Browse publications by year: 2021

  1. Behrooz AB, Vazifehmand R, Tajudin AA, Masarudin MJ, Sekawi Z, Masomian M, et al.
    Drug Deliv Transl Res, 2021 Aug 17.
    PMID: 34405338 DOI: 10.1007/s13346-021-01017-1
    Glioblastoma multiforme (GBM) is the most prevalent form of brain tumor, which generally has a poor prognosis. According to consensus, recurrence of the tumor and chemotherapy resistance acquisition are the two distinguishing features of GBM originated from glioblastoma stem cells (GSCs). To eliminate these obstacles inherent in GBM chemotherapy, targeting GSCs through a smart drug delivery system has come to the front position of GBM therapeutics. In this study, B19 aptamer (Apt)-conjugated polyamidoamine (PAMAM) G4C12 dendrimer nanoparticles (NPs), called Apt-NPs, were formulated for the co-delivery of paclitaxel (PTX) and temozolomide (TMZ) to U-87 stem cells. These drugs were loaded using a double emulsification solvent evaporation method. As a result, drug-loaded Apt-NPs significantly inhibited the tumor growth of U-87 stem cells, by the initiation of apoptosis via the downregulation of autophagic and multidrug resistance (MDR) genes. Additionally, by their downregulation by qPCR of CD133, CD44, SOX2, and the canonical Wnt/β-catenin pathway, cell proliferation has substantially decreased. Altogether, the results demonstrate that this intelligent drug co-delivery system is capable of effectively transferring PTX and TMZ to U-87 stem cells and without any toxic effect on Apt-NPs alone to U-87 stem cells. Furthermore, the designed dendrimer-based pharmaceutical system along with single-stranded B19 aptamer might be utilized as a new therapeutic strategy for the treatment of U-87 stem cells drug resistance in the GBM.
  2. Mohd Hanim MF, Md Sabri BA, Yusof N
    JMIR Public Health Surveill, 2021 08 18;7(8):e24523.
    PMID: 34406125 DOI: 10.2196/24523
    BACKGROUND: In Malaysia, the Sugar-Sweetened Beverages (SSBs) tax was announced during the parliament's 2019 Budget Speech. The tax was slated to be enforced by April 2019 but was later postponed to July 2019. The announcement has since generated significant media coverage and public feedback.

    OBJECTIVE: This study presents a qualitative and quantitative cross-sectional study using netnography to examine how Malaysian online news articles responded to the SSBs tax after the announcement and postimplementation.

    METHODS: Online news articles published on popular online news platforms from November 2018 to August 2019 were downloaded using NCapture and imported into NVivo for analysis using the inductive approach and thematic content analysis following the initial SSBs implementation announcement.

    RESULTS: A total of 62 news articles were analyzed. Most of the articles positively portrayed the SSBs tax (46.8%) and highlighted its health impacts (76%). There were 7 key framing arguments identified in the articles. The positive arguments revolved around incentivizing manufacturers to introduce healthier products voluntarily, positive health consequences, the tax's impact on government revenue, and the use of the generated revenue toward beneficial social programs. The opposing arguments included increased operating costs to the manufacturer, the increased retail price of drinks, and how the SSBs tax is not a robust solution to obesity. The top priority sector considered in introducing the tax was the health perspective, followed by economic purposes and creating policies such as regulating the food and drinks industry.

    CONCLUSIONS: The majority of online news articles positively reported the implementation of the SSBs tax in Malaysia. This suggests media played a role in garnering support for the health policy. As such, relevant bodies can use negative findings to anticipate and reframe counteracting arguments opposing the SSBs tax.

