Browse publications by year: 2019

  1. Teo SC, Liew KJ, Shamsir MS, Chong CS, Bruce NC, Chan KG, et al.
    Int J Mol Sci, 2019 May 09;20(9).
    PMID: 31075847 DOI: 10.3390/ijms20092284
    A halo-thermophilic bacterium, Roseithermus sacchariphilus strain RA (previously known as Rhodothermaceae bacterium RA), was isolated from a hot spring in Langkawi, Malaysia. A complete genome analysis showed that the bacterium harbors 57 glycoside hydrolases (GHs), including a multi-domain xylanase (XynRA2). The full-length XynRA2 of 813 amino acids comprises a family 4_9 carbohydrate-binding module (CBM4_9), a family 10 glycoside hydrolase catalytic domain (GH10), and a C-terminal domain (CTD) for type IX secretion system (T9SS). This study aims to describe the biochemical properties of XynRA2 and the effects of CBM truncation on this xylanase. XynRA2 and its CBM-truncated variant (XynRA2ΔCBM) was expressed, purified, and characterized. The purified XynRA2 and XynRA2ΔCBM had an identical optimum temperature at 70 °C, but different optimum pHs of 8.5 and 6.0 respectively. Furthermore, XynRA2 retained 94% and 71% of activity at 4.0 M and 5.0 M NaCl respectively, whereas XynRA2ΔCBM showed a lower activity (79% and 54%). XynRA2 exhibited a turnover rate (kcat) of 24.8 s-1, but this was reduced by 40% for XynRA2ΔCBM. Both the xylanases hydrolyzed beechwood xylan predominantly into xylobiose, and oat-spelt xylan into a mixture of xylo-oligosaccharides (XOs). Collectively, this work suggested CBM4_9 of XynRA2 has a role in enzyme performance.
    MeSH terms: Amino Acid Sequence; Bacteria/enzymology*; Kinetics; Phylogeny; Recombinant Proteins/metabolism; Recombinant Proteins/chemistry; Substrate Specificity; Genetic Variation*; Xylans/metabolism; Endo-1,4-beta Xylanases/genetics; Endo-1,4-beta Xylanases/metabolism*; Endo-1,4-beta Xylanases/chemistry*; Mutant Proteins/metabolism*; Mutant Proteins/chemistry; Salt-Tolerance*; Static Electricity; Protein Domains
  2. Zhang Q, Wang PI, Ong GL, Tan SH, Tan ZW, Hii YH, et al.
    Polymers (Basel), 2019 May 09;11(5).
    PMID: 31075895 DOI: 10.3390/polym11050840
    In this work, polymers of poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-triphenylamine] with side chains containing: pyrene (C1), diphenyl (C2), naphthalene (C3), and isopropyl (C6) structures were synthesized via a Suzuki coupling reaction. The structures were verified using NMR and cyclic voltammetry measurements provide the HOMO and LUMO of the polymers. The polymer with pyrene (C1) and naphthalene (C3) produced photoluminescence in the green while the polymer with the side chain containing diphenyl (C2) and isopropyl (C6) produce dual emission peaks of blue-green photoluminescence (PL). In order to examine the electroluminescence properties of the polymers, the solutions were spin-coated onto patterned ITO anode, dried, and subsequently coated with an Al cathode layer to form pristine single layer polymer LEDs. The results are compared to a standard PFO sample. The electroluminescence spectra resemble the PL spectra for C1 and C3. The devices of C2, C3, and C6 exhibit voltage-dependent EL. An additional red emission peak was detected for C2 and C6, resulting in spectra with peaks at 435 nm, 490 nm, and 625 nm. The effects of the side chains on the spectral characteristics of the polymer are discussed.
    MeSH terms: Biphenyl Compounds; Electrodes; Naphthalenes; Polymers; Pyrenes
  3. Tan BL, Norhaizan ME
    Molecules, 2019 May 09;24(9).
    PMID: 31075966 DOI: 10.3390/molecules24091801
    Despite an increase in life expectancy that indicates positive human development, a new challenge is arising. Aging is positively associated with biological and cognitive degeneration, for instance cognitive decline, psychological impairment, and physical frailty. The elderly population is prone to oxidative stress due to the inefficiency of their endogenous antioxidant systems. As many studies showed an inverse relationship between carotenoids and age-related diseases (ARD) by reducing oxidative stress through interrupting the propagation of free radicals, carotenoid has been foreseen as a potential intervention for age-associated pathologies. Therefore, the role of carotenoids that counteract oxidative stress and promote healthy aging is worthy of further discussion. In this review, we discussed the underlying mechanisms of carotenoids involved in the prevention of ARD. Collectively, understanding the role of carotenoids in ARD would provide insights into a potential intervention that may affect the aging process, and subsequently promote healthy longevity.
