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  1. Wong CL, Sieo CC, Tan WS
    J Virol Methods, 2013 Nov;193(2):611-9.
    PMID: 23933075 DOI: 10.1016/j.jviromet.2013.07.053
    Foot-and-mouth disease (FMD) is a highly contagious epidemic disease threatening the cattle industry since the sixteenth century. In recent years, the development of diagnostic assays for FMD has benefited considerably from the advances of recombinant DNA technology. In this study, the immunodominant region of the capsid protein VP1 of the foot-and-mouth disease virus (FMDV) was fused to the T7 bacteriophage and expressed on the surface of the bacteriophage capsid protein. The recombinant protein of about 42 kDa was detected by the anti-T7 tag monoclonal antibody in Western blot analysis. Phage ELISA showed that both the vaccinated and positive infected bovine sera reacted significantly with the recombinant T7 particle. This study demonstrated the potential of the T7 phage displaying the VP1 epitope as a diagnostic reagent.
  2. Tan OL, Safii SH, Razali M
    Antibiotics (Basel), 2021 Sep 28;10(10).
    PMID: 34680759 DOI: 10.3390/antibiotics10101178
    The aim of this systematic review is to compare the clinical efficacy of repeated applications of local drug delivery and adjunctive agents (LDAs) in nonsurgical periodontal therapy (NSPT) compared to subgingival mechanical debridement (SMD) alone. The Cochrane Central Register of Controlled Trials, MEDLINE, PubMed, EMBASE, Web of Science, hand-searched literature and grey literature databases were searched for randomized controlled clinical trials (RCTs) with a minimum of 6-month follow-up. The outcomes of interest were changes in probing pocket depth and clinical attachment level as well as patient-centred outcomes. Of 1094 studies identified, 16 RCTs were included in the qualitative analysis. Across 11 different adjuncts analysed, only two studies utilizing minocycline gel/ointment and antimicrobial photodynamic therapy (aPDT) with indocyanine green photosensitizer had statistically significant differences in primary outcomes when compared to their control groups. Only one study on aPDT methylene blue 0.005% had compared single versus multiple applications against its control group. A mean range of 0.27-3.82 mm PD reduction and -0.09-2.82 mm CAL gain were observed with repeated LDA application. Considerable clinical heterogeneity and methodological flaws in the included studies preclude any definitive conclusions regarding the clinical efficacy of repeated LDA applications. Future RCTs with a direct comparison between single and repeated applications should be conducted to confirm or refute the clinical advantages of repeated LDA application in the nonsurgical management of periodontitis.
  3. Tan ML, Ho JJ, Teh KH
    Cochrane Database Syst Rev, 2016 Sep 28;9(9):CD009398.
    PMID: 27678554 DOI: 10.1002/14651858.CD009398.pub3
    BACKGROUND: About 5% of school children have a specific learning disorder, defined as unexpected failure to acquire adequate abilities in reading, writing or mathematics that is not a result of reduced intellectual ability, inadequate teaching or social deprivation. Of these events, 80% are reading disorders. Polyunsaturated fatty acids (PUFAs), in particular, omega-3 and omega-6 fatty acids, which normally are abundant in the brain and in the retina, are important for learning. Some children with specific learning disorders have been found to be deficient in these PUFAs, and it is argued that supplementation of PUFAs may help these children improve their learning abilities.

    OBJECTIVES: 1. To assess effects on learning outcomes of supplementation of polyunsaturated fatty acids (PUFAs) for children with specific learning disorders.2. To determine whether adverse effects of supplementation of PUFAs are reported in these children.

    SEARCH METHODS: In November 2015, we searched CENTRAL, Ovid MEDLINE, Embase, PsycINFO, 10 other databases and two trials registers. We also searched the reference lists of relevant articles.

    SELECTION CRITERIA: Randomised controlled trials (RCTs) or quasi-RCTs comparing PUFAs with placebo or no treatment in children younger than 18 years with specific learning disabilities, as diagnosed in accordance with the fifth (or earlier) edition of theDiagnostic and Statistical Manual of Mental Disorders (DSM-5), or the 10th (or earlier) revision of the International Classification of Diseases (ICD-10) or equivalent criteria. We included children with coexisting developmental disorders such as attention deficit hyperactivity disorder (ADHD) or autism.

