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The control of corruption and energy efficiency relationship: an empirical note

Ozturk I, Al-Mulali U, Solarin SA

Environ Sci Pollut Res Int, 2019 Jun;26(17):17277-17283.
PMID: 31012074 DOI: 10.1007/s11356-019-05016-1

This study aims at exploring the impact of corruption control on energy efficiency in 60 countries categorized by income: lower middle (LMI), upper middle (UMI), and high (HI). Panel methodology was utilized taking the period of 2000-2017. As cross-sectional dependence is confirmed among the tested equations, the Pesaran (J Appl Econ 22(2):265-312, 2007) unit root test and the augmented mean group estimator proposed by Eberhardt and Teal (2010) were utilized to overcome this matter. The results in general indicate that the lower the corruption is, the more the energy efficiency for all income group economies. Moreover, renewable energy reduces energy efficiency in lower-middle income and high-income economies while its effect is positive in middle-income economies. In addition, the environmental Kuznets curve (EKC) found to be present in all income group economies. Lastly, causality relationships among energy efficiency, corruption, and GDP were present mostly in upper-middle income and high-income economies. From the results, it was recommended that the countries from all income groups should increase their corruption control for the purpose of enhancing energy efficiency.

Matched MeSH terms: Guanosine Diphosphate
Capital- and consumption-led growth: case study on Malaysia, Singapore, Japan, and Zimbabwe

Mohd Azlan Abdul Majid, Mariam Setapa, Noorita Mohammad

CREAM : Current Research in Malaysia (Penyelidikan Terkini di Malaysia), 2012;1(1):151-187.
MyJurnal

Tujuan kertas kerja ini adalah untuk mengetahui hubungan, kesan ekonomi semasa dan faktor pencemaran terhadap modal, perbelanjaan terhadap pertumbuhan ekonomi terutamanya kepada Malaysia dan dibandingkan dengan negara seperti Jepun, Singapura dan Zimbabwe. Persoalan utama adalah sama ada modal dan perbelanjaan dapat berterusan menyumbangkan kepada pertumbuhan ekonomi ataupun tidak. Untuk mengkaji perhubungan, kami gunakan Test Unit Root (TUR); Augmented Dickey-Fuller Method (ADF), Phillip-Perron Test (PP) dan Kwiatkowski-Phillip-Schmidt-Shin (KPSS), Test Johansen Cointegration (TJC), diikuti Vector Error Correction Model (VECM) dan akhir sekali Test Granger Causality (TGC). Tujuan pengujian ini adalah untuk mengukur perhubungan, kestabilan dan kesahihan model. Kedua, untuk mengkaji kesan impak ekonomi kami gunakan Impulse Respond dan ujian Variance Decomposition yang menyelidik kesan berapa banyak dan berapa panjang model bertindakbalas kepada kesan Kegawatan Ekonomi Asia pada 1997 dan Kesan Serangan Pengganas di Amerika Syarikat pada September 2011. Ketiga kami juga megukur kesan perbelanjaan isi rumah dan kerajaan terhadap alam persekitaran. Kertas kerja ini mendapati, ia menyokong idea modal penjana pertumbuhan tetapi natijah perbelanjaan penjana pertumbuhan adalah berbeda mengikut tahap pertumbuhan. Di mana Malaysia dan Singapura menyokong sepenuhnya tetapi Jepun dan Zimbabwe hanya menyokong separa sahaja. Apabila kami mengukur impak kesan ekonomi pula, di dapati pada negara Zimbabwe tiada kesan antara krisis pada tahun 1997 ataupun pada 2001. Tetapi impak pada negara seperti Jepun, Malaysia dan Singapura pula berhadapan dalam posisi tidak baik pada krisis tahun 1997 tetapi keadaan yang baik pula pada krisis tahun 2001. Akhir sekali kami juga bangunkan theori pencemaran dimana untuk negara Zimbabwe ke semua pembolehubah seperti Perbelanjaan isi rumah (HC), Perbelanjaan Kerajaan (GC) dan Modal Tetap Kasar (GFC) tidak mempengaruhi pencemaran iaitu Karbon Dioksida (CO2). Tetapi natijah untuk negara Malaysia dan Singapura pula hanya HC mencipta CO2, tidak untuk GC dan GFC. Dan pengakhiran sekali Negara Jepun pula semua pembolehubah seperti GC,HC dan GFC mencipta pencemaran. Kesimpulannya krisis yang berlaku pada negara Amerika Syarikat pada tahun 2001 memberi kesan terus pada Negara Amerika tetapi tidak pada negara seperti Malaysia, Jepun dan Singapura, walhal negara-negara ini menadapat lambakan di dalam pasaran modal akibat perubahan ketidaktentuan dalam ekonomi Amerika seterusnya meningkatkan secara langsung Keluaran Dalam Negara Kasar (GDP). Walaubagaimanapun negara Zimbabwe tidak mempunyai kesan langsung sama ada sebelum atupun selepas masalah krisis ekonomi ini. Apabila kertas kerja ini mengkaji daripada sudut pembangunan lestari didapati Negara Zimbabwe seperti HC dan GC tidaklah mencemar seperti negara Malaysia dan Singapura yang menunjukkan HC punca pencemaran dan bukannya GC yang mungkin disebabkan skala ekonominya yang lebih kecil. Untuk negara Jepun pula disebabkan negara ini mempunyai skala ekonomi yang lebih besar HC dan GC adalah agen kepada pencemaran. Implikasinya model ini sepatutnya diambil kira dalam pembuat keputusan terutamanya untuk meninkatkan pertumbuhan ekonomi.

