Displaying publications 961 - 980 of 4000 in total

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  1. Noor Ifatul, H.M.D., Lee, H.Y., Mahyudin, N.A., Nazamid, S., Wan Norhana, M.N.
    MyJurnal
    Marine fungi are potential source of bioactive compounds as indicated by the increasing statistic
    of research findings. However similar research in Malaysia is still lacking. Hence, this study
    is undertaken to determine the antibacterial activity of four marine fungal isolates (PR1T4,
    PP2L4, PR3T13 and PR5T4) from Pulau Redang and Pulau Payar Marine Parks, Malaysia
    against Salmonella Typhi, Listeria monocytogenes, Staphylococcus aureus, and Escherichia
    coli. Fungal isolates were first macroscopically and microscopically characterized and later
    molecularly identified as Penicillium citrinum, Sacroladium strictum, Aspergillus sydowii and
    Aspergillus sp. respectively. Solid and broth fermentation of fungi were carried out to produce
    crude extracts and these extracts were screened for antibacterial activity. In general, solid
    fermentation extracts (SFE) showed significantly higher antibacterial activity (p
  2. Ang Pei-Shen, Rajesh Ramasamy, Noor Hamidah Hussin, Cheong Soon-Keng, Seow Heng-Fong, Maha Abdullah
    MyJurnal
    Introduction: The phenotype and genotype of cancer cells portray hallmarks of cancer which may
    have clinical value. Cancer cell lines are ideal models to study and confirm these characteristics. We
    previously established two subtracted cDNA libraries with differentially expressed genes from an
    acute myeloid leukaemia patient with poor prognosis (PP) and good prognosis (GP). Objective: To
    compare gene expression of the leukaemia associated genes with selected biological characteristics
    in leukaemia cell lines and normal controls. Methodology: Expression of 28 PP genes associated
    with early fetal/embryonic development, HOX-related genes, hematopoiesis and aerobic glycolysis/
    hypoxia genes and 36 GP genes involved in oxidative phosphorylation, protein synthesis, chromatin
    remodelling and cell motility were examined in B-lymphoid (BV173, Reh and RS4;11) and myeloid
    (HL-60, K562) leukaemia cell lines after 72h in culture as well as peripheral blood mononuclear cells
    from healthy controls (N=5) using semi-quantitative polymerase chain reaction (PCR) method. Cell
    cycle profiles were analysed on flow cytometry while MTT cytotoxicity assay was used to determine
    drug resistance to epirubicin. Results: Genes expressed significantly higher in B-lymphoid leukaemia
    cell lines compared to healthy controls were mostly of the GP library i.e. oxidative phosphorylation
    (3/10), protein synthesis (4/11), chromatin remodelling (3/3) and actin cytoskeleton genes (1/5). Only
    two genes with significant difference were from the PP library. Cancer associated genes, HSPA9 and
    PSPH (GP library) and BCAP31 (PP library) were significantly higher in the B-lymphoid leukemia cell
    lines. No significant difference was observed between myeloid cell lines and healthy controls. This
    may also be due heterogeneity of cell lines studied. PBMC from healthy controls were not in cell cycle.
    G2/M profiles and growth curves showed B-lymphoid cells just reaching plateau after 72 hour culture
    while myeloid cells were declining. IC50 values from cytotoxicity assay revealed myeloid cell lines had
    an average 13-fold higher drug resistance to epirubicin compared to B-lymphoid cell lines. Only CCL1,
    was expressed at least two-fold higher in myeloid compared to B-lymphoid cell lines. In contrast,
    MTRNR2, EEF1A1, PTMA, HLA-DR, C6orf115, PBX3, ENPP4, SELL, and IL3Ra were expressed
    more than 2-fold higher in B-lymphoid compared to myeloid cell lines studied here. Conclusion: Thus,
    B-lymphoid leukaemia cell lines here exhibited active, proliferating characteristics closer to GP genes.
