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Proteomic Investigations of In Vitro and In Vivo Models of Periodontal Disease

Binti Badlishah Sham NI, Lewin SD, Grant MM

Proteomics Clin Appl, 2020 05;14(3):e1900043.
PMID: 31419032 DOI: 10.1002/prca.201900043

Proteomics has currently been a developing field in periodontal diseases to obtain protein information of certain samples. Periodontal disease is an inflammatory disorder that attacks the teeth, connective tissues, and alveolar bone within the oral cavity. Proteomics information can provide proteins that are differentially expressed in diseased or healthy samples. This review provides insight into approaches researching single species, multi species, bacteria, non-human, and human models of periodontal disease for proteomics information. The approaches that have been taken include gel electrophoresis and qualitative and quantitative mass spectrometry. This review is carried out by extracting information about in vitro and in vivo studies of proteomics in models of periodontal diseases that have been carried out in the past two decades. The research has concentrated on a relatively small but well-known group of microorganisms. A wide range of models has been reviewed and conclusions across the breadth of these studies are presented in this review.

Matched MeSH terms: Mass Spectrometry
Fulltext Determination of polycyclic aromatic hydrocarbons in human blood samples using solid phase extraction and gas chromatography mass spectrometry- a review

Samer Al-Battawi, Yu Bin Ho, Mohd Talib Latif, Vivien How, Karuppiah Thilakavathy

Malaysian Journal of Medicine and Health Sciences, 2019;15(204):165-169.
MyJurnal

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants with toxic effects and adverse health impacts on general population. Several methods of extraction had been applied to extract PAHs from human blood samples such as solid phase extraction (SPE). The SPE represents one of the most common techniques for extraction and clean-up procedures as it needs low quantity of solvents with less manual efforts. Similarly, various analytical instruments like gas chromatography coupled to mass spectrometry (GC-MS) was used to measure the PAHs levels. Gas chromatog- raphy is a simple, fast, and very efficient method for solvents and small organic molecules. This review provides an overview of the measured concentrations of PAHs in human blood samples through the application of SPE and GC- MS during the last ten years. While these studies used various solvents, their application of SPE method and GC-MS revealed rewarding results about the determination of PAHs levels in the human samples.

Matched MeSH terms: Gas Chromatography-Mass Spectrometry
Qualitative and Quantitative Analysis of Mycotoxins

Rahmani A, Jinap S, Soleimany F

Compr Rev Food Sci Food Saf, 2009 Jul;8(3):202-251.
PMID: 33467794 DOI: 10.1111/j.1541-4337.2009.00079.x

Mycotoxin toxicity occurs at very low concentrations, therefore sensitive and reliable methods for their detection are required. Consequently, sampling and analysis of mycotoxins is of critical importance because failure to achieve a suitable verified analysis can lead to unacceptable consignments being accepted or satisfactory shipments unnecessarily rejected. The general mycotoxin analyses carried out in laboratories are still based on physicochemical methods, which are continually improved. Further research in mycotoxin analysis has been established in such techniques as screening methods with TLC, GC, HPLC, and LC-MS. In some areas of mycotoxin method development, immunoaffinity columns and multifunctional columns are good choices as cleanup methods. They are appropriate to displace conventional liquid-liquid partitioning or column chromatography cleanup. On the other hand, the need for rapid yes/no decisions for exported or imported products has led to a number of new screening methods, mainly, rapid and easy-to-use test kits based on immuno-analytical principles. In view of the fact that analytical methods for detecting mycotoxins have become more prevalent, sensitive, and specific, surveillance of foods for mycotoxin contamination has become more commonplace. Reliability of methods and well-defined performance characteristics are essential for method validation. This article covers some of the latest activities and progress in qualitative and quantitative mycotoxin analysis.