    MeSH terms: Aging/physiology*; Biological Availability; Carotenoids/adverse effects; Carotenoids/metabolism; Carotenoids/therapeutic use*; Carotenoids/chemistry; Diet; Disease*; Humans; Oxidative Stress
  4. Johari MI, Besari AM, Wan Ghazali WS, Yusof Z
    BMJ Case Rep, 2019 May 09;12(5).
    PMID: 31076489 DOI: 10.1136/bcr-2018-226337
    A 47-year-old Malay man who presented with fever, poor oral intake and loss of weight for 1 month duration. Further work-up revealed evidence of disseminated Salmonella infection that was further complicated with pericardial and pleural empyema. Cultures from pericardial and pleural fluids grew Salmonella species with negative serial blood cultures. Contrast enhanced CT thorax showed pleural effusion with large pericardial effusion. The patient was treated with antibiotics and drainage of pericardial and pleural empyema was done and he was discharged well.
    MeSH terms: Anti-Bacterial Agents/therapeutic use*; Drainage; Fever/microbiology*; Humans; Male; Middle Aged; Pericardial Effusion/microbiology*; Pericardial Effusion/physiopathology; Pericardial Effusion/therapy; Pleural Effusion/microbiology*; Pleural Effusion/physiopathology; Pleural Effusion/therapy; Salmonella/pathogenicity*; Salmonella Infections/diagnosis*; Salmonella Infections/drug therapy; Salmonella Infections/physiopathology; Tomography, X-Ray Computed; Weight Loss; Treatment Outcome
  5. Mostafa AA, Elshikh MS, Al-Askar AA, Hadibarata T, Yuniarto A, Syafiuddin A
    Bioprocess Biosyst Eng, 2019 Sep;42(9):1483-1494.
    PMID: 31076865 DOI: 10.1007/s00449-019-02144-3
    Due to environmental concern, the research to date has tended to focus on how textile dye removal can be carried out in a greener manner. Therefore, this study aims to evaluate the decolorization and biotransformation pathway of Mordant Orange-1 (MO-1) by Cylindrocephalum aurelium RY06 (C. aurelium RY06). Decolorization study was conducted in a batch experiment including the investigation of the effects of physio-chemical parameters. Enzymatic activity of C. aurelium RY06 during the decolorization was also investigated. Moreover, transformation and biodegradation of MO-1 by C. aurelium RY06 were observed using the gas chromatography-mass spectrometry. Manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase, and 2,3-dioxygenase enzymes were detected during the decolorization. In general, the present work concluded that the MO-1 was successfully degraded by C. aurelium RY06 and transformed to be maleic acid and to be isophtalic acid.
    MeSH terms: Azo Compounds/metabolism*; Biotransformation; Coloring Agents/metabolism*; Fungal Proteins/metabolism; Fungi/metabolism*; Maleates/metabolism; Oxidoreductases/metabolism; Phthalic Acids/metabolism; Textiles*
  6. Ting CY, Adruce SAZ, Hassali MA, Ting H, Lim CJ, Ting RS, et al.
    Trials, 2019 05 10;20(1):267.
    PMID: 31077233 DOI: 10.1186/s13063-019-3348-x
    After publication of the original article [1], the authors have notified us that there are changes to the primary outcome of the study, instrument, subject's inclusion criteria, the funding and acknowledgements. These changes were made during the recruitment of participants and after approved by the Medical Research and Ethics Committee (MREC), National Institutes of Health Malaysia, on 16th November 2018.
    MeSH terms: Malaysia; National Institutes of Health (U.S.); Publications; Publishing; United States; Ethics Committees, Research; Biomedical Research
  7. Wong CKF, Saidi NB, Vadamalai G, Teh CY, Zulperi D
    J Appl Microbiol, 2019 Aug;127(2):544-555.
    PMID: 31077517 DOI: 10.1111/jam.14310
    AIMS: This study sought to investigate the effect of bioformulation on the biocontrol efficacy, microbial viability and storage stability of a consortium of Pseudomonas aeruginosa DRB1 and Trichoderma harzianum CBF2 against Foc Tropical Race 4 (Foc-TR4).

