Browse publications by year: 2025

  1. Yeo BS, Lee WX, Mahmud R, Tan GC, Wahid MIA, Cheah YK
    World J Oncol, 2025 Feb;16(1):1-15.
    PMID: 39850528 DOI: 10.14740/wjon1955
    The investigation of microRNAs (miRNAs) for the purpose of identifying biomarkers and new treatments for breast cancer has been gaining traction from scientists in recent years. Of all the miRNAs, miR-155 has been reportedly involved in breast cancer development as it regulates various cellular processes such as glucose uptake, proliferation, metastasis, and migration. Various efforts have been done towards researching miR-155 as a biomarker in breast cancer; however, the results were varied. The objective of the current systematic review is to compile and summarize information regarding miR-155 as a potential diagnostic biomarker for breast cancer. All eligible studies were found from SCOPUS and PubMed databases. Out of the 376 potential eligible records, only 26 original articles were selected for further assessment according to inclusion and exclusion criteria. The expressions of miR-155 in serum, plasma, biopsy, urine, nipple aspirate fluid, serum exosomes, and peripheral blood mononuclear cells were recorded and analyzed. Besides that, the expression of miR-155 was also correlated to clinicopathological features in breast cancer patients. The area under the curve (AUC) values from receiver operating characteristic (ROC) analysis used to evaluate diagnostic sensitivity and specificity of miR-155 as a diagnostic biomarker were also recorded. The limitations such as the small sampling size, the unemployment of internal controls for quantitative real-time polymerase chain reaction (RT-qPCR), and inconsistency of sensitivity as well as specificity values of miR-155 as a biomarker have been discussed. The present study proposed that miR-155 is a good diagnostic biomarker for breast cancer; however, further clinical research is required to assess the validity of miR-155 as a potential biomarker to translate the research outcomes into clinical practice.
  2. Alyas S, Hussain R, Ababneh BF, Ong SC, Babar ZU
    Explor Res Clin Soc Pharm, 2025 Mar;17:100558.
    PMID: 39850827 DOI: 10.1016/j.rcsop.2024.100558
    BACKGROUND: Asthma self-management is an effective approach that empowers patients with asthma to control their condition and reduce its impact on their daily lives.

    OBJECTIVE: This systematic review aims to synthesize evidence regarding the knowledge, perceptions, facilitators, and barriers related to asthma self-management among patients.

    METHODS: A systematic literature search was conducted across five databases (PubMed, Science Direct, Scopus, Web of Science, and Google Scholar) using specific key terms. Studies that reported knowledge, perceptions, facilitators and barriers towards asthma self-management were included. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were followed to report this review. All the data from included studies were analyzed through narrative synthesis approach.

    RESULTS: A total of 17 studies were included in this review. The findings indicated that most patients had inadequate knowledge of asthma self-management, particularly regarding asthma fundamentals, and inhaler techniques. Patients' cultural beliefs, and perceived social stigma influenced their self-management practices. Facilitators of asthma self-management included strong partnerships with healthcare providers, social support, access to medication, and having a written asthma action plan. Conversely, poor communication with healthcare professionals, travel, smoking, and workplace challenges were identified as barriers.

    CONCLUSION: There is a pressing need for education and training programs to enhance understanding of the disease, and inhaler technique in patients with asthma. Healthcare professionals should create tailored asthma action plans according to patients' beliefs and needs. Moreover, healthcare policies should be developed to promote facilitators and address barriers, to ensure effective asthma management.

