Browse publications by year: 2021

  1. Dalal R, Shah J, Gorain B, Choudhury H, Jacob S, Mehta TA, et al.
    AAPS PharmSciTech, 2021 Oct 04;22(7):244.
    PMID: 34608546 DOI: 10.1208/s12249-021-02132-5
    Asenapine, an atypical antipsychotic agent, has been approved for the acute and maintenance treatment of schizophrenia and manic episodes of bipolar disorder. However, the extensive hepatic metabolism limits its oral bioavailability. Therefore, the objective of the current investigation was to develop sublingual film containing asenapine to enhance the therapeutic efficacy. Sublingual films containing asenapine were fabricated using polyethylene oxide and hydroxypropyl methylcellulose by solvent casting method. Design of experiment was used as a statistical tool to optimize the proportion of the film-forming polymers in order to establish the critical quality attributes of the drug formulation. The process was studied in detail by assessing risk of each step as well as parameters and material attributes to reduce the risk to a minimum. A control strategy was defined to ensure manufacture of films according to the target product profile by evaluation of intermediate quality attributes at the end of each process step. Results of optimized formulations showed rapid disintegration, adequate folding endurance, good percentage elongation, tensile strength, and viscosity. Besides, the results from the in vitro dissolution/ex vivo permeation studies showed rapid dissolution (100% in 6 min) and higher asenapine permeation (~ 80% in 90 min) through the sublingual epithelium. In vivo study indicates greater asenapine absorption (31.18 ± 5.01% of administered dose) within 5 min and was comparable with marketed formulation. In summary, the designing plan to develop asenapine formulation was successfully achieved with desired characteristics of the delivery tool for sublingual administration.
    MeSH terms: Dibenzocycloheptenes; Heterocyclic Compounds with 4 or More Rings; Humans
  2. Choudhury A, Vijayaraghavan R, Maiwall R, Kumar M, Duan Z, Yu C, et al.
    Hepatol Int, 2021 Oct 04.
    PMID: 34608586 DOI: 10.1007/s12072-021-10206-6
    BACKGROUND AND AIMS: Acute-on-chronic liver failure (ACLF) is a rapidly progressive illness with high short-term mortality. Timely liver transplant (LT) may improve survival. We evaluated various indices for assessment of the severity of liver failure and their application for eligibility and timing of living donor LT (LDLT).

    METHODS: Altogether 1021 patients were analyzed for the severity and organ failure at admission to determine transplant eligibility and 28 day survival with or without transplant.

    RESULTS: The ACLF cohort [mean age 44 ± 12.2 years, males 81%) was of sick patients; 55% willing for LT at admission, though 63% of them were ineligible due to sepsis or organ failure. On day 4, recovery in sepsis and/or organ failure led to an improvement in transplant eligibility from 37% at baseline to 63.7%. Delay in LT up to 7 days led to a higher incidence of multiorgan failure (p 

  3. Kow CS, Ramachandram DS, Hasan SS
    J Food Biochem, 2021 Nov;45(11):e13959.
    PMID: 34608650 DOI: 10.1111/jfbc.13959
    MeSH terms: Eating; Humans; Immunity, Cellular; beta-Glucans*
  4. Mehta M, Malyla V, Paudel KR, Chellappan DK, Hansbro PM, Oliver BG, et al.
    J Food Biochem, 2021 11;45(11):e13954.
