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Characterization of edible bird's nest of different production, species and geographical origins using nutritional composition, physicochemical properties and antioxidant activities

Quek MC, Chin NL, Yusof YA, Law CL, Tan SW

Food Res Int, 2018 07;109:35-43.
PMID: 29803459 DOI: 10.1016/j.foodres.2018.03.078

Edible bird's nest (EBN) is a precious food made from the solidified saliva of swiftlets. EBN from three types of origin, namely production, swiftlet species and geographical were characterised based on its nutritional composition, physicochemical properties and antioxidant properties. Proximate composition, total phenolic content (TPC) and antioxidant activities were determined following official methods, while mineral and heavy metal contents were obtained by respective atomic adsorption spectrometry (AAS) and inductively coupled plasma-mass spectrometry (ICP-MS). Amino acids profile and sialic acid were determined using high performance liquid chromatography (HPLC). Calcium and sodium were the major elements in EBN samples at averages of 17,267 mg/kg and 13,681 mg/kg, respectively. Despite protein contents were not significantly different; interestingly the total amino acids in A. fuciphagus EBN, 64.57 g/100 g was found to be 23% higher than in A. maximus EBN. EBN from house, A. fuciphagus and Peninsular Malaysia had greater antioxidant activities, 2.33-3.49 mg AAE/g and higher sialic acid, 13.57 g/100 g while those from cave, A. maximus and East Malaysia contained more minerals like calcium and magnesium. The 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and ferric ion reducing antioxidant power (FRAP) of house, A. fuciphagus and Peninsular Malaysia EBNs were approximately 2 times greater than the others. All samples were complied with the Malaysian Standard MS 2334:2011, except for mercury and nitrite. The overall findings suggest that the quality of EBN was varied following the production, species and geographical origins.

Matched MeSH terms: Chromatography, High Pressure Liquid
Fulltext Design and fabrication of brake pad testing apparatus for teaching and learning application

Leman, A.M., Che Wan Izzudin, Md Zin Ibrahim, Dafit Feriyanto

Journal of Occupational Safety and Health, 2015;12(2):87-92.
MyJurnal

Brake pad apparatus is designed for help student and instructor in teaching and learning application. The objective
of this research is to differentiate the pressure effect and braking temperature condition of different pad. This apparatus
also aimed for learning the safety car and motorcycle braking system. This apparatus can to compare with theoretical
calculation in order to approve that this apparatus is useful. The main concept in this apparatus is thermocouple use
to detect the temperature gain while braking process. Speed motor controller used for set the angular velocity of the
motor in braking process. Pressure applied at brake pedal detected by pressure gauge and data logger function as a
connector. This apparatus also designed based on valid data for average of teenager in Malaysia which made on a
sample university student. Result show that the apparatus can function effectively by defines the different temperature
when applied the different pressure and different pad. Pad C shows the 880C for thermocouple 1 and 790C for
thermocouple 2 at the 20 psi and infrared thermometer show 1130C for pad C. Graph from calculation shows that the
pad A have 216.480C at 1000 rpm which have low temperature than pad B, C and D. high efficiency of friction and
pressure applied will cause more heat generate than low coefficient of friction and pressure applied.

Matched MeSH terms: Pressure
Fulltext Mandibular advancement by distraction Osteogenesis for obstructive sleep apnea in patient with Pierre Robin Sequence: a case report

Mohamed Hisham Mohamed Jali @ Yunos, Shaifulizan Abdul Rahman, Ramizu Shaari

International Medical Journal Malaysia, 2017;16(21):28-28.
MyJurnal

Pierre Robin Sequence (PRS) is a condition consists of set of anomalies, which are
cleft palate, micrognathia and glossoptopsis. Management of patients with PRS addresses two main
problems, namely airway obstruction and feeding difficulties. Airway obstruction may lead to
obstructive sleep apnea (OSA). Treatment modalities for OSA are based on the causes. There are
surgical and non-surgical methods. Non-surgical methods such as diet, medication, oral appliances
and continuous positive airway pressure (CPAP) can only be employed in moderate cases. Surgical
method such as maxillo-mandibular advancement or expansion can be achieved by orthognathic
surgery or distraction osteogenesis. (Copied from article).