Matched MeSH terms: Tandem Mass Spectrometry
Fulltext Contribution of aroma compounds to the antioxidant properties of roasted white yam (Dioscorea rotundata)

Lasekan O, Teoh LS

BMC Chem, 2019 Dec;13(1):133.
PMID: 31891159 DOI: 10.1186/s13065-019-0650-3

Background: The aroma chemistry and the contribution of the aroma compounds to the anti-oxidative properties of roasted yam have yet to be characterized. The growing popularity of roasted yam in regions where they are being consumed calls for a concerted effort to elucidate their aroma chemistry as well as their anti-oxidative properties.
Results: The aroma compounds in roasted white yam (Dioscorea rotundata) were isolated and identified using static headspace-gas chromatography-mass spectrometry (SH-GC-MS) and gas chromatography-olfactometry (GC-O). In addition, the anti-oxidative activities of the most abundant volatile heterocyclic compounds (2 pyrroles, 4 furans and 3 pyrazines) were evaluated on their inhibitory effect towards the oxidation of hexanal for a period of 30 days. Twenty-nine aroma-active compounds with a flavour dilution (FD) factor range of 2-256 and an array of odour notes were obtained. Among them, the highest odour activities (FD ≥ 128) factors were determined for 2-acetyl furan and 2-acetylpyrrole. Other compounds with significant FD factors ≥ 32 were; 2-methylpyrazine, ethyl furfural, and 5-hydroxy methyl furfural.
Conclusion: Results of the anti-oxidative activity showed that the pyrroles exhibited the greatest antioxidant activity among all the tested heterocyclic compounds. This was followed by the furans and the pyrazines which had the least antioxidant activity.

Matched MeSH terms: Gas Chromatography-Mass Spectrometry
Potassium Alum [KAl(SO4)2∙12H2O] solid catalyst for effective and selective methoxylation production of alpha-pinene ether products

Wijayati N, Lestari LR, Wulandari LA, Mahatmanti FW, Rakainsa SK, Cahyono E, et al.

Heliyon, 2021 Jan;7(1):e06058.
PMID: 33553744 DOI: 10.1016/j.heliyon.2021.e06058

Methoxylation is a relevant technological process applied in the production of high-value α-pinene derivatives. This report investigates the use of potassium alum [KAl(SO4)2 · 12H2O] as a catalyst in the methoxylation of α-pinene. In this study, the methoxylation reaction was optimized for the highest conversion of α-pinene and selectivity, assessed for the factors, catalyst loading (0.5; 1.0; and 1.5 g), volume ratio of α-pinene: methanol (1:4, 1:7, 1:10), reaction temperature (50, 55, 60 and 65 °C), and reaction time (72, 144, 216, 288, 360 min). The highest selectivity of KAl(SO4)2∙12H2O in the methoxylation of α-pinene was achieved under an optimal condition of 1 g of catalyst loading, volume ratio of 1:10, as well as the reaction temperature and incubation time of 65 °C and 6 h, respectively. GC-MS results revealed the yields of the methoxylated products from the 98.2% conversion of α-pinene, to be 59.6%, 8.9%, and 7.1% for α-terpinyl methyl ether (TME), fenchyl methyl ether (FME), bornyl methyl ether (BME), respectively. It was apparent that a lower KAl(SO4)2∙12H2O loading (0.5-1.5 g) was more economical for the methoxylation reaction. The findings seen here indicated the suitability of the KAl(SO4)2 · 12H2O to catalyze the methoxylation of α-pinene to produce an commercially important ethers.

Matched MeSH terms: Gas Chromatography-Mass Spectrometry
Potent allelopathy and non-PSTs, non-spirolides toxicity of the dinoflagellate Alexandrium leei to phytoplankton, finfish and zooplankton observed from laboratory bioassays

Shang L, Xu Y, Leaw CP, Lim PT, Wang J, Chen J, et al.