    PMID: 34609010 DOI: 10.1111/jfbc.13954
    Metastasis represents the leading cause of death in lung cancer patients. C-X-C Motif Chemokine Ligand 8 (CXCL-8), Chemokine (C-C motif) ligand 20 (CCL-20) and heme oxygenase -1 (HO-1) play an important role in cancer cell proliferation and migration. Berberine is an isoquinoline alkaloid isolated from several herbs in the Papaveraceae family that exhibits anti-inflammatory, anticancer and antidiabetic properties. Therefore, the aim of present study is to investigate the inhibitory potential of berberine monoolein loaded liquid crystalline nanoparticles (berberine-LCNs) against cancer progression. Berberine-LCNs were prepared by mixing berberine, monoolein and poloxamer 407 (P407) using ultrasonication method. A549 cells were treated with or without 5 µM dose of berberine LCNs for 24 hr and total cellular protein was extracted and further analyzed for the protein expression of CCl-20, CXCL-8 and HO-1 using human oncology array kit. Our results showed that berberine-LCNs significantly reduced the expression of CCl-20, CXCL-8 and HO-1 at dose of 5µM. Collectively, our findings suggest that berberine-LCNs have inhibitory effect on inflammation/oxidative stress related cytokines i.e. CCL20, CXCL-8, and HO-1 which could be a novel therapeutic target for the management of lung cancer. PRACTICAL APPLICATIONS: Berberine is an isoquinoline alkaloid extracted from various plants of Papaveraceae family. CXCL-8, CCL-20 and HO-1 play an important role in cancer progression. Our study showed that Berberine LCNs significantly downregulate the expression of CXCL-8, CCL-20 and HO-1 which suggests that Berberine loaded nanoparticles could be a promising therapeutic alternative for the management of lung cancer.
  5. Wong YH, Wong SH, Wong XT, Yi Yap Q, Yip KY, Wong LZ, et al.
    Panminerva Med, 2021 Oct 05.
    PMID: 34609116 DOI: 10.23736/S0031-0808.21.04285-3
    According to the International Diabetes Federation, the number of adults (age of 20-79) being diagnosed with Diabetes Mellitus (DM) have increased from 285 million in year 2009 to 463 million in year 2019 which comprises of 95% Type 2 DM patient (T2DM). Research have claimed that genetic predisposition could be one of the factors causing T2DM complications. In addition, T2DMcomplications cause an incremental risk to mortality. Therefore, this article aims to discuss some complications of T2DM in and their genetic association. The complications that are discussed in this article are diabetic nephropathy, diabetes induced cardiovascular disease, diabetic neuropathy, Diabetic Foot Ulcer (DFU) and Alzheimer's disease. According to the information obtained, genes associated with diabetic nephropathy (DN) are gene GABRR1 and ELMO1 that cause injury to glomerular. Replication of genes FRMD3, CARS and MYO16/IRS2 shown to have link with DN. The increase of gene THBS2, NGAL, PIP, TRAF6 polymorphism, ICAM-1 encoded for rs5498 polymorphism and C667T increase susceptibility towards DN in T2DM patient. Genes associated with cardiovascular diseases are Adiponectin gene (ACRP30) and Apolipoprotein E (APOE) polymorphism gene with ξ2 allele. Haptoglobin (Hp) 1-1 genotype and Mitochondria Superoxide Dismutase 2 (SOD2) plays a role in cardiovascular events. As for genes related to diabetic neuropathy, Janus Kinase (JAK), mutation of SCN9A and TRPA1 gene and destruction of miRNA contribute to pathogenesis of diabetic neuropathy among T2DM patients. Expression of cytokine IL-6, IL-10, miR-146a are found to cause diabetic neuropathy. Besides, A1a16Va1 gene polymorphism, an oxidative stress influence was found as one of the gene factors. Diabetic retinopathy (DR) is believed to have association with Monocyte Chemoattractant Protein-1 (MCP-1) and Insulin-like Growth Factor 1 (IGF1). Over-expression of gene ENPP1, IL-6 pro-inflammatory cytokine, ARHGAP22's protein rs3844492 polymorphism and TLR4 heterozygous genotype are contributing to significant pathophysiological process causing DR, while research found increases level of UCP1 gene protects retina cells from oxidative stress. Diabetic Foot Ulcer (DFU) is manifested by slowing in reepithelialisation of keratinocyte, persistence wound inflammation and healing impairment. Reepithelialisation disturbance was caused by E2F3 gene, reduction of Tacl gene encoded substance P causing persistence inflammation while expression of MMp-9 polymorphism contributes to healing impairment. A decrease in HIF-1a gene expression leads to increased risk of pathogenesis, while downregulation of TLR2 increases severity of wound in DFU patients. SNPs alleles has been shown to have significant association between the genetic dispositions of T2DM and Alzheimer's disease (AD). The progression of AD can be due to the change in DNA methylation of CLOCK gene, followed with worsening of AD by APOE4 gene due to dyslipidaemia condition in T2DM patients. Insulin resistance is also a factor that contributes to pathogenesis of AD.