    MeSH terms: Cross-Sectional Studies; Humans; Malaysia; Mass Media*; Taxes*; Internet
  3. Kountouris P, Stephanou C, Archer N, Bonifazi F, Giannuzzi V, Kuo KHM, et al.
    Am J Hematol, 2021 Nov 01;96(11):E416-E420.
    PMID: 34406671 DOI: 10.1002/ajh.26323
    MeSH terms: Anemia, Sickle Cell/genetics; Hemoglobinopathies/genetics*; Humans; Genetic Variation; Databases, Factual; Genetic Predisposition to Disease
  4. Siti Nur Ain Mustafa, Mohamad Saifudin Mohamad Saleh, Azman Azwan Azmawati
    MyJurnal
    Bencana tsunami ini sememangnya memberikan kesan terhadap kesejahteraan ekonomi dan sosial negara. Di antara negara-negara Asia Tenggara, Indonesia dikenal pasti sebagai salah sebuah negara yang sering menghadapi bencana tsunami. Malaysia yang kedudukannya berhampiran dengan Indonesia turut menerima kesan daripada bencana tsunami yang terjadi di negara berkenaan. Kejadian bencana tsunami yang sememangnya serius ini telah mendapat pelaporan yang meluas oleh media tempatan dan antarabangsa. Bencana tsunami yang lazimnya berlaku secara tiba-tiba dan sukar untuk dijangka ini telah menyebabkan pelaporannya berbeza dengan isu-isu lain seperti jenayah, hiburan dan sebagainya. Malah, kajian-kajian lalu turut mendapati bahawa media menghadapi pelbagai cabaran dalam melaporkan berita bencana seperti tsunami yang jelas memerlukan persediaan yang rapi bagi menghasilkan penulisan yang berkualiti dan beretika. Sungguhpun kejadian tsunami adalah serius, kajian-kajian lalu terhadap pelaporan media khususnya dalam aspek cabaran wartawan dalam melaporkan isu ini adalah amat terhad. Justeru, kajian ini dijalankan dengan matlamat untuk memenuhi kelompongan kajian lepas dan seterusnya menyumbang kepada korpus penyelidikan bidang Komunikasi Bencana di Malaysia dan Indonesia. Penyelidikan ini memberikan tumpuan terhadap cabaran-cabaran yang dihadapi oleh para wartawan kedua-dua negara dalam melaporkan berita tsunami. Temu bual secara mendalam telah dilakukan bersama lapan orang wartawan dari The Star Online, Malaysia dan The Jakarta Post, Indonesia. Hasil kajian mendapati bahawa bilangan wartawan pakar isu terhad, pengetahuan terhad wartawan tentang bencana tsunami, kurang persediaan oleh bilik berita, pemotretan visual ketika bencana dan penentuan landskap berita oleh editor adalah cabaran-cabaran utama yang dihadapi oleh wartawan-wartawan di Malaysia dan Indonesia dalam melaporkan isu tsunami.
  5. NOR ZALILAH MOHD NORDIN, MOHAMED KAMEL ABD GHANI, YUSOF SUBOH, NORAINA AB RAHIM, ANISAH NORDIN
    Buletin Sains Kesihatan, 2021;5(1):30-35.
    MyJurnal
    Acanthamoeba is a free-living amoeba that can cause serious eye infection leading to blindness known as Acanthamoeba keratitis. The infection usually occurs amongst individuals wearing contact lenses. The use of contact lens disinfecting solution not effective against the Acanthamoeba cysts increases the risk for infection. There have been many studies indicating the resistance of Acanthamoeba cyst towards certain antimicrobial agents, enzymatic disinfectant and chemical disinfectant. Therefore, this study was performed to evaluate the efficacy of contact lens disinfecting solutions comprising Opti-Free® Express® & Complete™, against the cysts of Acanthamoeba. This study also investigates the effectiveness of the disinfecting solutions based on the soaking time recommended by manufacturers, 4 hours, 6 hours and 8 hours. Four clinical isolates of Acanthamoeba were tested in this study comprising HKL 11, HKL 51, HKL 32 and HKL 5. Ten microliter of cysts suspension (105 cells/ml) was tested on the disinfectant solution respectively. After an interval varying from 4 hours to 8 hours, the solutions were centrifuged and then the sediment was inoculated onto non nutrient agar lawn with E. coli. The plates were examined daily using inverted microscope for the emergence of Acanthamoeba trophozoites. The presence of Acanthamoeba trophozoites indicates that the cysts had successfully undergone excystation process denoting that the disinfecting solution is not effective. Opti-Free® Express® & Complete™ contact lens disinfecting solutions were not effective against all 4 isolates of Acanthamoeba cysts based on time recommended by manufacturers, 4 hours, 6 hours and even 8 hours soaking time. This study shows that there are more contact lens disinfecting solutions not effective against Acanthamoeba cysts compared to those that are effective.