    MATERIALS AND RESULTS: Four bioformulations consisting of dry (pesta granules, talc powder and alginate beads) and liquid formulations were evaluated for their ability to control Foc-TR4, sustain microbial populations after application and maintain microbial stability during storage. All tested bioformulations reduced disease severity (DS) by more than 43·00% with pesta granules producing the highest reduction in DS by 66·67% and the lowest area under the disease progress curve value (468·75) in a glasshouse trial. Microbial populations of DRB1 and CBF2 were abundant in the rhizosphere, rhizoplane and within the roots of bananas after pesta granules application as compared to talc powder, alginate beads and liquid formulations 84 days after inoculation (DAI). The stability of both microbial populations after 180 days of storage at 4°C was the greatest in the pesta granule formulation.

    CONCLUSION: The pesta granule formulation was a suitable carrier of biological control agents (BCA) without compromising biocontrol efficacy, microbial population and storage stability as compared to other bioformulations used in this study.

    SIGNIFICANCE AND IMPACT OF THE STUDY: Pesta granules could be utilized to formulate BCA consortia into biofertilizers. This formulation could be further investigated for possible applications under agricultural field settings.

    MeSH terms: Fusarium*; Plant Diseases/microbiology; Plant Diseases/prevention & control*; Pseudomonas aeruginosa*; Trichoderma*; Plant Roots/microbiology; Musa/microbiology*; Microbial Viability; Rhizosphere; Microbial Consortia; Biological Control Agents*
  8. Saad M, Alip A, Lim J, Abdullah MM, Chong FLT, Chua CB, et al.
    BJU Int, 2019 09;124(3):373-382.
    PMID: 31077523 DOI: 10.1111/bju.14807
    OBJECTIVE: To examine the results of the Malaysian Advanced Prostate Cancer Consensus Conference (MyAPCCC) 2018, held for assessing the generalizability of consensus reached at the Advanced Prostate Cancer Consensus Conference (APCCC 2017) to Malaysia, a middle-income country.

    METHODS: Six key sections were chosen: (1) high-risk localized and locally advanced prostate cancer, (2) oligometastatic prostate cancer, (3) castration-naïve prostate cancer, (4) castrate resistant prostate cancer, (5) use of osteoclast-targeted therapy and (6) global access to prostate cancer drugs. There were 101 consensus questions, consisting of 91 questions from APCCC 2017 and 10 new questions from MyAPCCC 2018, selected and modified by the steering committee; of which, 23 questions were assessed in both ideal world and real-world settings. A panel of 22 experts, comprising of 11 urologists and 11 oncologists, voted on 101 predefined questions anonymously. Final voting results were compared with the APCCC 2017 outcomes.

    RESULTS: Most voting results from the MyAPCCC 2018 were consistent with the APCCC 2017 outcomes. No consensus was achieved for controversial topics with little level I evidence, such as management of oligometastatic disease. No consensus was reached on using high-cost drugs in castration-naïve or castration-resistant metastatic prostate cancer in real-world settings. All panellists recommended using generic drugs when available.

    CONCLUSIONS: The MyAPCCC 2018 voting results reflect the management of advanced prostate cancer in a middle-income country in a real-world setting. These results may serve as a guide for local clinical practices and highlight the financial challenges in modern healthcare.