  6. Hassan IR, Omar D, Amit S, Ismail SI
    Plant Dis, 2021 Oct 05.
    PMID: 34609207 DOI: 10.1094/PDIS-04-21-0704-PDN
    Water hyacinth (Eichhornia crassipes) is a free-floating aquatic plant and is also widely cultivated as an aquatic ornamental plant in Malaysia. In June 2018, a severe foliar disease with typical leaf blight symptoms were observed on leaves of water hyacinth plants (approximately 50%) in waterways adjacent to two rice fields located at Tanjung Karang and Sungai Besar, Selangor province, Malaysia. Symptoms appeared irregular necrotic lesions with concentric rings, later lesions expanded to entire leaves and became blighted. Twenty symptomatic leaves were collected from two sampling locations. Symptomatic leaf tissue was cut into small pieces (5 × 5 mm), surface sterilized with 0.5% sodium hypochlorite (NaOCl) for 2 min, rinsed three times with sterile distilled water, plated on potato dextrose agar (PDA), and incubated at 25 °C with a 12-h light/dark cycle for 7 days. Twenty single-spore isolates were recovered from sampled leaves, all isolates exhibited Paramyrothecium-like morphology and two representative isolates, PR1 and PR2 were used for further studies. Fungal colonies were initially white aerial mycelia with sporodochia bearing olivaceous green conidial masses formed on PDA after 5 days of incubation. Conidiogenous cells were phialidic, hyaline, smooth, straight to slightly curved, 13 to 20 × 1.0 to 1.8 μm and setae were absent. Conidia were aseptate, hyaline to pale green, smooth, cylindrical to ellipsoidal with rounded ends, and measured 5.8 to 8.0 μm × 1.8 to 2.2 μm (n=50). These morphological characteristics were consistent with the description of Paramyrothecium roridum (Tode) L. Lombard & Crous (Lombard et al. 2016). Total genomic DNA of the isolates was extracted from fresh mycelium using DNeasy Plant Mini kit (Qiagen, USA). The internal transcribed spacer (ITS) and calmodulin (cmdA) gene regions were amplified using the ITS5/ITS4 (White et al.1990) and CAL-228F/CAL2Rd primer sets (Carbone and Kohn 1999; Groenewald et al., 2013), respectively. BLASTn analysis showed that the ITS and cmdA sequences of the isolates were 100% identity with Paramyrothecium roridum ex-epitype strain CBS 357.89 (GenBank accession nos. KU846300 and KU846270), respectively. The resulting sequences were deposited in GenBank (ITS: Accession nos. MW850370, MW850371; cmdA Accession nos. MW854363, MW854364). Pathogenicity tests of the two isolates were performed by spray inoculation on healthy leaves of each five potted water hyacinth plants using a 3-ml conidial suspension (1 × 106 conidia/ml) produced on 7-day-old PDA cultures incubated at 25 °C with a 12-h light/dark cycle. Five potted water hyacinth plants inoculated with sterile water served as controls. Inoculated plants were covered with plastic bags for 48 h to maintain high humidity and kept in a growth chamber for 2 weeks at 25 ± 1°C, 95% relative humidity and a 12-h light/dark period. The experiment was repeated twice. Eight days post-inoculation, symptoms on inoculated leaves developed necrotic brown lesions similar to those observed in the field, while control leaves remained asymptomatic. After 2 weeks of inoculation, lesions enlarged into severe blighting until all leaves died. Paramyrothecium roridum was re-isolated from randomly selected symptomatic tissues and verified by morphology and sequencing of ITS (MZ675387, MZ706462) and cmdA (MZ686706, MZ712041) loci, confirming Koch's postulates. The fungus was not re-isolated from non-inoculated control plants. Pa. roridum is distributed on a wide range of plants (Farr and Rossman 2021) and has been reported to cause leaf spot of water hyacinth in Nigeria (Okunowo et al. 2013) and Sri Lanka (Adikaram and Yakandawala 2020). To our knowledge, this is the first report of Pa. roridum causing leaf blight of water hyacinth in Malaysia. This disease is an emerging threat to water hyacinth and it reduces the leaf quality, therefore, appropriate management should be developed to control this disease.
