Browse publications by year: 2017

  1. Cheng A, Mayes S, Dalle G, Demissew S, Massawe F
    Biol Rev Camb Philos Soc, 2017 Feb;92(1):188-198.
    PMID: 26456883 DOI: 10.1111/brv.12225
    There are more than 50000 known edible plants in the world, yet two-thirds of global plant-derived food is provided by only three major cereals - maize (Zea mays), wheat (Triticum aestivum) and rice (Oryza sativa). The dominance of this triad, now considered truly global food commodities, has led to a decline in the number of crop species contributing to global food supplies. Our dependence on only a few crop species limits our capability to deal with challenges posed by the adverse effects of climate change and the consequences of dietary imbalance. Emerging evidence suggests that climate change will cause shifts in crop production and yield loss due to more unpredictable and hostile weather patterns. One solution to this problem is through the wider use of underutilised (also called orphan or minor) crops to diversify agricultural systems and food sources. In addition to being highly nutritious, underutilised crops are resilient in natural and agricultural conditions, making them a suitable surrogate to the major crops. One such crop is teff [Eragrostis tef (Zucc.) Trotter], a warm-season annual cereal with the tiniest grain in the world. Native to Ethiopia and often the sustenance for local small farmers, teff thrives in both moisture-stressed and waterlogged soil conditions, making it a dependable staple within and beyond its current centre of origin. Today, teff is deemed a healthy wheat alternative in the West and is sought-after by health aficionados and those with coeliac disease or gluten sensitivity. The blooming market for healthy food is breathing new life into this underutilised crop, which has received relatively limited attention from mainstream research perhaps due to its 'orphan crop' status. This review presents the past, present and future of an ancient grain with a potential beyond its size.
    MeSH terms: Agriculture/standards*; Agriculture/trends; Edible Grain*; Food Supply/standards*; Crops, Agricultural; Eragrostis*; Climate Change
  2. Jais MH, Md Zin RR, Muhd Hanapi NA, Md Ali SA
    Appl Immunohistochem Mol Morphol, 2017 01;25(1):44-48.
    PMID: 26469327
    Ezrin, a membrane-linking protein, has been shown to play an important role in the carcinogenesis of infiltrating breast ductal carcinoma and its strong expression has been used to predict poor prognosis in patients with breast carcinoma. In this study, we compared ezrin protein distribution pattern in benign breast disease and breast cancer molecular subtypes and evaluated their association with clinicopathologic variables. A total of 376 breast cases (142 benign and 234 malignant cases) were studied. Immunohistochemical analysis for ezrin was performed and its expression was observed in terms of its distribution, intensity, and proportion of cells reactive for ezrin. Ezrin was expressed in all benign cases and 91.7% of malignant cases. Apical staining was positively associated with benign breast disease, whereas membranous and cytoplasmic staining were more frequently observed in malignant cases, specifically of hormone receptor-positive subtypes (luminal A and luminal B). Ezrin was significantly overexpressed in luminal A, luminal B, and HER2 subtypes. Reduced ezrin expression was significantly associated with triple-negative breast cancer molecular subtype. No significant association was demonstrated between ezrin expression and Her2 gene amplification, tumor grading, or staging.
    MeSH terms: Adolescent; Adult; Aged; Breast Neoplasms/metabolism*; Child; Cytoskeletal Proteins/metabolism*; Female; Humans; Immunohistochemistry; Middle Aged; Pregnancy; Retrospective Studies; Receptor, ErbB-2/metabolism*; Young Adult
  3. Shankargouda SB, Sidhu P, Kardalkar S, Desai PM
    J Prosthodont, 2017 Feb;26(2):168-171.
    PMID: 26479878 DOI: 10.1111/jopr.12385
    Residual ridge resorption is a rapid, progressive, irreversible, and inevitable process of bone resorption. Long-standing teeth and implants have been shown to have maintained the bone around them without resorption. Thus, overdenture therapy has been proven to be beneficial in situations where few remaining teeth are present. In addition to the various advantages seen with tooth-supported telescopic overdentures, a few shortcomings can also be expected, including unseating of the overdenture, increased bulk of the prosthesis, secondary caries, etc. The precise transfer of the secondary telescopic copings to maintain the spatial relationship, without any micromovement, remains the most critical step in ensuring the success of the tooth-supported telescopic prosthesis. Thus, a simple and innovative technique of splinting the secondary copings was devised to prevent distortion and micromovement and maintain its spatial relationship.
    MeSH terms: Dental Abutments; Crowns; Dental Impression Technique; Dental Materials/chemistry; Denture Retention; Dental Stress Analysis; Denture, Overlay*; Humans; Alveolar Bone Loss/physiopathology; Dental Prosthesis Design*; Dental Prosthesis, Implant-Supported/methods*
  4. Castaño-Rodríguez N, Kaakoush NO, Lee WS, Mitchell HM
    Gut, 2017 02;66(2):235-249.
    PMID: 26508508 DOI: 10.1136/gutjnl-2015-310545
    OBJECTIVE: To conduct a comprehensive global systematic review and meta-analysis on the association between Helicobacter pylori infection and IBD. As bacterial antigen cross-reactivity has been postulated to be involved in this association, published data on enterohepatic Helicobacter spp (EHS) and Campylobacter spp and IBD was also analysed.