    Higher expression of several genes in B-lymphoid compared to myeloid leukaemia cell lines may be
    useful markers to study biological differences including drug resistance between lineages.
  3. Valerie Anak MIichael, Khairul Aidil Azlin Abdul Rahman, Shureen Faris Abdul Shukor, Noor Azizi Mohd Ali
    MyJurnal
    Living in today's era, artists’ practice in creating artwork is different from those practiced 20 years ago. With the existence of digital art followed by electronic art in the local art scene, the presentation, style and approach have evolved. Back in 2005 and 2007, some exhibitions brought hybrid art concept into an art exhibition. One of the shows was ‘Hybrid+ism’ which highlighted in various disciplines, media, applying unlimited processes and medium. The purpose of this paper is to study the artists’ practice in the hybrid art based on selected artworks. The artworks are selected based on some criteria ranging from experimental, mixed media, multi-disciplines, integration and technology as well as culture aspects. This paper is discussing the hybrid art practice among artists in the Malaysian art scene and the challenges to creating awareness on hybrid practice among audiences.
  4. Noor Faradila, P., Mohd Syazwan, S., Azhar, H., Mohd Rasid, O., Baba, M.D.
    MyJurnal
    Child restraint system (CRS) can protect children in the event of crash and reduce the severity of injuries. As such, it
    is crucial to understand the prevalence of CRS usage and knowledge attributes on CRS usage among drivers. This
    study aims to assessdrivers’ knowledge on CRS usage.A semi-structured questionnaire was developed and pilot tested
    to verify its validity and reliability. The questionnaireaddressesdrivers’ knowledge about CRS currently available in
    the Malaysian market, the types of restraints used and views on fitting restraints to passenger vehicles. Seventy four
    percentfrom 500 respondents cited that they are usingor theyhave used CRS,64% knowabout CRS and its function, and
    43% areawareof ISOFIX. In short, majority of the respondents are aware of CRS use and functions. Awareness and
    education program should berigorously introduced to public towards the implementation of the CRS law.
  5. Wan Hasrulnizzam, W.M., Fatimah, A., Seri Rahayu, K., Ruzy Haryati, H., Noor Amira, M.R.
    MyJurnal
    Research on environmental ergonomics of train/commuter is very limited. Thus, this study was conducted with aims
    to determine the environmental ergonomic of public transport, whether it is in accordance to indoor air quality
    standard inside women coach cabin train during operations. Although the number of passengers is increasing, some
    claimed that the indoor air quality for the Malaysian commuter train is uncertain, especially at peak times.
    Unsatisfactory feedback from some respondents—especially female passengers—will affect other passengers to
    remain loyal to use this facility as one of the main transportation to reduce the traffic congestion that will be faced
    if using private vehicles. The study conducted during peak hours on the weekends and the trip took exactly two hours
    and covered 18 main stations. The measurement devices placed at the centre of the cabin Komuter using Air Quality
    Meter (AIRFLOW Instrument Model TA465). The main parameters measured were temperature, humidity, and carbon
    dioxide (CO2). This study also counts the number of passengers riding the train. The results showed that the indoor
    air quality (AIQ) level of the morning hour will affect passengers’ health more compared to the evening hour (this is
    due to the CO2 level that has exceeded the safety standard). Morning hour gave higher reading of CO2 (43.8%) and
    relative humidity (17%) compared to evening hour. Evening hours also showed greater temperature at 3%. The
    findings can give awareness to the train company to improve the IAQ by installing a suitable ventilation system and
    can give comfort to the passengers when travelling using Malaysian commuter train.
