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  1. Fulltext Phytochemical profiles and inhibitory effects of Tiger Milk mushroom (Lignosus rhinocerus) extract on ovalbumin-induced airway inflammation in a rodent model of asthma
    Johnathan M, Gan SH, Ezumi MF, Faezahtul AH, Nurul AA
    BMC Complement Altern Med, 2016;16(1):167.
    PMID: 27255587 DOI: 10.1186/s12906-016-1141-x
    Lignosus rhinocerus (L. rhinocerus), which is known locally as Tiger Milk mushroom, is traditionally used in the treatment of asthma by indigenous communities in Malaysia. However, to date, its efficacy on asthma has not been confirmed by scientific studies and there is also sparse information available on its active constituents. In this study, the volatile constituent of L. rhinocerus hot water extract was investigated using gas chromatography mass spectrometry (GC-MS). The potential effects of L. rhinocerus extract for anti-asthmatic activity was further investigated on ovalbumin (OVA)-sensitized asthmatic Sprague Dawley rats.
    Matched MeSH terms: Rats, Sprague-Dawley
  2. Fulltext Subchronic toxicity study of standardized methanolic extract of Mitragyna speciosa Korth in Sprague-Dawley Rats
    Ilmie MU, Jaafar H, Mansor SM, Abdullah JM
    Front Neurosci, 2015;9:189.
    PMID: 26136645 DOI: 10.3389/fnins.2015.00189
    Mitragyna speciosa Korth, or better known as ketum, has long been used by traditional folk around Southeast Asia to prevent fatigue from working under hot tropical weather and as a replacement of opium, which can then cause addiction. To date, no findings have been reported of the toxic effect of ketum subchronically (28 days). Hence, the aim of this study was to investigate the toxicity of subchronic effect of standardized methanolic extract of ketum (SMEMS) in Sprague-Dawley rats. Rats were orally administered with 100, 200, and 500 mg/kg of SMEMS for 28 days. Body weights were recorded daily. They were terminated at day 28 to obtain data for hematology, biochemistry, and histopathology of the brain, liver, kidney, lung, heart, sciatic nerve, and spinal cord. The SMEMS affected body weight compared to control group. Biochemistry findings showed that liver and kidney were affected with the abnormal values in AST, creatinine, globulin, glucose, total protein, and urea. However, SMEMS produced toxic effect more to liver, kidney, and lung than other organs as observed histopathologically. The results suggested subchronic exposure of ketum is toxic to the physiology of the animals.
    Matched MeSH terms: Rats, Sprague-Dawley
  3. Fulltext Effects of tocotrienol and lovastatin combination on osteoblast and osteoclast activity in estrogen-deficient osteoporosis
    Abdul-Majeed S, Mohamed N, Soelaiman IN
    Evid Based Complement Alternat Med, 2012;2012:960742.
    PMID: 22927884 DOI: 10.1155/2012/960742
    Statins are HMGCoA reductase inhibitors and had been demonstrated to stimulate bone formation in rodents after high oral doses. Observational studies on patients treated with oral statins were varied. Delta-tocotrienol had been found to stimulate the cleavage of HMGCoA reductase and inhibit its activity. Tocotrienols were found to have both catabolic and anabolic effects on bone in different animal models of osteoporosis. The current study aimed to ascertain the effects of delta-tocotrienol and lovastatin combination on biochemical and static bone histomorphometric parameters in a postmenopausal rat model at clinically tolerable doses. 48 Sprague Dawley female rats were randomly divided into 6 groups: (1) baseline control group; (2) sham-operated control group; (3) ovariectomised control group; (4) ovariectomised and 11 mg/kg lovastatin; (5) ovariectomised and 60 mg/kg delta-tocotrienol; (6) ovariectomised and 60 mg/kg delta-tocotrienol + 11 mg/kg lovastatin. These treatments were given daily via oral gavage for 8 weeks. Delta-tocotrienol plus lovastatin treatment significantly increased bone formation and reduced bone resorption compared to the other groups. Therefore, the combined treatment may have synergistic or additive effects and have the potential to be used as an antiosteoporotic agent in patients who are at risk of both osteoporosis and hypercholesterolemia, especially in postmenopausal women.
    Matched MeSH terms: Rats, Sprague-Dawley
  4. Fulltext Behavioural and Electrophysiological Evidence of Impaired Learning and Memory in Male Sprague Dawley Rats following Subchronic Exposure to Standardised Methanolic Extract of Mitragyna speciosa Korth
    Ilmie MU, Mansor SM, Abdullah JM
    Malays J Med Sci, 2015 Dec;22(Spec Issue):45-51.
