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Multiplexed automated digital quantification of fusion transcripts: comparative study with fluorescent in-situ hybridization (FISH) technique in acute leukemia patients

Akhter A 1 , Mughal MK 1 , Elyamany G 1 , Sinclair G 1 , Azma RZ 2 , Masir N 2 Show all authors , Shuib S 2 , Rashid-Kolvear F 1 , Shabani-Rad MT 1 , Stewart DA 3 , Mansoor A 4

Affiliations 

  • 1 Department of Pathology and Laboratory Medicine, University of Calgary and Calgary Laboratory Services, Calgary, AB, Canada
  • 2 Department of Pathology, University Kebangsaan Malaysia, Kuala Lumpur, Cheras, Malaysia
  • 3 Departments of Oncology and Medicine, University of Calgary, Calgary, AB, Canada
  • 4 Department of Pathology and Laboratory Medicine, University of Calgary and Calgary Laboratory Services, Calgary, AB, Canada. Adnan.Mansoor@cls.ab.ca
Diagn Pathol, 2016 Sep 15;11(1):89.
PMID: 27632978 DOI: 10.1186/s13000-016-0541-z

Abstract

The World Health Organization (WHO) classification system defines recurrent chromosomal translocations as the sole diagnostic and prognostic criteria for acute leukemia (AL). These fusion transcripts are pivotal in the pathogenesis of AL. Clinical laboratories universally employ conventional karyotype/FISH to detect these chromosomal translocations, which is complex, labour intensive and lacks multiplexing capacity. Hence, it is imperative to explore and evaluate some newer automated, cost-efficient multiplexed technologies to accommodate the expanding genetic landscape in AL.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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