    DESIGN: Electronic databases were searched up to July 2015 for all case-control studies on H. pylori infection/EHS/Campylobacter spp and IBD. Pooled ORs (P-OR) and 95% CIs were obtained using the random effects model. Heterogeneity, sensitivity and stratified analyses were performed.

    RESULTS: Analyses comprising patients with Crohn's disease (CD), UC and IBD unclassified (IBDU), showed a consistent negative association between gastric H. pylori infection and IBD (P-OR: 0.43, p value <1e-10). This association appears to be stronger in patients with CD (P-OR: 0.38, p value <1e-10) and IBDU (P-OR: 0.43, p value=0.008) than UC (P-OR: 0.53, p value <1e-10). Stratification by age, ethnicity and medications showed significant results. In contrast to gastric H. pylori, non H. pylori-EHS (P-OR: 2.62, p value=0.001) and Campylobacter spp, in particular C. concisus (P-OR: 3.76, p value=0.006) and C. showae (P-OR: 2.39, p value=0.027), increase IBD risk.

    CONCLUSIONS: H. pylori infection is negatively associated with IBD regardless of ethnicity, age, H. pylori detection methods and previous use of aminosalicylates and corticosteroids. Antibiotics influenced the magnitude of this association. Closely related bacteria including EHS and Campylobacter spp increase the risk of IBD. These results infer that H. pylori might exert an immunomodulatory effect in IBD.

    MeSH terms: Anti-Bacterial Agents/therapeutic use; Campylobacter*; Campylobacter Infections/complications; Campylobacter Infections/epidemiology*; Colitis, Ulcerative/epidemiology*; Crohn Disease/epidemiology*; Humans; Risk Factors; Stomach Diseases/microbiology; Stomach Diseases/epidemiology; Helicobacter pylori*; Helicobacter Infections/complications; Helicobacter Infections/epidemiology*; Protective Factors
  5. Bewersdorf JP, Hautmann O, Kofink D, Abdul Khalil A, Zainal Abidin I, Loch A
    Eur J Emerg Med, 2017 Jun;24(3):170-175.
    PMID: 26524675 DOI: 10.1097/MEJ.0000000000000344
    OBJECTIVES: The aim of the study was to identify covariates associated with 28-day mortality in septic patients admitted to the emergency department and derive and validate a score that stratifies mortality risk utilizing parameters that are readily available.

    METHODS: Patients with an admission diagnosis of suspected or confirmed infection and fulfilling at least two criteria for severe inflammatory response syndrome were included in this study. Patients' characteristics, vital signs, and laboratory values were used to identify prognostic factors for mortality. A scoring system was derived and validated. The primary outcome was the 28-day mortality rate.

    RESULTS: A total of 440 patients were included in the study. The 28-day hospital mortality rate was 32.4 and 25.2% for the derivation (293 patients) and validation (147 patients) sets, respectively. Factors associated with a higher mortality were immune-suppressed state (odds ratio 4.7; 95% confidence interval 2.0-11.4), systolic blood pressure on arrival less than 90 mmHg (3.8; 1.7-8.3), body temperature less than 36.0°C (4.1; 1.3-12.9), oxygen saturation less than 90% (2.3; 1.1-4.8), hematocrit less than 0.38 (3.1; 1.6-5.9), blood pH less than 7.35 (2.0; 1.04-3.9), lactate level more than 2.4 mmol/l (2.27; 1.2-4.2), and pneumonia as the source of infection (2.7; 1.5-5.0). The area under the receiver operating characteristic curve was 0.81 (0.75-0.86) in the derivation and 0.81 (0.73-0.90) in the validation set. The SPEED (sepsis patient evaluation in the emergency department) score performed better (P=0.02) than the Mortality in Emergency Department Sepsis score when applied to the complete study population with an area under the curve of 0.81 (0.76-0.85) as compared with 0.74 (0.70-0.79).