    PMID: 27006637 MyJurnal
    BACKGROUND: Mitragyna speciosa (MS) or ketum is primarily found in Southeast Asia, particularly in northern Malaysia and Thailand. The medicinal value of this plant has attracted significant attention from both herbal medicine practitioners and scientists worldwide. Despite having illegal consumption status, the plant merits study. We conducted a series of experiments to test our hypothesis that ketum impairs both learning and memory in rats.
    METHODS: Ketum leaves were extracted using methanol and standardised for the amount of its pure compound, mitragynine. Rats were divided into groups for a passive avoidance task and long-term potentiation (LTP) extracellular recording. In the extracellular recording condition, rats were grouped into control, MS100 (100 mg/kg of ketum extract), MS200 (200 mg/kg of ketum extract), and MS500 (500 mg/kg of ketum extract) groups. An additional group that received morphine was included in the passive avoidance task (10 mg/kg), and there were six animals per group. Rats received daily treatments orally for 28 days for both experiments.
    RESULT: Using a passive avoidance task, our data revealed that the rats' memory significantly increased with increasing doses of MS compared to the morphine-treated group. Our findings from LTP recordings showed that LTP was fully blocked by the higher doses of MS.
    CONCLUSION: We speculate on the possibility that additional factors were involved in the passive avoidance task because it was an in vivo animal study, while the LTP experiment solely involved brain slices.
    Matched MeSH terms: Rats, Sprague-Dawley
  5. Neurodevelopmental effects of prenatal Bisphenol A exposure on the role of microRNA regulating NMDA receptor subunits in the male rat hippocampus
    Nayan NM, Kadir SHSA, Husin A, Siran R
    Physiol Behav, 2024 Jun 01;280:114546.
    PMID: 38583549 DOI: 10.1016/j.physbeh.2024.114546
    Maternal bisphenol A (BPA) exposure has been reported to cause learning and memory deficits in born offspring. However, little is known that this impairment is potentially caused by epigenetic modulation on the development of NMDA receptor subunits. This study investigates the effect of prenatal BPA exposure on the hippocampal miR-19a and miR-539, which are responsible for regulating NMDA receptor subunits as well as learning and memory functions. Pregnant Sprague Dawley rats were orally administered with 5 mg/kg/day of BPA from pregnancy day 1 (PD1) until gestation day 21 (GD21), while control mothers received no BPA. The mothers were observed daily until GD21 for either a cesarean section or spontaneous delivery. The male offspring were sacrificed when reaching GD21 (fetus), postnatal days 7, 14, 21 (PND7, 14, 21) and adolescent age 35 (AD35) where their hippocampi were dissected from the brain. The expression of targeted miR-19a, miR-539, GRIN2A, and GRIN2B were determined by qRT-PCR while the level of GluN2A and GluN2B were estimated by western blot. At AD35, the rats were assessed with neurobehavioral tests to evaluate their learning and memory function. The findings showed that prenatal BPA exposure at 5 mg/kg/day significantly reduces the expression of miR-19a, miR-539, GRIN2A, and GRIN2B genes in the male rat hippocampus at all ages. The level of GluN2A and GluN2B proteins is also significantly reduced when reaching adolescent age. Consequently, the rats showed spatial and fear memory impairments when reaching AD35. In conclusion, prenatal BPA exposure disrupts the role of miR-19a and miR-539 in regulating the NMDA receptor subunit in the hippocampus which may be one of the causes of memory and learning impairment in adolescent rats.
    Matched MeSH terms: Rats, Sprague-Dawley*
  6. Oral administration of Lactobacillus casei Shirota can ameliorate the adverse effect of an acute aflatoxin exposure in Sprague Dawley rats
    Nikbakht E, Jamaluddin R, Redzwan SM, Khalesi S
    Int J Vitam Nutr Res, 2018 Jun;88(3-4):199-208.