Sci Total Environ, 2021 Aug 01;780:146484.
PMID: 33774286 DOI: 10.1016/j.scitotenv.2021.146484

The dinoflagellate genus Alexandrium has been well known for causing paralytic shellfish poisoning (PSP) worldwide. Several non-PSP toxin-producing species, however, have shown to exhibit fish-killing toxicity. Here, we report the allelopathic activity of Alexandrium leei from Malaysia to other algal species, and its toxicity to finfish and zooplankton, via laboratory bioassays. Thirteen microalgal species that co-cultured with Al. leei revealed large variability in the allelopathic effects of Al. leei on the test algae, with the growth inhibition rates ranging from 0 to 100%. The negative allelopathic effects of Al. leei on microalgae included loss of flagella and thus the motility, damages of chain structure, deformation in cell morphology, and eventually cell lysis. The finfish experienced 100% mortality within 24 h exposed to the live culture (2000-6710 cells·mL-1), while the rotifer and brine shrimp exhibited 96-100% and 90-100% mortalities within 48 h when exposed to 500-6000 cells·mL-1 of Al. leei. The mortality of the test animals depended on the Al. leei cell density exposed, leading to a linear relationship between mortality and cell density for the finfish, and a logarithmic relationship for the two zooplankters. When exposed to the treatments using Al. leei whole live culture, cell-free culture medium, extract of algal cells in the f/2-Si medium, extract of methanol, and the re-suspended freeze-and-thaw algal cells, the test organisms (Ak. sanguinea and rotifers) all died at the cell density of 8100 cells·mL-1 within 24 h. Toxin analyses by HILIC-ESI-TOF/MS and LC-ESI-MS/MS demonstrated that Al. leei did not produce PSP-toxins and 13-desmethyl spirolide C. Overall, our findings demonstrated potent allelopathy and toxicity of Al. leei, which do not only pose threats to the aquaculture industry, fisheries, and marine ecosystems but may also play a part role in the population dynamics and bloom formation of this species.

Matched MeSH terms: Tandem Mass Spectrometry
Fulltext Metabolome analysis and chemical profiling of Indonesian royal jellies as the raw material for cosmetic and bio-supplement products

Sari E, Mahira KF, Patel DN, Chua LS, Pratami DK, Sahlan M

Heliyon, 2021 May;7(5):e06912.
PMID: 34013079 DOI: 10.1016/j.heliyon.2021.e06912

Royal jellies (RJs) possess moisturizing, emulsifying, and stabilizing properties, and several pharmacological activities have also been found to be present, which make them an ideal component for cosmetic and skin care products. However, despite the abundant efficacies, there is a lack of studies that explore the chemical composition of RJ using metabolome analysis. Furthermore, an evaluation of the chemical composition of Indonesian RJs collected from different regions has yet to be carried out. Therefore, the main objective of this study was to identify any differences in the chemical composition of such RJs. Chemical profiling was also carried out to enable more targeted utilization based on the actual compositions. Chemical profiling is also important given the rich Indonesian biodiversity and the high dependence of the RJ compositions on the botanical source. In this research, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used as part of an untargeted metabolomics approach. From the chemical profiling, >30 compounds were identified across four RJ samples. The major constituents of the samples were found to be oligosaccharides, fatty acids, and adenosine monophosphate derivatives. Meanwhile, sucrose and planteose were found to be highest in the samples from Banjarnegara and Kediri, whereas dimethyloctanoic acid was found to be unique to the sample from Banjarnegara. It was also discovered that the RJs from Demak and Tuban contained more organic fatty acids and oligosaccharides than the other samples. Although the sample from Demak demonstrated good potential for use in the cosmetic, skin care, and bio-supplement industries, the higher abundance of fatty acids and oligosaccharides in the sample from Tuban indicated that it is perhaps the most suitable RJ for use in this field.

Matched MeSH terms: Mass Spectrometry
Comparative database search engine analysis on massive tandem mass spectra of pork-based food products for halal proteomics

Amir SH, Yuswan MH, Aizat WM, Mansor MK, Desa MNM, Yusof YA, et al.