  6. MOHD NAQIUDDIN JOHAR, NOR AZLIN MOHD NORDIN, YUSLIZA AZREEN
    Buletin Sains Kesihatan, 2021;5(1):21-29.
    MyJurnal
    Circuit exercise therapy is being frequently used as a therapy option for stroke patients undergoing rehabilitation. However, the effectiveness of this therapy in improving post-stroke functionality remains limited. There is also a need to improvise the content of the existing circuit exercise therapy to further enhance the process of neuroplasticity towards the attainment of greater recovery. One strategy for this is to integrate the element of games into the exercise therapy to create a more enriched training environment during the exercise session. This study aimed to determine the feasibility and effectiveness of a game-based circuit exercise therapy among stroke survivors. This was a pilot experimental study involving 30 stroke patients at post-acute phase post-onset (mean age and standard deviation = 58.9 ± 6.6 years; mean Montreal Cognitive Assessment score = 23.4 ± 7.1) and conducted in a district of Kelantan, Malaysia. Simple random sampling method was used in recruiting the study participants. All participants undergone game-based circuit exercise therapy using a game board named “Checkercise” for 45 minutes, twice per week for 8 weeks. The feasibility of the therapy was measured in term of attrition rate, occurrence of adverse effects and feedbacks from the participants. While its effectiveness on post-stroke physical function were assessed with regards to improvement in functional stability and cardiovascular endurance, using Dynamic Gait Index (DGI) and 6-minute Walk Test, respectively. All data were analyzed using the paired samples t test, with the level of significance, p set at < 0.05. Study results shown low attrition rate, no apparent adverse effect, and positive feedbacks from the participants. Improvement in the participants’ mean score for DGI and 6-minute walk test by 7% and 23% respectively (p < 0.05), and medium effect size (Cohen’s d= 0.5 - 0.6) was obtained post-therapy. In conclusion, game-based circuit exercise therapy is feasible and beneficial for stroke survivors. The therapy can potentially be used as a therapy option for this population. A larger study is needed to confirm this study finding.
  7. AIMI NAJWA OSMERA, FAZLEEN HASLINDA MOHD HATTA, HARTINI YUSOF
    Buletin Sains Kesihatan, 2021;5(1):11-20.
    MyJurnal
    In the 21st century, obesity and its related health complications have become a serious problem worldwide. The primary reasons for this situation are environmental and genetic factors. In this review, we focused on a genetic marker that was found to play a crucial role in determining an individual’s predisposition to weight gain and being obese. This dissertation will also have a look into genome-wide association studies (GWAS) that revealed the possible link of a single nucleotide polymorphism (SNPs) in the first intron of the Fat Mass Obesity Associated (FTO) gene to body mass index and obesity. Moreover, it has become clear that this association with the regulation of body weight is replicable across multiple populations. Furthermore, evidence from animal models where FTO expression has indicated the role of the FTO gene in the regulation of body weight. In the regulation of body weight, FTO protein disallows weight gain by increasing the body’s sensitivity to satiety to prevent excessive food intake through fat cell lipolysis. On the other hand, the FTO variant risk allele increased the appetite and energy intake, but not the expenditure of energy that can lead to weight gain. Individual that is having homozygous of the risk allele, which is the A-allele, was associated with the risk of being obese and the rise in BMI. The purpose of this review is to discuss the association of the FTO gene common variant, rs9939609 with obesity and body mass index, and to describe the role of the FTO gene in the regulation of body weight.
  8. AHMAD ZORIN SAHALAN