    PMID: 31056010 DOI: 10.1024/0300-9831/a000513
    Aflatoxin B1(AFB1) is a toxic compound commonly found in some crops with an adverse health effect on human and animals. Some beneficial microorganisms (or probiotics) such as lactic acid bacteria have shown the ability to reduce the bioavailability of aflatoxins and its intestinal absorption. However, the dose and duration of aflatoxins exposure and probiotic treatment can influence the ability of probiotics to remove aflatoxins. Therefore, this research aimed to investigate the efficacy of oral probiotic Lactobacillus casei Shirota strain (LcS) induction in an acute exposure to AFB1 in rats. Experimentally, Sprague Dawley rats were divided into three groups: AFB1 only (n = 9); AFB1 treated with LcS (n = 9); and control (no AFB1 exposure) (n = 6) groups. The blood AFB1 level of rats treated with LcS was slightly lower than the untreated AFB1 induced rats (11.12 ± 0.71 vs 10.93 ± 0.69 ng g-1). Also, LcS treatment slightly moderated the liver and kidney biomarkers in AFB1 induced rats. However, a trend for a significant difference was only observed in ALT of AFB1 induced rats treated with LcS compared to their counterparts (126.11 ± 36.90 vs 157.36 ± 15.46, p = 0.06). Rats' body weight decreased in all animals force-fed with AFB1 with no significant difference between LcS treatment compared to the counterpart. In conclusion, this experiment indicated that probiotic LsC was able to slightly ameliorate the adverse effect of an acute exposure to AFB1 in rats. However, future studies with longer probiotics treatment or higher probiotics dose is required to confirm these findings.
    Matched MeSH terms: Rats, Sprague-Dawley
  7. In vivo evaluation of anticancer efficacy of drug loaded cockle shell-derived aragonite nanoparticles
    Wenliang F, Rameli MABP, Ibrahim TAT, Noor MHM, Yusof LM, Zakaria MZAB
    J Biomed Mater Res B Appl Biomater, 2019 Aug;107(6):1898-1907.
    PMID: 30597760 DOI: 10.1002/jbm.b.34282
    Doxorubicin (DOX) is an effective and commonly used anthracycline anticancer drug for the treatment of osteosarcoma (OS). However, its antitumor effect is hampered by the nonspecific distribution and significant adverse effects. Nanoparticles based drug delivery systems are promising approaches to maximize the anticancer efficacy while decrease the side effects. In this study, biogenic aragonite nanoparticles (ANPs) were developed from cockle shells and loaded with DOX. An orthotopic rat OS model was induced by UMR-106 cells tibia cavity injection. The anticancer efficacy study included five groups: normal control group, OS model group, free DOX group (2 mg/kg), DOX-ANPs 1 group (2 mg of equivalent DOX/kg) and DOX-ANPs 2 group (1.5 mg of equivalent DOX/kg). This study demonstrates that the DOX-ANPs treatment groups can significantly reduce the tumor volume and increase the surviving ratio as compared to the OS model group. In addition, these two DOX-ANPs groups showed less toxicity to the normal organs compared to the free DOX group. Furthermore, DOX-ANPs 2 group showed the similar anticancer efficacy as DOX-ANPs 1 group, which suggested that DOX loaded onto the ANPs may allow the reduction of chemotherapy doses. These results highlight the promising application of ANPs derived from cockle shells as an effective drug delivery system for a successful chemotherapy against OS. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1898-1907, 2019.
    Matched MeSH terms: Rats, Sprague-Dawley
  8. Keberkesanan ekstrak Hylocereus polyrhizus merendahkan lipid serum dan aras MDA-TBAR hati tikus teraruh hiperkolesterolemia
    Halimah Abdullah Sani, Asmazila Baharoom, Muhammad Azam Ahmad, Isma Illyani Ismail
    Sains Malaysiana, 2009;38.
    Hiperkolesterolemia merupakan suatu keadaan yang dikaitkan dengan perubahan aras lipid serum serta peningkatan aras peroksidaan lipid. Kajian ini dilakukan untuk melihat kesan ekstrak akues isi dan kulit buah Hylocereus polyrhizus (HP) terhadap jumlah kolesterol dan trigliserid (TG) serum serta aras malonaldehid (MDA-TBAR) hati tikus teraruh hiperkolesterolemia. Tikus Sprague dawley jantan diaruh menjadi hiperkolesterolemia dengan pemberian diet rat chow bersama 15% minyak sapi selama 8 minggu dan 0.02 g kolesterol secara suap paksa dua kali seminggu. Tikus-tikus diberi perlakuan ekstrak isi dan kulit buah HP, 300 mg/kg secara suap paksa selama 10 hari. Hasil menunjukkan aras kolesterol menurun secara signifikan (p<0.05) pada kedua-dua kumpulan hiperkolesterolemia yang diberi rawatan ekstrak isi dan kulit buah HP sebanyak 43.53% dan 51.36% berbanding tikus kawalan hiperkolesterolemia. Aras TG menunjukkan penurunan secara signifikan (p<0.05) sebanyak 38.0% bagi kumpulan tikus yang diberi rawatan ekstrak isi dan 42.98% bagi rawatan dengan ekstrak kulit buah HP. Peningkatan aras MDA-TBAR hati telah direncat dengan penurunan aras MDA-TBAR sebanyak 56.85% bagi kumpulan tikus yang diberi ekstrak kulit serta sedikit penurunan iaitu 10.27% bagi tikus yang diberi ekstrak isi berbanding tikus kawalan hiperkolesterolemia. Kajian ini menunjukkan bahawa kedua-dua ekstrak akues isi dan kulit buah HP merendahkan aras lipid serum serta aras MDA-TBAR hati pada tikus teraruh hiperkolesterolemia. Walau bagaimanapun, kesan ekstrak kulit lebih jelas berbanding ekstrak isi yang mungkin disebabkan oleh kandungan betasianin yang lebih tinggi dalam kulit berbanding isi buah HP.