Matched MeSH terms: Guanosine Diphosphate
Fulltext Epigallocatechin gallate (EGCG) alleviates vascular dysfunction in angiotensin II-infused hypertensive mice by modulating oxidative stress and eNOS

Mohd Sabri NA, Lee SK, Murugan DD, Ling WC

Sci Rep, 2022 Oct 21;12(1):17633.
PMID: 36271015 DOI: 10.1038/s41598-022-21107-5

Epigallocatechin gallate (EGCG) has been shown to have antihypertensive activity. However, the role of epigallocatechin gallate (EGCG) in improving vascular function via modulation of endothelial nitric oxide synthase (eNOS) in hypertensive subjects is not well researched. Angiotensin II-infused hypertensive mice (8-10 weeks old) received EGCG (50 mg/kg/day) for 14 days via oral gavage. The arterial systolic blood pressure (SBP) was measured using the tail-cuff method every three days. At the end of the treatment, the vascular reactivity of the isolated aortae was studied using wire myographs. The level of nitric oxide (NO), cyclic guanosine monophosphate (cGMP) and tetrahydrobiopterine (BH4) were determined using assay kits while the presence of proteins (NOS, p-eNOS and NOx-2) were determined using by Western blotting. In vivo treatment with EGCG for 14 days significantly attenuated the increase in SBP, alleviated the vascular dysfunction, increased the vascular cGMP and BH4 level as well as the expression of p-eNOS and decreased elevated ROS level and NOx-2 protein in angiotensin II-infused hypertensive mice. Collectively, treatment with EGCG in hypertensive mice exerts a blood pressure lowering effect which is partly attributed to the improvement in the vascular function due to its ability to reduce vascular oxidative stress in the aortic tissue leading to a decrease in eNOS uncoupling thus increasing NO bioavailability.

Matched MeSH terms: Guanosine Monophosphate/metabolism; Guanosine Monophosphate/pharmacology
Plant Rabs and the role in fruit ripening

Lawson T, Mayes S, Lycett GW, Chin CF

Biotechnol Genet Eng Rev, 2018 Oct;34(2):181-197.
PMID: 29902948 DOI: 10.1080/02648725.2018.1482092

Fruit ripening is a complex developmental process that involves the synthesis and modification of the cell wall leading up to the formation of an edible fruit. During the period of fruit ripening, new cell wall polymers and enzymes are synthesized and trafficked to the apoplast. Vesicle trafficking has been shown to play a key role in facilitating the synthesis and modification of cell walls in fruits. Through reverse genetics and gene expression studies, the importance of Rab guanosine triphosphatases (GTPases) as integral regulators of vesicle trafficking to the cell wall has been revealed. It has been a decade since a rich literature on the involvement of Rab GTPase in ripening was published. Therefore, this review sets out to summarize the progress in studies on the pivotal roles of Rab GTPases in fruit development and sheds light on new approaches that could be adopted in the fields of postharvest biology and fruit-ripening research.