  7. Arora R, Bansal V, Buckchash H, Kumar R, Sahayasheela VJ, Narayanan N, et al.
    Phys Eng Sci Med, 2021 Dec;44(4):1257-1271.
    PMID: 34609703 DOI: 10.1007/s13246-021-01060-9
    According to the World Health Organization (WHO), novel coronavirus (COVID-19) is an infectious disease and has a significant social and economic impact. The main challenge in fighting against this disease is its scale. Due to the outbreak, medical facilities are under pressure due to case numbers. A quick diagnosis system is required to address these challenges. To this end, a stochastic deep learning model is proposed. The main idea is to constrain the deep-representations over a Gaussian prior to reinforce the discriminability in feature space. The model can work on chest X-ray or CT-scan images. It provides a fast diagnosis of COVID-19 and can scale seamlessly. The work presents a comprehensive evaluation of previously proposed approaches for X-ray based disease diagnosis. The approach works by learning a latent space over X-ray image distribution from the ensemble of state-of-the-art convolutional-nets, and then linearly regressing the predictions from an ensemble of classifiers which take the latent vector as input. We experimented with publicly available datasets having three classes: COVID-19, normal and pneumonia yielding an overall accuracy and AUC of 0.91 and 0.97, respectively. Moreover, for robust evaluation, experiments were performed on a large chest X-ray dataset to classify among Atelectasis, Effusion, Infiltration, Nodule, and Pneumonia classes. The results demonstrate that the proposed model has better understanding of the X-ray images which make the network more generic to be later used with other domains of medical image analysis.
    MeSH terms: Algorithms; Humans; X-Rays; Neural Networks (Computer)
  8. Muthukumaran P, Suresh Babu P, Shyamalagowri S, Kamaraj M, Manikandan A, Aravind J
    J Basic Microbiol, 2021 Oct 05.
    PMID: 34609759 DOI: 10.1002/jobm.202100365
    The ever-rising environmental problems because of heavy metals emerging from anthropogenic activities pose an impending threat to all biota globally. Considering their persistence and possibility in biomagnification, they are prominent among pollutants. There has been an apparent shift of research interest in advancing cost-effective and competent technologies to mitigate environmental contaminants, specifically heavy metals. In the recent two decades, tailored nanomaterials (NMs), nanoparticles, and NM-based adsorbents have been emerging for removing heavy metal pollution on a sustainable scale, especially the green synthesis of these nanoproducts effective and nonhazardous means. Hence, this review explores the various avenues in nanotechnology, an attempt to gauge nanotechnological approaches to mitigate heavy metals in the aqueous system, especially emphasizing the recent trends and advancements. Inputs on remediating heavy metal in sustainable and environmentally benign aspects recommended future directions to compensate for the voids in this domain have been addressed.
  9. Yan L, Sunoj VSJ, Short AW, Lambers H, Elsheery NI, Kajita T, et al.
    New Phytol, 2021 12;232(6):2267-2282.
    PMID: 34610157 DOI: 10.1111/nph.17770
    Chilling restrains the distribution of mangroves. We tested whether foliar phosphorus (P) fractions and gene expression are associated with cold tolerance in mangrove species. We exposed seedlings of six mangrove populations from different latitudes to favorable, chilling and recovery treatments, and measured their foliar P concentrations and fractions, photochemistry, nighttime respiration, and gene expression. A Kandelia obovata (KO; 26.45°N) population completely and a Bruguiera gymnorhiza (Guangxi) (BGG; 21.50°N) population partially (30%) survived chilling. Avicennia marina (24.29°N), and other B. gymnorhiza (26.66°N, 24.40°N, and 19.62°N) populations died after chilling. Photosystems of KO and photosystem I of BGG were least injured. During chilling, leaf P fractions, except nucleic acid P in three populations, declined and photoinhibition and nighttime respiration increased in all populations, with the greatest impact in B. gymnorhiza. Leaf nucleic acid P was positively correlated with photochemical efficiency during recovery and nighttime respiration across populations for each treatment. Relatively high concentrations of nucleic acid P and metabolite P were associated with stronger chilling tolerance in KO. Bruguiera gymnorhiza exhibited relatively low concentrations of organic P in favorable and chilling conditions, but its partially survived population showed stronger compensation in nucleic acid P and Pi concentrations and gene expression during recovery.