    CONCLUSION: The SPEED score predicts 28-day mortality in septic patients. It is simple and its predictive value is comparable to that of other scoring systems.

    MeSH terms: Decision Support Techniques*; Emergency Service, Hospital*; Humans; Middle Aged; Prognosis; Prospective Studies; Risk Factors; Reproducibility of Results; Hospital Mortality; Systemic Inflammatory Response Syndrome/diagnosis; Systemic Inflammatory Response Syndrome/mortality; Sepsis/diagnosis*; Sepsis/mortality; Vital Signs
  6. Fatima N, Hafizur RM, Hameed A, Ahmed S, Nisar M, Kabir N
    Eur J Nutr, 2017 Mar;56(2):591-601.
    PMID: 26593435 DOI: 10.1007/s00394-015-1103-y
    PURPOSE: The present study was undertaken to explore the possible anti-diabetic mechanism(s) of Emblica officinalis (EO) and its active constituent, ellagic acid (EA), in vitro and in vivo.

    METHOD: Neonatal streptozotocin-induced non-obese type 2 diabetic rats were treated with a methanolic extract of EO (250 or 500 mg/kg) for 28 days, and blood glucose, serum insulin, and plasma antioxidant status were measured. Insulin and glucagon immunostaining and morphometry were performed in pancreatic section, and liver TBARS and GSH levels were measured. Additionally, EA was tested for glucose-stimulated insulin secretion and glucose tolerance test.

    RESULTS: Treatment with EO extract resulted in a significant decrease in the fasting blood glucose in a dose- and time-dependent manner in the diabetic rats. It significantly increased serum insulin in the diabetic rats in a dose-dependent manner. Insulin-to-glucose ratio was also increased by EO treatment. Immunostaining of pancreas showed that EO250 increased β-cell size, but EO500 increased β-cells number in diabetic rats. EO significantly increased plasma total antioxidants and liver GSH and decreased liver TBARS. EA stimulated glucose-stimulated insulin secretion from isolated islets and decreased glucose intolerance in diabetic rats.

    CONCLUSION: Ellagic acid in EO exerts anti-diabetic activity through the action on β-cells of pancreas that stimulates insulin secretion and decreases glucose intolerance.

