 25 Kedah-Kelantan breeds) from three beef farms were obtained. All cows were evaluated at 4 and 5 weeks postpartum, using ultrasound and cytobrush endometrial examination methods to diagnose cytological endometritis.

    RESULTS: Endometrial cytology result showed that 11.3% (6/53) and 9.4% (5/53) of the cows exhibited cytological endometritis 4 and 5 weeks postpartum, respectively. A weak-to-moderate agreement found between the diagnostic methods (k=0.29 - 0.50; p<0.01 and k=0.38 - 0.49) at 4 and 5 weeks postpartum respectively.

    CONCLUSION: The percentage of beef cows that were positive to cytological endometritis was low (polymorphonuclear cells, ≥8%) at 4 and 5 weeks postpartum. Results showed that the ultrasound method is useful and practical for diagnosing endometritis 4 and 5 weeks postpartum. This method exhibited 60% sensitivity, 93.8% specificity, and a 0.50 kappa value, especially when presence of intrauterine fluids and measurement of cervix diameter used in combination.

    Matched MeSH terms: Breeding
  13. Govender N, Senan S, Mohamed-Hussein ZA, Ratnam W
    Genom Data, 2017 Sep;13:11-14.
    PMID: 28626637 DOI: 10.1016/j.gdata.2017.05.008
    Shoot and inflorescence are central physiological and developmental tissues of plants. Flowering is one of the most important agronomic traits for improvement of crop yield. To analyze the vegetative to reproductive tissue transition in Jatropha curcas, gene expression profiles were generated from shoot and inflorescence tissues. RNA isolated from both tissues was sequenced using the Ilumina HiSeq 2500 platform. Differential gene expression analysis identified key biological processes associated with vegetative to reproductive tissue transition. The present data for J. curcas may inform the design of breeding strategies particularly with respect to reproductive tissue transition. The raw data of this study has been deposited in the NCBI's Sequence Read Archive (SRA) database with the accession number SRP090662.
    Matched MeSH terms: Breeding
  14. Liu Meng, Ding Gongtao, Roziah Bt. Kambol
    Science Letters, 2016;10(1):8-10.
    MyJurnal
    Cytogenetics studies in domestic animal are
    gaining importance because of their genetics and implication in
    breeding programmes. The present study describes the
    chromosome number and karyotypic characteristics of Ganjia
    sheep and comparison between males and females breeds. We
    adopt the method of cultivating somatic cells, and analyzed the
    chromosome karyotype of the Ganjia sheep. The result
    indicates the diploid chromosome of the Gangia sheep is 2n=54,
    in which 26 pairs of autosomes and a pair of sex chromosome
    were observed. Result indicates all autosomes are tip silk. The X
    chromosome was acrocentric and largest except the first three
    pairs that were metacentric. The Y chromosome was the
    smallest, biarmed and probably metacentric chromosome.
    Matched MeSH terms: Breeding
  15. Aktar-Uz-Zamana, Md., Tuhina-Khatun, Mst., Mohamed Hanafi Musa, Sahebi, Mahbod
    MyJurnal
    A resistant variety with high yielding potential is key for increasing crop production to
    fulfill the food requirement of the ever increasing world populations. Consequently, the aim of plant
    breeders is to develop high yielding varieties or cultivars that are resistant or tolerant to specific
    diseases or insects. For developing a resistant variety, it is enormously indispensable to incorporate or
    introgress the specific resistant genes of that particular disease into the recipient. Suppression
    subtractive hybridization (SSH) is a powerful technique for the identification of disease specific
    differentially expressed genes that are expressed in a resistant or susceptible variety. This paper
    presents a brief review on the SSH technique with examples focusing on the identification of the
    wheat disease specific differentially expressed genes and their defense mechanisms against fungal
    pathogens in global wheat cultivars. This review is helpful for wheat researchers for the updated
    information on the SSH technique for the identification of differentially expressed genes in the global
    wheat cultivars and varieties. Eventually, the identified genes could be used to develop the disease
    resistance variety through marker-assisted backcrossing programme or conventional breeding.
    Matched MeSH terms: Breeding; Inbreeding
  16. Taheri S, Abdullah TL, Noor YM, Padil HM, Sahebi M, Azizi P
    Data Brief, 2018 Aug;19:2452-2454.
    PMID: 30246104 DOI: 10.1016/j.dib.2018.07.038
