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  1. Azadeh E, Abdullah UH, Ali NBM, Pizzi A, Gerardin-Charbonnier C, Gerardin P, et al.
    Polymers (Basel), 2022 Nov 19;14(22).
    PMID: 36433152 DOI: 10.3390/polym14225025
    Tannin-furanic foams were prepared with a good yield using the addition of relatively small proportions of a polyflavonoid tannin extract esterified with either palmitic acid, oleic acid, or lauric acid by its reaction with palmitoyl chloride, oleyl chloride, or lauryl chloride. FTIR analysis allowed us to ascertain the esterification of the tannin, and MALDI-TOF analysis allowed us to identify a number of multi-esterified flavonoid oligomers as well as some linked to residual carbohydrates related to the equally esterified tannin. These foams presented a markedly decreased surface friability or no friability at all, and at densities lower than the standard foam they were compared to. Equally, these experimental foams presented a much-improved water repellence, as indicated by their initial wetting angle, its small variation over time, and its stabilization at a high wetting angle value, while the wetting angle of the standard foam control went to zero very rapidly. This conclusion was supported by the calculation of the total surface energy of their surfaces as well as of their dispersive and polar components.
  2. Madadi R, Mohamadi S, Rastegari M, Karbassi A, Rakib MRJ, Khandaker MU, et al.
    Sci Rep, 2022 Nov 17;12(1):19736.
    PMID: 36396803 DOI: 10.1038/s41598-022-21242-z
    Rapid industrialization and urbanization have resulted in environmental pollution and unsustainable development of cities. The concentration of 12 potentially toxic metal(loid)s in windowsill dust samples (n = 50) were investigated from different functional areas of Qom city with the highest level of urbanization in Iran. Spatial analyses (ArcGIS 10.3) and multivariate statistics including Principal Component Analysis and Spearman correlation (using STATISTICA-V.12) were adopted to scrutinize the possible sources of pollution. The windowsill dust was very highly enriched with Sb (50 mg/kg) and Pb (1686 mg/kg). Modified degree of contamination (mCd) and the pollution load indices (PLIzone) indicate that windowsill dust in all functional areas was polluted in the order of industrial > commercial > residential > green space. Arsenic, Cd, Mo, Pb, Sb, Cu, and Zn were sourced from a mixture of traffic and industrial activities, while Mn in the dust mainly stemmed from mining activities. Non-carcinogenic health risk (HI) showed chronic exposure of Pb for children in the industrial zone (HI = 1.73). The estimations suggest the possible carcinogenic risk of As, Pb, and Cr in the dust. The findings of this study reveal poor environmental management of the city. Emergency plans should be developed to minimize the health risks of dust to residents.
  3. Razak MR, Aris AZ, Sukatis FF, Zaki MRM, Zainuddin AH, Haron DEM, et al.
    J Sep Sci, 2023 Jan;46(1):e2200282.
    PMID: 36337037 DOI: 10.1002/jssc.202200282
    In toxicological analysis, the analytical validation method is important to assess the exact risk of contaminants of emerging concern in the environment. Syringe filters are mainly used to remove impurities from sample solutions. However, the loss of analyte to the syringe filter could be considerable, causing an underestimate of the analyte concentrations. The current study develops and validates simultaneous liquid chromatography-mass spectrometry analysis using a direct filtration method to detect four groups of contaminants of emerging concern. The adsorption of the analyte onto three different matrices and six types of syringe filters is reported. The lowest adsorption of analytes was observed in methanol (16.72%), followed by deionized water (48.19%) and filtered surface lake water (48.94%). Irrespective of the type of the matrices, the lowest average adsorption by the syringe filter was observed in the 0.45 μm polypropylene membrane (15.15%), followed by the 0.20 μm polypropylene membrane (16.10%), the 0.20 μm regenerated cellulose (16.15%), the 0.20 μm polytetrafluoroethylene membrane (47.38%), the 0.45 μm nylon membrane (64.87%) and the 0.20 μm nylon membrane (71.30%). In conclusion, the recommended syringe filter membranes for contaminants of emerging concern analysis are polypropylene membranes and regenerated cellulose, regardless of the matrix used.