  6. Mohd Izuan Effendi Halmi, Wan Lutfi Wan Johari, Mohd Shukuri Mohd Ali, Noor Azmi Shaharuddin
    MyJurnal
    Molybdenum is reported to be very toxic to ruminants and shows evidence of spermatogenesis
    toxicity in animals and insects. Hence, its removal is important. In this study, we report on the
    first isolation of molybdenum-reducing bacterium from agricultural soil. The bacterium reduces
    hexavalent molybdenum (sodium molybdate) to molybdenum blue (Mo-blue); a colloidal
    product, which can be trapped and removed from solution. Phylogenetic analysis resulted in a
    tentative identification of the bacterium as Serratia sp. strain MIE2. The optimum conditions for
    Mo-blue production using the normal one-variable-at-a-time (OVAT) approach were 10 mM of
    sodium molybdate, pH 6.0, a temperature of 35°C, ammonium sulphate at 10 g/L as the nitrogen
    source and sucrose concentrations of between 30-50 g/L as the carbon source and electron donor
    for molybdate. Studies on the effects of pesticides and solvents on Mo-blue production showed
    that Mo-blue production from whole cells was relatively more affected by these xenobiotics
    compared to the crude enzyme. Nevertheless, the strain was resistant to most of the xenobiotics
    tested. Based on the strain MIE characteristics, the bacterium will be a suitable candidate for the
    remediation of aquatic bodies and agricultural soils contaminated with molybdenum.
  7. Rodiah, M.H., Nur Asma Fhadhila, Z., Noor Asiah, H., Aziah, M.Y., Kawasaki, N.
    MyJurnal
    This study investigated the use of ultrasound-assisted extraction (UAE) and agitated-bed extraction (ABE) to improve extraction efficiency of dyes from exocarp and mesocarp of Cocos nucifera. The UAE method was carried out at a frequency of 37 kHz and power of 150 W at 30°C for 1 hour and ABE technique was performed at 30°C for 24 hours at the agitation of 150 rpm. The effects of different solvent types (methanol, ethanol and acetone) and the different percentage of solvent (20%-70%) were determined. The results showed although the colourant yield increased with the increase of solvent concentration up to 60%, statistic value indicated no significant difference (p > 0.05) for the value of yield using solvent range from 20% to 70% for both UAE and ABE methods. Therefore, the solvent concentration of 20% was selected as the optimum concentration for each type of solvent used for both ABE and UAE methods. In comparison, higher extraction yields were achieved by UAE method where the optimal yield of the colourant of the mesocarp was 7.6% using acetone as solvent and the exocarp yielded about 6.4% using acetone or methanol. The recovery of natural colourant using ultrasound was found to be highly dependent on the type of solvents (acetone > methanol > ethanol) for both mesocarp and exocarp. The present study suggests that UAE method should be employed for hauling out colouring materials from exocarp and mesocarp due to its effectiveness in terms of time and economical usage of solvent.
  8. Nor Farid Mohd Noor, Mohamad Ezany Yusoff, Suharni Mohamad, Ahmad Mukifza Harun, Shahril Yusof
    MyJurnal
    Staphylococcus aureus (S. aureus) is an important bacterium with significant pathological implications
    in the field of medicine. Attempting to cure bacterial infections at an advanced stage results in considerable waste
    of time, effort and expenditure. Thus, the prevention of such illnesses is paramount. Besides using chemical
    drugs to treat infections, several non-organic extracts have been tested in trials and been shown to impede the
    bacteria’s growth. This paper proposes that the modified hydrothermal nanotitania extract has great potential to
    combat this lethal organism. The viability of S. aureus was shown to be markedly reduced following the addition
    of nanotitania extract with 0.01%, 0.03% and 0.05% silver after 24, 48, and 72 hours. The ability of the
    nanotitania extract to inhibit the growth of S. aureus indicates its antimicrobial characteristics.
  9. Nurbaity Sabri, Noor Hazira Yusof, Zaidah Ibrahim, Zolidah Kasiran, Nur Nabilah Abu Mangshor
    Scientific Research Journal, 2017;14(2):49-62.