    MeSH terms: Animals; Antioxidants; Blood Glucose/analysis; Diabetes Mellitus, Experimental/drug therapy*; Diabetes Mellitus, Type 2/drug therapy*; Ellagic Acid/administration & dosage*; Fruit/chemistry; Glucagon/analysis; Glutathione/analysis; Hypoglycemic Agents*; Insulin/analysis; Insulin/blood; Liver/drug effects; Liver/chemistry; Phytotherapy; Plant Extracts/administration & dosage; Thiobarbituric Acid Reactive Substances/analysis; Phyllanthus emblica/chemistry*; Insulin-Secreting Cells/cytology; Insulin-Secreting Cells/drug effects*; Insulin-Secreting Cells/chemistry; Rats
  7. Manaf H, Justine M, Goh HT
    Motor Control, 2017 Jan;21(1):42-57.
    PMID: 26595318 DOI: 10.1123/mc.2015-0040
    Attentional loadings deteriorate straight walking performance for individuals poststroke, but its effects on turning while walking remain to be determined. Here we compared turning kinematics under three attentional loading conditions (single, dual-motor, and dual-cognitive task) between stroke survivors and healthy controls. Nine chronic stroke survivors and 10 healthy controls performed the Timed Upand- Go test while their full-body kinematics were recorded. Onset times of yaw rotation of the head, thorax and pelvis segments and head anticipation distance were used to quantify turning coordination. Results showed that stroke survivors reoriented their body segments much earlier than the controls, but they preserved the similar segmental reorientation sequence under the single-task condition. For the healthy controls, attentional loading led to an earlier axial segment reorientation, but the reorientation sequence was preserved. In contrast, the dual-cognitive task condition led to a disrupted reorientation sequence in stroke. The results indicate that turning coordination was altered in individuals poststroke, especially under the dual-task interference.
    MeSH terms: Aged; Postural Balance/physiology*; Female; Gait; Humans; Male; Middle Aged; Motor Activity/physiology*; Stroke/physiopathology*
  8. Kyaw Oo M, Mandal UK, Chatterjee B
    Pharm Dev Technol, 2017 Feb;22(1):2-12.
    PMID: 26616399 DOI: 10.3109/10837450.2015.1116568
    High melting point polymeric carrier without plasticizer is unacceptable for solid dispersion (SD) by melting method. Combined polymer-plasticizer carrier significantly affects drug solubility and tableting property of SD.
    MeSH terms: Plasticizers; Polymers; Povidone; Solubility; Tablets
  9. Maharajan MK, Rajiah K, Sze Fang KN, Lui LY
    J Cancer Educ, 2017 03;32(1):166-174.
    PMID: 26661462 DOI: 10.1007/s13187-015-0957-2
    This study was conducted to evaluate knowledge of undergraduate pharmacy students about human papillomavirus infection and their attitude towards its prevention. A cross-sectional survey was conducted in 270 undergraduate pharmacy students using a validated questionnaire to assess knowledge about human papillomavirus infection and cervical cancer and their attitudes towards human papillomavirus vaccines. Eighty-one percent of the respondents knew that human papillomavirus is a cause of cervical cancer, and 87.8 % knew that this infection is preventable. The gender of the respondents showed the strongest correlations with human papillomavirus knowledge. There were no significant correlations between the ethnic group of the respondents and their human papillomavirus-related knowledge. Higher perceptions of risk were associated with relationship status, and respondents who were in a relationship showed greater interest in vaccinating themselves; relationship status emerged as a unique predictor. The results indicated a moderately high level of knowledge and positive attitude towards human papillomavirus vaccination with few disagreements. The results of this study will help to develop and plan appropriate education campaigns for pharmacy students that aim to reduce human papillomavirus infection and, consequently, the incidence of and mortality caused by cervical cancer in Malaysia.
    MeSH terms: Uterine Cervical Neoplasms/etiology; Uterine Cervical Neoplasms/prevention & control*; Cross-Sectional Studies; Female; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Mass Screening; Patient Acceptance of Health Care/psychology; Surveys and Questionnaires; Students, Pharmacy/psychology*; Vaccination; Papillomavirus Infections/prevention & control*; Papillomavirus Vaccines/therapeutic use; Early Detection of Cancer*
  10. Patil PG, Nimbalkar-Patil S
    J Prosthodont, 2017 Jul;26(5):481-482.
    PMID: 26683255 DOI: 10.1111/jopr.12425
    Various tools are used with a dental surveyor, including analyzing rods, carbon markers, undercut gauges, and protective sheaths for a specific function. A carbon marker is a parallel-sided carbon rod used to mark the survey line on a cast or a crown on a cast. The carbon marker (with or without protective sheath) cannot differentiate more than one survey line on the cast if needed. The wear of the carbon marker along the parallel walls after repeated use may give an incorrect survey line. We suggest a simple modification in the analyzing rod to prepare a two-colored surveying tool. An analyzing rod is a parallel-sided rod used to analyze the relative parallelism of two or more surfaces of a cast and to mark survey lines on wax patterns. With the modified analyzing rod, the survey lines can be marked with two colors, and the problem of breaking of the carbon marker also can be eliminated.
  11. Ong SG, Ding HJ
    Int J Rheum Dis, 2017 Dec;20(12):2175-2178.
    PMID: 26692320 DOI: 10.1111/1756-185X.12814
    MeSH terms: Aged; Autoantibodies/immunology*; Azathioprine/adverse effects; Biopsy; Edema/chemically induced*; Edema/diagnosis; Edema/drug therapy; Edema/immunology; Glucocorticoids/administration & dosage; Humans; Hydroxymethylglutaryl CoA Reductases/immunology*; Immunosuppressive Agents/administration & dosage; Male; Muscular Diseases/chemically induced*; Muscular Diseases/diagnosis; Muscular Diseases/drug therapy; Muscular Diseases/immunology; Necrosis; Bulbar Palsy, Progressive/chemically induced*; Bulbar Palsy, Progressive/diagnosis; Bulbar Palsy, Progressive/drug therapy; Bulbar Palsy, Progressive/immunology; Prednisolone/administration & dosage; Treatment Outcome; Muscle, Skeletal/drug effects*; Muscle, Skeletal/pathology; Muscle, Skeletal/physiopathology; Muscle Weakness; Hydroxymethylglutaryl-CoA Reductase Inhibitors/adverse effects*; Hydroxymethylglutaryl-CoA Reductase Inhibitors/immunology; Simvastatin/adverse effects*; Simvastatin/immunology; Recovery of Function
  12. Lim R, Liong ML, Lau YK, Yuen KH
    Neurourol Urodyn, 2017 02;36(2):438-442.
    PMID: 26693962 DOI: 10.1002/nau.22950
    AIMS: To enable the use of the International Consultation on Incontinence Questionnaire-Urinary Incontinence Short Form (ICIQ-UI SF) and ICIQ-Lower Urinary Tract Symptoms Quality of Life (ICIQ-LUTSqol) in Southeast Asia, we translated and subsequently evaluated their validity (content and discriminant validity), reliability (internal consistency and test-retest reliability), and responsiveness in female patients with stress urinary incontinence.