J Proteomics, 2021 06 15;241:104240.
PMID: 33894373 DOI: 10.1016/j.jprot.2021.104240

Mass spectrometry-based proteomics relies on dedicated software for peptide and protein identification. These software include open-source or commercial-based search engines; wherein, they employ different algorithms to establish their scoring and identified proteins. Although previous comparative studies have differentiated the proteomics results from different software, there are still yet studies specifically been conducted to compare and evaluate the search engine in the field of halal analysis. This is important because the halal analysis is often using commercial meat samples that have been subjected to various processing, further complicating its analysis. Thus, this study aimed to assess three open-source search engines (Comet, X! Tandem, and ProteinProspector) and a commercial-based search engine (ProteinPilot™) against 135 raw tandem mass spectrometry data files from 15 types of pork-based food products for halal analysis. Each database search engine contained high false-discovery rate (FDR); however, a post-searching algorithm called PeptideProphet managed to reduce the FDR, except for ProteinProspector and ProteinPilot™. From this study, the combined database search engine (executed by iProphet) reveals a thorough protein list for pork-based food products; wherein the most abundant proteins are myofibrillar proteins. Thus, this proteomics study will aid the identification of potential peptide and protein biomarkers for future precision halal analysis. SIGNIFICANCE: A critical challenge of halal proteomics is the availability of a database to confirm the inferential peptides as well as proteins. Currently, the established database such as UniProtKB is related to animal proteome; however, the halal proteomics is related to the highly processed meat-based food products. This study highlights the use of different database search engines (Comet, X! Tandem, ProteinProspector, and ProteinPilot™) and their respective algorithms to analyse 135 raw tandem mass spectrometry data files from 15 types of pork-based food products. This is the first attempt that has compared different database search engines in the context of halal proteomics to ensure the effectiveness of controlling the FDR. Previous studies were just focused on the advantages of a certain algorithm over another. Moreover, other previous studies also have mainly reported the use of mass spectrometry-based shotgun proteomics for meat authentication (the most similar field to halal analysis), but none of the studies were reported on halal aspects that used samples originated from highly processed food products. Hence, a systematic comparative study is duly needed for a more comprehensive and thorough proteomics analysis for such samples. In this study, our combinatorial approach for halal proteomics results from the different search engines used (Comet, X! Tandem, and ProteinProspector) has successfully generated a comprehensive spectral library for the pork-based meat products. This combined spectral library is freely available at https://data.mendeley.com/datasets/6dmm8659rm/3. Thus far, this is the first and new attempt at establishing a spectral library for halal proteomics. We also believe this study is a pioneer for halal proteomics that aimed at non-conventional and non-model organism proteomics, protein analytics, protein bioinformatics, and potential biomarker discovery.

Matched MeSH terms: Tandem Mass Spectrometry
Fulltext Entamoeba histolytica: Proteomics Bioinformatics Reveal Predictive Functions and Protein-Protein Interactions of Differentially Abundant Membrane and Cytosolic Proteins

Azmi N, Othman N

Membranes (Basel), 2021 May 21;11(6).
PMID: 34063994 DOI: 10.3390/membranes11060376

Amoebiasis is caused by Entamoeba histolytica and ranked second for parasitic diseases causing death after malaria. E. histolytica membrane and cytosolic proteins play important roles in the pathogenesis. Our previous study had shown several cytosolic proteins were found in the membrane fraction. Therefore, this study aimed to quantify the differential abundance of membrane and cytosolic proteins in membrane versus cytosolic fractions and analyze their predicted functions and interaction. Previous LC-ESI-MS/MS data were analyzed by PERSEUS software for the differentially abundant proteins, then they were classified into their functional annotations and the protein networks were summarized using PantherDB and STRiNG, respectively. The results showed 24 (44.4%) out of the 54 proteins that increased in abundance were membrane proteins and 30 were cytosolic proteins. Meanwhile, 45 cytosolic proteins were found to decrease in abundance. Functional analysis showed differential abundance proteins involved in the molecular function, biological process, and cellular component with 18.88%, 33.04% and, 48.07%, respectively. The STRiNG server predicted that the decreased abundance proteins had more protein-protein network interactions compared to increased abundance proteins. Overall, this study has confirmed the presence of the differentially abundant membrane and cytosolic proteins and provided the predictive functions and interactions between them.