Matched MeSH terms: Guanosine
Fulltext Carbon cess on fuel consumption

Loganathan, P.

ASM Science Journal, 2011;5(1):75-76.
MyJurnal

In December 2009, at the UN Climate Change Conference COP 15 in Copenhagen, the Prime Minister of Malaysia, the Rt. Hon. Mohd Najib Abdul Razak, had announced that “Malaysia is adopting an indicator of a voluntary reduction of up to 40% in terms of emissions intensity of GDP by the year 2020 compared to 2005 levels. This indicator is conditional on receiving the transfer of technology and finance of adequate and effective levels from Annex 1 partners, that correspond to what is required in order to achieve this indicator”. Malaysia also needs to decouple its GDP from its current relatively high carbon-based energy demand. In trying to meet both these targets, we need to intensify our research efforts in energy-based areas. As government funding in R&D is limited as the aim is for research to be private-sector driven, and with the grim global economic scenario today, it is most unlikely that Malaysia will get any assistance financially or technologically from Annex 1 nations. It is therefore proposed that Malaysia considers implementing a dedicated carbon cess on petroleum products to fund R&D in the country. (Copied from article).

Matched MeSH terms: Guanosine Diphosphate
Fulltext Effect of Vietnamese coriander (Persicaria odorata), turmeric (Curcuma longa) and asam gelugor (Garcinia atroviridis) leaf on the microbiological quality of gulai tempoyak paste

Abdul Aris, M. H., Lee, H. Y., Hussain, N., Ghazali, H., Nordin, W. N., Mahyudin, N. A.

International Food Research Journal, 2015;22(4):1657-1661.
MyJurnal

The objective of this study was to determine microbiological quality of gulai tempoyak paste (GTP) added with three different leaf; Vietnamese coriander, turmeric and asam gelugor. The GTP was cooked for 10 minutes with control temperature (60-70°C) and the leaf were added at 2, 5 and 8 minutes during the cooking time to give exposure times of 8, 5 and 2 minutes of the leaf to GTP. GTP without addition of leaf was treated as control and all the prepared GTPs were stored at 30°C for 2 days before analysed using total plate count (TPC) and yeast and mould count (YMC). The addition of asam gelugor leaf to GTP for 5 minutes of the cooking period significantly (p > 0.05) reduced TPC (log10 3.54 CFU/g) compared to Vietnamese coriander (log10 4.67 CFU/g) and turmeric leaf (log10 4.70 CFU/g). Asam gelugor leaf also showed a significant difference in TPC reduction (log10 4.44 CFU/g) when added to GTP for 8 minutes compared to Vietnamese coriander (log10 5.10 CFU/g), but was insignificant to turmeric leaf (log10 4.71 CFU/g). In conclusion, there are significant effects on microbiological quality of GTP when added with Vietnamese coriander, turmeric and asam gelugor leaf at different exposure time based on TPC and YMC.

Matched MeSH terms: Guanosine Triphosphate
Different pontic design for porcelain fused to metal fixed dental prosthesis: Contemporary guidelines and practice by general dental practitioners

Raza Kazmi SM, Iqbal Z, Muneer MU, Riaz S, Zafar MS

Eur J Dent, 2018 8 28;12(3):375-379.
PMID: 30147402 DOI: 10.4103/ejd.ejd_232_18

Objective: The current study aimed to assess the knowledge and practice of pontic design selection by the general dental practitioners (GDPs) in the light of contemporary guidelines.
Materials and Methods: This cross-sectional study was conducted among the GDPs of Karachi. A questionnaire was designed to collect data from 100 GDPs. The questionnaire included general/demographic information (practitioner's education, experience, and place of practice) and an average number of fixed prosthesis constructed by the GDP. The questionnaire was further categorized to evaluate the knowledge/practice of pontic design selection and latest recommendations.
Results: For the maxillary anterior segment, the ridge lap pontic was the most common (32%) followed by the modified ridge lap (28%). In the maxillary posterior segment, the ridge lap pontic was the most common (37%) followed by sanitary design (34%). For the mandibular anterior segment, the modified ridge lap (50%) was the most common followed by ridge lap pontic (17%). In case of the mandibular posterior segment, the sanitary design (34%) was the most common followed by ridge lap pontic (30%).
Conclusions: The pontic design selection for the fixed prosthesis is a neglected domain. The contemporary guidelines are not followed with full spirit by the GDPs leading to wide variations in the pontic design selection.