    METHODS: Permission was obtained to translate the English versions into Malay and subsequently validate them, and to validate the existing Chinese versions. The translated questionnaires were taken for pilot testing. Validation was carried out for the face/content and discriminant validity. Reliability was assessed for test-retest and internal consistency using Cronbach's alpha and intraclass correlation coefficient respectively. The responsiveness was calculated via effect size and standardized response mean.

    RESULTS: Ten patients were recruited for the pilot testing. The English and Chinese versions had "substantial" or "almost perfect" agreement as measured by weighted Kappa. 284 participants (139 patients with stress urinary incontinence and 145 healthy volunteers) were included in the subsequent phases. The ICIQ-UI SF and ICIQ-LUTSqol had good discriminant validity. The ICIQ-UI SF had moderate internal consistency although the ICIQ-LUTSqol had good internal consistency. Both questionnaires had high test-retest reliability. Responsiveness was established with a moderate to large effect size and a standardized response mean.

    CONCLUSIONS: The English, Chinese, and Malay versions each proved to be valid and reliable in our Malaysian population, thereby enabling more cross-cultural research in this region. Neurourol. Neurourol. Urodynam. 36:438-442, 2017. © 2015 Wiley Periodicals, Inc.

    MeSH terms: Adult; Aged; Female; Humans; Malaysia; Middle Aged; Pilot Projects; Quality of Life; Surveys and Questionnaires*; Translations; Urinary Incontinence/diagnosis*; Reproducibility of Results
  13. Jamian S, Norhisham A, Ghazali A, Zakaria A, Azhar B
    Insect Sci, 2017 Apr;24(2):285-294.
    PMID: 26712127 DOI: 10.1111/1744-7917.12309
    Integrated pest management (IPM) is widely practiced in commercial oil palm agriculture. This management system is intended to minimize the number of attacks by pest insects such as bagworms on crops, as well as curb economic loss with less dependency on chemical pesticides. One practice in IPM is the use of biological control agents such as predatory insects. In this study, we assessed the response of predatory natural enemies to pest outbreak and water stress, and document the habitat associations of potential pest predators. The abundances of 2 predatory insect species, namely Sycanus dichotomus and Cosmolestes picticeps (Hemiptera: Reduviidae), were compared bagworm outbreak sites and nonoutbreak sites within oil palm plantations. We also examined habitat characteristics that influence the abundances of both predatory species. We found that the abundance of C. picticeps was significantly higher in bagworm outbreak sites than in nonoutbreak sites. There were no significant differences in the abundance of S. dichotomus among outbreak and non-outbreak sites. Both species responded negatively to water stress in oil palm plantations. Concerning the relationship between predatory insect abundance and in situ habitat quality characteristics, our models explained 46.36% of variation for C. picticeps and 23.17% of variation for S. dichotomus. Both species of predatory insects thrived from the planting of multiple beneficial plants in oil palm plantations. The results suggest that C. picticeps can be used as a biological agent to control bagworm populations in oil palm plantations, but S. dichotomus has no or little potential for such ecosystem service.
    MeSH terms: Animals; Malaysia; Moths*; Pest Control, Biological/methods*; Predatory Behavior; Ecosystem; Reduviidae*; Arecaceae*
  14. Khan YH, Sarriff A, Adnan AS, Khan AH, Mallhi TH, Jummaat F
    Nephrology (Carlton), 2017 Jan;22(1):25-34.
    PMID: 26718476 DOI: 10.1111/nep.12713
    AIM: Despite increase global prevalence of End stage renal disease (ESRD) and subsequent need for renal replacement therapy (RRT), relatively little is known about disease progression and prognosis of earlier stages of CKD. Current study was conducted to examine rate of CKD progression, predictors of ESRD and death.

    METHODS: A total 621 patients with estimated glomerular filtration rate (eGFR) of 15-59ml/min/1.73m(2) (CKD stage 3 & 4) were selected and followed up for 10 years or until ESRD or death, whichever occurred first. Subjects who did not meet inclusion criteria were excluded (n=1474).