Matched MeSH terms: Tandem Mass Spectrometry
Fulltext A Review of Gelatin Source Authentication Methods

Hameed AM, Asiyanbi-H T, Idris M, Fadzillah N, Mirghani MES

Trop Life Sci Res, 2018 Jul;29(2):213-227.
PMID: 30112151 MyJurnal DOI: 10.21315/tlsr2018.29.2.15

Gelatin is a very popular pharmaceutical and food ingredient and the most studied ingredient in Halal researches. Interest in source gelatin authentication is based on religious and cultural beliefs, food fraud prevention and health issues. Seven gelatin authentication methods that have been developed include: nucleic acid based, immunochemical, electrophoretic analysis, spectroscopic, mass-spectrometric, chromatographic-chemometric and chemisorption methods. These methods are time consuming, and require capital intensive equipment with huge running cost. Reliability of gelatin authentication methods is challenged mostly by transformation of gelatin during processing and close similarities among gelatin structures. This review concisely presents findings and challenges in this research area and suggests needs for more researches on development of rapid authentication method and process-transformed gelatins.

Matched MeSH terms: Mass Spectrometry
Fulltext Characterization of agarwood incense using gas chromatography - mass spectrometry (GC-MS) coupled with Solid Phase Micro Extraction (SPME) and Gas Chromatography - Flame Ionization Detector (GC-FID)

Nurlaila Ismail, Mohd Hezri Fazalul Rahiman, Mohd Nasir Taib, Mastura Ibrahim, Seema Zareen, Saiful Nizam Tajuddin

Scientific Research Journal, 2015;12(2):0-0.
MyJurnal

This paper presents the application of Solid Phase Micro Extraction (SPME) coupled with Gas Chromatography - Mass Spectrometry (GC-MS) and Gas Chromatography - Flame Ionization Detector (GC-FID) in characterizing the agarwood incense. The work involved three types of SPME fibres at 30 minute sampling time. The fibres are 50/30 μm divinylbenzene-carboxen-polydimethysiloxane (DVB-CAR-PDMS), 65 μm polydi methylsiloxane-divinylbenzene (PDMS-DVB) and 85 μm carboxen-polydimethyl siloxane (CAR-PDMS). The results showed that among the many compounds extracted by GC-MS coupled with SPME, six compounds were substantially found in high quality agarwood incense due to their high percentage area (%). They are β-maaliene, α-elemol, β-selinene, 10-epi-γ-eudesmol, agarospirol and caryophellene oxide. The finding offers a new approach for establishing the volatile profile of agarwood incense components as well as for agarwood grading and discrimination.

Matched MeSH terms: Gas Chromatography-Mass Spectrometry
Fulltext Liquefaction of Mukah Balingian Coal via Semi-continuous Two-stage Solvent Flow Reactor System

M.A.M. Ishak, M.T. Safian, Z.A. Ghani, K. Ismail

ASM Science Journal, 2013;7(1):7-17.
MyJurnal

Solvent flow reactor system was introduced into the extraction system to increase the system efficiency and enhance the extraction yield by adding fresh solvent during the extraction processes. The liquefaction experiment was carried out at various flow-rates (1, 3 and 5 ml/min), reaction times (30, 45 and 60 min) and reaction temperatures (300ºC, 350ºC, 400ºC, 420ºC and 450ºC) with tetralin as solvent. Despite the ability of adding fresh solvent into the extraction process, the conversion of oil+gas was still considered to be low as there was ~25% of coal extracts left to be converted into low molecular weight compounds. One possible option to increase the oil yield is by applying catalyst that will further break up the coal extracts into small molecular weight compounds. In this study, a second reactor was introduced consisting of catalyst (NiSiO2) assuming that the catalyst would interact more effectively with coal extracts rather than the coal itself. In the
absence of catalyst, the oil yield was 55%. By introducing the Ni catalyst, the oil yield increased by 15%. Further analysis of GCMS showed that the oil from catalytic liquefaction gave out more low molecular weight compounds in comparison to the un-catalytic liquefaction oil.