    DATA COLLECTION AND ANALYSIS: Two review authors (MLT and KHT) independently screened the titles and abstracts of articles identified by the search and eliminated all studies that did not meet the inclusion criteria. We contacted study authors to ask for missing information and clarification, when needed. We used the GRADE approach to assess the quality of evidence.

    MAIN RESULTS: Two small studies involving 116 children, mainly boys between 10 and 18 years of age, met the inclusion criteria. One study was conducted in a school setting, the other at a specialised clinic. Both studies used three months of a combination of omega-3 and omega-6 supplements as the intervention compared with placebo. Although both studies had generally low risk of bias, we judged the risk of reporting bias as unclear in one study, and as high in the other study. In addition, one of the studies was funded by industry and reported active company involvement in the study.None of the studies reported data on the primary outcomes of reading, writing, spelling and mathematics scores, as assessed by standardised tests.Evidence of low quality indicates that supplementation of PUFAs did not increase the risk of gastrointestinal disturbances (risk ratio 1.43, 95% confidence interval 0.25 to 8.15; two studies, 116 children). Investigators reported no other adverse effects.Both studies reported attention deficit hyperactivity disorder (ADHD)-related behaviour outcomes. We were unable to combine the results in a meta-analysis because one study reported findings as a continuous outcome, and the other as a dichotomous outcome. No other secondary outcomes were reported.We excluded one study because it used a cointervention (carnosine), and five other studies because they did not provide a robust diagnosis of a specific learning disorder. We identified one ongoing study and found three studies awaiting classification.

    AUTHORS' CONCLUSIONS: Evidence is insufficient to permit any conclusions about the effect of PUFAs on the learning abilities of children with specific learning disorders. Well-designed RCTs with clearly defined populations of children with specific learning disorders who have been diagnosed by standardised diagnostic criteria are needed.

  4. Cheung KW, Tan LN, Meher S, WHO Intrapartum Care Algorithms Working Group
    BJOG, 2024 Aug;131 Suppl 2:79-89.
    PMID: 35415941 DOI: 10.1111/1471-0528.16726
    AIM: To develop evidence-based clinical algorithms for management of common intrapartum urinary abnormalities.

    POPULATION: Women with singleton, term pregnancies in active labour and immediate postnatal period, at low risk of complications.

    SETTING: Healthcare facilities in low- and middle-income countries.

    SEARCH STRATEGY: A systematic search and review were conducted on the current guidelines from WHO, NICE, ACOG and RCOG. Additional search was done on PubMed and The Cochrane Database of Systematic Reviews up to May 2020.

    CASE SCENARIOS: Four common intrapartum urinary abnormalities were selected: proteinuria, ketonuria, glycosuria and oliguria. Using reagent strip testing, glycosuria was defined as ≥2+ on one occasion or of ≥1+ on two or more occasions. Proteinuria was defined as ≥2+ and presence of ketone indicated ketonuria. Oliguria was defined as hourly urine output ≤30 ml. Thorough initial assessment using history, physical examination and basic investigations helped differentiate most of the underlying causes, which include diabetes mellitus, dehydration, sepsis, pre-eclampsia, shock, anaemia, obstructed labour, underlying cardiac or renal problems. A clinical algorithm was developed for each urinary abnormality to facilitate intrapartum management and referral of complicated cases for specialised care.

    CONCLUSIONS: Four simple, user-friendly and evidence-based clinical algorithms were developed to enhance intrapartum care of commonly encountered maternal urine abnormalities. These algorithms may be used to support healthcare professionals in clinical decision-making when handling normal and potentially complicated labour, especially in low resource countries.

    TWEETABLE ABSTRACT: Evidence-based clinical algorithms developed to guide intrapartum management of commonly encountered urinary abnormalities.

  5. Tan YJ, Linden S, Ong SC
    PLoS One, 2024;19(8):e0305257.
    PMID: 39178204 DOI: 10.1371/journal.pone.0305257
    INTRODUCTION: Empagliflozin demonstrates promising clinical benefits in patients with heart failure (HF). While an early study demonstrates that empagliflozin is cost-effective for treating HF patients with reduced ejection fraction (HFrEF) in Malaysia, its cost-effectiveness for HF with ejection fraction (EF)>40% remains unclear. Therefore, the current study aimed to assess the cost-effectiveness of adding empagliflozin to the standard of care (SoC) for HF patients with EF>40% from the perspective of Malaysian healthcare system. Subsequently, the results were consolidated with the findings for HFrEF to evaluate the cost-effectiveness of empagliflozin when used for all HF patients in Malaysia, irrespective of EF.