    RESULTS: Annual cumulative decline in eGFR was 3.01±0.40 ml/min/1.73m(2) . Overall disease progression was observed in 60% patients while 18% died. Among patients with CKD stage 3, 21% progressed to stage 4, 10% to stage 5ND (non-dialysis) and 31% to RRT while mortality was observed in 16% patients. On the other hand, 8% patients with CKD stage 4 progressed to stage 5ND, 31% to RRT and mortality was observed in 24% cases. Patients with CVD, higher systolic blood pressure, elevated phosphate levels, heavy proteinuria, microscopic hematuria and use of diuretics were more likely to develop ESRD. Advancing age, low eGFR, low systolic blood pressure, low hemoglobin and baseline diabetes were found to be significant predictors of mortality while being female reduced risk of mortality.

    CONCLUSION: Our data suggest that, in this CKD cohort, patients were more likely to develop ESRD than death. Prime importance should be given to mild forms of CKD to retard and even reverse CKD progression.

    MeSH terms: Female; Glomerular Filtration Rate; Humans; Kidney/physiopathology; Kidney Failure, Chronic/diagnosis; Kidney Failure, Chronic/mortality; Kidney Failure, Chronic/epidemiology*; Kidney Failure, Chronic/therapy; Longitudinal Studies; Malaysia/epidemiology; Male; Retrospective Studies; Risk Factors; Time Factors; Proportional Hazards Models; Treatment Outcome; Disease Progression; Risk Assessment; Renal Insufficiency, Chronic/diagnosis; Renal Insufficiency, Chronic/mortality; Renal Insufficiency, Chronic/epidemiology*; Renal Insufficiency, Chronic/therapy; Kaplan-Meier Estimate
  15. Lee SX, Lim HN, Ibrahim I, Jamil A, Pandikumar A, Huang NM
    Biosens Bioelectron, 2017 Mar 15;89(Pt 1):673-680.
    PMID: 26718548 DOI: 10.1016/j.bios.2015.12.030
    In this study, a disposable and simple electrochemical immunosensor was fabricated for the detection of carcinoembryonic antigen. In this method, silver nanoparticles (AgNPs) were mixed with reduced graphene oxide (rGO) to modify the surface of screen-printed carbon electrode (SPE). Initially, AgNPs-rGO modified-SPEs were fabricated by using simple electrochemical deposition method. Then the carcinoembryonic antigen (CEA) was immobilized between the primary antibody and horseradish peroxidase (HRP)-conjugated secondary antibody onto AgNPs-rGO modified-SPEs to fabricate a sandwich-type electrochemical immunosensor. The proposed method could detect the CEA with a linear range of 0.05-0.50µgmL-1 and a detection limit down to 0.035µgmL-1 as compared to its non-sandwich counterpart, which yielded a linear range of 0.05-0.40µgmL-1, with a detection limit of 0.042µgmL-1. The immunosensor showed good performance in the detection of carcinoembryonic antigen, exhibiting a simple, rapid and low-cost. The immunosensor showed a higher sensitivity than an enzymeless sensor.
    MeSH terms: Carcinoembryonic Antigen/analysis*; Electrodes; Graphite/chemistry*; Horseradish Peroxidase/chemistry; Humans; Immunoassay/economics; Immunoassay/instrumentation*; Oxidation-Reduction; Oxides/chemistry; Silver/chemistry*; Biosensing Techniques/economics; Biosensing Techniques/instrumentation*; Metal Nanoparticles/ultrastructure; Metal Nanoparticles/chemistry*; Electrochemical Techniques/economics; Electrochemical Techniques/instrumentation*; Antibodies, Immobilized/chemistry; Limit of Detection
  16. Azman A, Jamir Singh PS, Sulaiman J
    J Ment Health, 2017 Apr;26(2):98-103.
    PMID: 26732363 DOI: 10.3109/09638237.2015.1124395
    BACKGROUND: Mental illness is a disease that affects millions of people every year. It not only causes stress to the mentally ill patients, but also for the family members who provide them the care. The family caregivers, therefore need some form of coping strategies in dealing with their mentally ill family members.

    AIMS: This qualitative study aims at identifying and analysing the coping strategies adopted by the family caregivers in dealing with their mentally ill family members.

    METHOD: A total of 15 family caregivers from the state of Kedah, Malaysia participated in the face-to-face semi structured interview.

    RESULTS: The study findings identified an array of coping strategies used by the family caregivers, including religious coping, emotional coping, acceptance, becoming engaged in leisure activities, and the use of traditional healing to help them cope with their mentally ill members. Suggestions and conclusions: Study suggests that the family caregivers should engage themselves in social support groups to learn about and obtain the positive coping strategies used by other caregivers who have similar experiences in caring for the mentally ill. Study also suggests that they should get appropriate training from the mental health professionals in order to enhance the caregivers' coping skills.