Matched MeSH terms: Gas Chromatography-Mass Spectrometry
Fulltext Ichthyotoxic properties and essential oils of Syzygium Malaccense (Myrtaceae)

Intan S. Ismail, NorAkmar Ismail, Nordin Lajis

Pertanika Journal of Science & Technology, 2010;18(1):-.
MyJurnal

The preliminary ichthyotoxic test on all parts of Syzygium malaccense (Myrtaceae) revealed that the leaves fraction was the most ichthyotoxic against tilapia-fish (Tilapia oreochromis). Three compounds, namely ursolic acid (1), β-sitosterol (2) and sitost-4-en-3-one (3), were isolated and their structures were elucidated with the aid of spectroscopic data and comparison with previously reported investigations. However none of these compounds gave any significant ichthyotoxicity. The volatile constituents of the leaves and fruit were determined by Gas Chromatography-Mass Spectrometer (GC-MS), with 180 and 203 compounds being identified in the aroma concentrates, respectively.

Matched MeSH terms: Gas Chromatography-Mass Spectrometry
Fulltext Relating descriptive sensory analysis to gas chromatography/mass spectrometry of palm sugars using partial least squares regression

Wan Aida, W.M., Ho, C.W., Maskat, M.Y., Osman, H.

International Food Research Journal, 2008;15(1):35-45.
MyJurnal

Sensory attributes of four different palm sugars were related to gas chromatography/mass spectrometry (GC/MS) analysis using partial least squares regression (PLS). The sweet caramel and burnt-like sensory attributes were strongly associated with 2-furfural and 2-furan methanol volatile compounds. The sensory scores for roasty and nutty were also associated with the GC/MS ratings for roasty and nutty-like aroma by its highest scores obtained from 2-ethyl-5-methyl pyrazine, 2,5-dimethyl pyrazine and 2,3-dimethyl pyrazine volatile compounds along the PC1 dimension. PLS analysis did not show correlation for the character impact compound furaneol, 2-ethyl-3,5-dimethyl pyrazine (EDMP) and 2,3-diethyl-5-methyl pyrazine (DEMP), which are perceived to be responsible for the sweet caramel-like and roasty/nutty attributes of palm sugars, respectively. This lack of relationship could partially be explained by covariance among the sensory ratings for the samples.

Matched MeSH terms: Gas Chromatography-Mass Spectrometry
Sequential Hybrid Three-Dimensional Gas Chromatography with Accurate Mass Spectrometry: A Novel Tool for High-Resolution Characterization of Multicomponent Samples

Yan D, Wong YF, Whittock SP, Koutoulis A, Shellie RA, Marriott PJ

Anal Chem, 2018 04 17;90(8):5264-5271.
PMID: 29575899 DOI: 10.1021/acs.analchem.8b00142

A novel sequential three-dimensional gas chromatography-high-resolution time-of-flight mass spectrometry (3D GC-accTOFMS) approach for profiling secondary metabolites in complex plant extracts is described. This integrated system incorporates a nonpolar first-dimension (1Dnp) separation step, prior to a microfluidic heart-cut (H/C) of a targeted region(s) to a cryogenic trapping device, directly followed by the rapid reinjection of a trapped solute into a polar second-dimension (2DPEG) column for multidimensional separation (GCnp-GCPEG). For additional separation, the effluent from 2DPEG can then be modulated according to a comprehensive 2D GC process (GC×GC), using an ionic liquid phase as a third-dimension (3DIL) column, to produce a sequential GCnp-GCPEG×GCIL separation. Thus, the unresolved or poorly resolved components, or regions that require further separation, can be precisely selected and rapidly transferred for additional separation on 2D or 3D columns, based on the greater separation realized by these steps. The described integrated system can be used in a number of modes, but one useful approach is to target specific classes of compounds for improved resolution. This is demonstrated through the separation and detection of the oxygenated sesquiterpenes in hop ( Humulus lupulus L.) essential oil and agarwood ( Aquilaria malaccensis) oleoresin. Improved resolution and peak capacity were illustrated through the progressive comparison of the tentatively identified components for GCnp-GCPEG and GCnp-GCPEG×GCIL methods. Relative standard deviations of intraday retentions (1 tR, 2 tR,, and 3 tR) and peak areas of ≤0.01, 0.07, 0.71, and 7.5% were achieved. This analytical approach comprising three GC column selectivities, hyphenated with high-resolution TOFMS detection, should be a valuable adjunct for the improved characterization of complex plant samples, particularly in the area of plant metabolomics.