    Buletin Sains Kesihatan, 2021;5(1):7-10.
    MyJurnal
    Etilingera elatior or kantan is from the Zingiberaceae family which has a variety of nourishments. In this experiment, the flower buds were used to extract non-polar compound using petroleum ether solvents. Kantan extract is tested for its antimicrobial property against skin bacteria where this test helps to see evaluate its role in fighting skin infection. In addition it is also tested on fungi such as Candida albicans and Asperigillus niger. Our results showed that it inhibits bacteria, namely Staphylococcus aureus, Staphylococcus epidemidis and Streptococcus feacalis. In addition, non-polar kantan extract also inhibits the growth of fungi such as C. albicans and A. niger. In conclusion this extract has the potential in medical treatment and is a broad spectrum compound.
  9. SHAHIRAH, S., MOHAMED KAMEL, A. G., MAZLAN, M., NUR HAZIQAH, O., NUR EMILIA, O., NUR ATHIKAH, J., et al.
    Buletin Sains Kesihatan, 2021;5(1):1-6.
    MyJurnal
    Acanthamoeba is a free-living amoeba and opportunistic protozoa that causes infection to humans. One of its primary infections is Acanthamoeba keratitis that occurs mostly amongst contact lens users. There are some difficulties in providing effective treatment to the patients as the cysts of Acanthamoeba spp. are very resistant towards most of the antimicrobial agents. Thus, study was conducted to determine the effectiveness of antimicrobial agents comprising 0.1% propamidine isethionate (Brolene®) and 0.3% ciprofloxacin (Ciloxan®) towards two clinical isolates of Acanthamoeba spp. (HUKM 74 and HS 72). Besides that, the minimum cysticidal concentration (MCC) of the tested antimicrobial agents were also determined. Propamidine isethionate and ciprofloxacin showed cysticidal activities at therapeutic dosage against both Acanthamoeba spp. isolates. The minimum cysticidal concentration for propamidine isethionate was between 62.5-125 µg/ml while for ciprofloxacin was 375 µg/ml. Both the clinical isolates of Acanthamoeba belonged to group II (Polyphagids).
  10. Origomisan JO, Lau WJ, Aziz F, Ismail AF
    Recent Pat Nanotechnol, 2021;15(2):105-124.
    PMID: 33059573 DOI: 10.2174/1872210514666201014152621
    Polyamide (PA) Thin-Film Composite (TFC) membranes are widely used for large-scale water and wastewater treatment processes worldwide owing to their good balance between water permeability and dissolved solutes separation rate. The physicochemical properties of the cross-linked PA layer are the main criteria determining the filtration performance of the resultant TFC membrane, and this selective layer can be created through Interfacial Polymerization (IP) between two immiscible active monomers, i.e., amine monomer in aqueous solution and acyl chloride monomer in organic solution. This patent review article intends to provide insights to researchers in fabricating improved properties of TFC membranes through the utilization of secondary monomers during IP process. To the best of our knowledge, this is the first review that gives a state-of-the-art account of the subject matter by emphasizing the impacts of secondary monomers (both amine and acyl chloride monomers) on the properties of conventional TFC membranes for nanofiltration and reverse osmosis applications. Our review indicated that the introduction of secondary monomers into either aqueous or organic solution could alter the physical and chemical properties of PA layer, which led to variations in membrane filtration performance. Nevertheless, more research is still required, as most of the secondary monomers reported in the literature did not overcome the membrane trade-off effect between permeability and selectivity. The subject of improved PA layer development is a multi-disciplinary study that requires researchers with different backgrounds (e.g., materials science, chemistry, physics and engineering) to work together.
  11. Perak AM, Lancki N, Kuang A, Labarthe DR, Allen NB, Shah SH, et al.
    JAMA, 2021 02 16;325(7):658-668.
    PMID: 33591345 DOI: 10.1001/jama.2021.0247
    Importance: Pregnancy may be a key window to optimize cardiovascular health (CVH) for the mother and influence lifelong CVH for her child.