    MyJurnal
    Text localisation determines the location of the text in an image. This process
    is performed prior to text recognition. Localising text on shop signage is
    a challenging task since the images of the shop signage consist of complex
    background, and the text occurs in various font types, sizes, and colours.
    Two popular texture features that have been applied to localise text in
    scene images are a histogram of oriented gradient (HOG) and speeded up
    robust features (SURF). A comparative study is conducted in this paper
    to determine which is better with support vector machine (SVM) classifier.
    The performance of SVM is influenced by its kernel function and another
    comparative study is conducted to identify the best kernel function. The
    experiments have been conducted using primary data collected by the
    authors. Results indicate that HOG with quadratic kernel function localises
    text for shop signage better than SURF.
  10. Ramanoon, S.Z., Sadiq, M.B., Noor Maisara Abdul Razak, Mansor, R., Syed-Hussain, S.S., Shaik Mossadeq, W.M.
    Jurnal Veterinar Malaysia, 2018;30(1):1-6.
    MyJurnal
    This study was designed to characterise the lameness cases in cattle reported to the University Veterinary Hospital (UVH) of
    Universiti Putra Malaysia (UPM), Serdang, Selangor. Medical records from 2013 to 2017 were reviewed and 136 cases of cattle
    diagnosed as lameness were retrieved. Information concerning signalment, location of lesions, and cause of lameness was analysed.
    Majority of the cases were reported in dairy (77%) compared with beef cattle (23%) with 73% being under semi-intensive
    management system. Foot lesions accounted for about 51% of cases, while lesions were more on the hindlimbs than the forelimbs
    (p0.05) between foot
    lesions categorised either as infectious or non-infectious causes. A higher proportion (p
  11. Cheam, Chai Li, Nurul Amanina Othman, Faten Noor Saadah Ahmad Khir Johari
    MyJurnal
    The aim of this paper is to determine tourists’ perceptions on spiritual experience, brand image,
    cultural attributes and service quality they experienced in Kota Bharu, the capital and administration
    centre of Kelantan, Malaysia. Kota Bharu was declared an Islamic City in 2005. It has many mosques,
    various museums, unique architectural old buildings and palaces. As the number of tourist arrivals to
    Kelantan is encouraging and contributing to the state income; it is important to examine what these
    tourists perceive during their stays in Kota Bharu Islamic City. Variance Based Structural Equation
    Modelling technique of Partial Least Square (PLS) was employed. The findings show that all the
    variables are significantly influencing tourists’ perception except for service quality. In other words,
    tourists’ perceptions of Kota Bharu are positively related to spirituality, brand destination and cultural
    attributes. Tourists’ perceptions play a crucial role as this will affect the future tourist arrivals in the
    state. Thus, the findings of the study will be useful in helping policy planners to understand how
    tourists perceived Kota Bharu and design policies to increase tourism receipts and to preserve tourism
    attributes.
  12. Fadzil, M.S.A., Mohd Noor, N., Tamchek, N., Ung, N.M., Alawiah, A., Abdullah, N., et al.
    MyJurnal
    The thermoluminescence kinetic parameters of Ge-doped flat fibre have been investigated comprehensively using the computerized glow curve deconvolution analysis. The Ge-doped flat fibre was irradiated to 6 MV and 10 MV photon beam with dose ranging from 100 cGy to 300 cGy. Analysis was done using WinGCF software on the dependence of the glow curve intensity on dose delivered and the determination of the trap parameters. Ge-doped flat fibre was found to be linear over the entire dose range explored for both 6 MV and 10 MV irradiations with r2 value of 0.9955 and 0.9933 respectively. The glow curve consists of five individual glow peaks. The peak height increases with increasing irradiation dose. The first peak (P1) is a dominant individual peak for low temperature peak (LTP) with the maximum temperature ranging from 167.4°C to 179.0°C for both beams studied. Meanwhile, peak (P4) is a dominant individual glow curve for high temperature peak (HTP) with maximum temperature for 6 MV and 10 MV and is observed between 264.5°C to 279.4°C. Peak 1 has the lowest activation energy which is less than 0.72 eV while peak 2 shows the highest activation energy (1.3 eV < Ea < 2.1 eV) which indicates the deepest electrons trap. The results showed that the peak integral will increases as the dose increases. The Ge-doped flat fibre demonstrated the second-order kinetic peak behavior by exhibiting the symmetric shape of the glow curve with high temperature half of the curve slightly broader than the low temperature half, which suggests the possibility of electron retrapping.