Matched MeSH terms: Continuous Positive Airway Pressure
Production of palm kernel shell-based activated carbon by direct physical activation for carbon dioxide adsorption

Rashidi NA, Yusup S

Environ Sci Pollut Res Int, 2019 Nov;26(33):33732-33746.
PMID: 29740771 DOI: 10.1007/s11356-018-1903-8

The feasibility of biomass-based activated carbons has received a huge attention due to their excellent characteristics such as inexpensiveness, good adsorption behaviour and potential to reduce a strong dependency towards non-renewable precursors. Therefore, in this research work, eco-friendly activated carbon from palm kernel shell that has been produced from one-stage physical activation by using the Box-Behnken design of Response Surface Methodology is highlighted. The effect of three input parameters-temperature, dwell time and gas flow rate-towards product yield and carbon dioxide (CO2) uptake at room temperature and atmospheric pressure are studied. Model accuracy has been evaluated through the ANOVA analysis and lack-of-fit test. Accordingly, the optimum condition in synthesising the activated carbon with adequate CO2 adsorption capacity of 2.13 mmol/g and product yield of 25.15 wt% is found at a temperature of 850 °C, holding time of 60 min and CO2 flow rate of 450 cm3/min. The synthesised activated carbon has been characterised by diverse analytical instruments including thermogravimetric analyser, scanning electron microscope, as well as N2 adsorption-desorption isotherm. The characterisation analysis indicates that the synthesised activated carbon has higher textural characteristics and porosity, together with better thermal stability and carbon content as compared to pristine palm kernel shell. Activated carbon production via one-step activation approach is economical since its carbon yield is within the industrial target, whereas CO2 uptake is comparable to the synthesised activated carbon from conventional dual-stage activation, commercial activated carbon and other published data from literature.

Matched MeSH terms: Atmospheric Pressure
Fulltext Diagnostic dilemma of dural carotid cavernous fistula

Noorlaila B., Zunaina E., Raja Norliza R.O., Nor Fadzillah A.J., Alice, G.K.C.

Journal of Biomedical and Clinical Sciences, 2017;2(1):38-41.
MyJurnal

We report a case of dural carotid cavernous fistula (CCF) pose a diagnostic dilemma with initial symptoms of the arteriovenous shunt. A 56 year-old man presented with right eye diplopia, thensubsequently developed ptosis, congestion of conjunctiva, dilated episcleral vessels, and gradual rise in intraocular pressure. Initial diagnosis of pseudotumour was made based on negative finding of CCF by computed tomography angiography (CTA). In view of persistent clinical manifestations in spite of steroid therapy, and with the presence of new ocular signs; cock-screw conjunctival vessels, dilated retinal veins, and proptosis, digital subtraction angiography (DSA) was performed and confirmed the diagnosis of dural CCF.The ocular symptoms resolved completely post embolization of the fistula.

Matched MeSH terms: Intraocular Pressure
Selection of inactivation medium for fungal spores in clinical wastes by supercritical carbon dioxide

Noman E, Norulaini Nik Ab Rahman N, Al-Gheethi A, Nagao H, Talip BA, Ab Kadir O

Environ Sci Pollut Res Int, 2018 Aug;25(22):21682-21692.
PMID: 29785605 DOI: 10.1007/s11356-018-2335-1

The present study aimed to select the best medium for inactivation of Aspergillus fumigatus, Aspergillus spp. in section Nigri, A. niger, A. terreus var. terreus, A. tubingensis, Penicillium waksmanii, P. simplicissimum, and Aspergillus sp. strain no. 145 spores in clinical wastes by using supercritical carbon dioxide (SC-CO2). There were three types of solutions used including normal saline, seawater, distilled water, and physiological saline with 1% of methanol; each solution was tested at 5, 10, and 20 mL of the water contents. The experiments were conducted at the optimum operating parameters of supercritical carbon dioxide (30 MPa, 75 °C, 90 min). The results showed that the inactivation rate was more effective in distilled water with the presence of 1% methanol (6 log reductions). Meanwhile, the seawater decreases inactivation rate more than normal saline (4.5 vs. 5.1 log reduction). On the other hand, the experiments performed with different volumes of distilled water (5, 10, and 20 mL) indicated that A. niger spores were completely inactivated with 10 mL of distilled water. The inactivation rate of fungal spores decreased from 6 to 4.5 log as the amount of distilled water increased from 10 to 20 mL. The analysis for the spore morphology of A. fumigatus and Aspergillus spp. in section Nigri using scanning electron microscopy (SEM) has revealed the role of temperature and pressure in the SC-CO2 in the destruction of the cell walls of the spores. It can be concluded that the distilled water represent the best medium for inactivation of fungal spores in the clinical solid wastes by SC-CO2.