    MeSH terms: China; Cold Temperature; Phosphorus; Photosynthesis; Plant Leaves; Avicennia*; Rhizophoraceae*
  10. Chen RE, Smith BK, Errico JM, Gordon DN, Winkler ES, VanBlargan LA, et al.
    Cell Host Microbe, 2021 Nov 10;29(11):1634-1648.e5.
    PMID: 34610295 DOI: 10.1016/j.chom.2021.09.006
    Although divergent dengue viruses (DENVs) have been isolated in insects, nonhuman primates, and humans, their relationships to the four canonical serotypes (DENV 1-4) are poorly understood. One virus isolated from a dengue patient, DKE-121, falls between genotype and serotype levels of sequence divergence to DENV-4. To examine its antigenic relationship to DENV-4, we assessed serum neutralizing and protective activity. Whereas DENV-4-immune mouse sera neutralize DKE-121 infection, DKE-121-immune sera inhibit DENV-4 less efficiently. Passive transfer of DENV-4 or DKE-121-immune sera protects mice against homologous, but not heterologous, DENV-4 or DKE-121 challenge. Antigenic cartography suggests that DENV-4 and DKE-121 are related but antigenically distinct. However, DENV-4 vaccination confers protection against DKE-121 in nonhuman primates, and serum from humans immunized with a tetravalent vaccine neutralize DENV-4 and DKE-121 infection equivalently. As divergent DENV strains, such as DKE-121, may meet criteria for serotype distinction, monitoring their capacity to impact dengue disease and vaccine efficacy appears warranted.
    MeSH terms: Animals; Antibodies, Viral/blood; Antibodies, Viral/immunology; Antigens, Viral/immunology; Cross Reactions; Dengue/immunology*; Dengue/prevention & control; Dengue/virology; Dengue Virus/genetics*; Dengue Virus/immunology*; Female; Genotype; Humans; Immunization, Passive; Macaca mulatta; Male; Mice, Inbred C57BL; Mice, Transgenic; Viral Envelope Proteins/immunology; Viral Envelope Proteins/chemistry; Mice; Dengue Vaccines/immunology*; Cross Protection*; Serogroup; Immunogenicity, Vaccine
  11. Hajar-Azhari S, Hafiz Abd Rahim M, Razid Sarbini S, Muhialdin BJ, Olusegun L, Saari N
    Food Res Int, 2021 11;149:110677.
    PMID: 34600679 DOI: 10.1016/j.foodres.2021.110677
    Fructooligosaccharides can be produced by direct enzymatic conversion from sucrose-rich sugarcane syrup (SS) consisting of 58.93% sucrose yielding 21.28 g FOS/100 g sucrose. This study evaluated the prebiotic effect of unpurified/purified SS containing FOS for the modulation of the human intestinal microbial composition and short-chain fatty acid production. The unpurified and purified FOS substrates, which were a mixture of 1-kestose, nystose and 1F-fructosylnystose, were supplemented into human faecal culture using a pH-controlled batch fermentation system and significantly increased the Bifidobacterium counts after 5 h fermentation, while Bacteroides/Prevotella counts were highest throughout 24 h fermentation. Meanwhile, Lactobacillus/Enterococcus exhibited a slight increase after 5 h fermentation before reaching a plateau afterwards. The steady Bacteroides/Prevotella growth and increased Bifidobacterium population promoted an increase in the production of short-chain fatty acids acetate (58 ± 2.70 mM), propionate (9.19 ± 5.94 mM) and butyrate (7.15 ± 2.28 mM). These results provide evidence that representative gut microbiota could utilise the enzymatically synthesised FOS to generate short-chain fatty acids as metabolites in pH-controlled conditions, thus FOS from SS are a potential prebiotic ingredient for foods and health drinks.