  13. Noor Hilmi Mohd Johan, Mohd Firdauz Mohd Fathir, Nurul Aishah Ab Raman
    MyJurnal
    The changing of geopolitical environment in Northeast Asia after the Cold War illustrates some
    fundamental shift in Japan’s security environment. The post-Cold War era provide a new set of
    challenges and difficulties that must be diligently comprehend not only by Japanese policy maker but
    also her military echelon as well. With the receding of Soviet Union threat from the north comes a
    new and emerging volatile situation from her immediate neighbors; People’s Republic of China and
    Democratic Republic of Korea. Aside from this, threats from Japan domestic security also did not
    demonstrate any indication of declination. This article explores each international actor that can be
    considered as security predicament to Japan and why each actor behaves in an aggressive manner
    towards Japan. By analyzing issues that occur both domestic and beyond her border, the author show
    how past threats that emerged will eventually be replace by a new and more volatile situations –
    instances where PRC challenging Japan sovereignty over Senkaku and DPRK nuclear and ballistic
    missile test. Variety of methodology tools has been used to provide an adequate data analysis to
    interpret every states action in Northeast Asia, ranging from the using of primary and secondary
    sources that seek to contribute to the argument of this article.
  14. Wan Maisarah Mukhtar, Alia Athira Tarmuji, Noor Syuhaida Mohamed, Muhammad Syahir Jafree
    MyJurnal
    With the increasing demand for greater quality of transferred data, the optical cable
    lines are reaching their limits of transfer capacities. The alternative for more effective
    usage is by introducing the Dense Wavelength Division Multiplexing (DWDM)
    integrated with optical amplifier to optimize the output signal. This study was
    performed theoretically with an assistance of OptiSystem 9.0 simulation software to
    develop higher transfer speed of 32 channels DWDM network system by employing
    hybrid optical amplifiers. Three types of optical amplifiers had been introduced such
    as Erbium Doped Fiber Amplifier (EDFA), Semiconductor Optical Amplifier (SOA) and
    Dynamic RAMAN amplifier. The optimum performance of DWDM system was obtained
    by employed hybrid EDFA-Raman amplifier which resulted the best transmission signal
    received with maximum Qfactor=43.0579 a.u. The hybrid EDFA-Raman produced
    better stability than EDFA-SOA where the received signals were only fluctuated within
    ±3.73 a.u. In comparison with other types of configuration, namely EDFA-SOA and
    RAMAN-SOA; the value of maximum Q-factor experienced about 50% of increment. In
    conclusion, the development of high performance and excellent stability of 32-
    channels DWDM optical network system can be achieved by introduced hybrid
    amplifier of EDFA-RAMAN.
  15. Das, Priscilla, Naing, NyiNyi, Nadiah Wan-Arfah, Naing Noor Jan, K.O., Yee, Cheng Kueh, Rasalingam, Kantha
    JUMMEC, 2019;22(1):50-57.
    MyJurnal
    Background: This paper investigates the quality of life of brain pathology patients in relation to their sociodemographic
    profiles and clinical factors.

    Methods: This is a cross-sectional study done at a tertiary referral hospital in Kuala Lumpur. A total of 100
    patients were recruited in the study after excluding 22 patients who did not met the exclusion criteria. The
    European Organisation for Research and Treatment of Cancer Quality of Life (EORTC QLQ-C30) self-administered
    questionnaire was utilized in the study. The Global health status/QoL, Physical functioning, Role functioning,
    Emotional functioning, Cognitive functioning, Social functioning, Fatigue, Nausea and vomiting, Pain, Dyspnoea,
    Insomnia, Appetite loss, Constipation, Diarrhoea, and Financial difficulties were assessed in this study.