Matched MeSH terms: Pressure
Fulltext Novel method for gelatin extraction of various local fish using High Pressure Processing (HPP)

Jamal, P., Jaswir, I., Yusof, N., Jami, M.S.

International Food Research Journal, 2017;24(101):533-539.
MyJurnal

Gelatin from fish skin is known to be an alternative source for mammalian gelatin. However, it has weaker properties compared to bovine and porcine gelatin, which limits its use in the industry. The conventional method for fish gelatin extraction requires long production time and could cause serious water pollution and chemical treatments are often being used to enhance the yield of fish gelatin and its properties but it may affect the amino acid content of the gelatin. In this regard, High-Pressure Processing (HPP) is a novel method suggested for fish gelatin extraction. The HPP method is classified as green technology as it requires low electricity throughout the process. This study will discuss the impact of HPP the technique gelatin extracted from fish skin. Skins from four types of fish, namely red tilapia (Oreochromis niloticus), black tilapia (Oreochromis mossambicus), grouper (Epinephelus areolatus) and threadfin bream (Nemipterus tambuloides), were used. High pressure was applied at either pretreatment in citric acid solution or during thermal extraction; and the pressure was maintained at 250 MPa with pressure holding time of 10 minutes and 18 hours of water extraction. Gelatin extract from traditional acid-base method was prepared as a standard for comparison. The study found that there was an increment in the yield of gelatin and the concentration of gelatin extract, and the pre-treatment time was also reduced.

Matched MeSH terms: Pressure
Fulltext Choroidal melanoma – a silent killer

Pei, Fang Neoh, Tai, Evelyn L.M., Liza-Sharmini A.T.

Journal of Biomedical and Clinical Sciences, 2017;2(2):29-31.
MyJurnal

A 65-year-old lady complained of occasional flashes of light over her left eye for 2 months. She was referred for ocular assessment after she underwent refractive assessment at the optometrist. On examination, best corrected visual acuity in both eyes was 6/9. Both eyes anterior segments were normal, with normal intraocular pressure. The pupils were equal with no relative afferent pupillary defect. Left eye fundus examination was normal. On the contrary, the "normal" right eye fundus examination revealed a huge, well-circumscribed hypo-pigmented elevated choroidal mass. B scan ultrasonography of the right eye showed a mushroom-shaped intraocular mass. Magnetic resonance imaging showed an intraocular lesion. Comprehensive systemic examination and investigations to rule out distant primary malignancy were unremarkable. A provisional diagnosis of right eye primary choroidal melanoma was made. Despite good vision in the affected eye, she underwent right eye enucleation in view of the large intraocular mass. The diagnosis was confirmed by histopathological examination.

Matched MeSH terms: Intraocular Pressure
Fulltext Herpes Zoster Ophthalmicus, persistent hyphema and orbital apex syndrome; a blinding combination

Shavani, Abirami, Adil Hussein, Wan-Hazabbah W.H.

Journal of Biomedical and Clinical Sciences, 2017;2(2):48-51.
MyJurnal

To report a rare case of an elderly gentleman who presented with herpes zoster ophthalmicus, complicated with persistent hyphema and orbital apex syndrome. A 75-year-old Malay gentleman presented with left herpes zoster ophthalmicus that was complicated with complete ophthalmoplegia and ptosis. He developed total hyphema in the affected eye with a secondary elevated intraocular pressure after a week. He was treated with oral acyclovir and topical corticosteroids. However, the total hyphema persisted that required an anterior chamber washout surgery. Herpes Zoster Ophthalmicus complicated with persistent hyphema and orbital apex syndrome is rare and very challenging to manage. Radiological imaging is important to exclude other causes of OAS. It is recommended to treat HZO with systemic acyclovir for a longer duration in view of ocular and neurological involvement.