Matched MeSH terms: Guanosine Diphosphate
Fulltext Crowdfunding as financial option for small and medium enterprises (smes) in nigeria

Kazaure, Mansur Ahmed, Abdul Rashid abdullah

The Pertanika Journal of Scholarly Research Reviews, 2018;4(3):89-96.
MyJurnal

Small and medium scale enterprises (SMEs) act as a promoter of economic development of the developed and under developed nations. Developing countries like Nigeria that, require sustainable economic growth and development must pay attention to the SMEs sectors in order to reduce unemployment, poverty and improve the standard leaving of its society. Unfortunately, in Nigeria, SMEs have underperformed despite that, 90% of the Nigerian businesses are small scales but their contribution to the Nigerian GDP is below 10%. The main reason for this low contribution of SMEs to Nigerian GDP could be attribute to poor funding, lack of management expertise and lack of access to modern technology among others, with proper finance the SMEs can acquire modern technology and employed qualified staff. Thus, this paper focuses on crowdfunding as financial option for Nigerian SMEs. However, the paper is a conceptual framework.

Matched MeSH terms: Guanosine Diphosphate
Pharmacological modulation of oncogenic Ras by natural products and their derivatives: Renewed hope in the discovery of novel anti-Ras drugs

Quah SY, Tan MS, Teh YH, Stanslas J

Pharmacol Ther, 2016 06;162:35-57.
PMID: 27016467 DOI: 10.1016/j.pharmthera.2016.03.010

Oncogenic rat sarcoma (Ras) is linked to the most fatal cancers such as those of the pancreas, colon, and lung. Decades of research to discover an efficacious drug that can block oncogenic Ras signaling have yielded disappointing results; thus, Ras was considered "undruggable" until recently. Inhibitors that directly target Ras by binding to previously undiscovered pockets have been recently identified. Some of these molecules are either isolated from natural products or derived from natural compounds. In this review, we described the potential of these compounds and other inhibitors of Ras signaling in drugging Ras. We highlighted the modes of action of these compounds in suppressing signaling pathways activated by oncogenic Ras, such as mitogen-activated protein kinase (MAPK) signaling and the phosphoinositide-3-kinase (PI3K) pathways. The anti-Ras strategy of these compounds can be categorized into four main types: inhibition of Ras-effector interaction, interference of Ras membrane association, prevention of Ras-guanosine triphosphate (GTP) formation, and downregulation of Ras proteins. Another promising strategy that must be validated experimentally is enhancement of the intrinsic Ras-guanosine triphosphatase (GTPase) activity by small chemical entities. Among the inhibitors of Ras signaling that were reported thus far, salirasib and TLN-4601 have been tested for their clinical efficacy. Although both compounds passed phase I trials, they failed in their respective phase II trials. Therefore, new compounds of natural origin with relevant clinical activity against Ras-driven malignancies are urgently needed. Apart from salirasib and TLN-4601, some other compounds with a proven inhibitory effect on Ras signaling include derivatives of salirasib, sulindac, polyamine, andrographolide, lipstatin, levoglucosenone, rasfonin, and quercetin.

Matched MeSH terms: Guanosine Triphosphate/metabolism
Studies on the supramolecular complex of a guanosine with beta-cyclodextrin and evaluation of its anti-proliferative activity

Prabu S, Samad NA, Ahmad NA, Jumbri K, Raoov M, Rahim NY, et al.