    Results: The most severe impairment in functioning was with lowest score of cognitive functioning (mean
    score=61) and the most severe symptom was fatigue (mean score=45). There were significant differences in
    quality of life scores in different socio-demographic groupsand types of brain pathology patients. Patients aged
    below 40 years old or less had better physical functioning, less symptoms of fatigue and insomnia compared
    to patients who were more than 40 years old. Male patients faced more financial difficulties compared with
    female patients. Patients who were married had increased insomnia compared to the single patients. Employed
    patients had better physical functioning and less financial difficulties compared with patients who were
    unemployed. Patients who earned >RM 2500.00 monthly had better physical functioning, less symptoms of pain and less financial difficulties than patients who earned ≤RM 2500.00. Patients with qualifications lower
    than SPM tended to face more financial difficulties compared to patients with qualifications of SPM or higher.
    Meningioma patients had better social functioning compared with others, whereas Carvenoma patients had
    better physical functioning. Meningioma patients had more symptoms of insomnia compared with other
    patients. All the findings were with p value less than 0.05.

    Conclusion: The quality of life of patients with brain pathology is affected by socio-demographic factors and
    clinical diagnoses. Efforts should be made to improve the overall quality of life of these patients.
  16. Noor Shaeda Ismail, Irniza Rasdi, Sarva Mangala Praveena, Emilia Zainal Abidini
    MyJurnal
    Calcium Carbide (CaC2) use for fruit ripening become dubious in recent years when various health related issues began to arise. This study aims to assess knowledge, attitude and practises (KAP) and health related to CaC2 among mango farmers, staff/workers working in mango farms and fruits traders in Perak, Kedah and Perlis. Methods: All potential respondents in the study locations was invited to participate. Yes-no questions were used to assess KAP while structured questions were used to obtain socio-demographic data through face-to face interview. Results: The response rate for this study was 67.8% (n = 200). All respondents were male with the average age of 40.45 years old. Majority of them (67.5%) completed secondary school as their highest education level. Overall, less than half of the total respondents have appropriate KAP. Only job tenure significantly associated with the knowledge on the type of suitable PPE (χ2 = 16.421). Respondents with secondary or tertiary school of education level (χ2= 9.154) and those with 1-5 years of job tenure (χ2 = 10.932) showed significantly better attitude on CaC2 handling. Also, the total scores of knowledge significantly associated with attitude (χ2 = 26.801) and application of glove (χ2= 12.551). In multiple logistic regression only respondents with the knowledge on type of PPE (OR = 6.30) will persist to use CaC2. Conclusion: The KAP related to CaC2 among respondents is low and their knowledge was significantly associated with their attitude and practice. Therefore, effective training and communication approach is warranted.
  17. Yao PP, Mohd Firdaus Raih, Hasidah Mohd Sidek, Noor Embi, Wan Kl
    Sains Malaysiana, 2016;45:1947-1957.