    METHODS: A cost-utility analysis was performed using a validated Markov model, which modelled a cohort of adult patients through health states related to symptom severity and functional impairment, to estimate costs and quality-adjusted life-years (QALYs). The influence of model inputs and assumptions, sensitivity, scenario, and subgroup analyses were explored. All costs were expressed in 2022 Malaysian ringgits (RM). Costs and QALYs were discounted at an annual rate of 3.0% as per local pharmacoeconomic guideline.

    RESULTS: The base-case incremental cost-effectiveness ratio (ICER) for HF patients with EF>40% was RM 40,454 per QALY gained. At a cost-effectiveness threshold of RM 47,439/QALY gained, empagliflozin was cost-effective in 57% of replications. The model outcomes were sensitive to inputs related to the treatment effect of empagliflozin in reducing HF-related hospitalisation and cardiovascular mortality, and empagliflozin cost. For the overall HF population, the ICER was RM 29,463/QALY gained.

    CONCLUSION: The findings suggest that empagliflozin is a cost-effective treatment option for the Malaysian HF population, including those with EF>40%. As such, the intervention warrants consideration by the Malaysian healthcare provider to mitigate the burden of HF and address the unmet needs of the EF>40% population.

  6. Tan ECH, Weng Onn S, Montalvo S
    J Strength Cond Res, 2024 Sep 01;38(9):e529-e533.
    PMID: 38953840 DOI: 10.1519/JSC.0000000000004854
    Erik, HT, Onn, SW, and Montalvo, S. Vertical jump height with artificial intelligence through a cell phone: a validity and reliability report. J Strength Cond Res 38(9): e529-e533, 2024-This study estimated the reliability and validity of an artificial intelligence (AI)-driven model in the My Jump 2 (My Jump Lab ) for estimating vertical jump height compared with the Force Platform (FP). The cross-sectional study involved 88 athletes (33 female and 55 male athletes), performing a total of 264 countermovement jumps with hands on hips. "Jump heights were simultaneously measured using the FP and the My Jump 2 app." The FP estimated jump heights using the impulse-momentum method, whereas My Jump 2 used the flight-time method, with the latter using an AI feature for automated detection of jump take-off and landing. Results indicated high reliability for the AI model (intraclass correlation coefficient [ICC 1,3 ] = 0.980, coefficient of variation [CV] = 4.12) and FP (ICC 1,3 = 0.990, CV = 2.92). Validity assessment showed strong agreement between the AI model and FP (ICC 2,k = 0.973). This was also supported by the Bland-Altman analysis, and the ordinary least products regression revealed no significant systematic or proportional bias. The AI-driven model in My Jump 2 is highly reliable and valid for estimating jump height. Strength and conditioning professionals may use the AI-based mobile app for accurate jump height measurements, offering a practical and efficient alternative to traditional methods.
  7. Kiruthika S, Tan LF, Foo CN
    PMID: 39156233 DOI: 10.51866/oa.521
    INTRODUCTION: Differentiating between migraine and COVID-19 headaches is essential for better treatment. Evidence-based research during the COVID-19 pandemic has found that university students are more likely to experience migraine. Migraine can affect academic performance, sleep pattern, social and emotional well-being if left untreated or misdiagnosed. This study aimed to determine the prevalence of migraine symptoms and the association of triggers, coping strategies and clinical characteristics with COVID-19 diagnosis.

    METHODS: This cross-sectional study was conducted across higher educational institutions in Peninsular Malaysia. Convenience sampling was applied to recruit full-time university students. A reliable and validated instrument was used to evaluate demographic data, migraine symptoms, triggers, coping strategies and clinical characteristics of migraine (frequency, intensity, severity and duration) during COVID-19 diagnosis.

    RESULTS: The response rate was 98.3%, where 485 out of 493 responses were analysed. The prevalence of migraine was 35.9% (n=174). None of the triggers, coping strategies and clinical characteristics of migraine were significantly associated with COVID-19 diagnosis.

    CONCLUSION: The university students in Peninsular Malaysia showed a considerable prevalence of migraine symptoms. During the pandemic, the common triggers for existing symptoms were stress and a lack of sleep (combined triggers). The coping strategy adopted by most of the university students was lifestyle changes and in the COVID-19 positive group maladaptive coping strategies were adopted indicating the need for further investigation.