  17. Adeshina AM, Hashim R
    Interdiscip Sci, 2017 Mar;9(1):140-152.
    PMID: 26754740 DOI: 10.1007/s12539-015-0140-9
    Diagnostic radiology is a core and integral part of modern medicine, paving ways for the primary care physicians in the disease diagnoses, treatments and therapy managements. Obviously, all recent standard healthcare procedures have immensely benefitted from the contemporary information technology revolutions, apparently revolutionizing those approaches to acquiring, storing and sharing of diagnostic data for efficient and timely diagnosis of diseases. Connected health network was introduced as an alternative to the ageing traditional concept in healthcare system, improving hospital-physician connectivity and clinical collaborations. Undoubtedly, the modern medicinal approach has drastically improved healthcare but at the expense of high computational cost and possible breach of diagnosis privacy. Consequently, a number of cryptographical techniques are recently being applied to clinical applications, but the challenges of not being able to successfully encrypt both the image and the textual data persist. Furthermore, processing time of encryption-decryption of medical datasets, within a considerable lower computational cost without jeopardizing the required security strength of the encryption algorithm, still remains as an outstanding issue. This study proposes a secured radiology-diagnostic data framework for connected health network using high-performance GPU-accelerated Advanced Encryption Standard. The study was evaluated with radiology image datasets consisting of brain MR and CT datasets obtained from the department of Surgery, University of North Carolina, USA, and the Swedish National Infrastructure for Computing. Sample patients' notes from the University of North Carolina, School of medicine at Chapel Hill were also used to evaluate the framework for its strength in encrypting-decrypting textual data in the form of medical report. Significantly, the framework is not only able to accurately encrypt and decrypt medical image datasets, but it also successfully encrypts and decrypts textual data in Microsoft Word document, Microsoft Excel and Portable Document Formats which are the conventional format of documenting medical records. Interestingly, the entire encryption and decryption procedures were achieved at a lower computational cost using regular hardware and software resources without compromising neither the quality of the decrypted data nor the security level of the algorithms.
    MeSH terms: Algorithms*; Software*
  18. Yong KW, Safwani WKZW, Xu F, Zhang X, Choi JR, Abas WABW, et al.
    J Tissue Eng Regen Med, 2017 08;11(8):2217-2226.
    PMID: 26756982 DOI: 10.1002/term.2120
    Cryopreservation represents an efficient way to preserve human mesenchymal stem cells (hMSCs) at early culture/passage, and allows pooling of cells to achieve sufficient cells required for off-the-shelf use in clinical applications, e.g. cell-based therapies and regenerative medicine. To fully apply cryopreserved hMSCs in a clinical setting, it is necessary to evaluate their biosafety, e.g. chromosomal abnormality and tumourigenic potential. To date, many studies have demonstrated that cryopreserved hMSCs display no chromosomal abnormalities. However, the tumourigenic potential of cryopreserved hMSCs has not yet been evaluated. In the present study, we cryopreserved human adipose-derived mesenchymal stem cells (hASCs) for 3 months, using a slow freezing method with various cryoprotective agents (CPAs), followed by assessment of the tumourigenic potential of the cryopreserved hASCs after thawing and subculture. We found that long-term cryopreserved hASCs maintained normal levels of the tumour suppressor markers p53, p21, p16 and pRb, hTERT, telomerase activity and telomere length. Further, we did not observe significant DNA damage or signs of p53 mutation in cryopreserved hASCs. Our findings suggest that long-term cryopreserved hASCs are at low risk of tumourigenesis. These findings aid in establishing the biosafety profile of cryopreserved hASCs, and thus establishing low hazardous risk perception with the use of long-term cryopreserved hASCs for future clinical applications. Copyright © 2016 John Wiley & Sons, Ltd.
    MeSH terms: Adipose Tissue/metabolism*; Adipose Tissue/pathology; Cell Transformation, Neoplastic*; DNA Damage*; Female; Humans; Neoplasm Proteins/biosynthesis*; Neoplasm Proteins/genetics; Time Factors; Cryopreservation*; Mesenchymal Stromal Cells/metabolism*; Mesenchymal Stromal Cells/pathology
  19. Al Aqqad SMH, Tangiisuran B, Hyder Ali IA, Md Kassim RMN, Wong JL, Tengku Saifudin TI
    Clin Respir J, 2017 Nov;11(6):960-967.
    PMID: 26763195 DOI: 10.1111/crj.12448
    INTRODUCTION: The elderly, with chronic obstructive pulmonary disease (COPD), are at a higher risk of hospitalisation due to acute exacerbation of COPD (AECOPD). They also often encounter multiple co-morbidities.