  4. Razak MR, Aris AZ, Zainuddin AH, Yusoff FM, Balia Yusof ZN, Kim SD, et al.
    Chemosphere, 2023 Feb;313:137377.
    PMID: 36457264 DOI: 10.1016/j.chemosphere.2022.137377
    Per- and polyfluoroalkyl substances (PFAS) are gaining worldwide attention because of their toxicity, bioaccumulative and resistance to biological degradation in the environment. PFAS can be categorised into endocrine disrupting chemicals (EDCs) and identified as possible carcinogenic agents for the aquatic ecosystem and humans. Despite this, only a few studies have been conducted on the aquatic toxicity of PFAS, particularly in invertebrate species such as zooplankton. This study evaluated the acute toxicity of two main PFAS, perfluorooctanoic acid (PFOA) and perfluorooctanesulfonate (PFOS), by using freshwater cladocerans (Moina micrura) as bioindicators. This study aimed to assess the adverse effects at different levels of organisations such as organ (heart size and heart rate), individual (individual size and mortality) and population (lethal concentration, LC50). PFOA was shown to be more hazardous than PFOS, with the LC50 values (confidence interval) of 474.7 (350.4-644.5) μg L-1 and 549.6 (407.2-743.9) μg L-1, respectively. As the concentrations of PFOS and PFOA increased, there were declines in individual size and heart rate as compared to the control group. The values of PNECs acquired by using the AF method (PNECAF) for PFOA and PFOS were 0.4747 and 0.5496 μg L-1, respectively. Meanwhile, the PNEC values obtained using the SSD method (PNECSSD) were 1077.0 μg L-1 (PFOA) and 172.5 μg L-1 (PFOS). PNECAF is more protective and conservative compared to PNECSSD. The findings of this study have significant implications for PFOS and PFOA risk assessment in aquatic environments. Thus, it will aid freshwater sustainability and safeguard the human dependency on water resources.
  5. Jamlos MA, Jamlos MF, Mustafa WA, Othman NA, Rohani MNKH, Saidi SA, et al.
    Nanomaterials (Basel), 2022 Dec 21;13(1).
    PMID: 36615938 DOI: 10.3390/nano13010027
    A low cost, with high performance, reduced graphene oxide (RGO) Ultra-wide Band (UWB) array sensor is presented to be applied with a technique of confocal radar-based microwave imaging to recognize a tumor in a human brain. RGO is used to form its patches on a Taconic substrate. The sensor functioned in a range of 1.2 to 10.8 GHz under UWB frequency. The sensor demonstrates high gain of 5.2 to 14.5 dB, with the small size of 90 mm × 45 mm2, which can be easily integrated into microwave imaging systems and allow the best functionality. Moreover, the novel UWB RGO array sensor is established as a detector with a phantom of the human head. The layers' structure represents liquid-imitating tissues that consist of skin, fat, skull, and brain. The sensor will scan nine different points to cover the whole one-sided head phantom to obtain equally distributed reflected signals under two different situations, namely the existence and absence of the tumor. In order to accurately detect the tumor by producing sharper and clearer microwave image, the Matrix Laboratory software is used to improve the microwave imaging algorithm (delay and sum) including summing the imaging algorithm and recording the scattering parameters. The existence of a tumor will produce images with an error that is lower than 2 cm.
  6. Loganathan TM, Sultan MTH, Ahsan Q, Jawaid M, Naveen J, Shah AUM, et al.
    J Therm Anal Calorim, 2022;147(24):14079-14096.
    PMID: 36093037 DOI: 10.1007/s10973-022-11557-4