  3. Lee OW, Gao D, Peng T, Wunderlich J, Mao D, Balasubramanian G, et al.
    Trends Hear, 2025;29:23312165241311721.
    PMID: 39850978 DOI: 10.1177/23312165241311721
    This study used functional near-infrared spectroscopy (fNIRS) to measure aspects of the speech discrimination ability of sleeping infants. We examined the morphology of the fNIRS response to three different speech contrasts, namely "Tea/Ba," "Bee/Ba," and "Ga/Ba." Sixteen infants aged between 3 and 13 months old were included in this study and their fNIRS data were recorded during natural sleep. The stimuli were presented using a nonsilence baseline paradigm, where repeated standard stimuli were presented between the novel stimuli blocks without any silence periods. The morphology of fNIRS responses varied between speech contrasts. The data were fit with a model in which the responses were the sum of two independent and concurrent response mechanisms that were derived from previously published fNIRS detection responses. These independent components were an oxyhemoglobin (HbO)-positive early-latency response and an HbO-negative late latency response, hypothesized to be related to an auditory canonical response and a brain arousal response, respectively. The goodness of fit of the model with the data was high with median goodness of fit of 81%. The data showed that both response components had later latency when the left ear was the test ear (p 
    MeSH terms: Age Factors; Brain/physiology; Discrimination (Psychology)/physiology; Female; Humans; Infant; Male; Oxyhemoglobins/metabolism; Predictive Value of Tests; Reaction Time
  4. Step K, Eltaraifee E, Elsayed I, Rasaholiarison N, Okubadejo N, Walker R, et al.
    Mov Disord, 2025 Jan;40(1):51-56.
    PMID: 39482233 DOI: 10.1002/mds.30051
  5. Armstrong D, Hungin AP, Kahrilas PJ, Sifrim D, Moayyedi P, Vaezi MF, et al.
    Aliment Pharmacol Ther, 2025 Feb;61(4):636-650.
    PMID: 39740235 DOI: 10.1111/apt.18420
    BACKGROUND: Many patients diagnosed with gastro-oesophageal reflux disease (GERD) have persistent symptoms despite proton pump inhibitor (PPI) therapy.

    AIMS: The aim of this consensus is to provide evidence-based statements to guide clinicians caring for patients with refractory reflux-like symptoms (rRLS) or refractory GERD.

    METHODS: This consensus was developed by the International Working Group for the Classification of Oesophagitis. The steering committee developed specific PICO questions pertaining to the management of PPI rRLS. Methodologists conducted systematic reviews of the literature. The quality of evidence and strength of recommendations were rated using the GRADE approach.

    RESULTS: Consensus was reached on 13 of 17 statements on diagnosis and management. For rRLS, suggested diagnostic strategies included endoscopy, ambulatory reflux testing and oesophageal manometry. The group did not reach consensus on the role of oesophageal biopsies or the use of reflux-symptom association in patients undergoing reflux testing. The group suggested against increasing the PPI dose in patients who had received 8 weeks of a twice-daily PPI. Adjunctive alginate or antacid therapy was suggested. There was no consensus on the role of adjunctive prokinetics. There was little role for adjunctive transient lower oesophageal sphincter relaxation (TLESR) inhibitors or bile acid sequestrants. Endoscopic or surgical anti-reflux procedures should not be performed in patients with rRLS in the absence of objectively confirmed GERD.

    CONCLUSIONS: The management of rRLS should be personalised, based on shared decision-making regarding the role of diagnostic testing to confirm or rule out GERD as a basis for treatment optimisation. Anti-reflux procedures should not be performed without objective confirmation of GERD.

    MeSH terms: Antacids/therapeutic use; Humans; Manometry/methods; Evidence-Based Medicine; Consensus*
  6. Chan A, Chan D, Ng DQ, Zheng HF, Tan QM, Tan CJ, et al.
    Integr Cancer Ther, 2025;24:15347354251314514.
    PMID: 39840742 DOI: 10.1177/15347354251314514
    INTRODUCTION: As pharmacological strategies remain limited for relieving fatigue and associated cognitive symptoms, integrative modalities such as traditional Chinese medicine (TCM) could be explored as therapeutic strategies in cancer survivors. Here, we evaluate and report the efficacy and safety of a TCM concoction, modified Xiang Bei Yang Rong Tang (XBYRT), on quality of life (QOL), cancer-related fatigue (CRF), and cognitive symptoms, compared to placebo.

    METHODS: In a single-centered, randomized, double-blinded, placebo-controlled pilot trial conducted from 2019 to 2022, fatigued cancer survivors ≥21 years old were recruited to receive the XBYRT intervention or placebo (5% diluted) once daily for the duration of 8 weeks. Patient-reported outcomes for QOL, CRF, cognition, blood samples for biomarker testing, and adverse events were collected at baseline (T0), 4 weeks (T1), 8 weeks (T2), and 10 weeks (T3) after baseline. Linear regression was performed to evaluate differences between groups at T2 and T3.