    Matched MeSH terms: Rats, Sprague-Dawley
  9. Fulltext The effects of virgin coconut oil on postmenopausal osteoporotic bone: analyses on osteoblastogenesis and antioxidant genes. [Tindakan minyak kelapa dara terhadap tulang osteoporosis pascamenopaus: analisis gen osteoblastogenesis dan antioksidan]
    Shidqiyyah Abdul-Hamid, Norliza Muhammad, Isa Naina Mohamed
    Sains Malaysiana, 2016;45(12):1815-1822.
    Postmenopausal osteoporosis is one of the main health problems in aging women. It was due to several factors including oxidative stress, which can be controlled through intake of antioxidants from food sources. Virgin coconut oil (VCO) is one of the natural product rich in antioxidants and has been proven to protect osteoporotic bone. This study was conducted to gain in-depth understanding on virgin coconut oil’s activity on osteoporosis at molecular level. Thirty two female Sprague-Dawley rats were divided into four groups, namely Sham operated group, ovariectomized control group (Ovx+Ctrl), ovariectomized with VCO treatment (Ovx+VCO), and ovariectomized with estrogen treatment (Ovx+E). All treatments were administered orally for ten weeks. Bone samples were obtained to examine changes on expression of superoxide dismutase (SOD), glutathione peroxidase (GPX), osteocalcin and runt-related transcription factor 2 (Runx2) genes. The results indicated that rats receiving VCO treatment had experienced significant increments in SOD, GPX and osteocalcin gene expressions compared to the ovariectomized control group, besides the gene expressions of Runx2 which also showed an increment pattern. In conclusion, VCO helps to protect bone in osteoporotic rat model by increasing the expressions of antioxidant genes and genes which increase the osteoblast acitivities.
    Keywords: Osteoporosis; ovariectomized rat model; postmenopausal; virgin coconut oil
    Matched MeSH terms: Rats, Sprague-Dawley
  10. Acute and sub-chronic toxicological evaluation of Averrhoa carambola leaves in Sprague Dawley rats
    Saghir SAM, Abdulghani MAM, Alruhaimi RS, Ahmeda AF, Al-Gabri NA, Alomaisi SAMA, et al.
    Environ Sci Pollut Res Int, 2022 Dec;29(60):90058-90069.
    PMID: 35864392 DOI: 10.1007/s11356-022-22019-7
    Averrhoa carambola is a species of tree native to tropical Southeast Asia. It possesses antioxidant and anti-hyperlipidemia effects and has traditionally been used to treat a variety of ailments. However, the presence of oxalic acid in its fruits might restrict its consumption by individuals suffering from kidney disease, and caramboxin can cause neurotoxicity. In this study, we evaluated the acute and sub-chronic toxicity of the methanolic extract of A. carambola leaves (MEAC) in male and female rats. In the acute study, female rats were given a single oral dose of 5000 mg/kg of MEAC and closely examined for distinct indications of toxic effects during the first 4 h, periodically for 48 h, and daily thereafter for 14 days. Rats of both sexes were employed in the sub-chronic investigation for the 28-day repeated dose oral toxicity study. Results of the acute study revealed the safety of MEAC up to a dose of 5000 mg/kg where the rats did not show changes or signs of toxicity. In the sub-chronic toxicity study, MEAC (250, 500, and 1000 mg/kg) administration did not affect the body weight, food, and water consumption, motor coordination, behavior, or mental alertness in the treated rats. In addition, no variations in hematological or biochemical markers were found in MEAC-treated rats. In conclusion, these findings pinpoint the safety of MEAC at doses up to 5000 mg/kg. The leaves of A. carambola could be safely consumed by people with kidney disease to treat other ailments.
    Matched MeSH terms: Rats, Sprague-Dawley
  11. Targeted treatment for biofilm-based infections using PEGylated tobramycin
    Ding L, Wang G, Wang J, Peng Y, Cai S, Khan SU, et al.
    J Control Release, 2024 Aug;372:43-58.