    Objective: To examine associations between maternal gestational CVH and offspring CVH.

    Design, Setting, and Participants: This cohort study used data from the Hyperglycemia and Adverse Pregnancy Outcome (HAPO) Study (examinations: July 2000-April 2006) and HAPO Follow-Up Study (examinations: February 2013-December 2016). The analyses included 2302 mother-child dyads, comprising 48% of HAPO Follow-Up Study participants, in an ancillary CVH study. Participants were from 9 field centers across the United States, Barbados, United Kingdom, China, Thailand, and Canada.

    Exposures: Maternal gestational CVH at a target of 28 weeks' gestation, based on 5 metrics: body mass index, blood pressure, total cholesterol level, glucose level, and smoking. Each metric was categorized as ideal, intermediate, or poor using pregnancy guidelines. Total CVH was categorized as follows: all ideal metrics, 1 or more intermediate (but 0 poor) metrics, 1 poor metric, or 2 or more poor metrics.

    Main Outcomes and Measures: Offspring CVH at ages 10 to 14 years, based on 4 metrics: body mass index, blood pressure, total cholesterol level, and glucose level. Total CVH was categorized as for mothers.

    Results: Among 2302 dyads, the mean (SD) ages were 29.6 (2.7) years for pregnant mothers and 11.3 (1.1) years for children. During pregnancy, the mean (SD) maternal CVH score was 8.6 (1.4) out of 10. Among pregnant mothers, the prevalence of all ideal metrics was 32.8% (95% CI, 30.6%-35.1%), 31.7% (95% CI, 29.4%-34.0%) for 1 or more intermediate metrics, 29.5% (95% CI, 27.2%-31.7%) for 1 poor metric, and 6.0% (95% CI, 3.8%-8.3%) for 2 or more poor metrics. Among children of mothers with all ideal metrics, the prevalence of all ideal metrics was 42.2% (95% CI, 38.4%-46.2%), 36.7% (95% CI, 32.9%-40.7%) for 1 or more intermediate metrics, 18.4% (95% CI, 14.6%-22.4%) for 1 poor metric, and 2.6% (95% CI, 0%-6.6%) for 2 or more poor metrics. Among children of mothers with 2 or more poor metrics, the prevalence of all ideal metrics was 30.7% (95% CI, 22.0%-40.4%), 28.3% (95% CI, 19.7%-38.1%) for 1 or more intermediate metrics, 30.7% (95% CI, 22.0%-40.4%) for 1 poor metric, and 10.2% (95% CI, 1.6%-20.0%) for 2 or more poor metrics. The adjusted relative risks associated with 1 or more intermediate, 1 poor, and 2 or more poor (vs all ideal) metrics, respectively, in mothers during pregnancy were 1.17 (95% CI, 0.96-1.42), 1.66 (95% CI, 1.39-1.99), and 2.02 (95% CI, 1.55-2.64) for offspring to have 1 poor (vs all ideal) metrics, and the relative risks were 2.15 (95% CI, 1.23-3.75), 3.32 (95% CI,1.96-5.62), and 7.82 (95% CI, 4.12-14.85) for offspring to have 2 or more poor (vs all ideal) metrics. Additional adjustment for categorical birth factors (eg, preeclampsia) did not fully explain these significant associations (eg, relative risk for association between 2 or more poor metrics among mothers during pregnancy and 2 or more poor metrics among offspring after adjustment for an extended set of birth factors, 6.23 [95% CI, 3.03-12.82]).

    Conclusions and Relevance: In this multinational cohort, better maternal CVH at 28 weeks' gestation was significantly associated with better offspring CVH at ages 10 to 14 years.

    MeSH terms: Child Health*; Adolescent Health*; Maternal Health*; Adolescent; Adult; Cardiovascular Diseases/epidemiology; Cardiovascular Diseases/prevention & control; Cardiovascular System*; Child; Female; Humans; Male; Pregnancy*; Cohort Studies; Health Behavior; Prevalence
  12. Zhang C, Ho SH, Chen WH, Wang R, Show PL, Ong HC
    J Biotechnol, 2021 Sep 10;338:81-90.
    PMID: 34298023 DOI: 10.1016/j.jbiotec.2021.07.009
    Microalgae are a promising feedstock for carbon-neutral biofuel production due to their superior cellular composition. Alternatively, oxidative torrefaction has been recognized as a potential thermochemical technique for microalgal solid biofuel upgrading. Herein, by using microalga N. oceanica as a feedstock, several characterizations are adopted for evaluating the potential of oxidative torrefaction towards microalgal solid biofuel production. The oxidatively torrefied microalgae can be upgraded as lignite. After in-depth analysis, significant change in the surface microstructure of oxidatively torrefied microalgae is largely changed (via wrinkle and fragmentation) The hydrophobicity, thermal decomposition, thermal stability, and aromatization of oxidatively torrefied microalgae can be largely enhanced as the oxidative torrefaction severity increase. With the increasing torrefaction temperature, the hydrophobicity of oxidative torrefied microalgae gradually improved. The decomposition of C-2/3/5, and -OCH3, the CO bonds of CH3CO-, and the aromatization occurs via oxidative torrefaction according to the NMR analysis. For XPS analysis, torrefaction operation significantly decreases the carbide carbon and enhances the graphitization. As a result, the thermal stability of oxidatively torrefied microalgae is improved. Conclusively, the information obtained in this study can provide insights into the evaluation of oxidative torrefaction performance and fuel properties of microalgal solid biofuel, which may help accelerate the advancement of oxidative torrefaction industrialization.
    MeSH terms: Temperature; Oxidative Stress; Biomass; Biofuels; Stramenopiles*; Microalgae*
  13. Koh W, Chakravarthy M, Simon E, Rasiah R, Charuluxananan S, Kim TY, et al.
    BMC Anesthesiol, 2021 08 16;21(1):205.
    PMID: 34399681 DOI: 10.1186/s12871-021-01414-6
    BACKGROUND: Anesthesia leads to impairments in central and peripheral thermoregulatory responses. Inadvertent perioperative hypothermia is hence a common perioperative complication, and is associated with coagulopathy, increased surgical site infection, delayed drug metabolism, prolonged recovery, and shivering. However, surveys across the world have shown poor compliance to perioperative temperature management guidelines. Therefore, we evaluated the prevalent practices and attitudes to perioperative temperature management in the Asia-Pacific region, and determined the individual and institutional factors that lead to noncompliance.