Carbohydr Res, 2020 Nov;497:108138.
PMID: 32911205 DOI: 10.1016/j.carres.2020.108138

The behavior of the inclusion behavior of guanosine (GU) with beta-cyclodextrin (β-CD) in the liquid, solid and virtual state were investigated. The absorption and fluorescence spectral were used to determine the inclusion behavior in liquid state. FT-IR, NMR, TGA, DSC, PXRD and FESEM techniques were used to investigate the inclusion behavior in solid-state, meanwhile the virtual state studies are done by molecular docking. The solid inclusion complex (GU: β-CD) was prepared by using the co-precipitation method. The binding constant (K) of (GU: β-CD) was calculated by using Benesi-Hildebrand. Besides that, the 1:1 stoichiometric ratio of inclusion complex was confirmed by using the Benesi-Hildebrand plot and Job's plot of continuous variation method. The most preferable model of GU: β-CD that suggested via molecular docking studies was in good agreement with experimental results. The inclusion complex of GU: β-CD exerted its toxicity effects towards HepG2 cell lines based on the reduced number of cell viability and lowest IC50 value compared to the GU and β-CD viability.

Matched MeSH terms: Guanosine
Fulltext Computational approaches: discovery of GTPase HRas as prospective drug target for 1,3-diazine scaffolds

Kumar S, Sharma D, Narasimhan B, Ramasamy K, Shah SAA, Lim SM, et al.

BMC Chem, 2019 Dec;13(1):96.
PMID: 31355369 DOI: 10.1186/s13065-019-0613-8

Heterocyclic 1,3-diazine nucleus is a valuable pharmacophore in the field of medicinal chemistry and exhibit a wide spectrum of biological activities. PharmMapper, a robust online tool used for establishing the target proteins based on reverse pharmacophore mapping. PharmMapper study is carried out to explore the pharmacological activity of 1,3-diazine derivatives using reverse docking program. PharmMapper, an open web server was used to recognize for all the feasible target proteins for the developed compounds through reverse pharmacophore mapping. The results were analyzed via molecular docking with maestro v11.5 (Schrodinger 2018-1) using GTPase HRas as possible target. The molecular docking studies displayed the binding behavior of 1,3-diazine within GTP binding pocket. From the docking study compounds s3 and s14 showed better docked score with anticancer potency against cancer cell line (HCT116). Hence, the GTPase HRas may be the possible target of 1,3-diazine derivatives for their anticancer activity where the retrieved information may be quite useful for developing rational drug designing. Furthermore the selected 1,3-diazine compounds were evaluated for their in vitro anticancer activity against murine macrophages cell line. 1,3-Diazine compounds exhibited good selectivity of the compounds towards the human colorectal carcinoma cell line instead of the murine macrophages. The toxicity study of the most active compounds was also performed on non cancerous HEK-293 cell line.

Matched MeSH terms: Guanosine Triphosphate
Fulltext Cost of type 2 diabetes mellitus in selected developing countries

Wan Norlida Ibrahim, Syed Aljunid, Aniza Ismail

Malaysian Journal of Public Health Medicine, 2010;10(2):68-71.
MyJurnal

Increasing prevalence of chronic diseases is a major contributor for rapid rise in healthcare cost in developing countries since the last decade. It was estimated that around 54% of deaths in developing countries are due to chronic non-communicable diseases which is predicted to rise by 65% by 2030. Diabetes mellitus is among the most prevalent chronic diseases suffered by more than 180 million people worldwide. By 2030 it is estimated that around 400 million people in the world will be afflicted with diabetes. Annual deaths attributable to diabetes are probably as high as 3 million with more than 80% occur in developing countries. India, China and Indonesia are three countries in the Asian region with most number of people with diabetes. The total number of cases in these three countries is expected to increase more than double from 61 million in 2000 to 163 million in 2030. China and India will suffer cumulative GDP loss of 13.8% and 16.7% respectively, over the next ten year period. Assessing economic burden of diabetes is a challenging task for researchers because identification of direct and indirect cost of the disease is often complex since patients with diabetes also suffers from other complications and co-morbidities. In conclusion, the heavy economic burden of diabetes pose major challenges to health policy makers in developing countries to assess the current approach in managing this chronic disease. Serious efforts should be made on focusing and up-scaling activities on health promotion and prevention of diabetes so that to provide a more cost-effective solution to this condition with huge and increasing economic loss.