    RESULTS: A total of 1502 patients were screened, with 672 patients considered eligible. Of the eligible, 15 XBYRT and 13 placebo subjects with similar mean ages (58.5 vs 58.4) were recruited. Both groups were predominantly Chinese (93% vs 62%), breast cancer patients (87% vs 62%), and diagnosed with stage 2 cancer (60% vs 46%). Although no significant difference was found in QOL between groups, the XBYRT group exhibited improved emotional fatigue at T3 (P = .045) and higher BDNF levels at T2 (P = .047) and T3 (P = .029). After baseline adjustment, XBYRT was associated with better perceived cognitive impairment at T2 (P = .011) and T3 (P = .017), as well as overall perceived cognitive function at T3 (P = .028). XBYRT is well tolerated, with grade 3 adverse events reported in three XBYRT (20%) and two placebo (15%) subjects.

    CONCLUSION: In this pilot study, XBYRT as an integrative therapy is safe and generates encouraging improvements in cognitive and fatigue symptoms. Difficulties with recruitment limited the generalizability of trial findings, thus findings should be verified through a larger, multi-centered trial.

    MeSH terms: Adult; Aged; Cognition/drug effects; Cognition/physiology; Double-Blind Method; Female; Humans; Male; Middle Aged; Neoplasms/complications; Neoplasms/drug therapy; Neoplasms/psychology; Pilot Projects; Quality of Life*
  7. Mustafa NF, Cheng KK, Razali SA, Wahab HA, Salin NH, Zakaria II, et al.
    Mol Divers, 2025 Jan 22.
    PMID: 39841317 DOI: 10.1007/s11030-024-10899-5
    Dengue is one of the most prevalent viruses transmitted by the Aedes aegypti mosquitoes. Currently, no specific medication is available to treat dengue diseases. The NS2B-NS3 protease is vital during post-translational processing, which is a key target in this study. Due to methoxy group substitution, methoxyflavones are more bioavailable and metabolically stable than hydroxylated flavones. To date, research on the anti-dengue activity of methoxyflavones is limited. Hence, this work aims to determine the inhibitory activity of methoxyflavones against the dengue NS2B-NS3. Methoxyflavones derivatives were screened using molecular docking. The result showed a strong binding interaction of compound 1 and compound 2 with NS2B-NS3 protease. Both compounds exhibited comparable binding energy as the reference compound, quercetin, with values lower than - 8.1 kcal/mol. Molecular dynamics simulation using GROMACS revealed the stability and stiffness of the complexes over a 100 ns time scale. In addition, an in vitro assay for NS2B-NS3 protease inhibition revealed inhibitory effects of compounds 1 and 2 with IC50 values of 316.80 µM and 463.30 µM, respectively. The ADMET analyses showed favorable pharmacokinetics profiles that comply with Lipinski's Rule of Five. Collectively, our findings suggest that compounds 1 and 2 inhibit dengue NS2B-NS3 activity. These findings hold promise of methoxyflavones as starting compounds for potential dengue treatment, highlighting the need for further investigation.
  8. Fan X, Soh KG, Mun CY, Soh KL
    Aging Clin Exp Res, 2025 Jan 22;37(1):32.
    PMID: 39841325 DOI: 10.1007/s40520-024-02894-5
    BACKGROUND: Tai Chi (TC) is widely acknowledged for its positive impact on improving motor function in older adults. Nevertheless, limited research has directly compared the effects of different TC styles on older adults with functional impairments.

    OBJECTIVE: This study aimed to assess the impact of different TC styles on motor function in older adults with functional impairments.

    METHOD: We searched five databases-PubMed, Scopus, Chinese National Knowledge Infrastructure (CNKI), Web of Science, and Wiley Online Library-including studies published up to September 2024. The selection of literature adhered to PRISMA guidelines, with quality assessment independently carried out by two researchers.