Matched MeSH terms: Gas Chromatography-Mass Spectrometry
Fulltext Peptidomics dataset: Blood plasma and serum samples of healthy donors fractionated on a set of chromatography sorbents

Arapidi G, Osetrova M, Ivanova O, Butenko I, Saveleva T, Pavlovich P, et al.

Data Brief, 2018 Jun;18:1204-1211.
PMID: 29900295 DOI: 10.1016/j.dib.2018.04.018

Blood as connective tissue potentially contains evidence of all processes occurring within the organism, at least in trace amounts (Petricoin et al., 2006) [1]. Because of their small size, peptides penetrate cell membranes and epithelial barriers more freely than proteins. Among the peptides found in blood, there are both fragments of proteins secreted by various tissues and performing their function in plasma and receptor ligands: hormones, cytokines and mediators of cellular response (Anderson et al., 2002) [2]. In addition, in minor amounts, there are peptide disease markers (for example, oncomarkers) and even foreign peptides related to pathogenic organisms and infection agents. To propose an approach for detailed peptidome characterization, we carried out an LC-MS/MS analysis of blood serum and plasma samples taken from 20 healthy donors on a TripleTOF 5600+ mass-spectrometer. We prepared samples based on our previously developed method of peptide desorption from the surface of abundant blood plasma proteins followed by standard chromatographic steps (Ziganshin et al., 2011) [3]. The mass-spectrometry peptidomics data presented in this article have been deposited to the ProteomeXchange Consortium (Deutsch et al., 2017) [4] via the PRIDE partner repository with the dataset identifier PXD008141 and 10.6019/PXD008141.

Matched MeSH terms: Tandem Mass Spectrometry
Fulltext Metabolite profiles of callus and cell suspension cultures of mangosteen

Jamil SZMR, Rohani ER, Baharum SN, Noor NM

3 Biotech, 2018 Aug;8(8):322.
PMID: 30034986 DOI: 10.1007/s13205-018-1336-6

Callus was induced from mangosteen (Garcinia mangostana L.) young purple-red leaves on Murashige and Skoog basal medium with various combinations of plant growth regulators. Murashige and Skoog medium with 4.44 µM 6-benzylaminopurine and 4.52 µM 2,4-dichlorophenoxyacetic acid was the best for friable callus induction. This friable callus was used for the initiation of cell suspension culture. The effects of different combinations of 6-benzylaminopurine and 2,4-dichlorophenoxyacetic acid, carbon sources and inoculum sizes were tested. It was found that combination of 2.22 µM 6-benzylaminopurine + 2.26 µM 2,4-dichlorophenoxyacetic acid, glucose (30 g/l) and 1.5 g/50 ml inoculum size was the best for cell growth. Callus and cell suspension cultures were then treated either with 100 µM methyl jasmonate as an elicitor for 5 days, or 0.5 g/l casein hydrolysate as an organic supplement for 7 days. Metabolites were then extracted and profiled using liquid chromatography-time of flight mass spectrometry. Multivariate discriminant analyses revealed significant metabolite differences (P ≤ 0.05) for callus and suspension cells treated either with methyl jasmonate or casein hydrolysate. Based on MS/MS data, methyl jasmonate stimulated the production of an alkaloid (thalsimine) and fatty acid (phosphatidyl ethanolamine) in suspension cells while in callus, an alkaloid (thiacremonone) and glucosinolate (7-methylthioheptanaldoxime) was produced. Meanwhile casein hydrolysate stimulated the production of alkaloids such as 3ß,6ß-dihydroxynortropane and cis-hinokiresinol and triterpenoids such as schidigerasaponin and talinumoside in suspension cells. This study provides evidence on the potential of secondary metabolite production from in vitro culture of mangosteen.