    MeSH terms: Health Services Accessibility; Humans; Malaysia; Male; Prostatic Neoplasms/therapy*; Societies, Medical/organization & administration*; Practice Guidelines as Topic; Consensus
  9. Chowdhury MR, Moshikur RM, Wakabayashi R, Tahara Y, Kamiya N, Moniruzzaman M, et al.
    Int J Pharm, 2019 Jun 30;565:219-226.
    PMID: 31077761 DOI: 10.1016/j.ijpharm.2019.05.020
    In order to prevent common hypersensitivity reactions to paclitaxel injections (Taxol), we previously reported an ionic liquid-mediated paclitaxel (IL-PTX) formulation with small particle size and narrow size distribution. The preliminary work showed high PTX solubility in the IL, and the formulation demonstrated similar antitumor activity to Taxol, while inducing a smaller hypersensitivity effect in in vitro cell experiments. In this study, the stability of the IL-PTX formulation was monitored by quantitative HPLC analysis, which showed that IL-PTX was more stable at 4 °C than at room temperature. The in vivo study showed that the IL-PTX formulation could be used in a therapeutic application as a biocompatible component of a drug delivery system. To assess the in-vivo biocompatibility, IL or IL-mediated formulations were administered intravenously by maintaining physiological buffered conditions (neutral pH and isotonic salt concentration). From in vivo pharmacokinetics data, the IL-PTX formulation was found to have a similar systemic circulation time and slower elimination rate compared to cremophor EL mediated paclitaxel (CrEL-PTX). Furthermore, in vivo antitumor and hypersensitivity experiments in C57BL/6 mice revealed that IL-PTX had similar antitumor activity to CrEL-PTX, but a significantly smaller hypersensitivity effect compared with CrEL-PTX. Therefore, the IL-mediated formulation has potential to be an effective and safe drug delivery system for PTX.
    MeSH terms: Animals; Antineoplastic Agents, Phytogenic/administration & dosage*; Antineoplastic Agents, Phytogenic/pharmacokinetics; Drug Hypersensitivity; Female; Glycerol/administration & dosage; Glycerol/analogs & derivatives*; Glycerol/pharmacokinetics; Melanoma/drug therapy; Mice, Inbred C57BL; Skin Neoplasms/drug therapy; Drug Delivery Systems*; Paclitaxel/administration & dosage*; Paclitaxel/pharmacokinetics; Cell Line, Tumor; Ionic Liquids/administration & dosage*; Ionic Liquids/pharmacokinetics; Administration, Intravenous
  10. Zhang MW, Yeoh FY, Du Y, Lin KA
    Sci Total Environ, 2019 Aug 15;678:466-475.
    PMID: 31077925 DOI: 10.1016/j.scitotenv.2019.04.295
    As methyltheobromine (MTB) has been increasingly detected in wastewater, it would be necessary to develop more intensive and effective approaches to remove MTB. As Co species immobilized on carbonaceous materials appears as a promising catalyst, doping carbon with nitrogen has been also validated to significantly enhance catalytic activities for Oxone activation. Therefore, it is desired to develop a composite of immobilizing Co species on N-doped carbonaceous supports for activating Oxone to degrade MTB. Unfortunately, very few studies have demonstrated such composites for activating Oxone to degrade MTB as this type of composites are conventionally prepared via complex procedures. Alternatively, this study aims to develop such a composite conveniently by using a cobaltic coordination polymer (CP) as a precursor. Specifically Co2+ and 4,4-bipyridine (BIPY) are selected for formulating a special one-dimensional CP, which is then carbonized to convert Co to Co nanoparticles (NPs) and transform BIPY to carbon nitride (CN) matrices. Because of 1-D coordinated structure of CoBIPY, the resulting magnetic Co NPs are well-distributed and protected within CN to form a magnetic Co-embedded carbon nitride composite (MCoCN). In comparison to pristine CN and Co3O4, MCoCN exhibits much higher catalytic activities to activate Oxone for degrading MTB completely within 7 min. MCoCN also shows a much lower activation energy of 24.6 kJ/mol than other reported catalysts for activating Oxone to degrade MTB. The findings of this study validate that the 1-D coordination polymer of CoBIPY is a useful precursor to prepare MCoCN for effectively activating Oxone to degrade MTB.
    MeSH terms: Carbon; Cobalt; Magnetics; Nitriles; Nitrogen; Polymers; Sulfuric Acids; Nanoparticles; Waste Water
  11. Wang Y, Bi L, Liao Y, Lu D, Zhang H, Liao X, et al.
    Ecotoxicol Environ Saf, 2019 Sep 30;180:80-87.
    PMID: 31078019 DOI: 10.1016/j.ecoenv.2019.04.066
    Ammonia emissions is an important issue during composting because it can cause secondary pollution and a significant of nitrogen loss. Based on research adding Bacillus stearothermophilus can reduce ammonia emissions during composting because it can use sugar in organic matter fermentation to produce organic acids over 50 °C. This study conducted the batch experiments by adding different concentrations of Bacillus stearothermophilus to reduce the ammonia emissions and find out its characteristic during layer manure composting by using an aerobic composting reactor with sawdust as a bulking agent. The results show that the application of Bacillus stearothermophilus can accelerate the rate of temperature and significantly decrease pH, the warming period was 2 days in the treatment with Bacillus stearothermophilus, while it was 4 days in the treatment without Bacillus stearothermophilus. Ammonia emissions were mainly occurred in warming and high temperature period during composting. The ammonia emissions in the treatment with 8.00 g/kg initial Bacillus stearothermophilus were significantly lower than the other lower Bacillus stearothermophilus treatment and control during composting (p  0.05). MiSeq System Sequencing results find that the addition of Bacillus stearothermophilus changed the bacterial community structure under warming and high-temperature periods during composting, increased the relative abundance of lactic acid bacillus and nitrification bacteria. Therefore, the reason for the low ammonia emission in 8.00 g/kg initial Bacillus stearothermophilus treatments might be not only due to the Bacillus stearothermophilus itself, but also Bacillus stearothermophilus can change the indigenous microorganism community, including increase the relative content of lactic acid Bacillus and nitrification bacteria, thus reducing the pH and promoting nitrification, and reducing ammonia emissions.
    MeSH terms: Ammonia/analysis*; Animals; Geobacillus stearothermophilus/growth & development*; Fermentation; Manure/microbiology*; Nitrates/analysis; Nitrogen/analysis; Soil/chemistry; Temperature; Nitrification
  12. Khan A, Khan AH, Adnan AS, Sulaiman SAS, Mushtaq S
    BMC Public Health, 2019 May 09;19(1):531.
    PMID: 31072378 DOI: 10.1186/s12889-019-6796-z
    BACKGROUND: Even though depression is one of the most common psychiatric disorders, it is under-recognized in hemodialysis (HD) patients. Existing literature does not provide enough information on evaluation of predictors of depression among HD patients. The objective of the current study was to determine the prevalence and predictors of depression among HD patients.