  8. Tan YJ, Ong SC, Low JZ
    Clin Ther, 2024 Sep 10.
    PMID: 39261261 DOI: 10.1016/j.clinthera.2024.08.008
    PURPOSE: This is a budget impact analysis that compared the scenario of treating heart failure with reduced ejection fraction (HFrEF) using dapagliflozin plus standard of care (SoC) versus a scenario without dapagliflozin, from the perspective of Ministry of Health (MOH) Malaysia over a 5-year time horizon.

    METHODS: A Microsoft Excel-based cost calculator was developed for such comparison. The estimated size of eligible population, uptake rates for dapagliflozin, as well as costs related to drugs, clinical events, and adverse events were based on published data, official tariffs, and databases, and expert opinion. Clinical data from the DAPA-HF trial were used to inform efficacy and safety inputs (i.e., hospitalization for heart failure (hHF), cardiovascular death, and adverse events). Results were reported as total annual and cumulative costs (in 2023 Malaysian Ringgits [RM], United States Dollars [USD], and European Union Euros, [EUR]; with exchange rates of 1 USD = RM 4.40 and 1 EUR = RM 4.90]), as well as the number of clinical events. Sensitivity and scenario analyses were also conducted.

    FINDINGS: The base-case analysis estimated that over a five-year period, the adoption of dapagliflozin for HFrEF treatment would result in a cumulative cost-saving of RM 2.6 million (USD 0.6 million/EUR 0.5 million), representing a 0.3% reduction in costs, driven primarily by reduced expenditure on hHF. Moreover, dapagliflozin treatment would lead to 731 fewer hHF and 366 fewer cardiovascular deaths. Sensitivity and scenario analyses revealed that the results were most sensitive to assumptions regarding loop diuretic requirements and the cost of dapagliflozin. Although cost savings or a net-zero balance were projected for the first four years, an anticipated 2.5% annual increase in dapagliflozin uptake in the longer term would lead to additional costs for the MOH, starting from the fifth year.

    IMPLICATIONS: Incorporating dapagliflozin into the SoC can improve health outcomes for HFrEF patients and may generate cost savings, potentially easing the economic strain of HFrEF management on Malaysia's public healthcare system in the short term. Nonetheless, a modest increase in budget should be anticipated as more patients gain access to the treatment over time.

  9. Aye LM, Tan JEH, Ramasamy S
    J Public Health Afr, 2024;15(1):562.
    PMID: 39229342 DOI: 10.4102/jphia.v15i1.562
    BACKGROUND: Following the implementation of the Movement Control Order (MCO) during the coronavirus disease 2019 (COVID-19) pandemic, academicians from the universities in Malaysia needed to ensure that the quality-of-service delivery to the stakeholders is undisturbed by adopting new challenging norms. This compromises the work-life balance (WLB), causes more stress and potentially affects their quality of life (QoL).

    AIM: This study investigates how perceived stress (PS) impacts the QoL of Malaysian academicians during the COVID-19 pandemic, focusing on the mediating role of WLB.

    SETTING: Academics working in Malaysia during COVID-19 pandemic.

    METHODS: A cross-sectional study, using a voluntary response sampling method, was conducted among 417 academicians from universities in Malaysia in September 2021. A self-reported online questionnaire, measuring PS, WLB and QoL, was distributed.

    RESULTS: The QoL scored a mean of 50 (standard deviation [s.d.] = 9.84), PS scored a mean of 24.26 (s.d. = 8.19) and WLB had a mean score of 51.12 (s.d. = 18.73). Work-life balance was a significant mediator of PS and QoL (β = -0.43, 95% confidence interval [CI] = -0.52 to -0.35, p = 0.0001). Perceived stress was a significant predictor of WLB (β = 1.62, p = 0.0001).

    CONCLUSION: Institutions should consider implementing flexible working arrangements, and providing workshops on crisis management, time management, and resilience. Stress coping methods are recommended for enhancing WLB among academicians.

    CONTRIBUTION: This study contributes to the pool of evidence to support intervention strategies and policy recommendations aimed to enhance well-being.