Matched MeSH terms: Guanosine Diphosphate
Employing a CGE model in analysing the environmental and economy-wide impacts of CO2emission abatement policies in Malaysia

Yahoo M, Othman J

Sci Total Environ, 2017 Apr 15;584-585:234-243.
PMID: 28152460 DOI: 10.1016/j.scitotenv.2017.01.164

The impact of global warming has received much international attention in recent decades. To meet climate-change mitigation targets, environmental policy instruments have been designed to transform the way goods and services are produced as well as alter consumption patterns. The government of Malaysia is strongly committed to reducing CO2gas emissions as a proportion of GDP by 40% from 2005 levels by the year 2020. This study evaluates the economy-wide impacts of implementing two different types of CO2emission abatement policies in Malaysia using market-based (imposing a carbon tax) and command-and-control mechanism (sectoral emission standards). The policy simulations conducted involve the removal of the subsidy on petroleum products by the government. A carbon emission tax in conjunction with the revenue neutrality assumption is seen to be more effective than a command-and-control policy as it provides a double dividend. This is apparent as changes in consumption patterns lead to welfare enhancements while contributing to reductions in CO2emissions. The simulation results show that the production of renewable energies is stepped up when the imposition of carbon tax and removal of the subsidy is augmented by revenue recycling. This study provides an economy-wide assessment that compares two important tools for assisting environment policy makers evaluate carbon emission abatement initiatives in Malaysia.

Matched MeSH terms: Guanosine Diphosphate
Fulltext Reverse pharmacophore mapping and molecular docking studies for discovery of GTPase HRas as promising drug target for bis-pyrimidine derivatives

Kumar S, Singh J, Narasimhan B, Shah SAA, Lim SM, Ramasamy K, et al.

Chem Cent J, 2018 Oct 22;12(1):106.
PMID: 30345469 DOI: 10.1186/s13065-018-0475-5

BACKGROUND: Pyrimidine is an important pharmacophore in the field of medicinal chemistry and exhibit a broad spectrum of biological potentials. A study was carried out to identify the target protein of potent bis-pyrimidine derivatives using reverse docking program. PharmMapper, a robust online tool was used for identifying the target proteins based on reverse pharmacophore mapping. The murine macrophage (RAW 264.7) and human embryonic kidney (HEK-293) cancer cell line used for selectivity and safety study.
METHODS: An open web server PharmMapper was used to identify the possible target of the developed compounds through reverse pharmacophore mapping. The results were analyzed and validated through docking with Schrodinger v9.6 using 10 protein GTPase HRas selected as possible target. The docking studies with Schrödinger validated the binding behavior of bis-pyrimidine compounds within GTP binding pocket. MTT and sulforhodamine assay were used as antiproliferative activity.
RESULTS AND DISCUSSION: The protein was found one of the top scored targets of the compound 18, hence, the GTPase HRas protein was found crucial to be targeted for competing cancer. Toxicity study demonstrated the significant selectivity of most active compounds, 12, 16 and 18 showed negligible cell toxicity at their IC50 concentration.
CONCLUSION: From the results, we may conclude that GTPase HRas as a possible target of studied bis-pyrimidine derivatives where the retrieved information may be quite useful for rational drug designing.

Matched MeSH terms: Guanosine Triphosphate
Fulltext River pollution relationship to the national health indicated by under-five child mortality rate: a case study in Malaysia

Mohd Aqmin Abdul Wahab, Adzleen Mohmood, Nur Khaliesah Jamadon, Amir Syahir

Bioremediation Science and Technology Research, 2015;3(1):20-26.
MyJurnal

The trend of global under-five child mortality rate showed a steady decrease at -3.14% per year
approaching the United Nations millennium development goals target (-4.0% reduction per year
from 1990-2015). This rate is usually inversely proportional to the nations GDP. However, the
progress in the recent years (2006-2012) in Malaysia has raised a disturbing concern with an
annual average increment of +0.81% per year. Thus, there is an urgent need to identify the
reasons of such risky ecosystem. By analyzing recorded data on river pollution from 2007-
2012, we found that river pollution has high correlation with the gross domestic products, not at
the same year, but for two years prior that indicates a lag time. We also found that under-five
child mortality has strong correlation with the river pollutions. The development-pollutionhealth
triangular cycle needs to be put in a balanced to ensure the nation prosperity and
sustainability of the nation.