    MeSH terms: Gastrointestinal Microbiome*; Fatty Acids, Volatile; Feces; Humans; Oligosaccharides; Saccharum*
  12. Yong MY, Tan KY, Tan CH
    Toxicon, 2021 Nov;203:85-92.
    PMID: 34600909 DOI: 10.1016/j.toxicon.2021.09.021
    The Trimeresurus complex consists of diverse medically important venomous pit vipers that cause snakebite envenomation. Antivenoms, however, are in limited supply, and are specific to only two out of the many species across Asia. This study thus investigated the immunoreactivities of regional pit viper antivenoms toward selected Trimeresurus pit viper venoms, and examined the neutralization of their hemotoxic activities. Trimeresurus albolabris Monovalent Antivenom (TaMAV, Thailand) exhibited a higher immunoreactivity than Hemato Bivalent Antivenom (HBAV, raised against Trimeresurus stejnegeri and Protobothrops mucrosquamatus, Taiwan) and Gloydius brevicaudus Monovalent Antivenom (GbMAV, China), attributed to its monovalent nature and conserved antigens in the Trimeresurus pit viper venoms. The venoms showed moderate-to-strong in vitro procoagulant and in vivo hemorrhagic effects consistent with hemotoxic envenomation, except for the Sri Lankan Trimeresurus trigonocephalus venom which lacked hemorrhagic activity. TaMAV was able to differentially neutralize both in vitro and in vivo hemotoxic effects of the venoms, with the lowest efficacy shown against the procoagulant effect of T. trigonocephalus venom. The findings suggest that TaMAV is a potentially useful treatment for envenomation caused by hetero-specific Trimeresurus pit vipers, in particular those in Southeast Asia and East Asia. Clinical study is warranted to establish its spectrum of para-specific effectiveness, and dosages need be tailored to the different species in respective regions.
    MeSH terms: Animals; Antivenins; Snake Bites*; Thailand; Viper Venoms; Trimeresurus*
  13. Teng ST, Abdullah N, Hanifah AH, Tan SN, Gao C, Law IK, et al.
    Toxicon, 2021 Sep 30;202:132-141.
    PMID: 34600910 DOI: 10.1016/j.toxicon.2021.09.018
    In March 2018, an algal bloom of Pseudo-nitzschia was detected, for the first time, in a semi-enclosed lagoon in Miri, Sarawak, Malaysia Borneo. The plankton samples were collected for cell enumeration and species identification by electron microscopy and molecular characterization. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was performed to detect and quantify the neurotoxin domoic acid (DA) in both the plankton and shellfish samples. The abundance of Pseudo-nitzschia cells ranged from 5.6 × 105 to 3.5 × 106 cell L-1 during the bloom event. Morphological observation of the cells by transmission electron microscopy showed that the plankton samples comprised a single Pseudo-nitzschia morphotype resembling P. cuspidata. The ITS2 sequence-structure phylogenetic inference further supported the species identity as Pseudo-nitzschia cuspidata. Low levels of DA were detected in the plankton samples, with cellular DA, particulate DA, and dissolved DA of 257-504 fg DA cell-1, 676 ng L-1, and 15 ng L-1, respectively. The amount of DA, 8 μg g-1 tissue, was found present in the shellfish sample (Magallana sp.) which is below the regulatory limit of 20 μg DA g-1 tissue. The study documented, for the first time, DA contamination in shellfish that associated with bloom of P. cuspidata in the Western Pacific region.
  14. Sadiq AC, Olasupo A, Ngah WSW, Rahim NY, Suah FBM
    Int J Biol Macromol, 2021 Nov 30;191:1151-1163.