Matched MeSH terms: Intraocular Pressure
Fulltext Pulsatile operation of a continuous-flow right ventricular assist device (RVAD) to improve vascular pulsatility

Ng BC, Kleinheyer M, Smith PA, Timms D, Cohn WE, Lim E

PLoS One, 2018;13(4):e0195975.
PMID: 29677212 DOI: 10.1371/journal.pone.0195975

Despite the widespread acceptance of rotary blood pump (RBP) in clinical use over the past decades, the diminished flow pulsatility generated by a fixed speed RBP has been regarded as a potential factor that may lead to adverse events such as vasculature stiffening and hemorrhagic strokes. In this study, we investigate the feasibility of generating physiological pulse pressure in the pulmonary circulation by modulating the speed of a right ventricular assist device (RVAD) in a mock circulation loop. A rectangular pulse profile with predetermined pulse width has been implemented as the pump speed pattern with two different phase shifts (0% and 50%) with respect to the ventricular contraction. In addition, the performance of the speed modulation strategy has been assessed under different cardiovascular states, including variation in ventricular contractility and pulmonary arterial compliance. Our results indicated that the proposed pulse profile with optimised parameters (Apulse = 10000 rpm and ωmin = 3000 rpm) was able to generate pulmonary arterial pulse pressure within the physiological range (9-15 mmHg) while avoiding undesirable pump backflow under both co- and counter-pulsation modes. As compared to co-pulsation, stroke work was reduced by over 44% under counter-pulsation, suggesting that mechanical workload of the right ventricle can be efficiently mitigated through counter-pulsing the pump speed. Furthermore, our results showed that improved ventricular contractility could potentially lead to higher risk of ventricular suction and pump backflow, while stiffening of the pulmonary artery resulted in increased pulse pressure. In conclusion, the proposed speed modulation strategy produces pulsatile hemodynamics, which is more physiologic than continuous blood flow. The findings also provide valuable insight into the interaction between RVAD speed modulation and the pulmonary circulation under various cardiovascular states.

Matched MeSH terms: Blood Pressure
Fulltext Dislocated posterior chamber intraocular lens (PCOIL) in patients with retinitis pigmentosa (RP)

Lam, C.S., Mushawiahti, M., Bastion, M.L.C.

Journal of Surgical Academia, 2017;7(1):38-42.
MyJurnal

Subluxation or dislocation of PCIOL is one of the complications of cataract operation in RP patients. This paper reports the presentation of PCIOL dislocation and subluxation and the management and outcome in 3 eyes of 2 RP patients. Two medical records of patients with RP who developed dislocated or subluxated PCIOL and subsequently underwent explantation of the dropped IOL were evaluated. Two patients had bilateral eye cataract operation done and had PCIOL implanted. Patient 1 developed left eye subluxated PCIOL inferiorly after 2 years of the cataract operation and right eye dislocated PCIOL anteriorly 4 years after cataract operation. Patient 2 develop right eye subluxated PCIOL inferiorly after 12 years of the cataract operation. Patient 1 with right eye dislocated PCIOL underwent intraocular lens (IOL) explantation and was left aphakic as her visual prognosis was poor due to advanced RP. The left IOL remained within the visual axis despite subluxation and no intervention has been done. Patient 2 with right eye subluxated PCIOL underwent IOL explantation and anterior chamber intraocular lens (ACIOL) implantation. ACIOL remained stable and visual acuity improved post-operation. Both the operations were uneventful. Post-operatively, there was no elevated intraocular pressure and no prolonged ocular inflammation, which required prolonged anti-inflammatory and no retinal detachment was seen. Both patient and surgeon should be aware of potential PCIOL subluxation or dislocation in RP. The presentation may be as late as more than a decade after the cataract operation.

Matched MeSH terms: Intraocular Pressure
Fulltext Effect of various food processing and handling methods on preservation of natural antioxidants in fruits and vegetables

Al-Juhaimi F, Ghafoor K, Özcan MM, Jahurul MHA, Babiker EE, Jinap S, et al.