    PMID: 38866243 DOI: 10.1016/j.jconrel.2024.06.022
    Chronic infections often involve biofilm-based bacteria, in which the biofilm results in significant resistance against antimicrobial agents and prevents eradication of the infection. The physicochemical barrier presented by the biofilm matrix is a major impediment to the delivery of many antibiotics. Previously, PEGylation has been shown to improve antibiotic penetration into biofilms in vitro. In these studies, PEGylating tobramycin was investigated both in vitro and in vivo. Two distinct PEGylated tobramycin molecules were synthesized (mPEG-SA-Tob and mPEG-AA-Tob). Then, in a P. aeruginosa biofilm in vitro model, we found that mPEG-SA-Tob can operate as a prodrug and showed 7 times more effectiveness than tobramycin (MIC80: 14 μM vs.100 μM). This improved biofilm eradication is attributable to the fact that mPEG-SA-Tob can aid tobramycin to penetrate through the biofilm and overcome the alginate-mediated antibiotic resistance. Finally, we used an in vivo biofilm-based chronic pulmonary infection rat model to confirm the therapeutic impact of mPEG-SA-Tob on biofilm-based chronic lung infection. mPEG-SA-Tob has a better therapeutic impact than tobramycin in that it cannot only stop P. aeruginosa from multiplying in the lungs but can also reduce inflammation caused by infections and prevent a recurrence infection. Overall, our findings show that PEGylated tobramycin is an effective treatment for biofilm-based chronic lung infections.
    Matched MeSH terms: Rats, Sprague-Dawley
  12. Vasorelaxant effect of 5,7,4'- Trihydroxyflavanone (Naringenin) via endothelium dependent, potassium and calcium channels in Sprague Dawley rats: Aortic ring model
    Tan CS, Tew WY, Jingying C, Yam MF
    Chem Biol Interact, 2021 Oct 01;348:109620.
    PMID: 34411564 DOI: 10.1016/j.cbi.2021.109620
    Naringenin is a naturally occurring flavanone (flavonoid) known to have bioactive effects on human health. It has been reported to show cardiovascular effects. This study aimed to investigate the possible vasorelaxant effect of naringenin and the mechanism behind it by using a Sprague Dawley rat aortic ring assay model. Naringenin caused significant vasorelaxation of endothelium-intact aortic rings precontracted with phenylephrine (pD2 = 4.27 ± 0.05; Rmax = 121.70 ± 4.04%) or potassium chloride (pD2 = 4.00 ± 0.04; Rmax = 103.40 ± 3.82%). The vasorelaxant effect decreased in the absence of an endothelium (pD2 = 3.34 ± 0.10; Rmax = 62.29 ± 2.73%). The mechanisms of the vasorelaxant effect of naringenin in the presence of antagonists were also investigated. Indomethacin, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, atropine, 4-aminopyridine, Nω-nitro-l-arginine methyl ester, glibenclamide and propranolol significantly reduced the relaxation stimulated by naringenin in the presence of endothelium. Besides that, the effect of naringenin on the voltage-operated calcium channel (VOCC) in the endothelium-intact aortic ring was studied, as was intracellular Ca2+ release from the sarcoplasmic reticulum (SR) in the endothelium-denuded aortic ring. The results showed that naringenin also significantly blocked the entry of Ca2+ via the VOCC, SERCA/SOCC and suppressed the release of Ca2+ from the SR. Thus, the vasorelaxant effect shown by naringenin mostly involve the COX pathway, the endothelium-dependent pathway via NO/sGC/prostaglandin, calcium and potassium channels.
    Matched MeSH terms: Rats, Sprague-Dawley*
  13. Fulltext Evidence to support cultivated fruiting body of Ophiocordyceps sinensis (Ascomycota)'s role in relaxing airway smooth muscle
    Booi HN, Pang LY, Lee MK, Fung SY, Ng CL, Ng ST, et al.
    J Ethnopharmacol, 2025 Jan 10;336:118727.
    PMID: 39182700 DOI: 10.1016/j.jep.2024.118727
    ETHNOPHARMACOLOGICAL RELEVANCE: Ophiocordyceps sinensis (O. sinensis) is a genus of Ascomycete fungus that is endemic to the alpine meadows of the Tibetan Plateau and adjoining Himalayas. It has been used traditionally as a tonic to improve respiratory health in ancient China as well as to promote vitality and longevity. Bioactive components found in O. sinensis such as adenosine, cordycepin, 3-deoxyadenosine, L-arginine and polysaccharides have gained increasing interest in recent years due to their antioxidative and other properties, which include anti-asthmatic, antiviral, immunomodulation and improvement of general health.