  10. Lan H, Bailey R, Tan WH
    Heliyon, 2024 Aug 30;10(16):e35675.
    PMID: 39220952 DOI: 10.1016/j.heliyon.2024.e35675
    Rapid growth has been witnessed in digital technologies, resulting in significant productive changes in communication and information technology-oriented practices in different fields. Hybrid teaching or blended learning has gained progressively widespread acceptance in the educational process, and concerns about teachers' information and communication technology (ICT) competence or digital competence have become prevalent. This study adopts a systematic review to interpret and analyse the relevant research findings, aiming to systematically investigate the existing models and paradigms of ICT competence or digital capacities and to understand further how these models and paradigms are applied specifically within the context of Chinese universities. Based on inclusion and exclusion criteria, 12 out of 801 candidate studies were accessed from Web of Science, Scopus and the China National Knowledge Infrastructure (CNKI) databases. The findings indicate that the Technological Pedagogical Content Knowledge (TPACK) and the National Educational Technology Guides for Teachers in Higher Education (CETG) are frequently used to evaluate college educators' digital proficiency or ICT capability in China. Educators' demographic characteristics, self-efficacy, external factors such as institutional policies and the availability of infrastructure and training collectively influence how digital teaching is integrated within Chinese higher education institutions. By conducting a systematic review of research trends and the critical factors affecting the digital competence of faculty in Chinese universities, this study aims to equip policymakers and educators with a deeper understanding of how to prepare for educational shifts in the digital era and thereby ensure the sustainability of professional development.
  11. Tan JH, Liew KJ, Goh KM
    Data Brief, 2024 Oct;56:110829.
    PMID: 39252782 DOI: 10.1016/j.dib.2024.110829
    This data report presents prokaryotic metagenome-assembled genomes (MAGs) from a hot spring stream with temperatures between 64 and 100°C. The stream water was filtered and the extracted total DNA was sequenced using the Illumina HiSeq 2500 platform. Approximately 80 Gb of raw data were generated, which were subsequently assembled using MEGAHIT v1.2.9. The MAGs were generated using MetaWRAP with binning approaches of MetaBAT2, CONCOCT and MaxBin2. We constructed 25 medium-quality and 24 high-quality archaeal MAGs, and 152 medium-quality and 112 high-quality bacterial MAGs. The fasta files of these MAGs are available in the NCBI database as well as Mendeley Data. Major phyla identified include Bacteroidota, Chloroflexota, Desulfobacterota, Firmicutes, Patescibacteria, Proteobacteria, Spirochaetota, Verrucomicrobiota, Armatimonadota, Nitrospirota, Acidobacteriota, Elusimicrobiota, Planctomycetota, Candidate division WOR-3, Aquificota, Thermoproteota, and Micrarchaeota. This dataset is valuable for studies on thermophilic genomes, reconstruction of biochemical pathways and gene discovery.
  12. Wong YP, Tan GC, Khong TY
    APMIS, 2024 Nov 07.
    PMID: 39509086 DOI: 10.1111/apm.13496
    The 2016 Amsterdam Placental Workshop Group Consensus Statement recommends sampling a block of the placenta close to the umbilical cord insertion site (UCIB) for histopathological evaluation. This piece of placenta at the umbilical cord insertion is presumed to give a better yield of inflammation (if present). We aimed to investigate the utility of the UCIB in the detection of maternal and/or fetal inflammatory responses (MIR and/or FIR), in comparison with the other sections of the placental parenchyma. This is a retrospective cross-sectional study including all placentas with histologic chorioamnionitis. The histopathological slides of placentas were reviewed as per Amsterdam consensus guidelines. Diagnostic performance of UCIB in identifying MIR and/or FIR, relative to the other placental sections, was assessed. UCIB revealed diagnostic sensitivity, specificity, and diagnostic accuracy of 79.2% (95% CI: 74.2-83.6%), 100.0% (95% CI: 95.6-100.0%), and 83.6% (95% CI: 79.5-87.2%), respectively, in the detection of FIR, while showing a low sensitivity of 52.6% (95% CI: 47.5-57.6%) in detecting MIR. In 59 (24.6%) cases, FIR was not seen in the corresponding placental parenchymal sections but was detected in the UCIBs. This study is the first study to confirm that a section from the UCIB is essential for the detection of FIR, which affirms the Amsterdam consensus sampling recommendations.
  13. Radzliyana Radzuwan, Nadiah Diyana Tan Abdullah, Nasyitah Tp Harun
    MyJurnal