    Penemuan sasaran dadah antikoksidia baharu merupakan antara usaha yang diperlukan untuk mengawal penyakit koksidiosis ayam yang disebabkan oleh spesies Eimeria. Dalam kajian ini, serpihan yang mengekodkan glikogen sintase kinase-3 (GSK-3) Eimeria tenella putatif telah diamplifikasi daripada cDNA E. tenella. Hasil pemadanan homologi menunjukkan jujukan GSK-3 E. tenella yang terjana mempunyai padanan yang tinggi dengan jujukan GSK-3 organisma lain. Domain terpulihara GSK-3 dan residu yang penting untuk aktiviti GSK-3 juga diramalkan hadir dalam jujukan GSK-3 E. tenella. Analisis struktur sekunder serta pemodelan homologi menunjukkan pembahagian struktur protein kepada domain bebenang beta pada hujung N dan domain heliks alfa pada hujung C, yang merupakan ciri enzim GSK-3. Kesemua hasil analisis ini menyokong bahawa jujukan yang dikaji mengekodkan protein GSK-3 dalam E. tenella. Walaupun darjah keterpuliharaan adalah tinggi, namun terdapat perbezaan yang bermakna diperhatikan antara GSK-3 E. tenella dan perumahnya. Residu Ser 9 yang dilaporkan penting untuk perencatan aktiviti GSK-3 didapati tidak terpulihara dalam GSK-3 E. tenella. Memandangkan Ser 9 merupakan tapak pemfosfatan bagi GSK-3β dalam haiwan vertebrata, ketiadaan residu ini dalam jujukan GSK-3 E. tenella mencadangkan bahawa pengawalaturan GSK-3 E. tenella melibatkan tapak pemfosfatan dan mekanisme yang berbeza. Tambahan pula, hasil analisis filogenetik menunjukkan bahawa GSK-3 E. tenella mempunyai pertalian yang rapat dengan protein GSK-3 tumbuh-tumbuhan. Analisis superposisi GSK-3 E. tenella dengan GSK-3β Homo sapiens pula menunjukkan bahawa perencat GSK-3 mampu berinteraksi dengan protein GSK-3 E. tenella. Keputusan kajian ini mencadangkan bahawa GSK-3 E. tenella mempunyai potensi untuk diperkembangkan sebagai sasaran dadah antikoksidia.
  18. Halimah Abdullah Sani, Nur Azanim Darus, Mahanem Mat Noor, Isma Illyani Ismail
    Diabetis mellitus merupakan sejenis penyakit metabolik yang disebabkan oleh peningkatan aras glukosa darah serta memberi kesan komplikasi terhadap kesihatan. Peningkatan aras glukosa dalam darah berpunca daripada kegagalan penghasilan insulin yang berperanan dalam mengawalatur aras glukosa darah. Lebih 90 % pesakit diabetis turut mengalami masalah gangguan fungsi seksual lelaki seperti penurunan libido, impoten dan infertiliti di mana kesemua masalah ini adalah disebabkan oleh kegagalan testis yang berkaitan dengan ciri hiperglisemia pesakit diabetis itu sendiri. Infertiliti merujuk kepada ketidaksuburan sperma atau ketidakupayaan individu untuk menghasilkan sperma yang normal. Kajian ini dijalankan untuk melihat potensi ekstrak akues Gynura procumbens (GP) untuk menurunkan aras glukosa darah serta meningkatkan kualiti sperma tikus jantan strain Sprague dawley teraruh diabetis. Tikus diaruh diabetik melalui suntikan intravena streptozotocin (STZ), 65 mg/kg. Kualiti sperma ditentukan berdasarkan parameter berbeza yang dikaji iaitu bilangan, motiliti dan peratus mortaliti sperma. Tikus-tikus ini dibahagi kepada dua kumpulan iaitu normal dan diabetik. Setiap kumpulan ini dibahagi kepada 2 sub kumpulan dengan perlakuan yang berbeza iaitu kawalan (n=5) dan dos 50 mg/kg (n=5) di mana tikus ini diberikan rawatan selama 10 hari berturut-turut menggunakan ekstrak akues GP melalui kaedah suap paksa. Hasil menunjukkan rawatan dengan ekstrak GP menurunkan aras glukosa darah tikus diabetik daripada 15.08 ± 1.878 mmol/L kepada 10.42 ± 2.71 mmol/L secara signifikan (p<0.05). Rawatan dengan ekstrak GP turut meningkatkan bilangan sperma normal dalam tikus diabetik secara signifikan (p<0.05). Bilangan sperma normal dalam tikus diabetik yang diberi rawatan ekstrak ialah (50.23 ± 3.12) x 107 berbanding tikus kawalan diabetik (39.93 ± 8.06) x 107. Sebanyak 38.5% tikus kawalan diabetik menghasilkan motiliti sperma gred d iaitu sperma tidak bergerak (immotil). Walau bagaimanapun, tikus diabetik yang diberi rawatan ekstrak GP menunjukkan penurunan sperma dalam gred d kepada 30.48% serta peningkatan sperma gred a iaitu sperma bergerak paling pantas daripada 31.25% kepada 34.86%. Tikus diabetik yang dirawat dengan ekstrak GP menunjukkan penurunan secara signifikan (p<0.05) dalam peratusan mortaliti sperma iaitu daripada 50.09 ± 14.34% kepada 30.57 ± 4.61%. Hasil ini menunjukkan ekstrak akues Gynura procumbens dapat menurunkan aras glukosa darah, merawat ketidaksuburan serta meningkatkan spermatogenesis pada tikus diabetik jantan.