Matched MeSH terms: Guanosine Diphosphate
Efficacy of purified nucleotide supplements on the growth performance and immunity of hybrid striped bass Morone chrysops x Morone saxatilis

de Cruz CR, Yamamoto FY, Ju M, Chen K, Velasquez A, Gatlin DM

Fish Shellfish Immunol, 2020 Mar;98:868-874.
PMID: 31751660 DOI: 10.1016/j.fsi.2019.11.046

Fishmeal is being increasingly replaced in aquatic animal diets with alternative plant protein feedstuffs such as soybean meal which have lower concentrations of nucleotides; therefore, supplemental sources of exogenous nucleotides in diets could become increasingly important. A 9-week feeding trial was conducted with triplicate groups of juvenile hybrid striped bass (average initial body weight ± standard deviation, 5.6 ± 0.1 g) to determine the effects of supplementing single purified nucleotides on the growth performance and immune parameters. The basal diet, which utilized menhaden fishmeal (25%) and soybean meal (75%) as protein sources, contained 44% protein, 10% lipid and an estimated digestible energy level of 3.5 kcal g-1. Single additions of 5'- adenosine monophosphate (AMP), 5'- uridine monophosphate (UMP), 5'- cytidine monophosphate (CMP), 5'- guanosine monophosphate (GMP), and 5'- inosine monophosphate (IMP) disodium salts (Chem-Impex International, Wood Dale, Illinois, USA) were evaluated with each nucleotide added to the basal diet at 0.5% of dry weight at the expense of cellulose. A positive control diet in this trial was a diet containing 5'- AMP from Sigma-Aldrich also supplemented at 0.5% by weight. Results showed significantly (P 0.05) was detected in whole-body proximate composition and protein retention of fish fed any of the dietary treatments. The respiratory burst of whole blood phagocytes also was significantly (P

Matched MeSH terms: Guanosine Monophosphate
Fulltext Antinociceptive Activity of Methanol Extract of Muntingia calabura Leaves and the Mechanisms of Action Involved

Sani MH, Zakaria ZA, Balan T, Teh LK, Salleh MZ

Evid Based Complement Alternat Med, 2012;2012:890361.
PMID: 22611437 DOI: 10.1155/2012/890361

Muntingia calabura L. (family Elaeocarpaceae) has been traditionally used to relieve various pain-related ailments. The present study aimed to determine the antinociceptive activity of methanol extract of M. calabura leaves (MEMC) and to elucidate the possible mechanism of antinociception involved. The in vivo chemicals (acetic acid-induced abdominal constriction and formalin-, capsaicin-, glutamate-, serotonin-induced paw licking test) and thermal (hot plate test) models of nociception were used to evaluate the extract antinociceptive activity. The extract (100, 250, and 500 mg/kg) was administered orally 60 min prior to subjection to the respective test. The results obtained demonstrated that MEMC produced significant (P < 0.05) antinociceptive response in all the chemical- and thermal-induced nociception models, which was reversed after pretreatment with 5 mg/kg naloxone, a non-selective opioid antagonist. Furthermore, pretreatment with L-arginine (a nitric oxide (NO) donor), N(G)-nitro-L-arginine methyl esters (L-NAME; an inhibitor of NO synthase (NOS)), methylene blue (MB; an inhibitor of cyclic-guanosine monophosphate (cGMP) pathway), or their combination also caused significant (P < 0.05) change in the intensity of the MEMC antinociception. In conclusion, the MEMC antinociceptive activity involves activation of the peripheral and central mechanisms, and modulation via, partly, the opioid receptors and NO/cGMP pathway.

Matched MeSH terms: Guanosine Monophosphate
The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes

Chew BL, Fisk ID, Fray R, Tucker GA, Bodi Z, Ferguson A, et al.