    AIM OF THE STUDY: This study's primary aim was to investigate the effect of a cultivated fruiting body of O. sinensis strain (OCS02®) on airways patency and the secondary focus was to investigate its effect on the lifespan of Caenorhabditis elegans.

    MATERIALS AND METHODS: A cultivated strain, OCS02®, was employed and the metabolic profile of its cold-water extract (CWE) was analysed through liquid chromatography-mass spectrometry (LC-MS). Organ bath approach was used to investigate the pharmacological properties of OCS02® CWE when applied on airway tissues obtained from adult male Sprague-Dawley rats. The airway relaxation mechanisms of OCS02® CWE were explored using pharmacological tools, where the key regulators in airway relaxation and constriction were investigated. For the longevity study, age-synchronised, pos-1 RNAi-treated wild-type type Caenorhabditis elegans at the L4 stage were utilised for a lifespan assay.

    RESULTS: Various glycopeptides and amino acids, particularly a high concentration of L-arginine, were identified from the LC-MS analysis. In airway tissues, OCS02® CWE induced a significantly greater concentration-dependent relaxation when compared to salbutamol. The relaxation response was significantly attenuated in the presence of NG-Nitro-L-arginine methyl ester (L-NAME), 1H-[1,2,4]oxadiazolo [4,3-a]quinoxalin-1-one (ODQ) and several K+ channel blockers. The longevity effect induced by OCS02® CWE (5 mg/mL and above) was observed in C. elegans by at least 17%.

    CONCLUSIONS: These findings suggest that the airway relaxation mechanisms of OCS02® CWE involved cGMP-dependent and cGMP-independent nitric oxide signalling pathways. This study provides evidence that the cultivated strain of OCS02® exhibits airway relaxation effects which supports the traditional use of its wild O. sinensis in strengthening respiratory health.

    Matched MeSH terms: Rats, Sprague-Dawley*
  14. Fulltext Chronic unpredictable stress (CUS) reduced phoenixin expression, induced abnormal sperm and testis morphology in male rats
    Mohamed ZI, Sivalingam M, Radhakrishnan AK, Jaafar F, Zainal Abidin SA
    Neuropeptides, 2024 Oct;107:102447.
    PMID: 38870753 DOI: 10.1016/j.npep.2024.102447
    Chronic stress caused by prolonged emotional pressure can lead to various physiological issues, including reproductive dysfunction. Although reproductive problems can also induce chronic stress, the impact of chronic stress-induced reproductive dysfunction remains contentious. This study investigates the effects of chronic unpredictable stress (CUS) on reproductive neuropeptides, sperm quality, and testicular morphology. Sixteen twelve-week-old Sprague Dawley rats were divided into two groups: a non-stress control group and a CUS-induced group. The CUS regimen involved various stressors over 28 days, with both groups undergoing behavioural assessments through sucrose-preference and forced-swim tests. Hypothalamic gene expression levels of CRH, PNX, GPR173, kisspeptin, GnRH, GnIH, and spexin neuropeptides were measured via qPCR, while plasma cortisol, luteinizing hormone (LH), and testosterone concentrations were quantified using ELISA. Seminal fluid and testis samples were collected for sperm analysis and histopathological evaluation, respectively. Results showed altered behaviours in CUS-induced rats, reflecting stress impacts. Hypothalamic corticotropin-releasing hormone (CRH) expression and plasma cortisol levels were significantly higher in CUS-induced rats compared to controls (p 
    Matched MeSH terms: Rats, Sprague-Dawley*
  15. Aqueous extract of cornsilk confers mild diuretic activity in normal rats
    W.I. Wan Rosli, M.A. Solihah, A.R. Nurhanan, W.A. Wan Amir Nizam
    Sains Malaysiana, 2015;44:1167-1174.
    Cornsilk is traditionally used to treat illnesses related to kidney and as diuretic agent. The study was performed to evaluate the effectiveness of Malaysian cornsilk in elevating diuresis and their dose response relationship in normal Sprague-Dawley rat. The diuresis activity was determined by administered the rats with different dose treatments of 400, 500, 600, 700 and 800 mg/kg. Cumulative urine was significantly increased with the dosage levels (400-600 mg/ kg) ranging from 14.06 - 20.13 mL. Cumulative urine of aqueous extract of cornsilk (AEC) at 400 mg/kg (14.06 mL) and 500 mg/kg (15.21 mL) treatments found to be significantly lower than positive control (21.25 mL). In addition, Na+ content was significantly higher compared with negative control at dosages of 500, 600, 700 and 800 mg/kg. At any rate, K+ and Cl- content of all AEC treatments were not significantly different during 24 h monitoring. The pH values were increased paralleled with the increment of AEC dosages, though it was not significant. On the other result, the ED50 of AEC was observed at 454.10 mg/kg. Malaysian AEC had shown a mild diuretic activity in elevating urine and Na+ content at dosages from 500 to 800 mg/kg. Whilst, AEC also showed an effect of potassium sparing diuretics. Thus, it is suggested that Malaysian cornsilk can be used as an alternative natural diuretic agent.