    Natural fibers have emerged as a potential alternate to synthetic fibers, because of their excellent performance, biodegradability, renewability and sustainability. This research has focused on investigating the thermal, visco-elastic and fire-retardant properties of different hybrid Cytostachys Renda (CR)/kenaf fiber (K) (50/0; 35/ 15, 25/25, 15/ 35, 0/50)-reinforced MWCNT (multi-walled carbon nanotubes)-modified phenolic composites. The mass% of MWCNT-modified phenolic resin was maintained 50 mass% including 0.5 mass% of MWCNT. In order to achieve homogeneous dispersion ball milling process was employed to incorporate the MWCNT into phenolic resin (powder). Thermal results from thermogravimetric analysis and differential scanning calorimetric analysis revealed that the hybrid composites (35/15; 35 mass% CR and 15 mass% K) showed higher thermal stability among the composite samples. Visco-elastic results revealed that kenaf fiber-based MWCNT-modified composites (0/50; 0 mass% CR and 50 mass% K) exhibited higher storage and loss modulus due to high modulus kenaf fiber. Fire-retardant analysis (UL-94) showed that all the composite samples met H-B self-extinguishing rating and exhibited slow burning rate according to limiting oxygen index (LOI) test. However, (15/35; 15 mass% CR and 35 mass% K) hybrid composites showed the highest time to ignition, highest fire performance index, lowest total heat release rate, average mass loss rate, average fire growth rate index and maximum average rate of heat emission. Moreover, the smoke density of all hybrid composites was found to be less than 200 which meets the federal aviation regulations (FAR) 25.853d standard. Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) was carried out to select an optimal composite sample considering the thermal, visco-elastic and fire-retardant behaviors. Through TOPSIS analysis, the hybrid (15/35; 15 mass% CR and 35 mass% K) composite sample has been selected as an optimal composite which can be used for high-temperature aircraft and automotive applications.
  7. Mydin MAO, Abdullah MMAB, Mohd Nawi MN, Yahya Z, Sofri LA, Baltatu MS, et al.
    Materials (Basel), 2022 Dec 15;15(24).
    PMID: 36556790 DOI: 10.3390/ma15248984
    Foamed concrete is considered a green building material, which is porous in nature. As a result, it poses benefits such as being light in self-weight, and also has excellent thermal insulation properties, environmental safeguards, good fire resistance performance, and low cost. Nevertheless, foamed concrete has several disadvantages such as low strength, a large amount of entrained air, poor toughness, and being a brittle material, all of which has restricted its usage in engineering and building construction. Hence, this study intends to assess the potential utilization of polypropylene fibrillated fiber (PFF) in foamed concrete to enhance its engineering properties. A total of 10 mixes of 600 and 1200 kg/m3 densities were produced by the insertion of four varying percentages of PFF (1%, 2%, 3%, and 4%). The properties assessed were splitting tensile, compressive and flexural strengths, workability, porosity, water absorption, and density. Furthermore, the correlations between the properties considered were also evaluated. The outcomes reveal that the foamed concrete mix with 4% PFF attained the highest porosity, with approximately 13.9% and 15.9% for 600 and 1200 kg/m3 densities in comparison to the control specimen. Besides, the mechanical properties (splitting tensile, compressive and flexural strengths) increased steadily with the increase in the PFF percentages up to the optimum level of 3%. Beyond 3%, the strengths reduced significantly due to poor PFF dispersal in the matrix, leading to a balling effect which causes a degraded impact of scattering the stress from the foamed concrete vicinity to another area of the PFF surface. This exploratory investigation will result in a greater comprehension of the possible applications of PFF in LFC. It is crucial to promote the sustainable development and implementation of LFC materials and infrastructures.
  8. Jamil H, Din MFU, Tahir MJ, Saqlain M, Hassan Z, Khan MA, et al.
    PLoS Negl Trop Dis, 2022 Dec;16(12):e0010988.
    PMID: 36480553 DOI: 10.1371/journal.pntd.0010988
    BACKGROUND: Crimean-Congo hemorrhagic fever (CCHF) continues to pose a serious threat to the fragile healthcare system of Pakistan with a continuous increase of morbidity and mortality. The present study aimed to assess the knowledge, attitudes, and practices regarding CCHF among general people who resided in Pakistan.