  19. Shahrul Hisham Zainal Ariffin, Rahmah Mohamed, Zulkeflie Zamrod, Mohammad Noor Embi
    Bahagian aktif bagi enzim toksin bakteria daripada Burkholderia pseudomallei, Pseudomonas aeruginosa dan difteria merupakan domain ADP-ribosilasi. Domain ini didapati terpelihara di antara ketiga-tiga mikroorganisme. Di dalam kajian ini, domain ADP-ribosilasi Burkholderia pseudomallei telah diamplifikasi daripada genom B. pseudomallei virulen dengan menggunakan pencetus-pencetus yang dibina berdasarkan kepada jujukan domain ADP ribosilasi Pseudomonas aeruginosa. Hasil DNA amplifikasi ditulenkan dan digunakan sebagai prob (HPCR2) untuk menyaring DNA selitan daripada B. pseudomallei yang diklonkan ke dalam vektor pengekspresan pSport-I. Objektif kajian ini adalah untuk menyaring lapan klon yang positif hasil daripada penyaringan awal melalui pendekatan immunoblot menggunakan antitoksin daripada arnab. Penyaringan ini juga melibatkan tiga klon yang tidak memberikan isyarat positif semasa penyaringan secara immunoblot. Keputusan menunjukkan hanya satu klon (L31) daripada lapan klon immunoblot positif mempunyai domain ADP-ribosilasi. Penjujukan DNA separa klon L31 secara manual melibatkan dua pencetus menghasilkan jujukan sepanjang 450pb. Analisis selanjutnya mendapati daripada enam kemungkinan translasi kepada polipeptida hanya satu polipeptida wujud yang tidak mempunyai sebarang kodon penamat pada jujukan kodonnya.
  20. NORITA HASSAN, SAHRIM AHMAD, NORHAMIDI MUHAMAD, MOHD AFIAN OMAR, NOOR AZLINA HASSAN
    Sains Malaysiana, 2013;42:1787-1791.
    Moldability of metal injection molding (MIM) is dependent on the outward appearance of the resultant feedstock. Properties of the binders used will influence the properties of the feedstock. Stainless steel powder 316L with mean size 22 μm and the binder system consists of three major fractions of paraffin wax, thermoplastic natural rubber and stearic acid with a powder loading of 65 vol. % was investigated. Comparison was also made with existing palm stearin in the binder system replacing the paraffin wax. Kinetic solvent extractions were done to determine the differences between the binder systems. The feedstock was then injected into tensile bar using vertical injection machine. The results showed that there is a slightly time extension during the solvent extraction as a comparison. The feedstock has been successfully injection molded at 190-200°C. Study of thermal analysis such as DSC and TGA has been done as a preparation for the thermal debinding and sintering process. This study demonstrated that a backbone polymer; thermoplastic natural rubber performs best in term of flow stability and compact quality and also saves in processing time.
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