Plant Cell Rep, 2017 Jan;36(1):81-87.
PMID: 27662835 DOI: 10.1007/s00299-016-2058-z

KEY MESSAGE: This study highlights the changes in umami-related nucleotide and glutamate levels when the AMP deaminase gene was elevated in transgenic tomato. Taste is perceived as one of a combination of five sensations, sweet, sour, bitter, salty, and umami. The umami taste is best known as a savoury sensation and plays a central role in food flavour, palatability, and eating satisfaction. Umami flavour can be imparted by the presence of glutamate and is greatly enhanced by the addition of ribonucleotides, such as inosine monophosphate (IMP) and guanosine monophosphate (GMP). The production of IMP is regulated by the enzyme adenosine monophosphate (AMP) deaminase which functions to convert AMP into IMP. We have generated transgenic tomato (Solanum lycopersicum) lines over expressing AMP deaminase under the control of a fruit-specific promoter. The transgenic lines showed substantially enhanced levels of AMP deaminase expression in comparison to the wild-type control. Elevated AMP deaminase levels resulted in the reduced accumulation of glutamate and increased levels of the umami nucleotide GMP. AMP concentrations were unchanged. The effects on the levels of glutamate and GMP were unexpected and are discussed in relation to the metabolite flux within this pathway.

Matched MeSH terms: Guanosine Monophosphate/metabolism
Low-temperature and long-time heating regimes on non-volatile compound and taste traits of beef assessed by the electronic tongue system

Ismail I, Hwang YH, Joo ST

Food Chem, 2020 Aug 01;320:126656.
PMID: 32224424 DOI: 10.1016/j.foodchem.2020.126656

The influence of temperature-time combinations on non-volatile compound and taste traits of beef semitendinosus muscles tested by the electronic tongue was studied. Single-stage sous-vide at 60 and 70 °C (6 and 12 h), and two-stage sous-vide that sequentially cooked at 45 °C (3 h) and 60 °C (either 3 or 9 h) were compared with traditional cooking at 70 °C (30 min). Umami was better explained in the given model of partial least squares regression than astringency, sourness, saltiness, bitterness, and richness. Sous-vide at 70 °C for 12 h characterized the most umami, likely adenosine-5'-monophosphate (AMP) and guanosine-5'-monophosphate (GMP) as significant contributors. Two-stage sous-vide projected higher histidine, leucine, inosine, and hypoxanthine with the astringent and sour taste significant after 6 and 12 h cooking, respectively. Equivalent umami concentration (EUC) between umami amino acids and umami nucleotides showed a strong relationship to umami taste assessed by the electronic tongue.

Matched MeSH terms: Guanosine Monophosphate/analysis
Regulation of G protein signaling by the 70kDa heat shock protein

Lim WK, Kanelakis KC, Neubig RR

Cell Signal, 2013 Feb;25(2):389-96.
PMID: 23153586 DOI: 10.1016/j.cellsig.2012.11.002

G protein-coupled receptors (GPCRs) transduce extracellular signals to the interior of the cell by activating membrane-bound guanine nucleotide-binding regulatory proteins (G proteins). An increasing number of proteins have been reported to bind to and regulate GPCRs. We report a novel regulation of the alpha(2A) adrenergic receptor (α(2A)-R) by the ubiquitous stress-inducible 70kDa heat shock protein, hsp70. Hsp70, but not hsp90, attenuated G protein-dependent high affinity agonist binding to the α(2A)-R in Sf9 membranes. Antagonist binding was unchanged, suggesting that hsp70 uncouples G proteins from the receptor. As hsp70 did not bind G proteins but complexed with the α(2A)-R in intact cells, a direct interaction with the receptor seems likely. In the presence of hsp70, α(2A)-R-catalyzed [(35)S]GTPγS binding was reduced by approximately 70%. In contrast, approximately 50-fold higher concentrations of hsp70 were required to reduce agonist binding to the stress-inducible 5-hydroxytryptamine(1A) receptor (5-HT(1A)-R). In heat-stressed CHO cells, the α(2A)-R was significantly uncoupled from G proteins, coincident with an increased localization of hsp70 at the membrane. The contrasting effect of hsp70 on the α(2A)-R compared to the 5-HT(1A)-R suggests that during stress, upregulation of hsp70 may attenuate signaling from specific GPCRs as part of the stress response to foster survival.

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