    METHODS: An online cross-sectional survey design was applied, and a convenience sampling technique was used to recruit 1039 adult people from Pakistan. Data were collected from September 08 to October 12, 2021. The questionnaire consisted of a total of 32 questions in four parts assessing socio-demographics, as well as knowledge, attitudes, and practices regarding CCHF. All statistical analyses were performed using the Statistical Package for the Social Sciences (SPSS), and logistic regression analyses were performed to determine the factors associated with good knowledge, positive attitudes, and good practices.

    RESULTS: Alarmingly, 51.5% of participants heard about CCHF infection before administering the survey. Among these, 20.2%, 33.3%, and 48.2% of the study participants had demonstrated good knowledge, positive attitudes, and good practices, respectively. Binary logistic regression analysis revealed that education and income status had a significant impact on knowledge and attitudes (p<0.05). Similarly, the mean attitude scores differed significantly by age, education, and income status (p<0.05).

    CONCLUSIONS: The findings reflected inadequate levels of knowledge, attitudes, and practices regarding CCHF among general people in Pakistan which may regard as lower than expected. As CCHF is a highly contagious disease, it's urgent to initiate a comprehensive approach to handle the situation before it spreads further in Pakistan.

  9. Ahmad A, Jamil SNAM, Choong TSY, Abdullah AH, Faujan NH, Adeyi AA, et al.
    Polymers (Basel), 2022 Dec 10;14(24).
    PMID: 36559783 DOI: 10.3390/polym14245416
    Emerging dye pollution from textile industrial effluents is becoming more challenging for researchers worldwide. The contamination of water by dye effluents affects the living organisms in an ecosystem. Methylene blue (MB) and malachite green (MG) are soluble dyes with a high colour intensity even at low concentration and are hazardous to living organisms. The adsorption method is used in most wastewater plants for the removal of organic pollutants as it is cost-effective, has a high adsorption capacity, and good mechanical stabilities. In this study, a composite adsorbent was prepared by impregnating iron modified silica (FMS) onto polyurethane (PU) foam to produce an iron modified silica/polyurethane (FMS/PU) composite. The composite adsorbent was utilised in batch adsorption of the cationic dyes MB and MG. The effect of adsorption parameters such as the adsorbent load, pH, initial dye concentration, and contact time were discussed. Adsorption kinetics and isotherm were implemented to understand the adsorption mechanism for both dyes. It was found that the adsorption of MB and MG followed the pseudo-second order model. The Langmuir model showed a better fit than the Freundlich model for the adsorption of MB and MG, indicating that the adsorption occurred via the monolayer adsorption system. The maximum adsorption capacity of the FMS/PU obtained for MB was 31.7 mg/g, while for MG, it was 34.3 mg/g. The thermodynamic study revealed that the adsorption of MB and MG were exothermic and spontaneous at room temperature. In addition, the regeneration of FMS/PU was conducted to investigate the composite efficiency in adsorbing dyes for several cycles. The results showed that the FMS/PU composite could be regenerated up to four times when the regeneration efficiency dropped drastically to less than 20.0%. The impregnation of FMS onto PU foam also minimised the adsorbent loss into the environment.
  10. Mohamad Ismuddin S, Thambiah SC, Appannah G, Samsudin IN, Md Said S, Yeap SS, et al.
    Malays J Pathol, 2022 Dec;44(3):477-490.
    PMID: 36591715
    INTRODUCTION: Cardiovascular disease and osteoporosis (OP) have been shown to have similar risk factors but studies have demonstrated contradictory results with regards to their associations. This study evaluated relationships between bone characteristics and cardiovascular risk factors among adults in selected urban areas in Malaysia.

    MATERIALS AND METHODS: A cross-sectional study was performed involving 331 subjects between 45-90 years recruited at a health screening programme. Sociodemographic and clinical characteristics were recorded. Biochemical analyses on fasting blood samples and dual energy X-ray absorptiometry scan to determine bone mineral density (BMD) were performed.

    RESULTS: Increased waist circumference (WC) was protective for abnormal BMD status (osteopenia and OP). Males with increased high-density lipoprotein cholesterol (HDL) were more likely to be osteoporotic. WC, fasting blood glucose (FBG) and triglyceride (TG) were positively associated with BMD at all sites but was gender specific. In contrast, WC was negatively associated with trabecular bone score (TBS) for females but this association became attenuated when adjusted for fat percentage. HDL and MetS were negatively and positively associated with BMD, respectively in males.