    RESULTS: Fourteen studies met the inclusion criteria for this review. The analysis revealed that TC interventions for functionally impaired older adults primarily employed Yang-style, Sun-style, Chen-style, and simplified-style TC. The populations studied included individuals with mild cognitive impairment (MCI), nonspecific low back pain (NS-LBP), preclinical disabilities, chronic diseases, poor balance, osteoarthritis (OA), Parkinson's disease (PD), sarcopenia, and those at risk of falls. The findings indicated that motor function in functionally impaired older adults were closely linked to balance, gait, mobility, strength, and fall rates. Among the various TC styles, Yang-style was the most frequently utilised intervention.

    CONCLUSION: This review examined four types of TC interventions and found strong evidence supporting the effectiveness of Yang-style TC in improving motor function in older adults with functional impairments. Additionally, five assessment methods-Single-Leg Stance (SL), Six-Minute Walk Test (6MWT), Timed Up and Go Test (TUGT), Chair Stand Test (CST), and Fall Efficacy Scale (FES)-were identified as suitable for evaluating this population. Based on the findings, it is recommended that individuals with functional impairments engage in Yang-style 24-movement TC, with an intervention duration of 12 weeks, practicing two to five times a week for 60 min each session.

    MeSH terms: Accidental Falls/prevention & control; Aged; Gait/physiology; Humans
  9. Yusoff NA, Abd Hamid Z, Taib IS, Abdul Razak SR, Budin SB
    Adv Exp Med Biol, 2025 Jan 23.
    PMID: 39841383 DOI: 10.1007/5584_2024_846
    Epigenetic regulation in hematopoietic stem cells (HSCs) research has emerged as a transformative molecular approach that enhances understanding of hematopoiesis and hematological disorders. This chapter investigates the intricate epigenetic mechanisms that control HSCs function, including deoxyribonucleic acid (DNA) methylation, histone modifications, and chromatin remodeling. It also explores the role of non-coding ribonucleic acid (RNAs) as epigenetic regulators, highlighting how changes in gene expression can occur without alterations to the DNA sequence. Epigenetic mechanisms play a pivotal in regulating HSC self-renewal and differentiation, processes essential for maintaining a balanced hematopoietic system in which lineage-specific hematopoietic stem and progenitor cells (HSPCs) pool is sustained. Recent advancements in epigenetic mapping and sequencing technologies have illuminated the dynamic epigenetic landscapes that characterize HSCs and their progeny. Numerous studies have revealed that dysregulation of epigenetic pathways is a hallmark of various hematological malignancies, including leukemias, lymphomas, and myelodysplastic syndromes. This review highlights key findings that demonstrate the impact of epigenetic abnormalities on the disruption of HSPC niches and the progression of oncogenesis in hematological malignancies. Furthermore, this chapter explores the therapeutic potential of targeting epigenetic modifications that are critical in formation and progression of hematologic malignancies. It also discusses the latest developments in epigenetic therapies, including the use of DNA methyltransferase inhibitors, histone deacetylase inhibitors, and emerging drugs targeting other epigenetic regulators. These therapies represent a promising strategy for resetting aberrant epigenetic states, potentially restoring normal hematopoiesis. Conclusively, this chapter offers a thorough overview of the current landscape and future directions of epigenetic research related to the maintenance of the HSPC niches. The insights presented here aim to contribute significantly to the field, offering a reference point for molecular approaches that enhance our understanding of hematopoiesis and its associated hematological malignancies.
  10. Shah DP, Thaweethai T, Karlson EW, Bonilla H, Horne BD, Mullington JM, et al.
    JAMA Netw Open, 2025 Jan 02;8(1):e2455430.
    PMID: 39841477 DOI: 10.1001/jamanetworkopen.2024.55430
    IMPORTANCE: A substantial number of individuals worldwide experience long COVID, or post-COVID condition. Other postviral and autoimmune conditions have a female predominance, but whether the same is true for long COVID, especially within different subgroups, is uncertain.

    OBJECTIVE: To evaluate sex differences in the risk of developing long COVID among adults with SARS-CoV-2 infection.