    METHODS: A 40-question anonymous online questionnaire was distributed to anesthesiologists and anesthesia trainees in six countries in the Asia-Pacific (Singapore, Malaysia, Philippines, Thailand, India and South Korea). Participants were polled about their current practices in patient warming and temperature measurement across the preoperative, intraoperative and postoperative periods. Questions were also asked regarding various individual and environmental barriers to compliance.

    RESULTS: In total, 1154 valid survey responses were obtained and analyzed. 279 (24.2%) of respondents prewarm, 508 (44.0%) perform intraoperative active warming, and 486 (42.1%) perform postoperative active warming in the majority of patients. Additionally, 531 (46.0%) measure temperature preoperatively, 767 (67.5%) measure temperature intraoperatively during general anesthesia, and 953 (82.6%) measure temperature postoperatively in the majority of patients. The availability of active warming devices in the operating room (p 

    MeSH terms: Asia; Body Temperature*; Cross-Sectional Studies; Humans; Hypothermia/prevention & control*; Intraoperative Complications/prevention & control; Practice Patterns, Physicians'/statistics & numerical data*; Surveys and Questionnaires; Monitoring, Intraoperative*; Perioperative Care*; Anesthesiologists
  14. Loh P, Chua SS, Karuppannan M
    BMC Health Serv Res, 2021 Aug 16;21(1):822.
    PMID: 34399749 DOI: 10.1186/s12913-021-06820-7
    BACKGROUND: Since the introduction of pharmaceutical care concept by Hepler and Strand in 1990, community pharmacists worldwide have been realigning their roles from being product-focused to patient-orientated to improve patient's quality of life. The objectives of this study were to determine the type of services, with emphasis on the extent of pharmaceutical care services provided by community pharmacists and the barriers in providing such services in Malaysia.

    METHODS: A cross-sectional observational study was conducted using an online questionnaire. Community pharmacists in Malaysia were invited to participate in the study via emails. The questionnaire was structured based on the Malaysian Community Pharmacy Benchmarking Guidelines, the five practice principles of pharmaceutical care by the American Pharmacists Association and other studies. The online questionnaire was opened for 6 months, from April to September 2018. A reminder to participate was sent via email to the community pharmacists every fortnight.

    RESULTS: A total of 420 community pharmacists responded to the online questionnaire. Besides essential services such as treatment for minor illness, medicine dispensing and counselling, most of the respondents were providing health screening and monitoring (99.5%), selection and recommendation of health supplements (90.5%), patient medication review (68.8%), weight management (52.4%) and counselling on smoking cessation (51.0%). More than half (53.3%) of the respondents reported that they were providing pharmaceutical care services to patients with chronic diseases. Based on the practice principles of pharmaceutical care, the respondents were involved in patients' data collection (23.3%), medical information evaluation (18.6%), formulating a drug therapy plan (9.3%), implementing a drug therapy plan (4.5%), and monitoring and modifying the plan (18.3%). Lack of separation between prescribing and dispensing was perceived as the main barrier to the implementation of pharmaceutical care services by a majority of the respondents (84.0%).

    CONCLUSIONS: The present study found that pharmaceutical care services provided by community pharmacists in Malaysia were inadequate compared to international practice principles. Areas that need improvement included collaboration with patients' other health care providers; more proactive management of patient's medicine regimen; having proper patient monitoring and follow-up mechanisms, and documentation.

    MeSH terms: Community Pharmacy Services*; Cross-Sectional Studies; Humans; Malaysia; Pharmacists*; Quality of Life; Surveys and Questionnaires; United States
  15. Sirajudeen AO, Law TH, Wong SV, Jakarni FM, Ng CP
    J Safety Res, 2021 09;78:262-269.
    PMID: 34399922 DOI: 10.1016/j.jsr.2021.06.007
    INTRODUCTION: Past empirical studies indicated that there is a Kuznets or reverse U-shaped relationship between road deaths and per capita income, such that the number of road death increases at a low level of per capita reverse U-shaped relationship was observed between road injuries and per capita income. While these studies explored the impact of per capita income on road deaths and road injuries, no studies have examined the relationship between per capita income and road death to road injury ratio (DPI).

    METHOD: Using a fixed effects panel regression analysis from 67 countries spanning over a period of five decades (1960-2013), the present study sought to explore the impact of per capita gross domestic product (per capita GDP) on the DPI ratio and the underlying factors responsible for the relationship.