J Food Sci Technol, 2018 Oct;55(10):3872-3880.
PMID: 30228385 DOI: 10.1007/s13197-018-3370-0

Bioactive compounds from plant sources are generally categorized as natural antioxidants with well-known health benefits. The health-promoting characteristics of natural antioxidants include anti-inflammatory, anti-diabetic, and hepatic effects as well as free radical scavenging. Herein, a comprehensive and comparative review are presented about the effects of conventional (thermal and mechanical) and relatively new (non-thermal) processing methods on phytochemicals and discussed the importance of implementing the use of those methods that could be of very helpful retaining the quality of the bioactive compounds in plant-based foods. Plant-based foods rich in phenolics, vitamin C, carotenoids, and other compounds undergo a range of processing operations before they are consumed. Most of these methods involve thermal treatments of fruits, stems, leaves, and roots. These techniques have varying effects on bioactive compounds and their activities, and the magnitude of these effects depends on process parameters such as temperature, time, and the food matrix. Thermal processing can be detrimental to bioactive compounds while nonthermal procedures may not cause significant deterioration of important health-promoting phytochemicals and in some cases can improve their bio-activity and bio-availability. The detrimental effects of conventional processing on the quality of natural antioxidants have been compared to the effects of innovative nonthermal food treatments such as gamma and ultraviolet irradiation, ultraviolet light, pulsed electric fields, and high hydrostatic pressure.

Matched MeSH terms: Hydrostatic Pressure
Endocan expression in placenta of women with hypertension

Chew BS, Ghazali R, Othman H, Ismail NAM, Othman AS, Laim NMST, et al.

J Obstet Gynaecol Res, 2018 Oct 10.
PMID: 30306675 DOI: 10.1111/jog.13836

AIM: The aim of our study was to determine the endocan-1 expression in placenta of hypertensive women, and its association with maternal and fetal outcomes.
METHODS: This was a cross-sectional study consisted of 21 pregnant women with hypertension and 23 without hypertension. The gestational age ranged from 28 to 39 weeks (hypertensive) and 32 to 40 weeks (normotensive). The paraffin embedded formalin fixed placenta tissue blocks were retrieved from the pathology archives. Endocan immunohistochemistry was performed on tissue sections of full thickness and maternal surface of the placenta. The endocan expression was determined in fetal endothelial cells, maternal endothelial cells, cytotrophoblasts, syncytiotrophoblasts and decidual cells. The differences in endocan expression in placenta between hypertensive and normotensive subjects were evaluated by Pearson chi-square test and t-test were used in the statistical analysis.
RESULTS: The endocan expression was significantly higher in fetal endothelial cells (P

Matched MeSH terms: Blood Pressure
The impact of music guided deep breathing exercise on blood pressure control - A participant blinded randomised controlled study

Kow FP, Adlina B, Sivasangari S, Punithavathi N, Ng KK, Ang AH, et al.

Med J Malaysia, 2018 08;73(4):233-238.
PMID: 30121686 MyJurnal

INTRODUCTION: As pharmacological treatment of hypertension has become a burden worldwide, the study looked into nonpharmacological ways of reducing blood pressure. The objective was to determine if music guided, slow and deep breathing will reduce the blood pressure among patients with hypertension in eight weeks.
METHODS: A participant blinded, multi-centre, randomised controlled trial was conducted in which the participants in the intervention group (IG) practiced deep breathing exercise guided by sound cues and those in the control group (CG) listened to the music. The primary end point was reduction in blood pressure at eight weeks.
RESULTS: 87 patients, 46 males and 41 females with mean age of 61.1 years were recruited and 93.1% of them successfully completed the study. There was significant reduction in systolic and diastolic Blood Pressure from baseline by 8 weeks in both groups. The reduction in Mean systolic blood pressure (SBP) in the control arm was 10.5mmHg compared to 8.3mmHg (p<0.001) in intervention group. Diastolic blood pressure (DBP) reduction in control and intervention groups were 5.2 mmHg (p<0.001) and 5.6 mmHg (p<0.001) respectively. The absolute difference in SBP reduction from baseline in IG & CG was -2.2 (95%CI: -7.8 to 3.5) and DBP was -0.4 (95%CI: -2.9 to 3.6). However, blood pressure reduction between the two groups was not significant.
CONCLUSIONS: Both listening to music and deep breathing exercise were associated with a clinically significant reduction in SBP and DBP. However, deep breathing exercise did not augment the benefit of music in reducing BP.