    DESIGN, SETTING, AND PARTICIPANTS: This cohort study used data from the National Institutes of Health (NIH) Researching COVID to Enhance Recovery (RECOVER)-Adult cohort, which consists of individuals enrolled in and prospectively followed up at 83 sites in 33 US states plus Washington, DC, and Puerto Rico. Data were examined from all participants enrolled between October 29, 2021, and July 5, 2024, who had a qualifying study visit 6 months or more after their initial SARS-CoV-2 infection.

    EXPOSURE: Self-reported sex (male, female) assigned at birth.

    MAIN OUTCOMES AND MEASURES: Development of long COVID, measured using a self-reported symptom-based questionnaire and scoring guideline at the first study visit that occurred at least 6 months after infection. Propensity score matching was used to estimate risk ratios (RRs) and risk differences (95% CIs). The full model included demographic and clinical characteristics and social determinants of health, and the reduced model included only age, race, and ethnicity.

    RESULTS: Among 12 276 participants who had experienced SARS-CoV-2 infection (8969 [73%] female; mean [SD] age at infection, 46 [15] years), female sex was associated with higher risk of long COVID in the primary full (RR, 1.31; 95% CI, 1.06-1.62) and reduced (RR, 1.44; 95% CI, 1.17-1.77) models. This finding was observed across all age groups except 18 to 39 years (RR, 1.04; 95% CI, 0.72-1.49). Female sex was associated with significantly higher overall long COVID risk when the analysis was restricted to nonpregnant participants (RR, 1.50; 95%: CI, 1.27-1.77). Among participants aged 40 to 54 years, the risk ratio was 1.42 (95% CI, 0.99-2.03) in menopausal female participants and 1.45 (95% CI, 1.15-1.83) in nonmenopausal female participants compared with male participants.

    CONCLUSIONS AND RELEVANCE: In this prospective cohort study of the NIH RECOVER-Adult cohort, female sex was associated with an increased risk of long COVID compared with male sex, and this association was age, pregnancy, and menopausal status dependent. These findings highlight the need to identify biological mechanisms contributing to sex specificity to facilitate risk stratification, targeted drug development, and improved management of long COVID.