    OBJECTIVES: This study was aimed to explore the occurrence of anxiety, depression and to identify the factors associated with hospital readmission among older patients after AECOPD discharge.

    METHODS: A multicentre prospective study was conducted in Malaysia (from 1st September 2012 till 31st September 2013) among older patients (≥60 years) hospitalised for AECOPD. Anxiety and depression were assessed on discharge using previously validated questionnaires, Generalized Anxiety Disorder-7 (GAD-7 and Geriatric Depression Scale (GDS-15), respectively. Patients were followed up for a period of 3 months after discharge.

    RESULTS: A total of 81 patients with a median age of 72 years (IQR 66.40-78.00) were recruited. Anxiety was observed in 34.57% while 38.27% had depression. Both anxiety and depression were detected in 25.93% of the patients. A history of frequent AECOPD admissions was found to be associated with developing depressive symptoms, while anxiety scores were associated with severe dyspnoea. Severe depression was more commonly identified among patients aged 60-75 and in those with a history of tuberculosis. A high readmission rate (40.74%) during the 3-month period was noticed. History of frequent AECOPD admissions (OR = 2.87; 95% CI 1.05-7.85, P = 0.040) and ischemic heart disease (IHD) (OR = 4.04; 95% CI 1.1-14.6, P = 0.032) were identified as the factors associated with the risk of hospital readmission.

    CONCLUSIONS: Anxiety and depression were found to be relatively common among older patients with AECOPD. IHD and history of frequent COPD hospitalisation were associated with short-term readmission among the elderly.

    MeSH terms: Aged; Anxiety/complications*; Anxiety/epidemiology; Anxiety/psychology; Depression/complications*; Depression/epidemiology; Depression/psychology; Female; Hospitalization/trends; Hospitalization/statistics & numerical data*; Humans; Malaysia/epidemiology; Male; Patient Discharge; Patient Readmission/statistics & numerical data; Prospective Studies; Risk Factors; Disease Progression; Pulmonary Disease, Chronic Obstructive/epidemiology; Pulmonary Disease, Chronic Obstructive/physiopathology; Pulmonary Disease, Chronic Obstructive/psychology*; Multiple Chronic Conditions/epidemiology
  20. Kamarulzaman EE, Vanderesse R, Gazzali AM, Barberi-Heyob M, Boura C, Frochot C, et al.
    J Biomol Struct Dyn, 2017 Jan;35(1):26-45.
    PMID: 26766582 DOI: 10.1080/07391102.2015.1131196
    Vascular endothelial growth factor (VEGF) and its co-receptor neuropilin-1 (NRP-1) are important targets of many pro-angiogenic factors. In this study, nine peptides were synthesized and evaluated for their molecular interaction with NRP-1 and compared to our previous peptide ATWLPPR. Docking study showed that the investigated peptides shared the same binding region as shown by tuftsin known to bind selectively to NRP-1. Four pentapeptides (DKPPR, DKPRR, TKPPR and TKPRR) and a hexapeptide CDKPRR demonstrated good inhibitory activity against NRP-1. In contrast, peptides having arginine residue at sites other than the C-terminus exhibited low activity towards NRP-1 and this is confirmed by their inability to displace the VEGF165 binding to NRP-1. Docking study also revealed that replacement of carboxyl to amide group at the C-terminal arginine of the peptide did not affect significantly the binding interaction to NRP-1. However, the molecular affinity study showed that these peptides have marked reduction in the activity against NRP-1. Pentapeptides having C-terminal arginine showed strong interaction and good inhibitory activity with NRP thus may be a good template for anti-angiogenic targeting agent.
    MeSH terms: Amino Acid Sequence; Antineoplastic Agents/pharmacology; Antineoplastic Agents/chemistry*; Binding Sites; Humans; Ligands; Models, Molecular*; Molecular Conformation; Peptides/pharmacology; Peptides/chemistry*; Protein Binding; Drug Design*; Angiogenesis Inhibitors/pharmacology; Angiogenesis Inhibitors/chemistry*; Neuropilin-1/antagonists & inhibitors; Neuropilin-1/chemistry*; Vascular Endothelial Growth Factor A/antagonists & inhibitors; Vascular Endothelial Growth Factor A/chemistry; Molecular Dynamics Simulation; Molecular Docking Simulation
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