    CONCLUSION: The cardiovascular risk factors of raised WC, FBG, TG and low HDL were significantly associated with increased BMD with skeletal site and gender specific differences after adjusting for confounders. However, a higher WC was associated with a weaker skeletal microstructure reflected by lower TBS in females driven by fat percentage. A higher BMD was demonstrated among MetS individuals. These findings suggest that adiposity may have a protective effect on BMD.

  11. Tan SC, Low TY, Hussain HMJ, Sharzehan MAK, Sito H, Kord-Varkaneh H, et al.
    PLoS One, 2022;17(10):e0276313.
    PMID: 36264998 DOI: 10.1371/journal.pone.0276313
    BACKGROUND: The XRCC3 p.Thr241Met (rs861539) polymorphism has been extensively studied for its association with glioma risk, but results remain conflicting. Therefore, we performed a systematic review and meta-analysis to resolve this inconsistency.

    METHODS: Studies published up to June 10, 2022, were searched in PubMed, Web of Science, Scopus, VIP, Wanfang, and China National Knowledge Infrastructure databases and screened for eligibility. Then, the combined odds ratio (OR) of the included studies was estimated based on five genetic models, i.e., homozygous (Met/Met vs. Thr/Thr), heterozygous (Thr/Met vs. Thr/Thr), dominant (Thr/Met + Met/Met vs. Thr/Thr), recessive (Met/Met vs. Thr/Thr + Thr/Met) and allele (Met vs. Thr). The study protocol was preregistered at PROSPERO (registration number: CRD42021235704).

    RESULTS: Overall, our meta-analysis of 14 eligible studies involving 12,905 subjects showed that the p.Thr241Met polymorphism was significantly associated with increased glioma risk in both homozygous and recessive models (homozygous, OR = 1.381, 95% CI = 1.081-1.764, P = 0.010; recessive, OR = 1.305, 95% CI = 1.140-1.493, P<0.001). Subgroup analyses by ethnicity also revealed a statistically significant association under the two aforementioned genetic models, but only in the Asian population and not in Caucasians (P>0.05).

    CONCLUSION: We demonstrated that the XRCC3 p.Thr241Met polymorphism is associated with an increased risk of glioma only in the homozygous and recessive models.