    PMID: 34600954 DOI: 10.1016/j.ijbiomac.2021.09.179
    The presence of dyes in the aquatic environment as a result of anthropogenic activities, especially textile industries, is a critical environmental challenge that hinders the availability of potable water. Different wastewater treatment approaches have been used to remediate dyes in aquatic environments; however, most of these approaches are limited by factors ranging from high cost to the incomplete removal of the dyes and contaminants. Thus, the use of adsorption as a water treatment technology to remove dyes and other contaminants has been widely investigated using different adsorbents. This study evaluated the significance of chitosan as a viable adsorbent for removing dyes from water treatment. We summarised the literature and research results obtained between 2009 and 2020 regarding the adsorption of dyes onto chitosan and modified chitosan-based adsorbents prepared through physical and chemical processing, including crosslinking impregnation, grafting, and membrane preparation. Furthermore, we demonstrated the effects of various chitosan-based materials and modifications; they all improve the properties of chitosan by promoting the adsorption of dyes. Hence, the application of chitosan-based materials with various modifications should be considered a cutting-edge approach for the remediation of dyes and other contaminants in aquatic environments toward the global aim of making potable water globally available.
    MeSH terms: Adsorption; Coloring Agents/chemistry*; Solvents; Textile Industry; Waste Disposal, Fluid/methods; Water Pollutants, Chemical/chemistry; Water Purification/methods*; Chitosan/chemistry*; Nanoparticles; Nanocomposites; Waste Water
  15. Oh S, Lee J, Lam SS, Kwon EE, Ha JM, Tsang DCW, et al.
    Bioresour Technol, 2021 Dec;342:126067.
    PMID: 34601023 DOI: 10.1016/j.biortech.2021.126067
    Recent studies show that fast hydropyrolysis (i.e., pyrolysis under hydrogen atmosphere operating at a rapid heating rate) is a promising technology for the conversion of biomass into liquid fuels (e.g., bio-oil and C4+ hydrocarbons). This pyrolysis approach is reported to be more effective than conventional fast pyrolysis in producing aromatic hydrocarbons and also lowering the oxygen content of the bio-oil obtained compared to hydrodeoxygenation (a common bio-oil upgrading method). Based on current literature, various non-catalytic and catalytic fast hydropyrolysis processes are reviewed and discussed. Efforts to combine fast hydropyrolysis and hydrotreatment process are also highlighted. Points to be considered for future research into fast hydropyrolysis and pending challenges are also discussed.
  16. Kho ASK, Ooi EH, Foo JJ, Ooi ET
    Comput Methods Programs Biomed, 2021 Nov;211:106436.
    PMID: 34601185 DOI: 10.1016/j.cmpb.2021.106436
    BACKGROUND AND OBJECTIVE: Saline infusion is applied together with radiofrequency ablation (RFA) to enlarge the ablation zone. However, one of the issues with saline-infused RFA is backflow, which spreads saline along the insertion track. This raises the concern of not only thermally ablating the tissue within the backflow region, but also the loss of saline from the targeted tissue, which may affect the treatment efficacy.

    METHODS: In the present study, 2D axisymmetric models were developed to investigate how saline backflow influence saline-infused RFA and whether the aforementioned concerns are warranted. Saline-infused RFA was described using the dual porosity-Joule heating model. The hydrodynamics of backflow was described using Poiseuille law by assuming the flow to be similar to that in a thin annulus. Backflow lengths of 3, 4.5, 6 and 9 cm were considered.

    RESULTS: Results showed that there is no concern of thermally ablating the tissue in the backflow region. This is due to the Joule heating being inversely proportional to distance from the electrode to the fourth power. Results also indicated that larger backflow lengths led to larger growth of thermal damage along the backflow region and greater decrease in coagulation volume. Hence, backflow needs to be controlled to ensure an effective treatment of saline-infused RFA.

    CONCLUSIONS: There is no risk of ablating tissues around the needle insertion track due to backflow. Instead, the risk of underablation as a result of the loss of saline due to backflow was found to be of greater concern.