    Matched MeSH terms: Rats, Sprague-Dawley
  16. Impaired Ventrolateral Periaqueductal Gray-Ventral Tegmental area Pathway Contributes to Chronic Pain-Induced Depression-Like Behavior in Mice
    Lee MT, Peng WH, Wu CC, Kan HW, Wang DW, Teng YN, et al.
    Mol Neurobiol, 2023 Oct;60(10):5708-5724.
    PMID: 37338803 DOI: 10.1007/s12035-023-03439-z
    Chronic pain conditions within clinical populations are correlated with a high incidence of depression, and researchers have reported their high rate of comorbidity. Clinically, chronic pain worsens the prevalence of depression, and depression increases the risk of chronic pain. Individuals suffering from chronic pain and depression respond poorly to available medications, and the mechanisms underlying the comorbidity of chronic pain and depression remain unknown. We used spinal nerve ligation (SNL) in a mouse model to induce comorbid pain and depression. We combined behavioral tests, electrophysiological recordings, pharmacological manipulation, and chemogenetic approaches to investigate the neurocircuitry mechanisms of comorbid pain and depression. SNL elicited tactile hypersensitivity and depression-like behavior, accompanied by increased and decreased glutamatergic transmission in dorsal horn neurons and midbrain ventrolateral periaqueductal gray (vlPAG) neurons, respectively. Intrathecal injection of lidocaine, a sodium channel blocker, and gabapentin ameliorated SNL-induced tactile hypersensitivity and neuroplastic changes in the dorsal horn but not depression-like behavior and neuroplastic alterations in the vlPAG. Pharmacological lesion of vlPAG glutamatergic neurons induced tactile hypersensitivity and depression-like behavior. Chemogenetic activation of the vlPAG-rostral ventromedial medulla (RVM) pathway ameliorated SNL-induced tactile hypersensitivity but not SNL-elicited depression-like behavior. However, chemogenetic activation of the vlPAG-ventral tegmental area (VTA) pathway alleviated SNL-produced depression-like behavior but not SNL-induced tactile hypersensitivity. Our study demonstrated that the underlying mechanisms of comorbidity in which the vlPAG acts as a gating hub for transferring pain to depression. Tactile hypersensitivity could be attributed to dysfunction of the vlPAG-RVM pathway, while impairment of the vlPAG-VTA pathway contributed to depression-like behavior.
    Matched MeSH terms: Rats, Sprague-Dawley
  17. Fulltext The Efficacy of Gelam Honey Dressing towards Excisional Wound Healing
    Tan MK, Hasan Adli DS, Tumiran MA, Abdulla MA, Yusoff KM
    Evid Based Complement Alternat Med, 2012;2012:805932.
    PMID: 22536292 DOI: 10.1155/2012/805932
    Honey is one of the oldest substances used in wound management. Efficacy of Gelam honey in wound healing was evaluated in this paper. Sprague-Dawley rats were randomly divided into four groups of 24 rats each (untreated group, saline group, Intrasite Gel group, and Gelam honey group) with 2 cm by 2 cm full thickness, excisional wound created on neck area. Wounds were dressed topically according to groups. Rats were sacrificed on days 1, 5, 10, and 15 of treatments. Wounds were then processed for macroscopic and histological observations. Gelam-honey-dressed wounds healed earlier (day 13) than untreated and saline treated groups, as did wounds treated with Intrasite Gel. Honey-treated wounds exhibited less scab and only thin scar formations. Histological features demonstrated positive effects of Gelam honey on the wounds. This paper showed that Gelam honey dressing on excisional wound accelerated the process of wound healing.
    Matched MeSH terms: Rats, Sprague-Dawley
  18. Trends of reactive hyperaemia responses to repetitive loading on skin tissue of rats - Implications for pressure ulcer prevention
    Yapp JH, Kamil R, Rozi M, Mohtarrudin N, Loqman MY, Ezamin AR, et al.
    J Tissue Viability, 2017 Aug;26(3):196-201.