  12. Hafizh M, Soliman MM, Qiblawey Y, Chowdhury MEH, Islam MT, Musharavati F, et al.
    Biosensors (Basel), 2023 Jan 02;13(1).
    PMID: 36671914 DOI: 10.3390/bios13010079
    In this paper, a surface acoustic wave (SAW) sensor for hip implant geometry was proposed for the application of total hip replacement. A two-port SAW device was numerically investigated for implementation with an operating frequency of 872 MHz that can be used in more common radio frequency interrogator units. A finite element analysis of the device was developed for a lithium niobate (LiNBO3) substrate with a Rayleigh velocity of 3488 m/s on COMSOL Multiphysics. The Multiphysics loading and frequency results highlighted a good uniformity with numerical results. Afterwards, a hip implant geometry was developed. The SAW sensor was mounted at two locations on the implant corresponding to two regions along the shaft of the femur bone. Three discrete conditions were studied for the feasibility of the implant with upper- and lower-body loading. The loading simulations highlighted that the stresses experienced do not exceed the yield strengths. The voltage output results indicated that the SAW sensor can be implanted in the hip implant for hip implant-loosening detection applications.
  13. Milad A, Babalghaith AM, Al-Sabaeei AM, Dulaimi A, Ali A, Reddy SS, et al.
    Int J Environ Res Public Health, 2022 Nov 11;19(22).
    PMID: 36429580 DOI: 10.3390/ijerph192214863
    The environmental concerns of global warming and energy consumption are among the most severe issues and challenges facing human beings worldwide. Due to the relatively higher predicted temperatures (150-180 °C), the latest research on pavement energy consumption and carbon dioxide (CO2) emission assessment mentioned contributing to higher environmental burdens such as air pollution and global warming. However, warm-mix asphalt (WMA) was introduced by pavement researchers and the road construction industry instead of hot-mix asphalt (HMA) to reduce these environmental problems. This study aims to provide a comparative overview of WMA and HMA from environmental and economic perspectives in order to highlight the challenges, motivations, and research gaps in using WMA technology compared to HMA. It was discovered that the lower production temperature of WMA could significantly reduce the emissions of gases and fumes and thus reduce global warming. The lower production temperature also provides a healthy work environment and reduces exposure to fumes. Replacing HMA with WMA can reduce production costs because of the 20-75% lower energy consumption in WMA production. It was also released that the reduction in energy consumption is dependent on the fuel type, energy source, material heat capacity, moisture content, and production temperature. Other benefits of using WMA are enhanced asphalt mixture workability and compaction because the additives in WMA reduce asphalt binder viscosity. It also allows for the incorporation of more waste materials, such as reclaimed asphalt pavement (RAP). However, future studies are recommended on the possibility of using renewable, environmentally friendly, and cost-effective materials such as biomaterials as an alternative to conventional WMA-additives for more sustainable and green asphalt pavements.
  14. Pervez MN, Yeo WS, Shafiq F, Jilani MM, Sarwar Z, Riza M, et al.
    Heliyon, 2023 Jan;9(1):e12883.
    PMID: 36691543 DOI: 10.1016/j.heliyon.2023.e12883
    Given the carcinogenic properties of formaldehyde-based chemicals, an alternative method for resin-finishing cotton textiles is urgently needed. Therefore, the primary objective of this study is to introduce a sustainable resin-finishing process for cotton fabric via an industrial procedure. For this purpose, Bluesign® approved a formaldehyde-free Knittex RCT® resin was used, and the process parameters were designed and optimized according to the Taguchi L27 method. XRD analysis confirmed the crosslinking formation between resin and neighboring molecules of cotton fabric, as no change in the cellulose crystallization phase. Several machine learning models were built in a sequence to predict the crease recovery angle (CRA), tearing strength (TE) and whiteness index (WI). Assessment of modelling was evaluated through the use of various metrics such as root mean square error (RMSE), mean absolute error (MAE), and the coefficient of determination (R2). Results were compared to those from other regression models, such as principal component regression (PCR), partial least squares regression (PLSR), and fuzzy modelling. Based on the results of our research, the LSSVR model predicted the CRA, TE, and WI with substantially more accuracy than other models, as shown by the fact that its RMSE and MAE values were significantly lower. In addition, it offered the greatest possible R2 values, reaching up to 0.9627.
  15. Ibitoye MO, Hamzaid NA, Hasnan N, Abdul Wahab AK, Islam MA, Kean VS, et al.
    Med Eng Phys, 2016 Aug;38(8):767-75.
    PMID: 27289541 DOI: 10.1016/j.medengphy.2016.05.012