    METHODS: A multicenter prospective follow-up study. All eligible confirmed hypertensive HD patients who were consecutively enrolled for treatment at the study sites were included in the current study. HADS questionnaire was used to assess the depression level among study participants. Patients with physical and/or cognitive limitations that prevent them from being able to answer questions were excluded.

    RESULTS: Two hundred twenty patients were judged eligible and completed questionnaire at the baseline visit. Subsequently, 216 and 213 patients completed questionnaire on second and final follow up respectively. The prevalence of depression among patients at baseline, 2nd visit and final visit was 71.3, 78.2 and 84.9% respectively. The results of regression analysis showed that treatment given to patients at non-governmental organizations (NGO's) running HD centers (OR = 0.347, p-value = 0.039) had statistically significant association with prevalence of depression at final visit.

    CONCLUSIONS: Depression was prevalent in the current study participants. Negative association observed between depression and hemodialysis therapy at NGO's running centers signifies patients' satisfaction and better depression management practices at these centers.

    MeSH terms: Adult; Aged; Depression/epidemiology; Female; Follow-Up Studies; Renal Dialysis/psychology*; Humans; Kidney Failure, Chronic/psychology*; Kidney Failure, Chronic/therapy; Male; Middle Aged; Prospective Studies; Quality of Life/psychology*; Surveys and Questionnaires; Risk Factors; Severity of Illness Index*; Prevalence; Patient Satisfaction
  13. Liew NY, Chong YY, Yeow SH, Kua KP, Saw PS, Lee SWH
    Int J Clin Pharm, 2019 Aug;41(4):895-902.
    PMID: 31073975 DOI: 10.1007/s11096-019-00843-1
    Background Polypharmacy has been associated with an increased risk of morbidity and mortality among older adults especially those living in nursing homes. Explicit criteria to evaluate the appropriateness of medications have recently been updated. Objective To estimate and compare the prevalence of potentially inappropriate medications (PIMs) among older adults in nursing homes using screening tool for older people's prescription (STOPP-2) and the 2015 Beers criteria. Setting Nursing care homes in the Klang Valley, Malaysia. Method A cross-sectional survey of medications used among residents living in nursing homes was performed. The 2015 Beers criteria and STOPP-2 criteria were used to evaluate the use of PIMs among this population. These PIMs were subsequently classified using the Pharmaceutical Network Europe classification (PCNE). Main outcome measure Number of PIMs among residents using both criteria. Results A total of 155 residents were recruited into the study. The prevalence of PIMs was 9.7% using the STOPP-2 criteria, 17.6% using the 2015 Beers criteria and 21.3% when both tools were used in combination. The PCNE criteria identified a total of 198 drug related issues, with an average of 1.27 issue per resident. Factors associated with higher risk of having PIM include multiple medication use and history of psychiatric disorder. Conclusion The 2015 Beers criteria was able to identify more PIM's compared to STOPP-2 criteria. The findings also support the expansion of pharmacists' services in nursing homes in Malaysia.
    MeSH terms: Potentially Inappropriate Medication List/statistics & numerical data*; Aged; Cross-Sectional Studies; Female; Humans; Malaysia; Male; Middle Aged; Nursing Homes/statistics & numerical data*; Risk Factors; Prevalence
  14. Teck KS, Mac Guad R, Van Rostenberghe AH, Hua GS
    J Med Virol, 2019 09;91(9):1608-1615.
    PMID: 31074499 DOI: 10.1002/jmv.25500
    Respiratory syncytial virus (RSV) is a common pathogen affecting the respiratory tract in infants. To date, there is limited data on RSV occurrence in Malaysia especially in the northeast of Peninsular Malaysia which is significantly affected by the rainy (monsoon) season. This study aimed to determine the prevalence, risk factors (the presence of a male sibling and older school-age siblings, parental education level, monthly income, chronic lung disease, immunocompromised, being a passive smoker, multipara, breastfeeding, prematurity, congenital heart disease, nursery attendance, and rainy season) as well as clinical manifestations of RSV in hospitalized infants and children with lower respiratory tract infection (LRTI). Patients' nasopharyngeal aspirates were tested for RSV antigen, questionnaires, and seasonal variations were used to assess RSV infection. Approximately 22.6% of children were infected with RSV; mean age 7.68 ± 5.45 months. The peak incidence of RSV as a causative agent for LRTI in infants was less than or equal to 1-year old (83%) with approximately 50.5% of the affected children in the younger age group (6 months amd below). RSV infection was significantly but independently associated with the rainy season (odds ratio, 3.307; 95% confidence interval, 1.443-3.688; P 