    Today sport and tourism are among the developed worlds’ most sought after leisure experiences and are becoming very important reflecting the hos t venue both socially and culturally. In this new era, many challenges were even more significant for Malaysia as she is not yet developed like other countries. Therefore, a careful selection of a potential place as a host venue for sports’ event is import an t. In line with that notion, this study aims to examine residents’ perception towards social and cultural impacts on hosting World Kite Festival in Pasir Gudang, Johor. A self administered questionnaire was developed. A total of 461 residents were succes sf ully surveyed and their perceptions towards social and cultural impacts were assessed by utilizing Scale of Perceived Social Impact (SPSI). All obtained data were further analyzed by using Statistical Package for Social Science (SPSS). In order to achiev e the research objectives, descriptive statistic was used for assessing the percentages, mean, rank and standard deviation. While independent t Test was used to investigate the gender differences among residents at World Kite Festival. The finding indicate s that there was a positive impact of hosting a sports’ event to the resident at Pasir Gudang although some negative impacts were witnessed. Thus, some improvement is needed.
  14. Tan TS, Sharifah Syed Hassan, Yap WB
    Sains Malaysiana, 2016;45:787-793.
    The use of cell lines such as Madin-Darby Canine Kidney (MDCK) and African Green Monkey Kidney (Vero) cells in
    influenza vaccine production is much advocated presently as a safer alternative to chicken embryonated eggs. It is
    thus essential to understand the influenza virus replication patterns in these cell lines prior to utilizing them in vaccine
    production. The infectivity of avian influenza A virus (A/Chicken/Malaysia/5858/2004) H5N1 in MDCK and Vero cell
    lines was first assessed by comparing the cytopathic effect (CPE) caused by the virus infection. The viral loads in both
    of the infected media and cells were also compared. The results showed that both of the MDCK and Vero cells began to
    exhibit significant CPE (p<0.05) after 48 h post-infection (h p.i). The MDCK cell line was more susceptible to the virus
    infection compared to Vero cell line throughout the incubation period. A higher viral load was also detected in the host
    cells compared to their respective culturing media. Interestingly, after reaching its maximum titer at 48 h p.i, the viral
    load in MDCK cells declined meanwhile the viral load in Vero cells increased gradually and peaked at 120 h p.i. Overall,
    both cell lines support efficient H5N1 virus replication. While the peak viral loads measured in the two cell lines did
    not differ much, a more rapid replication was observed in the infected MDCK samples. The finding showed that MDCK
    cell line might serve as a more time-saving and cost-effective cell culture-based system compared to Vero cell line for
    influenza vaccine production.
  15. Tan Hc, Tan Bc, Norzulaani Khalid, Wong Sm
    Sains Malaysiana, 2016;45:795-802.
    Boesenbergia rotunda is a medicinal ginger that has been found to contain several bioactive compounds such as
    boesenbergin A, panduratin A, cardamonin, pinostrobin and pinocembrin. These compounds are useful in treating various
    ailments, such as oral diseases, inflammation and have also been used as an aphrodisiac. In this study, an efficient
    protocol for developing and isolating protoplast cultures for B. rotunda has been established. Rhizome buds of B. rotunda
    were used as explants to initiate callus growth and the established cell suspension cultures were used to optimize their
    growth conditions. Our results indicated that embryogenic suspension cultures in liquid Murashige and Skoog (MS)
    medium supplemented with 3% (w/v) sucrose produced the highest growth rate (µ = 0.1125), whereas no promotive effect
    was seen in the presence of 2,4-dichlorophenoxyacetic acid and those that underwent sonication treatment. Amount of
    protoplasts isolated ranging from 1-5 × 105
    protoplast per mL were isolated using 0.25% (w/v) macerozyme and 1%
    (w/v) cellulase for 24 h under continuous agitation (50 rpm) in dark condition. Of the isolated protoplasts, 54.93% were
    viable according to fluorescein diacetate staining test. Micro-colonies were recovered in liquid MS medium containing
    9 g/L mannitol, 2 mg/L 1-naphthaleneacetic acid and 0.5 mg/L benzylaminopurine (BAP) for 4 weeks and subsequently
    transferred to solid MS medium supplemented with 0.5 mg/L BAP for callus initiation. The protoplast system established
    in this study would be useful for genetic manipulation and modern breeding program of B. rotund
  16. Ahmad Firdaus Khalid, Yoke KY, Jun JT
    Sains Malaysiana, 2018;47:2705-2711.
    The use of honey as a therapeutic agent dates back at 8000 years and has markedly increased interest into its potential
    health benefits. The by-products of the flower nectar have a complex chemical composition which promotes benefits in
    underlying mechanism of human diseases. Malaysian Tualang Honey (MTH) is a multifloral jungle honey produced by