Matched MeSH terms: Tandem Mass Spectrometry
Fulltext Heterocyclic aromatic amines in deep fried lamb meat: The influence of spices marination and sensory quality

S J, Iqbal SZ, Talib NH, Hasnol ND

J Food Sci Technol, 2016 Mar;53(3):1411-7.
PMID: 27570265 DOI: 10.1007/s13197-015-2137-0

The present study was focused to investigate the effect of selected spices (turmeric, torch ginger, lemongrass and curry leaves) on the formation of heterocyclic amines (HCAs, IQx, MeIQ, MeIQx, DiMeIQx, IQ, harman, norharman, and AαC) in deep fried lamb meat. Meat samples were marinated with optimized levels of turmeric (4 %), 10 % each of torch ginger, lemon grass, curry leaves at medium (70 °C) and well done (80 °C) doneness temperatures. The concentration of HCAs in deep fried meat samples were analysed using LC-MS/MS technique. The results revealed that torch ginger (10 %) has reduced 74.8 % of Me1Qx (1.39 to 0.35 ng/g) at medium doneness, followed by the 64.7 % reduction, using curry leaves and turmeric at medium degree of doneness. Torch ginger has reduced 86.6 % of AαC (2.59 to 0.40 ng/g) at well done doneness. The most prevalence level of HCAs was found in deep fried meat i.e. DiMeIQ (3.69 ng/g) at well done doneness. The sensory evaluation, using a 7 point hedonic test design for colour and texture in deep fried meat samples were resulted in a preferred color of golden brown and slightly tough texture. The use of local spices in marinating of deep fried lamb meat samples will certainly inhibit/reduce the level of these toxic and harmful HCAs.

Matched MeSH terms: Tandem Mass Spectrometry
Pyrolysis of corn stalk biomass briquettes in a scaled-up microwave technology

Salema AA, Afzal MT, Bennamoun L

Bioresour Technol, 2017 Jun;233:353-362.
PMID: 28285228 DOI: 10.1016/j.biortech.2017.02.113

Pyrolysis of corn stalk biomass briquettes was carried out in a developed microwave (MW) reactor supplied with 2.45GHz frequency using 3kW power generator. MW power and biomass loading were the key parameters investigated in this study. Highest bio-oil, biochar, and gas yield of 19.6%, 41.1%, and 54.0% was achieved at different process condition. In terms of quality, biochar exhibited good heating value (32MJ/kg) than bio-oil (2.47MJ/kg). Bio-oil was also characterised chemically using FTIR and GC-MS method. This work may open new dimension towards development of large-scale MW pyrolysis technology.

Matched MeSH terms: Gas Chromatography-Mass Spectrometry
Pseudo-nitzschia nanaoensis sp. nov. (Bacillariophyceae) from the Chinese coast of the South China Sea

Li Y, Dong HC, Teng ST, Bates SS, Lim PT

J Phycol, 2018 12;54(6):918-922.
PMID: 30270437 DOI: 10.1111/jpy.12791

Pseudo-nitzschia nanaoensis sp. nov. is described from waters around Nan'ao Island (South China Sea), using morphological data and molecular evidence. This species is morphologically most similar to P. brasiliana, but differs by a denser arrangement of fibulae, interstriae, and poroids, as well as by the structure of the valvocopula and the narrow second band. Pseudo-nitzschia nanaoensis constitutes a monophyletic lineage and is well differentiated from other species on the LSU and ITS2 sequence-structure trees. Pseudo-nitzschia nanaoensis makes up the basal node on the LSU tree, and forms a sister clade with a group of P. pungens and P. multiseries on the ITS2 tree. The ability of cultured strains to produce domoic acid was assessed, including its possible induction by the presence of a copepod and brine shrimp, by liquid chromatography-tandem mass spectrometry. However, no strains showed detectable domoic acid.

Matched MeSH terms: Tandem Mass Spectrometry

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