Matched MeSH terms: Blood Pressure
Fulltext Separation and identification of bromelain-generated antibacterial peptides from Actinopyga lecanora

Ghanbari R, Ebrahimpour A

Food Sci Biotechnol, 2018 Apr;27(2):591-598.
PMID: 30263784 DOI: 10.1007/s10068-017-0267-z

Actinopyga lecanora, as a rich protein source was hydrolysed to generate antibacterial bioactive peptides using different proteolytic enzymes. Bromelain hydrolysate, after 1 h hydrolysis, exhibited the highestantibacterial activities against Pseudomonas aeruginosa, Pseudomonas sp., Escherichia coli and Staphylococcus aureus. Two dimensional fractionation strategies, using a semi-preparative RP-HPLC and an isoelectric-focusing electrophoresis, were applied for peptide profiling. Furthermore, UPLC-QTOF-MS was used for peptides identification; 12 peptide sequences were successfully identified. The antibacterial activity of purified peptides from A. lecanora on P. aeruginosa, Pseudomonas sp., E. coli and S. aureus was investigated. These identified peptides exhibited growth inhibition against P. aeruginosa, Pseudomonas sp., E. coli and S. aureus with values ranging from 18.80 to 75.30%. These results revealed that the A. lecanora would be used as an economical protein source for the production of high value antibacterial bioactive peptides.

Matched MeSH terms: Chromatography, High Pressure Liquid
A Protein Decomplexation Strategy in Snake Venom Proteomics

Tan CH, Tan KY, Tan NH

Methods Mol Biol, 2019;1871:83-92.
PMID: 30276733 DOI: 10.1007/978-1-4939-8814-3_5

Snake venoms are complex mixtures of proteins and peptides that play vital roles in the survival of venomous snakes. As with their diverse pharmacological activities, snake venoms can be highly variable, hence the importance of understanding the compositional details of different snake venoms. However, profiling venom protein mixtures is challenging, in particular when dealing with the diversity of protein subtypes and their abundances. Here we described an optimized strategy combining a protein decomplexation method with in-solution trypsin digestion and mass spectrometry of snake venom proteins. The approach involves the integrated use of C18 reverse-phase high-performance liquid chromatography (RP-HPLC), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and nano-electrospray ionization tandem mass spectrometry (nano-ESI-LC-MS/MS).

Matched MeSH terms: Chromatography, High Pressure Liquid
A state-of-the-art review of foot pressure

Zulkifli SS, Loh WP

Foot Ankle Surg, 2020 Jan;26(1):25-32.
PMID: 30600155 DOI: 10.1016/j.fas.2018.12.005

The science of foot pressure studies the forces acting on the bottom and different regions of the foot along with the pressure exerted on the plantar surface with the interacting surface in contact. The information derived gave impact to human biomechanical assessment on body balance and ergonomics posture during gait. Various experiments designed at generating foot pressure data returns only with limited knowledge generated. Obviously, the procedure for experiment design needs to be properly understood from the foot morphology aspects; healthiness, footwear, surface in contact, load and forces impacts, and the foot sensitivity as well as the specification for the foot pressure. This paper reviews the proper preliminary experimental setups for foot pressure measurement analysis during static or dynamic gait. The strength and limitations of recent devices used and considerable variables are also discussed. The overall review explains that the comfortable natural gait in relation to the aspects of sensitivity, load, time duration, and stability are the standard considerations for plantar pressure experiments.

Matched MeSH terms: Pressure
Fulltext Evaluation of the Performance of Helmet Prototypes Fabricated from Acrylonitrile Butadiene Styrene Composites Filled with Natural Resource

Nikmatin S, Hermawan B, Irmansyah I, Indro MN, Kueh ABH, Syafiuddin A

Materials (Basel), 2018 Dec 22;12(1).
PMID: 30583516 DOI: 10.3390/ma12010034

The performance of helmet prototypes fabricated from acrylonitrile butadiene styrene composites filled with oil palm empty fruit bunch fibers was evaluated. The fibers were produced using a milling procedure, while the composites were fabricated using a single-screw extrusion. The physical characteristics of the produced fibers, which are water content, size, and density, were investigated. In addition, the mechanical properties of the produced helmets, including shock absorption, yield stress, frequency, and head injury criterion (HIC), were examined. The impact strength of the produced helmets increases with the rise of filler content. In addition, the helmets were also able to withstand a considerable pressure such that the transmitted pressure was far under the maximum value acceptable by the human skull. The present work also found that HICs exhibited by the investigated helmet prototypes fulfill all the practical guidelines as permitted by the Indonesian government. In terms of novelty, such innovation can be considered the first invention in Indonesia since the endorsement of the use of motorcycle helmets.