    MeSH terms: Adult; Aged; Female; Humans; Male; Middle Aged; Prospective Studies; Risk Factors; Sex Factors; United States/epidemiology; Cohort Studies
  11. Swami V, Stieger S, Voracek M, Aavik T, Abdollahpour Ranjbar H, Adebayo SO, et al.
    PLoS One, 2025;20(1):e0313107.
    PMID: 39841629 DOI: 10.1371/journal.pone.0313107
    The Satisfaction With Life Scale (SWLS) is a widely used self-report measure of subjective well-being, but studies of its measurement invariance across a large number of nations remain limited. Here, we utilised the Body Image in Nature (BINS) dataset-with data collected between 2020 and 2022 -to assess measurement invariance of the SWLS across 65 nations, 40 languages, gender identities, and age groups (N = 56,968). All participants completed the SWLS under largely uniform conditions. Multi-group confirmatory factor analysis indicated that configural and metric invariance was upheld across all nations, languages, gender identities, and age groups, suggesting that the unidimensional SWLS model has universal applicability. Full scalar invariance was achieved across gender identities and age groups. Based on alignment optimisation methods, partial scalar invariance was achieved across all but three national groups and across all languages represented in the BINS. There were large differences in latent SWLS means across nations and languages, but negligible-to-small differences across gender identities and age groups. Across nations, greater life satisfaction was significantly associated with greater financial security and being in a committed relationship or married. The results of this study suggest that the SWLS largely assesses a common unidimensional construct of life satisfaction irrespective of respondent characteristics (i.e., national group, gender identities, and age group) or survey presentation (i.e., survey language). This has important implications for the assessment of life satisfaction across nations and provides information that will be useful for practitioners aiming to promote subjective well-being internationally.
    MeSH terms: Adolescent; Adult; Age Factors; Aged; Factor Analysis, Statistical; Female; Gender Identity*; Humans; Language; Male; Middle Aged; Personal Satisfaction*; Psychometrics/methods; Quality of Life; Surveys and Questionnaires; Young Adult; Self Report
  12. Elnaem MH, Bukhori NAS, Tengku Mohd Kamil TK, Rahayu S, Ramatillah DL, Elrggal ME
    Psychol Health Med, 2025 Mar;30(3):555-571.
    PMID: 39841970 DOI: 10.1080/13548506.2025.2450545
    Patients with type 2 diabetes mellitus (T2DM) are susceptible to mental health issues, impacting medication adherence and diabetes control. This study aimed to evaluate factors associated with depression and anxiety among T2DM patients in Indonesia and Malaysia. A cross-sectional study was conducted in Indonesia and Malaysia from October 2022 to April 2023 among T2DM patients. The study utilised an instrument with patient and disease data and three validated tools to assess depression, anxiety, and medication adherence. Statistical analysis, including binary logistic regression, was performed using SPSS® version 28 software. A study of 606 T2DM patients revealed that 56.5% were at risk of depression, while 41.6% were at risk of anxiety. Older patients with T2DM had lower rates of depression (AOR = 0.41, 0.25-0.68) and anxiety than younger patients. Normal-weight patients were less likely to experience depression and anxiety (AOR = 0.44, 0.27-0.72) than overweight patients. Patients without diabetic foot ulcers had a lower risk of depression (AOR = 0.34, 0.21-0.55) and anxiety than those with foot ulcers. Patients with a shorter duration of diabetes had a higher risk of depression (AOR = 3.27, 1.70-6.30) and anxiety than those with a longer duration. Patients on insulin-based regimens had higher rates of depression and anxiety (AOR = 2.28, 1.20-4.30) than those on metformin-based regimens. Nonadherent patients were more likely to experience depression and anxiety (AOR = 4.30, 2.22-8.32) than patients who adhered to their medication. The prevalence of depression and anxiety is concerning and influenced by factors such as age, diabetes duration, the presence of diabetic foot ulcers, and the prescribed medication regimen. Further efforts are necessary to enhance the mental health of T2DM patients and improve management outcomes.
    MeSH terms: Adult; Age Factors; Aged; Cross-Sectional Studies; Female; Humans; Hypoglycemic Agents/therapeutic use; Indonesia/epidemiology; Malaysia/epidemiology; Male; Middle Aged; Time Factors; Diabetic Foot/epidemiology; Diabetic Foot/psychology
  13. Tukimin SN, Karman SB, Wan Kamarul Zaman WS, Mohd Yunos NB, Syed Nor SN, Ahmad MY
    PMID: 39842129 DOI: 10.1016/j.saa.2024.125503
    Light-matter interaction has been profoundly studied for sample material classification. However, the optical classification of the sample through the polarized light-matter interaction remains underexplored. It is limited to the measurement of intensity instead of the angle of polarized light (AOP) for its degree of polarization. Measurement of the degree of polarization within a material or a medium becomes easier with a simple, low-cost and direct measurement without the need of any probing or labelling agent. Thus, this investigation was conducted mainly to determine the angle of polarized light (AOP) property of the crosslinked polymer using our proposed polarization measurement technique as an alternative approach of the material classification. The angle of polarized light (AOP) of each polymer was determined in combination property of polarization by absorption, transmission, and scattering. Our proposed scattered angle (ס=90°, 100°, 110°, and 120°) successfully measured the AOP of each polymer that can be classified into two groups. Group 1 represents the AOP value ( [Formula: see text] ) for a test sample of t1 = 3.1 %, 3.2, and 3.3 % with comparison to the normal sample (n = 3.0 %) and Group 2 represents the AOP value ( [Formula: see text] ) for the test sample oft2 = 3.4 %, 3.6 % and 3.7 % with comparison to the normal sample (n = 3.0 %). Our study proved a direct, easy, and simple method of determining the degree of polarization of the polymers without the need of complex formulation and labelling protocol. Therefore, this work may enhance the investigation of the optical properties of the agarose-based tissue-mimicking phantom (AGTMP) for modeling or simulation of the real biological sample in the future. Our polarization measures are worthy of further explored and implemented in current optical imaging techniques or sensing platform for optical classification of the biomaterials.
  14. Musa SM, Sining S, Arba'in AI, Amizuri MI, Lazim AM, Ghaffar MA
    Mar Pollut Bull, 2025 Jan 21;212:117584.
    PMID: 39842134 DOI: 10.1016/j.marpolbul.2025.117584
    Casting a shadow upon marine ecosystems worldwide, microplastic pollution emerges as a formidable threat, yet its repercussions on commercially significant marine fish remain largely unexplored. This investigation delves into the realm of microplastic contamination within three tuna species (Euthynnus affinis, Thunnus tonggol, and Auxis rochei) inhabiting Malaysian waters, with a keen focus on the gastrointestinal tract (GIT), gills, and edible muscle. These species were chosen due to their significant ecological roles and considerable commercial value, being extensively fished and widely consumed across global regions. Scrutinising 125 tuna specimens uncovers a spectrum of contamination levels across species, with T. tonggol registering the highest concentration (1.22 ± 0.14 microplastics per gram). Remarkably, male tunas exhibit a discernible proclivity towards heightened microplastic accumulation relative to their female counterparts. Noteworthy morphotypes of microplastics predominantly encompass fibres (92.43 %), notably manifesting in black hues (63.25 %). Notably, microplastics spanning the 0.01-1.00 mm size range assert their dominance, constituting a substantial 61.54 % of the total, with rayon emerging as the leading polymer (54.39 %). These findings highlight the pivotal role of the GIT and gills as primary sites of microplastic accumulation in tuna, raising concerns about potential human health implications through seafood consumption. It is crucial to deepen our understanding of microplastic distribution and characteristics in seafood to develop effective mitigation strategies and safeguard human well-being within marine environments. This study advocates for urgent global attention to address the ecological and human health impacts of microplastic contamination in marine ecosystems.
  15. Sulaiman IM, Bulgiba A, Kareem SA, Latip AA
    Methods Inf Med, 2025 Feb 11.
    PMID: 39842453 DOI: 10.1055/a-2521-4372
    OBJECTIVE:  This is the first Malaysian machine learning model to detect and disambiguate abbreviations in clinical notes. The model has been designed to be incorporated into MyHarmony, a natural language processing system, that extracts clinical information for health care management. The model utilizes word embedding to ensure feasibility of use, not in real-time but for secondary analysis, within the constraints of low-resource settings.