    MeSH terms: Electrodes; Liver/surgery; Porosity; Catheter Ablation*
  17. Xu XJ, Myint PK, Kioh SH, Mat S, Rajasuriar R, Kamaruzzaman SB, et al.
    Arch Gerontol Geriatr, 2021 09 30;98:104535.
    PMID: 34601313 DOI: 10.1016/j.archger.2021.104535
    BACKGROUND: While anticholinergic use is associated with stroke, dementia and mortality, few have evaluated its potential link with falls. To determine the relationship between anticholinergic cognitive burden (ACB) and falls over five years using the Malaysian Elders Longitudinal Research (MELoR).

    METHODS: Community-dwelling adults aged 55 years and over were recruited through electoral roll sampling. Data obtained at baseline and follow-up (FU) at two and five years were included. Falls in the preceding 12 months were recorded.

    RESULTS: Of the 1499 individuals (mean (SD) age= 68.9(7.5) yrs and 53.3% female) with information on baseline ACB exposure, 575(38.4%) had ACB scores of 1-2 and 117(7.8%) had ACB scores ≥3. Differences in age, ethnicity, smoking status, diabetes, hypertension, cardiovascular disease, arthritis and education existed between ACB groups. Fall occurrence differed between ACB groups at recruitment (p = 0.004) and 2-year FU (p = 0.001) but not at 5-year FU (p = 0.053). Logistic regression revealed an independent association between ACB 1-2 and falls at baseline (odds ratio, OR (95% confidence interval, CI) =1.412(1.035-1.926)) and ACB≥3 and falls at 2-yr FU (OR (95%CI) =2.098(1.032-4.263)) following adjustment for confounders.

    CONCLUSION: Low level exposure to drugs with anticholinergic properties was associated cross-sectionally with falls, while exposure to higher levels were prospectively associated with falls at 2-year but not at 5-year FU. Future studies should determine whether avoidance of drugs with anticholinergic effects will lead to reduction in falls.

  18. Shankar PR
    J Nepal Health Res Counc, 2021 Sep 06;19(2):439-440.
    PMID: 34601549 DOI: 10.33314/jnhrc.v19i2.3339
    Medical humanities use the creative and intellectual strengths of the arts for specific purposes in medical education. The author read with great interest the Medical humanities program at the Patan Academy of Health Sciences and has been associated with Medical humanities since 2007. There are several factors favouring the development of Medical humanities in Nepal but the major challenge is there is no specific faculty and/or department involved and the discipline may be relegated to the background in the face of other pressing priorities. In the west humanities faculty had played an important role in popularizing Medical humanities. Clinical teachers can incorporate Medical humanities into their clinical teaching. Large student sizes and lesser number of faculty may be challenges in moving the discipline forward. Keywords: Medical humanities, medical schools, Nepal, undergraduate medical.
    MeSH terms: Education, Medical*; Humans; Humanities; Medicine*; Nepal; Schools, Medical
  19. Malik AS, Malik RH
    J Coll Physicians Surg Pak, 2021 Oct;31(10):1141-1142.
    PMID: 34601830 DOI: 10.29271/jcpsp.2021.10.1141
    Null.
    MeSH terms: Education, Medical*; Humans
  20. Rajendran MAP, Allada R, Sajid SS
    Recent Adv Drug Deliv Formul, 2021;15(1):15-36.
    PMID: 34602030 DOI: 10.2174/2667387815666210203151209
    Co-crystal is an attractive alternative and a new class of solid forms because that can be engineered to have desired physicochemical properties. Co-crystals have gained considerable attention from the generic pharmaceutical industry after the USFDA released its finalized guidlines in the year 2018 on the regulatory classification of co-crystals. In this review, we discussed how co-crystals could be explored as a potential alternative solid form for the development of a generic product that meets the legal, regulatory, and bioequivalence requirements. In the contents, we discussed in detail concepts such as the selection of coformers, various ways of making co-crystals, the strategy of characterization to discriminate between co-crystal and salt, polymorphism in co-crystals, the aspects of intellectual property and, finally, the regulatory aspects of co-crystals.
    MeSH terms: Drug Compounding; Therapeutic Equivalency; Drugs, Generic*
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