    Curcuma alismatifolia, is an Asian crop from Zingiberaceae family, popularly used as ornamental plant in floriculture industry of Thailand and Cambodia. Different varieties with a wide range of colors can be found in species. Until now, few breeding programs have been done on this species and most commercially important cultivars are hybrids that are propagated vegetatively. In spite of other flowering plants, there is still lack of transcriptomic-based data on the functions of genes related to flower color in C. alismatifolia. The raw data presented in this article provides information on new original transcriptome data of two cultivars of C. alismatifolia by Illumina Hiseq. 4000 RNA-Seq technology which is the first ever report about this plant. The data is accessible via European Nucleotide Archive (ENA) under project number PRJEB18956.
    Matched MeSH terms: Breeding
  17. Goh HH, Ng CL, Loke KK
    Adv Exp Med Biol, 2018 11 2;1102:11-30.
    PMID: 30382566 DOI: 10.1007/978-3-319-98758-3_2
    Functional genomics encompasses diverse disciplines in molecular biology and bioinformatics to comprehend the blueprint, regulation, and expression of genetic elements that define the physiology of an organism. The deluge of sequencing data in the postgenomics era has demanded the involvement of computer scientists and mathematicians to create algorithms, analytical software, and databases for the storage, curation, and analysis of biological big data. In this chapter, we discuss on the concept of functional genomics in the context of systems biology and provide examples of its application in human genetic disease studies, molecular crop improvement, and metagenomics for antibiotic discovery. An overview of transcriptomics workflow and experimental considerations is also introduced. Lastly, we present an in-house case study of transcriptomics analysis of an aromatic herbal plant to understand the effect of elicitation on the biosynthesis of volatile organic compounds.
    Matched MeSH terms: Plant Breeding
  18. Wan-Norafikah O, Nazni W, Noramiza S, Shafa’ar-Ko’ohar S, Heah S, Nor-Azlina A, et al.
    Sains Malaysiana, 2012;41:1309-1313.
    Aedes aegypti and Aedes albopictus are the principle dengue vectors in Malaysia. The presence and distribution of Aedes larvae were studied in three different localities in Kelantan, Terengganu and Sabah, Malaysia in October 2008, November 2008 and June 2009. Two hundred (200) ovitraps per locality were placed randomly indoors and outdoors, depending on the environment of each locality. The highest mean number of Ae. aegypti and Ae. albopictus larvae per recovered ovitrap for both indoors and outdoors was obtained from Kg. Paya Rambai, Kelantan. The indoor populations of Ae. aegypti as well as the indoor and outdoor populations of Ae. albopictus in Kg. Paya Rambai, Kelantan were significantly higher than the other two study sites (p<0.05) by 1.03- and 4.67-folds, 2.36- and 5.84-folds and 1.98- and 4.00-folds, respectively. Both Ae. aegypti and Ae. albopictus were also found to breed within the same ovitraps placed indoors and outdoors in all study sites ranging from 15.22% to 31.82% of the total positive ovitraps. This study showed that both species could serve as the vectors of dengue in all study sites as indicated by the high populations recorded. The reliability and sensitivity of ovitraps in Aedes surveillance was also proven.
    Matched MeSH terms: Breeding
  19. Sandipan Gupta
    Sains Malaysiana, 2015;44:1217-1222.
    Sperata aor is a freshwater catfish of Bagridae family which is distributed throughout Indian subcontinent including India, Bangladesh, Pakistan, Nepal and Myanmar. It is a popular food fish due to its good taste and high nutritional value. Recently it has also made its entry in domestic ornamental fish markets of India and has been reported to have moderate export price too. Earlier, few works on different aspects of its morphology and biology have been carried out but no consolidated review is available on these aspects. Therefore, the current work was done to sum up all available information on these aspects to fill the information gap that will be beneficial to its future fishery. Considering all available information, knowledge on food and feeding habit of this fish species is satisfactory, except detail information on spatial variation of its breeding periodicity, proper information on other aspects are still lacking. No work has been conducted to examine its captive culture potential or for induced breeding. Further work should be done to explore its future fishery.
    Matched MeSH terms: Breeding
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