    MeSH terms: Child, Preschool; Female; Humans; Infant; Malaysia/epidemiology; Male; Population Surveillance; Prospective Studies; Respiratory Tract Infections/diagnosis; Respiratory Tract Infections/epidemiology*; Respiratory Tract Infections/virology*; Risk Factors; Seasons; Socioeconomic Factors; Prevalence; Respiratory Syncytial Virus, Human*; Respiratory Syncytial Virus Infections/diagnosis; Respiratory Syncytial Virus Infections/epidemiology*; Respiratory Syncytial Virus Infections/virology*
  15. Karobari MI, Noorani TY, Halim MS, Dummer PMH, Ahmed HMA
    Int Endod J, 2019 Jun;52(6):917-919.
    PMID: 31074504 DOI: 10.1111/iej.13106
    MeSH terms: Dental Pulp Cavity*; Molar; Root Canal Therapy; Tooth Root*
  16. Bilal M, Gani A, Lali MIU, Marjani M, Malik N
    Cyberpsychol Behav Soc Netw, 2019 Jul;22(7):433-450.
    PMID: 31074639 DOI: 10.1089/cyber.2018.0670
    Social media has taken an important place in the routine life of people. Every single second, users from all over the world are sharing interests, emotions, and other useful information that leads to the generation of huge volumes of user-generated data. Profiling users by extracting attribute information from social media data has been gaining importance with the increasing user-generated content over social media platforms. Meeting the user's satisfaction level for information collection is becoming more challenging and difficult. This is because of too much noise generated, which affects the process of information collection due to explosively increasing online data. Social profiling is an emerging approach to overcome the challenges faced in meeting user's demands by introducing the concept of personalized search while keeping in consideration user profiles generated using social network data. This study reviews and classifies research inferring users social profile attributes from social media data as individual and group profiling. The existing techniques along with utilized data sources, the limitations, and challenges are highlighted. The prominent approaches adopted include Machine Learning, Ontology, and Fuzzy logic. Social media data from Twitter and Facebook have been used by most of the studies to infer the social attributes of users. The studies show that user social attributes, including age, gender, home location, wellness, emotion, opinion, relation, influence, and so on, still need to be explored. This review gives researchers insights of the current state of literature and challenges for inferring user profile attributes using social media data.
    MeSH terms: Data Collection/methods*; Female; Humans; Male; Personal Satisfaction; Social Identification*; Social Media*
  17. Jiang B, Sun J, Lv A, Hu X, Shi H, Sung Y, et al.
    FEMS Microbiol Lett, 2019 05 01;366(9).
    PMID: 31074797 DOI: 10.1093/femsle/fnz099
    Two DNA extraction methods, the Zirmil-beating cell disruption method (ZBC) and the QIAamp fast DNA stool mini kit (QIA), were used to extract DNA from the intestinal flora of the penaeid shrimp Litopenaeus vannamei, and their microbial communities were analyzed using 16S rDNA high-throughput sequencing. Results were obtained in terms of the number of reads, alpha diversity indexes, beta diversity indexes and taxonomic composition. The alpha diversity indexes of the community, according to the ZBC method, were higher than those according to the QIA method. Furthermore, results from the three samples using the ZBC method were less consistent than those where the QIA method was used. Further, using the latter method led to substantive clustering. It is suggested that the QIA method is more stable and repeatable than the ZBC method. Although the two extraction methods shared the major abundant microflora based on 16S rDNA high-throughput sequencing, bias associated with diversity analysis indexes and certain species was observed.
    MeSH terms: Gastrointestinal Microbiome; Animals; DNA, Ribosomal; Feces; Plant Extracts; Cluster Analysis; Penaeidae; High-Throughput Nucleotide Sequencing; Microbiota
  18. Mubarak N, Hatah E, Aris MAM, Shafie AA, Zin CS
    PLoS One, 2019;14(5):e0216563.
    PMID: 31075110 DOI: 10.1371/journal.pone.0216563
    BACKGROUND: The general problem is lack of inter-professional collaboration and the way private primary care responds to manage chronic diseases in Malaysia. Absence of prescription review, inadequate patient education, the highest percentage of prescribing errors and half of the chronic disease patients are nonadherent. Medicines are the most common and life long used interventions in chronic diseases. Hence, the need to manage medicine in chronic diseases becomes obligatory. As both general practitioner and community pharmacist can dispense medications, this has resulted in a business rivalry. There is a need to build consensus among various healthcare stakeholders for a collaborative medication therapy management model (CMTM) where community pharmacist has an active role in chronic care.