    RESULTS: Our result suggests that per capita GDP followed a reverse U-shaped relationship with DPI. Moreover, the relative improvements in higher mobility roads as compared to improvements in higher accessibility roads, motorcycle ownership to passenger car ownership ratio, percentage of population living in an urban area, infant mortality rate, and the percentage of population below 15 years of age and above 64 years of age contributed to this relationship. Practical Applications: This implies that, at lower level of economic growth where road deaths exceed road injuries, countries should implement low-cost measures to combat road deaths cases. Such measures include mandating wearing of quality helmets and daytime running headlights for motorcycles. On the other hand, at higher level of economic growth where road injuries surpass road deaths, countries are encouraged to devote more resources to improving medical technology and services to treat road injury victims, mandating seatbelt use, as well as enhancing and promoting public transportation service.

    MeSH terms: Accidents, Traffic*; Humans; Infant; Motorcycles; Regression Analysis; Transportation; Economic Development*
  16. Chong ZX, Yeap SK, Ho WY
    Pharmacol Res, 2021 10;172:105818.
    PMID: 34400316 DOI: 10.1016/j.phrs.2021.105818
    Osteosarcoma is one of the most prevalent primary bone tumors with a high metastatic and recurrence rate with poor prognosis. MiRNAs are short and non-coding RNAs that could regulate various cellular activities and one of them is the epithelial-to-mesenchymal transition (EMT). Osteosarcoma cells that have undergone EMT would lose their cellular polarity and acquire invasive and metastatic characteristics. Our literature search showed that many pre-clinical and clinical studies have reported the roles of miRNAs in modulating the EMT process in osteosarcoma and compared to other cancers like breast cancer, there is a lack of review article which effectively summarizes the various roles of EMT-regulating miRNAs in osteosarcoma. This review, therefore, was aimed to discuss and summarize the EMT-promoting and EMT-suppressing roles of different miRNAs in osteosarcoma. The review would begin with the discussion on the concepts and principles of EMT, followed by the exploration of the diverse roles of EMT-regulating miRNAs in osteosarcoma. Subsequently, the potential use of miRNAs as prognostic biomarkers in osteosarcoma to predict the likelihood of metastases and as therapeutic agents would be discussed.
  17. Ponnuvelu K, Saniasiaya J, Abdul Gani N
    BMJ Case Rep, 2021 Aug 16;14(8).
    PMID: 34400422 DOI: 10.1136/bcr-2021-242122
    Foreign body (FB) in the external auditory canal is more common among the paediatric age group compared with adult patients and it may be deposited in various ways. An accidental animate aural FB is reported to be commonly encountered in adults whereas inanimate FBs are likely to be found among adult patients with learning disability. An elderly man presented with accidental penetration of rattan tree stem into his ear while gardening. Removal of a FB from the external auditory canal requires expertise as deep penetration of a FB into the middle and inner ear may lead grave complications, especially when overzealous removal is attempted by nonear, nose and throat (ENT) personnel. This case emphasises on the importance of awareness of meticulous removal of a FB under proper visualisation so as to avoid unnecessary complications. Additionally, we propose an algorithm for proper removal of aural FB in an acute care setting.
    MeSH terms: Adult; Aged; Algorithms; Child; Humans; Ear, Inner*; Male; Pharynx; Retrospective Studies
  18. Coppard SE, Jessop H, Lessios HA
    Sci Rep, 2021 Aug 16;11(1):16568.
    PMID: 34400682 DOI: 10.1038/s41598-021-95872-0
    The sea urchins Echinothrix calamaris and Echinothrix diadema have sympatric distributions throughout the Indo-Pacific. Diverse colour variation is reported in both species. To reconstruct the phylogeny of the genus and assess gene flow across the Indo-Pacific we sequenced mitochondrial 16S rDNA, ATPase-6, and ATPase-8, and nuclear 28S rDNA and the Calpain-7 intron. Our analyses revealed that E. diadema formed a single trans-Indo-Pacific clade, but E. calamaris contained three discrete clades. One clade was endemic to the Red Sea and the Gulf of Oman. A second clade occurred from Malaysia in the West to Moorea in the East. A third clade of E. calamaris was distributed across the entire Indo-Pacific biogeographic region. A fossil calibrated phylogeny revealed that the ancestor of E. diadema diverged from the ancestor of E. calamaris ~ 16.8 million years ago (Ma), and that the ancestor of the trans-Indo-Pacific clade and Red Sea and Gulf of Oman clade split from the western and central Pacific clade ~ 9.8 Ma. Time since divergence and genetic distances suggested species level differentiation among clades of E. calamaris. Colour variation was extensive in E. calamaris, but not clade or locality specific. There was little colour polymorphism in E. diadema.
    MeSH terms: Adenosine Triphosphatases/genetics; Animals; Calpain/genetics; Cell Nucleus/chemistry; DNA, Mitochondrial/genetics; DNA, Ribosomal/genetics; Biological Evolution; Gene Frequency; Introns/genetics; Pacific Ocean; Phylogeny; Pigmentation*; RNA, Ribosomal, 16S/genetics; RNA, Ribosomal, 28S/genetics; Sea Urchins/anatomy & histology; Sea Urchins/classification*; Sea Urchins/genetics; Species Specificity; Evolution, Molecular; Indian Ocean; Gene Flow*; Animal Distribution
  19. Makki M, Hassali MAA, Awaisu A, Chemaitelly H
    Saudi Pharm J, 2021 Jul;29(7):648-655.
    PMID: 34400858 DOI: 10.1016/j.jsps.2021.04.026
    Background: Unused medications in homes pose significant health, economic, and environmental risks. Patients are the medications end users and their knowledge, attitude, and practices (KAP) play an important role towards medication use, unuse and wastage. Thus, a valid instrument to reliably measure patients' KAP towards unused medications in homes may help manage the associated risks.