    PMID: 28438463 DOI: 10.1016/j.jtv.2017.03.002
    Tissue recovery is important in preventing tissue deterioration, which is induced by pressure and may lead to pressure ulcers (PU). Reactive hyperaemia (RH) is an indicator used to identify people at risk of PU. In this study, the effect of different recovery times on RH trend is investigated during repetitive loading. Twenty-one male Sprague-Dawley rats (seven per group), with body weight of 385-485 g, were categorised into three groups and subjected to different recovery times with three repetitive loading cycles. The first, second, and third groups were subjected to short (3 min), moderate (10 min), and prolonged (40 min) recovery, respectively, while fixed loading time and pressure (10 min and 50 mmHg, respectively). Peak hyperaemia was measured in the three cycles to determine trends associated with different recovery times. Three RH trends (increasing, decreasing, and inconsistent) were observed. As the recovery time is increased (3 min vs. 10 min vs. 40 min), the number of samples with increasing RH trend decreases (57% vs. 29% vs. 14%) and the number of samples with inconsistent RH trend increases (29% vs. 57% vs. 72%). All groups consists of one sample with decreasing RH trend (14%). Results confirm that different recovery times affect the RH trend during repetitive loading. The RH trend may be used to determine the sufficient recovery time of an individual to avoid PU development.
    Matched MeSH terms: Rats, Sprague-Dawley/blood; Rats, Sprague-Dawley/injuries
  19. Metabolism of goniothalamin in animal and microbial system
    El-Sharkawy S, Yusuf Z, Pihie AH, Ali AM
    Boll Chim Farm, 1996 Jan;135(1):35-40.
    PMID: 9004738
    Of the twenty microorganisms screened for metabolism of goniothalamin only Streptomyces aurofaciens ATCC 10762 and Nocardia species NRRL 5646 produced two metabolites, 3,4-dihydrogoniothalamin and 3,4,7,8 tetrahydrogoniothalamin. The identity of the isolated metabolites were established using TLC, HPLC, MS, IR, and 1H- and 13C-NMR spectroscopy. In addition, the substrate had been transformed into two unknown metabolites by Aspergillus niger ATCC 11394 and Septomyxa affinis ATCC 6737 in low yield. Three of the metabolites were also detected and identified in the urine and blood samples of the goniothalamin-treated Sprague-Dawley rats. The obtained results are in agreement with and support the principle of microbial models of mammalian metabolism.
    Matched MeSH terms: Rats, Sprague-Dawley
  20. Fulltext Cell viability in three ex vivo rat models of spinal cord injury
    Patar A, Dockery P, Howard L, McMahon SS
    J Anat, 2019 02;234(2):244-251.
    PMID: 30417349 DOI: 10.1111/joa.12909
    Spinal cord injury (SCI) is a devastating disorder that has a poor prognosis of recovery. Animal models of SCI are useful to understand the pathophysiology of SCI and the potential use of therapeutic strategies for human SCI. Ex vivo models of central nervous system (CNS) trauma, particularly mechanical trauma, have become important tools to complement in vivo models of injury in order to reproduce the sequelae of human CNS injury. Ex vivo organotypic slice cultures (OSCs) provide a reliable model platform for the study of cell dynamics and therapeutic intervention following SCI. In addition, these ex vivo models support the 3R concept of animal use in SCI research - replacement, reduction and refinement. Ex vivo models cannot be used to monitor functional recovery, nor do they have the intact blood supply of the in vivo model systems. However, the ex vivo models appear to reproduce many of the post traumatic events including acute and secondary injury mechanisms. Several well-established OSC models have been developed over the past few years for experimental spinal injuries ex vivo in order to understand the biological response to injury. In this study, we investigated cell viability in three ex vivo OSC models of SCI: stab injury, transection injury and contusion injury. Injury was inflicted in postnatal day 4 rat spinal cord slices. Stab injury was performed using a needle on transverse slices of spinal cord. Transection injury was performed on longitudinal slices of spinal cord using a double blade technique. Contusion injury was performed on longitudinal slices of spinal cord using an Infinite Horizon impactor device. At days 3 and 10 post-injury, viability was measured using dual staining for propidium iodide and fluorescein diacetate. In all ex vivo SCI models, the slices showed more live cells than dead cells over 10 days in culture, with higher cell viability in control slices compared with injured slices. Although no change in cell viability was observed between time-points in stab- and contusion-injured OSCs, a reduction in cell viability was observed over time in transection-injured OSCs. Taken together, ex vivo SCI models are a useful and reliable research tool that reduces the cost and time involved in carrying out animal studies. The use of OSC models provides a simple way to study the cellular consequences following SCI, and they can also be used to investigate potential therapeutics regimes for the treatment of SCI.
    Matched MeSH terms: Rats, Sprague-Dawley
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