    The interaction between muscle contractions and joint loading produces torques necessary for movements during activities of daily living. However, during neuromuscular electrical stimulation (NMES)-evoked contractions in persons with spinal cord injury (SCI), a simple and reliable proxy of torque at the muscle level has been minimally investigated. Thus, the purpose of this study was to investigate the relationships between muscle mechanomyographic (MMG) characteristics and NMES-evoked isometric quadriceps torques in persons with motor complete SCI. Six SCI participants with lesion levels below C4 [(mean (SD) age, 39.2 (7.9) year; stature, 1.71 (0.05) m; and body mass, 69.3 (12.9) kg)] performed randomly ordered NMES-evoked isometric leg muscle contractions at 30°, 60° and 90° knee flexion angles on an isokinetic dynamometer. MMG signals were detected by an accelerometer-based vibromyographic sensor placed over the belly of rectus femoris muscle. The relationship between MMG root mean square (MMG-RMS) and NMES-evoked torque revealed a very high association (R(2)=0.91 at 30°; R(2)=0.98 at 60°; and R(2)=0.97 at 90° knee angles; P<0.001). MMG peak-to-peak (MMG-PTP) and stimulation intensity were less well related (R(2)=0.63 at 30°; R(2)=0.67 at 60°; and R(2)=0.45 at 90° knee angles), although were still significantly associated (P≤0.006). Test-retest interclass correlation coefficients (ICC) for the dependent variables ranged from 0.82 to 0.97 for NMES-evoked torque, between 0.65 and 0.79 for MMG-RMS, and from 0.67 to 0.73 for MMG-PTP. Their standard error of measurements (SEM) ranged between 10.1% and 31.6% (of mean values) for torque, MMG-RMS and MMG-PTP. The MMG peak frequency (MMG-PF) of 30Hz approximated the stimulation frequency, indicating NMES-evoked motor unit firing rate. The results demonstrated knee angle differences in the MMG-RMS versus NMES-isometric torque relationship, but a similar torque related pattern for MMG-PF. These findings suggested that MMG was well associated with torque production, reliably tracking the motor unit recruitment pattern during NMES-evoked muscle contractions. The strong positive relationship between MMG signal and NMES-evoked torque production suggested that the MMG might be deployed as a direct proxy for muscle torque or fatigue measurement during leg exercise and functional movements in the SCI population.
  16. Nor FHM, Abdullah S, Ibrahim Z, Nor MHM, Osman MI, Al Farraj DA, et al.
    Bioprocess Biosyst Eng, 2023 Mar;46(3):381-391.
    PMID: 35779113 DOI: 10.1007/s00449-022-02749-1
    An effective biosurfactant producer and extremophiles bacteria, Bacillus cereus KH1, was isolated from textile effluent and the biosurfactant was produced using molasses as the sole carbon source. Growth parameters such as pH, temperature, salinity and concentration of molasses were optimised for decolourising the textile effluent with 24-h incubation. The biosurfactant property of B. cereus KH1 was evaluated based on haemolytic activity, oil displacement technique, drop-collapsing test and emulsification index. The results of the produced biosurfactant showed a positive reaction in haemolytic activity, oil displacement technique, drop-collapsing test and exhibiting a 67% emulsification index. The cell-free broth was stable in 40 °C pH 7, 7% salinity and 7% molasses. Thin-Layer Chromatography and Fourier Transform Infrared Spectroscopy analysis revealed that the biosurfactant was a lipopeptide with a yield 2.98 g L-1. These findings proved the synergistic action of B. cereus KH1 with lipopeptide biosurfactant may accelerated the decolourisation efficiency to 87%.
  17. Tasrip NA, Mohd Desa MN, Khairil Mokhtar NF, Sajali N, Mohd Hashim A, Ali ME, et al.
    J Food Sci Technol, 2021 Dec;58(12):4504-4513.
    PMID: 34629514 DOI: 10.1007/s13197-020-04932-2
    Low DNA concentration recovered from highly processed products such as gelatin and gelatin-based products renders difficulty in detecting porcine contamination using conventional PCR techniques. We documented here a porcine-specific loop-mediated isothermal amplification (LAMP) to identify porcine traces in gelatin products. The porcine-specific primers were designed according to mitochondrial DNA of Cytochrome b gene sequence. Here we used two different reaction mixtures for LAMP assay (GENIE and MYRM) against the same DNA samples extracted from gelatin products and porcine-specific primers to detect the presence of porcine DNA. The porcine-specific primers were shown to be specific only to Sus scrofa against 14 DNA of other meat species. The analytical sensitivity of the LAMP assay for porcine DNA detection is 1 pg/µL using both GENIE (within 30 m) and MYRM (within 60 m) reaction mixtures. Analysis against 32 samples of gelatin products showed that five samples were found to contain porcine DNA; two samples out of six gelatin powder samples and three gelatin capsule samples out of nine. Out of these five positive samples, three were not labeled containing porcine gelatin. Overall, LAMP assay in this study showed an excellent specificity, sensitivity and rapidity in detection of porcine DNA in gelatin products.

    SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at (10.1007/s13197-020-04932-2).

  18. Lednicky JA, Tagliamonte MS, White SK, Blohm GM, Alam MM, Iovine NM, et al.
    Clin Infect Dis, 2022 Aug 24;75(1):e1184-e1187.
    PMID: 34718467 DOI: 10.1093/cid/ciab924
    We isolated a novel coronavirus from a medical team member presenting with fever and malaise after travel to Haiti. The virus showed 99.4% similarity with a recombinant canine coronavirus recently identified in a pneumonia patient in Malaysia, suggesting that infection with this virus and/or recombinant variants occurs in multiple locations.
  19. Butt MD, Ong SC, Wahab MU, Rasool MF, Saleem F, Hashmi A, et al.
    Int J Environ Res Public Health, 2022 Oct 02;19(19).
    PMID: 36231911 DOI: 10.3390/ijerph191912611
    BACKGROUND: Diabetes is a major chronic illness that negatively influences individuals and society. Therefore, this research aimed to analyze and evaluate the cost associated with diabetes management, specific to the Pakistani Type 2 diabetes population. Research scheme and methods: A survey randomly collected information and data from diabetes patients throughout Pakistan out-patient clinics. Direct and indirect costs were evaluated, and data were analyzed with descriptive and inferential statistics.

    RESULTS: An overall of 1839 diabetes patients participated in the study. The results have shown that direct and indirect costs are positively associated with the participants' socio-demographic characteristics, except for household income and educational status. The annual total cost of diabetes care was USD 740.1, amongst which the share of the direct cost was USD 646.7, and the indirect cost was USD 93.65. Most direct costs comprised medicine (USD 274.5) and hospitalization (USD 319.7). In contrast, the productivity loss of the patients had the highest contribution to the indirect cost (USD 81.36).

    CONCLUSION: This study showed that direct costs significantly contributed to diabetes's overall cost in Pakistan and overall diabetes management estimated to be 1.67% (USD 24.42 billion) of the country's total gross domestic product. The expense of medications and hospitalization mostly drove the direct cost. Additionally, patients' loss of productivity contributed significantly to the indirect cost. It is high time for healthcare policymakers to address this huge healthcare burden. It is time to develop a thorough diabetes management plan to be implemented nationwide.

  20. Said MA, Sulaiman AH, Habil MH, Das S, Bakar AK, Yusoff RM, et al.
    Singapore Med J, 2012 Dec;53(12):801-7.
    PMID: 23268153
    INTRODUCTION:This study aimed to determine the prevalence of metabolic syndrome and risk of coronary heart disease (CHD) in patients with schizophrenia receiving antipsychotics in Malaysia.
    METHODS:This cross-sectional study, conducted at multiple centres, involved 270 patients who fulfilled the Diagnostic and Statistical Manual of Mental Disorders (DSM)-IV-TR diagnostic criteria for schizophrenia, were on antipsychotic medications for at least one year, and were screened for metabolic syndrome. Patients receiving mood stabilisers were excluded. Metabolic syndrome was defined according to the National Cholesterol Education Program ATP III criteria modified for Asian waist circumference. Risk for cardiovascular disease was assessed by using Framingham function (all ten-year CHD events).
    RESULTS:The prevalence of metabolic syndrome was 46.7% (126/270). Among all the antipsychotics used, atypical antipsychotics (monotherapy) were most commonly used in both the metabolic and non-metabolic syndrome groups (50.8% vs. 58.3%). The ten-year risk for CHD was significantly higher in patients with metabolic syndrome. The proportion of patients with high/very high risk for CHD (Framingham ≥ 10%) was greater in patients with metabolic syndrome than in those with non-metabolic syndrome (31.5% vs. 11.0%, odds ratio 3.9, 95% confidence interval 2.0-7.6; p < 0.001). The mean body mass index was higher in patients with metabolic syndrome than in those without (29.4 ± 5.1 kg/m2 vs. 25.0 ± 5.6 kg/m2; p < 0.001).
    CONCLUSION:Patients with schizophrenia receiving antipsychotics in Malaysia have a very high incidence of metabolic syndrome and increased cardiovascular risk. Urgent interventions are needed to combat these problems in patients.
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