    METHODS:  A Malaysian clinical embedding, based on Word2Vec model, was developed using 29,895 electronic discharge summaries. The embedding was compared against conventional rule-based and FastText embedding on two tasks: abbreviation detection and abbreviation disambiguation. Machine learning classifiers were applied to assess performance.

    RESULTS:  The Malaysian clinical word embedding contained 7 million word tokens, 24,352 unique vocabularies, and 100 dimensions. For abbreviation detection, the Decision Tree classifier augmented with the Malaysian clinical embedding showed the best performance (F-score of 0.9519). For abbreviation disambiguation, the classifier with the Malaysian clinical embedding had the best performance for most of the abbreviations (F-score of 0.9903).

    CONCLUSION:  Despite having a smaller vocabulary and dimension, our local clinical word embedding performed better than the larger nonclinical FastText embedding. Word embedding with simple machine learning algorithms can decipher abbreviations well. It also requires lower computational resources and is suitable for implementation in low-resource settings such as Malaysia. The integration of this model into MyHarmony will improve recognition of clinical terms, thus improving the information generated for monitoring Malaysian health care services and policymaking.

  16. Alby A, Power A, Flaherty GT
    Travel Med Infect Dis, 2025 Jan 20.
    PMID: 39842532 DOI: 10.1016/j.tmaid.2025.102803
    INTRODUCTION: There has been limited literature reporting the nature of the consular assistance provided to travellers. This study aimed to describe the consular assistance activity of the Irish Department of Foreign Affairs.

    METHODS: Publicly available consular assistance data (2014-2023) were analysed descriptively. Consular missions were listed under 16-19 categories.