    METHOD: This study utilized modified e-Delphi method to build consensus. A validated e-Delphi survey was administered to a purposive sample of 29 experts. Consensus was pre-defined to be the point where >85% of the experts fall in either agree or strongly agree category for each statement. The inter-expert agreement was computed in both rounds using Intra-class correlation coefficient and Kendall's W. Delphi operates in an iterative fashion till there comes stability in responses. At the end of each round, experts were provided aggregate response, their own response and choice to change their response in the light of aggregate response.

    RESULTS: Response rate was 70.73% and 100% in 1st and 2nd round, respectively. Consensus was achieved on 119/132 statements which mainly referred to the need, structural and regulatory aspects of CMTM model in Malaysia. However, there were some flashpoints on dispensing separation and means to finance this model. Stability in response of experts was achieved after 2nd round; hence, no next round was executed.

    CONCLUSION: Overall, the study findings witnessed the expert panel's support for the CMTM model. Study helped to sketch CMTM model and facilitated development of some recommendations to the authorities which may help to formulate a policy to bring CPs under a working relationship with GPs. Hence, this study should be taken as a call for redefining of the roles of CPs and GPs in Malaysia.

    MeSH terms: Chronic Disease/drug therapy*; Delphi Technique; Female; Humans; Malaysia; Male; Pharmacists; Practice Guidelines as Topic; Consensus*; Medication Therapy Management/organization & administration*; General Practitioners
  19. Ali MK, Moshikur RM, Wakabayashi R, Tahara Y, Moniruzzaman M, Kamiya N, et al.
    J Colloid Interface Sci, 2019 Sep 01;551:72-80.
    PMID: 31075635 DOI: 10.1016/j.jcis.2019.04.095
    Ionic liquid (IL) surfactants have attracted great interest as promising substitutes for conventional surfactants owing to their exceptional and favorable physico-chemical properties. However, most IL surfactants are not eco-friendly and form unstable micelles, even when using a high concentration of the surfactant. In this study, we prepared a series of halogen-free and biocompatible choline-fatty-acid-based ILs with different chain lengths and degrees of saturation, and we then investigated their micellar properties in aqueous solutions. Characterization of the synthesized surface-active ILs (SAILs) was performed by 1H and 13C nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, and elemental analysis. The surface-active properties of the SAILs were investigated by tensiometry, conductometry, and dynamic light scattering measurements. The critical micelle concentration of the SAILs was found to be 2-4 times lower than those of conventional surfactants. The thermodynamic properties of micellization (ΔG0m, ΔH0m, and ΔS0m) indicate that the micellization process of the SAILs is spontaneous, stable, and entropy-driven at room temperature. The cytotoxicity of the SAILs was evaluated using mammalian cell line NIH 3T3. Importantly, [Cho][Ole] shows lower toxicity than the analogous ILs with conventional surfactants. These results clearly suggest that these environmentally friendly SAILs can be used as a potential alternative to conventional ILs for various purposes, including biological applications.
    MeSH terms: Animals; Biocompatible Materials/chemistry*; Cell Survival/drug effects; Choline/chemistry*; Fatty Acids/chemistry*; Micelles; Surface Properties; Surface-Active Agents/toxicity; Surface-Active Agents/chemistry*; Temperature; Thermodynamics; Water; 3T3 Cells; Mice; Ionic Liquids/toxicity; Ionic Liquids/chemistry*
  20. Akkerman O, Aleksa A, Alffenaar JW, Al-Marzouqi NH, Arias-Guillén M, Belilovski E, et al.
    Int J Infect Dis, 2019 Jun;83:72-76.
    PMID: 30953827 DOI: 10.1016/j.ijid.2019.03.036
    The World Health Organization launched a global initiative, known as aDSM (active TB drug safety monitoring and management) to better describe the safety profile of new treatment regimens for drug-resistant tuberculosis (TB) in real-world settings. However, comprehensive surveillance is difficult to implement in several countries. The aim of the aDSM project is to demonstrate the feasibility of implementing national aDSM registers and to describe the type and the frequency of adverse events (AEs) associated with exposure to the new anti-TB drugs. Following a pilot study carried out in 2016, official involvement of TB reference centres/countries into the project was sought and cases treated with bedaquiline- and/or delamanid-containing regimens were consecutively recruited. AEs were prospectively collected ensuring potential attribution of the AE to a specific drug based on its known safety profile. A total of 309 cases were fully reported from 41 centres in 27 countries (65% males; 268 treated with bedaquiline, 20 with delamanid, and 21 with both drugs) out of an estimated 781 cases the participating countries had committed to report by the first quarter of 2019.
    MeSH terms: Antitubercular Agents/adverse effects*; Drug Therapy, Combination; Feasibility Studies; Female; Humans; Male; Nitroimidazoles/administration & dosage; Nitroimidazoles/adverse effects*; Oxazoles/administration & dosage; Oxazoles/adverse effects*; Pilot Projects; Tuberculosis/drug therapy; World Health Organization; Tuberculosis, Multidrug-Resistant/drug therapy*; Diarylquinolines/administration & dosage; Diarylquinolines/adverse effects*
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