Matched MeSH terms: Pressure
Fulltext Enhancement of a protocol purifying T1 lipase through molecular approach

Che Hussian CHA, Raja Abd Rahman RNZ, Thean Chor AL, Salleh AB, Mohamad Ali MS

PeerJ, 2018;6:e5833.
PMID: 30479887 DOI: 10.7717/peerj.5833

T1 Lipase is a thermostable secretary protein of Geobacillus zalihae strain previously expressed in a prokaryotic system and purified using three-step purification: affinity 1, affinity 2, and ion exchange chromatography (IEX). This approach is time consuming and offers low purity and recovery yield. In order to enhance the purification strategy of T1 lipase, affinity 2 was removed so that after affinity 1, the cleaved Glutathione S-transferase (GST) and matured T1 lipase could be directly separated through IEX. Therefore, a rational design of GST isoelectric point (pI) was implemented by prediction using ExPASy software in order to enhance the differences of pI values between GST and matured T1 lipase. Site-directed mutagenesis at two locations flanking the downstream region of GST sequences (H215R and G213R) was successfully performed. Double point mutations changed the charge on GST from 6.10 to 6.53. The purified lipase from the new construct GST tag mutant-T1 was successfully purified using two steps of purification with 6,849 U/mg of lipase specific activity, 33% yield, and a 44-fold increase in purification. Hence, the increment of the pI values in the GST tag fusion T1 lipase resulted in a successful direct separation through IEX and lead to successful purification.

Matched MeSH terms: Chromatography, High Pressure Liquid
Fulltext Polyphenol compounds from pomegranate (Punica granatum) extracted via various methods and its application on meat and meat products: a review

Mohammad Rashedi Ismail-Fitry, Safiullah Jauhar, Chong, Gun Hean, Mahmud Ab Rashid NorKhaizura, Wan Zunairah Wan Ibadullah

Journal of Advanced Research in Applied Sciences and Engineering Technology, 2018;12(1):1-12.
MyJurnal

Recently natural polyphenol compounds (PCs) of plants, gained wide consideration of
scientists, companies and public people because of its unique pharmaceutical and
preservative benefits in the physiological system. They can prevent mortal and serious
diseases such as cancer, cardiovascular and Alzheimer. However, defining a suitable
source of PCs and their proper, economic and efficient extraction method are still a
challenge. The aim of this study was to review PCs as an important antioxidant, the
significance of pomegranate as a source of natural PCs and its application in meat and
meat product. This study also covers different types of PCs extraction methods such as
solid-liquid extraction (SLE) method as a conventional extraction which using Soxhlet
apparatus and several solvents and advanced methods such as microwave-assisted
extraction (MAE), supercritical fluid extraction (SFE), accelerated solvent extraction
(ASE), ultrasound-assisted extraction (UAE) and high hydrostatic pressure extraction
(HHPE). Simple and developed analytical method of PCs is also reviewed in the study.
SLE method is an easy and simple method but, it uses lots of chemicals and is not
suitable for all kinds of PCs extraction. UAE is using for quick extraction PCs, SFE is green
extraction method uses less solvent and have a good result but it needs high
technology. ASE method is a proper alternative for Soxhlet extraction method for its
quick result. MAE method has high extraction result but is not suitable for some
thermolabile PCs. Several parts of pomegranate showed antioxidant and antimicrobial
traits for shelf life extension of meat and meat product. Pomegranate peel is the
strongest antioxidant followed by pomegranate juice and seed. Pomegranate peel
prolonged chilled chicken meat shelf live up to three weeks.

Matched MeSH terms: Hydrostatic Pressure

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