    Objective: To develop, translate, and validate a questionnaire for the assessment patients' KAP towards unused medications in Qatar homes (i.e., QUM-Qatar).

    Setting: This cross-sectional validation study was conducted among randomly selected outpatients visiting various public and private pharmacies in Qatar between September 2019 and February 2020.

    Method: Nine experts in the field of pharmacy practice with Qatar contextual background established the content validity of the instrument. The validity was quantified using content validity index (CVI). Furthermore, construct validity was performed using principal component analysis (PCA), whereas internal consistency reliability of items was determined using Cronbach's alpha. Statistical analyses were performed using STATA 15 statistical software.

    Main outcome measure: The psychometric properties of the QUM-Qatar assessment instrument.

    Results: An English/Arabic questionnaire was developed and validated. Content validity in the form of scale-level-CVI (S-CVI)/Average and S-CVI/UA was 0.88 and 0.84, respectively, suggesting adequate relevant content of the questionnaire. Variation explained by the multivariate model was 85.0% for knowledge, 94.8% for attitude, and 89.8% for practice. Cronbach's alpha coefficients were 0.68, 0.82, and 0.84, for knowledge, attitude, and practice domains, respectively. From the psychometric results obtained, the questionnaire's validity and reliability were attained.

    Conclusion: The QUM-Qatar instrument has acceptable psychometric properties and has the potential for future use in research and practice to assess KAP towards unused medications in Qatar and elsewhere. It may consequently help in improving medication use and mitigating the negative health, economic, and environmental impacts of unused medications.Impacts on practice.•There is now a valid and reliable English/Arabic language questionnaire to assess patients' KAP towards unused medications.•Policymakers can utilize this questionnaire to develop evidence-based policies and strategies for managing unused medications and their disposal.•To improve medication use review, rational use of medicines, and adherence, it is necessary to consider patient-reported outcomes that may eventually reflect on saving health, economic resources, and environment.

  20. Selvadurai S, Cheah KY, Ching MW, Kamaruddin H, Lee XY, Ngajidin RM, et al.
    Saudi Pharm J, 2021 Jul;29(7):670-676.
    PMID: 34400860 DOI: 10.1016/j.jsps.2021.04.028
    Background: Insulin injection technique re-education and diabetes knowledge empowerment has led to improved glycemic control.

    Objectives: To evaluate the impact of pharmacist's monthly re-education on insulin injection technique (IT), lipohypertrophy, patients' perception on insulin therapy and its effect on glycaemic control.

    Methods: This randomized controlled, multi-centered study was conducted among type 2 diabetics from 15 government health clinics. 160 diabetics with baseline HbA1C ≥ 8% and unsatisfactory IT technique were randomized into control or intervention group. Control group received standard pharmacist counselling during initiation and at 4th month. Intervention group received monthly counselling and IT re-education for 4 months. Assessment of diabetes, IT knowledge, adherence and perception towards diabetes were conducted using validated study tools Insulin Treatment Appraisal Scale (ITAS) and Medication Compliance Questionnaire (MCQ)).

    Results: 139 patients completed the study; control group (69), intervention group (70). In control group, all outcomes shown improvement except for patient's perception. Mean HbA1C decreased 0.79% ± 0.24 (p = 0.001). In intervention group, all outcomes improved significantly. HbA1c reduces significantly by 1.19% ± 0.10 (p 

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