    RESULTS: A total of 26,378 consular assistance missions were recorded. The greatest proportion of missions related to COVID-19 repatriations (26.9%, n=7,097), deaths overseas (10%, n=2,650), and medical illness/mental health emergency abroad (11.6%, n=3,047). Forensic incidents (arrests/victims of crime) accounted for 12.9% (n=3,392) of consular assistance episodes.

    CONCLUSION: This study highlights the characteristics and trends of consular assistance requests received by a national governmental agency. Future studies should explore traveller awareness of consular procedures and the extent to which they are addressed in travel medicine consultations.

  17. Bushra SMR, Safuan S, Abu Bakar R, Lima MA, Abubakar BD, Nurul AA
    Int J Biol Macromol, 2025 Apr;299:140103.
    PMID: 39842582 DOI: 10.1016/j.ijbiomac.2025.140103
    Asthma is a chronic respiratory disease characterized by airway inflammation. Lignosus rhinocerotis (LR), a medicinal mushroom rich in polysaccharide, has been traditionally used to treat various diseases, including asthma. This study aimed to fractionate, characterize and evaluate the anti-asthmatic effects of polysaccharides from LR (LRP). LRP was isolated and characterized using high-performance liquid chromatography (HPLC), x-ray diffraction analysis (XRD), fourier transform infrared spectrometry (FTIR) and nuclear magnetic resonance (NMR) spectroscopy. In an OVA-induced asthma model, BALB/c mice were sensitized, challenged, and intranasally treated with LRP. Experimental findings demonstrated that LRP exhibits a semi-crystalline nature with predicted structure of → 4)-α-D-Glcp-(1 → and → 3)-β-D-Glcp-(1→. LRP significantly reduced eosinophilia, Th2 cytokines, and IgE levels. Histological analysis revealed LRP's ability to decrease epithelial damage and epithelial and smooth muscle thickness in lung. Reductions in inflammatory cell infiltration, mucus production, and transforming growth factor (TGF)-β1 expression were observed, although not statistically significant. Gene expression analysis indicated that LRP significantly downregulated the inducible nitric oxide synthase (iNOS) expression. This study highlights a detailed structural analysis of LRP and its potential as an alternative for the management of asthma. Further research is needed to elucidate the precise mechanisms of action and optimize its therapeutic application.
    MeSH terms: Animals; Disease Models, Animal; Female; Immunoglobulin E/blood; Lung/drug effects; Lung/pathology; Mice, Inbred BALB C*; Polysaccharides/pharmacology; Polysaccharides/chemistry; Cytokines/metabolism; Mice; Nitric Oxide Synthase Type II/metabolism
  18. Mohammad Azmi N, Nadzira A, Abdul Rahman NA, Mohd Azman ZA, Chandrakanthan SB, Dualim DM, et al.
    World J Surg, 2025 Jan 22.
    PMID: 39843355 DOI: 10.1002/wjs.12490
    BACKGROUND: Aims conventional bowel preparation restricts dietary intake up to 72 h prior to colonoscopy. Bowel preparation process is often perceived as unpleasant leading to poor compliance and subsequent poor bowel preparation. The aim of this trial is to compare the efficacy of low-residue semi-elemental enteral formula (LREF) incorporated diet versus the standard diet in polyethylene glycol (PEG)-based bowel preparation in the aim of creating a more tolerable bowel preparation regimen without compromising bowel cleanliness.

    METHODS: This was a multicenter, prospective, single-blinded, randomized controlled noninferiority trial. The noninferiority margin was set at 15%. One hundred sixty-seven patients were recruited and randomized to either the LREF group or the standard diet (SD) group using a 3L PEG preparation regimen.

    RESULTS: The LREF group results in comparable satisfactory preparation rating to the standard diet group with a mean BPPS score of 6.87 (SD 1.59) versus 7.14 (SD 1.54) (95% CI[-0.86; 0.32] and p = 0.367). The mean difference (MD) of the BBPS total score between the two groups was -0.27 (95% CI [-0.764 and 0.224]). Equivalence were demonstrated using the two one-sided test (alpha = 5%) with the lower t-value of 2.682 (p = 0.0042) and the upper t-value of -4.493 (p 

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