    the rock bee (Apis dorsata). This review consolidates the results of carious studies involving biochemical assays of tissue
    culture and animal trials of anti-cancer properties of MTH. Often studied in the context of breast cancer cell lines, MTH
    has promising data for possible mechanisms in anti-cancer activity. These include apoptosis via depolarization of the
    mitochondrial membrane, caspase-dependent apoptosis, reduction of angiogenesis and the promotion of cell cycle arrest
    without posing cytotoxic effect on normal cell lines. Despite positive outcomes in tissue cultures, the oral administration
    of MTH in breast cancer animal models showed slower tumour progression, reduction in tumour size and better grading
    of histological features. The alleviation of breast carcinogenesis via modulation of hematologic, estrogenic and apoptotic
    activities promotes MTH as a promising anticancer agent. With confidence in a conclusion that MTH is a useful treatment
    for cancer, further experimental and clinical studies should be conducted.
  17. Nor’ain Senin, Noorhidayah Ramli, Mai Noor Asiah Tan Zalilah
    MyJurnal
    A new innovation has been created which is called AUTOMATIC MEAT SLICING (AMS) MACHINE. The main purpose of the machine creation is to replace the old-style method in slicing the chicken meat to modern method. BABARITTOS DELIGHT supplies Tortillas Wrap to the latest trend of Food & Beverage (F&B) business called Food Truck. The main ingredient of Burritos is slices of boneless chicken meat, where each slice is approximately has to be 5 mm thick. Formerly, the slicing procedure used a normal cutting knife and its production rate was truncated as the demands of the Burritos are going higher by days. This method requires 3 to 4 workers to team up to complete the 30 kg boneless chicken meat to be cut into roughly 5 mm per slice within one and half hour. The AMS machine is predominant to help the company to grow the output of sliced meat and at the same time can reduce the number of workers. The usage of AMS machine is only required one worker for the operation and the output can be up to 30kg per 30mins, which is actually can end up almost 3 times quantity of output and 3 times labor cost-saving. Meanwhile the quality of the sliced meat is much enhanced as most of the slice meat is precisely 5mm thick. The AMS concept design is cutting the meat using several circular cutting blades, where the chicken will be put on the moving conveyor and finally will fall onto the hygienic tray.
  18. Ho, L.H., Zainal Abidin, N.F.S., Tan, T.C., Noroul Asyikeen, Z.
    MyJurnal
    The effect of partial substitution of pumpkin flour for rice flour on the physical properties and sensory attributes of gluten-free muffin were investigated. Pumpkin flour was used to replace 10, 15 and 20% rice flour in a control gluten-free muffin formulation (without pumpkin flour). The partial substitution of pumpkin flour for rice flour did not affect moisture content of gluten-free muffins. However, the pumpkin flour substitution caused significant reduction in water activity of gluten-free muffins. Results on the volume, specific volume and height of all gluten-free muffins showed no significant effect with the increasing percentage of pumpkin flour substitution. However, pumpkin flour substitution significantly reduced the firmness of composite muffins, and improved its springiness. The colour of crumb progressively became darker as the level of pumpkin flour substitution increased. Moreover, the results also showed that the substitution of pumpkin flour caused an increase in yellowness (b*) value of crust and crumb of gluten-free muffin. Sensory evaluation indicated that all gluten-free muffins incorporated with pumpkin flour received similar score when compared to that of control.
  19. Lip HTC, Lip HTC, Lip HTC, Huei TJ, Huei TJ, Huei TJ, et al.
    J Coll Physicians Surg Pak, 2019 Feb;29(2):185-186.
    PMID: 30700363 DOI: 10.29271/jcpsp.2019.02.185
    This is a retrospective audit of all patients admitted to the surgical unit for urosepsis from June 2014 to June 2015 at the General Surgery Unit of Hospital Sultan Ismail, Malaysia. Demographics, comorbidities, presenting symptoms, length of stay (LOS), mortality, and associated risk factors were recorded. There were 35 patients treated for urosepsis with a male preponderance of 21/35 patients. Hypertension (n=18) and diabetes (n=10) were the two most common comorbidities. There were five deaths (mortality rate=14.3%) and the main bacterium cultured was Escherichia coli. Age >65, presence of underlying comorbid disease, presence of cancer, urine and blood culture positivity had higher incidence of death but were not statistically significant for urosepsis mortality. However, this result is limited by the small sample size and single centre retrospective data.
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